Effects of different carbohydrate levels on glycolipid metabolism and liver health of channel catfish (Ictalurus punctatus)

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-06-16 DOI:10.1016/j.aqrep.2025.102921

Xiaochen Ma , Qisheng Lu , Guoli Han , Jinglu Jia , Jingyue Cao , Haokun Liu , Junyan Jin , Zhimin Zhang , Yunxia Yang , Xiaoming Zhu , Dong Han , Shouqi Xie

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Abstract

This study evaluated the effects of dietary carbohydrate levels on glycolipid metabolism and liver health of channel catfish. Six isonitrogenous and isolipidic diets with corn starch contents of 10 % (C10), 15 % (C15), 20 % (C20), 25 % (C25), 30 % (C30) and 35 % (C35) were set up. After 8-week feeding trial, there was no significant difference in the growth performance of all groups (P > 0.05). The study showed that C30 and C35 groups significantly increased plasma glucose levels, activated the expression of glycolysis-related genes (gk, hk, pklr, pfk) and mitochondrial oxidative phosphorylation related genes (ndufa1, sdhb, sdha, cytb-c1, cox, atp5fa1, atp5f1b), and repressed the expression of gluconeogenesis-related genes (pc, fbp, pepck, g6p) in the channel catfish (P < 0.05). The upregulation of glycogen synthesis-related genes (ppp1r3g, ppp1r3c, ppp1r3b, gys, gnl, ugp2a, ugp2b) contributed to a significant increase in hepatic glycogen content. In addition, C30 and C35 groups significantly increased the expression of pentose phosphate pathway related genes (g6pd, 6gpd), while C10 to C25 groups glucose metabolism-related pathways did not exhibit significant differences. Hepatic triglyceride and cholesterol results showed that C30 and C35 groups induced excessive lipogenesis, activated the expression of lipogenesis-related genes (acc, acly, vldlr) and cholesterol synthesis-related genes (srebf2, hmgcr, sqle). In C30 and C35 groups also increased the hepatic ALT, AST and MDA levels, activated the expression of injury-related genes (a-sma, tgf-β), inflammation-related genes (tnf-α, il-1β, il-6) and apoptosis-related genes (baxb, caspase-3, caspase −10) (P < 0.05). C35 group significantly further increased the expression levels of stress genes such as hamp, hsp70 and hsp90 (P < 0.05), while there were no significant differences in the levels of lactate and cortisol in all groups (P > 0.05). In conclusion, our results suggest that dietary carbohydrate levels less than 30 % will not impair the health and growth performance of the channel catfish.

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不同碳水化合物水平对斑点鲶鱼糖脂代谢及肝脏健康的影响

本研究评价了饲料碳水化合物水平对通道鲶鱼糖脂代谢和肝脏健康的影响。设置玉米淀粉含量分别为10 % （C10）、15 % （C15）、20 % （C20）、25 % （C25）、30 % （C30）和35 % （C35）的6种等氮等脂饲粮。饲喂8周后，各组生长性能无显著差异（P >； 0.05）。研究表明，C30和C35组显著提高了通道鲶鱼的血浆葡萄糖水平，激活了糖酵解相关基因（gk、hk、plklr、pfk）和线粒体氧化磷酸化相关基因（ndufa1、sdhb、sdha、cytbc1、cox、atp5fa1、atp5f1b）的表达，抑制了糖异生相关基因（pc、fbp、pepck、g6p）的表达（P <； 0.05）。糖原合成相关基因（ppp1r3g、ppp1r3c、ppp1r3b、gys、gnl、ugp2a、ugp2b）的上调导致肝糖原含量显著升高。此外，C30和C35组戊糖磷酸途径相关基因（g6pd、6gpd）的表达显著增加，而C10和C25组葡萄糖代谢相关途径的表达无显著差异。肝脏甘油三酯和胆固醇结果显示，C30和C35组诱导脂肪过度生成，激活脂肪生成相关基因（acc、acly、vldlr）和胆固醇合成相关基因（srebf2、hmgcr、sqle）的表达。C30和C35组肝脏ALT、AST和MDA水平升高，损伤相关基因（a-sma、tgf-β）、炎症相关基因（tnf-α、il-1β、il-6）和凋亡相关基因（baxb、caspase-3、caspase- 10）表达激活（P <； 0.05）。C35组进一步显著提高了应激基因hamp、hsp70、hsp90的表达水平（P <； 0.05），各组间乳酸、皮质醇水平无显著差异（P <； 0.05）。综上所述，饲粮碳水化合物水平低于30 %不会影响通道鲶鱼的健康和生长性能。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.