Effects of commercial feeds and natural diets on the culture performance and gut microbiota of Scylla paramamosain

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-06-14 DOI:10.1016/j.aqrep.2025.102922

Xia Zheng , Dongping Zhou , Sixiang Wang , Xiaojing Dong , Chaoyue Wang , Zehao Li , Lei Liu , Changkao Mu , Chunlin Wang

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引用次数: 0

Abstract

In this study, we systematically investigated how different feed combinations affect the growth performance and gut microbiota composition of the mud crab Scylla paramamosain in both indoor cement tanks and outdoor earthen ponds. We employed high-throughput 16S rRNA sequencing to profile the bacterial communities, placing particular emphasis on correlations between feed nutritional components and key microbial taxa. Firmicutes, Proteobacteria, Bacteroidetes, and Cyanobacteria emerged as the predominant phyla. Crabs fed locally sourced natural feeds from Dongying showed relatively minor shifts in community structure, whereas those receiving artificial formulated feed exhibited significant divergences, as evidenced by principal coordinate analysis (PCoA) and non-metric multidimensional scaling (NMDS). In indoor cement tank trials, no significant differences were found in Chao1 among groups. However, the combination of Mactra chinensis + Synechogobius hasta + Trachypenaeus curvirostris group significantly elevated Pielou_e and Shannon diversity indices (P < 0.05), while the Simpson index was higher in both this group and the S. hasta + T. curvirostris + corn + soybean meal combination (P < 0.05). Similar trends were observed in outdoor earthen pond trials, where the M. chinensis + S. hasta + T. curvirostris group and the S. hasta + T. curvirostris + corn + soybean meal group exhibited significantly higher diversity indices (P < 0.05). Moreover, several indicator genera (e.g., OM190, Terrisporobacter, Paraclostridium) were positively correlated with specific nutritional components, implying that balanced feed formulas can promote beneficial bacteria, potentially supporting molting and growth. Collectively, the M. chinensis + S. hasta + T. curvirostris feed combination yielded the most favorable results, highlighting its potential for enhancing both crab growth and gut microbiota diversity. These findings advance our understanding of how feed composition shapes the gut microbiota in S. paramamosain and offer a theoretical basis for optimizing feed formulations, thereby contributing to sustainable aquaculture in Dongying and similar regions.

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商品饲料和天然饲料对Scylla paramamosain培养性能和肠道菌群的影响

本研究系统研究了不同饵料组合对室内水泥池和室外泥池中顺滑石蟹生长性能和肠道菌群组成的影响。我们采用高通量16S rRNA测序来分析细菌群落，特别强调饲料营养成分与关键微生物类群之间的相关性。厚壁菌门、变形菌门、拟杆菌门和蓝藻门是主要的门。主坐标分析（PCoA）和非度量多维标度分析（NMDS）结果表明，东营本地天然饲料饵料饵料的蟹类群落结构变化相对较小，而人工配方饲料饵料的蟹类群落结构变化明显。室内水泥罐试验中，Chao1组间无显著差异。而Mactra chinensis + Synechogobius hasta + Trachypenaeus curvirostris组显著提高了Pielou_e和Shannon多样性指数（P <； 0.05），Simpson指数高于S. hasta + T组。curvirostris + 玉米+ 豆粕组合（P <； 0.05）在室外土池试验中也观察到类似的趋势。直到+ T。curvirostris组和S. hasta + T。curvirostris + 玉米+ 豆粕组多样性指数显著高于对照组（P <； 0.05）。此外，一些指标属（如OM190、恐怖杆菌、副梭状芽孢杆菌）与特定营养成分正相关，这意味着平衡的饲料配方可以促进有益菌，可能支持换毛和生长。总的来说，M. chinensis + S。直到+ T。曲弧菌饲料组合取得了最有利的结果，突出了其促进螃蟹生长和肠道微生物群多样性的潜力。这些发现促进了我们对饲料成分如何影响顺滑索菌肠道菌群的理解，并为优化饲料配方提供了理论依据，从而为东营和类似地区的可持续水产养殖做出贡献。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.