Aquaculture Reports最新文献

{"title":"Low-salinity stress induces JNK-mediated apoptosis and inflammation in the gills of the pearl oyster, Pinctada fucata martensii","authors":"Lining Ma ,&nbsp;Haiying Liang ,&nbsp;Qiyuan Zhang ,&nbsp;Guiling Yin ,&nbsp;Runlin Lei","doi":"10.1016/j.aqrep.2026.103446","DOIUrl":"10.1016/j.aqrep.2026.103446","url":null,"abstract":"<div><div>In the stress response, c-Jun N-terminal kinase (JNK) mediated inflammatory response plays a key role in maintaining homeostasis and removing damaged cells. In this study, <em>Pinctada fucata martensii</em> was used as the object, and the stress response mechanism of gill tissue under low-salinity stress ( 20ppt) was systematically discussed by using the techniques of fluorescence quantification, transcriptomics, histopathology and apoptosis analysis. The results showed that low-salinity stress significantly induced the up-regulation of Pm<em>JNK</em> gene expression, especially in gill and hepatopancreas. Transcriptome analysis of three groups of normal salinity ( 30 ppt), low salinity stress ( 20 ppt) and inhibitor ( 20 ppt + JNK inhibitor) showed that low salinity stress resulted in a large number of differentially expressed genes significantly enriched in important biological pathways such as immune inflammatory response, apoptosis and metabolic reprogramming. Among them, the NF-κB signaling pathway was significantly activated, and the cytochrome P450 metabolic pathway also changed significantly, suggesting that the body may adapt to the low-salt environment through metabolic reprogramming. The observation of tissue sections further showed that low-salinity stress damaged the gill structure, which was characterized by disordered arrangement of epithelial cells and destruction of basement membrane integrity. JNK inhibitor treatment can alleviate the above tissue damage, indicating that JNK pathway plays a central role in low salt-induced structural damage. TUNEL experiments confirmed that low-salinity stress significantly promoted apoptosis of gill cells, and inhibition of JNK pathway could reduce the level of apoptosis, which functionally verified the role of <em>JNK</em> in regulating low salt-induced apoptosis. This study revealed the core position of JNK signaling pathway in the response to low-salinity stress in <em>Pinctada fucata martensii</em>, and elucidated its process of mediating tissue damage by coordinating multiple mechanisms such as immune inflammation, apoptosis and metabolic reprogramming. It provides a new theoretical basis for further understanding the molecular mechanism of marine shellfish responding to environmental stress.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103446"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential of food encapsulation technologies in aquaculture: Applications, innovations, and future perspectives","authors":"Jiayi Li ,&nbsp;Jiancao Gao ,&nbsp;Haojun Zhu ,&nbsp;Liping Cao ,&nbsp;Jinliang Du ,&nbsp;Jun Gao ,&nbsp;Gangchun Xu","doi":"10.1016/j.aqrep.2026.103448","DOIUrl":"10.1016/j.aqrep.2026.103448","url":null,"abstract":"<div><div>The modern aquaculture is increasingly dependent on functional feeds, prophylactic formulations, and additives for water-quality regulation. However, the efficacy of many bioactive agents is constrained by multiple factors, including oxidative degradation with loss of activity, rapid dissolution and leaching in aquatic environments, and discrepancies between laboratory dosing protocols and on-farm feeding practices. Food-grade encapsulation technologies—such as micro-/nanoencapsulation, emulsions, liposomes, and hydrogel carriers—are now mature and can protect labile compounds while enabling controlled release. Their translation to aquaculture, nevertheless, requires targeted optimization that accounts for open-water exposure, mechanical stresses during feed processing, and pronounced interspecies differences in digestive physiology. This review synthesizes recent advances in food-grade carriers and fabrication processes relevant to aquaculture, emphasizing three priorities: (1) enhancing nutrient stability and controlled delivery; (2) improving mucosal targeting efficiency of oral vaccines and therapeutics; and (3) developing water-regulation functions using sustained-release nutrients and recyclable adsorbent materials. We further provide a critical assessment of key translational bottlenecks, including species-specific release kinetics, carrier aggregation and biofouling in complex aquatic matrices, life-cycle toxicological evaluation for non-target organisms, and constraints on cost and scalability for industrial deployment. Finally, we outline future priorities, including standardized in vitro digestion/leaching assays, cross-species benchmarking frameworks, and stimuli-responsive “smart” carriers to accelerate the transition from proof-of-concept to field-ready products.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103448"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of ceRNA regulatory networks in the spleen of iridovirus-infected leopard coral grouper (Plectropomus leopardus)","authors":"Lei Wang ,&nbsp;Ruiqi Guo ,&nbsp;Jinfeng Liu ,&nbsp;Min Tian ,&nbsp;Yunji Xiu ,&nbsp;Run Wang ,&nbsp;Zemin Bai ,&nbsp;Songlin Chen","doi":"10.1016/j.aqrep.2026.103438","DOIUrl":"10.1016/j.aqrep.2026.103438","url":null,"abstract":"<div><div>Leopard coral grouper (<em>Plectropomus leopardus</em>) is among the most important economic marine fish in China. However, iridovirus infection has caused severe loss in aquaculture, creating an urgent need to discover the immune mechanism in response to viral infection. Thus, this study involved whole transcriptome analysis in spleen of iridovirus-infected leopard coral grouper using high-throughput sequencing. Assembly and annotation identified 1407 mRNAs, 75 miRNAs, 21 circRNAs, and 264 lncRNAs exhibited significantly different expressions in the spleen of iridovirus-infected leopard coral grouper. Further, the study identified two groups of competing endogenous RNA (ceRNAs) networks, as lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA, that including five novel lncRNAs, four novel circRNAs, five hub miRNAs and 25 mRNAs. This study presents the first demonstration of the ceRNA crosstalk in spleen of iridovirus-infected leopard coral grouper, providing new molecular targets for investigating the antivirus mechanism of marine fish.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103438"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary microalgae supplementation at different levels on Macrobrachium rosenbergii insect meal-based diets: Antioxidant capacity, hepatopancreatic morphology, and carotenoid retention","authors":"Hadil Elsayed ,&nbsp;Victor Rosas ,&nbsp;Razan Khalifa ,&nbsp;Shaikha Y. Al-Sayegh ,&nbsp;Larissa M.K. Britz ,&nbsp;Juan R.B. Ramirez ,&nbsp;Mhd Ikhwanuddin ,&nbsp;Ponnumony Vethamony ,&nbsp;Jassim A. Al-Khayat","doi":"10.1016/j.aqrep.2026.103431","DOIUrl":"10.1016/j.aqrep.2026.103431","url":null,"abstract":"<div><div>The replacement of fish meal with insect meal (IM) in aquafeeds is increasingly promoted as a sustainable strategy to reduce dependence on marine resources. However, IM based diets are inherently low in carotenoids and natural antioxidants, which can limit pigmentation, oxidative stability, and final product quality in crustaceans. Although IM inclusion in shrimp diets has been widely reported, to our knowledge, no study has evaluated whether supplementation with bioactive ingredients can functionally enhance IM based diets. This study investigated whether carotenoid-rich microalgae could compensate for these limitations in black soldier fly meal-based diets for the giant freshwater prawn (<em>Macrobrachium rosenbergii</em>). Experimental diets were supplemented with 2.5 % or 5 % <em>Arthrospira platensis</em> (Spirulina) or <em>Haematococcus pluvialis</em> and evaluated during a 60-day nursery trial. Growth performance, carotenoid retention, colorimetric traits, antioxidant status, lipid peroxidation, and hepatopancreas histology were assessed.</div><div>Growth performance did not differ among treatments (P &gt; 0.05). In contrast, microalgae supplementation significantly increased astaxanthin, lutein, and β-carotene retention, confirming that IM alone does not adequately support pigmentation-related quality traits. The highest total carotenoid concentration was observed in prawns fed the H5 diet (35.4 μg g⁻¹). Spirulina primarily enhanced raw prawn coloration, whereas <em>H. pluvialis</em> significantly affected cooked prawn color. Antioxidant capacity was highest and lipid peroxidation lowest in the S2 treatment, while higher inclusion levels (5 %) were associated with elevated oxidative stress.</div><div>These findings demonstrate IM capabilities can be effectively enhanced through moderate microalgae supplementation. Inclusion at 2.5 % optimised coloration, carotenoid retention, and antioxidant status, without compromising growth, supporting the development of high-quality, sustainable insect-based prawn diets.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103431"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes of intestinal microbiota and metabolites in Huso dauricus under heat stress and their potential relationship with heat stress resistance","authors":"Ruoyu Wang ,&nbsp;Yutao Li ,&nbsp;Zheng He ,&nbsp;Bo Sun ,&nbsp;Yining Zhang ,&nbsp;Sihan Wang ,&nbsp;Bo Ma ,&nbsp;Dingchen Cao ,&nbsp;Zhipeng Sun ,&nbsp;Nianmin Wang ,&nbsp;Wei Hu ,&nbsp;Shijian Xu ,&nbsp;Ying Zhang","doi":"10.1016/j.aqrep.2026.103439","DOIUrl":"10.1016/j.aqrep.2026.103439","url":null,"abstract":"<div><div>Ongoing global warming has intensified heat stress challenges for fish, especially cold-water species. This study focused on 4-month-old kaluga sturgeon (<em>Huso dauricus</em>), conducting heat stress experiments by raising the temperature from 19℃ to 25℃ at 1℃ per hour, with 19℃ as the control. Anatomical observations assessed heat stress injury in the gill, liver, and intestine, while pathological tissue sections evaluated intestinal injuries. Microbiome and metabolomics techniques explored the intestinal microbiota and metabolites' response to heat stress and their relationship with heat stress resistance. Results indicated that half of the sturgeons can adapt to heat stress without sustaining injury. Significant changes were observed in the intestinal microbiota due to heat stress, with a decrease in beneficial bacteria, such as <em>Clostridium sensu stricto 1</em> and <em>Candidatus Arthromitus</em>, and an increase in the harmful bacterium <em>Plesiomonas</em>. Notably, this dysbiosis manifested differentially between groups: heat-resistant sturgeon were enriched in the beneficial <em>Cetobacterium</em>, whereas heat-stressed individuals harbored significantly more <em>Plesiomonas</em>. Changes in metabolite concentrations associated with energy supply, exemplified by Uridine diphosphate-N-acetylglucosamine, and with cell death pathways, as seen in gamma-Glutamylcysteine, were also noted in this study. The decline of <em>Clostridium sensu stricto 1</em> and its related metabolite phenylacetic acid likely contributed to the rise in harmful bacteria and inflammatory injuries. <em>Cetobacterium</em> and its related metabolite lithocholyltaurine may enhance heat stress resistance. These findings offer a theoretical basis for improving fish heat stress resistance through microbiome regulation and suggest potential strategies for the aquaculture industry to address climate change.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103439"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary naringenin reduces high-fat diet-induced liver injury in largemouth bass by regulating intestinal microbiota and activating the ERα/NLRP3 pathway","authors":"Shiyang Gao,&nbsp;Weijun Chen,&nbsp;Hongtao Ren,&nbsp;Yong Huang,&nbsp;Chunnuan Zhang,&nbsp;Xiaochan Gao,&nbsp;Ping Sun","doi":"10.1016/j.aqrep.2026.103425","DOIUrl":"10.1016/j.aqrep.2026.103425","url":null,"abstract":"<div><div>The NLR family pyrin domain containing 3 (NLRP3) inflammasome’s activation is essential for fish liver damage brought on by a high-fat diet. Despite naringenin's potential to block NLRP3 inflammasome activation, its preventive effects against fish liver damage caused by a high-fat diet and the underlying processes are yet unknown. To address this, six experimental diets were formulated: a normal-fat diet (10 % lipid, control) and five high-fat diets (18 % lipid, HF) supplemented with 0 %, 0.005 %, 0.01 %, 0.02 %, and 0.04 % naringenin. Each diet was randomly given to three tanks (30 fish/tank). After an eight-weeks feeding trial, the HF did not affect growth performance but induced hepatic and intestinal oxidative stress, ultrastructural damage, hepatocyte apoptosis, hepatic inflammation, and gut dysbiosis (<em>P</em> &lt; 0.05). Dietary supplementation with naringenin (0.005 %-0.02 %) significantly attenuated hepatic oxidative stress by reducing malondialdehyde (MDA) content and increasing total superoxide dismutase (T-SOD) activity, while also suppressing hepatic inflammation through downregulating the exprssion of NLRP3 inflammasome-related markers (<em>P</em> &lt; 0.05). Furthermore, 0.02 % naringenin ameliorated hepatic ultrastructure, reduced hepatocyte apoptosis, and improved gut microbiota composition by decreasing <em>Mycoplasma</em> abundance and increasing beneficial genera (e.g., <em>Bacillus</em>, <em>Lactobacillus</em>, and <em>Weissella</em>) abundance (<em>P</em> &lt; 0.05). The correlation analysis revealed that estrogen receptor alpha (ERα) was negatively correlated with NLRP3 inflammasome activation and that microbiota enriched in the 0.02 % naringenin group were positively correlated with improved liver health indicators (<em>P</em> &lt; 0.05). In conclusion, naringenin effectively alleviates HF-induced liver damage in fish by regulating oxidative stress, NLRP3 inflammasome, and gut microbiota, and may serve as a promising feed additive in aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103425"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A 25-year bibliometric analysis of tryptophan research in aquaculture: trends, themes, and opportunities for cannibalism management in Cherax quadricarinatus (Von Martens,1868)","authors":"Yuani Mundayana ,&nbsp;Zainuddin ,&nbsp;Syafiuddin Syafiuddin ,&nbsp;Laode Alhamd ,&nbsp;Tri Heru Prihadi ,&nbsp;Titin Kurniasih ,&nbsp;Dasep Hasbullah ,&nbsp;Tutik Kadarini ,&nbsp;Umi Chodrijah ,&nbsp;Amran Ronny Syam ,&nbsp;Hidayat Suryanto Suwoyo ,&nbsp;Roosganda Elizabeth","doi":"10.1016/j.aqrep.2026.103427","DOIUrl":"10.1016/j.aqrep.2026.103427","url":null,"abstract":"<div><div>Tryptophan (Trp) plays a pivotal role in aquaculture as a precursor to serotonin and melatonin, influencing neuroendocrine functions, stress response, and aggression in aquatic species. This study presents a 25-year bibliometric analysis (2000–2024) on tryptophan research in aquaculture, with a particular focus on its potential for cannibalism management in <em>Cherax quadricarinatus</em>. A total of 450 peer-reviewed articles indexed in Scopus were analyzed using Bibliometrix and VOSviewer. Results show a peak of 47 documents in 2024, and there are four main thematic clusters were identified: (1) neurophysiology and gut microbiota, (2) aquaculture performance, (3) stress and hormonal regulation, and (4) nutritional supplementation. Despite 53 studies (21.9 %) addressing aggressive behaviour and cannibalism, fewer than 5 % specifically examined freshwater crayfish. The \"neurophysiology and behaviour\" theme linked to serotonin and behavioural regulation emerges as an underexplored yet promising domain for species-specific research. These findings highlight a clear research gap and suggest promising directions for future investigation, especially in species-specific studies that integrate neuroendocrine modulation, behavioural assessments, and nutritional interventions. The current analysis offers a solid foundation for designing targeted research on functional feed strategies aimed at improving welfare and reducing aggressive behaviour in crustacean aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103427"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth prediction and feeding optimization for hybrid grouper (Epinephelus fuscoguttatus × E. lanceolatus) based on an improved bioenergetics model","authors":"Xueqian Sun ,&nbsp;Shanhong Zhang ,&nbsp;Yang Wang ,&nbsp;Zunfang Pang ,&nbsp;Xia Zhao ,&nbsp;Wensheng Li","doi":"10.1016/j.aqrep.2026.103417","DOIUrl":"10.1016/j.aqrep.2026.103417","url":null,"abstract":"<div><div>It is essential for farmers to accurately predict growth and reduce feed waste in industrial aquaculture. From a practical production perspective, an improved bioenergetic model (IBM) was proposed for hybrid grouper (<em>Epinephelus fuscoguttatus</em> × <em>E. lanceolatus</em>), which included three sub-models: growth sub-model, energy sub-model, and density sub-model. Growth and feeding data were collected from three industrial aquaculture scenarios (Study A, B, C) to validate the performance of IBM. Selected the root mean square error (<em>RMSE</em>), mean absolute percentage error (<em>MAPE</em>), and the coefficient of determination (<em>R</em>²) values to evaluate the model performance. Results indicated that there are residual bait and an imbalance in feeding by manual feeding (<em>f</em>_{<em>manual</em>}), as the model-estimated food intake by IBM (<em>f</em>_<em>IBM</em>) is less than <em>f</em>_{<em>manual</em>}; Growth prediction were relatively consistently met the target weight; Feed conversion ratios (<em>FCR</em>) obtained by IBM were 1.08, 1.08, and 1.12, respectively, all of which were lower than the manual <em>FCR</em>s of 1.2, 1.15, and 1.13; IBM achieved a higher energy retention rate during the culturing period. Meanwhile, it successfully quantified the energy metabolism and predicted the density changes during culturing period. Additionally, an integrated global sensitivity analysis was carried out on main parameters, such as temperature (<em>T</em>), satiation level (<em>s</em>), dissolved oxygen (<em>DO</em>), unionized ammonia (<em>UIA</em>), and dietary protein (<em>F</em>_<em>p</em>). Results demonstrated that <em>T</em> and <em>s</em> are two critical parameters for hybrid grouper with significant interaction between them under favorable environment. <em>DO</em> exerts the greatest influence under extreme environment. In conclusion, IBM could accurately predict fish growth and give reasonable feeding decisions. It exhibits strong practical applicability in industrial scenarios, providing a reliable tool for hybrid grouper aquaculture production.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103417"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a 50K liquid-phase SNP genotyping array to accelerate genetic breeding in Litopenaeus vannamei","authors":"Shuo Fu,&nbsp;Yuan Zhang,&nbsp;Chaoan Guo,&nbsp;Guangbo Wu,&nbsp;Jianyong Liu","doi":"10.1016/j.aqrep.2026.103419","DOIUrl":"10.1016/j.aqrep.2026.103419","url":null,"abstract":"<div><div>The high cost of genotyping remains a significant limitation to the widespread adoption of molecular breeding in aquaculture. This study aimed to develop a 50K liquid-phase Single Nucleotide Polymorphism (SNP) array for the economically important shrimp <em>Litopenaeus vannamei</em>, and to comprehensively evaluate its potential for application in genetic breeding programs. Based on whole-genome resequencing (WGS) data from 547 individuals from five commercial populations, we selected 50,031 uniformly distributed SNPs as background loci. These were combined with 951 previously identified functional SNPs related to key economic traits to construct the final 50K SNP array. We validated its technical performance by genotyping 417 offspring and assessed its practical applications through principal component analysis (PCA), genome-wide association study (GWAS) and genomic selection (GS) for body weight. The SNP array exhibited excellent performance, with a 98.21% average call rate, 98.82% average genotype concordance, and high polymorphism (97.44% of SNPs with MAF <span><math><mo>&gt;</mo></math></span> 0.05). Application validation through PCA effectively discerned population structure. GWAS using two models identified seven significant SNPs associated with body weight, implicating candidate genes with putative roles in growth and development. Most importantly, GS using the array data improved prediction accuracy for body weight by 11.24% over traditional pedigree-based methods. We also found that a marker density as low as 5 K was sufficient for achieving near-maximum prediction accuracy. This SNP array provides a powerful and cost-effective tool to accelerate genetic improvement in modern shrimp breeding programs.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103419"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influences of fishmeal substitution with protein blends on growth performance, protein digestibility, intestinal health, and gut microbiota of gibel carp (Carassius gibelio var. CAS Ⅴ) under thermal stress","authors":"Yongning Yu ,&nbsp;Zhuocheng Lou ,&nbsp;Dong Han ,&nbsp;Xiaoming Zhu ,&nbsp;Haokun Liu ,&nbsp;Zhimin Zhang ,&nbsp;Yunxia Yang ,&nbsp;Shouqi Xie ,&nbsp;Junyan Jin","doi":"10.1016/j.aqrep.2026.103426","DOIUrl":"10.1016/j.aqrep.2026.103426","url":null,"abstract":"<div><div>Temperature is a crucial environmental factor for poikilothermic aquatic species and can affect their protein utilization. This study examined the impacts of temperature (optimal 27 °C vs. high 33 °C) and protein blends (formulated at 1:1:8:2, 1:1:6:4, and 1:1:4:6 ratios using <em>Chlorella</em> meal, <em>Tenebrio molitor</em> meal, <em>Clostridium autoethanogenum</em> protein, and cottonseed protein concentrate as fishmeal substitutes) on growth performance, health status, and gut microbiota of gibel carp. Healthy fish (3.45 ± 0.03 g) were randomly distributed among 24 tanks (30 fish/tank), with each temperature condition assigned four experimental diets and each treatment replicated in three tanks for an 8-week feeding trial. High temperature (33 °C) enhanced feed intake and final body weight, but reduced feed efficiency (FE) and the apparent digestibility coefficients of crude protein and total essential amino acid. It also decreased intestinal superoxide dismutase, glutathione, and glutathione peroxidase activities, increased malondialdehyde content and elevated heat shock protein 70 (HSP70) and HSP90 levels. Moreover, it down-regulated <em>il10</em> and <em>tgfβ</em> transcription and reduced villus height with lymphocyte infiltration. Furthermore, there was an enrichment in phyla <em>Proteobacteria</em> and <em>Actinobacteriota</em> and genera <em>Mycobacterium</em>, while beneficial <em>Cetobacterium</em> abundance was decreased. Compared to fishmeal, the protein blend in a 1:1:6:4 ratio improved FE and protein retention efficiency at 33 °C. Concurrently, this blend augmented the abundance of phyla <em>Firmicutes</em> and genera <em>Clostridium_sensu_stricto_12</em>. In summary, although elevated temperature enhanced growth via hyperphagia, it substantially compromised intestinal health and nutrient utilization. This adverse effect was mitigated through nutritional intervention with the optimized 1:1:6:4 protein blend formulation.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103426"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}