Aquaculture Reports最新文献

{"title":"More than just a pinch of salt? The impact of increasing salinity on maraena whitefish in different husbandry systems","authors":"Alexander Rebl ,&nbsp;Frederike Schmachtl ,&nbsp;Ronald M. Brunner ,&nbsp;Carsten Kühn ,&nbsp;Fabian Swirplies ,&nbsp;Tom Goldammer","doi":"10.1016/j.aqrep.2025.102758","DOIUrl":"10.1016/j.aqrep.2025.102758","url":null,"abstract":"<div><div>The present study investigated the influence of different salinity concentrations on gene expression patterns in maraena whitefish (<em>Coregonus maraena</em>) from three aquaculture facilities. Over a period of 10 weeks, we compared maraena whitefish from the same batch of fertilised eggs that were kept in a freshwater-pond system and a net-cage facility in the southwestern Baltic Sea, where periodic saline intrusions from the North Sea occur. Having demonstrated differential expression of the two variants of the well-established <em>atp1a1</em> biomarker gene in gills of maraena whitefish from both habitats, we profiled the expression levels of a further 22 seawater-tolerance biomarkers in the gills, skin, head kidney and liver. The expression profiles were largely consistent across whitefish from both habitats, with only sporadic modulations. A principal component analysis revealed that these differential expression patterns were dominated by <em>eef2</em>, <em>ghr</em> and <em>wasf1.</em> A subsequent salinity experiment, carried out in a recirculating aquaculture system under constant environmental conditions, revealed that increases in salinity from 0 to 8 Practical Salinity Units (PSU) resulted in significantly elevated transcript levels of <em>fkbp5</em> and <em>atp1a1a</em> in the gills. The reduced levels of <em>eef2</em>, <em>ghr, wasf1, nampt</em> and <em>uba1</em> in the head kidney, skin or both tissues indicated a response mechanism to higher salinity (20 PSU). Altogether, only the quantification of the <em>atp1a1a</em> gene in the gill tissue provided reliable evidence of successful acclimation from low- to high-salinity water in all three facilities investigated. Based on the overall transcriptional data and the generally good fish physical constitution, we assume that maraena whitefish were not negatively affected by salinities up to 20 PSU suggesting their ability to tolerate dynamic brackish-water environments.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102758"},"PeriodicalIF":3.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing the effect of different carbon sources on growth, blood parameters, immunity, and water quality in Nile Tilapia (Oreochromis niloticus) cultured in a biofloc system","authors":"Mai H. Hussien ,&nbsp;Mohamed M. Zayd ,&nbsp;Ahmed E. Elshafey ,&nbsp;Malik M. Khalafalla ,&nbsp;Mustafa Shukry ,&nbsp;Mohamed Abdelmegeid ,&nbsp;Lamiaa A. Okasha ,&nbsp;Rabiha Seboussi ,&nbsp;Mohamed H. Aboraya ,&nbsp;Ahmed A. Elolimy","doi":"10.1016/j.aqrep.2025.102755","DOIUrl":"10.1016/j.aqrep.2025.102755","url":null,"abstract":"<div><div>Aquaculture faces significant challenges in maintaining water quality and fish health. This study evaluated the effects of different carbon sources on growth, immunity, and water quality in Nile tilapia (initial weight: 2.8 ± 0.002 g) cultured in a biofloc system over 90 days. Five treatment groups were tested, including a control group without carbon supplementation (T1) and groups supplemented with molasses (T2), vinasse (T3), starch (T4), or rice bran (T5). Results showed that molasses significantly improved (p &lt; 0.05) growth performance, feed efficiency, and hematological parameters, suggesting enhanced oxygen-carrying capacity and immune function. Additionally, molasses increased glucose, triglycerides, and albumin levels, improving liver function and energy metabolism. Immune responses, including lysozyme activity, IgM levels, and phagocytic activity, were significantly enhanced in the molasses group. Antioxidant enzyme activity was higher, and oxidative stress indicators were lower in this group. Histological analysis revealed improved intestinal and gill morphology in fish reared on molasses. Water quality parameters, such as pH, dissolved oxygen, and total ammonia nitrogen, were positively affected by vinasse and starch. Gene expression analysis indicated molasses-influenced genes associated with stress response, antioxidant activity, inflammation, and growth. These findings suggest that molasses is a promising carbon source for biofloc systems, improving growth, health, and water quality in Nile tilapia aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102755"},"PeriodicalIF":3.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of pharmacological inhibition of signal transduction pathways in steroid-induced oocyte maturation in rainbow trout","authors":"Gregory M. Weber,&nbsp;Lisa M. Radler,&nbsp;Jill E. Birkett","doi":"10.1016/j.aqrep.2025.102760","DOIUrl":"10.1016/j.aqrep.2025.102760","url":null,"abstract":"<div><div>Gonadotropins, steroids and insulin family growth factors act throughout the follicle-oocyte complex to regulate ovarian follicle maturation. Induction of oocyte maturation by the maturation inducing hormone (MIH) and insulin/insulin-like growth factor (IGF) peptides have received the most attention in lower vertebrates. The actions and receptor signaling pathways of these hormones often overlap and vary among species. The phosphatidylinositol-3 kinase (Pi3K) pathway, a primary signaling pathway for growth factors, is required not only for growth factor actions, but also for optimal gonadotropin and MIH induction of oocyte maturation in many fish species. The species-specific involvement of the Pi3K pathway in the integration of growth factor and MIH induction of ovarian follicle maturation and oocyte maturation is not well understood. Discovering the physiological basis for this variation will contribute to understanding the evolution of diverse reproductive strategies among fishes. Rainbow trout, (<em>Oncorhynchus mykiss</em>), appear unusual in that insulin and IGFs cannot induce resumption of meiosis or competence of the oocyte to respond to the MIH, 17,20βP, making it an interesting species to investigate Pi3K pathway signaling by MIH. In the present study, 17,20βP induction of meiosis resumption and Mapk activation were blocked or attenuated by pharmacological inhibition of Pi3K (wortmannin, LY294002) or Mek (PD98059, U0126), but were not affected by inhibitors of Akt (ML-9) or mTOR (rapamycin). The results indicate Pi3K and Mapk activation are required for optimal MIH-induction of meiosis resumption in rainbow trout follicles and may be required in other fishes in which insulin or IGFs do not directly induce competence or oocyte maturation.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102760"},"PeriodicalIF":3.2,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of dietary protein levels on growth, immune response, digestibility, intestinal microbiota, and transcriptome in Litopenaeus vannamei fed cottonseed protein concentrate","authors":"Hongming Wang ,&nbsp;Xin Hu ,&nbsp;Jian Chen ,&nbsp;Yudong Zheng ,&nbsp;Beiping Tan ,&nbsp;Lili Shi ,&nbsp;Shuang Zhang","doi":"10.1016/j.aqrep.2025.102762","DOIUrl":"10.1016/j.aqrep.2025.102762","url":null,"abstract":"<div><div>This study investigated the effects of dietary protein levels on <em>Litopenaeus vannamei</em> using cottonseed protein concentrate (CPC) as the primary protein source. <em>L. vannamei</em> (initial body weight = 0.42 ± 0.01 g) were fed diets containing 32 % (CPC32), 36 % (CPC36), 40 % (CPC40), 44 % (CPC44), and 48 % (CPC48) protein for 8 weeks. The CPC40 group exhibited the highest final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), and the lowest feed conversion ratio (FCR). Analysis of hepatopancreatic tissues revealed that activities of superoxide dismutase (SOD), peroxidase (PO), lysozyme (LZM), and catalase (CAT) were significantly reduced, while malondialdehyde (MDA) levels increased in the CPC48 group compared to the CPC40 group. The activities of trypsin and amylase were significantly decreased in the CPC32 and CPC48 groups compared to CPC40, with a notable decrease in trypsin activity also observed in the CPC36 group. Intestinal microbiota analysis showed significant shifts in the CPC40 group, including an increased Bacteroidetes to Proteobacteria ratio and higher relative abundances of Verrucomicrobia, Flavobacteriaceae, Demequinaceae, and <em>Photobacterium</em>. Transcriptomic analysis identified significant signaling pathways affected by dietary treatments, notably the IL-17 signaling pathway, endoplasmic reticulum protein processing, and cholesterol metabolism. Key genes such as <em>HSP90B1</em> and <em>HSP90AA1</em> were prominently involved in various pathways, including IL-17 signaling, thyroid hormone synthesis, fluid shear stress, prion disease, and atherosclerosis. Correlation analysis indicated a significant positive relationship between <em>HSP90B1</em> gene expression and <em>Photobacterium</em> abundance. Furthermore, gene expression levels of <em>HSP90B1</em> and <em>HSP90AA1</em> in hemocytes were significantly elevated in the CPC32 and CPC48 groups compared to CPC40. In conclusion, the CPC40 diet may enhance immune pathways in <em>L. vannamei</em> through the synergistic modulation of the <em>HSP90B1</em> gene and <em>Photobacterium</em>, leading to reduced intestinal pathogenic bacteria, improved immunity and digestive efficiency, ultimately promoting growth performance.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102762"},"PeriodicalIF":3.2,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of replacing wheat bran by palm kernel meal on growth performance and intestinal health of juvenile grass carp (Ctenopharyngodon idellus)","authors":"Wen Jiang ,&nbsp;Haifeng Mi ,&nbsp;Beiping Tan ,&nbsp;Shuang Zhang ,&nbsp;Shiwei Xie ,&nbsp;Lu Zhang ,&nbsp;Junming Deng","doi":"10.1016/j.aqrep.2025.102763","DOIUrl":"10.1016/j.aqrep.2025.102763","url":null,"abstract":"<div><div>Non-grain plant raw material palm meal can replace the limited grain raw material, which can provide a certain reference for aquaculture to reduce the cost and reduce the dependence on grain raw material. A 12-week feeding trial was conducted to evaluate the effects of substituting wheat bran with palm kernel meal (PKM) on the growth performance and intestinal health of grass carp (<em>Ctenopharyngodon idellus</em>). Six isoproteic (crude protein 32 %) and isolipidic (crude lipid 6 %) diets were formulated, incorporating varying levels of PKM replacing wheat bran: 0 % (PKM0), 20 % (PKM20), 40 % (PKM40), 60 % (PKM60), 80 % (PKM80), and 100 % (PKM100). Juvenile grass carp, with an initial body weight of 7.14 ± 0.04 g, were fed twice daily to satiation. The results indicated that PKM could completely replace wheat bran without compromising the growth performance of grass carp, with optimal growth observed at 40 % replacement level. High substitution levels (≥40 %) significantly increased intestinal trypsin and amylase activities, but decreased plasma contents of lipopolysaccharide, D-lactic acid, and endothelin-1. Partial substitution of wheat bran with PKM (20<img>40 %) significantly upregulated the relative expression of <em>zo-1</em> and <em>occludin</em> in midgut, whereas higher replacement levels (40<img>100 %) generally upregulated the relative expression of <em>tgf-1</em> and <em>il-10</em>. Similarly, high replacement levels (≥60 %) generally increased the contents of intestinal mucin 2, complement 4 and immunoglobulin M. In conclusion, the complete substitution of wheat bran with PKM did not adversely affect the growth performance of grass carp, with optimal growth occurring at 40 % replacement level. Additionally, high substitution levels (≥40 %) generally improved intestinal health by enhancing digestive enzyme activity, reducing intestinal mucosal permeability, and mitigating intestinal inflammatory responses.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102763"},"PeriodicalIF":3.2,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Archidendron clypearia extract: Modulating intestinal inflammation and enhancing disease resistance in aquacultured American bullfrogs (Aquarana catesbeiana)","authors":"Kesong Chen ,&nbsp;Han Lin ,&nbsp;Jingyang Sun ,&nbsp;Hua Li ,&nbsp;Jie Ma ,&nbsp;Zhendong Qin ,&nbsp;Biao Jiang ,&nbsp;Wei Li ,&nbsp;Qing Wang ,&nbsp;Youlu Su ,&nbsp;Li Lin ,&nbsp;Chun Liu","doi":"10.1016/j.aqrep.2025.102744","DOIUrl":"10.1016/j.aqrep.2025.102744","url":null,"abstract":"<div><div>Intestinal inflammation and bacterial infections challenges in the aquaculture of American bullfrogs (<em>Aquarana catesbeiana</em>), impacting their health and productivity. <em>Archidendron clypearia</em>, a traditional Chinese medicinal plant, is known for its analgesic, antibacterial, anti-inflammatory, and antioxidant properties, suggesting its potential as an integrative therapeutic agent in aquaculture. In this study, a short-term feeding experiment (35 days) was designed to explore the effects of <em>A. clypearia</em> extract (AE), on growth, intestinal morphology, antioxidant status, immune function and intestinal flora of bullfrog fingerlings. After feeding, bullfrogs were experimentally infected with <em>Elizabethkingia miricola</em> and monitored for an additional 14 days. Four groups were fed a commercial diet supplied with different AE as 0, 0.2, 1.0, and 5.0 g/kg. Results showed significant improvements in the growth and intestinal morphology of AE-supplied groups compared to the control. Moreover, Dietary supplemented with AE significantly increased expression levels of the tight junction protein–related genes, including zonula occludens-1(<em>ZO-1</em>), zonula occludens-2 (<em>ZO-2</em>), zonula occludens-3 (<em>ZO-3</em>), <em>occludin</em>, and <em>claudin-7</em>. However, the expression of inflammation-related genes, including Interleukin-8 (<em>IL-8</em>), Interleukin-1β (<em>IL-1β)</em>, C-X-C Motif Chemokine Ligand 10 (<em>CXCL10</em>), in the liver and intestine were significantly decreased in all AE groups compared to the controls (<em>P</em> &lt; 0.05). Alternatively, no significant impact on blood indices and intestinal microflora in all experimental groups. After challenge, the survival rates in 0, 0.2, 1.0 and 5.0 g/kg groups were 10 %, 50 %, 80 % and 70 %, respectively, suggesting enhancement of the bullfrog resistance in AE groups compared to control. The results suggest the AE positively impacted growth performance and disease resistance, making it a potentially useful functional feed additive in bullfrog breeding. The results suggest that adding 1.0 g/kg AE to the diet positively impacted growth performance and disease resistance, making it a potentially useful functional feed additive in bullfrog breeding.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102744"},"PeriodicalIF":3.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol mitigates the negative effects of feeding high-lipid diets to hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus)","authors":"Baiquan Lu ,&nbsp;Biao LI ,&nbsp;Menglong Zhou ,&nbsp;Yansheng Liu ,&nbsp;Hao Liu ,&nbsp;Weibin Huang ,&nbsp;Hengyang Song ,&nbsp;Wenshan Cai ,&nbsp;Beiping Tan ,&nbsp;Xiaohui Dong","doi":"10.1016/j.aqrep.2025.102747","DOIUrl":"10.1016/j.aqrep.2025.102747","url":null,"abstract":"<div><div>The objective of this experiment was to ascertain whether dietary supplementation with resveratrol (RES) could mitigate the negative effects of the high-lipid diet on hybrid grouper (♀ <em>Epinephelus fuscoguttatus</em> × ♂ E. <em>lanceolatus</em>). Four isonitrogenous (43 %) and isolipid (16 %) diets with 0 mg/kg, 100 mg/kg, 425 mg/kg and 1800 mg/kg RES were formulated respectively, which named R0, R1, R2 and R3, and administered to the grouper for a 42-day period. The results showed that hepatic somatic index (HSI) and whole-body crude lipid were significantly lower in the RES-treated groups, and visceral somatic index (VSI) was significantly lower in the R3 group than those in the control group (<em>P</em> &lt; 0.05). The addition of RES decreased serum levels of total cholesterol (TC) and low-density lipoprotein (LDL), and increased levels of triglyceride (TG) and high-density lipoprotein (HDL). Addition of moderate amounts of RES reduced hepatic TC, TG and very low-density lipoprotein (VLDL) content as well as fatty acid synthase (FAS) and acetyl coenzyme A carboxylase (ACC) activities, and increased lipoprotein lipase (LPL) and hepatic lipase (HL) activities. The number and area of hepatic lipid droplets were found to be significantly reduced in the RES-treated groups in comparison to the control group. RES significantly up-regulated the expression of peroxisome proliferator-activated receptor α (<em>pparα</em>) and lipoprotein lipase (<em>lpl</em>), and down-regulated the expression of peroxisome proliferator-activated receptor γ (<em>pparγ</em>) and fatty acid synthase (<em>fas</em>) (<em>P</em> &lt; 0.05). These results suggest that in high-lipid diets, feed RES improves lipid metabolism in grouper mainly through activation of lipolysis and inhibition of lipid synthesis. Under the conditions of this experiment, based on the analysis of the polynomial fitted curves for WGR and serum HDL content, it is recommended that 800–1000 mg/kg of RES be added to the high-lipid diet of juvenile hybrid grouper to improve lipid metabolism.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102747"},"PeriodicalIF":3.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global changes in gene expression of mRNA and miRNA in liver tissues of Micropterus salmoides after infection with Aeromonas hydrophila","authors":"Zhi Lei Yao ,&nbsp;Ming Yu ,&nbsp;Xiao Wang ,&nbsp;Hong Ju Chen ,&nbsp;Xiang Shan Ji ,&nbsp;Yan Zhao","doi":"10.1016/j.aqrep.2025.102756","DOIUrl":"10.1016/j.aqrep.2025.102756","url":null,"abstract":"<div><div><em>Aeromonas hydrophila</em> is a common pathogenic bacterium in cultivated Largemouth bass (<em>Micropterus salmoides</em>), but its pathogenicity to Largemouth bass and the molecular mechanism of host anti-<em>A. hydrophila</em> infection remains uncertain. In this study, Largemouth bass were infected with <em>A. hydrophila</em> to determine the median lethal dose (LD₅₀), histopathology and changes in gene expression. The results showed that the LD₅₀ was 2.8 × 10⁶ CFU/mL. The liver tissue of the diseased fish demonstrated enlarged parenchymal cells and vacuolated hepatocytes. Subsequently, liver tissues from healthy fish and those from infected with <em>A. hydrophila</em> were sampled separately for RNA sequencing. The results showed that a total of 2807 differentially expressed mRNAs (DE-mRNAs) and 42 differentially expressed miRNAs (DE-miRNAs) were obtained. Among them, 20 immune-related DE-mRNAs (<em>Raf1</em>, <em>Gcsfl</em>, <em>Traf6</em>, etc.) and 7 DE-miRNAs (miR-2188-x, miR-137-y, miR-144-y, etc.) were identified. Some immune-related pathways such as “AMPK signaling pathway” and “PPAR signaling pathway” were significant enrichment. A RT-qPCR of the randomly selected seven DE-mRNAs and seven DE-miRNAs confirmed the reliability of the RNA-seq. Additionally, integrative analysis of miRNA and mRNA identified a regulatory network consisting of 7 miRNAs and 18 mRNAs which were strong candidates for immune response. Furthermore, the dual-luciferase reporter assay confirmed that miR-144-y and miR-2188-x negatively regulate the expression of <em>Gcsfl</em> (granulocyte colony-stimulating factor-like), while miR-2188-x and miR-137-y negatively regulate the expression of <em>Traf6</em> (TNF receptor-associated factor 6), which plays central role in immune response in Largemouth bass after infection with <em>A. hydrophila</em>. Overall, our findings will provide new insights into the fundamental patterns of molecular responses induced by <em>A. hydrophila</em> in Largemouth bass.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102756"},"PeriodicalIF":3.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The physiological implications of dietary high-amylose starch on the growth performance, hepatic health indices, lipid metabolism, and intestinal microbiota composition in largemouth bass (Micropterus salmoides)","authors":"Han Huang ,&nbsp;Xianping Tian ,&nbsp;Hao Sun ,&nbsp;Xin Liu ,&nbsp;Yuanfa He ,&nbsp;Yongjun Chen ,&nbsp;Shimei Lin","doi":"10.1016/j.aqrep.2025.102752","DOIUrl":"10.1016/j.aqrep.2025.102752","url":null,"abstract":"<div><div>The present study aimed to explore the impacts of dietary high-amylose corn starch (HACS) as a substitute for conventional corn starch (CS)on the growth performance, hepatic health, lipid metabolism, and intestinal microbiota of largemouth bass. Five isonitrogenous and isolipidic feeds (47.90 % protein and 9.44 % lipid) were formulated with varying levels of HACS replacing CS at 0 % (HACS0), 2.5 % (HACS2.5), 5 % (HACS5), 7.5 % (HACS7.5), and 10 % (HACS10) for a 10-week growth trial with largemouth bass (initial body weight 10.02 g). The findings revealed that substituting CS with HACS in the feed elevated the final body weight, weight gain rate, and specific growth rate of largemouth bass. Additionally, the dietary replacement of CS with HACS tended to downregulate the hepatic mRNA expression levels of pro-apoptotic regulators (bcl-2 associated x a, apoptosis-related cysteine peptidase 3, and apoptosis-related cysteine peptidase 7) in largemouth bass. Histological examination of the liver indicated that the HACS2.5 group had a more intact and distinct hepatic structure than the HACS0 group. Moreover, dietary substitution of CS with HACS reduced serum triglyceride levels and liver lipid content, and there was a tendency to downregulate the mRNA expression of lipid synthesis metabolism-related genes (acetyl-CoA carboxylase 1, sterol regulatory element binding transcription factor 1, fatty acid synthase) in the liver. Concurrently, the HACS2.5 group upregulated the mRNA expression of lipolysis metabolism-related genes (carnitine palmitoyltransferase 1, peroxisome proliferator-activated receptor α, adipose triglyceride lipase) compared to the HACS0 group. Regarding the intestinal microbiota, the HACS2.5 group increased the abundance of <em>Fusobacteriota</em>, <em>Cetobacterium</em>, and <em>Cetobacterium somerae</em>, while decreased the relative abundance of <em>Proteobacteria</em> compared to the HACS0 group. Overall, dietary substitution of CS with HACS enhanced the growth performance of largemouth bass, potentially through the modulation of hepatic lipid metabolism equilibrium and the intestinal microbiota composition.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102752"},"PeriodicalIF":3.2,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitogenomic insights into the population structure and demographic history of zig-zag eels (Mastacembelus armatus) in southern China","authors":"Yuanyuan Wang ,&nbsp;Yexin Yang ,&nbsp;Yi Liu ,&nbsp;Yuli Wu ,&nbsp;Haiyan Liang ,&nbsp;Aiguo Zhou ,&nbsp;Fangyu Cui ,&nbsp;Jintao Peng ,&nbsp;Zhiyong Jiang ,&nbsp;Xidong Mu","doi":"10.1016/j.aqrep.2025.102748","DOIUrl":"10.1016/j.aqrep.2025.102748","url":null,"abstract":"<div><div>Understanding the genetic diversity and population structure of both wild and cultured fish populations is essential for developing effective conservation strategies and enhancing artificial breeding practices in aquaculture. <em>Mastacembelus armatus</em> (zig-zag eel) is an economically important fish species, yet some wild populations are facing declines, and large-scale artificial breeding remains challenging. To address this, we conducted a comprehensive analysis of genetic diversity and structure in wild and cultured populations. In this study, we sequenced and assembled the mitochondrial genomes of 98 wild and 90 cultured individuals. The results showed high genetic diversity in both wild and cultured populations and gene flow between them was detected. Significant genetic differentiation was observed among wild populations, primarily driven by river isolation. Conversely, genetic homogenization and low genetic differentiation were found within the Pearl River system. Phylogenetic analysis identified two main lineages, and demographic analyses revealed that lineage diversification occurred during the Pleistocene epoch, likely driven by habitat fragmentation caused by glacial retreats and fluctuating river systems. This study provides valuable genetic insights into the wild and cultured <em>M. armatus</em> populations in southern China, highlighting the need to preserve genetic differentiation in wild populations and addressing the genetic impact of escaped or released cultured individuals through improved management practices.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102748"},"PeriodicalIF":3.2,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}