Aquaculture Reports最新文献

{"title":"Establishment of two continuous adult stem cell lines from muscle and adipose tissues of rainbow trout (Oncorhynchus mykiss) for marbled fish meat production","authors":"Yaru Li ,&nbsp;Ting Xue ,&nbsp;Song Wang ,&nbsp;Yue Zhang ,&nbsp;Guoxiang Luan ,&nbsp;Jinwu Wang ,&nbsp;Huarong Guo","doi":"10.1016/j.aqrep.2025.102913","DOIUrl":"10.1016/j.aqrep.2025.102913","url":null,"abstract":"<div><div>Cell-cultured meat is an emerging technology with the potential to meet the nutritional needs, reduce environmental pollution and promote animal welfare. However, it is still a challenge to produce a marbled cell-cultured meat using the same species origin of muscle stem cells for protein production, and adipose-derived stem cells for fat production to enhance the texture, taste and tenderness. Here, we successfully established and characterized two continuous fish adult stem cell lines from rainbow trout (<em>Oncorhynchus mykiss</em>): muscle stem cell line (RTMS) and adipose-derived stem cell line (RTADS). These cell lines have maintained their myogenic/adipogenic potential over 50 and 30 times, respectively. Typically, muscle stem cells from fish species maintain their stemness for no more than 10 passages, while adipose-derived stem cells have not been previously established in fish. We optimized the differentiation media for RTMS cells, achieving differentiation and fusion indices of 35.89 % and 30.25 %, respectively, compared to the baseline medium which failed to induce differentiation. For RTADS cells, optimization of the differentiation medium significantly enhanced lipid droplet formation. Using 3D edible microcarriers, we produced marbled fat-containing cell-cultured fish meat of rainbow trout. The free amino acid and free fatty acid contents of the cell-cultured fish meat were found to be significantly higher than those of natural fish meat. This study provides two valuable seed cell lines for advancing cell-cultured fish meat production.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102913"},"PeriodicalIF":3.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144364954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance, physiological and immune responses of Nile tilapia Oreochromis niloticus fed extruded pellet diets with different binders","authors":"Eman Y. Mohammady ,&nbsp;Ümit Acar ,&nbsp;Elsayed M. Younis ,&nbsp;Abdel-Wahab A. Abdel-Warith ,&nbsp;Simon J. Davies ,&nbsp;Ehab R. El-Haroun ,&nbsp;Mohamed S. Hassaan","doi":"10.1016/j.aqrep.2025.102944","DOIUrl":"10.1016/j.aqrep.2025.102944","url":null,"abstract":"<div><div>The trial was conducted to investigate the effects of inclusion carboxymethyl cellulose (CMC) or calcium lignosulfonate (CLS) on the physical qualities of extruded Nile tilapia diet, growth performance, feed efficiency, physiological and immune response for 70-day. Three identical isonitrogenous and isoenergetic diet were formulated, 0 (control), 4 g CMC and 4 g CLS kg⁻¹ diet and fed to tilapia with an average initial body weight (14.20 ± 1.22 g). Inclusion of CLS significantly improved the water stability, durability, bulk density, and sinking speed of the pellets compared to CMC and control diets (P ≤ 0.05). Compared to the control group, inclusion of 4 g kg⁻¹ CLS recorded the highest weight gain (WG, 51.00 g fish⁻¹), specific growth rate (SGR, 3.02 %, day fish⁻¹) and aveage daily gain (ADG) (0.61 g fish⁻¹). The highest activities of amylase (84.102.44 U L⁻¹), lipase (958.3 ± 70.11 L) and trypsin (0.59 ± 0.18 ng ml⁻¹) were detected in fish fed CLS group. The height and width of villi and goblet cell number in both the anterior and posterior intestines were significantly increased in fish fed CLS than other groups. The activities of alanine amino transferase (ALT) and aspertate aminotransferase (AST) levels substantially decreased in the CLS group compared to the control. While CLS supplementation significantly elevated serum total protein, globulin, and albumin levels compared with CMC group and control diet. No significant differences were found in serum lipid profile among fish fed experimental diets. Catalase and superoxide dismutase (SOD) were significanly higher in fish fed diet supplemented with 4 g CLS kg⁻¹ compared with others group. Furthermore, immunoglobulin M (IgM) and complement factors (C3, C4), were significantly improved in fish fed diet contained CLS.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102944"},"PeriodicalIF":3.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the effects of anthropogenic EMF on fish embryo development: Survival and orientation responses","authors":"Jan Krzystolik,&nbsp;Adam Tański","doi":"10.1016/j.aqrep.2025.102953","DOIUrl":"10.1016/j.aqrep.2025.102953","url":null,"abstract":"<div><div>Research on the effects of electromagnetic fields (EMF) on living organisms, particularly developing fish embryos, is becoming increasingly important due to the rising number of electrical appliances and the expanding energy infrastructure. This article presents laboratory studies on the impact of anthropogenic EMF on the fertilized eggs of four fish species: pike, sea trout, vimba bream, and common chub. The study focused on the survival rates of developing eggs and the spatial orientation of embryos at the blastopore closure stage. The results reveal a differential effect of EMF on fish egg survival. Pike and sea trout eggs exhibited increased mortality when exposed to EMF, whereas vimba bream and common chub eggs showed no significant increase in mortality. The findings regarding embryo orientation under EMF exposure suggest possible changes in spatial orientation, although statistical analysis does not conclusively confirm this. Clear results were observed in the natural geomagnetic field of the Earth, with embryos showing a tendency to align along the north-south axis. In conclusion, although the study did not definitively confirm or exclude the influence of EMF on the survival and spatial orientation of embryos in the fish species studied, the findings provide important insights into the discussion about the impact of anthropogenic EMF on fish embryogenesis.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102953"},"PeriodicalIF":3.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144364843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Waterborne alkylphenol xenobiotic induces alteration of innate immunity, stress response, and immune/apoptotic related genes of Cyprinus carpio: Alleviation effect of Salvia officinalis","authors":"Abdallah Tageldein Mansour ,&nbsp;Afaf N. Abdel Rahman ,&nbsp;Motirh AL mutairy ,&nbsp;Fadwa M. Alkhulaifi ,&nbsp;Tarek Khamis ,&nbsp;Heba H. Mahboub ,&nbsp;Hassnaa Mahmoud Elsheshtawy","doi":"10.1016/j.aqrep.2025.102928","DOIUrl":"10.1016/j.aqrep.2025.102928","url":null,"abstract":"<div><div>The current perspective aimed to investigate the consequences of chronic exposure to alkylphenol xenobiotic (nonylphenol; NP) on humoral immune response, blood proteins, and stress indices as well as the immunological and pro/anti-apoptotic gene expression. In addition, this study assessed the potential ameliorative effect of common sage leaf powder (CSLP) against NP toxicity. A total of 240 mirror carp (<em>Cyprinus carpio</em> var <em>specularis</em>) were randomly distributed into four experimental groups with three replicates per group (20 fish/replicate) for 60 days: a control group, a group fed CSLP-supplemented diet (4 g/kg), a group exposed to NP (0.1 mg/L), and NP exposed group co-fed CSLP-supplemented diet. The exposure to NP at level 0.1 mg/L significantly (<em>P</em> &lt; 0.05) decreased serum immune-biochemical biomarkers (lysozyme activity, complement-3, total protein, albumin, and globulin levels), with a marked increase in stress indicators (glucose and cholesterol) levels. On the molecular level, exposure to NP at level 0.1 mg/L induced a down-regulation of immune-related gene expression (interleukin 1beta, toll-like receptor 7<em>,</em> interleukin 10, phosphoinositide-3-kinase regulatory subunit 3b(gamma), and nuclear factor kappa beta p65). The pro-apoptosis genes (Bcl-2 associated X-protein and cysteine-aspartic protease 3b) were boosted and the anti-apoptosis gene (B-cell lymphoma 2) was down-regulated by the exposure to NP. In addition, the expression of antioxidant (superoxide dismutase) and DNA repair (proliferating cell nuclear antigen) genes were disrupted. Conversely, dietary CSLP alleviated the alterations in the above-mentioned immune-biochemical and stress response parameters in the NP-exposed group. It also restored the disturbance of immune, antioxidant, and apoptosis regulatory-associated genes. In conclusion, NP could consider an immunotoxin and apoptotic inducer in <em>C. carpio</em> var <em>specularis</em>. The CSLP is a successful dietary supplement in aquafeed for its palliative effects against NP toxicity.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102928"},"PeriodicalIF":3.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144331181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptome analysis reveals pathogenesis of Procambarus clarkii during Black May Disease","authors":"Xueqi Teng,&nbsp;Chengwen Song,&nbsp;Yuxin Fan,&nbsp;Shiyu Jin,&nbsp;Si Luo","doi":"10.1016/j.aqrep.2025.102951","DOIUrl":"10.1016/j.aqrep.2025.102951","url":null,"abstract":"<div><div>Black May Disease (BMD) is a common illness that affects <em>Procambarus clarkii</em> during the spring-to-summer transition. With a complex pathogenesis, it poses a serious threat to aquaculture safety and industry stability. To better understand the progression of BMD, this study integrates key hepatopancreatic enzyme activity data with intestinal transcriptome profiles. We systematically investigated the immune, antioxidant, and metabolic adaptations of <em>P. clarkii</em> across different stages of the disease from both functional and molecular perspectives. The results showed a continuous increase in the relative abundance of <em>Aeromonas</em>, a typical opportunistic pathogen, throughout disease progression. This was accompanied by a gradual decline in the activities of key enzymes such as AKP, ACP, LPS, and SOD, indicating impaired nonspecific immune responses and basal metabolic functions. Several critical pathways—including PI3K-Akt, NOD-like receptor, Toll and Imd, AMPK, and glutathione metabolism—were significantly activated in the early disease stage (S2), sustained in the mid-stage (S3), and declined during the terminal stage (S4), reflecting a typical “early activation–mid-stage maintenance–late-stage decline” pattern. Pathways related to endocytosis, autophagy, and cell adhesion were consistently downregulated in the later stage, suggesting a systemic weakening of the intestinal barrier function. This study reveals a regulatory framework in which the accumulation of pathogens and external stress jointly disrupt the immune, metabolic, and structural defense systems. These findings offer theoretical insights and foundational data for understanding the pathogenesis of BMD and developing health management strategies for <em>P. clarkii</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102951"},"PeriodicalIF":3.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144331184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary mixtures of Bacillus spp. modulates intestinal morphology, resistance to Vibrio parahaemolyticus, response of immune-antioxidant genes, and growth of Dicentrarchus labrax","authors":"El-Sayed Hemdan Eissa ,&nbsp;Afaf N. Abdel Rahman ,&nbsp;Ragaa A. Ahmed ,&nbsp;Basma M. Hendam ,&nbsp;Yasmin M. Abd El-Aziz ,&nbsp;Hagar Sedeek Dighiesh ,&nbsp;Moaheda E.H. Eissa ,&nbsp;Ekemini Moses Okon ,&nbsp;Norhan H. Ahmed","doi":"10.1016/j.aqrep.2025.102930","DOIUrl":"10.1016/j.aqrep.2025.102930","url":null,"abstract":"&lt;div&gt;&lt;div&gt;Probiotics including &lt;em&gt;Bacillus&lt;/em&gt; spp. are gaining a lot of interest in fish diets due to their distinctive pharmacological and nutritional value. To look at the impact of dietary fortification of a mixture of &lt;em&gt;Bacillus&lt;/em&gt; spp. (MBs), a 56-day feeding trial was applied to European seabass (&lt;em&gt;Dicentrarchus labrax&lt;/em&gt;). The evaluation addressed the influence on growth, absorption, hematology, biochemical, and immune-antioxidant response including molecular level. The resistance to &lt;em&gt;Vibrio parahaemolyticus&lt;/em&gt; was also assessed. Five treatments having 225 fish (45 fish/treatment; three triplicates/treatment; 15 fish/replicate) were randomly assigned (8.00 ± 0.30 g body weight). The MBs were included in the basal diet at 0 (control), 1(MBs1), 2 (MBs2); 3 (MBs3); and 4 (MBs4) g/kg diet and given to the fish for 56 days. The results displayed that MBs-supplemented diets notably augmented growth (weight gain and specific growth rate) and intestinal morphology (villous length and width). The highest results for weight gain, specific growth rate, villous length and width were in the MBs3 (1.32-, 1.16-, 1.68-, and 1.45-fold) and MBs4 (1.33-, 1.17-, 1.78-, and 1.54-fold) groups, respectively, relative to the control. Dietary MBs (1–4 g/kg) significantly improved (&lt;em&gt;P&lt;/em&gt; &lt; 0.05) hematological [white blood cells count (1.12-fold), hematocrit (1.01-, 1.01-, 1.02-, and 1.04-fold), and hemoglobin (1.02-, 1.03-, 1.05-, and 1.06-fold)] and biochemical indices [albumin (1.05-, 1.08-, 1.11-, and 1.08-fold), globulin (1.13-, 1.24-, 1.46-, and 1.48-fold), total protein (1.09-, 1.16-, 1.29-, and 1.29-fold), and triglycerides (1.00-, 1.00-, 1.00-, and 1.01-fold)]. The antioxidant indices as catalase (1.15-, 1.15-, 1.16-, and 1.16-fold), superoxide dismutase (SOD; 1.06-, 1.09-, 1.10-, and 1.09-fold), and glutathione peroxidase (GPx; 1.02-, 1.10-, 1.11-, and 1.10-fold) were enhanced by dietary MBs (1–4 g/kg), respectively, compared with the control. The aspartate (0.98-, 0.98-, 0.97-, and 0.97-fold) and alanine (0.96-, 0.93-, 0.89-, and 0.89-fold) aminotransferases, alkaline phosphatase (0.97-fold), glucose (0.96-, 0.95-, 0.93-, and 0.93-fold), cholesterol (0.88-fold), and malondialdehyde (0.92-, 0.90-, 0.91-, and 0.91-fold) were declined by dietary MBs supplementation (1–4 g/kg), respectively. Moreover, the expression of growth [insulin-like growth factor 1 (1.41-, 1.91-, 2.15-, and 2.65-fold) and growth hormone (1.67-, 1.97-, 2.25-, and 2.72-fold)] and immuno-antioxidant associated genes [complement3 (1.42-, 1.95-, 2.33-, and 2.52-fold), interleukin-1 beta (1.30-, 1.76-, 2.43, and 2.82-fold), &lt;em&gt;SOD&lt;/em&gt; (1.40-, 1.86-, 2.47-, and 2.64-fold), and &lt;em&gt;GPx&lt;/em&gt; (1.22-, 1.75-, 2.55-, and 2.75-fold)] were up-regulated in MBs-fed groups (1–4 g/kg), respectively. Post-challenge with &lt;em&gt;V. parahaemolyticus&lt;/em&gt;, the MBs3 and MBs4 groups had the highest survival rate (70.00 and 70.00 %), followed by the MBs2 group (60.00 %) and MBs1 group (50.","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102930"},"PeriodicalIF":3.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144331183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early sensory responses to plant-based diets in rainbow trout (Oncorhynchus mykiss) alevins: Impact on feeding behavior","authors":"Maud Martinat,&nbsp;Marie Lasserre,&nbsp;Elodie Baranek,&nbsp;Anne Surget,&nbsp;Anthony Lanuque,&nbsp;Frédéric Terrier,&nbsp;Cécile Heraud,&nbsp;Jérôme Roy","doi":"10.1016/j.aqrep.2025.102943","DOIUrl":"10.1016/j.aqrep.2025.102943","url":null,"abstract":"<div><div>Smell and taste are key sensory modalities involved in nutrient detection and feeding behavior in fish. In rainbow trout (<em>Oncorhynchus mykiss</em>), early growth can be compromised when fishmeal and fish oil are replaced by plant-based ingredients. However, the sensory mechanisms involved in feed detection at the alevin stage remain poorly understood. This study aimed to investigate how plant-based diets affect gustatory and olfactory responses during early development, and how these changes relate to feeding behavior. A postprandial kinetic experiment was conducted from first feeding to 30 days, using two diets: a commercial-like diet (C), composed of fishmeal, fish oil, and plant ingredients, and a fully plant-based diet (V). Fish were either fasted or sampled at 20 min, 2 h, 6 h, 10 h, and 24 h post-feeding. We measured the expression of genes involved in nutrient sensing (fatty acids, amino acids, sugars), taste modalities (sweet, salty, sour, acidic, bitter, umami), and calcium signaling in the olfactory rosette and tongue. We also analyzed neurotransmitter pathways (serotonin and dopamine), appetite-regulating neuropeptides in the brain, and intestinal hormones (CCK, ghrelin). Results showed that fish fed the V diet had significantly reduced growth performance (p &lt; 0.01) and feed intake after 30 days (p &lt; 0.01), associated with altered sensory gene expression. In C-fed fish, nutrient and taste receptors were mainly upregulated in the tongue, while in V-fed fish, expression shifted toward the olfactory rosette. C-fed fish also showed distinct calcium signaling patterns in the tongue and higher serotonin turnover. Expression of melanocortin 4 receptors (<em>mc4ra, mc4rb</em>) was elevated in V-fed fish (p &lt; 0.05), suggesting central anorexigenic signaling. Together, these results indicate that V diets disrupt early feeding responses through changes in sensory perception and neuroendocrine regulation. This highlights the importance of considering sensory cues when formulating sustainable feeds for aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102943"},"PeriodicalIF":3.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144331182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lycopene alleviates high carbohydrate diet induced hepatic steatosis in black seabream (Acanthopagrus schlegelii): Potential for nutritional intervention","authors":"Xinyan Zhi ,&nbsp;Yixin Gu ,&nbsp;Yangguang Bao ,&nbsp;Wenli Zhao ,&nbsp;Xuran Liu ,&nbsp;Jiayun Zhu ,&nbsp;Hao Cheng ,&nbsp;Dingqi Mao ,&nbsp;Tingting Zhu ,&nbsp;Peng Sun ,&nbsp;Qicun Zhou ,&nbsp;Min Jin","doi":"10.1016/j.aqrep.2025.102940","DOIUrl":"10.1016/j.aqrep.2025.102940","url":null,"abstract":"<div><div>Lycopene (Lyc), a plant-derived carotenoid known for regulating glucose and lipid metabolism, which are used to investigate the effects of dietary Lyc supplementation on juvenile black seabream (<em>Acanthopagrus schlegelii</em>) (initial weight, 2.20 ± 0.02 g). A ten-week feeding trial was performed by employing four experimental diets: a regular carbohydrate diet (Control, 22.81 % nitrogen-free extract, NFE); a high-carbohydrate diet (HCD, 32.71 % NFE), and two HCD diets supplemented with 24.50 mg/kg (HCD + L1) or 48.80 mg/kg (HCD + L2) of Lyc. Results showed that dietary Lyc supplementation improved glucose tolerance and insulin resistance, while significantly reducing liver glycogen content and lipid droplet accumulation. In contrast, muscle glycogen content was upregulated compared to the HCD group. At the molecular level, Lyc supplementation activated the <em>ins</em>/<em>pi3k</em>/<em>akt</em> pathway, contributing to a dramatic up-regulation in the transcription of glucose transporters (<em>glut2</em>), along with key glycolytic genes (<em>gk</em> and <em>pfk</em>). Conversely, the transcription of gluconeogenesis-related genes (<em>pepck</em> and <em>g6pc</em>) was downregulated. Moreover, Lyc supplementation activated the <em>ampkα</em>/<em>sitrt1</em> pathway, generating a considerable lessening in the transcription of lipid biosynthesis genes (<em>srebp-1c</em>, <em>fas</em>, <em>accα</em>, and <em>g6pd</em>). Alternatively, the transcription of lipolysis-related genes (<em>pparα</em>, <em>acox3</em>, and <em>atgl</em>) were upregulated, promoting hepatic lipid depletion. Correspondingly, TG and LDL-c levels, as well as ALT and AST activities, were reduced. In conclusion, dietary Lyc supplementation alleviated liver glycogen accumulation and lipid deposition induced by HCD. These findings suggest that Lyc effectively improves glucose and lipid metabolism, offering therapeutic potential for metabolic disorders in <em>A. schlegelii</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102940"},"PeriodicalIF":3.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144331185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of fishmeal replacement with peanut meal on growth, antioxidant capacity, digestive enzyme activity and protein metabolism-related gene expression in swimming crab Portunus trituberculatus","authors":"Xishuai Cui ,&nbsp;Hongyu Peng ,&nbsp;Tingting Zhu ,&nbsp;Yao Deng ,&nbsp;Shichao Xie ,&nbsp;Wenhao Zhan ,&nbsp;Peng Sun ,&nbsp;Tiantian Xu ,&nbsp;Haiqing Cao ,&nbsp;Zheng Tang ,&nbsp;Min Jin ,&nbsp;Qicun Zhou","doi":"10.1016/j.aqrep.2025.102936","DOIUrl":"10.1016/j.aqrep.2025.102936","url":null,"abstract":"<div><div>To assess the effects of fishmeal (FM) replacement with peanut meal (PM) on growth performance, antioxidative capacity and the expression of genes associated with protein metabolism and inflammation in <em>Portunus trituberculatus</em>, a 56-day feeding trial was conducted. The basal diet (PM0) comprised 30 % FM, while the experimental diets were formulated by replacing fishmeal with peanut meal at 10 % (PM10), 20 % (PM20), 30 % (PM30), and 40 % (PM40) substitution levels, respectively. The results revealed that 30 % and 40 % FM replacement with PM markedly reduced PWG. Crabs fed PM30 and PM40 diets exhibited significantly lower activities of pepsin and trypsin in hepatopancreas compared to other treatments, while the highest Na⁺, K⁺-ATPase activity was recorded in crabs fed diet PM20. Crabs fed PM20 diet showed higher T-SOD, T-AOC and nitric oxide activities in the hemolymph than those fed others. The expression of <em>4e-bp1</em>, a gene associated with the TOR signaling pathway, was markedly up-regulated in the hepatopancreas of crabs fed PM30 and PM40 diets, whereas <em>tor</em> expression in muscle was down-regulated with increasing FM replacement. Furthermore, <em>gpx</em> and <em>cat</em> expression in the hepatopancreas was markedly down-regulated, while <em>myd88</em> and <em>tlr4</em> expression in the intestine was up-regulated in crabs fed 30 % and 40 % replacement diets. In summary, the optimal replacement level of fishmeal (FM) with peanut meal (PM) was 20.05 %, while replacements exceeding 30 % suppressed growth and impaired protein metabolism.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102936"},"PeriodicalIF":3.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Replacing fishmeal with an insect meal blend: Implications for intestinal microbiota in European seabass","authors":"Violeta Kalemi ,&nbsp;Simona Rimoldi ,&nbsp;Rafaela S. Costa ,&nbsp;Ana Basto ,&nbsp;Marta Monteiro ,&nbsp;Genciana Terova ,&nbsp;Luisa M.P. Valente","doi":"10.1016/j.aqrep.2025.102939","DOIUrl":"10.1016/j.aqrep.2025.102939","url":null,"abstract":"<div><div>In this study, we investigated the effects of an insect meal (IM) blend containing larvae of <em>Hermetia illucens</em> and <em>Tenebrio molitor</em> on the gut microbiota of European seabass (<em>Dicentrarchus labrax</em>), a key species in Mediterranean marine aquaculture. This research stands out by examining a combination of IMs rather than a single source, thereby addressing an underexplored area in the current literature. In a feeding trial that lasted 75 days, European seabass were fed three different diets: a commercial-like control diet and two experimental diets containing partial replacement of FM with IM (25 % and 50 %). The experimental diets showed no adverse effects on feed intake or overall fish health. Analysis of the microbiota revealed significant changes in gut microbial communities, with a trend towards increased bacterial richness and diversity in fish fed IM. Beta diversity analysis showed that the mucosa-associated microbial communities were stable across diets, while the digesta-associated microbiota showed notable shifts in the IM25 and IM50 groups, suggesting that the transient microbiota is more sensitive to dietary changes. The study also found an enrichment of beneficial bacterial genera, particularly <em>Bacillus</em> and <em>Paenibacillus</em>, in fish fed IM. These genera, known for their chitinolytic activity, have likely adapted to the increased chitin content in IM diets. <em>Oceanobacillus</em> (<em>Bacillaceae</em>) and <em>Brevibacterium</em> (<em>Brevibacteriaceae</em>) were more abundant in the digesta of fish from the IM25 and IM50 groups, but not in the mucosa. Their presence indicates that they react more strongly to changes in diet than to a stable mucosal environment. Overall, the study highlights the potential of an IM-based diet to support fish health and growth while promoting a favorable gut microbiota.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102939"},"PeriodicalIF":3.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144331180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}