Aquaculture Reports最新文献

{"title":"Exploring the growth performance, antioxidant capacity, and metabolism of Babylonia areolata in response to yeast selenium supplementation","authors":"Yunting Wang ,&nbsp;Waqas Ahmed ,&nbsp;Sajid Mehmood ,&nbsp;Mohsin Mahmood ,&nbsp;Lu Wang ,&nbsp;Wenjie Ou ,&nbsp;Jiannan Li ,&nbsp;Jie Yang ,&nbsp;Weidong Li ,&nbsp;Lianshun Wang","doi":"10.1016/j.aqrep.2025.102617","DOIUrl":"10.1016/j.aqrep.2025.102617","url":null,"abstract":"<div><div>This study aimed to examine the effects of yeast selenium on growth, antioxidant enzymes, and metabolites activity in <em>Babylonia areolata</em> under different selenium concentrations. Except control group, the experiment involved five other diets, with one containing sodium selenite (1.6 mg Se/kg diet) and three containing graded levels of yeast selenium (L-YSe (2.4 mg Se/kg diet), M-YSe (14.4 mg Se/kg diet), H-YSe (22.5 mg/kg), and one containing yeast (2 g/kg). The <em>B. areolata</em> (initial weight: 7.7 ± 0.9 g) were divided into four groups and fed with the different selenium diets. The results indicated that weight gain and survival rates of the M-YSe group (17.59 % and 100 %, respectively) were significantly higher than the CK (13.37 % and 96.67 %, respectively) (p = 0.0001 and p = 0.01, respectively). The hepatopancreas catalase (CAT) and glutathione peroxidases (GSH-Px) levels increased with the increasing dietary selenium content. The SOD, CAT, T-AOC levels in the M-YSe group were significantly increased by (34.05 %, 151.62 % and 131.5 %, respectively) than in the CK groups, while the MDA concentration was significantly decreased by (66.67 % lower). Additionally, our results suggest that yeast selenium supplementation activated related metabolites and glutamine metabolic pathways in <em>B. areolata</em>. These findings provide insights into the nutritional and health benefits of selenium-enriched <em>B. areolata</em>. The results also evidence that <em>B. areolata</em> has the potential to be used as a functional food source that can benefit human health due to its high selenium content. Thus, this study provides a theoretical basis for further exploration of the edible value and health care functions of <em>B. areolata</em>-enriched selenium.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102617"},"PeriodicalIF":3.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncMPEG1/miR-2c regulates exoskeleton biocalcification via engrailed in bivalve","authors":"Minxin Liang ,&nbsp;Caixia Cai ,&nbsp;Ziqi Ren ,&nbsp;Zhiqing Li ,&nbsp;Ronglian Huang ,&nbsp;Qingheng Wang ,&nbsp;Yongshan Liao ,&nbsp;Chuangye Yang ,&nbsp;Zhe Zheng","doi":"10.1016/j.aqrep.2025.102616","DOIUrl":"10.1016/j.aqrep.2025.102616","url":null,"abstract":"<div><div>LncRNAs and miRNAs has been reported to play important regulation roles in skeleton growth<strong>.</strong> This research elucidates the molecular interplay between long noncoding RNA <em>LncMPEG1</em> and miRNA <em>miR-2c</em> in the biocalcification process of shell formation. The study establishes <em>LncMPEG1</em> as a molecular sponge for <em>miR-2c</em>, modulating the expression of key biomineralization genes, including homeobox protein engrailed and matrix metalloproteases (MMPs). Utilizing miRanda prediction, interaction sites between <em>miR-2c</em> and <em>LncMPEG1</em> were identified and validated through RNA immunoprecipitation and luciferase assays. In vivo overexpression of <em>miR-2c</em> suppressed <em>engrailed</em> and <em>MMP</em> expression, while <em>miR-2c</em> inhibition induced the opposite effect. Luciferase assays confirmed miR-2c's inhibitory effect on the 3′UTR of target genes (p &lt; 0.001). Western blot analysis corroborated these findings, showing miR-2c's regulatory impact on protein expression levels. <em>LncMPEG1</em> interference significantly reduced target gene transcription (p &lt; 0.01), and its overexpression enhanced the reporter vector's activity by binding <em>miR-2c</em> (p &lt; 0.05). Collectively, these results underscore LncMPEG1's role in shell formation through competitive binding with <em>miR-2c</em>, thereby regulating the expression of biomineralization-related genes.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102616"},"PeriodicalIF":3.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotype-environment interactions on growth and inner shell color variation in Hyriopsis cumingii cultured in different iron-level substrates","authors":"Xuefeng Lv ,&nbsp;Zhiyan Wang ,&nbsp;Quanzhi Bao ,&nbsp;Changzhi Li ,&nbsp;Tingting Lu ,&nbsp;Yuyang Wu ,&nbsp;Chuntong Liu ,&nbsp;Zhiyi Bai","doi":"10.1016/j.aqrep.2025.102621","DOIUrl":"10.1016/j.aqrep.2025.102621","url":null,"abstract":"<div><div><em>Hyriopsis cumingii</em> is a species that naturally inhabits substrates rich in various metal elements. Iron, essential for several biological processes, significantly influences the growth and development of bivalves. This study established nine full-sibling families and conducted an 11-month cultivation experiment involving five different levels of iron content in substrates (1.75 %, 3.85 %, 4.75 %, 5.95 %, and 6.85 %) to investigate the impact of varying iron concentrations on the growth and inner shell color of <em>H. cumingii</em>. The findings revealed that <em>H. cumingii</em> demonstrated significantly enhanced growth under the Iron 3 condition (4.75 %), while individuals cultivated under Iron 4 (5.95 %) exhibited a deeper purple pigmentation in their inner shell. Heritability estimates for growth and inner shell color traits ranged from 0.07 to 0.25 across the experimental population. The anal angle radial rib length (AARRL) displayed stronger genetic and phenotypic correlations with body weight (BW) and shell width (SW) compared to shell height (SH) and total shell height (TSH). Notably, significant gene-environment interaction effects (G × E) were notably observed for the color exhibited by the inner shell and growth-related traits, especially between Iron 1 and Iron 3, as well as between Iron 3 and Iron 5. The comprehensive estimation of breeding values showed that Family 7 exhibited superior performance across all traits under different conditions, positioning it as a promising candidate for genetic improvement. This research underscores the considerable influence of iron concentrations in substrates on the growth and shell coloration of <em>H. cumingii</em>, offering valuable insights for developing selective breeding strategies.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102621"},"PeriodicalIF":3.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The semi-wild mariculture of Caribbean King Crab (Maguimithrax spinosissimus) for coral reef restoration","authors":"Samantha Glover ,&nbsp;Mark J. Butler IV","doi":"10.1016/j.aqrep.2024.102612","DOIUrl":"10.1016/j.aqrep.2024.102612","url":null,"abstract":"<div><div>Aquaculture has traditionally focused on supporting human food production, but there is a growing need for aquaculture associated with environmental restoration. For example, the <em>in situ</em> and <em>ex situ</em> rearing of corals for coral reef restoration is now implemented worldwide, but its success is limited by the lack of reef herbivores to combat the phase shift from coral-to-macroalgae-dominance on reefs, especially in the Caribbean. Laboratory-based aquaculture of some reef herbivores is possible but labor-intensive and insufficient to supply the numbers needed for large-scale restoration. We examined whether this bottleneck could be alleviated by the semi-wild mariculture of the Caribbean King Crab (<em>MaguiMithrax spinosissimus</em>)in saltwater quarries for subsequent use in coral reef restoration in the Florida Keys, Florida (USA).We surveyed the physico-chemical characteristics of 16 saltwater quarries in the Florida Keys relative to conditions in the wild and, in four quarries where crabs occurred, we examined crab population dynamics in comparison to that in the wild. Water chemistry and the availability of food (macroalgae) and crevice shelters were suitable for crab mariculture down to depths of at least 6 m. Herbivore competitors and predators were few in quarries, and there was no difference in the population size structure, fecundity, predatory mortality, or health of crabs in quarries compared to that in the wild. Therefore, the low-cost semi-wild mariculture of large numbers <em>M. spinosissimus</em> is feasible in saltwater quarries and could alleviate a logistical bottleneck to herbivore restoration on Caribbean coral reefs.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102612"},"PeriodicalIF":3.2,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulatory effects of selenium nanoparticles on gut microbiota and metabolites of juvenile Nile tilapia (Oreochromis niloticus) by microbiome-metabolomic analysis","authors":"Jing Ni ,&nbsp;Lirong Ren ,&nbsp;Ying Liang ,&nbsp;Ying Ma ,&nbsp;Hejian Xiong","doi":"10.1016/j.aqrep.2025.102627","DOIUrl":"10.1016/j.aqrep.2025.102627","url":null,"abstract":"<div><div>Selenium nanoparticles (SeNPs) are considered safe selenium supplements. At appropriate concentrations, SeNPs can promote growth, boost immunity, and modulate intestinal microbiota in aquatic animals. However, their effects on the metabolome of aquatic species have not yet been identified. This study evaluated the <em>in vitro</em> effects of our previous synthesized SeNPs, coated with abalone visceral polysaccharide-protein complexes (PSP-SeNPs), on the proliferation of probiotics and common aquatic pathogens, and the <em>in vivo</em> effects on the gut microbiome and metabolome of tilapia. PSP-SeNPs selectively promoted the growth of probiotics <em>Lactobacillus rhamnosus</em> and <em>Lactococcus lactis</em>, while inhibiting the growth of pathogens such as <em>Staphylococcus aureus</em> and <em>Aeromonas hydrophila.</em> In the fish breeding experiment, 360 healthy juvenile tilapias (initial weight 1.5 ± 0.5 g/fish) were divided into four groups (3 replicates/group, 30 fish/replicate): group C was fed with basal diet, groups Y, M and H, were fed with the basal diet supplemented with 0.3 mg/kg of Na₂SeO₃, 0.3 mg/kg of PSP-SeNPs, and 4.5 mg/kg of PSP-SeNPs, respectively. All fish were tested after 7 weeks of breeding in a recirculating aquaculture system. Results showed that group M significantly increased intestinal microbial diversity and potential probiotics (e.g., <em>Cetobacterium</em>, <em>Fimbriiglobus</em>, and <em>Gemmata</em>), while significantly decreased potential pathogens (<em>Plesiomonas</em>, <em>Citrobacter freundii</em>, and <em>Aeromonas hydrophila</em>). Groups H and Y significantly reduced <em>Citrobacter freundii</em>, but both groups decreased gut microbial diversity to varying degrees. Additionally, the pathogenic bacterial genus <em>Plesiomonas</em> significantly increased in group Y. In group M, the growth-promoting intestinal metabolite alpha-tocotrienol was significantly upregulated, whereas some essential amino acids were significantly downregulated in groups Y and H. In summary, 0.3 mg/kg SeNPs supplementation can regulate the intestinal microbiota, while 4.5 mg/kg SeNPs and 0.3 mg/kg Na₂SeO₃ can cause amino acid metabolism disorders. SeNPs showed higher safety than Na₂SeO₃.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102627"},"PeriodicalIF":3.2,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of all-triploid derived from gynogenetic homologous diploid crucian carp (♀) × diploid red crucian carp (♂)","authors":"Chongqing Wang ,&nbsp;Yan Tang ,&nbsp;Xiang Luo ,&nbsp;Yuxin Zhang ,&nbsp;Yue Zhou ,&nbsp;Qingwen Xiao ,&nbsp;Zhengkun Liu ,&nbsp;Xinyi Deng ,&nbsp;Xu Huang ,&nbsp;Xidan Xu ,&nbsp;Xiaowei Xu ,&nbsp;Kun Zhang ,&nbsp;Qinbo Qin ,&nbsp;Shaojun Liu","doi":"10.1016/j.aqrep.2025.102620","DOIUrl":"10.1016/j.aqrep.2025.102620","url":null,"abstract":"<div><div>Triploid breeding is vital in aquaculture production. Here, based on the formation of all-female gynogenetic homologous diploid crucian carp (G, 2 n = 100) derived from gynogenesis of the autotetraploid crucian carp (4nRR, 4 n = 200) eggs and without chromosome duplication treatment, a novel triploid fish (3nRR, 3 n = 150) were produced by crossing G and males of red crucian carp (RCC, 2 n = 100). All 3nRR possessed 150 chromosomes and were triploid hybrids. The genetic characteristics of 5S rDNA in 3nRR individuals and their parental counterparts, as well as the measurable and countable traits, were explored. The 3nRR males exhibited improper sperm development, whereas 3nRR females produced mature eggs by two years old. Females of 3nRR produced various-sized eggs that fertilized with haploid sperm from RCC, producing viable diploids, triploids and tetraploids. Analysis of gonadal transcriptome between 3nRR and RCC revealed that <em>BMP2</em> was linked to fertility of female 3nRR, and <em>SOX2</em> plays a significant role in the male sterility of 3nRR. The findings of this study suggested that the stable production of triploid fish could be achieved via the hybridization of diploid fish. The triploids have important application values in protecting germplasm resources and preparing polyploid fish.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102620"},"PeriodicalIF":3.2,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pumpkin seed oil-loaded chitosan nanoparticles enhance growth, digestive enzymes, and immune response in whiteleg shrimp (Litopenaeus vannamei): Impacts on histopathology and survival against Vibrio parahaemolyticus","authors":"El-Sayed Hemdan Eissa ,&nbsp;Wesam Mohamed Salama ,&nbsp;Samia Elbahnaswy ,&nbsp;Mai A.M. El-Son ,&nbsp;Zienab E. Eldin ,&nbsp;Sara Amer ,&nbsp;Layla A. Almutairi ,&nbsp;Fatma Mohamed Ameen Khalil ,&nbsp;Safia M.A. Bahshwan ,&nbsp;Mada M. AL-Qurashi ,&nbsp;Yasmin M. Abd El-Aziz ,&nbsp;Moaheda E.H. Eissa ,&nbsp;Aiman A. Alsaegh ,&nbsp;Roshmon Thomas Mathew ,&nbsp;El Sayed Taha Rizk","doi":"10.1016/j.aqrep.2024.102599","DOIUrl":"10.1016/j.aqrep.2024.102599","url":null,"abstract":"<div><div>The research evaluated the innovative effects of pumpkin seed oil-loaded chitosan nanoparticles (ChNPs/PSO) on the growth, body composition, digestive enzymes, immune response, organ histopathology, and survivability of whiteleg shrimp (<em>Litopenaeus vannamei</em>) juveniles against <em>Vibrio parahaemolyticus</em>. Shrimp were fed basal diets (control diets and 1 g of free nano chitosan; ChNPs) supplemented with ChNPs and different levels of ChNPs/PSO at 1 (ChNPs+PSO₁), 1.5 (ChNPs+PSO_1.5), and 2 (ChNPs+PSO₂) mL/kg for a continuous 60 days period. At the end of the feeding trial, shrimp juveniles were exposed to <em>V. parahaemolyticus</em>. The results showed that dietary inclusion of ChNPs/PSO (1.5 or 2 mL) significantly improved the final weight, feed conversion ratio, weight gain, and specific growth rate of shrimp compared to other groups (P &lt; 0.05). Shrimp fed diets supplemented with 1.5 or 2 mL of ChNPs/PSO demonstrated significantly higher survival rates compared to other groups. These groups also exhibited significant increases in crude protein, ash content, phagocytic index, superoxide dismutase, and lipase levels (P &lt; 0.05). The ChNPs+PSO₂ group showed the highest amylase activity (P &lt; 0.05). Furthermore, shrimp in these groups displayed significantly elevated total hemocyte counts, lysozyme activity, and bactericidal capacity (P &lt; 0.05). Besides, a significant increase in phenoloxidase and phagocytic activities was observed in the ChNPs+PSO₁ group (P &lt; 0.05). The expression levels of prophenoloxidase (<em>LvproPO</em>) and hemocyanin (<em>LvHc</em>) genes were significantly increased in the hepatopancreas of shrimp fed diets enriched with 1.5 or 2 mL of ChNPs/PSO. The survival rate after infection with <em>V. parahaemolyticus</em> was higher in all ChNPs/PSO-enriched groups compared to the control group. Dietary supplementation with ChNPs/PSO restored the histological damages in the muscle, hepatopancreas, and intestine induced by <em>V. parahaemolyticus</em> infection. In summary, pumpkin seed oil-loaded chitosan nanoparticles (ChNPs/PSO) hold potential as a feed additive for enhancing growth performance, immune ability, and disease resistance against <em>V. parahaemolyticus</em> in Whiteleg shrimp.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102599"},"PeriodicalIF":3.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary Spirulina platensis supplementation on growth performance, whole body composition, antioxidant activity, histological alterations, and resistance to Vibrio parahaemolyticus in Pacific white shrimp, Litopenaeus vannamei","authors":"Ragaa A. Ahmed ,&nbsp;Samyah D. Jastaniah ,&nbsp;Bothaina A. Alaidaroos ,&nbsp;Manal E. Shafi ,&nbsp;Ehab El-Haroun ,&nbsp;Yasmin M. Abd El-Aziz ,&nbsp;Ola Hasan Abd El Megeed ,&nbsp;Mada M. AL-Qurashi ,&nbsp;Safia M.A. Bahshwan ,&nbsp;Mohammad Bodrul Munir ,&nbsp;Zulhisyam Abdul Kari ,&nbsp;Roshmon Thomas Mathew ,&nbsp;Moaheda E.H. Eissa ,&nbsp;El-Sayed Hemdan Eissa ,&nbsp;Amal Elfeky","doi":"10.1016/j.aqrep.2024.102606","DOIUrl":"10.1016/j.aqrep.2024.102606","url":null,"abstract":"<div><div><em>Spirulina platensis</em> has been extensively studied from its economic significance. It is also used as a feed additive in aquafeeds, where it serves as a growth promoter and immune stimulant. The genus <em>S. platensis</em> recognize for its high protein content, carotenoid, phycocyanin and phytochemicals content, which can help mitigate challenges in the aquaculture industry challenges such as disease outbreaks, pathogen infection and ultimately contributing to aquaculture sustainability. In this context, an eight-week feeding experiment was conducted to investigate the potential effects of dietary <em>S. platensis</em> (SP) on feed utilization, growth performance, antioxidant activity, and muscle and hepatopancreatic histology of <em>Litopenaeus vannamei</em> challenged with <em>Vibrio parahaemolyticus</em>. A total of 300 shrimps were included in this experiment. Shrimp were fed diets enriched with 2 g/kg (T1), 4 g/kg (T2), 6 g/kg (T3), and 8 g/kg (T4) of SP with the control group (T0) being fed a basal diet. The results showed that shrimp fed diets supplemented with 4, 6, or 8 g/kg SP exhibited significant improvements in the final body weight, weight gain, specific growth rate, feed conversion ratio, and survival rate (P ≤ 0.05). Shrimp in the T3 and T4 groups exhibited significantly lower malondialdehyde activity (P ≤ 0.05) compared to the other groups. All SP-supplemented groups resulted in higher levels of GPx, SOD, and CAT compared to the T0 group (P &lt; 0.05). Feeding shrimp diets supplemented with 8 g of SP resulted in the highest crude protein and ash content, with significantly lower crude lipid levels in the whole body of shrimp (P &lt; 0.05). Shrimp fed 8 g/kg of SP showed the highest survival rate (P ≤ 0.05) against <em>V. parahaemolyticus</em>. Including SP in shrimp diets, especially at 6 and 8 g/kg levels, improved the histological profile of muscle and hepatopancreatic tissues. Overall, the present study highlights the potential impact of <em>S. platensis</em> in shrimp diet by showing significant improvements in growth, antioxidant capacity, histological changes and resistance to against <em>V. parahaemolyticus</em> Thus, <em>S. platensis</em> can be recommended as a beneficial feed supplement for the shrimp industry.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102606"},"PeriodicalIF":3.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary bile acid on the growth performance, intestinal health, blood biochemistry, and antioxidative response of Nile tilapia (Oreochromus niloticus) fed high-fat diets","authors":"Mahmoud A.O. Dawood ,&nbsp;Sameh El-Dahan ,&nbsp;Samy Elsaadawy ,&nbsp;Ahmed E. Noreldin ,&nbsp;Hani Sewilam","doi":"10.1016/j.aqrep.2024.102605","DOIUrl":"10.1016/j.aqrep.2024.102605","url":null,"abstract":"<div><div>High-fat (HF) diets are often used in aquaculture as a nonprotein energy source, resulting in lower feed costs and nitrogen emissions. However, high-fat diets have a deleterious influence on aquatic species' metabolic and immunological functioning. Thereby, bile acid (BA) is proposed to relieve the lipid metabolism dysfunction, oxidative stress, and malnutrition caused by HF. In this study, Nile tilapia (4.97 ± 0.34 g) were distributed in six groups in triplicates and stocked in fifteen 100 L plastic tanks. Fish offered six isonitrogenous test diets for 60 days: the control (6 % fat) without BA (LF) and five HF (12 % fat) diets supplemented with BA at 0, 0.05, 0.1, 0.15, and 0.2 g/kg. The results indicated that the HF diet lowered growth, feed efficiency, and survival rates. Furthermore, the fish-fed high-fat diet demonstrated reduced digestive enzyme activity (lipase and amylase), growth hormone (GH), and antioxidative responses (SOD, CAT, and GPx). The intestine of tilapia-fed HF showed also impaired histomorphological features such as reduced villi length and width. However, supplementation of BA in HF diets improved intestinal and villi architecture. The HF diet without BA supplementation revealed massive hepatic steatosis pancreatic vascular congestion and a high necrotic pancreatic cells number. While supplementing BA in HF diets enhanced pancreatic architecture and decreased necrotic hepatocytes. Fish-fed HF diet also showed increased total cholesterol, triglycerides, glucose, ALP, and AST levels as well as the malondialdehyde level (MDA). On the other hand, fish-fed LF and HF/BA diets showed improved growth performance, lipase and amylase, GH, SOD, CAT, and GPx, and intestinal histo-morphology and reduced glucose, leptin, and MDA levels. Dietary BA at 0.15–0.2 g/kg improved the digestion, and antioxidative capacity. In conclusion, dietary BA is required to mitigate the negative impacts of high-fat diets on Nile tilapia growth performance, general health, and antioxidative response.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102605"},"PeriodicalIF":3.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Probiotics Red Yeast Rhodotorula paludigena CM33 on enhancing color pigmentation, antioxidant activity, immune response, intestinal microbiota, and growth in a commercial ornamental fish: Flowerhorn fish","authors":"Jirawadee Kaewda ,&nbsp;Papungkorn Sangsawad ,&nbsp;Surintorn Boonanuntanasarn ,&nbsp;Pimpisut Manassila ,&nbsp;Apichat Boontawan ,&nbsp;Mariena Ketudat‑Cairns ,&nbsp;Cheeranan Sriphuttha ,&nbsp;Chatsirin Nakharuthai","doi":"10.1016/j.aqrep.2024.102609","DOIUrl":"10.1016/j.aqrep.2024.102609","url":null,"abstract":"<div><div>The application of novel feed additives, including yeast-based probiotics, has emerged as a strategy to enhance growth, health, and aesthetic appeal in ornamental fish. This study evaluated the probiotic properties of red yeast <em>Rhodotorula paludigena</em> CM33 in flowerhorn fish (initial weight 9.81 ± 1.25 g). Forty fish were randomly distributed into individual glass tanks and subjected to four feeding treatments: a non-supplemented control diet and diets supplemented with 0.5 %, 1 %, and 2 % <em>R. paludigena</em> CM33 for 105 days. Results indicated that <em>R. paludigena</em> CM33 exhibits comprehensive characteristics validating it as a potential probiotic, demonstrating resistance to various bile salts, pH levels, and thermotolerance. It presented significant antioxidant activity, auto-aggregation, hydrophobicity, positive catalase activity, non-hemolytic properties, and resistance to 14 tested antibiotics. After the feeding trial, fish fed diets supplemented with 1 % and 2 % <em>R. paludigena</em> CM33 showed significant improvements in growth performance, lysozyme activity, total immunoglobulin levels, and antioxidant enzyme activity. These findings correspond with the mRNA expression results of growth-related genes (insulin-like growth factor-1 (<em>IGF-I</em>) and <em>IGF-II</em>), antioxidant genes (superoxide dismutase (<em>SOD</em>) and catalase (<em>CAT</em>)), and pro-inflammatory genes (interleukin-1 beta (<em>IL-1β</em>) and <em>IL-6</em>). Microbiome analysis indicated that incorporating 1 % <em>R. paludigena</em> CM33 into the diet reduced species richness in the intestinal tract of flowerhorn fish. However, both 1 % and 2 % <em>R. paludigena</em> CM33 increased the abundance of <em>Cetobacterium</em>. Field emission scanning electron microscopy confirmed the colonization of <em>R. paludigena</em> CM33 in the intestines of treated fish; meanwhile, fish skin coloration and carotenoid accumulation demonstrated the color enhancement properties of <em>R. paludigena</em> CM33 in flowerhorn fish. These findings suggest that <em>R. paludigena</em> CM33 could be a promising sustainable aquafeed ingredient for the ornamental aquaculture industry.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102609"},"PeriodicalIF":3.2,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}