Aquaculture Reports最新文献

{"title":"Removal efficiency of nitrogen and phosphorus in mariculture effluent by an immobilized algae-bacteria system","authors":"Junze Wu ,&nbsp;Tong Li ,&nbsp;Junting Li ,&nbsp;Yongzhi Chen ,&nbsp;Shafira Citra Desrika Putri ,&nbsp;Yuge Peng ,&nbsp;Ning Zhang ,&nbsp;Yulei Zhang","doi":"10.1016/j.aqrep.2025.103128","DOIUrl":"10.1016/j.aqrep.2025.103128","url":null,"abstract":"<div><div>To explore the removal efficiency of nitrogen and phosphorus in mariculture effluent, <em>Oocystis borgei</em> and <em>Algoriphagus marincola</em> were used to construct an immobilized algae-bacteria system embedded in calcium alginate. Various initial concentrations of ammonia nitrogen (2.5, 10, 30, and 50 mg/L) and carbon (5, 10, 15, and 25 mg/L) with a constant initial phosphorus concentration of 3 mg/L were set to investigate the efficiency of the algae-bacteria system in removing nitrogen and phosphorus from simulated mariculture effluent. The results showed that within a certain range, as the concentration of ammonia nitrogen increased, the removal efficiency of the algae-bacteria system for both nitrogen and phosphate improved, with ammonia nitrogen removal reaching 17.75–20.08 mg/L and phosphate removal rates exceeding 85 % when the ammonia nitrogen concentration was above 30 mg/L. At a carbon concentration of 5 mg/L, the algae-bacteria system had a greater ability to remove nitrogen and phosphorus, with an ammonia nitrogen removal rate of 80.8 % and a phosphate removal rate of 99 %. Compared to the efficiency of single algae or bacteria, the absorption capacity of nitrogen and phosphorus by the algae-bacteria system was significantly enhanced (<em>P</em> &lt; 0.05). The study showed that the combined use of <em>O. borgei</em> and <em>A. marincola</em> can effectively remove ammonia nitrogen and phosphate in mariculture effluent. This research provides a theoretical basis for the application of immobilized <em>O. borgei</em> and <em>A. marincola</em> in the treatment of industrial mariculture effluent.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103128"},"PeriodicalIF":3.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential of integrated multi-trophic aquaculture as an alternative to traditional shrimp farming: A global review of environmental, economic and social sustainability","authors":"Md. Nezum Uddin ,&nbsp;Md. Akhtaruzzaman Khan ,&nbsp;Max Nielsen ,&nbsp;Rasmus Nielsen","doi":"10.1016/j.aqrep.2025.103122","DOIUrl":"10.1016/j.aqrep.2025.103122","url":null,"abstract":"<div><div>While research shows that integrated multi-trophic aquaculture (IMTA) is environmentally advantageous for shrimp farming, traditional mono- and polyculture systems remain the dominant farming methods. Hence, the documented potential of IMTA in addressing growing concerns over environmental impacts such as nutrient and greenhouse gas emissions, reductions in mangrove forest areas and the spread of diseases, remains underutilized in shrimp farming. This article analyses the potential of IMTA to reduce the environmental impacts of shrimp farming while simultaneously being economically and socially sustainable. Founded on the PRISMA methodology, the environmental, economic, and social sustainability of IMTA in shrimp farming was identified through a systematic review of the global literature. Environmentally, the results show that IMTA systems can effectively remove surplus nutrients, balance the nutrient budget, and reduce carbon emissions from farms. Economically, IMTA increases farmers' profit compared to traditional production systems. The social dimension remains unexplored, although stakeholders find that IMTA for species other than shrimp is more socially acceptable than traditional production systems. The implication is that IMTA seems a promising alternative to traditional production systems for achieving sustainability in all three dimensions simultaneously.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103122"},"PeriodicalIF":3.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microflora, gut morphometry and haemato-biochemical analyses of juvenile Clarias gariepinus fed dietary Lactobacillus plantarum and guava (Psidium guajava)","authors":"Seyramsarah Blossom Setufe ,&nbsp;Emmanuel Kolawole Ajani ,&nbsp;Benjamin Obukowho Emikpe ,&nbsp;Samuel Temitope Ogunbanwo ,&nbsp;Senam Patrick Fatsi ,&nbsp;Berchie Asiedu ,&nbsp;Andrews Apraku ,&nbsp;Sajjad Pourmozaffar ,&nbsp;Ibrahim Adeshina","doi":"10.1016/j.aqrep.2025.103120","DOIUrl":"10.1016/j.aqrep.2025.103120","url":null,"abstract":"<div><div>Introducing probiotics and phytobiotics into fish feed is an effective strategy to reduce production costs and improve profit margins. However, there is limited information available regarding their application in aquaculture nutrition. This research evaluated the effects of diets enriched with <em>Lactobacillus plantarum</em> and <em>Psidium guajava</em> leaf meal on gut microflora, histomorphometry, water quality, and blood indices of juvenile <em>Clarias gariepinus</em> (initial weight: 7.48 ± 0.2 g) over an 84-day feeding trial. At the end of a-84 day feeding trial, fish samples were taken from seven treatment groups: C-Control experiment of basal diet of 42 % crude protein level, 40LPc (40 ×10⁵ ml <em>L. plantarum</em> per kg of feed), 60LPc (60 ×10⁵ ml <em>L. plantarum</em> per kg of feed), 80LPc (80 ×10⁵ ml <em>L. plantarum</em> per kg of feed), 40PGLM (40 g <em>P. guajava</em> per kg basal diet), 60PGLM (60 g <em>P. guajava</em> per kg basal diet) and 80PGLM (80 g <em>P. guajava</em> per kg basal diet) with three replicates. There was significant difference (P &lt; 0.05) in the <em>Enterobacteriaceae</em> count between the control (4.3 ± 0.2) log₁₀cfu/g and other treatment groups. There was significant difference (P &lt; 0.05) in the total viable count between 80PGLM (4.5 ± 0.2) log₁₀cfu/g and other treatment groups which included control, 80LPc (4.7 ± 0.2) log₁₀cfu/g and 60PGLM (4.8 ± 0.2) log₁₀cfu/g respectively. Diets containing 40PGLM showed the highest levels of Hb (hemoglobin) and RBC (red blood cell) (P &lt; 0.05). Fish-fed diets containing 40LPc and 80 PGLM showed a significant increase in total protein (TP) and heterophils (Het) (P &lt; 0.05). Lower AST (aspartate aminotransferase), ALP (alkaline phosphatase), and ALT (alanine aminotransferase) were also exhibited in the <em>L. plantarum</em> and <em>P. guajava</em> groups (P &lt; 0.05). The highest villi height (93.6 ± 0.0 µm), villi width (31.5 ± 0.01 µm) and cryptal depth (30.5 ± 0.0 µm) were observed in 40LPc treatment group (P &lt; 0.05). Increase in the proliferation of <em>Enterobacteriaceae</em> in <em>C. gariepinus</em> juveniles fed dietary <em>L. plantarum</em> enhanced the growth of beneficial bacteria in fish gut<em>.</em> According to these results, a diet containing 40 LPC is suitable for feeding <em>C. gariepinus</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103120"},"PeriodicalIF":3.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naringenin alleviates Proteus mirabilis-triggered biochemical disruptions, histopathological shifts, and oxidative stress in Nile tilapia","authors":"Afaf N. Abdel Rahman ,&nbsp;Gehad E. Elshopakey ,&nbsp;Ahmed S. Alsaqufi ,&nbsp;Abdallah Tageldein Mansour ,&nbsp;Yousef Alkhamis ,&nbsp;Hesham A. Hassanien ,&nbsp;Ahmed Abbas ,&nbsp;Abdelhakeem El-Murr ,&nbsp;Rowida E. Ibrahim ,&nbsp;Mohamed Fouad Mansour","doi":"10.1016/j.aqrep.2025.103126","DOIUrl":"10.1016/j.aqrep.2025.103126","url":null,"abstract":"<div><div>The urge to develop a novel, environmentally friendly antimicrobial is growing, particularly in view of the increasing pathogens' resistance to the current therapies. Thus, this study represents the first initiative to address the antibacterial characteristics of naringenin (NAR; 17.50 mg/L) as a water additive flavanone dissolved in dimethyl sulfoxide (DMSO) to support the resistance of Nile tilapia (<em>Oreochromis niloticus</em>) against <em>Proteus mirabilis</em> (bacterial infection). For 15 days, a total of 250 fish (24.30 ± 1.12 g) were split into five groups [control, DMSO, NAR, <em>P. mirabilis</em>, and NAR+<em>P. mirabilis</em>]. Findings showed that <em>P. mirabilis</em> triggered a renal-hepatic dysfunction, as evidenced by a significant increase in creatinine, alanine aminotransferase, and aspartate aminotransferase levels (<em>P</em> &lt; 0.001), and increased fish mortality (40.00 %). Oxidative damage and stress response were induced by <em>P. mirabilis</em> challenge, where a noteworthy rise in the malondialdehyde, cortisol, and glucose concentrations, and a drop in glutathione peroxidase and catalase activities were noted (<em>P</em> &lt; 0.001). A substantial decrease in the neurotransmitter (serotonin and dopamine) concentrations was observed in the <em>P. mirabilis</em> group (<em>P</em> &lt; 0.001). The histopathological shifts in the gills (distorted most gill filaments), kidney (necrotic changes within renal tubules and atrophic glomerular tuft), liver (fatty degeneration and congested portal vein), and brain (pyknotic neurons and hyperplasia of glia cells) were the outcome of <em>P. mirabilis</em> infection. Intriguingly, NAR water treatment reversed all the negative effects of <em>P. mirabilis</em> infection and decreased fish mortality (14.00 %). In addition, NAR lowered bacterial load in the tissues (gills, kidney, liver, and brain) of the <em>P. mirabilis</em>-infected fish. By emphasizing NAR's ability to enhance the physiological and antioxidant status of Nile tilapia challenged with <em>P. mirabilis</em>, this trial highlights the essential significance of NAR as an exceptional anti-bacterial to sustain the aquaculture industry.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103126"},"PeriodicalIF":3.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness evaluation of five probiotics on shrimp and water environment","authors":"Yanmei Tong ,&nbsp;Minglin Zhang ,&nbsp;Qiangyong Li ,&nbsp;Xiaodong Hu ,&nbsp;Yuheng Wei ,&nbsp;Wen Zhao ,&nbsp;Xiaoli Yu ,&nbsp;Min Peng ,&nbsp;Chunling Yang ,&nbsp;Xiuli Chen ,&nbsp;Tingjun Hu ,&nbsp;Yongzhen Zhao","doi":"10.1016/j.aqrep.2025.103110","DOIUrl":"10.1016/j.aqrep.2025.103110","url":null,"abstract":"<div><div>To solve the aquaculture farmers’ difficulty in choosing suitable probiotics, we evaluated the five-probiotics sold in the market. Here, Bacillus subtilis-based product, compound Bacillus-based product (BC), nitrifying bacteria-based product (NB), photosynthetic bacteria-based product (PB) and aquatic lactic acid bacteria-based product (AL) were sprayed into the aquaculture water for 42 days, and shrimp performance and the water environment were measured. The results showed that NB, PB, and AL significantly increased the shrimp growth performance (<em>p</em> &lt; 0.05). The five probiotics enhanced AMS activity significantly (<em>p</em> &lt; 0.01). BC, PB, and AL reduced MDA, GOT, and GPT activity, while BS increased the <em>Vibrio</em> resistance and SOD, CAT, GSH-PX activity. The 16 s sequencing analysis revealed that compared to the control group, the AL and PB groups had significantly different microbial compositions, fewer unique ASVs, and lower intestinal microbiota abundance. <em>Prevotella_7</em>, <em>Muribaculaceae_unclassified</em>, and <em>Veillonella</em> were negative correlation with growth performance significantly, and <em>Enterobacter</em>, <em>Hafnia-Obesumbacterium, Pseudomonas</em>, and <em>Klebsiella</em> were positive correlation with immunity index (<em>p</em> &lt; 0.05). In the control group, 72 % of the gut microbiota originated from the culture water, and this proportion increased after probiotics were added. All five probiotics improved water quality, especially AL. These data suggested that different probiotics have distinct preferences. AL showed the best overall performance, including high growth, favorable gut microbiota, and improved water quality. BS played a key role in enhancing shrimp resistance to <em>Vibrio</em> and nonspecific immunity. This study helped improve the efficacy assessment of aquatic probiotics and provides a theoretical basis for selecting suitable probiotics.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103110"},"PeriodicalIF":3.7,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unifying waste conversion and endangered species conservation in polyculture production with Lake Constance whitefish and noble crayfish","authors":"Dibo Liu ,&nbsp;Matthias Hundt ,&nbsp;Eric Bollinger ,&nbsp;Sebastian Geissler ,&nbsp;Kai Lehmann ,&nbsp;Norbert Wagemann ,&nbsp;Jochen P. Zubrod ,&nbsp;Ralf Schulz","doi":"10.1016/j.aqrep.2025.103112","DOIUrl":"10.1016/j.aqrep.2025.103112","url":null,"abstract":"<div><div>Bioconversion is a sustainable method to recycle nutrient-rich aquaculture waste. This study explored waste bioconversion in the context of species conservation through a polyculture system involving the endangered Lake Constance whitefish (<em>Coregonus macrophthalmus</em>) and European noble crayfish (<em>Astacus astacus</em>). Whitefish served as the primary species producing solid waste (faeces and uneaten feed), while crayfish acted as an extractive recycler. Preliminary trials showed that crayfish which fed solely on whitefish faeces had a 59 % (95 % CI: 25–78 %) reduction in specific growth rate (SGR) compared to those fed on whitefish feed. However, mixing wheat with faeces in a 1:1 ratio offset the growth reduction, so wheat was supplementarily provided to crayfish in the following polyculture experiment. The present study was conducted in a cumulative treatment design: whitefish monoculture, non-separated and mesh-separated whitefish-crayfish polyculture. Growth and survival of both species were assessed, alongside dietary contributions to crayfish from whitefish solid waste and supplementary wheat using stable isotope analysis. Survival analysis revealed a negative interaction in non-separated polyculture, adversely affecting whitefish survival. Mesh separation eliminated interspecific interaction but restricted whitefish access to uneaten pellets, reducing whitefish SGR by 0.16 (95 % CI: 0.12–0.31). Meanwhile, assimilation of solid waste by crayfish increased from 33 % to 45.6 %, probably due to their consumption of uneaten pellets. In conclusion, mesh separation improved species compatibility in polyculture, with crayfish efficiently converting uneaten pellets into biomass, outperforming their use of whitefish faeces. Introducing a detritivore species with a trophic level lower than crayfish may enhance bioconversion efficiency and system sustainability even further.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103112"},"PeriodicalIF":3.7,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of stocking density and feeding frequency on nervous necrosis virus and iridovirus susceptibility in hybrid grouper (Epinephelus lanceolatus ♂×Epinephelus fuscoguttatus ♀)","authors":"Hsiang-Chieh Chuang ,&nbsp;Tsung-Yu Yang ,&nbsp;Jin-Feng Tsai ,&nbsp;Nai-Yu Chen ,&nbsp;Yu-Shen Lai ,&nbsp;Yin-Hsuan Chen ,&nbsp;Ann-Chang Cheng","doi":"10.1016/j.aqrep.2025.103111","DOIUrl":"10.1016/j.aqrep.2025.103111","url":null,"abstract":"<div><div>Stocking density and feeding frequency are key factors affecting the cannibalism of groupers. Additionally, nervous necrosis virus (NNV) and grouper iridovirus (GIV) are two viruses associated with high mortality in the grouper breeding industry. They are key pathogens causing major economic and industrial losses. Hybrid grouper is currently the main species in grouper culture. Therefore, the purpose of this study was to explore the effects of different stocking densities and feeding frequencies on cannibalism and NNV and GIV infections of hybrid groupers. Different stocking densities (5, 10, 20, 30, 40, and 50 fish/L) and different feeding frequencies (2, 4, 6, and 8 times/day) were used to observe the effects on the survival, cannibalism, post-infection mortality, viral gene expression, antibody titer and pathological examination of hybrid groupers. The results showed that a stocking density of 5 fish/L and a feeding frequency of 2 times /day significantly increased cannibalism, whereas a stocking density of 30–40 fish/L and a feeding frequency of 4–6 times/day significantly decreased cannibalism. The highest mortality rate, viral gene expression, and antibody titer after NNV and GIV infection were observed in the group with 50 fish/L and 8 times/day, respectively, and histopathological changes were also observed. Therefore, a stocking density of 30–40 fish/L and a feeding frequency of 4–6 times/day during the early stage of fry rearing combined with the standardized operation process for fry may reduce cannibalism, increase survival, and reduce disease outbreaks.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103111"},"PeriodicalIF":3.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145119152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of photoperiod on feeding, gut microbiota and growth of razor clam Sinonovacula constricta","authors":"Qijun Li ,&nbsp;Qiyi You ,&nbsp;Yinghui Dong ,&nbsp;Hanhan Yao","doi":"10.1016/j.aqrep.2025.103113","DOIUrl":"10.1016/j.aqrep.2025.103113","url":null,"abstract":"<div><div>In the present study, we aimed to explore the effects of photoperiod on the feeding, gut microbiota and growth of razor clam <em>Sinonovacula constricta</em>, and to ascertain the optimal feeding period based on the most favorable photoperiod. Five photoperiod groups (12D:12 L, 14D:10 L, 16D:8 L, 18D:6 L, 20D:4 L) and four zeitgeber time points (ZT0/08:00, ZT6/14:00, ZT12/20:00, ZT18/02:00) were set, and the oxygen consumption, ammonia excretion, feeding rate, digestive enzyme activities (α-Amylase, Pepsin and Lipase) and related gene (<em>AMS</em>, <em>PEP</em>, <em>LPS</em>) expression patterns, gut microbiota and growth performances were analyzed. The results showed that the feeding rate was higher under short-day photoperiods (16D:8 L, 18D:6 L, 20D:4 L) than long-day photoperiods (12D:12 L, 14D:10 L), but the digestive enzyme activity and related gene expression levels were higher under 16D:8 L and 18D:6 L than 20D:4 L. In the growth experiment, the razor clams exhibited significantly higher shell length growth rate, weight gain rate and specific growth rate under the 18D:6 L photoperiod compared to other photoperiods (<em>P</em> &lt; 0.05). The results of <em>16S rRNA</em> gene amplicon sequencing showed that the gut microbiota diversity and growth-related microbial flora abundance were higher under 18D:6 L. According to the data of different light and dark periods under 18D:6 L, the oxygen consumption and ammonia excretion rates, feeding rate, digestive enzyme activities and gene expression levels were all higher at ZT12–20 (20:00–4:00) compared with other time periods. Collectively, these results suggested that the photoperiod 18D:6 L may facilitate the feeding, digestion, metabolism and promote the growth, and increasing the food supply at 20:00–4:00 during night can further promote growth of razor clams.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103113"},"PeriodicalIF":3.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145119151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of biofluorescence emitted by the Pacific whiteleg shrimp Litopenaeus vannamei during a stocking density trial","authors":"Thomas Juhasz-Dora ,&nbsp;Paolo Gamberoni ,&nbsp;Stein-Kato Lindberg ,&nbsp;Matthew Slater","doi":"10.1016/j.aqrep.2025.103124","DOIUrl":"10.1016/j.aqrep.2025.103124","url":null,"abstract":"<div><div>Welfare monitoring methods for the Pacific whiteleg shrimp <em>Litopenaeus vannamei</em> are currently limited to manual visual assessments. This widely cultured crustacean is increasingly reared under high stocking densities, and the development of non-invasive, automated monitoring tools is essential to improve welfare outcomes and farming performance. In this exploratory study, we recorded and quantified the fluorescent emissions of <em>L. vannamei</em> for the first time, investigating changes in biofluorescence in response to handling and a 3-h increased stocking density trial. <em>Litopenaeus vannamei</em> produces fluorescent emissions characterized by a broad green slope (∼ 500–560 nm) followed by a pronounced red secondary peak (∼ 700–730 nm). However, the intensity and anatomical areas of fluorescence were similar across triplicate experimental groups (n = 30) during pre-sampling, significant shifts were observed post-sampling. High individual variability was observed in the spectral data emitted from sampled haemolymph, with no clear relationship between external and haemolymph fluorescence at group level. This study suggests how to extract features from hyperspectral images that can be used for quantifying fluorescence emissions from <em>L. vannamei</em>, and the results presented here demonstrate the importance of proper handling of the shrimp to acquire useful data. The species is considered ideal for exploring digital optical monitoring of biofluorescence. Future research should establish baseline fluorescence profiles, which could serve as a non-invasive, real-time method to monitor health status and welfare in aquaculture operations.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103124"},"PeriodicalIF":3.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145119153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of sterile transgenic fluorescent marine medaka by genetic manipulation","authors":"Jing Huang ,&nbsp;Yabo Cai ,&nbsp;Zhuo Huang ,&nbsp;Shi Xi Chen","doi":"10.1016/j.aqrep.2025.103114","DOIUrl":"10.1016/j.aqrep.2025.103114","url":null,"abstract":"<div><div>The generation of sterile transgenic ornamental fish is the most effective way to prevent ecological risks. In this study, we initially generated a transgenic fluorescent marine medaka (<em>Oryzias melastigma</em>) expressing mCherry under the control of the zebrafish <em>mylz2</em> promoter using the Tol2 transposase system. Subsequently, we utilized CRISPR/Cas9 technology to edit the <em>vasa</em> gene, also known as DDX4, in these transgenic fish <em>Tg</em>(<em>mylz2:mCherry</em>). By injecting a single guide RNA (gRNA) into one-cell-stage embryos, we induced indel DNA mutations. Moreover, the co-injection of multiple gRNAs targeting various exons resulted in the deletion of large DNA fragments. Additionally, the simultaneous delivery of multiple gRNAs significantly increased the mutation rate. The homozygous <em>vasa</em> mutants, characterized by a single amino acid deletion (valine) in motif IV of the Vasa protein, displayed male phenotypes. These males exhibited normal courtship behavior and were capable of inducing spawning with wild-type females. However, the eggs produced from these matings were unfertilized. Histological and gene expression analyses revealed that the gonads of these mutants contained testis tubules devoid of germ cells. Taken together, our findings suggest that the sexual differentiation of somatic gonadal cells in <em>Oryzias</em> species is dependent on the germ cells, and the valine in motif IV is essential for the functional integrity of the Vasa protein.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103114"},"PeriodicalIF":3.7,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}