Aquaculture Reports最新文献

{"title":"Reproductive achievement of one-year-old Pacific abalone (Haliotis discus hannai) following nine generations of selective breeding for high yield and its seedling performance","authors":"Jiaqi Li ,&nbsp;Suyan Xue ,&nbsp;Ershuai Huang ,&nbsp;Lirong Chang ,&nbsp;Yuze Mao","doi":"10.1016/j.aqrep.2025.102766","DOIUrl":"10.1016/j.aqrep.2025.102766","url":null,"abstract":"<div><div>The development of high-yielding aquatic strains represents a critical pathway for optimizing resource allocation and reducing production costs in sustainable aquaculture systems. Selective breeding serves as an effective strategy to further enhance the yield potential of cultivated organisms. In this study, we report the performance of an abalone strain (HX) that had undergone intensive mass selection for high-yield at one year of age for nine generations. Results showed that seven-month-old juvenile abalone from the HX strain (with a shell length of 34.41 ± 3.70 mm and a wet weight of 5.54 ± 1.56 g) were significantly larger (<em>P</em> &lt; 0.05) than those from the control strain (with a shell length of 27.43 ± 2.56 mm and a wet weight of 2.93 ± 0.79 g). Compared to parental abalones from the control group, the larger parental abalones from the HX strain produced more oocytes with larger yolk, resulting in offspring with a significantly higher (<em>P</em> &lt; 0.05) metamorphosis rate. In terms of artificial feed utilization, juvenile abalone from the HX strain spent significantly more time (<em>P</em> &lt; 0.05) feeding than those from the control strain. Furthermore, the organic matter and protein contents in the mixture of residual feed and feces in the HX strain’s cultivating ponds were 25.9 % and 29.3 % lower than those in the control strain, respectively. These findings indicate that abalones from the HX strain not only exhibit improved feed consumption capacity but also possess better nutrient utilization ability.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102766"},"PeriodicalIF":3.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143703912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the potential of nutmeg oil: A sustainable alternative for rainbow trout anesthesia in aquaculture","authors":"Mert Minaz ,&nbsp;Akif Er ,&nbsp;Kübra Ak ,&nbsp;Osman Serdar","doi":"10.1016/j.aqrep.2025.102773","DOIUrl":"10.1016/j.aqrep.2025.102773","url":null,"abstract":"<div><div>In aquaculture, interest in natural anesthetic agents as alternatives to synthetic anesthetics has been steadily increasing. This is due to concerns over residue accumulation, environmental impact, and growing demand for sustainable practices. Within this context, the present study focused on the anesthetic effects of nutmeg essential oil at three different concentrations in rainbow trout (<em>Oncorhynchus mykiss</em>, 94.42 ± 23.9 g): low (400 µL/L), medium (600 µL/L), and high (800 µL/L). Induction and recovery times were recorded, and samples were collected at 0, 2, 4, and 8 hours post-anesthesia for antioxidant, cortisol, and hematological analyses. Additionally, gill tissues were sampled immediately after anesthesia for histological examination. While the induction time for the low concentration exceeded 3 minutes, the induction and recovery times for the other concentrations ranged within acceptable limits (3 min for induction and 5 min for recovery). Blood parameters exhibited an increasing trend until the 4th hour post-anesthesia, peaking at the 2nd hour, before returning to control levels by the 4th hour. Histological evaluation revealed healthy gill tissues in the control and low-concentration group (400 µL/L), whereas hyperplasia was observed in the medium (600 µL/L) and high-concentration (800 µL/L) groups. Antioxidant enzyme activity (superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and glutathione peroxidase (GSH-Px)) indicated potential oxidative stress at the high anesthetic concentration. Similarly, cortisol levels were higher at the high concentration, with plasma cortisol showing a slight initial increase followed by a return to normal levels over time. In conclusion, this study highlights that nutmeg oil at a concentration of 600 µL/L may serve as a promising natural alternative to synthetic anesthetics in aquaculture. However, future studies should include a synthetic anesthetic as a control to allow for direct comparisons and a more comprehensive evaluation of its efficacy and physiological effects.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102773"},"PeriodicalIF":3.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143703911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesquite seed flour as a nutrient-rich alternative to fishmeal for common carp fingerlings (Cyprinus carpio): Environmental, growth performance, transcriptomic and intestinal microbiota responses","authors":"María Fernanda Barragán-Longoria ,&nbsp;Karla Jareth Pérez-Viveros ,&nbsp;Arturo Cadena-Ramírez ,&nbsp;Javier Castro-Rosas ,&nbsp;José Roberto Villagómez-Ibarra ,&nbsp;Jesus Hernandez-Perez ,&nbsp;Silvia Hinojosa-Alvarez ,&nbsp;Carlos Alberto Gómez-Aldapa ,&nbsp;Rocio Alejandra Chavez-Santoscoy","doi":"10.1016/j.aqrep.2025.102768","DOIUrl":"10.1016/j.aqrep.2025.102768","url":null,"abstract":"<div><div>This study evaluated the potential of mesquite seed flour (<em>Prosopis laevigata</em>) as an alternative to fishmeal (FM) in the diets of common carp fingerlings (<em>Cyprinus carpio</em>). A mesquite diet (MD) was formulated containing 48.8 % mesquite flour, 24.4 % fishmeal and 20.7 % corn flour. Over a 45-day trial, 1000 fingerlings were distributed across four tanks (two tank replicates per diet). The carried-out methodology included growth parameters and water quality evaluation, RNA sequencing from liver tissue, and 16S sequencing of intestinal microbiota composition. Both MD and FM diets provided optimal water quality conditions for the growth and well-being of fish during the experiment. Growth performance resulted similarly between MD and FM, with no significant differences in weight gain (379.40 ± 232.55 % for FM and 335.08 ± 219.69 % for MD). Transcriptomic analysis revealed differentially expressed genes involved in lipid metabolism, particularly fatty acid degradation and PPAR signaling pathways. Intestinal microbiota evaluation indicated diet-specific modulation in microbial composition since differences in the relative abundance of shared species between diets was identified. Additionally, MD-fed carp showed unique bacterial species, such as <em>Shewanella putrefaciens</em> and <em>Myxococcus xanthus</em>, while FM-fed fish showed <em>Shewanella hanedai</em> and <em>Archangium disciforme</em>. This study represents the first multi-omics approach, incorporating transcriptomics and metagenomics, to evaluate the effects of mesquite meal supplementation in an aquaculture species as an alternative to fishmeal for enhancing aquafeed sustainability.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102768"},"PeriodicalIF":3.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143706099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First record of non-native polychaetes Polydora websteri and P. caeca invading cultured and wild populations of mussels and oysters in the Mediterranean","authors":"Barbara Mikac ,&nbsp;Vasily I. Radashevsky ,&nbsp;Eugenio Fossi ,&nbsp;Victoria V. Pankova ,&nbsp;Marina Antonia Colangelo ,&nbsp;Giuseppe Prioli ,&nbsp;Marco Abbiati ,&nbsp;Federica Costantini","doi":"10.1016/j.aqrep.2025.102713","DOIUrl":"10.1016/j.aqrep.2025.102713","url":null,"abstract":"<div><div>Species of <em>Polydora</em> and related genera are well known molluscs’ pests transported by aquaculture activities and invading new locations worldwide. Their correct identification often requires confirmation by molecular data due to the presence of cryptic species. In the present study, using morphological and molecular data, we identified three non-native <em>Polydora</em> species associated with cultured and wild mussel <em>Mytilus galloprovincialis</em>, Pacific oyster <em>Magallana gigas</em> and European oyster <em>Ostrea edulis</em> in the Adriatic Sea (Italy). The tube-dwelling <em>P. cornuta</em> has been recorded from the Mediterranean since 1990 and is currently classified as one of the worst invaders in soft bottom communities in the region. The shell-boring <em>P. caeca</em> and <em>P. websteri</em> are here reported for the first time in the Mediterranean. This occurrence is a cause for concern, as the regular transfer of molluscs between aquaculture facilities within the Mediterranean basin and with European farms outside the basin may contribute to the further spread of the non-native <em>Polydora</em> in this region. The origin and distribution of these species in the Mediterranean should be further studied.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102713"},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of feeding pattern on growth, immunity and intestinal flora of GIFT tilapia (Oreochromis niloticus) and largemouth bass (Micropterus salmoides)","authors":"Yuning Wang ,&nbsp;Rui Wang ,&nbsp;Ruiyi Yang ,&nbsp;Shaoyu Zhu ,&nbsp;Feng Yao ,&nbsp;Xiaohua Zhang ,&nbsp;Yanou Yang","doi":"10.1016/j.aqrep.2025.102764","DOIUrl":"10.1016/j.aqrep.2025.102764","url":null,"abstract":"<div><div>The omnivorous GIFT tilapia (Genetically Improved Farmed Tilapia (<em>Oreochromis niloticus</em>)) and carnivorous largemouth bass (<em>Micropterus salmoides</em>) were fed in four feeding patterns to compare the compensatory growth for fish species with different feeding habits. These patterns included satiation (control, fed twice a day at satiation), fasting 2 d and re-feeding 8 d and cycling (the daily feeding pattern is the same as that of the control group, the same below), fasting 4 d and re-feeding 16 d and cycling (both cyclical fasting), feeding once every 10 d then 10 d on satiation and cycling (cyclical food restriction), and each pattern is 60 d. The results showed that both fish species failed to obtain compensatory growth during the cyclical fasting. However, both species obtained completely compensatory growth by significantly improving actual feeding rate and feed efficiency ratio during the cyclical food restriction. During the feeding pattern, GIFT tilapia had significantly higher amylase activity, liver and muscle glycogen contents, and significantly lower blood glucose and serum insulin contents. Largemouth bass had significantly higher protease and lipase activities, and lower blood glucose content. Both species had significantly lower relative expression levels of pro-inflammatory factors in livers; GIFT tilapia had significantly lower and largemouth bass had significantly higher anti-inflammatory factors. Furthermore, GIFT tilapia had significantly higher relative abundance of harmful bacteria and lower relative abundance of beneficial bacteria, and intestinal immunity was impaired; largemouth bass changed the opposite, and intestinal immunity was improved. The results suggest that GIFT tilapia improved their carbohydrate utilization efficiency and largemouth bass improved their protein utilization efficiency, in cyclical food restriction, and the two species achieved completely compensatory growth, but GIFT tilapia impaired immunity, and largemouth bass improved immunity.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102764"},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LRP6 regulates growth in Litopenaeus vannamei via the Wnt/β-catenin signaling pathway","authors":"Sheng Wang ,&nbsp;Xiaodi Wang ,&nbsp;Hao Yang ,&nbsp;Xuanzheng Di ,&nbsp;Haoyang Li ,&nbsp;Bin Yin ,&nbsp;Ziwei Liu ,&nbsp;Chaozheng Li ,&nbsp;Jianguo He","doi":"10.1016/j.aqrep.2025.102769","DOIUrl":"10.1016/j.aqrep.2025.102769","url":null,"abstract":"<div><div>Low-density lipoprotein receptor-related protein 6 (LRP6) is a crucial coreceptor in the canonical Wnt/β-catenin signaling pathway, that plays a fundamental role in cell growth, development, proliferation, and apoptosis. However, little is known about its effect on growth performance in economic animal including shrimp. In this study, we characterized a homolog of LRP6 in shrimp <em>Litopenaeus vannamei</em>, named LvLRP6, and explored its involvement in growth regulation. Multiple sequence alignments and phylogenetic analyses indicated that LvLRP6 belongs to the invertebrate LRP6 family and has conserved phosphorylation sites with those of LRP6 in other species. Functional investigations via RNAi technology revealed that silencing LvLRP6 via dsRNA injection resulted in a significant reduction in shrimp growth, as indicated by lower weight gain than that in the control group. Mechanistic exploration revealed that LvLRP6 positively modulates the Wnt/β-catenin pathway, leading to increased expression of core components such as glycogen synthase kinase 3 (LvGSK3), β-catenin (Lvβ-catenin), and T-cell factor (LvTcf). Furthermore, LvLRP6 exerted a suppressive effect on LvMSTN (myostatin), a negative regulator of muscle-related genes, while concomitantly increasing myosin levels. Subsequent research revealed that the promoter of LvMSTN contains TCF binding motifs. Dual-luciferase reporter experiments confirmed that the overexpression of LvLRP6 or LvTcf reduced the promoter activity of LvMSTN. Additionally, LvTcf was found to induce the expression of LvLRP6 and a number of Wnt ligands, thus amplifying Wnt/β-catenin pathway activation and reinforcing the growth-promoting feedback loop. The present investigation demonstrated the importance of LRP6 in shrimp growth, which contributes to our knowledge of the molecular processes that underlie crustacean growth and offers a theoretical basis for breeding and genetic enhancement initiatives aimed at enhancing shrimp aquaculture growth performance.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102769"},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of transcriptome and metabolome reveals tolerance mechanism to high salinity stress in Crassostrea hongkongensis","authors":"Yixin Zhang ,&nbsp;Qiwen Liang ,&nbsp;Feifei Yu ,&nbsp;Jiexiong Zhu ,&nbsp;Jianfang Gui ,&nbsp;Jinji Lin ,&nbsp;Yishan Lu ,&nbsp;Haiyuan Tao","doi":"10.1016/j.aqrep.2025.102759","DOIUrl":"10.1016/j.aqrep.2025.102759","url":null,"abstract":"<div><div><em>Crassostrea hongkongensis</em> (<em>C. hongkongensis</em>) is an important economic species thriving in intertidal and estuarine areas, and suffers frequent salinity fluctuation. In this study, the transcriptomic and metabolomic were conjointly analyzed to reveal the tolerance mechanism of <em>C. hongkongensis</em> in response to high salinity stress. After high salinity stress at sub-lethal concentration (40 ‰) for 48 h, the enzyme activities of SOD, CAT, LZM, GSH-PX and AKP were measured to confirm that <em>C. hongkongensis</em> had activated the physiological stress reoponses. A total of 232 differentially expressed genes (DEGs) and 968 DEGs were identified after salinity stress for 6 h and 48 h, respectively. DEGs enriched pathways mainly included NOD-like receptor signaling pathway, oxytocin signaling pathway, pentose and glucuronate interconversions, NF-kappa B signaling pathway, apoptosis and so on. A total of 33 differential metabolites (DMs) and 61 DMs were identified in 6 h and 48 h salinity-stress groups. KEGG function analysis showed that DMs mainly involved in free amino acids metabolism, pentose and glucuronate interconversions, fatty acids biosynthesis, aminoacyl-tRNA biosynthesis, pantothenate and CoA biosynthesis and so on. Correlation analysis between DEGs and DMs found that the integrated regulatory network was involved in amino acid metabolism, aminoacyl-tRNA biosynthesis, fatty acid degradation, pyrimidine metabolism, etc. The data suggested that high salinity could cause stress response of <em>C. hongkongensis</em> by adjusting osmotic balance, carbohydrates and lipids metabolism and transfer systems. The study provided important information for understanding molecular mechanism of salinity tolerance in oysters.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102759"},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High inclusion of corn gluten meal supplemented with butyric acid; Impacts on growth, immunology, histopathology, and metabolomics of pacific shrimp","authors":"Mpwaga Alatwinusa Yohana ,&nbsp;Gyan Watson Ray ,&nbsp;Kou Shiyu ,&nbsp;Beiping Tan ,&nbsp;Qihui Yang","doi":"10.1016/j.aqrep.2025.102751","DOIUrl":"10.1016/j.aqrep.2025.102751","url":null,"abstract":"<div><div>This research aimed to examine the impacts of adding butyric acid (BA) at elevated levels to corn gluten meal (CGM) in the Pacific shrimp diet. This study analyzed how adding BA to the diet impacts shrimp growth performance, immunology, metabolomics, and histopathological responses. A total of 720 shrimp were divided into six groups, each receiving a different level of BA supplementation: 0 %, 0.5 %, 1 %, 1.5 %, 2 %, and 2.5 %. The experiment spanned 56 days, with three replicates for each group. The initial mean weight of the shrimp was 0.18 ± 0.00 g. The results of the study indicated that final weight (FW), weight gain rate (WGR), and specific growth rate (SGR) all showed significant increases with increased levels of dietary BA (<em>P</em> &lt; 0.05). The survival rate (SR) was 100 % across all treatment groups. Additionally, dietary BA led to a significant decrease in feed conversion ratio (FCR), with the most pronounced effect observed in the 1.5 % BA group (<em>P</em> &lt; 0.05). The addition of BA to the diet resulted in a significant increase in the activities of alkaline phosphatase (AKP) and acid phosphatase (ACP). At the same time, the levels of aspartate and alanine aminotransferase decreased significantly (<em>P</em> &lt; 0.05). Interestingly, malondialdehyde (MDA) levels decreased with higher BA supplementation, but superoxide dismutase (SOD) activity increased in shrimp-fed BA diets. Histological analysis of the hepatopancreas showed that shrimp-fed diets containing 1 % and 1.5 % BA had significantly higher B and R-cell numbers than the control group. Metabolomics analysis revealed that fatty acids such as palmitic acid, docosahexaenoic acid (DHA), stearic acid, and arachidonic acid were up-regulated in BA diets. The slope broken-line regression analysis of WGR against dietary BA levels suggested that the optimal BA requirement for <em>L. vannamei</em> shrimp was estimated to be 1.25 %.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102751"},"PeriodicalIF":3.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feeding value of low opportunity cost biomasses (agri-food by-products) for development of circular pond fish feeds: An evaluation with common carp (Cyprinus carpio)","authors":"Felix Kofi Agbeko Kuebutornye ,&nbsp;Anil Axel Tellbüscher,&nbsp;Petr Dvořák,&nbsp;Koushik Roy ,&nbsp;Jan Mraz","doi":"10.1016/j.aqrep.2025.102753","DOIUrl":"10.1016/j.aqrep.2025.102753","url":null,"abstract":"<div><div>In many cases, prior to their inclusion in feed, food system by-products need some sort of refinement. Many options are being explored on how these by-products could aid in direct re-generation of food with the shortest value chain. In this study, we hypothesized that common carp raised semi-intensively in European fishponds could convert waste resources to food directly. The 30 % ingredient replacement method was used to evaluate the digestibility of 12 local waste resources of plant origin as candidates for ‘circular feed ingredients’ for feeding pond fish. It was evident from the results that protein digestion (&gt;70 %) is not an issue, even showing similarities between pre-treated resources (brewery wastes) and non-pre-treated resources (oilseed expellers or legumes) – but lysine or methionine decide from 20 % – 49 % utilization of digested protein from those ingredients. It is therefore, recommended that complementary combination of ingredients be explored to overcome limiting DIAAS in natural food base in ponds. In this case, rapeseed expeller and barley distillers’ grain seem to be closest candidates to fix the lysine and methionine limitation in natural pond food base. Microbial/enzymatic/mechanical pre-processed ingredients such as extract of sugar beet, brewery wastes, bakery or extruded breakfast cereals have low pollution potential (P bioavailability 50–80 %) – contrary to proteinaceous resources (oilseed expellers or broken legumes) which had P bioavailability ≤ 25 % (except pea). Bakery or breakfast cereals discards are novel sources of digestible energy for pond fish (∼400 kcal 100 g−1). Given that NDF:ADF ratio (&gt;2:1) allow for easy fermentation in gut, besides possibly laced with microbial enzymes or some pre-hydrolysis, the undigested fibers in sugar beet, legumes, or oilseed expeller (AD carbohydrate 1–20 %) could boost pond food web through microbial upcycling. This study highlights the possibility of developing circular, less-refined semi-intensive pond fish feeds “commercially”. Such feed could enhance resource use efficiency at food-system level.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102753"},"PeriodicalIF":3.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory patterns of testis and ovary maturation in largemouth bass (Micropterus salmoides) revealed by transcriptome profiling","authors":"Qingchun Wang ,&nbsp;Siqi Lu ,&nbsp;Jixiang Hua ,&nbsp;Yifan Tao ,&nbsp;Yan Zhuge ,&nbsp;Wenhua Chen ,&nbsp;Xuejun Duan ,&nbsp;Jun Qiang","doi":"10.1016/j.aqrep.2025.102761","DOIUrl":"10.1016/j.aqrep.2025.102761","url":null,"abstract":"<div><div>Largemouth bass (<em>Micropterus salmoides</em>) is an economically significant farmed fish. It would be useful to understand its gametic maturation to establish effective breeding populations. We conducted histological analyses, transcriptome sequencing, and fluorescent <em>in situ</em> hybridization (FISH) analyses to detect differences in gene expression between ovaries and testes of sexually mature largemouth bass (6.5 months old). Analyses of differentially expressed genes and a gene set enrichment analysis identified 32 genes associated with gametic maturation (17 in ovaries; 15 in testes). Those in the ovaries included <em>elovl1a</em>, <em>hsd17b12a</em>, <em>cidec</em>, and <em>acls5</em>, encoding enzymes involved in lipid biosynthesis in stage III and IV oocytes; <em>lrp13</em> and <em>iars2</em>, encoding proteins involved in yolk particle synthesis; and <em>cpeb1b</em>, <em>cth1</em>, and <em>ccnb1</em>, encoding proteins involved in the initiation and termination of meiosis to ensure oocyte maturation. The important genes in the testes were associated with the formation and assembly of microtubule structures. They included <em>cfap161</em>, <em>spag6</em>, <em>iqub</em>, <em>enkur</em>, <em>tekt1</em>, <em>mns1</em>, <em>gas8</em>, and <em>ccdc40</em>, encoding proteins involved in the regulation of the structural formation of sperm flagella; and <em>lrguk</em>, <em>spef2</em>, and <em>tdrd5</em>, encoding proteins involved in the initiation of spermatid assembly and maturation to form sperm — a critical stage in spermatogenesis. The transcriptome data and FISH results revealed high expression of <em>dmrt1</em> in the extracellular matrix of spermatogonia cells, and of <em>btg4</em> in the cytoplasm of stage IV oocytes. These two genes may serve as crucial regulatory factors in the maturation of testes and ovaries, respectively. Our findings offer insights into the mechanisms underlying gonad maturation in largemouth bass.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102761"},"PeriodicalIF":3.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}