培养物盐度和饲料蛋白质水平对草鱼幼鱼生长、营养物质代谢和免疫反应影响的研究

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-09-02 DOI:10.1016/j.aqrep.2025.103082

Dongyu Huang , Mingchun Ren , Shengqi Zhao , Haifeng Mi , Tao Teng , Junjie Qin , Hualiang Liang , Lu Zhang

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引用次数: 0

摘要

本试验旨在研究培养物盐度和饲料蛋白质水平（DPLs）对草鱼幼鱼生长、营养物质代谢和免疫应答的影响。采用3 × 2因子设计，配制3种不同DPLs（31.19 %、36.03 %和40.18 %）和2种盐度水平（0 ‰和7 ‰）的饲粮。360尾草鱼幼鱼（初始体重3.72 g）分布在18个（180 L）水族箱中。结果表明，在0 ‰和7 ‰盐度条件下，分别添加36.03 %和40.18 %的DPLs可显著提高水稻的生长性能。在营养代谢方面，在0 ‰盐度下，36.03 % DPL上调igf-1、s6k和fas mRNA表达水平，40.18 % DPL上调gk mRNA表达水平；盐度为7 ‰时，与31.19 % DPL组相比，36.03 %和40.18 % DPL组显著上调了s6k1的mRNA表达水平。此外，与0 ‰盐度相比，7 ‰盐度下gk、g6pase、fas和cpt1 mRNA表达水平显著上调。在抗氧化能力方面，盐度为0 ‰时，36.03 % DPL的CAT和SOD活性最高，MDA含量最低；盐度为7 ‰时，DPLs的CAT活性最高，分别为36.03 %和40.18 %。此外，与0 ‰盐度相比，7 ‰盐度显著提高了CAT活性和GSH含量，显著降低了MDA含量。在免疫应答方面，盐度为0 ‰时，36.03 % DPL组的stat3 mRNA表达水平显著降低，盐度为0 ‰时36.03 %和40.18 % DPL组的nfκb和stat3 mRNA表达水平显著降低。与0 ‰盐度相比，7 ‰盐度显著下调stat3mrna表达水平。适当的蛋白质水平（36.03 %和40.18 %）和7 ‰盐度也能显著提高免疫应答。

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A study on the effects of culture salinity and dietary protein levels on growth, nutrient metabolism, and immune response of juvenile grass carp

An 8-week experiment was designed to study the effects of culture salinity and dietary protein levels (DPLs) on the growth, nutrient metabolism, and immune response of juvenile grass carp (Ctenopharyngodon idella). Three diets were formulated with different DPLs (31.19 %, 36.03 %, and 40.18 %) and two salinity levels (0 ‰ and 7 ‰) in a 3 × 2 factorial design. 360 grass carp juveniles (initial weight 3.72 g) were distributed into 18 tanks (180 L). The results presented that the growth performance was increased significantly by 36.03 % DPLs at 0 ‰ salinity, and 40.18 % DPLs at 7 ‰ salinity, respectively. Regarding nutrient metabolism, at 0 ‰ salinity, 36.03 % DPL up-regulated the mRNA expression levels of igf-1, s6k, and fas, and 40.18 % DPL up-regulated the mRNA expression levels of gk; at 7 ‰ salinity, compared to the 31.19 % DPL group, 36.03 % and 40.18 % DPLs significantly up-regulated the mRNA expression levels of s6k1. Furthermore, compared to 0 ‰ salinity, the gk, g6pase, fas, and cpt1 mRNA expression levels were significantly up-regulated at 7 ‰ salinity. Regarding antioxidant capacity, at 0 ‰ salinity, 36.03 % DPL obtained the maximum CAT and SOD activities, and the minimum MDA content; at 7 ‰ salinity, 36.03 % and 40.18 % DPLs obtained the maximum CAT activities. Furthermore, compared to 0 ‰ salinity, the CAT activities and GSH contents were increased, and the MDA contents were decreased significantly at 7 ‰ salinity. Regarding immune response, at 0 ‰ salinity, the stat3 mRNA expression level was significantly decreased in the 36.03 % DPL group, and the nfκb and stat3 mRNA expression levels were significantly decreased in the 36.03 % and 40.18 % DPLs at 0 ‰ salinity. Compared to 0 ‰ salinity, the stat3 mRNA expression levels were significantly down-regulated at 7 ‰ salinity. Furthermore, appropriate protein levels (36.03 % and 40.18 %) and 7 ‰ salinity also significantly improve the immune response.

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.