Dietary lauric acid improves the protein utilization and muscle growth by promoting intestinal flora and glycolysis in mud crab (Scylla paramamosain)

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-04-14 DOI:10.1016/j.aqrep.2025.102816

Wenhao Zhan , Shichao Xie , Yao Deng , Hongyu Peng , Haiqing Cao , Zheng Tang , Yinqiu Tian , Yu Zhang , Tingting Zhu , Min Jin , Qicun Zhou

{"title":"Dietary lauric acid improves the protein utilization and muscle growth by promoting intestinal flora and glycolysis in mud crab (Scylla paramamosain)","authors":"Wenhao Zhan , Shichao Xie , Yao Deng , Hongyu Peng , Haiqing Cao , Zheng Tang , Yinqiu Tian , Yu Zhang , Tingting Zhu , Min Jin , Qicun Zhou","doi":"10.1016/j.aqrep.2025.102816","DOIUrl":null,"url":null,"abstract":"<div><div>At present, the rapid development of aquaculture is restricted by the shortage of fishmeal, and it is particularly important to find ways to replace and reduce fishmeal. It is worth exploring whether feed with lower-than-normal protein requirements have adverse effects on the growth of aquatic animals. The aim of the present study was to improve the growth performance of mud crab (<em>Scylla paramamosain</em>) by adding lauric acid (LA) and glycerol mono-laurate (GML) to a low protein diet (41.8 % crude protein). Adding 1 g/kg LA or 0.5 g/kg GML to low protein diet can significantly ameliorate the negative effects of low protein diet on mud crabs, and improve the survival and growth performance. In addition, dietary LA or GML supplementation can enhance protein apparent digestibility (ADP), increase protein and amino acid content in muscle, and optimize protein metabolism process. Notably, dietary LA and GML supplementation can activate the glycolytic pathway in muscles and up-regulate the expression of genes related to muscle growth. Moreover, the results of intestinal flora indicated that dietary GML and LA increased the abundance of Rhodobacteraceae, Erysipelotrichaceae, Endozoicomonadaceae and <em>Ruegeria</em>. These flora exhibited a positive correlation with protein digestion, absorption and deposition. In conclusion, the supplementation of LA or GML in low-protein diets can effectively improve growth performance, activate muscle glycolysis pathway, promote muscle development, increase microbial abundance related to protein metabolism, and improve protein apparent digestibility.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102816"},"PeriodicalIF":3.2000,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture Reports","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352513425002029","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 0

Abstract

At present, the rapid development of aquaculture is restricted by the shortage of fishmeal, and it is particularly important to find ways to replace and reduce fishmeal. It is worth exploring whether feed with lower-than-normal protein requirements have adverse effects on the growth of aquatic animals. The aim of the present study was to improve the growth performance of mud crab (Scylla paramamosain) by adding lauric acid (LA) and glycerol mono-laurate (GML) to a low protein diet (41.8 % crude protein). Adding 1 g/kg LA or 0.5 g/kg GML to low protein diet can significantly ameliorate the negative effects of low protein diet on mud crabs, and improve the survival and growth performance. In addition, dietary LA or GML supplementation can enhance protein apparent digestibility (ADP), increase protein and amino acid content in muscle, and optimize protein metabolism process. Notably, dietary LA and GML supplementation can activate the glycolytic pathway in muscles and up-regulate the expression of genes related to muscle growth. Moreover, the results of intestinal flora indicated that dietary GML and LA increased the abundance of Rhodobacteraceae, Erysipelotrichaceae, Endozoicomonadaceae and Ruegeria. These flora exhibited a positive correlation with protein digestion, absorption and deposition. In conclusion, the supplementation of LA or GML in low-protein diets can effectively improve growth performance, activate muscle glycolysis pathway, promote muscle development, increase microbial abundance related to protein metabolism, and improve protein apparent digestibility.

查看原文本刊更多论文

饲粮月桂酸通过促进泥蟹肠道菌群和糖酵解提高蛋白质利用和肌肉生长

目前，水产养殖的快速发展受到鱼粉短缺的制约，寻找替代和减少鱼粉的方法显得尤为重要。低于正常蛋白质需取量的饲料是否会对水生动物的生长产生不利影响，值得探讨。本试验旨在通过在低蛋白质（41.8% %粗蛋白质）饲粮中添加月桂酸（LA）和单月桂酸甘油（GML），提高泥蟹（Scylla paramamosain）的生长性能。在低蛋白质饲粮中添加1 g/kg LA或0.5 g/kg GML可显著改善低蛋白质饲粮对泥蟹的负面影响，提高泥蟹的生存和生长性能。此外，饲粮中添加LA或GML可提高蛋白质表观消化率（ADP），增加肌肉中蛋白质和氨基酸含量，优化蛋白质代谢过程。饲粮中添加LA和GML可以激活肌肉中的糖酵解途径，上调肌肉生长相关基因的表达。此外，肠道菌群结果表明，饲粮中添加GML和LA增加了红杆菌科、丹毒科、内生单胞菌科和鲁氏菌属的丰度。这些菌群与蛋白质的消化、吸收和沉积呈正相关。综上所述，在低蛋白质饲粮中添加LA或GML可有效改善生长性能，激活肌肉糖酵解途径，促进肌肉发育，增加与蛋白质代谢相关的微生物丰度，提高蛋白质表观消化率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.