Substituting photoautotrophic with heterotrophic Chlorella species for water quality regulation and microbial community modification in Litopenaeus vannamei pond culture

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-08-19 DOI:10.1016/j.aqrep.2025.103049

Chu-er Song , Zhen Meng , Bangyin Zhang , Lin Yang , Xiaoxuan Sun , Zheng Zhang , Xiaojin Song

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引用次数: 0

Abstract

The study aimed to assess the replacement of photoautotrophic cultivated Chlorella vulgaris with heterotrophic cultivated C. pyrenoidosa as the inoculated algal fluid for analyzing effluent nutrient removal and regulating bacterial community dynamics in Litopenaeus vannamei outdoor pond culture system. Two separate treatment outdoor earthen ponds were utilized; one supplemented with C. vulgaris (CV pond) while the other supplemented with C. pyrenoidosa (CP pond), each stocked at a density of 80 animals m⁻³ in the two ponds for a period of 30 days. Regular sampling was performed on water and L. vannamei individuals from both sets of ponds to evaluate water nutrient parameters, bacterial community composition, and growth parameters correspondingly. The shrimp in the CP pond exhibited a weight gain rate and specific growth rate of approximately 157.32 ± 0.43 % and 3.11 ± 0.01 %, respectively, while those in the CV pond demonstrated rates around 159.53 ± 0.36 % and 3.15 ± 0.01 %, with no statistically significant differences observed between the two groups. Consequently, both ponds displayed similar patterns of microalgae proliferation after inoculation throughout the 30-day experiment, with no significant variation detected in algal cell density. The inoculation with C. pyrenoidosa demonstrated enhanced removal efficiency of PO₄^3--P and NH₄⁺-N, along with regulation of pH and BOD/COD levels in shrimp ponds. Inoculating with C. pyrenoidosa further enhances microbial diversity and richness within aquaculture ponds by promoting an abundance of beneficial bacterial taxa such as Proteobacteria, Actinobacteria, Alphaproteobacteria, Gracilibacteria, Luteolibacter, and Exiguobacterium. Redundancy analysis reveals that microalgae species primarily regulate bacterial communities by exerting influence on water quality factors. These findings offer crucial insights into the utilization of heterotrophic cultivation microalgae for shrimp aquaculture by examining their impact on water quality factors and microbial community structures within shrimp cultural ecosystems.

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异养小球藻替代光自养对凡纳滨对虾池塘水质调节和微生物群落改造的影响

本研究旨在研究异养型培养的pyrenoidosa替代光自养型培养的普通小球藻作为接种藻液，分析凡纳滨对虾室外池塘培养系统出水营养物去除和调节细菌群落动态。采用两个独立处理的室外土池；1个池中分别添加C. vulgaris （CV池）和C. pyrenoidosa （CP池），每个池的放养密度为80只m−3，放养期为30 d。对两组池塘的水体和凡纳美乳杆菌个体进行定期取样，评估水体营养参数、细菌群落组成和生长参数。CP池塘中的虾展出的增重率和特定的增长率大约157.32 ±  % 0.43和3.11 ±0.01  %,分别,而那些在简历池塘了利率约159.53 ±0.36   %和3.15±0.01  %,与观察两组之间没有明显的统计学差异。因此，在30天的试验中，接种后两个池塘的微藻增殖模式相似，藻细胞密度没有明显变化。接种pyrenoidosa可提高对虾池中PO43—P和NH4+-N的去除效率，并可调节pH和BOD/COD水平。通过接种C. pyrenoidosa进一步提高养殖池塘内微生物的多样性和丰富度，促进有益细菌类群如变形菌门、放线菌门、Alphaproteobacteria、Gracilibacteria、Luteolibacter和Exiguobacterium的丰富度。冗余分析表明，微藻主要通过对水质因子的影响来调节细菌群落。这些发现通过研究异养微藻对对虾养殖生态系统中水质因子和微生物群落结构的影响，为利用异养微藻进行对虾养殖提供了重要的见解。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.