Replacement of fish meal with a methanogen bacterial single cell protein: Effects on nutrient utilization and performance in juvenile rainbow trout (Oncorhynchus mykiss)

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-08-13 DOI:10.1016/j.aqrep.2025.103042

Ivar Lund , Pedro Gómez-Requeni , Jørgen Holm , Nana Wentzel Thorringer

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Abstract

Nutrient utilization and performance of juvenile rainbow trout was examined by a gradual dietary replacement of fish meal (FM) with a novel methanotrophic bacterial single cell protein (BSCP) (Uniprotein®) as only dietary protein sources. Six isoproteic and isoenergetic diets included 0 %, 7.2 %, 14.5 %, 21.9 %, 29.3 %, 60.4 % of FM protein replacement with BSCP and without optimization and balancing with crystalline amino acids. Two experiments were conducted: 1) A mass balance study and 2) a six week growth performance - and amino acid (AA) digestibility study. The apparent digestibility coefficient (ADC) of crude protein (CP) significantly decreased (P ≤ 0.04) with ≥ 14.5 % of FM CP replacement. Partial protein digestibility was 86.7 % for BSCP and 93.0 % for FM with tryptophan and cysteine having the lowest AA digestibility. Crude lipid (CL) ADC was lowest at 60.4 % FM CP replacement (P ≤ 0.005). Nitrogen free extract (NFE) ADC was highest at 7.2 % CP replacement (P ≤ 0.047) and lowest at 60.4 % CP replacement (P ≤ 0.001). Total phosphorus (TP) ADC followed dietary TP content and increased by inclusion of BPM (P < 0.02). Solid faeces N excretion (TAN, total ammonia nitrogen) increased with higher FM replacement, conclusive of the lower CP digestibility and utilization of BSCP. Solid P faeces excretion followed dietary content in contrast to dissolved TP, that increased by higher BSCP inclusion. Specific growth rate (SGR) was not affected (P ≥ 0.078), but a negative impact on feed conversion ratio (FCR; P < 0.001) at highest replacement. No adverse gut or histopathological changes were observed. Hepatosomatic index (HSI) increased by supplementation of BSCP. Uniprotein® BSCP can replace at least 7.2 % of FM protein (32 g kg diet⁻¹).

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产甲烷菌单细胞蛋白替代鱼粉对虹鳟鱼幼鱼营养物质利用和生产性能的影响

以新型甲烷营养细菌单细胞蛋白（Uniprotein®）作为饲料蛋白质源，逐步替代鱼粉（FM），研究了虹鳟鱼幼鱼的营养利用和生产性能。6种等蛋白和等能饲料中，用BSCP替代0 %、7.2 %、14.5 %、21.9 %、29.3 %、60.4 %的FM蛋白，且未与晶体氨基酸进行优化和平衡。进行了两个试验：1)质量平衡试验和2)6周生长性能和氨基酸消化率试验。粗蛋白质表观消化系数（ADC）显著降低（P ≤ 0.04），鱼粉粗蛋白质替代率≥ 14.5 %。BSCP和FM的部分蛋白质消化率分别为86.7 %和93.0 %，其中色氨酸和半胱氨酸的AA消化率最低。粗脂肪（CL） ADC最低，为60.4 % FM CP替代（P ≤ 0.005）。无氮提取物（NFE） ADC最高为7.2 % CP替代（P ≤ 0.047），最低为60.4 % CP替代（P ≤ 0.001）。总磷（TP） ADC随饲粮TP含量升高而升高（P <； 0.02）。粪便中固体氮排泄量（TAN、总氨氮）随饲料添加量的增加而增加，说明BSCP对粗蛋白质的消化率和利用率较低。固体磷排泄量随饲料中磷含量的增加而增加，而可溶性磷则随BSCP添加量的增加而增加。对特定生长率（SGR）无影响（P ≥ 0.078），但对饲料系数(FCR；P <； 0.001)。未观察到不良的肠道或组织病理学改变。添加BSCP后肝体指数（HSI）升高。单蛋白®BSCP可替代至少7.2 %的FM蛋白（32 g kg饲粮−1）。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.