The use of synthesized stabilized nanoparticles of selenium as a feed additive in shrimp Litopenaeus vannamei diets: Performance, antibacterial activity, gut microbiota and immune-related genes expression

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-08-16 DOI:10.1016/j.aqrep.2025.103046

Fatma H.A. Mustafa , Seham A.H. Hassan , Ola A. Ashary , Eman M. Abbas , Janice A. Ragaza , Mohamed S. Hassaan , Zaki Z. Sharawy , Marawan Z. Sharawy , Eman H. Zaghloul

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引用次数: 0

Abstract

The shrimp aquaculture industry faces challenges that hinder production, including pathogens and infections from bacteria-causing diseases. The present study aimed to examine the impact of dietary synthesized chitosan stabilized selenium nanoparticles (CSSeNPs) on the growth performance of gut microbiota and immune-associated genes for shrimp L. vannamei aquaculture. The experiments were accomplished for 12 weeks in triplicate fiberglass circular tanks (200 L). A 300 shrimp post-larvae (PL) weighing an average of 0.14 ± 0.001 g was utilized. Four experimental groups were assigned to 12 tanks. Each tank contained 25 PL. Each group fed with one of the following diets: control (C), commercial diet without CSSeNPs (Control; T1), 25 mg/kg CSSeNPs (T2), 35 mg/kg CSSeNPs (T3), and 45 mg/kg CSSeNPs (T4). The specific growth rate (SGR) and feed conversion ratio (FCR) showed improvement (P ≤ 0.05) in the T2 and T3 groups compared to the control group. The relative expression of all examined genes LPS/b-glucan binding protein, glutathione peroxidase and superoxide dismutase (L-GBP, GPX, and SOD) were increased (P < 0.05) at T3. CSSeNPs demonstrated anti-fish pathogen efficacy against Gram-positive and Gram-negative bacteria. The Enterococcus faecalis bacterium displayed the highest vulnerability to inhibition. In contrast, Escherichia coli exhibited the lowest level of susceptibility. Moreover, a quantitative assessment of the prebiotic-like effect of CSSeNPs on shrimp gut showed increased lactic acid bacteria. Also, no Vibrio sp., Salmonella sp., Shigella sp., or Pseudomonas sp. were detected in the T3 group feed on 35 mg/Kg CSSeNPs. This work provides a new safe additive that is eco-friendly for the shrimp diet and improves growth, immunity, and disease control.

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合成稳定纳米硒作为饲料添加剂在凡纳滨对虾饲料中的应用：性能、抗菌活性、肠道微生物群和免疫相关基因表达

对虾养殖业面临着阻碍生产的挑战，包括病原体和细菌引起的疾病感染。本研究旨在研究饲粮中添加合成壳聚糖稳定纳米硒（CSSeNPs）对凡纳滨对虾（lannamei）养殖肠道菌群和免疫相关基因生长性能的影响。实验在3个玻璃纤维圆形槽（200 L）中完成，为期12周。利用300只平均体重0.14 ± 0.001 g的幼虾（PL）。4个实验组，每组12只。每组分别饲喂对照(C)、不添加CSSeNPs的商品饲粮（对照；T1）、25 mg/kg CSSeNPs （T2）、35 mg/kg CSSeNPs （T3）和45 mg/kg CSSeNPs （T4）。与对照组相比，T2和T3组的特定生长率（SGR）和饲料系数（FCR）均有提高（P ≤ 0.05）。在T3时，所有检测基因LPS/b-葡聚糖结合蛋白、谷胱甘肽过氧化物酶和超氧化物歧化酶（L-GBP、GPX和SOD）的相对表达量均升高（P <； 0.05）。CSSeNPs对革兰氏阳性菌和革兰氏阴性菌均有抗鱼致病菌的作用。粪肠球菌最易受抑制。相比之下，大肠杆菌表现出最低的敏感性。此外，定量评估CSSeNPs对虾肠道的益生元样作用显示乳酸菌增加。T3组饲料中35 mg/Kg CSSeNPs未检出弧菌、沙门氏菌、志贺氏菌和假单胞菌。本工作提供了一种新的安全添加剂，对虾的饲料是生态友好的，并促进生长，免疫和疾病控制。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.