Astaxanthin from Phaffia rhodozyma screened by metagenome sequencing improves the growth performance, intestinal health, and immunity of juvenile Opsariichthys bidens

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-07-05 DOI:10.1016/j.aqrep.2025.102960

Muyan Li , Weishao Bu , Zifeng Yin , Muhammad Jawad , Lingzhan Xue , Gaoxiong Zeng , Meiying Wu , Zhang Lu , Junqiang Qiu , Mingyou Li

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引用次数: 0

Abstract

Intestinal flora, known as the "second genome," play an important role in growth, development, barrier protection, and immunological tolerance in aquatic organisms. Intestinal flora of cultured fish can be influenced by various factors such as culture environment, growth stage, growth rate, and gender. In this study, we used a metagenome sequence to analyze the intestinal flora of male and female Chinese hook snout carp (Opsariichthys bidens) across different growth stages. This study aims to identify the genera that serve as sources of dietary nutrient supplementation to improve fish health status and growth performance. The results showed no significant differences in the overall intestinal flora of male and female O. bidens. However, variations in the growth stage may cause changes in the dominant phyla and functional dissimilarities between the two genders. Among the identified genera, Phaffia rhodozyma was screened as a genus considered to have the potential for nutrient supplementation. In order to validate its efficacy, varying concentrations of P. rhodozyma were supplemented into the diet to assess the culture performance of O. bidens. Our results demonstrated that the 0.5 % dietary supplementation of P. rhodozyma significantly improved growth performance indicators (WGR, SCR, and FCR) of O. bidens as compared to the control group (p < 0.05). Moreover, it’s also enhanced the reproductive performance in females as well as increased the antioxidant capacity and immune resistance. Based on growth performance, 0.5 % was determined to be the optimal supplementation level of P. rhodozyma.

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通过宏基因组测序从法菲红酵母中筛选虾青素，可改善bidens Opsariichthys幼鱼的生长性能、肠道健康和免疫力

肠道菌群被称为“第二基因组”，在水生生物的生长发育、屏障保护和免疫耐受中起着重要作用。养殖鱼类肠道菌群受养殖环境、生长阶段、生长速率和性别等因素的影响。本研究利用宏基因组序列分析了不同生长阶段雌雄钩鼻鱼的肠道菌群。本研究旨在确定可作为饲料营养补充来源的属，以改善鱼类的健康状况和生长性能。结果表明，雄、雌梭子蟹肠道菌群总体差异不显著。然而，生长阶段的变化可能导致优势门的变化和两性之间的功能差异。在鉴定的属中，法菲红酵母属被认为具有营养补充潜力。为了验证其有效性，在饲料中添加不同浓度的红酵母，以评估红酵母的培养性能。结果表明，与对照组相比，饲粮中添加0.5 %的红酵母显著提高了黄颡鱼的生长性能指标（增重比、SCR和FCR）（p <； 0.05）。此外，它还提高了雌性的生殖能力，提高了抗氧化能力和免疫能力。根据生长性能，确定0.5 %为红酵母的最佳添加水平。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.