Transboundary and Emerging Diseases最新文献

{"title":"A Self-Amplifying RNA Lipid Nanoparticle (saRNA-LNP) Vaccine Provides Effective Protection Against Porcine Epidemic Diarrhea.","authors":"Jinqi Shu, Han Zhang, Fang Wang, Hanyu Zhang, Xin Liu, Keshu Liu, Yinhe Zha, Wenqin Chai, Jianghui Yao, Yujun Lin, Chao Wu, Xiaomin Zhao","doi":"10.1155/tbed/3115893","DOIUrl":"https://doi.org/10.1155/tbed/3115893","url":null,"abstract":"<p><p>The development of vaccine platforms capable of inducing sustained, potent, and cost-effective immunity remains a major challenge in veterinary vaccinology, particularly for coronaviruses, which are often associated with short-lived vaccine-induced protection. Here, we establish a self-amplifying RNA (saRNA) lipid nanoparticle (LNP) vaccine platform and evaluate its application using porcine epidemic diarrhea virus (PEDV) as a representative coronavirus model. A single vaccination elicited robust specific humoral and cellular immune responses. Vaccinated piglets exhibited significantly higher specific IgG and neutralizing antibody titers of PEDV-S (1:160) at 28 days postvaccination (dpv). Furthermore, elevated levels of serum IFN-γ and IL-4, along with enhanced CD4⁺ and CD8⁺ T cells, were observed. Vaccinated pregnant sows exhibited significantly higher PEDV neutralizing antibody titers (~1:224 or 256) in serum and colostrum samples. The saRNA-LNP vaccine conferred both active immune protection against PED in immunized piglets and passive immunity in neonates via colostrum-derived antibodies from vaccinated sows. Notably, the truncated spike protein antigen outperformed the full-length spike protein in terms of immunogenicity, revealing the importance of careful testing in designing this PEDV saRNA vaccine. The saRNA-LNP system represents a dose-sparing, single-dose vaccine platform with the potential to extend protective immunity, enhance maternal antibody responses, and reduce vaccination costs for PEDV and other veterinary coronaviruses.</p>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2026 ","pages":"3115893"},"PeriodicalIF":3.0,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13147209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological Trends of COVID-19 Infection and Symptom Incidence in China Following the Adjustment of Zero-COVID Policy: A Prospective, Community-Based Cohort Study.","authors":"Zhigao Chen, Zhen Zhang, Yeen Huang, Zhanwei Du, Ruohan Chen, Nixuan Chen, Tingting Liu, Yanpeng Cheng, Honglin Wang, Huawei Xiong, Lixia Song, Yanfen Ye, Yan Lu, Ziquan Lv, Tingting Cao, Yingying Li, Bin Zhu, Xuan Zou, Jianhua Lu, Shenying Fang, Benjamin J Cowling","doi":"10.1155/tbed/5590977","DOIUrl":"https://doi.org/10.1155/tbed/5590977","url":null,"abstract":"<p><p>This study tracked the postadjustment infection rates and symptom trends within community settings. Conducted from May 2023 to May 2024 across four districts in Shenzhen, involving 3246 participants, over 80% had received at least one vaccine dose. Postpolicy adjustment witnessed three significant infection surges. Among the cohort, 63.7% reported only one Coronavirus disease 2019 (COVID-19) infection, 30.3% two infections, and 1.7% three infections, with 4.3% remaining uninfected throughout the follow-up. Older adults and those with lower IgG levels had increased reinfection risk. Notably, 3.8% (95% CI: 3.1-4.6) reported long COVID, with higher susceptibility in those with underlying conditions (adjusted odds ratio [AOR] = 3.73, 95% CI: 2.20-6.34) and reduced incidence among fully vaccinated individuals (AOR = 0.57, 95% CI: 0.36-0.90). The policy change led to widespread exposure, shifting from first infections to reinfections. These insights underscore the need for ongoing research to inform future pandemic responses.</p>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2026 ","pages":"5590977"},"PeriodicalIF":3.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13140162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Novel Epidemiologic Features of High Pathogenicity Avian Influenza Virus A H5N1 2.3.3.4b Panzootic: A Review\".","authors":"","doi":"10.1155/tbed/9758482","DOIUrl":"https://doi.org/10.1155/tbed/9758482","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2024/5322378.].</p>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2026 ","pages":"9758482"},"PeriodicalIF":3.0,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13139616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surveillance of Zoonotic Pathogens in Small Mammals Across Forests With Different Levels of Anthropization in Eastern France.","authors":"Marie Bouilloud, Maxime Galan, Anaïs Bordes, Caroline Tatard, Philippe Gauthier, Julien Pradel, Guillaume Castel, Hussein Alburkat, Lara Dutra, Tarja Sironen, Clémence Galon, Sara Moutailler, Zorée Djelouadji, Benjamin Roche, Nathalie Charbonnel","doi":"10.1155/tbed/4038648","DOIUrl":"https://doi.org/10.1155/tbed/4038648","url":null,"abstract":"<p><p>The emergence of infectious diseases associated with land-use changes is well-documented. However, the presence and dynamics of zoonotic pathogens in small mammals within European forests, whether from rural development or urban greening, remain underexplored. To describe zoonotic hazards in these ecosystems, and to assess the influence of biotic and abiotic factors on their distribution, we analyzed 1549 individuals from 18 small mammal species sampled across forest types representing different levels of anthropization using both targeted and broad-spectrum serological and molecular methods. We detected nine bacteria and five Apicomplexa that are potentially pathogenic to humans. Zoonotic pathogen richness and community composition varied significantly across host species, sites, and sampling periods. Richness was lower in forested urban parks, possibly due to the absence of vectors or intermediate hosts within cities. It was higher in urban adapter species, even within a given forested habitat, emphasizing the important role of specific life-history traits. Pathogen community structure was shaped by forest anthropization and host ecology, with marked differences between urban and rural forested environments and between urban adapter and dweller species within forested urban parks. The seroprevalence of key pathogens (e.g., <i>Bartonella</i>, <i>Orthopoxvirus</i>, <i>Neoehrlichia mikurensis</i>, and Sarcocystidae) showed spatial, temporal, and host-specific variation. Epidemiological differences between sites often exceeded those between habitat types, in particular when comparing protected and managed forests, highlighting the importance of local ecological context. Nevertheless, some patterns reflected the influence of forest anthropization and species urban adaptation strategies for certain zoonotic agents. High anthropization in forests was associated with elevated <i>Bartonella</i> prevalence, driven by urban-adapter species rather than forest dwellers, emphasizing local ecological interactions between hosts and pathogens. Besides, higher levels of <i>Orthopoxvirus</i> seroprevalence were associated with adapter species in protected forests where they might be more abundant. Altogether, these findings underscore the need for integrated and multipathogen wildlife surveillance to anticipate and mitigate disease risks at the human-environment-animal interface.</p>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2026 ","pages":"4038648"},"PeriodicalIF":3.0,"publicationDate":"2026-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13136594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Gut Microbiomes in Parasitic Roundworms Reveals Phylogeny-Associated Community Structure and Functional Adaptation.","authors":"Xinyi Fan, Xuan Zhou, Lidan Wang, Xinhui Zhang, Yuanyuan Shen, Yating Xiao, Hui Wang, Lei Deng, Yue Xie","doi":"10.1155/tbed/2764696","DOIUrl":"https://doi.org/10.1155/tbed/2764696","url":null,"abstract":"<p><p>Roundworm nematodes are globally distributed zoonotic parasites that inhabit the intestinal tract of various mammals. Although these parasites reside in the host's guts, their own intestinal ecosystems remain poorly understood. Recent evidence suggests that helminths may harbor distinct gut microbiomes that contribute to their physiology and host interactions, yet cross-species comparisons are lacking. Here, we performed full-length 16S rRNA sequencing to characterize and compare the gut microbiomes of four major roundworm species-<i>Ascaris suum (As)</i>, <i>Baylisascaris schroederi (Bs)</i>, <i>Toxocara cati (Tc)</i>, and <i>Toxocara vitulorum (Tv)</i>. Across 38 individual worms, we identified 359 bacterial taxa dominated by Enterobacteriaceae, with <i>Escherichia coli</i>, <i>Salmonella enterica</i>, and <i>Klebsiella pneumoniae</i> forming a conserved core community. Despite this compositional similarity, beta-diversity and hierarchical clustering analyses revealed that microbial community structure was primarily determined by parasite phylogeny and roundworm sex, not host diet. Functional prediction using PICRUSt2 indicated clear species-specific enrichment in metabolic pathways, such as carbohydrate metabolism in <i>Bs</i> and xenobiotic metabolism in <i>As</i>, reflecting adaptive divergence of microbial functions. Collectively, these findings demonstrated that roundworm gut microbiomes exhibited taxonomic conservation but functional specialization, shaped by the evolutionary history of the parasites themselves. This study established a conceptual framework viewing the parasite as the primary host of its microbiome and provided new insights into the co-evolutionary relationships between helminths and their symbiotic bacteria.</p>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2026 ","pages":"2764696"},"PeriodicalIF":3.0,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13129500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A RNase H2-Linked TaqMan-MGB Quantitative Real-Time PCR Assay for Differential Detection of <i>Mycoplasma hyopneumoniae</i> Live-Attenuated Vaccine Strains.","authors":"Kangyao Peng, Yanna Wei, Yuzi Wu, Ting Yuan, Jiahao Li, Ping Jiang, Qingyun Xie, Zhixin Feng","doi":"10.1155/tbed/5823134","DOIUrl":"https://doi.org/10.1155/tbed/5823134","url":null,"abstract":"<p><p><i>Mycoplasma hyopneumoniae</i> (<i>M. hyopneumoniae</i>), the etiological agent of porcine enzootic pneumonia, is one of the most prevalent and economically significant respiratory pathogens in the swine industry. Vaccination constitutes a primary strategy for controlling <i>M. hyopneumoniae</i> infections. Live-attenuated vaccines confer protection against wild-type <i>M. hyopneumoniae</i> through competitive exclusion of early lung colonization and induce multifaceted immune responses, including humoral, mucosal, and cellular immunity, thereby exhibiting superior protective efficacy. However, the lack of methods for differentiating live-attenuated vaccine immunization from wild-type infection has hindered the widespread application of live-attenuated vaccines. In this study, we developed a quantitative real-time polymerase chain reaction (qPCR) method based on the ribonuclease H2 (RNase H2) cleavage principle, which emits specific fluorescent signals exclusively when the probe is fully complementary to the template of the live-attenuated vaccine strains. This qPCR method exhibited high specificity and sensitivity for the <i>M. hyopneumoniae</i> live-attenuated vaccines (168-L and RM48), with a lower detection limit of 10 copies/µL. Using this qPCR method, the bacterial shedding dynamics of the 168-L vaccine strain in piglets were effectively monitored, with the first detection in the nasal cavity at 3 days postvaccination (dpv), and a peak detection rate of 100% between 10 and 17 dpv. This established assay can specifically monitor the respiratory colonization and shedding of the vaccine strain after <i>M. hyopneumoniae</i> live-attenuated vaccine immunization, thereby providing crucial technical support for the clinical evaluation of immunization efficacy.</p>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2026 ","pages":"5823134"},"PeriodicalIF":3.0,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13129502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroepidemiological Investigation and Risk Factors of Schmallenberg Virus Infection in Sheep and Goats in Bangladesh.","authors":"Ariful Islam, Md Abu Sayeed, Monjurul Islam, Md Kaisar Rahman, Khondoker Shahriar Islam, Hameem Mollick Meem, Josefina Abedin, Anowar Hossen, Abdul Ahad, Shariful Islam, Jade K Forwood","doi":"10.1155/tbed/5788478","DOIUrl":"https://doi.org/10.1155/tbed/5788478","url":null,"abstract":"<p><p>Schmallenberg virus (SBV) is an emerging vector-borne pathogen that significantly impacts the health and productivity of both domesticated and wild ruminants, leading to considerable economic losses. Its transmission via arthropod vectors raises concerns about potential geographic expansion, particularly to South Asia, including Bangladesh, where livestock farming plays a vital role in rural livelihoods. Hence, this study aimed to estimate the serological evidence of SBV exposure and identify associated risk factors in small ruminants in Bangladesh from January 2017 to June 2019. Individual animal characteristics were recorded using a structured questionnaire. A total of 517 serum samples were collected from randomly selected goats (<i>n</i> = 230) and sheep (<i>n</i> = 287) across three districts: Dhaka, Chattogram, and Faridpur, representing both market and household settings. Serum samples were screened using a commercial indirect multi-species ELISA to detect antibodies against the SBV nucleoprotein. The overall seroprevalence of SBV was 19.5% (101/517, 95% CI: 16.2-23.2), with sheep showing higher seropositivity at 30.0% (86/287, 95% CI: 24.7-35.6) compared to goats at 6.5% (15/230, 95% confidence interval [CI]: 3.7-10.5). In a multivariable logistic regression model, sheep had significantly higher odds of SBV seropositivity than goats (odds ratio [OR]: 6.4; 95% CI: 3.6-12.2; <i>p</i>  < 0.01). Animals drinking from pond water sources also had a greater risk than those using supplied water (OR: 2.3; 95% CI: 1.2-4.5; <i>p</i> = 0.01). This study provides the first serological evidence of SBV exposure in small ruminants in Bangladesh. The findings underscore the need for targeted surveillance and improved biosecurity and management practices to reduce the risk of SBV transmission in the region.</p>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2026 ","pages":"5788478"},"PeriodicalIF":3.0,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13130852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Evaluation of Conserved Subunit Vaccine Candidates Conferring Cross-Serotype Protection Against <i>Streptococcus suis</i> Serotypes 2, 7, 8, and 9.","authors":"Shun Kang, Hongkun Zhuang, Zeren Peng, Liye Chen, Jinlu Zhu, Zongfu Wu","doi":"10.1155/tbed/3394193","DOIUrl":"https://doi.org/10.1155/tbed/3394193","url":null,"abstract":"<p><p><i>Streptococcus suis</i> is a major zoonotic pathogen with substantial serotype diversity and remains a serious threat to global swine production and public health. Accordingly, vaccines capable of inducing cross-serotype protection are urgently needed. Here, we integrated comparative genomics, bioinformatics, and reverse vaccinology to identify five conserved candidate antigens from four clinically important <i>S. suis</i> serotypes (2, 7, 8, and 9), designated rP1-rP5. The previously reported antigen PstB was included as a reference control (rP6), as it has demonstrated protective efficacy against serotypes 2, 7, and 9. All six recombinant proteins were successfully expressed and purified, and in silico analyses supported their predicted antigenic features and immunogenic potential. Following three immunizations in mice, all candidates elicited robust antigen-specific IgG responses. In opsonophagocytic assays, antisera against rP1, rP3, rP4, and rP6 displayed functional activity against serotypes 2, 7, 8, and 9. In a mouse infection model, vaccination with rP3, rP4, and rP6 reduced bacterial burdens in blood and multiple organs and conferred partial protection against all four serotypes, with rP4 producing a more pronounced reduction in tissue bacterial burdens than rP3 and rP6. Overall, rP4 and rP6 exhibited the most consistent cross-serotype protection in both bacterial burden and survival analyses, whereas rP3 showed protective efficacy, particularly against serotype 2, and may serve as a useful component of a multivalent formulation. Collectively, these findings support the rational design of broadly protective, multicomponent subunit vaccines against <i>S. suis</i>.</p>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2026 ","pages":"3394193"},"PeriodicalIF":3.0,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Isolation of Bovine Coronavirus From Yanbian, China, and Analytical Validation of a SYBR Green I RT-qPCR Panel for Calf Diarrhea Viruses.","authors":"Siqi Zhang, Haoyuan Ma, Jiawei Zhao, Kai Yu, Jingrui Hao, Hao Yu, Jianyou Jin, Shuoning Cao, Xinpeng Ji, Shujiang Xue, Qiang Li, Zhiqiang Xu, Shengwei Ji, Chenghui Li, Zheng Sun, Jialiang Xie, Rui Du, Xu Gao","doi":"10.1155/tbed/6648536","DOIUrl":"https://doi.org/10.1155/tbed/6648536","url":null,"abstract":"<p><p>Bovine coronavirus (BCoV) is an important viral agent associated with neonatal calf diarrhea (NCD), winter dysentery, and bovine respiratory disease, contributing to economic losses in cattle production worldwide. To date, BCoV sequence data from Yanbian, Northeast China, are limited in public datasets. In this study, we report the first successful isolation of a BCoV strain (BCoV-YBYJ) from a diarrheic calf in Yanbian using MDBK cells and characterize its replication in vitro and describe exploratory colon and lung findings following high-dose oral exposure in a murine model. The isolate replicated efficiently in MDBK cells. In mice, viral RNA was detected in the colon and lung with mild-to-moderate histological changes under high-dose exposure (nonnatural host). Phylogenetic analysis based on partial ORF1a, S, and M sequences showed that BCoV-YBYJ clustered with contemporary Chinese isolates within the available reference set, supporting regional genetic relatedness based on partial loci. In parallel, three SYBR Green I-based RT-qPCR assays targeting BCoV, bovine rotavirus (BRV), and bovine parvovirus (BPV) were established and analytically validated. These assays demonstrated satisfactory analytical sensitivity, specificity, and reproducibility within the validated dynamic range, and BCoV RNA was detected in 70% (140/200) of targeted diarrheic calf specimens (<i>n</i> = 200). This study provides a Yanbian BCoV isolate and analytically validated RT-qPCR assays to support differential detection and surveillance of major viral agents associated with calf diarrhea. Because these were targeted submissions from diarrheic calves, this proportion is reported descriptively and should not be interpreted as population prevalence. The BRV and BPV assays underwent analytical validation only and require further clinical evaluation before routine diagnostic application.</p>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2026 ","pages":"6648536"},"PeriodicalIF":3.0,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13121856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concomitant Infection With Porcine circovirus 2 and Porcine Epidemic Diarrhea Virus Does Not Increase Enteritis Severity in Experimentally Infected Weaned Piglets.","authors":"Priscilla F Gerber, Jianqiang Zhang, Andrew Noel, Bin Wang, Yaowei Huang, Patrick G Halbur, Tanja Opriessnig","doi":"10.1155/tbed/2698648","DOIUrl":"https://doi.org/10.1155/tbed/2698648","url":null,"abstract":"<p><strong>Background: </strong>Young pigs are often coinfected with porcine circovirus 2 (PCV2) and porcine epidemic diarrhea virus (PEDV). This study aimed to determine the impact of coinfection of pigs with PCV2 and PEDV. Forty 16-day-old crossbred mixed-sex piglets were assigned to four groups (<i>n</i> = 10/group, NEG-CONTROL, PCV2-CONTROL, PCV2+PEDV, and PEDV-CONTROL). At day postinoculation (dpi) 0, NEG-CONTROL pigs were inoculated with saline, PCV2-CONTROL pigs were inoculated with PCV2, PCV2+PEDV pigs were inoculated with PCV2 and PEDV, and PEDV-CONTROL pigs were inoculated with PEDV.</p><p><strong>Results: </strong>No clinical signs were observed in the NEG-CONTROL and PCV2-CONTROL group pigs throughout termination of the study at dpi 21. Other than mild to moderate diarrhea, which lasted for about 7 days, no other clinical signs associated with PEDV or PCV2 infection were observed in the PEDV-CONTROL and PEDV+PCV2 groups.</p><p><strong>Conclusion: </strong>Coinfection of PEDV and PCV2 had no effect on virus shedding, serum antibody profile, and macroscopic or microscopic lesions.</p>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2026 ","pages":"2698648"},"PeriodicalIF":3.0,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}