Transboundary and Emerging Diseases最新文献

{"title":"The Tiny Epigenetic Addition Plays Big Roles: The RNA Methylation in Both Human and Animal Herpesvirus Infection","authors":"Xiangqi Qiu,&nbsp;Jiajing Tian,&nbsp;Xuyang Zhao,&nbsp;Lucai Wang,&nbsp;Lele Wang,&nbsp;Yilin Bai,&nbsp;Aijun Sun,&nbsp;Guoqing Zhuang","doi":"10.1155/tbed/8542827","DOIUrl":"https://doi.org/10.1155/tbed/8542827","url":null,"abstract":"<div>\u0000 <p>During human herpesvirus infection, dynamic alterations of N6-methyladenosine (m⁶A) modification have been extensively observed in viral and cellular transcriptomes. This modification plays a crucial role in RNA metabolism, serving as a novel regulator of gene expression alongside DNA and protein modifications. Notably, reversible changes in a single m⁶A modification site can impact viral replication and pathogenicity. Recent studies have reported changes in m⁶A modification-associated epitranscriptomes and their functional analysis during animal herpesvirus infections. This review focuses on the research progress of m⁶A modification on the transcriptome in both human and animal herpesvirus infections within the same family. Specifically, it examines the dynamic alterations of m⁶A modification-associated epitranscriptomes, the expression of m⁶A-machinery proteins, regulatory molecular mechanisms associated with herpesvirus infection, and potential clinical applications. By addressing the gaps in research on m⁶A modification in animal viruses, new insights into the regulatory molecular mechanisms of viral diseases may be uncovered. Furthermore, natural hosts infected with animal herpesvirus serve as valuable biomedical models for studying the regulation of m⁶A modification on viral replication and pathogenesis, thereby supporting the development of novel vaccine and drug targets.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2025 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/8542827","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144751630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Propagation and Sequencing of African Swine Fever Virus on Porcine-Derived Buffy Coat Fraction Cells","authors":"Jessica Mason,&nbsp;Mariceny Zurita,&nbsp;Lauren Martignette,&nbsp;Janine Simmons,&nbsp;John G. Neilan,&nbsp;Michael Puckette","doi":"10.1155/tbed/1160908","DOIUrl":"https://doi.org/10.1155/tbed/1160908","url":null,"abstract":"<div>\u0000 <p>African swine fever (ASF) has emerged as a preeminent threat to worldwide pork production. Research and diagnostics of ASF virus (ASFV) is dependent upon culturing virus in primary cells, such as peripheral blood macrophages (PBMC) derived from swine blood, or pulmonary alveolar macrophages (PAM) extracted from swine lungs. The methodologies for production of these cells can be laborious, requiring significant investment in vivarium, personnel, and technical resources. As an alternative, the buffy coat cell fraction from blood contains a mixture of cell types, including undifferentiated monocytes that can be easily isolated by centrifugation. Herein, we culture buffy coat cells in macrophage (M∅) base media, containing L929 conditioned media to induce monocyte differentiation and enhance sensitivity to ASFV. Culturing the buffy coat cell fraction in M∅ base media enhanced the abundance of rosettes and number of detectable ASFV genome copies relative to buffy coat cells grown without L929 conditioned media. Buffy coat fraction cells were used to propagate ASFV directly from blood of infected swine and subsequent sequencing of extracted viral DNA yielded full genome coverage and identification of point mutations. This work demonstrated that growing ASFV in cells of the buffy coat fraction for pig blood was an effective alternative to using the traditionally isolated primary cell types for ASFV propagation, isolation, and sequencing.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2025 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/1160908","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant CA Protein-Based ELISA for Serological Detection of Small Ruminant Lentiviruses Antibodies","authors":"Xiaohua Ma,&nbsp;Yonghong Liu,&nbsp;Bofang Duan,&nbsp;Hua Gao,&nbsp;Qixin Huang,&nbsp;Cheng Chen,&nbsp;Ming Nie,&nbsp;Zhenjie Zhang,&nbsp;Zhihong Wu,&nbsp;Kui Guo,&nbsp;Zhe Hu,&nbsp;Cheng Du,&nbsp;Xiaojun Wang,&nbsp;Xue-Feng Wang","doi":"10.1155/tbed/6696495","DOIUrl":"https://doi.org/10.1155/tbed/6696495","url":null,"abstract":"<div>\u0000 <p>Maedi-visna (MV) and caprine arthritis encephalitis (CAE) are important viral diseases of sheep and goats. The diseases are caused by a group of genetically closely related lentiviruses known as small ruminant lentiviruses (SRLV), and are collectively referred to as SRLV infections. As the majority of sheep and goats infected with SRLV are asymptomatic, the disease is often overlooked. However, SRLV infection can significantly reduce animal productivity and impede animal trade. Currently, SRLV infection is widespread worldwide, but knowledge of its prevalence in China is limited due to a lack of cost-effective testing. In this study, we successfully developed an indirect enzyme-linked immunosorbent assay (iELISA) based on the SRLV capsid protein (CA) (p28) for the specific detection of anti-SRLV antibodies in serum. Using the application of checkerboard titration to optimize the iELISA assay conditions, the cut-off value was determined to be 0.09 by analyzing S/P values of 181 negative sera against SRLV that were confirmed with western blotting (WB). The method showed good reproducibility, with intra- and inter-assay coefficients of variation (CV) values less than 6.60%. A specificity test showed that the iELISA had no serological cross-reaction with six common small ruminant pathogens. Moreover, it was found to be 400–1600 times more sensitive than the available AGID test. The 93 clinical serum samples that tested SRLV-positive using the iELISA were all confirmed as positive using WB, indicating that the method has a low false positive rate. The iELISA was then applied to assess 4786 clinical serum samples from 13 cities in six provinces in China. The results show that the SRLV positivity rate of sera ranged between 0.85% and 40.00%, with the overall seroprevalence of SRLV being 10.64%. Our results indicate that the developed iELISA will serve as a valuable and efficient screening tool for the large-scale preliminary surveillance and monitoring of SRLV infections in both sheep and goats.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2025 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/6696495","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyalomma marginatum in Europe: The Past, Current Status, and Future Challenges—A Systematic Review","authors":"Seyma S. Celina,&nbsp;Jiří Černý","doi":"10.1155/tbed/7771431","DOIUrl":"https://doi.org/10.1155/tbed/7771431","url":null,"abstract":"<div>\u0000 <p><i>Hyalomma marginatum</i> is a prominent tick vector responsible for transmitting various pathogens, including the Crimean–Congo hemorrhagic fever virus (CCHFV), across Europe. This systematic review consolidates findings from 144 publications regarding the geographical distribution of <i>H. marginatum</i> and its associated pathogens. Significant populations have been identified primarily in Southern Europe and Balkan Peninsula, indicating a concerning trend. Additionally, climate change and migratory bird movements may facilitate its further dispersal, potentially leading to the establishment of <i>H. marginatum</i> in transalpine regions. Consequently, robust monitoring and surveillance strategies are essential to mitigate the public health and livestock threats posed by <i>Hyalomma</i>-borne diseases. Raising awareness and implementing preventive measures will be crucial in addressing the challenges associated with this tick vector.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2025 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/7771431","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral and Faecal Viromes of New Zealand Calves on Pasture With an Idiopathic Ill-Thrift Syndrome","authors":"Rebecca M. Grimwood,&nbsp;Jessica A. Darnley,&nbsp;John P. O’Connell,&nbsp;Hayley Hunt,&nbsp;Harry S. Taylor,&nbsp;Kevin E. Lawrence,&nbsp;Michaela B. W. Abbott,&nbsp;Ruy Jauregui,&nbsp;Jemma L. Geoghegan","doi":"10.1155/tbed/7737989","DOIUrl":"https://doi.org/10.1155/tbed/7737989","url":null,"abstract":"<div>\u0000 <p>Since 2015, an idiopathic ill-thrift syndrome featuring diarrhoea and, in some cases, gastrointestinal ulceration has been reported in weaned New Zealand dairy calves. Similar syndromes have been described in the British Isles and Australia, but investigations in New Zealand have yet to identify a specific cause. Notably, the viromes of affected calves remain understudied. We conducted metatranscriptomic analyses of oral and faecal viromes in 11 calves from a dairy farm in Taranaki, New Zealand, experiencing an outbreak of this syndrome. This included nine calves showing clinical signs. Our analysis identified 18 bovine-associated viruses across two DNA and three RNA viral families, including six novel species. Oral viromes were dominated by <i>Pseudocowpox virus</i>, which was detected in all calves with oral lesions. Faecal viromes were more diverse, featuring adenoviruses, caliciviruses, astroviruses and picornaviruses. <i>Bovine bopivirus</i>, from the <i>Picornaviridae</i> family and previously unreported in New Zealand, was significantly associated with calves showing oral lesions and diarrhoea, indicating a possible link to disease, though its role remains unclear. The diverse viral communities of the calves complicate the identification of a single causative agent. Importantly, no novel viruses were significantly associated with the syndrome, and the viromes closely resembled those found in cattle globally. These findings suggest the syndrome likely has a multifactorial origin involving nutritional, management and environmental factors rather than being driven primarily by known or novel viruses. Further, research across regions and seasons is recommended to clarify the role of viruses in idiopathic ill-thrift among New Zealand calves.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/7737989","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Full-Genome Analysis and Pathogenicity of a Novel Recombinant Field PRRSV-1 Strain Between Amervac-Like and NMEU09-1-Like Strains in China","authors":"Chunhua Wei,&nbsp;Wenlin Huang,&nbsp;Yuan Yang,&nbsp;Feimin Xie,&nbsp;Kewei Fan,&nbsp;Hua Wang,&nbsp;Haoyu Chen,&nbsp;Xin Lan,&nbsp;Ailing Dai,&nbsp;Manlin Luo,&nbsp;Xia Zhou,&nbsp;Jiankui Liu","doi":"10.1155/tbed/9925338","DOIUrl":"https://doi.org/10.1155/tbed/9925338","url":null,"abstract":"<div>\u0000 <p>Porcine reproductive and respiratory syndrome virus (PRRSV) is a major threat to the global swine industry, causing significant economic losses. In recent years, PRRSV-1 has become a growing concern in Chinese pig herds, with an increasing number of clinical infections being reported. However, the pathogenic potential of recombinant PRRSV-1 strains in piglets remains unclear. In this study, a novel recombinant PRRSV-1 strain, PRRSV1/CN/FJFQ-1/2023, was isolated in Fujian Province in 2023, and its pathogenicity for piglets was analyzed. The complete genome of PRRSV1/CN/FJFQ-1/2023 was 15,071 nucleotides (nts) excluding the poly (A) tails, and it had the highest homology (91%) with Amervac PRRS (a live vaccine virus of PRRSV-1). Compared with LV and Amervac vaccine virus, PRRSV1/CN/FJFQ-1/2023 showed distinct genetic features, including a continuous 8-amino acid (aa) deletion in the overlapping region of the ORF3 and ORF4 genes and a unique aa deletion (aa12) in the ORF7 gene. Phylogenetic analysis revealed that PRRSV1/CN/FJFQ-1/2023 belonged to Amervac-like isolates of subtype 1 of PRRSV-1, while recombination analysis showed that it was a recombinant virus from Amervac-like and NMEU09-1-like strains. Animal experiments showed that PRRSV1/CN/FJFQ-1/2023 could induce 100% morbidity, with obvious clinical symptoms, high viremia, and reduced weight gain, without death. In addition, the PRRSV1/CN/FJFQ-1/2023 caused moderate lung consolidation and interstitial pneumonia. Our results suggest that PRRSV1/CN/FJFQ-1/2023 is a moderately pathogenic strain in piglets.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/9925338","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Carcass Detection Delays on the Sustained Transmission of African Swine Fever Among Wild Boars","authors":"Dae Sung Yoo,&nbsp;Ho-Seong Cho,&nbsp;Yeonsu Oh","doi":"10.1155/tbed/9889895","DOIUrl":"https://doi.org/10.1155/tbed/9889895","url":null,"abstract":"<div>\u0000 <p>African swine fever (ASF) is a devastating infectious disease caused by the ASF virus (ASFV), a member of the <i>Asfaviridae</i> family, which primarily affects species within the Suidae family, including several African wild boars, such as the warthog (<i>Phacochoerus Africanus</i>). ASFV is characterized by its robust double-stranded DNA genome and is transmitted through various transmission routes, including direct contact with infected pigs or fomites, ingestion of contaminated swill, and ticks from the <i>Ornithodoros</i> genus. Upon entry, the virus induces cell apoptosis, systemic hemorrhage, and high fever, typically leading to a near 100% fatality rate among affected pigs, thereby causing substantial losses to global swine populations. Similar to ASF outbreaks in other countries, South Korea reported its initial ASF infections in domestic pig farms in September 2019, following an incursion in wild boar populations. Subsequently, the virus propagated southward, tracing the natural migratory paths of wild boars through forested and mountainous regions, and sporadically infecting nearby pig farms. In response, robust surveillance of wild boar populations has become crucial, as these efforts provide timely and essential information to stakeholders. Effective and prompt removal of ASF-infected carcasses is critical, as these carcasses can remain infectious for extended periods, thereby posing a continuous risk of secondary outbreaks. This study conducts a comprehensive analysis of the spatiotemporal patterns of ASF-infected wild boar carcasses, based on 41,192 wild boar samples collected through active and passive surveillance from September 2019 to March 2022. It examines how environmental conditions, such as forest density, regional humidity, and geographical terrain impact carcass decomposition rates and consequently, the detection timelines of ASF-infected wild boars. This research aims to pinpoint factors contributing to detection delays and refine strategies for early detection and rapid removal of contaminated carcasses, thereby enhancing control measures and mitigation efforts against ASF in affected regions.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/9889895","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sporadic Human Infections With Rickettsia japonica in Yichang, China, 2021–2023","authors":"Yuting Ren,&nbsp;Yale Jiang,&nbsp;Zhoufu Xiang,&nbsp;Qi Cheng,&nbsp;Kehan Chen,&nbsp;Jianchun Ma,&nbsp;Jianwei Dai,&nbsp;Weihao Zhang,&nbsp;Wei Hou,&nbsp;Qiang Liu,&nbsp;Liangjun Chen","doi":"10.1155/tbed/4832524","DOIUrl":"https://doi.org/10.1155/tbed/4832524","url":null,"abstract":"<div>\u0000 <p>Japanese spotted fever (JSF) is an easily neglected infectious disease, where misdiagnosis and delayed treatment significantly contribute to poor prognoses in affected patients. Our prospective observational study (2021–2023) systematically characterized 56 JSF cases in Yichang through tripartite analysis encompassing epidemiological distributions, clinical phenotyping, and phylogenetic relationship analysis of <i>Rickettsia japonica</i> (<i>R. japonica</i>). Our study delineated distinct clinical presentations of JSF, and identified five laboratory indexes demonstrating significant associations with disease severity. Notably, thrombocytopenia (platelet deficiency) and procalcitonin (PCT) levels emerged as critical indicators, with PCT as a well-characterized inflammatory mediator showing particular prognostic value for anticipating severe complications in rickettsial infections, consistent with prior pathophysiological research. Phylogenetic analysis revealed that <i>R. japonica</i> strains distributed in Yichang City exhibited extremely low genomic diversity. Further, the <i>R. japonica</i> strains isolated in our study exhibited a high degree of homology with <i>R. japonica</i> isolation within the borders of China. In summary, our research identified several factors that indicate a high risk of poor outcomes in <i>R. japonica</i> infections. Additionally, we observed highly similar phylogenetic relationships among <i>R. japonica</i> strains, which have important implications for disease prevention, control, and clinical diagnosis.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/4832524","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, Genetics, and Evolution of Porcine Astrovirus 3 in China","authors":"Qingqing Wu,&nbsp;Jianfeng Jiang,&nbsp;Qingxian Li,&nbsp;Chaoting Xiao,&nbsp;Baoyu Chen,&nbsp;Mingkai Sun,&nbsp;Jiachao Xu,&nbsp;Shijiang Mi,&nbsp;Biao He,&nbsp;Changchun Tu,&nbsp;Wenjie Gong","doi":"10.1155/tbed/3170440","DOIUrl":"https://doi.org/10.1155/tbed/3170440","url":null,"abstract":"<div>\u0000 <p>Porcine astrovirus 3 (PAstV3) is an emerging virus associated with porcine polioencephalomyelitis outbreaks and is frequently detected in the feces of both healthy and diarrheal pigs. Despite its significance, the prevalence, genetic characteristics, and evolutionary features of PAstV3 in China remain poorly understood. Here, a retrospective study of PAstV3 infection was conducted using 634 tissue or fecal specimens collected from diseased pigs across 29 regions in China between 1990 and 2024. As a result, eight tissue samples and 32 fecal samples from seven regions tested positive for PAstV3, yielding an overall positive rate of 6.3% (40/634). Through a combination of metatranscriptomics (MTT) and RT-PCR, nearly complete genome sequences of 14 PAstV3 strains and full-length open reading frame (ORF) 2 sequences of another 13 PAstV3 strains were successfully obtained. Sequence analysis revealed that these strains exhibited a sequence identity of 59.8%–100% in the capsid protein. Phylogenetic analysis demonstrated that global PAstV3 strains could be classified into four genotypes: PAstV3a–d. The intergenotype strains showed nucleotide (nt) and amino acid sequence identities of 59.7%–69.8% and 59.5%–72.0%, respectively, in the ORF2 region. Notably, PAstV3b was identified as the predominant genotype in China. Sequence comparisons further confirmed the close genetic relationship between PAstV3 and neurotropic mammalian astroviruses, suggesting the potential interspecies transmission or a shared evolutionary origin. Phylodynamic analysis estimated that the most recent common ancestor (tMRCA) of PAstV3 emerged around the year 1660 and the effective population size of PAstV3 has been continuously expanding over time.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/3170440","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic Identification of a Novel Zoonotic Gemykibivirus in a Diarrheic Pig in China","authors":"Wenqiang Wang,&nbsp;Lin Yuan,&nbsp;Miaojie Zhang,&nbsp;Qilin Zhao,&nbsp;Wenqiang Pang,&nbsp;Dehu Sun,&nbsp;Xiaoye Jia,&nbsp;Feifei Tan,&nbsp;Tingting Niu,&nbsp;Kegong Tian,&nbsp;Xiangdong Li","doi":"10.1155/tbed/7560012","DOIUrl":"https://doi.org/10.1155/tbed/7560012","url":null,"abstract":"<div>\u0000 <p>Gemykibiviruses are circular, replication-associated protein (REP) encoding single-stranded DNA (ssDNA) viruses classified within the genus <i>Gemykibivirus</i> of the family Genomoviridae. In recent years, several gemykibiviruses have been detected in humans presenting with clinical symptoms, such as encephalitis, respiratory illness, sepsis, pericarditis, diarrhea, and multiple sclerosis. However, the presence of gemykibiviruses in other animal hosts, as well as the evolution of the <i>Gemykibivirus</i> genus, remains poorly understood. In this study, we identified a novel gemykibivirus from a diarrheic pig in China using metagenomic sequencing, which we designated as pGkV. The pGkV genome comprises 2200 nucleotides and encodes two key proteins as follows: the capsid-associated protein (CAP) and the REP. Phylogenetic analyses revealed that pGkV clusters within the zoonotic gemykibivirus (ZooGkV) clade, which includes nearly all gemykibivirus species recently identified in humans. Mutation and haplotype analyses revealed that pGkV is closely related to an avian gemykibivirus, while its CAP and REP proteins are identical to those of a human gemykibivirus, underscoring its potential zoonotic capability. Furthermore, recombination signals were detected among Zoo-GkVs, suggesting that recombination may contribute to the emergence of novel gemykibivirus strains. The identification of pGkV provides new insights into the evolution and cross-species transmission of gemykibiviruses.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/7560012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}