Transboundary and Emerging Diseases最新文献

{"title":"High Prevalence of a Novel Circovirus in the European Hedgehog (Erinaceus europaeus), a Common Species in Decline","authors":"Kevin P. Mulder,&nbsp;Frank Pasmans,&nbsp;Filip van Nieuwerburgh,&nbsp;Naomi Terriere,&nbsp;Moira Kelly,&nbsp;Seline Bregman,&nbsp;Elin Verbrugghe,&nbsp;An Martel","doi":"10.1155/2024/4670252","DOIUrl":"https://doi.org/10.1155/2024/4670252","url":null,"abstract":"<div>\u0000 <p>Hedgehog (<i>Erinaceus europaeus</i>) declines in western Europe have been associated with the emergence of Hedgehog diphtheric disease (HDD), with a probable multifactorial, yet unidentified etiology. We used metagenomic sequencing of cell-free DNA (cfDNA) in hedgehog blood to identify possible causes of HDD. We detected a novel circovirus species in the European hedgehog, providing the first record of a circovirus within the mammalian order Eulipotyphla. The novel circovirus genome exhibits the characteristic circovirus structure, including a functional replicase (REP) and capsid (CAP) gene. Phylogenetic analysis placed all four detected genomes in a monophyletic clade, most closely related to sequences isolated from dogs. Subsequent PCR-based screening of 188 hedgehog liver samples demonstrated a high prevalence (61%) of this circovirus in hedgehogs brought to wildlife rescue centers, however, without any significant association with HDD. Since circoviruses are well known to interfere with host immunity across mammalian and avian taxa, the high level of circovirus detection in hedgehogs warrants further research into the role of this novel virus in hedgehog health.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/4670252","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Genetic Analysis of Species D Rotaviruses in Pangolin Samples","authors":"Kai Wang,&nbsp;Shasha Liu,&nbsp;Xiaotong Liang,&nbsp;Wanke Hu,&nbsp;Zhenyu Wen,&nbsp;Jiayi Wang,&nbsp;Xianghe Wang,&nbsp;Fuyu An,&nbsp;Ziqiao Chen,&nbsp;Haikuo Yan,&nbsp;Hongmei Yan,&nbsp;Lei Wang,&nbsp;Xiaoai Zhang,&nbsp;Jieshi Yu,&nbsp;Wen-Kang Wei,&nbsp;Yan Hua","doi":"10.1155/tbed/1773821","DOIUrl":"https://doi.org/10.1155/tbed/1773821","url":null,"abstract":"<div>\u0000 <p>Pangolins have been found to carry severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-related coronaviruses. In light of this discovery, interest has been piqued in viromes of these heavily trafficked wild animals. In this study, we performed viral metagenomic sequencing to explore viromes of both confiscated dead pangolins and captive healthy pangolins. Sequence reads of vertebrate-associated viruses in <i>Herpesviridae</i>, <i>Retroviridae</i>, <i>Iridoviridae</i>, <i>Reoviridae</i>, <i>Arenaviridae</i>, and <i>Flaviviridae</i> were detected in confiscated dead pangolins. A novel rotavirus (RV) (<i>Reoviridae</i>), showing a high degree of genetic similarity to the RV species D (RVD) that was previously unreported in mammals, was further confirmed by using reverse transcription-polymerase chain reaction (RT-PCR) and Sanger sequencing. Three out of 18 samples from the confiscated dead pangolins were positive for genomic sequences of the novel RV. Importantly, sequence alignments and phylogenetic analyses demonstrated that these RV strains genetically belonged to the RVD. Nevertheless, these novel RVD strains were divergent from known RVD strains that have been found only in Avian. They formed a separate genetic cluster. Five serial passages were attempted to isolate the RV, but no live virus was obtained. In addition, fecal samples were collected from healthy pangolins (<i>n</i> = 41) in our institution and screened for RVs by viral metagenomic sequencing and RT-PCR. In these fecal samples, neither species D nor previously identified species A RVs were detected. This study reported RVDs in pangolin samples for the first time to our knowledge. Identifiability disagreements between wild and captive pangolins highlight the need for further exploration into pangolin viruses to better understand their emergence and transmission potential.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/1773821","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rotaviruses in Pigeons With Diarrhea: Recovery of Three Complete Pigeon Rotavirus A Genomes and the First Case of Pigeon Rotavirus G in Europe","authors":"Ewa Łukaszuk,&nbsp;Daria Dziewulska,&nbsp;Tomasz Stenzel","doi":"10.1155/tbed/4684235","DOIUrl":"https://doi.org/10.1155/tbed/4684235","url":null,"abstract":"<div>\u0000 <p>Rotaviruses are well-recognized pathogens responsible for diarrhea in humans and various animal species, with <i>Rotavirus A</i> the most often detected and most thoroughly described. Rotaviral disease is an important concern in pathology of pigeons as well, as pigeon rotavirus A was proven to play a major role in young pigeon disease (YPD). However, rotaviruses of other groups have been so far understudied in birds. This paper describes the first finding of Rotavirus G in domestic pigeon in Europe, as well as the recovery of three complete genomes of pigeon rotavirus A with Oxford Nanopore Sequencing. Quantification of pigeon rotavirus A genetic material with droplet digital polymerase chain reaction (PCR) in pigeons suffering from diarrhea and in asymptomatic pigeons was also performed in the frameworks of this study and resulted in determination of statistically highly significant differences between the groups in both detection rate and shedding of the virus. Phylogenetic analysis revealed the close relationship of acquired strains with those originating from pigeons from Europe, North America, Asia, and Australia, indicating a broad geographical spread of pigeon rotaviruses. Results of our research shed more light on occurrence and diversity of <i>Rotavirus</i> species in pigeons.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/4684235","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SARS-CoV-2 Seroprevalence in Indoor House Cats From the Lisbon Area During the COVID-19 Pandemic, 2019–2021","authors":"Isa Moutinho,&nbsp;Mafalda Henriques,&nbsp;Sara Cardoso,&nbsp;Teresa da Penha Coutinho,&nbsp;Carlos Penha-Gonçalves,&nbsp;Jocelyne Demengeot,&nbsp;Miguel Castanho,&nbsp;Luís Tavares,&nbsp;Solange Gil,&nbsp;Telmo Nunes,&nbsp;Frederico Aires-da-Silva","doi":"10.1155/tbed/1543922","DOIUrl":"https://doi.org/10.1155/tbed/1543922","url":null,"abstract":"<div>\u0000 <p>The susceptibility of various animal species to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been studied extensively. Cats have garnered significant concern due to their high susceptibility and proximity to humans. This study aimed to evaluate the susceptibility and antibody response in house cats exposed to SARS-CoV-2 when human infection was spreading in the Lisbon area during the 2019–2021 period. A total of 733 serum samples were collected and characterized from cats admitted to the Veterinary Teaching Hospital of the Faculty of Veterinary Medicine at the University of Lisbon (HEV-FMV-ULisboa). All samples were tested by enzyme-linked immunosorbent assay (ELISA) for immunoglobulin M (IgM) and immunoglobulin G (IgG) anti-SARS-CoV-2 whole Spike and receptor-binding domain (RBD) proteins from the Wuhan-Hu-1 isolate and 14.7% (108/733) tested positive, suggesting exposure to the human virus. Surrogate virus neutralization test (sVNT) against the Wuhan-Hu-1 isolate showed that 20.4% of ELISA positive samples (22/108) harbored neutralizing antibodies against the virus. The 22 most promising serum samples were retested using ELISA and sVNT against Alpha, Delta, and Omicron SARS-CoV-2 variants. Notably, these samples exhibited antibodies that were capable of recognizing and neutralizing these variants. Subsequent neutralization assays confirmed that the serum samples effectively inhibited the infection process of Wuhan-Hu-1 D614G, Delta, and Omicron SARS-CoV-2 pseudotyped viruses. Our findings indicate that cats were exposed to SARS-CoV-2 infection during the pandemic period and generated highly effective and broadly neutralizing antibodies against the virus. Although cats have not been demonstrated to significantly contribute to the spread of SARS-CoV-2, their high susceptibility to asymptomatic infection underscores the importance of investment in preventive surveillance measures. In summary, our study reinforces the notion that cats naturally infected with SARS-CoV-2 represent a valuable anthroponotic disease model in house settings and might be a potential source for the development of antibodies against SARS-CoV-2 in tackling future outbreaks with a One Heath perspective.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/1543922","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Single-Copy Sensitive and Field-Deployable One-Pot RT-RPA CRISPR/Cas12a Assay for the Specific Visual Detection of the Nipah Virus","authors":"Kaikai Jin,&nbsp;Pei Huang,&nbsp;Boyi Li,&nbsp;Zengguo Cao,&nbsp;Zanheng Huang,&nbsp;Zimo Zhang,&nbsp;Meihui Liu,&nbsp;Hao Li,&nbsp;Lijuan Niu,&nbsp;Tianyi Zhang,&nbsp;Yuanyuan Li,&nbsp;Xuemeng Li,&nbsp;Hualei Wang,&nbsp;Haili Zhang","doi":"10.1155/2024/4118007","DOIUrl":"https://doi.org/10.1155/2024/4118007","url":null,"abstract":"<div>\u0000 <p>Nipah virus (NiV) is an emerging bat-borne zoonotic virus that can be transmitted to humans and other animals through infected bats or contaminated foods. The disease is highly lethal in humans (40%–75%) and has the potential for human-to-human transmission. Currently, there are no approved treatments or vaccines for NiV infection in humans or animals. Consequently, there is a pressing need for a highly sensitive, precise, and visually detectable assay to enable early intervention and mitigate the transmission of NiV infection. Here, we report a single-copy sensitive, field-deployable, one-pot visual reverse transcription-recombinase polymerase amplification (RT-RPA)-clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR associate system (Cas)12 for the detection of NiV. The assay works by targeting the <i>N</i> gene of NiV, and the results are directly visible to the naked eye. The assay has demonstrated the ability to detect as few as 5.5 copies/μl of positive plasmids or 5.5 × 10¹ copies/μl of RNA transcripts when reacted at constant temperature for 40 min. It showed high specificity for NiV and had no cross-reaction with other pathogens, including rabies virus (RABV), Japanese encephalitis virus (JEV), herpes simplex virus type 1 (HSV-1), Hendra virus (HeV), and <i>Streptococcus suis</i> (<i>S. suis</i>), that can cause clinical symptoms similar to those of NiV infection. Moreover, this assay had a 100% coincidence rate with the reverse transcription quantitative polymerase chain reaction (RT-qPCR) method recommended by the World Organization for Animal Health (WOAH) for the detection of simulated clinical samples, indicating that it has great potential as an ultrasensitive, simple, and portable novel assay for the onsite diagnosis of NiV infection.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/4118007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Global Distribution and Characteristics of Research Studies Focusing on Swine Farm Biosecurity: A Scoping Review","authors":"Isha Agrawal,&nbsp;Erin E. Kerby,&nbsp;Csaba Varga","doi":"10.1155/2024/6497633","DOIUrl":"https://doi.org/10.1155/2024/6497633","url":null,"abstract":"<div>\u0000 <p>Despite significant advances in swine biosecurity (BS) over the last decade, BS plans have yet to be broadly adopted on swine farms. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScRs) framework was followed to review the literature, describe the worldwide distribution of publications on swine farm BS, and characterize the research methodologies used. The final data extraction and analysis included 157 publications originating from 48 countries. Several publications (<i>n</i> = 93) used face-to-face interviews for data collection. An increase in the adoption of online and multimode approaches was detected after 2009. Many publications (<i>n</i> = 92) focussed on the impact of BS on the incidence of swine diseases such as porcine reproductive and respiratory syndrome (PRRS) and African swine fever (ASF). Only 16 studies reported proposing incentives for study participation. Regions with high publication numbers were detected in Western and Southern Europe, Northeast of South America, and East Africa. Areas with low publication numbers were in Eastern Europe, North and Central Africa, Central America, and the Northwest of South America. This study identified the most common study methodologies used to assess swine farm BS. Countries with limited swine BS research studies were identified where future investigations are needed.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/6497633","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of HPAI H5N6 Clade 2.3.4.4b in Wild Birds: A Case Study From South Korea, 2023","authors":"Chang-Gi Jeong,&nbsp;Chung-Young Lee,&nbsp;Su-Beom Chae,&nbsp;Jung-Hoon Kwon,&nbsp;Eun-Jee Na,&nbsp;Jun-Soo Park,&nbsp;Young-Sik Kim,&nbsp;Seung-Chai Kim,&nbsp;Hwan-Ju Kim,&nbsp;Young-Sun Sung,&nbsp;Sun-Young Kim,&nbsp;Won-Il Kim,&nbsp;Jae-Ku Oem","doi":"10.1155/tbed/4141478","DOIUrl":"https://doi.org/10.1155/tbed/4141478","url":null,"abstract":"<div>\u0000 <p>The emergence and evolution of avian influenza A viruses (AIVs) pose significant challenges to both public health and animal husbandry worldwide. Here, we characterized a novel reassortant highly pathogenic avian influenza virus (HPAIV), clade 2.3.4.4b H5N6, that was isolated from a mandarin duck in South Korea in December 2023. Phylogenetic and molecular analyses show that the hemagglutinin (HA) gene of the 23-JBN-F12-36/H5N6 virus clustered with HPAIV clade 2.3.4.4b H5N1 viruses, which were circulating in South Korea and Japan in 2022–2023. The M and polymerase acidic (PA) genes also revealed a close association with the HPAIV clade 2.3.4.4b H5N1 AIV that was identified previously in South Korea during November 2022. Notably, the neuraminidase (NA) gene of the 23-JBN-F12-36/H5N6 virus was estimated to have its origins in the HPAIV clade 2.3.4.4h H5N6 prevalent in poultry in China, and it is clustered with the AIVs that are associated with human infection cases. Taken together, these results show that the virus has been produced by reassortment with H5N1 HPAIV, which is prevalent in wild birds; H5N6 HPAIV, which is circulated in poultry in China; and the internal genes of low pathogenic avian influenza viruses (LPAIVs). In light of the reassortment of HPAIVs circulating in existing wild birds and HPAIVs circulating in poultry in China within the 2.3.4.4b H5Nx clade, it is imperative to strengthen active surveillance across wild bird populations, poultry farms, and live poultry markets, and to inform for the effective design of improved prevention and control strategies.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/4141478","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Syndemic Challenges: Addressing the Resurgence of Mpox Amidst Concurrent Outbreaks in the DRC","authors":"Olaniyi Abideen Adigun,&nbsp;Olalekan John Okesanya,&nbsp;Mohamed Mustaf Ahmed,&nbsp;Bonaventure Michael Ukoaka,&nbsp;Don Eliseo Lucero-Prisno III,&nbsp;Emmanuella Ogechi Onyeaghala,&nbsp;Emmanuel Ayomide Oluwasusi,&nbsp;Olamide Esther Ogunwale,&nbsp;Ayodeji Amos Faniyi","doi":"10.1155/tbed/1962224","DOIUrl":"https://doi.org/10.1155/tbed/1962224","url":null,"abstract":"<div>\u0000 <p>The Democratic Republic of Congo (DRC) faces a syndemic of infectious diseases, including monkeypox (mpox), cholera, measles, anthrax, and plague, worsening public health challenges and socioeconomic disparities. This review synthesizes and discusses epidemiological data and consequences of simultaneous outbreaks in the DRC between January 2023 and March 2024. The findings highlight a 6.7% fatality rate and 3319 confirmed cases of mpox, with significant outbreaks in Kinshasa and 22 other provinces. Anthrax occasionally surfaced among cattle-raising villages, measles affected fewer than five children susceptible to the disease, and cholera outbreaks persisted in North Kivu, South Kivu, and Tanganyika. Plague incidences, mostly bubonic, have been reported in Ituri province. Vulnerable groups, including children, mothers, the elderly, and those with compromised immune systems, face increased risks due to poor healthcare access, hunger, and underlying medical conditions. Cultural beliefs, healthcare system issues, and socioeconomic instability impede effective response tactics. This strain on the fragile healthcare system highlights the need for increased surveillance, immunization efforts, and community involvement. To mitigate the effects of syndemic outbreaks, strengthening the DRC’s health systems through international cooperation, integrated public health initiatives, and improved access to healthcare is crucial.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/1962224","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Specific Detection of RHDV GI.1 and GI.2 by RT-LAMP-CRISPR/Cas12a Platform","authors":"Mengting Wu,&nbsp;Mengmeng Chen,&nbsp;Rulong Qiu,&nbsp;Lei Ge,&nbsp;Zhiyu Fan,&nbsp;Bo Hu,&nbsp;Houjun Wei,&nbsp;Yiming Li,&nbsp;Fang Wang,&nbsp;Yanhua Song","doi":"10.1155/tbed/3881457","DOIUrl":"https://doi.org/10.1155/tbed/3881457","url":null,"abstract":"<div>\u0000 <p>Rabbit hemorrhagic disease is a highly contagious and acute fatal disease caused by rabbit hemorrhagic disease virus (RHDV). The first outbreak of RHDV2 in 2020 has posed a serious threat to the rabbit breeding industry in China. An effective and specific detection strategy for RHDV GI.1 (RHDV1) and GI.2 (RHDV2) is urgently needed. In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP)-CRISPR/Cas12a-based dual readout portable detection platform. The platform showed excellent specificity to identify RHDV1 and RHDV2 strains and no cross-reaction with other prevalent pathogens of rabbit. The detection limit for RHDV1 and RHDV2 by RT-LAMP-CRISPR/Cas12a could reach 10 copies/μl of the VP60 gene per reaction. Furthermore, 74 clinical samples were detected for both RHDV1 and RHDV2. RT-LAMP-CRISPR/Cas12a-based dual readout portable detection platform showed 25.68% (19/74) RHDV1-positive samples, 43.24% (32/74) RHDV2-positive samples, and 8.11% (6/74) RHDV1/RHDV2 double positive samples, respectively. The coincidence rates of detection RHDV1 and RHDV2 between RT-LAMP-CRISPR/Cas12a and quantitative real-time-polymerase chain reaction (qPCR) were both 97.30%. RT-LAMP-CRISPR/Cas12a showed higher sensitivity and detection rate compared with qPCR. Moreover, the results were visible to the naked eye within 1.5 h combined with lateral flow strips (LFSs) and visual fluorescence. The RT-LAMP-CRISPR/Cas12a portable platform has the advantages of high sensitivity, specificity, fast, low equipment requirements, which can be used in clinical practice in rural areas and resource-limited settings.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/3881457","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Prevalence Estimation and Phylogenetic Analysis of Novel Porcine Parvoviruses (PPV2–7) in Hungarian Pig Herds","authors":"Barbara Igriczi,&nbsp;Lilla Dénes,&nbsp;Kitti Schönhardt,&nbsp;Aleksandra Woźniak,&nbsp;Tomasz Stadejek,&nbsp;Gyula Balka","doi":"10.1155/2024/5117884","DOIUrl":"https://doi.org/10.1155/2024/5117884","url":null,"abstract":"<div>\u0000 <p>To date, seven novel parvoviruses have been identified in pigs and designated as porcine parvovirus 2–7 (PPV2–7). The presence of these emerging viruses has been reported in several countries around the world, although their pathogenic role and clinical and economical relevance are largely unknown. Here, we report the estimated prevalence and genetic diversity of novel PPV2–7 in Hungarian pig herds and the detection of these viruses in two Slovakian pig farms. For the comparative prevalence estimation, 2505 serum samples from different age groups, 218 oral fluid samples, and 111 processing fluid samples were collected from 26 large-scale Hungarian farms according to a systematic, cross-sectional sampling protocol. All samples were tested by real-time quantitative polymerase chain reaction (qPCR), and the presence of at least one PPV was detected in 24 of the 26 (92%) Hungarian and both Slovakian farms, suggesting high levels of subclinical circulation in most herds. The estimated PPV2–7 prevalence in Hungary varied from 50% to 89%, with PPV4 being the least and PPV2 being the most prevalent virus. The highest detection rates were observed in oral fluid samples, indicating that this sample type is most suitable for screening PPVs, but all viruses were also detected in serum samples and processing fluids. All novel PPVs were most frequently detected in the serum samples of weaned pigs and fatteners, with slightly higher viral burden in the younger age groups. These results may suggest an age-related susceptibility, which could play a significant role in the epidemiology of these viruses, impacting herd health and productivity.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/5117884","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}