Aquaculture International最新文献

{"title":"Influence of morphotypes and sex on the morphometry, meat yield traits, nutritional composition, fatty acid, and amino acid contents of Macrobrachium rosenbergii (De Man 1879)","authors":"George Rayventh C.,&nbsp;Chidambaram P.,&nbsp;Aanand S.,&nbsp;Vijay Amirtharaj K. S.,&nbsp;Vanathi V.,&nbsp;Parameswaran Hariharan,&nbsp;Somu Sunder Lingam R","doi":"10.1007/s10499-025-02298-w","DOIUrl":"10.1007/s10499-025-02298-w","url":null,"abstract":"<div><p>The present study examined the morphometrical characteristics, yield data, proximate composition, fatty acid, and amino acid profiles of the different male and female morphotypes of <i>Macrobrachium rosenbergii</i>. The prawn samples were collected from the grow-out ponds after a 120-day culture period, and they were segregated into four distinct groups such as small males (SM), orange claw males (OC), blue claw males (BC), and females (FE), following the standard identification methods. Out of 1000 specimens collected, the relative proportion study found a higher occurrence of small males (32.59 ± 2.18%) compared to other morphotypes and females. The morphometrical study revealed significantly higher body weight (66.59 ± 2.61 g) and dactyl length (4.33 ± 0.15 cm) in blue claw males. On the other side, the meat yield study revealed significantly higher cephalothorax weight (45.52 ± 1.81 g) and weight of the second leg (15.16 ± 1.08 g) in blue claw males, whereas tail weight percentage (49.89 ± 1.66% and 48.50 ± 0.48%) and somatic yield (96.02 ± 0.30% and 95.29 ± 0.38%) were found to be higher in females and small males, respectively. Proximate composition revealed higher levels of protein (22.23 ± 0.13% and 21.90 ± 0.25%) and lipid (5.77 ± 0.19% and 6.87 ± 0.19%) in small males and females, respectively. Upon analyzing the fatty acid profile, females had higher levels of omega-3 fatty acids (9.13 ± 0.05%), eicosapentaenoic acid (EPA) (7.84 ± 0.02%), omega-6 fatty acids (21.32 ± 0.05%), and PUFA/SFA ratio (0.72 ± 0.01). Interestingly, small males recorded higher levels of lignoceric acid (6.68 ± 0.02%). The essential amino acids, such as histidine (1.05 ± 0.03%), arginine (3.67 ± 0.10%), and methionine (0.53 ± 0.02%), were significantly higher in females. In contrast, the total amino acid level was found to be significantly higher in the small males (22.40 ± 0.65%). Overall, the findings of the present study recommend that females and small males have higher processing potential as they possess higher meat yield and better nutritional quality than other male morphotypes, which suggests that the processing industry could potentially use all morphotypes of males and females of <i>M. rosenbergii</i> for the sustainable expansion of the prawn farming industry.\u0000</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145316307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Additive and non-additive genetic effects in complete diallel cross of Crassostrea angulata strains tested in monsoon tropical farming environments","authors":"Luan Thanh Nguyen,&nbsp;Anh Mai Thi Nguyen,&nbsp;Nguyen Hoang Khoi Le,&nbsp;Suong Thao Nguyen,&nbsp;Nguyen Hong Nguyen,&nbsp;Tri Nhu Nguyen,&nbsp;Sang Van Nguyen","doi":"10.1007/s10499-025-02285-1","DOIUrl":"10.1007/s10499-025-02285-1","url":null,"abstract":"<p>As a native of subtropical regions, the Portuguese oyster (<i>Crassostrea angulata</i>) has not been extensively evaluated under monsoon tropical farming systems for breeding program. This study examined additive and non-additive genetic effects for body weight (g, total live whole weight of individual oysters) and survival rate (%, proportion of oysters alive relative to the initial stocking) in a complete 4 × 4 diallel cross using four geographically different <i>C. angulata</i> strains, tested under monsoon tropical estuarine conditions in Southern Vietnam. After 180 days of grow-out, data on body weight and survival at harvest were collected from 13,173 oysters across four sites with fluctuating salinity (14–30 ppt) and stable temperatures (29–30 °C). Our results revealed significant additive genetic variation and genotype-by-environment interactions across environment. The Vung Tau (V) and Khanh Hoa (K) strains exhibited the strongest additive genetic effects (AGE) for body weight and survival rate, with V showing consistent contributions across hybrid combinations. In contrast, Quang Ninh (Q) and Taiwan (T) strains displayed negative additive genetic performance, reflecting limited adaptation to tropical estuarine conditions. Reciprocal effects contributed to phenotypic variation and showed asymmetry in certain crosses, suggesting maternal and environmental interactions. Heterosis was generally negative or negligible for body weight, but modest positive values for survival were detected in crosses involving Q (e.g., QK, QT), indicating limited dominance effects. Collectively, additive genetic variance was the primary driver of trait improvement, underscoring the importance of strain selection. Strains V and K are promising candidates for developing a synthetic base population, while the inclusion of genetically distinct strains such as Q and T may help maintain long-term diversity in breeding programs targeting tropical oyster aquaculture.\u0000</p>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145316306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TSS-optimized biofloc (200–350 mg/L) enhances survival and antioxidant defense in Cherax quadricarinatus juveniles with reduced aggression and improved water stability","authors":"Chengxu Ruan,&nbsp;Junhao Tang,&nbsp;Jiaohong Wang,&nbsp;Ye Tao","doi":"10.1007/s10499-025-02291-3","DOIUrl":"10.1007/s10499-025-02291-3","url":null,"abstract":"<div><p>This study established three total suspended solid (TSS) concentration gradients in biofloc technology (BFT) systems: low (BFTL, ≤ 200 mg/L), medium (BFTM, 200–350 mg/L), and high (BFTH, 350–500 mg/L), with clear water (CW) as control. The 35-day culture performance of redclaw crayfish (<i>Cherax quadricarinatus</i>) juveniles (initial weight 5.58 ± 0.32 mg, density 50 ind./m²) was evaluated. Results demonstrated that the BFTM group achieved the highest survival rate, significantly surpassing the control (<i>p</i> &lt; 0.05), alongside the lowest limb loss rate and significantly narrower weight and length ranges compared to CW (<i>p</i> &lt; 0.05). Antioxidant indicators revealed that BFTM exhibited the highest activities of superoxide dismutase (SOD), catalase (CAT), and total antioxidant capacity (T-AOC), with the lowest malondialdehyde (MDA) content (<i>p</i> &lt; 0.05), indicating effective maintenance of antioxidant function in crayfish. In contrast, the BFTH group showed decreased SOD/CAT activities, elevated MDA levels, and reduced survival, likely due to elevated physiological stress associated with high floc density. Water quality analysis indicated that nitrogenous waste in BFT systems initially accumulated before stabilizing, with all water quality parameters remaining within safe ranges. In conclusion, TSS of 200–350 mg/L optimizes nursery culture by reducing aggression through turbidity-mediated concealment, simultaneously improving cohort uniformity and antioxidant capacity in redclaw crayfish juveniles.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145316265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of melatonin pretreatment on oxidative damage and immune system impairment in Eriocheir sinensis exposed to ammonia-N stress: insights from the intestine-hepatopancreas axis","authors":"Rui Wang,&nbsp;Hui Peng,&nbsp;Dapeng Li,&nbsp;Rong Tang","doi":"10.1007/s10499-025-02286-0","DOIUrl":"10.1007/s10499-025-02286-0","url":null,"abstract":"<div><p>Ammonia-N represents the most toxic nitrogen compound for crustaceans. Melatonin (MT), a natural antioxidant, is capable of efficiently mitigating oxidative damage within the body. The objective of this experiment was to evaluate the protective effect of melatonin pretreatment on the Chinese mitten crab (<i>Eriocheir sinensis</i>) under ammonia-N stress and to analyze the potential connection between the protective effect of MT and the intestine–hepatopancreas axis. A 14-day feeding trial and a 48-h acute ammonia-N stress trial were employed to explore the influence of MT feeding on the antioxidant capacity, immunity, and gut microbiota of the Chinese mitten crab. The findings demonstrated that acute ammonia-N stress disrupted the histoarchitecture of the crab’s hepatopancreas and diminished the antioxidant and nonspecific immunity of the crab. When exposed to ammonia-N subsequent to MT pretreatment, the activities of Superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (T-AOC), and glutathione peroxidase (GSH-Px) were significantly up-regulated in the hemolymph of crabs (<i>P</i> &lt; 0.05), while the malondialdehyde (MDA) level was significantly down-regulated (<i>P</i> &lt; 0.05). Moreover, in the hepatopancreas, the activities of GSH-Px and CAT were significantly up-regulated (<i>P</i> &lt; 0.05), while lysozyme (LZM) activity was significantly down-regulated (<i>P</i> &lt; 0.05). In the hemolymph, the activities of acid phosphatase (ACP) and alkaline phosphatase (AKP), along with the levels of total protein (TP) and albumin (ALB), were significantly increased (<i>P</i> &lt; 0.05). The results indicate that MT pretreatment can safeguard against acute ammonia-N stress, enhancing the antioxidant capacity and innate immunity of the hepatopancreas and hemolymph in the crab. Additionally, ammonia-N stress induced an imbalance in the gut flora and reduced the abundance and diversity of the gut flora. Conversely, the Shannon index of the α-diversity of the gut flora of the Chinese mitten crab was elevated following MT feeding. Therefore, acute ammonia-N exposure inflicted significant oxidative damage and immune function impairment in the hemolymph and hepatopancreas of crabs. However, pretreatment with MT protected the organism and substantially alleviated the damage caused by ammonia-N exposure. Furthermore, these results suggested a close association between the intestine and the hepatopancreas in crabs. Correlation analyses also confirmed that the protective effects of MT might be contingent upon the intestine–hepatopancreas axis.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145316267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Ocimum tenuiflorum L. compounds on Oreochromis mossambicus: a combination of molecular docking, biochemical dynamics, and antifertility assessment","authors":"Sajida Afzal,&nbsp;Siraj Khan,&nbsp;Muhammad Nauman Khan,&nbsp;Zahida Nasreen,&nbsp;Hansa Gul,&nbsp;Mian Inam Zaib,&nbsp;Hameed Alsamadany,&nbsp;Khalid H. Alamer,&nbsp;Ibtisam M. Alsudays,&nbsp;Muhammad Tahir Naseem","doi":"10.1007/s10499-025-02281-5","DOIUrl":"10.1007/s10499-025-02281-5","url":null,"abstract":"<div><p>This study explores the molecular docking and biological impact of <i>Ocimum tenuiflorum</i> L. compounds on <i>Oreochromis mossambicus</i>, particularly investigating antifertility effects. <i>O. tenuiflorum</i> was assessed for its influence on growth, hematological parameters, metabolic enzyme activity, digestibility, and antioxidant enzymes in <i>O. mossambicus</i>. Molecular docking provided insights into interactions between <i>O. tenuiflorum</i> phytochemicals and fertility-related proteins, suggesting potential antifertility effects. Growth performance significantly improved with <i>O. tenuiflorum</i> supplementation, particularly at 30 g/kg (T3). Male fish showed a final body weight increase from 15.10 g (T0) to 22.20 g (T3) and female fish from 14.10 g (T0) to 20.30 g (T3). Percentage weight gain (WG%) and specific growth rate (SGR) also increased significantly, with male WG% rising from 340.00% (T0) to 548.00% (T3) and male SGR from 1.30%/day (T0) to 1.70%/day (T3). Hematological parameters at T3 indicated enhanced health markers, with hemoglobin levels at 11.10 g/dL and packed cell volume (PCV%) at 37.30%. Metabolic enzyme activities remained stable across supplementation levels. However, digestibility coefficients (ADC%) for protein, fat, and dry matter were highest at T3, with ADCp at 84.48%, ADCf at 75.31%, and ADCd at 81.17%. Antioxidant enzyme activities increased significantly with higher supplementation levels. Catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) activities peaked at T3, with CAT at 2.90 µmol/min/mg, POD at 82.29 µmol/min/mg, and SOD at 23.55 µmol/min/mg. Lipid peroxidation (LPO/TBARS) activity decreased, indicating reduced oxidative stress. The study suggests possible antifertility effects through molecular docking, bridging traditional herbal medicine with modern environmental sciences for sustainable aquaculture practices. Further research should focus on in vivo validation and comprehensive ecotoxicological assessments.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145316231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofloc systems as a sustainable strategy to minimize heavy metal bioaccumulation in aquaculture","authors":"Ruhma Ihsan,&nbsp;Aima Iram Batool,&nbsp;Muhammad Fayyaz Ur Rehman,&nbsp;Javaria Ikram,&nbsp;Naima Huma Naveed,&nbsp;Iram Inayat,&nbsp;Syed Sikandar Habib","doi":"10.1007/s10499-025-02294-0","DOIUrl":"10.1007/s10499-025-02294-0","url":null,"abstract":"<div><p>Biofloc systems are emerging as a promising alternative to conventional aquaculture due to their potential to improve water quality, enhance fish health, and minimize contaminant risks. This study investigated the role of biofloc technology in reducing heavy metal bioaccumulation and associated health hazards compared with traditional earthen pond systems. Pangasius fish reared in both systems were analyzed for concentrations of Zn, Fe, Cu, Mn, Ni, Cd, and Pb across liver, kidney, gills, and muscle tissues. Results revealed markedly lower accumulation of all metals in biofloc-reared Pangasius, with reductions ranging from 40–80% compared to earthen ponds. Moreover, water and sediment samples of Biofloc ponds also revealed less metal accumulation as compared to earthen ponds. Muscles were the main site of metal deposition, particularly for Zn and Mn, whereas liver and kidney retained higher levels of Fe and Cd in pond-reared fish. Notably, Pb levels in biofloc fish were nearly six times lower than in pond-reared counterparts, and Cd remained well within international safety limits. Health risk assessments confirmed these trends: biofloc Pangasius, exhibited significantly lower non-carcinogenic risk (HI = 1.09E-01) and carcinogenic risk (TCR = 2.91E-04) compared to earthen pond fish (HI = 3.00E-01; TCR = 6.61E-04). Overall, the findings highlight biofloc technology as a sustainable and health-protective aquaculture strategy, capable of mitigating heavy metal contamination, reducing long-term health risks, and supporting safer fish production systems.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysing the spatiotemporal dynamics of coastal aquaculture along the northern coastal plains of Tamil Nadu, India, using remote sensing time series data","authors":"S. Ravi Prasanth,&nbsp;T. K. Senthamizh,&nbsp;Sulochana Shekhar,&nbsp;L. Gnanappazham,&nbsp;Kumar Arun Prasad","doi":"10.1007/s10499-025-02260-w","DOIUrl":"10.1007/s10499-025-02260-w","url":null,"abstract":"<div><p>Aquaculture is one of the major and fastest emerging food production industries in India, globally ranking second only next to China in terms of total production. In recent decades, with the introduction of <i>Penaeus monodon</i> and <i>Penaeus vannamei</i> shrimp species, the coastal aquaculture in India has shown an exponential growth. The northern coastal plain of Tamil Nadu is one of the major hotspots for the shrimp aquaculture due to the favourable geomorphological and hydrological conditions. In this study, we used Sentinel-1 SAR time series satellite data and the manual extraction approach to map the spatiotemporal distribution of the coastal aquaculture ponds and validated with field visits. With the development of high spatial resolution baseline data of shrimp aquaculture, its distribution, density and hotspots were analysed at micro-administrative level. From the results, it is found that the coastal aquaculture ponds in the study area significantly increased between 2016 and 2024 by 634 ha with an annual change rate of 10.5% with major increase notably in the districts of Thiruvallur (44.4%), Cuddalore (26.4%) and Mayiladuthurai (13.2%). Spatial metrics including total aquaculture pond area (TAPA in %) and aquaculture pond density ratio show that intensity and distribution of aquaculture ponds were dominant in Thiruvallur and Nagapattinam districts. Hotspot analysis shows that the significant clusters of aquaculture concentration are confined near the tidal flats, estuarine zones and backwater networks. Our study contributes to micro-scale aquaculture monitoring by providing baseline information and underscores the importance of micro-level geospatial assessments in guiding sustainable aquaculture development, land use regulation and coastal resource management in vulnerable deltaic regions. This would enable the policymakers and governments in framing integrated coastal resource governance policies considering both environmental factors and human dimensions of aquaculture expansion.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intestinal health and metabolic balance of zebrafish (Danio rerio) fed on diets containing enzymatically hydrolyzed distiller’s dried grains with solubles (DDGS)","authors":"Chun Wang,&nbsp;Xin Peng,&nbsp;Ling Ao,&nbsp;Shan Zeng,&nbsp;Jianping Yang,&nbsp;Caihong Shen,&nbsp;Mengyan Wang,&nbsp;Xiaolong Yao,&nbsp;Yingxue Sun","doi":"10.1007/s10499-025-02288-y","DOIUrl":"10.1007/s10499-025-02288-y","url":null,"abstract":"<div><p>Distiller’s dried grains with solubles (DDGS) were subjected to compound enzymatic deep hydrolysis (CEDEH) and evaluated as a plant-protein substitute for fish meal in zebrafish diets. The CEDEH protocol sequentially applied cellulase and α-amylase followed by proteolysis using neutral and flavor proteases at a 2:1 ratio. Enzyme-treated DDGS were incorporated into experimental diets to partially replace fish meal at 0%, 10%, and 20% inclusion levels. Six dietary treatments were established: DNP10 and DNP20 (neutral protease–hydrolyzed DDGS at 10% and 20%), DFP10 and DFP20 (flavor protease–hydrolyzed DDGS at 10% and 20%), and DCP10 and DCP20 (mixed protease–hydrolyzed DDGS at 10% and 20%). A 30-day feeding trial was conducted on 2-month-old zebrafish to assess physiological responses, intestinal histology, digestive enzyme activity, and gut microbiome–metabolome responses. Notably, CEDEH processing significantly enhanced duodenal villus height (<i>P</i> &lt; 0.01), indicating improved nutrient absorption capacity. At 10% substitution (DCP10 group), hepatic antioxidant capacity significantly improved, with superoxide dismutase (SOD) and glutathione (GSH) activities increasing by 24.8% and 90.8%, respectively, compared to controls (<i>P</i> &lt; 0.05). Gut microbiota restructuring demonstrated functional probiotics enrichment, including <i>Cellulomonas</i> (+ 57.3%) and anti-inflammatory <i>Tabrizicola</i> (+ 354.33%). Concurrently, key digestive enzymes (amylase and trypsin) activities in the duodenum markedly increased by 32.1% and 27.4%. Metagenomic analysis revealed upregulation of key metabolic pathways (ko00020 TCA cycle; ko00290 branched-chain amino acid synthesis). Importantly, 20% substitution (DCP20) preserved intestinal integrity (goblet cells increased by &gt; 40%) without compromising growth performance. These results confirm that CEDEH-treated DDGS is a suitable fish meal substitute at inclusion levels ≤ 20%, promoting host metabolism and disease resistance.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes of secondary sexual characteristics during the growth and development of the male Portunus trituberculatus","authors":"Zhilong Xie,&nbsp;Xiaodong Jiang,&nbsp;Xiaowen Yu,&nbsp;Wenjie Hou,&nbsp;Xugan Wu","doi":"10.1007/s10499-025-02283-3","DOIUrl":"10.1007/s10499-025-02283-3","url":null,"abstract":"<div><p>Allometric growth and secondary sexual characteristics are two important indicators of the morphological maturity and reproduction of crustaceans, but very limited information could be found on the male swimming crab <i>Portunus trituberculatus</i>. Screening based on secondary sexual characteristics thereby provides a scientific basis for selecting sexually mature male crabs in both pond farming and artificial reproduction processes. This study was conducted to investigate the allometric growth of morphological characteristics as well as the changes in secondary sexual characteristics during the period from juveniles to adults of pond-reared male <i>P. trituberculatus</i>. The results showed the following: (1) The univariate variance analysis showed that FFLC, MLC, FPL, and SPL increased significantly between August and September. The principal component analysis showed that FFLC, MLC, FPL, and SPL had higher loading values in the first principal component. (2) According to the allometric growth of ontogeny, five secondary sexual characteristics of male crabs were identified as FFLC, MLC, FPL, SPL, and TPL. The estimates of carapace width for the morphological maturity of males were equal to 91.03 mm, and the maturity of male crabs was concentrated in August. (3) During growth and development, the proportion of blue markings on the cheliped gradually increased, and the <i>b</i> value (yellow–blue value) showed a significant decline. The proportion of blue markings of mature morphology was more than 8.91%, and the yellow–blue value was lower than −5.00. In conclusion, these results showed that FFLC, MLC, FPL, SPL, TPL, and cheliped color were considered six secondary sexual characteristics for the male <i>P. trituberculatus</i> between pre- and post-pubertal molting during the period from August to September, and cheliped color provides a simple, fast, and convenient biomarker to identify morphological maturity.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BDE-47 induces oxidative stress and apoptosis by interfering with pyruvate and glutathione metabolism in Epinephelus coioides","authors":"Zongfu Chen,&nbsp;Lei Yan,&nbsp;Peng Luo,&nbsp;Ziyang Xu,&nbsp;Tongwen Ni,&nbsp;Li Lin,&nbsp;Wei Zhang,&nbsp;Yanan Li","doi":"10.1007/s10499-025-02284-2","DOIUrl":"10.1007/s10499-025-02284-2","url":null,"abstract":"<div><p>2,2',4,4'-Tetrabromodiphenyl ether (BDE-47) is one of the most toxic and widely distributed environmental pollutants, posing a potential threat to <i>Epinephelus coioides</i> aquaculture in China. However, there is currently no information on the toxic effects and mechanisms of BDE-47 on <i>E. coioides</i>. In this study, a 14-day gavage experiment was conducted using BDE-47 on <i>E. coioides</i>, and related indicators were detected by H&amp;E staining, enzyme activity detection, and qPCR. The H&amp;E staining results showed that BDE-47 caused inflammatory infiltration and cell damage in the liver tissue of <i>E. coioides</i>. The enzyme activity detection and qPCR results indicated a decrease in the antioxidant capacity of liver tissue, oxidative damage, and apoptosis. In addition, the in vitro experiments showed that BDE-47 induced an increase in ROS levels and apoptosis. To further explore the toxic mechanism of BDE-47, we conducted transcriptome sequencing analysis. The GO enrichment results suggest that BDE-47 may have an impact on mitochondrial function. The KEGG enrichment results showed that most of the DEGs were enriched in KEGG signaling pathways closely related to mitochondrial function and cellular antioxidant capacity, including pyruvate metabolism, TCA cycle, and glutathione metabolism. These results suggest that BDE-47 inhibit cellular antioxidant capacity by interfering with normal mitochondrial function, resulting in oxidative damage and apoptosis. This study provides a theoretical basis and data support for the control and management of BDE-47 in fishery water environments.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}