Aquaculture International最新文献

{"title":"Importance of biofilms in water quality management in aquaculture systems—a review","authors":"Mamdouh Al- Harbi,&nbsp;Ramzi H. Amran,&nbsp;Sathianeson Satheesh","doi":"10.1007/s10499-025-01953-6","DOIUrl":"10.1007/s10499-025-01953-6","url":null,"abstract":"<div><p>Biofilms, the assemblage of microbial communities on surfaces, play an important role in aquaculture by improving water quality, enhancing the growth and disease resistance of cultured organisms. Microorganisms, especially bacteria associated with biofilms, are involved in the reduction of dissolved solids and pathogens and participate in the nutrient cycling of aquaculture systems. The use of biofilm-based technology in aquaculture is considered an eco-friendly measure for the treatment of wastewater in recirculating aquaculture systems. This review paper explores the role of biofilms in improving water quality in aquaculture systems. While many microorganisms are associated with biofilms, the focus of this paper is on the bacteria community. The mechanisms through which biofilms maintain good water quality and the potential of biofilm-based sustainable aquaculture practices are discussed in detail.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of Myo-inositol on antioxidant capacity and immunity in freshwater crayfish","authors":"Changchang Pu,&nbsp;Yuanyi Liu,&nbsp;Yinfeng Cheng,&nbsp;Bingke Wang,&nbsp;Aimin Wang,&nbsp;Chunnuan Zhang","doi":"10.1007/s10499-025-01949-2","DOIUrl":"10.1007/s10499-025-01949-2","url":null,"abstract":"<div><p>Myo-inositol (MI\u0000) plays a vital role in the growth and development of crustaceans. Although most researchers have focused on the critical role of MI in fish, its application in crustaceans is lacking. A 6-week trial was conducted to comparatively evaluate the effects of 6 levels of dietary MI (0, 500, 1000, 2000, 3000, and 4000 mg/kg diet) in juvenile freshwater crayfish <i>Procambarus clarkii</i> (6.39 ± 0.05) g. This study aimed to investigate the effects of dietary MI on antioxidant capacity, non-specific immunity, and possible molecular mechanisms in freshwater crayfish. Research shows dietary MI could improve hepatopancreatic antioxidant enzyme activity and non-specific immunity (<i>P</i> &lt; 0.05). MI can be converted into phosphatidylinositol 3-kinase (PI3K) under the action of phospholipase, which activates the PI3K/AKT signaling pathway. The PI3K/AKT signaling pathway can promote the transcription level of Nrf2-Keap1, thereby promoting the expression of antioxidant-related genes (<i>sod</i>, <i>cat</i>, and <i>gpx</i>) to improve the antioxidant mechanism (<i>P</i> &lt; 0.05). In addition, dietary MI could prevent the occurrence of endoplasmic reticulum stress (ERS, <i>bip</i>, <i>xbp1</i>, and <i>ire1</i>) and inhibit the expression of inflammatory factors (<i>jnk</i>, <i>il-6</i>, <i>tnf-</i>α, and <i>nf-κb</i>) and apoptosis-related genes (<i>bax</i>, <i>cytc</i>, and <i>casp3</i>) (<i>P</i> &lt; 0.05). Dietary MI improves antioxidant capacity by regulating increased Nrf2 translocation and NF-κB translocation. In conclusion, dietary MI can enhance the antioxidant defense system of freshwater crayfish by activating the PI3K/AKT pathway and coordinating the transcription levels of Nrf2 and NF-κB.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative health assessment of wild and farmed barred spiny eel, Macrognathus pancalus (Hamilton, 1822)","authors":"Farhana Hoque,&nbsp;Subhendu Adhikari,&nbsp;Ajmal Hussan,&nbsp;Baidya Nath Paul,&nbsp;Rathindranath Mandal,&nbsp;Arabinda Das,&nbsp;Subhas Sarkar,&nbsp;Pramoda Kumar Sahoo","doi":"10.1007/s10499-025-01940-x","DOIUrl":"10.1007/s10499-025-01940-x","url":null,"abstract":"<div><p>The barred spiny eel, <i>Macrognathus pancalus</i>, is a high-value food fish with significant market demand but limited aquaculture due to the absence of a defined culture protocol. Health monitoring is crucial for early disease detection, risk assessment, and optimizing rearing conditions and disease management. Hence, this study evaluated the health status of wild <i>M. pancalus</i> from natural waters in West Bengal and farmed counterparts from ICAR-CIFA and RRC, Rahara, to facilitate its integration into aquaculture. The assessment included the study of health indices, hematological profiles, histology, stress biomarkers, and microbial diversity of eel from both environments. Results revealed that wild fish exhibited a higher Health Assessment Index (HAI), greater coefficient of variation (CV), and noticeable organ anomalies, alongside elevated levels of stress biomarkers. In contrast, farmed fish demonstrated significantly (<i>P</i> &lt; 0.05) higher erythro-nuclear dimensions and hemoglobin levels, reflecting better overall health. Parasitological examinations identified the presence of <i>Thelohanellus</i> sp., <i>Trichodina</i> sp., and nematodes in wild fish with marked alterations in liver and kidney tissues. Bacterial loads were higher in wild fish, with <i>Pseudomonadaceae</i> being the dominant bacterial family in both groups. Antibiotic resistance profiling indicated a multiple antibiotic resistance (MAR) index ranging from 0 to 0.58, with higher resistance levels in wild fish. The environmental differences between natural and farmed conditions influenced fish health and microbial diversity. The study highlights the need for environmental management and aquaculture adoption to conserve this high-valued indigenous eel from the wild.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Addition of bromelain enzyme to a plant-based diet in Labeo rohita fingerlings: Effects on growth, body composition, enzyme activities, and intestinal morphology","authors":"Samra Qudratullah,&nbsp;Mahroze Fatima,&nbsp;Syed Zakir Hussain Shah,&nbsp;Noor Khan,&nbsp;Beenish Aftab,&nbsp;Ayesha Khizar,&nbsp;Wazir Ali,&nbsp;Ayesha Tanveer","doi":"10.1007/s10499-025-01946-5","DOIUrl":"10.1007/s10499-025-01946-5","url":null,"abstract":"<div><p>This study investigated the impact of bromelain supplementation in plant based-diet on the growth, enzymatic activity, and intestinal morphology of <i>Labeo rohita</i>. A total of 300 fingerlings (initial weight, 12.27 ± 0.11 g) were acclimatized and randomly distributed into 15 aquaria (20 fish/aquarium), which were fed diets (30% CP) supplemented with bromelain at concentrations of 0, 10, 20, 30, and 40 g/kg (D₀, D₁, D₂, D_3, and D_4, respectively) in triplicate for 90 days at a 3% body weight ratio. Bromelain supplementation up to 20 g/kg significantly (<i>p</i> &lt; <i>0.05</i>) increased weight gain and the specific growth rate; however, higher supplementation levels reduced (<i>p</i> &lt; <i>0.05</i>) growth performance. Similarly, the fish fed up to 20 g/kg bromelain-supplemented diet presented a significantly (<i>p</i> &lt; <i>0.05</i>) lower FCR, and the opposite trend was observed at higher levels. Compared with those in the control group, significant (<i>p</i> &lt; <i>0.05</i>) increases in the activities of digestive enzymes, such as proteases and lipases, were observed in the fish fed diets supplemented with up to 20 g/kg bromelain, and the opposite trend was observed at higher supplementation levels. However, amylase activity was reduced (<i>p</i> &lt; <i>0.05</i>) in the fish fed up to 20 g/kg bromelain supplemented diets in comparison to control group. No significant changes (<i>p</i> &gt; <i>0.05</i>) in survival rate or body composition (moisture, crude fat, ash, or crude protein) were noted. The contents of minerals, particularly copper, zinc, calcium and magnesium, in the fillets decreased significantly (<i>p</i> &lt; <i>0.05</i>) in the fish fed with bromelain-supplemented diets, and the opposite trend was observed for phosphorus. Furthermore, bromelain supplementation up to 20 g/kg significantly (<i>p</i> &lt; <i>0.05)</i> improved serum alkaline phosphatase activity and intestinal morphology, increasing the goblet cell count, mucosal length, villus length, and width; however, higher levels had opposite effects. Moreover, polynomial regression analysis revealed 25.89 g/kg bromelain as the optimal concentration for enhancing the growth and health status of <i>L. rohita,</i> which highlights the potential of bromelain as an effective dietary supplement to increase fish performance in plant-based aquaculture systems. Future research should focus on large-scale applications, economic feasibility, and synergistic effects with other feed additives to reduce the reliance on fishmeal in aquafeed.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A laboratory-scale recirculating aquaculture system for long-term experimental rearing of bivalves under minimal stress conditions for aquatic animal research","authors":"Soumya Balakrishnan,&nbsp;Ambadi Kannan Maliyekkal Sajeevan,&nbsp;Akshay P. S.,&nbsp;I. S. Bright Singh,&nbsp;Jayesh Puthumana","doi":"10.1007/s10499-025-01941-w","DOIUrl":"10.1007/s10499-025-01941-w","url":null,"abstract":"<div><p>Aquatic animal facilities should be designed to minimize stress, ensuring stable environmental conditions that promote animal welfare and enhance experimental reliability. Maintaining bivalves in laboratory tanks with routine water exchange (static-renewal system, SRS) often results in stress and high mortality rates, making long-term rearing challenging. Flow-through systems, while an alternative, require access to seawater and are limited by coastal proximity and regulatory restrictions. Additionally, seasonal collection and transportation of bivalves complicate the consistent supply of animals for carrying out experiments. To address these issues, a simplified recirculating aquaculture system (RAS) was developed to provide stable conditions for bivalves, enabling long-term maintenance with minimal stress for experimental research in aquatic animals. The RAS system integrates culture tanks with solid filtration and biofiltration units to maintain optimal water quality. The RAS was compared to SRS over a three-month rearing period using the bivalve <i>Magallana bilineata</i>. Water quality parameters were assessed every three days, while animal health and mortality were closely monitored. Stress levels were measured by quantitative PCR (qPCR)-based <i>hsp70</i> mRNA expression. The implemented RAS successfully maintained bivalves with minimal stress and stable water chemistry resulting in only 10% mortality in RAS, whereas it was 80% in SRS. The animals in SRS showed significantly elevated <i>hsp70</i> expression (<i>p</i> &lt; 0.05), whereas the expression levels were comparable between those in RAS and the wild (<i>p</i> &gt; 0.05). Therefore, the implemented simple RAS significantly improved the welfare of bivalves, reducing mortality and stress, while supporting long-term maintenance and ensuring the suitability for experimental research in aquatic animals.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated multi-trophic aquaculture (IMTA) of seaweed and shrimp, Litopenaeus vannamei, with partial reduction in feed rate for improved water quality and nutrient efficiency of the culture system","authors":"Huynh Kim Huong,&nbsp;Tran Nguyen Hai Nam,&nbsp;Levis Nambwaya Sirikwa,&nbsp;Le Hoang Vu,&nbsp;Tran Nguyen Duy Khoa,&nbsp;Pham Thi Tuyet Ngan,&nbsp;Nguyen Thi Ngoc Anh","doi":"10.1007/s10499-025-01944-7","DOIUrl":"10.1007/s10499-025-01944-7","url":null,"abstract":"<div><p>Seaweed possesses an incredible ability to absorb excess nutrients (N and P) and serves as a natural food source for marine species, making it a valuable component in integrated aquaculture systems that promote sustainable farming practices. This study investigated the effectiveness of coculturing whiteleg shrimp (<i>Litopeneaus vannamei</i>) with either red seaweed (<i>Gracilaria tenuistipitata</i>) or green seaweed (<i>Cladophora</i> sp.) under partial feed rate decrease was evaluated for 60 days. Five treatments were implemented, each with three replicates: a control group of shrimps in monoculture receiving commercial feed at a 100% feeding rate; and four groups of shrimps cocultured with each seaweed species receiving 100% or 75% of the control treatment’s feed rate. Shrimp juveniles (0.35 ± 0.04 g) were raised at a density of 200 individuals/m³, salinity level of 15 ppt, and seaweed biomass rate of 2 kg/m³. Results indicated that in the polyculture system with a 75% feeding rate, levels of total ammonia nitrogen (TAN), nitrate (NO₃⁻), nitrite (NO₂⁻), and phosphate (PO₄³⁻) as well as counts of heterotrophic bacteria and <i>Vibrio</i> spp. were significantly reduced, indicating that a good culture medium was maintained. Shrimp growth, survival, and yield in polyculture with a 75% feeding rate were significantly higher and the feed conversion ratio was reduced by 36.24–39.28% compared to the control group. Notably, these performance metrics were superior in the <i>G. tenuistipitata</i> group compared to the <i>Cladophora</i> sp. group, suggesting that this red seaweed could be beneficial and more efficient for shrimp polyculture systems. Biochemical analysis of fresh shrimp meat in the coculture revealed that ash was increased and fat was decreased. However, moisture and protein were not significantly impacted compared to the monoculture group.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming waste to value: exploring potential of different organic fertilizer extracts as supplement mineral nutrients in aquaculture wastewater towards sustainable production of Channa striata (Bloch, 1793) and Lactuca sativa L. aquaponics","authors":"Venisza Cathy John,&nbsp;Ajit Kumar Verma,&nbsp;Kishore Kumar Krishnani,&nbsp;Sukham Munilkumar,&nbsp;Tincy Varghese,&nbsp;Chandrakant Mallikarjun Hittinahalli,&nbsp;Akhila S.","doi":"10.1007/s10499-025-01943-8","DOIUrl":"10.1007/s10499-025-01943-8","url":null,"abstract":"<div><p>The expediency of inorganic fertilizer supplementation attributed to the need for synchrony between nutrient requirement of plants and fish is a customary practice, while the utilization of organic fertilizers as a sustainable approach to amend aquaponics production remained largely unexplored. A 90-day trial investigated the feasibility of employing organic fertilizer extract as a supplement to aquaculture wastewater of <i>Channa striata</i>-lettuce aquaponics. Extracts of different organic fertilizers, viz., vermicompost (28.8 mg/L), cow manure (50 mg/L), chicken manure (50 mg/L) and black soldier fly larvae frass (3.3 mg/L) were applied weekly in T1, T2, T3 and T4, respectively and compared to control (C). The water quality varied within tolerable limits for cultured species for most parameters. The supplementation did not induce significant (<i>p</i> &gt; 0.05) changes in the growth performance of fish, while an alteration in normal physiological response was observed in T3 and T4. The antioxidant stress enzymes in the liver exhibited a significant difference in T3, while variation was insignificant in gill. Total lettuce yield (of all three harvests at 30-day interval) followed the order: T1 (1111.88 ± 4.90 g) &gt; T2 (865.87 ± 4.95 g) &gt; T4 (778.63 ± 5.34 g) &gt; T3 (768.67 ± 3.30 g) &gt; C(729.83 ± 7.18 g). Proximate analysis revealed a significant increase in crude protein in T1 (1.59 ± 0.12%), and enrichment of nutrient content of lettuce leaves was perceived in treatments than control. The results indicated that all organic fertilizer extract supplementation performed optimally rendering improved production than control during the study, while vermicompost outperformed significantly in lettuce yield, proximate and mineral composition, and fish physiology, and hence recommended as best feasible for aquaponics.\u0000</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment, characterization, and exploration of the first cell line of Qingtian paddy field carp (Cyprinus carpio var. qingtianensis) and its response to hypoxia mechanism","authors":"Zitong Xiong,&nbsp;Qixuan Li,&nbsp;Yetong Xu,&nbsp;Qigen Liu,&nbsp;Jiamin Sun","doi":"10.1007/s10499-025-01937-6","DOIUrl":"10.1007/s10499-025-01937-6","url":null,"abstract":"<div><p>To explore the stress resistance mechanisms in Qingtian paddy field carp (PF-carp) <i>Cyprinus</i> <i>carpio</i> var. <i>qingtianensis</i> under hypoxic conditions, we established the first fin-derived cell line for this species, termed cells of Qingtian paddy field carp fin (CQF). The CQF cells, primarily fibroblasts, were cultured at 27 °C with 5% CO₂ in the L-15 medium, supplemented with 1% antibiotics and 15% fetal bovine serum. To examine cellular responses to hypoxia, CQF cells were subjected to four conditions: normoxia and hypoxia exposure for 6, 12, and 24 h. With increasing hypoxia duration, abnormal cell morphology, disrupted redox homeostasis, and severe apoptosis were observed. These effects were characterized by elevated reactive oxygen species (ROS) levels, loss of mitochondrial membrane potential (ΔΨm), and upregulation of <i>caspase-3</i>. CQF cells adapted to hypoxia by upregulating antioxidant enzyme activity. Furthermore, hypoxia-inducible factor 1α (HIF-1α) overexpression plasmids were constructed using pcDNA3.1 as a vector, with fluorescent tagging via eGFP-N3 for subcellular localization. Under normoxic conditions, HIF-1α was located in both the cytoplasm and nucleus, but translocated to the nucleus under hypoxia, where it regulated adaptive transcription. Overexpression of HIF-1α in hypoxic CQF cells reduced apoptosis and preserved cell integrity. Thus, the CQF cell line provides a valuable model for investigating autophagy, apoptosis, and gene expression during hypoxia in PF-carp, offering novel insights into hypoxia response mechanisms in fish.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crab species recognition method based on incremental learning","authors":"Duan Qingling,&nbsp;Feng Xiaoxiao,&nbsp;Kong Mingrui,&nbsp;Fu Jiayi,&nbsp;Zhang Ting","doi":"10.1007/s10499-025-01939-4","DOIUrl":"10.1007/s10499-025-01939-4","url":null,"abstract":"<div><p>Crab species are highly diverse, and accurate identification is vital for production management, maturity prediction, yield estimation, automated sorting, and quality grading. Existing deep learning methods typically require all category samples to be input simultaneously, which is impractical because of the challenges of obtaining sufficient training samples and the long-term nature of sample collection. Incremental learning offers a solution by enabling models to learn new categories while retaining knowledge from previous ones. This study proposes a crab species recognition method based on an improved adaptive aggregation networks learning a unified classifier indirectly via rebalancing (AANets-LUCIR) framework. AANets combined with LUCIR serves as the baseline. The ResNet18 backbone is improved by replacing the activation function with ReLU6 to preserve image features and introducing an efficient pyramid split attention mechanism in each BasicBlock module to enhance feature extraction. A <i>k</i>-means clustering-based sample replay strategy is integrated to improve adaptation to new data while retaining old knowledge. Experiments on a self-constructed dataset yielded excellent results, with average scores of 92.30% for adaptability, 89.80% for base, and 91.66% for the final evaluation. Compared with iCaRL, Bic, LwF, EWC, and Replay, the proposed method improved the final scores by 10.15, 5.83, 21.76, 7.2, and 17.61 percentage points, respectively. This approach effectively addresses the incremental crab species classification challenges and supports the development of intelligent aquaculture.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated aquaculture of whiteleg shrimp Litopenaeus vannamei and European seabass Dicentrarchus labrax: impacts on performance, welfare, blood physiological response, carcass traits, productivity, and farm profitability","authors":"Ashraf.I. G. Elhetawy,&nbsp;Abdallah Tageldein Mansour,&nbsp;Ayman M. Lotfy,&nbsp;Ahmed. I. A. Mansour,&nbsp;Shimaa A. Shahin,&nbsp;Mohamed M. Zayed,&nbsp;Ghada R. Sallam,&nbsp;Mohamed M. Abdel-Rahim","doi":"10.1007/s10499-025-01907-y","DOIUrl":"10.1007/s10499-025-01907-y","url":null,"abstract":"<div><p>Seabass farmers suffer from a long-lasting production period of at least 18 months, causing a long-term gap in financial income and payback. This study investigated the integration of <i>Litopenaeus vannamei</i> with <i>Dicentrarchus labrax</i> aquaculture allowing seabass farmers to earn a consistent income 2–3 times a year. Six treatments (seabass alone “control group,” seabass with 5, 10, 15, 20, and 25 shrimp in the fish tanks) were tested in triplicate and expressed as S0, S5, S10, S15, S20, and S25, respectively. Seabass was housed at a standardized density (12 fish/tank, each 3500 L water). Fifteen hapas (each 1 m × 1 m, 700 L water volume) were installed in 15 rearing tanks to hold the experimental shrimp, occupying 20% of the tank’s water volume. Both fish and shrimp received specialized diets during the 90-day experiment. The findings indicated that increasing shrimp density by more than 15 juveniles/hapa significantly (<i>P</i> &lt; 0.05) increased nitrogen by-products (TAN and NH₃) and decreased growth performance and feed efficacy in seabass and shrimp. Moreover, increasing the shrimp density noticeably (<i>P</i> &lt; 0.05) improved the protein content in seabass flesh but declined it in shrimp. Serum and hepatopancreas indicators in seabass and shrimp, encompassing liver and digestive enzymes, lipid profile, cortisol, IgM, antioxidants, and growth immune-related hormones, demonstrated significant (<i>P</i> &lt; 0.05) improvement in groups polycultured with shrimp up to 15 pcs/m² (S15 group). Furthermore, integrating shrimp in seabass tanks improved gross income, net profit, benefit–cost ratio, and payback period of seabass. The recommended shrimp density when integrated with seabass using a flow-through system is 15 pcs/m².</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-025-01907-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}