Aquaculture International最新文献

{"title":"Cell line studies of environmental stress response genes in aquatic model organisms","authors":"Mehtap Bayır,&nbsp;Abdulkadir Bayır,&nbsp;Chunfang Wang,&nbsp;Serpil Turhan,&nbsp;Burcu Naz Uzun,&nbsp;Şeyma Aras","doi":"10.1007/s10499-025-02032-6","DOIUrl":"10.1007/s10499-025-02032-6","url":null,"abstract":"<div><p>Cell line experiments are a convenient and effective means of investigating environmental stress response (ESR) genes using aquatic model organisms. They offer the advantage of eliminating confounding factors that might appear in whole-organism studies (e.g., history of prior exposure, sex, age), allowing for tight control over conditions during an experiment, and reducing the number of animals used, thereby suppressing ethical concerns. Cell lines offer the potential for examination of targeted cellular and molecular processes of stress reactions, e.g., gene expression, protein biosynthesis, and signal transduction pathways. They are reproducible and efficient and thus can be used in high-throughput screening and comparison analysis. The value of cell lines in the assessment of impacts of different environmental stressors on aquatic animals is addressed in this review. We outline the methodology used, key findings relevant to ESR genes and function, and implications of the research on adaptation, identification of biomarkers of stress, conservation, and environmental protection strategy development. This review illustrates ultimately how the studies in cell lines have aided in uncovering the complex dynamics of water animals responding to an evolving environment.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-025-02032-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated evaluation of aquaponic system performance using red tilapia (Oreochromis sp., trihybrid Pargo-UNAM line) and horticultural crops: growth, nutritional quality, and welfare indicators","authors":"Carlos I. Pérez-Rostro,&nbsp;Ruben Paredes-Olguin,&nbsp;Germán Muñoz-Córdova,&nbsp;Martha P. Hernández-Vergara","doi":"10.1007/s10499-025-02053-1","DOIUrl":"10.1007/s10499-025-02053-1","url":null,"abstract":"<div><p>This study evaluated the performance of coupled aquaponic systems, based on a floating raft configuration (deep water culture, DWC), for the cultivation of high-value fruit crops and red tilapia (<i>Oreochromis</i> sp.) under tropical greenhouse conditions. In phase 1, a 2 × 2 factorial design was implemented to assess the effects of two fish stocking densities (114 and 171 fish·m⁻³) and two hydraulic loading rates (6 and 12 m³·m⁻²·day⁻¹) on the production of serrano pepper (Capsicum annuum). In phase 2, the most efficient aquaponic configuration from phase 1 was applied to tomato (<i>Solanum lycopersicum</i>) and cucumber (<i>Cucumis sativus</i>) and compared to hydroponic controls. Water quality remained within optimal ranges across treatments, while plant and fish performance were significantly influenced by stocking density and HLR. The aquaponic treatment with 114 fish·m⁻³ and 12 m³·m⁻²·day⁻¹ produced the best overall results in phase 1. In phase 2, cucumber outperformed tomato in terms of yield, and both aquaponic crops exceeded the productivity of hydroponic controls. Hematological, immunological, and hormonal indicators revealed better physiological status in fish reared under moderate-density aquaponic conditions. The System Efficiency Index (SEI) confirmed that properly balanced aquaponic systems can optimize biological performance and resource use. These findings support the viability of floating raft aquaponics as a sustainable alternative to conventional soilless systems in tropical environments.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterologous expression of a Synechocystis sp. PCC 6803 lipase gene in Synechococcus elongatus PCC 7942","authors":"Xiaotong Ren,&nbsp;Huaiyu Chen,&nbsp;Mengting Wang,&nbsp;Yongzhong Lu,&nbsp;Fang Wang","doi":"10.1007/s10499-025-02052-2","DOIUrl":"10.1007/s10499-025-02052-2","url":null,"abstract":"<div><p>Lipases serve as valuable feed additives in aquaculture by enhancing lipid digestion and nutrient absorption. Recognizing the need for more efficient and sustainable enzyme sources, cyanobacterial lipases for their unique catalytic properties and environmental compatibility were explored. In this study, a lipase gene from <i>Synechocystis</i> sp. PCC 6803 was successfully expressed in <i>Synechococcus elongatus</i> PCC 7942 under the control of the endogenous Pcpc promoter. The recombinant strain showed ~ twofold higher lipase activity (<i>p</i> &lt; 0.01) than wild-type, supported by increased protein expression on SDS-PAGE and enzymatic assays. When used as a feed additive for <i>Synechogobius hasta</i>, the transgenic algal supplement significantly improved growth performance, with a 21.7% increase in final body weight (<i>p</i> &lt; 0.01) and a significantly lower feed conversion ratio (FCR) (<i>p</i> &lt; 0.01) after 30 days of feeding compared to control groups. These findings suggest that the cyanobacterial lipase may serve as a potential feed additive that can enhance both nutritional uptake and growth performance in aquaculture.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and comparison of SYBR green– and TaqMan probe–based quantitative polymerase chain reaction (qPCR) assays for detection of microsporidian parasite Microsporidium seriolae (beko disease) in cultured yellowtail, Seriola quinqueradiata","authors":"Kittipong Thanasaksiri,&nbsp;Ayaka Ikebuchi,&nbsp;Naomi Hanaoka,&nbsp;Shiori Tsubone","doi":"10.1007/s10499-025-02048-y","DOIUrl":"10.1007/s10499-025-02048-y","url":null,"abstract":"<div><p><i>Microsporidium seriolae</i>, an intracellular fungal-related parasite, causes microsporidiosis, also referred to as beko disease, in farmed yellowtail (<i>Seriola quinqueradiata</i>) and amberjack (<i>S. dumerili</i>) in Japan. This disease causes the formation of cysts in the muscles of fry to adult fish. In this study, we described novel SYBR green– and TaqMan probe–based real-time quantitative polymerase chain reaction (qPCR) assays for the detection of <i>M. seriolae</i> based on the newly identified short conserved small subunit ribosomal RNA (SSU rRNA) sequence at the 5′ region. The partial sequences of SSU rRNA of the eight <i>M. seriolae</i> strains were 1814–1820 bp. The developed qPCR primers specifically targeted to the SSU rRNA of <i>M. seriolae</i> but not that of <i>Spraguea</i> sp. in plasmid DNA or clinical sample. The efficiency of SYBR green qPCR assay and TaqMan qPCR assay ranged from 97.1 to 98.0% and 97.2 to 98.4%, respectively, and the sensitivity of both assays using diluted plasmid was 0.5 × 10⁴ copies/20 μl reaction. Both assays could detect <i>M. seriolae</i> at 0.4 ng of total genomic DNA from a <i>M. seriolae</i>-infected yellowtail. No difference in qPCR amplification efficiency was observed between the two methods when clinical samples were used. These results suggest that the developed qPCR assay is a useful tool for the rapid detection of <i>M. seriolae</i> and can facilitate epidemiological investigations in a yellowtail aquaculture.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing technical efficiency in whiteleg shrimp aquaculture: a double-bootstrap data envelopment analysis","authors":"Kalidoss Radhakrishnan,&nbsp;Baban Bayan,&nbsp;Swadesh Prakash,&nbsp;P. S. Ananthan,&nbsp;Anu Susan Sam,&nbsp;Sandip Shil,&nbsp;Arpita Sharma,&nbsp;Srichandan Das,&nbsp;Kota Karuna Sri","doi":"10.1007/s10499-025-02036-2","DOIUrl":"10.1007/s10499-025-02036-2","url":null,"abstract":"<div><p>Maximizing shrimp yield necessitates the efficient utilization of key inputs to enhance technical efficiency and overall production performance. This study employs Data Envelopment Analysis (DEA) with a double-bootstrap procedure to derive statistically robust efficiency estimates. Using cross-section data of 256 semi-intensive whiteleg shrimp (<i>Litopenaeus vannamei</i>) farms in Tamil Nadu, India, the study shows that most farms operate below the production frontier with an average technical efficiency score of bias-corrected, radial, and non-radial measures equivalent to 0.57, 0.60, and 0.38, respectively. Notable inefficiencies in input utilization underscore the need for targeted interventions to refine resource allocation strategies. The divergence between radial and non-radial efficiency scores suggests that while overall input use is relatively efficient, further optimization in specific resource management practices is required. The determinants of technical efficiency scores are farm size, stocking density, culture duration, and specific growth rate, all exerting statistically significant positive effects on shrimp yield. Findings highlight the critical role of strategic resource allocation to enhance farm performance by improving productivity, which helps ensure the long-term viability of shrimp aquaculture.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review on the development and efficacy of nano-based vaccine in aquaculture","authors":"Shamsu Ibrahim Ishaq,&nbsp;Muhammad Salahudin Kheirel Anuar,&nbsp;Abdulwakil Olawale Saba,&nbsp;Ina Salwany Md Yasin,&nbsp;Che Azurahanim Che Abdullah,&nbsp;Annas Salleh,&nbsp;Muhammad Farhan Nazarudin,&nbsp;Mohd Termizi Yusof,&nbsp;Mohammad Noor Amal Azmai","doi":"10.1007/s10499-025-02026-4","DOIUrl":"10.1007/s10499-025-02026-4","url":null,"abstract":"<div><p>Nano-based vaccine technology has become one of the solutions in aquaculture disease management as it provides enhanced immunity to the host organisms and reduces the use of antibiotics. This systematic review evaluates the nano-based vaccine development, formulations, and efficacy in improving animal survival rates, immune responses, and disease resistance, while identifying challenges and future directions of the nano-based vaccines technology in aquaculture. The reviews found that different nano-based vaccine formulations, such as chitosan nanoparticles, mesoporous silica nanocarriers, and biomimetic systems for DNA delivery, are proven to show high protective efficacy against pathogens such as <i>Streptococcus agalactiae</i> and <i>Aeromonas hydrophila</i>. Studies report survival rates between 83.3 and 96.2%, significant upregulation of immune markers and enhanced lysozyme activity. Nano-based vaccines also exhibit potent antibacterial activity, particularly in shrimp, with dsRNA-loaded nanoparticle vaccine improving survival by up to 80% against white spot syndrome virus. Although these developments are evident, the differences in the effect of the vaccine applications result from variations due to formulation strategies and host organisms of interest and variability in the environmental conditions remains a more significant hindrance. This review provides information regarding the potential applications of nano-based vaccine technology for the advancement of aquaculture that ensures disease control through the reduced use of antibiotics and sustainable global food security.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drivers of carbon footprint variability in shrimp aquaculture: insights from aquamimicry shrimp farming","authors":"Ratthanin Sangsayan,&nbsp;Manoj Tukaram Kamble,&nbsp;Krishna Rugmini Salin","doi":"10.1007/s10499-025-02040-6","DOIUrl":"10.1007/s10499-025-02040-6","url":null,"abstract":"<div><p>Aquaculture continues to expand globally, particularly in Asia, with production reaching 130.9 million tons, valued at US$ 312.8 billion in 2022. The adoption of sustainable practices, such as Aquamimicry Shrimp Farming (ASF), is crucial for mitigating environmental impacts while enhancing food security. Aquamimicry applies biomimicry principles to replicate natural aquatic ecosystems within closed farming environments, thereby promoting effective waste management and optimizing shrimp production. This study assessed the carbon footprint (CF) of ASF operations in Prachuap Khiri Khan Province, Thailand, using a Life Cycle Assessment (LCA) framework combined with statistical techniques, including descriptive statistics, multivariate analysis, and stepwise regression, to evaluate different production levels. Data were collected from 40 shrimp farms practicing ASF under Good Agricultural Practices (GAP) certification scheme. Farms were classified into three groups based on yield: Group H (high production, over 10 tons/ha), Group M (medium production, 5–10 tons/ha), and Group L (low production, ≤ 5 tons/ha), to investigate the association between farming practices and carbon emissions. Results indicated that improved management practices were positively associated with increased productivity and lower carbon footprints, with Group H exhibiting the most favourable sustainability indicators. Electricity consumption emerged as the primary contributor to total carbon emissions (51.35%), while the rearing phase accounted for the majority of emissions (88.05%). A negative correlation was observed between carbon footprint and shrimp survival rates (r = -0.357), underscoring the importance of efficient farming strategies. Stepwise regression identified average harvest size and stocking density as significant factors influencing production outcomes. Well-managed farms in Group H achieved better feed conversion ratios, higher survival rates, and lower emissions per unit of shrimp produced. In conclusion, the findings highlight that effective management within Aquamimicry systems enhances both production efficiency and environmental sustainability, reinforcing the potential of ASF as a promising strategy for sustainable shrimp farming.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on the influencing factors and configuration path of environmental technological progress bias in China’s mariculture industry","authors":"Jianyue Ji,&nbsp;Yanan Sun,&nbsp;Longyu Xu,&nbsp;Yao Xu","doi":"10.1007/s10499-025-02038-0","DOIUrl":"10.1007/s10499-025-02038-0","url":null,"abstract":"<div><p>This paper explores how to drive the environmental technological progress bias of mariculture by using the data of China’s coastal areas from 2008 to 2021. The panel regression model and dynamic qualitative comparative analysis (QCA) method are applied to empirical research. The regression results of influencing factors show that environmental regulation, technological innovation, fisherman’s income, promotion facilities, and opening up have a significant impact on the environmental technological progress bias. The results of configuration path show that there are three configuration paths to drive the environmental technological progress bias, namely, economy-driven path, technology–promotion-driven path, and economy–technology–promotion-driven path. The temporal changes of the three configurations are stable, and the explanatory power is gradually enhanced. The study could explore the multi-factor mechanism behind the environmental technological progress bias and provide guidance for driving the environmental technological progress bias of mariculture industry.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fish meal substitution by meat and bone meal in large yellow croaker Larimichthys crocea diet: integrated insights on growth performance, intestinal morphology and microflora, antioxidant capacity, and protein metabolism","authors":"Dianguang Zhang,&nbsp;Zhangqi Li,&nbsp;Qianqian Liu,&nbsp;Zhengbang Chen,&nbsp;Jianchun Shao,&nbsp;Xinhua Chen","doi":"10.1007/s10499-025-02018-4","DOIUrl":"10.1007/s10499-025-02018-4","url":null,"abstract":"<div><p>The present feeding study was carried out on <i>Larimichthys crocea</i>, with an initial weight of 18.36 ± 1.72 g, to assess the effects of substituting fish meal (FM) with meat and bone meal (MBM) on various factors, such as growth, intestinal morphology and microbiota, protein metabolism, and antioxidant ability. The five diets were created with varying percentages of FM replaced by MBM: 0% (MBM0), 25% (MBM25), 50% (MBM50), 75% (MBM75), and completely without FM (MBM100). The results exhibited that replacing 50% of dietary FM with MBM had no significant impact on fish growth or feed efficiency. An examination of intestinal morphology indicated notable reductions in villus width (VW), villus height (VH), and muscle thickness (MT) in the MBM75 and MBM100 groups. The MBM75 and MBM100 diets significantly reduced the activity levels of amylase (AMS), lipase (LPS), catalase (CAT), total superoxide dismutase (T-SOD), and total antioxidant capacity (T-AOC), while concurrently elevating the malondialdehyde (MDA) content considerably. 16S rRNA sequencing indicated that the MBM75 and MBM100 diets led to an elevation in the proportion of <i>Proteobacteria</i> and a reduction in the proportion of <i>Firmicutes</i>. Furthermore, immunoblotting analysis showed that in the muscle tissue, the MBM75 and MBM100 diets caused a notable reduction in the protein levels of 4E-BP1 (Thr37/46) and mTOR (Ser2448). Broken-line analysis revealed that the optimal substitution levels of meat and bone meal for fish meal were 40.60%, 50.00%, 42.08%, and 50.00% based on weight gain (WG), specific growth rate (SGR), feed conversion rate (FCR), and protein efficiency ratio (PER), respectively.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterosis analysis of heat and cold tolerance in hybrid offspring of sea urchin Mesocentrotus nudus: Implications for breeding","authors":"Wenzhuo Tian,&nbsp;Xuechun Jiang,&nbsp;Hao Guo,&nbsp;Peng Liu,&nbsp;Tongshan Jia,&nbsp;Shuaichen Wu,&nbsp;Fanjiang Ou,&nbsp;Xiaofei Leng,&nbsp;Xuguang Li,&nbsp;Lijie Wang,&nbsp;Yaqing Chang,&nbsp;Weijie Zhang,&nbsp;Jun Ding","doi":"10.1007/s10499-025-02044-2","DOIUrl":"10.1007/s10499-025-02044-2","url":null,"abstract":"<div><p>This study investigated the heterosis of heat and cold tolerance in hybrid offspring derived from Dalian, Liaoning, China (DL) and Weihai, Shandong, China (WH) populations of the sea urchin <i>Mesocentrotus nudu</i>s. Through chronic heating and cooling experiments, we compared four experimental groups: reciprocal hybrids (WH ♀ × DL ♂ [WD], DL ♀ × WH ♂ [DW]) and parental controls (WH ♀ × WH ♂ [WW], DL ♀ × DL ♂ [DD]). Survival time, upper thermal tolerance (UTT), and cooling-degree days (CDD) were analyzed with heterosis calculations. Under chronic heating stress, the WD hybrid exhibited: 1. Significantly prolonged survival time compared to WW and DW groups (Log-rank test, <i>P</i> &lt; 0.05). 2. Enhanced UTT with 5.0% mid-parent heterosis (MPH) and 5.9%/4.2% single-parent heterosis (SPH) over WW/DL respectively. The heat tolerance of the four populations ranked as: WD &gt; DD ≥ WW ≥ DW. During chronic cold exposure, WD demonstrated: 1. Superior survival duration and CDD relative to all groups (<i>P</i> &lt; 0.05). 2. 11.5% MPH with 12.9%/10.0% SPH over WW/DL. The cold tolerance of the four populations ranked as: WD &gt; DW ≥ DD ≥ WW. Notably, DW hybrids displayed negative heat heterosis (-2.3% MPH) but marginal cold heterosis (3.8% MPH). These findings identify WD hybrids as promising germplasm for breeding temperature-resilient sea urchin strains, with specific heterosis patterns suggesting maternal effects in thermal adaptation.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}