Aquaculture International最新文献

{"title":"Fish oil replacement with linseed oil affects growth, tissue fatty acid composition, blood chemistry, and antioxidant biomarkers in rohu, Labeo rohita","authors":"Muhammad Asghar,&nbsp;Noor Khan,&nbsp;Mahroze Fatima,&nbsp;Seyda Tacer-Tanas,&nbsp;Simon John Davies,&nbsp;Naveed ul Haque,&nbsp;Ayesha Tanveer,&nbsp;Murat Arslan","doi":"10.1007/s10499-025-01888-y","DOIUrl":"10.1007/s10499-025-01888-y","url":null,"abstract":"<div><p>The effect of replacing fish oil (FO) with linseed oil (LnO) in rohu, <i>Labeo rohita</i> (initial weight 19.1 ± 0.1 g), diets was evaluated in a 90-day feeding trial. Five isonitrogenous (30% crude protein) and isolipidic (11% crude lipid) diets were prepared by substitution of FO (7% FO in the control) with LnO (0, 25, 50, 75, and 100%). Responses of the fish were tested in triplicate for each diet. Partial replacements up to 75% did not cause any significant change in growth performance and feed efficiency. However, total replacement of FO significantly reduced growth performance and feed utilization (<i>P</i> &lt; 0.05). Survival, hepatosomatic index (HSI), and viscerosomatic index (VSI) as well as whole-body proximate composition were not significantly influenced by the dietary treatments. The ratio of alpha linolenic acid (ALA; 18:3n-3) increased in the muscle and liver with the increasing level of dietary LnO, while the percentage levels of eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) decreased. However, EPA and DHA were in considerable level (3.3 and 18.6%, respectively) in muscle even when fed diet with 100% LnO. Hematological parameters such as red blood cell (RBC), hemoglobin (Hb), and hematocrit (Hct) were not influenced by dietary treatments, averaging 2.2 × 10⁶ U/L, 7.6 g/L, and 27.8%, respectively. On the other hand, alanine transaminase (ALT) and aspartate transaminase (AST) activity in serum were significantly elevated with the LnO inclusion levels (<i>P</i> &lt; 0.05), reaching the highest levels (33.0 and 310.7 U/L, respectively) when LnO totally replaced FO. Cholesterol (CHOL), triacylglycerol (TG), high-density lipid (HDL), and low-density lipid (LDL) levels were significantly enhanced (<i>P</i> &lt; 0.05) compared to the control. Cortisol (CORT) and glucose (GLU) were not significantly changed, averaging 1.5 nmol/mL and 20.8 g/dL, respectively. In the liver and serum, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities were significantly reduced, while malondialdehyde (MDA) level was significantly increased (<i>P</i> &lt; 0.05) with the dietary LnO inclusion. Our results indicate that FO can be substituted with LnO up to 75% in practical diet formulations for rohu without any considerable negative effect, which will substantially decrease the dependency on dietary FO.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143521594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variations in mucous cell parameters in the gills and skin of farmed Atlantic salmon (Salmo salar) from freshwater flow-through and recirculating aquaculture systems compared to the gills of wild Atlantic salmon juveniles","authors":"Grigory V. Merkin,&nbsp;Albert Girons,&nbsp;Karin Pittman","doi":"10.1007/s10499-025-01894-0","DOIUrl":"10.1007/s10499-025-01894-0","url":null,"abstract":"<div><p>This study describes mucous cell (MC) parameters in barrier epithelial tissue of Atlantic salmon (<i>Salmo salar</i>) juveniles reared in freshwater flow-through (FT) and recirculating aquaculture systems (RAS). Using Veribarr™ technology, we found no significant differences in the mean MC size and abundance in the gill lamella and skin between FT and RAS systems (<i>p</i> &gt; 0.05, lme). Notably, 100% of gill lamella MC parameters in RAS facilities fell within the most common range (mean ± 0.5 standard deviation, 38% confidence interval) for farmed salmon gill mucosa, whereas MC parameters in only 20% of FT facilities fell in this most typical range. The differences between individual FT facilities resulted in a greater variability in MC parameters compared to RAS facilities, especially in the gills, likely due to a more controlled environment in RAS. MC sizes and abundances in gills at some FT facilities were comparable to those reported in wild Atlantic salmon juveniles. This finding suggests that the water conditions at these facilities may not evoke a response from the gill mucosa, possibly indicating an environment better suited to the natural physiology of Atlantic salmon. Facility-specific conditions, rather than system-specific conditions, play an important role in the health of salmon barrier tissues. The study underscores the potential of using MC parameters as a monitoring tool, paving the way for innovative baseline health promotion as a preventative disease control strategy in aquaculture systems.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143521595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal dynamics and phylogenetic analysis of Eustrongylides: A zoonotic parasite infesting spotted snakehead (Channa punctata)","authors":"Julfat Tasnim Suchona,&nbsp;Siyam Hossain,&nbsp;Anita Rani Dey,&nbsp;Tanvir Rahman,&nbsp;Salman Shahriar Nibir,&nbsp;A.G.M.Sofi Uddin Mahamud,&nbsp;Jarin Tasnim Tanwi,&nbsp; Ashfaq-Un-Nahar,&nbsp;Savayan Sadad Bushra,&nbsp;Khadiza Tul Kubra,&nbsp;Tamanna Tabassum,&nbsp;Shadman Shakib Shovon,&nbsp;Istiak Ahmed","doi":"10.1007/s10499-025-01895-z","DOIUrl":"10.1007/s10499-025-01895-z","url":null,"abstract":"<div><p><i>Eustrongylides</i> are a nematode parasite of fish and fish-eating birds and have a potential health hazard in humans. The present study was designed to determine seasonal dynamics and molecular characterization of <i>Eustrongylides</i> spp. in <i>Channa punctata</i> collected from Trishal, Mymensingh. A total of 385 fish samples were collected and processed, and isolated parasites were examined under a microscope. After morphological examination, twenty (20) parasites were considered for sequencing using the <i>internal transcribed spacer 2</i> (<i>ITS-2)</i> gene. The isolated parasites were identified as fourth-stage larvae (L₄) of <i>Eustrongylides</i> according to their morphological features. Of 385 fish samples, 79 were infected with <i>Eustrongylides</i> spp., and overall prevalence, mean intensity, and abundance were 20.5%, 1.86 ± 0.18, and 0.38, respectively. The highest prevalence was found in the rainy season (27.8%), followed by autumn (24.3), and the lowest in winter (15.6) and summer (14.6%). Medium-sized fish (24.5%) were more prone to parasitic infestation than smaller (16.6%) and larger (14.5%) ones. Parasites were more abundant in body cavity (79.5%) than muscle (20.5%). Among 20 sequences, one genotype was identified and showed 100% similarities with reference sequences of <i>Eustrongylides</i> spp. The unique clustering pattern confirmed morphological identification as <i>Eustrongylides</i> spp. Detailed molecular study is essential to explore the genetic diversity pattern of <i>Eustrongylides</i> in <i>C. punctata</i>.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of photoperiod and temperature on sexual maturation in Masu salmon (Oncorhynchus masou)","authors":"Satoshi Imai,&nbsp;Daisuke Ojima,&nbsp;Kei Yuasa,&nbsp;Daisuke Shimizu","doi":"10.1007/s10499-025-01898-w","DOIUrl":"10.1007/s10499-025-01898-w","url":null,"abstract":"<div><p>Improving smolt production in Masu salmon is pertinent for controlling its sexual maturation. Both photoperiod and temperature considerably influence salmonid sexual maturation. The first experiment began on June 8 in 2021, with four groups: (1) Advanced Photoperiod (AP; natural photoperiod shifted to 18L, then reduced to 8L after 30 days), (2) temperature manipulation (Advanced Temperature (AT): ambient temperature (10 ˚C) raised to 18 ˚C at 0.5 ˚C/day, then reduced to ambient (12 ˚C) at 1 ˚C/week after 21 days), (3) combined AP + AT, and (4) a control group under natural photoperiod and ambient temperature (no environmental manipulation). In the AP group, plasma steroid hormone levels increased earlier than those in the control group, with ovulation (Sep. 3–22) and exuded milt (Aug. 26–Sep. 3) occurring sooner. AT alone did not affect ovulation timing (Sep. 7–Oct. 18) or exuded milt (Aug. 26–Sep. 27). However, AP + AT manipulation resulted in a smooth transition from elevated estradiol-17β in females and 11-ketotestosterone in males to ovulation (Aug. 20–Sep. 17) and exuded milt (Aug. 6–20). Ovulation and exuded milt were latest in the control group. The second experiment, which began on June 13 in 2022, confirmed the reproducibility of early ovulation and exuded milt in the AP + AT group. These findings demonstrate, for the first time, that sexual maturity in anadromous Masu salmon can be controlled by manipulating photoperiod and water temperature. This approach is expected to be a viable means of extending the growth period for 0 + spring smolts.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143521612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic expression of retinol dehydrogenase 12 and all-trans-retinol 13,14-reductase during acute hepatopancreatic necrosis disease in Penaeus monodon: insight into retinoids metabolism in pathogenesis","authors":"Hassan Alaidarous,&nbsp;Subha Bhassu,&nbsp;Samudi Chandramathi,&nbsp;Ismail Yusoff,&nbsp;Tze Chiew Christie Soo","doi":"10.1007/s10499-025-01878-0","DOIUrl":"10.1007/s10499-025-01878-0","url":null,"abstract":"<p><i>Vibrio parahaemolyticus</i> (VpAHPND), the bacterium that causes acute hepatopancreatic necrosis disease (AHPND), is a severe hazard to shrimp aquaculture worldwide. Despite substantial research into AHPND pathophysiology, the role of retinoid metabolism in shrimp immunological responses remains unknown. We aimed to investigate the functions of retinol dehydrogenase 12 (RDH12) and all-trans-retinol 13,14-reductase (AT-R 13,14-R) during VpAHPND infection to better understand their contribution to retinoid metabolism and immunological responses in <i>Penaeus monodon</i>. Toward this, hepatopancreatic tissue samples were obtained at different time points (0, 3, 6, 12, 24, 36, and 48 h post-infection (hpi)) from <i>P. monodon</i> infected with VpAHPND (KS17.S5-1 strain). Quantitative real-time PCR (qRT-PCR) analysis of these samples revealed a substantial overexpression of RDH12 (sixfold) in the infected group compared to the control, with a peak expression of 6.11 ± 0.15-fold at 12 hpi. AT-R 13,14-R showed a ninefold downregulation, with the lowest expression of − 9.69 ± 0.04-fold at 3 hpi. These findings suggest that AHPND affects the retinoid signaling system, jeopardizing immune defenses and metabolic control. Thus, restoring this system may reduce the impact of harmful chemicals, leading to new treatment options, such as dietary supplementation with retinoid precursors, to protect shrimp farms against AHPND.</p>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing light: innovative photodynamic therapy for controlling Aeromonas hydrophila in aquaculture","authors":"Gelana Urgesa Ayana,&nbsp;Mukarim Abdurahaman Kadir,&nbsp;Sahr Lamina Sumana,&nbsp;Geleta Tiko Welde,&nbsp;Cosmas John Kaiyira","doi":"10.1007/s10499-025-01889-x","DOIUrl":"10.1007/s10499-025-01889-x","url":null,"abstract":"<div><p>Aquaculture is a rapidly growing sector that plays a crucial role in meeting global food demands and providing livelihoods. However, industry faces significant challenges from pathogens like <i>Aeromonas hydrophila</i>, which can lead to severe economic losses. Traditional treatments, such as antibiotics, have become less effective due to the rise of antibiotic resistance, necessitating the exploration of alternative strategies. This review explores the potential of photodynamic therapy (PDT) as an innovative approach to controlling <i>A. hydrophila</i> in aquaculture and evaluates its implications for sustainable practices. We conducted a comprehensive review of the literature on PDT, focusing on its mechanisms, the role of photosensitizers, nanotechnology, pathogen control, photocatalysis, and the generation of reactive oxygen species (ROS). We also examined the logistical challenges of implementing PDT in aquaculture and the need for optimized treatment protocols. Our findings indicate that PDT presents a promising alternative for pathogen control in aquaculture. It also highlights the potential applications of natural and synthetic photosensitizers, emphasizing their role in sustainable aquaculture practices. We also discussed the current challenges in implementation of PDT in aquaculture, logistical issues, light sources, and treatment timing. Integrating methodologies such as PSs and nanotechnology could enhance the efficacy of PDT in combating <i>A. hydrophila</i> and other pathogens. By addressing current challenges and optimizing protocols, PDT has the potential to contribute significantly to sustainable aquaculture, improving food security, and reducing reliance on antibiotics.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of velvet bean (Mucuna pruriens) seed extract (VBE) supplementation on reproductive hormones, growth, and gonad development in male and female Jatimbulan tilapia (Oreochromis sp.)","authors":"Ahmad Shofy Mubarak,&nbsp;Kamiliya Zahrah Taher,&nbsp;Putri Eriza Riyanto,&nbsp;Raditya Nur Bahij Baihaqi,&nbsp;Akhmad Taufiq Mukti,&nbsp;Syifania Hanifa Samara,&nbsp;Dita Wisudyawati,&nbsp;Yudi Cahyoko,&nbsp;Muhamad Amin","doi":"10.1007/s10499-025-01890-4","DOIUrl":"10.1007/s10499-025-01890-4","url":null,"abstract":"<div><p>This study investigated the effects of velvet bean (<i>Mucuna pruriens</i>) seed extract (VBE) supplementation on growth performances, gonadomoatic index (GSI), dopamine levels, follicle-stimulating hormone (FSH) levels, and feed digestibility in male and female Jatimbulan tilapia (<i>Oreochromis</i> sp.). A completely randomized design was used, with five doses of velvet bean extract (VBE) (0, 2, 3, 5, and 7 mL/kg) and four replicates. The solution (70 mL) of VBE, carboxymethyl cellulose (CMC), and distilled water was mixed evenly into a 2-mm commercial feed pellet (Hi-Provite 781–2), which contained 33% crude protein and 4% crude fat. Jatimbulan tilapia (5–7 cm in length and 2.70–3.21 g of weight) were sourced from the Umbulan Aquaculture Installation in Pasuruan, East Java, Indonesia. A total of 1000 tilapia (500 males and 500 females) were randomly assigned to 20 floating hapa nets (2 × 1 × 1 m) according to the treatments at a stocking density of 25 fish/m³ and cultured for 60 days and fed the assigned treatments. Growth parameters were measured every 10 days, while gonadal development and hormone levels were assessed at the end of the culture period. The results showed that VBE supplementation significantly increased dopamine levels and decreased FSH levels in both male and female tilapia (<i>p</i> &lt; 0.05). The highest dopamine level (950 pg/mL) was observed in fish fed with 5 mL/kg of VBE, while the lowest FSH level (1.34 ng/mL) was recorded in fish consuming feed supplemented with 7 mL/kg of VBE. Supplementation of VBE significantly affected the gonad somatic index (GSI) of Jatimbulan tilapia (<i>p</i> &lt; 0.05). GSI and gonadal maturity levels in females were reduced with the increasing VBE supplementation (the lowest GSI with 7 mL/kg diet), whereas GSI and gonadal maturity in males tilapia were enhanced with the increasing supplementation concentration (the highest GSI T 7 mL/kg diet). Additionally, VBE improved specific growth rate and feed digestibility in both tilapia sexes, with the best results observed at 5 mL/kg supplementation. These findings suggest that VBE may modulate reproductive hormone levels while promoting growth performance in Jatimbulan tilapia, offering potential applications in aquaculture management. This study can be necessarily continued to investigate the efficacy of velvet bean seed extract on other species.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the probiotic effects of Lactobacillus sakei 2–3 and Candida zeylanoides Y12-3 strains in rainbow trout (Oncorhynchus mykiss, Walbaum 1792)","authors":"Mohanad Dheyaa Taha,&nbsp;Behire Işıl Didinen,&nbsp;Sevdan Yilmaz,&nbsp;Mert Gürkan,&nbsp;Osman Nezih Kenanoğlu","doi":"10.1007/s10499-025-01885-1","DOIUrl":"10.1007/s10499-025-01885-1","url":null,"abstract":"<div><p>This study aimed to assess individual and combined effects of candidate probiotic strains <i>C. zeylanoides</i> Y12-3 and <i>L. sakei</i> 2–3 on growth, hematological parameters, serum immunological parameters, serum biochemistry, histopathology, histomorphology, expression of immune and antioxidant enzyme genes, and disease resistance against <i>L. garvieae</i> in rainbow trout. The fish were fed four different feeds (control, <i>L. sakei</i> 2–3, <i>C. zeylanoides</i> Y12-3, and <i>L. sakei</i> 2–3 + <i>C. zeylanoides</i> Y12-3) for 60 days. At the end of the experiment, growth parameters, serum glucose levels, serum lysozyme activity, and expression of immune and antioxidant enzyme genes were significantly increased in the probiotic groups. Additionally, triglyceride levels decreased in the probiotic groups compared to the control group, whereas serum ALT levels did not change. The villus width and the number of goblet cells increased in the proximal intestines of the fish in <i>C. zeylanoides</i> and <i>L. sakei</i> + <i>C. zeylanoides</i> groups. <i>L. sakei</i> 2–3 showed higher superoxide anion production, expression of immune genes (IgM, IL-B1, lysozyme, TNF-α, HSP70) in the kidney, antioxidant enzyme genes (GPX, GST, SOD) in the liver compared to <i>C. zeylanoides</i>. TNF-α, HSP70), and antioxidant enzyme genes (GPX, GST, SOD) compared to <i>C. zeylanoides</i>. <i>L. sakei</i> and the combination of <i>L. sakei</i> + <i>C. zeylanoides</i> provided resistance to <i>L. garvieae</i> compared to the control group. However, <i>C. zeylanoides</i> was similar to the other two probiotic groups regarding disease resistance against <i>L. garvieae</i>. However, histopathological examinations revealed reversible changes in the proximal intestine, anterior kidney, and liver of fish in the <i>C. zeylanoides</i> and <i>L. sakei</i> + <i>C. zeylanoides</i> groups. Hence, future studies are still required to explore the effects of shorter-term use of the <i>C. zeylanoides</i> strain in rainbow trout to prevent undesirable effects on tissues. In brief, the findings, as mentioned above, showed that <i>L. sakei</i> 2–3 and <i>C. zeylanoides</i> Y12-3 could be potential probiotic candidates for use in rainbow trout farming. Moreover, the probiotic effects of both strains on different fish species should also be studied.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-025-01885-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-time detection of hypoxic stress behavior in aquaculture fish using an enhanced YOLOv8 model","authors":"Chengqing Cai,&nbsp;Shuangyi Tan,&nbsp;Xinmiao Wang,&nbsp;Bohao Zhang,&nbsp;Chaowei Fang,&nbsp;Guanbin Li,&nbsp;Longqin Xu,&nbsp;Shuangyin Liu,&nbsp;Ruixin Wang","doi":"10.1007/s10499-025-01886-0","DOIUrl":"10.1007/s10499-025-01886-0","url":null,"abstract":"<div><p>Prolonged hypoxic conditions pose a significant threat to the survival of fish in aquaculture, often leading to mass mortality events. Abnormal fish behavior, particularly under hypoxic stress, can be an early warning indicator of decreasing dissolved oxygen levels in water. However, existing methods for detecting hypoxic stress behavior in fish are affected by the lighting, occlusion, and turbidity in real aquaculture environments. This results in low accuracy in detecting hypoxic stress behaviors. In this paper, we propose a real-time detection method, YOLOv8n-HSB, designed to enhance the accuracy of detecting hypoxic stress behavior in tilapia within recirculating aquaculture systems. Key improvements of our approach include (1) the introduction of the Multi-scale Fusion Pyramid Network (MFP-Net), which enhances small object detection by adding a specific layer at the bottom of the feature pyramid and improving feature fusion based on Bi-directional Feature Pyramid Network (BIfpn) architecture for the neck structure; (2) the development of the C2f-Occlusion Perception (C2f-OP) module in the backbone by integrating Mobile Inverted Residual Bottleneck Convolution (MBConv) and Effective Squeeze-and-Excitation (ESE), improving the model’s ability to capture crucial local features; and (3) the replacement of conventional Convolution (Conv) layers with Dynamic Convolution (DConv) modules integrated with ParameterNet (P-DConv), enhancing the model’s capacity to process complex information and extract fine-scale features of fish. Experimental results demonstrate that the YOLOv8n-HSB model is highly effective for detecting hypoxic stress behavior in tilapia. Compared to the original YOLOv8n model, the AP_@0.5:0.95 increases by 4.05%. The AP_@0.5 reaches 96.12%, outperforming existing state-of-the-art methods. This study provides a novel method for monitoring the abnormal behavior of fish in hypoxic environments, offering practical significance for smart aquaculture systems.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Development of a lateral flow immunochromatographic assay for the detection of Lactococcus garvieae in grey mullet (Mugil cephalus)","authors":"Choon-Sup Kim,&nbsp;Sung-Hun Lee,&nbsp;Myoung Sug Kim,&nbsp;Kyoung-Hui Kong,&nbsp;Hwi-Jin Kim,&nbsp;Gee-Wook Shin,&nbsp;Wi-Sik Kim","doi":"10.1007/s10499-025-01880-6","DOIUrl":"10.1007/s10499-025-01880-6","url":null,"abstract":"","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}