Aquaculture International最新文献

{"title":"Replacing fishmeal with Palaemon serratus meal enhances growth, carcass traits, digestive enzymes, immune-physiological response, antioxidants, and inner organ histopathology of Dicentrarchus labrax","authors":"Toqa S. M. Saif,&nbsp;Hatem H. Mahmoud,&nbsp;Ashraf I. G. Elhetawy,&nbsp;Reda M. Fahim,&nbsp;Hosam E. Elsaied,&nbsp;Mohamed M. Abdel-Rahim","doi":"10.1007/s10499-025-01997-8","DOIUrl":"10.1007/s10499-025-01997-8","url":null,"abstract":"<div><p>The aquaculture industry is currently facing significant challenges due to the increasing demand for fishmeal (FM), which is an indispensable ingredient in marine aquaculture. This study aims to reduce the reliance on fishmeal in marine fish nutrition and replace it with natural low-trophic animal sources that are more sustainable and of comparable nutritional value to those of fishmeal. Six levels of <i>Palaemon serratus</i> (PS) meal were tested in triplicate: 0, 10, 20, 30, 40, and 50% as a replacement for fishmeal, designated PS0, PS10, PS20, PS30, PS40, and PS50, respectively. A total of 216 apparently healthy <i>Dicentrarchus labrax</i> juveniles (average weight 8.75 ± 0.05 g) were randomly allocated to 18 circular fiberglass tanks. The fish were fed the experimental diet (42.75% protein) for 60 days. The results revealed a better water quality indicator (more oxygen and less ammonia) among the <i>PS</i>-tested treatments. The groups fed <i>PS</i>-supplemented diets outperformed the control group in terms of growth and feed utilization indicators, with the PS40 group performing best. A polynomial second-order regression model was employed to get the optimal results in diets which containing 42.5% PS meal for SGR and 38.5% PS for FCR. Compared with those in the PS0 group, immune-related parameters such as IgM, total protein, and total albumin increased by 18.1%, 38.5%, and 41.4%, respectively. The serum lipid profile was better in the PS30 and PS40 groups, whereas digestive enzymes were better in the PS40 and PS50 groups. <i>PS</i> replacement increased serum growth and immune hormone levels. Intestinal and liver histomorphology revealed that increasing the <i>PS</i> replacement rate enhanced sea bass health. Sea bass diets are recommended to replace 40% of fishmeal with <i>P. serratus</i> meal.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-025-01997-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifaceted analysis of cymothoids parasitism, environmental stressors and heavy metal bioaccumulation dynamics in mediterranean farmed fish in Egypt","authors":"Mohamed A. Atwah,&nbsp;Mohsen A. Khormi,&nbsp;Awad A. Abdelbaky,&nbsp;Ola Hasan Abd El Megeed,&nbsp;Ali M. Aborasain,&nbsp;Mai A. Salem,&nbsp;Mohamed A. Soliman,&nbsp;Hanan S. Khalefa,&nbsp;Hanan H. Abd-El Hafeez,&nbsp;Mohamed Abdelsalam","doi":"10.1007/s10499-025-02037-1","DOIUrl":"10.1007/s10499-025-02037-1","url":null,"abstract":"<div><p>This study investigated the interactions between cymothoids parasitism, environmental stressors, and heavy metal bioaccumulation in commercially farmed fish in the Deeba Triangle area, Egypt. Over 600 fish, comprising gilthead seabream (<i>Sparus aurata</i>) and two mullet species (<i>Mugil cephalus</i> and <i>Liza ramada</i>), were examined during spring and summer 2023. Seasonal variations in cymothoids parasite infestation showed higher prevalence during spring (<i>S. aurata</i>: 37%, mullets: 100%) compared to summer (<i>S. aurata</i>: 20%, mullets: 80%). Morphological and molecular characterization identified the parasite as <i>Livoneca redmanii</i>, with mitochondrial 16S rRNA sequencing revealing 98.73% similarity among isolates (PQ580903-PQ580904). Water quality analysis of aquaculture ponds showed suboptimal conditions compared to the source irrigation canal, including elevated unionized ammonia (0.024–0.042 mg/L vs. 0.012 mg/L) and reduced dissolved oxygen (4.8–5.2 mg/L vs. 6.8 mg/L). Heavy metal accumulation followed consistent tissue-specific patterns (liver &gt; gills &gt; muscle) across five metals (Pb, Fe, Cu, Cd, Mn), with <i>M. cephalus</i> demonstrating the highest bioaccumulation potential. In <i>M. cephalus</i> liver tissue, transfer factors for Cu (175) and Fe (15.45) indicated significant metal retention. Correlation analysis in <i>M. cephalus</i> tissues revealed strong positive relationships between Cu-Fe (<i>r</i> = 0.88) and Fe–Mn (<i>r</i> = 0.78). The human health risk assessment indicated that muscle tissue consumption across all species was within safe limits (THQ ≤ 1.0). This integrated analysis provides insights into species-specific management strategies in Mediterranean aquaculture.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-025-02037-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Larviculture of the purple sea urchin Heliocidaris crassispina in artificial and natural seawater","authors":"Elizaldy Acebu Maboloc,&nbsp;James Kar-Hei Fang","doi":"10.1007/s10499-025-02031-7","DOIUrl":"10.1007/s10499-025-02031-7","url":null,"abstract":"<div><p>Artificial seawater has been utilised as an alternative culture medium in the larval rearing of various marine organisms. However, the viability and the effects of artificial seawater on the early-life stages of echinoderms have not been extensively studied. In this study, we examined the impacts of artificial seawater on the larval development, physiology, metamorphosis, and settlement of the purple sea urchin <i>Heliocidaris crassispina</i>, which is an ecologically and economically important seafood native in Hong Kong waters. Our results showed that larvae cultured in artificial seawater exhibited a 31% reduction in growth rates with more than 60% increase in respiration rates and more than 80% increase in frequency of larval deformities. Larval settlement was not delayed but only 0.5% settled. Larval mortality was significantly high in artificial seawater with less than 1% compared to 14% survival in natural seawater. The differences in salt composition and carbonate chemistry between artificial and natural seawater may have detrimental effects on the sensitive developmental stages of <i>H. crassispina</i>. Nevertheless, given the increasing pollution risks affecting natural seawater quality and reliability, continued research into developing optimised artificial seawater formulations with improved ecological compatibility remains a crucial avenue for sustainable <i>H. crassispina</i> seedling production.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-025-02031-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The continuous application of peracetic acid and UV-C irradiation in rainbow trout aquaculture—a case study under a multifocal pathogen event in a commercial flow-through fish farm","authors":"Christopher Naas,&nbsp;Heike Schmidt-Posthaus,&nbsp;Ramona Lüthi,&nbsp;Alexander Kappe","doi":"10.1007/s10499-025-01980-3","DOIUrl":"10.1007/s10499-025-01980-3","url":null,"abstract":"<div><p>In order to investigate the effects on fish production and health in a commercial aquaculture facility, rainbow trout (<i>Oncorhynchus mykiss</i>) with an initial average body weight of 41 g were reared in raceway-channels under a continuous application of either peracetic acid (0.2 mg PAA L⁻¹) or ultraviolet C light (UV-C, 316.7–155.8 mWs cm⁻²) to the inflowing water for a total of 183 days. Compared to the control, all performance metrics recorded in the fish from the PAA and UV-C treatment groups were significantly increased at the end of the production. To account for the multi-pathogen exposure in the fish farm, regular fish health examinations were conducted. Pathogens detected were the infectious pancreatic necrosis virus (IPNV), <i>Flavobacterium psychrophilum</i>, <i>Aeromonas salmonicida</i>, and <i>Tetracapsuloides bryosalmonae</i>, the causative agent of proliferative kidney disease (PKD). Furthermore, rainbow trout displayed symptoms of puffy skin disease (PSD). From the perspective of PSD, the PAA protocol reduced the prevalence and severity of the disease. At the end of the production cycle, 90% of fish from the PAA treatment remained marketable. Thus, PAA can be considered a promising tool to protect rainbow trout from PSD in the future. In contrast, UV-C light did not soothe or prevent PSD outbreaks. However, the UV-C treatment delayed the development of PKD symptoms in the corresponding group by around 1 month. The application of the treatment methods investigated resulted in increased production costs. Overall, the continuous application of the pathogen reduction methods proved to be positive for fish health and by this for aquaculture.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary betaine on growth, physiological response, and immune-related genes in Chinese mitten crab (Eriocheir sinensis)","authors":"Nouran Mahmoud Sayed,&nbsp;Hesham Eed Desouky,&nbsp;Kenneth Prudence Abasubong,&nbsp;Hossam-eldin Seddik,&nbsp;Ziping Zhang","doi":"10.1007/s10499-025-02039-z","DOIUrl":"10.1007/s10499-025-02039-z","url":null,"abstract":"<div><p>A 2-month study investigated the effects of dietary betaine on growth, physiological response, and immune-related genes in Chinese Mitten Crab (<i>Eriocheir sinensis</i>). A total of 160 crabs, averaging 43.35 ± 0.05 g, were randomly assigned to 16 cement pools, with four pools per treatment and ten crabs per pool. Experimental diets included a control (no betaine) and betaine supplements at 0.5, 1.0, and 1.5 g/kg, respectively. From the results, crabs fed 1 g/kg betaine had higher final weight (FW), specific growth rate (SGR), feed intake (FI), and meat yield (MY), with lower FCR than the control. No significant differences were observed with the 1.5 g/kg supplement. Dietary treatment did not affect Alanine aminotransferase enzyme (ALT), total cholesterol (TC), triglycerides (TG), or non-esterified fatty acids (NEFA). However, aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) activities were significantly reduced in crabs fed betaine, with the lowest levels in those fed 1 and 1.5 g/kg in hemolymph and hepatopancreas. Superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) activities in the hepatopancreas were significantly higher than in the control, with opposite trends in malondialdehyde (MDA) content, but no differences were observed in crabs fed 1 g/kg. Crabs fed 1 and 1.5 g/kg betaine had higher lysosomes in the hepatopancreas than the control. Similar results were observed in hemolymph. <i>alf1</i> was upregulated with increasing betaine, peaking in crabs fed 1.5 g/kg, but showed no difference at 1 g/kg. <i>alf2</i> increased with betaine, then decreased at higher levels, with no significant difference between the 0.5 g/kg and the control. Lastly, <i>alf3</i> and <i>crus1</i> were significantly upregulated in crabs fed betaine compared to the control. This study suggests that dietary betaine supplementation at the level of 1 g/kg is the most effective.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144100364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of season, culture system, and stocking density on growth performance and limb loss in blue swimming crab (Portunus pelagicus) from instar to early juvenile stage","authors":"Jenalyn Silvestre Lames,&nbsp;Rex Ferdinand Traifalgar,&nbsp;Fe D. Parado-Estepa,&nbsp;Erish Estante-Superio,&nbsp;Sheryll Santander Avanceña,&nbsp;Vicente Balinas","doi":"10.1007/s10499-025-02029-1","DOIUrl":"10.1007/s10499-025-02029-1","url":null,"abstract":"<div><p>The study investigated the growth, survival rates, and limb loss occurrence of blue swimming crabs (BSC), <i>Portunus pelagicus</i> from crab instar to the early juvenile stage at different culture systems (outdoor tanks, net cages in ponds, and net cages in pens), and varying stocking densities (30, 50, and 100 individuals/m²). These variables were assessed during the wet and dry seasons for 21 days following a 2 × 3x3 factorial design. Survival showed a significant interaction between culture systems and seasons (p &lt; 0.001). However, no significant differences were found between culture systems and stocking densities, or between stocking densities and seasons (<i>p</i> &gt; 0.05). Results showed that pond and pen systems, particularly at moderate densities (30–50 individuals/m²), yielded significantly higher growth, survival, and biomass production compared to tank systems (p &lt; 0.05). Survival rates were significantly higher in ponds (76.69% ± 2.58) than in pens (54.97% ± 2.10), and tanks (38.32% ± 4.61). Moreover, BSC juveniles exhibited limb loss (%) ranging from 11.43% to 35.17%, with loss of a single limb being the most common, occurring in 49–70% of the crabs. Overall, the study recommends the use of pond and pen systems with moderate stocking densities for optimal nursery production of <i>P. pelagicus</i>, contributing valuable insights for improving aquaculture protocols and supporting stock enhancement initiatives toward sustainable fisheries.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of growth performance and metabolic efficiency in Labeo rohita fed a sorghum-based diet supplemented with chromium chloride hexahydrate","authors":"Gulnaz Zia,&nbsp;Rehana Iqbal,&nbsp;Hamid Manzoor,&nbsp;Muhammad Afzaal,&nbsp;Muhammad Khalid","doi":"10.1007/s10499-025-02027-3","DOIUrl":"10.1007/s10499-025-02027-3","url":null,"abstract":"<div><p>The increasing costs of fish feed in the aquafeed industry have highlighted the need to explore non-conventional carbohydrate sources that can sustain fish growth and health. Integrating carbohydrates with chromium compounds has emerged as a viable strategy to enhance energy efficiency and improve the overall health of fish. To explore this, sorghum was utilized as the carbohydrate source in a 90-day feeding trial designed to evaluate the impact of Cr₂Cl₃•6H₂O supplementation on the growth performance and metabolic parameters of <i>Labeo rohita</i>. Four experimental diets were formulated, containing 0, 0.3, 0.5, and 0.7 mg of Cr₂Cl₃•6H₂O per kilogram, labeled as D1, D2, D3, and D4, respectively. Different growth parameters, such as percentage weight gain, specific growth rate (SGR), protein efficiency ratio (PER), and body length, exhibited optimum values, while feed conversion ratio (FCR) recorded the lowest value at 0.3 mg Cr₂Cl₃•6H₂O/kg diet. Moreover, a significant quadratic relationship (<i>p</i> &lt; 0.001) between Cr₂Cl₃•6H₂O supplementation and SGR identified an optimal inclusion level of 0.345 mg/kg in feed formulation. The digestive enzymes amylase and protease activity increased in D2 treatment. The liver cholesterol and triglyceride levels decreased at 0.3 mg Cr₂Cl₃•6H₂O/kg, while the highest level of high-density lipoprotein (HDL) and the lowest level of low-density lipoprotein (LDL) were observed at 0.5 mg Cr₂Cl₃•6H₂O/kg in the diet. The glycogen contents increased; urea and creatine contents decreased at 0.3 mg Cr₂Cl₃•6H₂O/kg. Insulin activity increased by addition of Cr. The enzymes PEP kinase and FDPase activity increased up to 0.5 mg while G6PDH at 0.3 mg Cr₂Cl₃•6H₂O/kg; then, activity of these enzymes decreased by further addition of Cr. The minimum value of SOD was at 0.3 mg Cr₂Cl₃•6H₂O/kg, ALT and AST values at 0.5 mg Cr₂Cl₃•6H₂O/kg diet while gill ATPase activity slightly increased by increasing the Cr in fish feed. The results indicated that the optimal inclusion level of Cr₂Cl₃•6H₂O in a sorghum-based diet was 0.3–0.5 mg/kg, which supported superior growth performance, enhanced digestibility, and improved metabolic efficiency in fish.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary supplementation of Lactiplantibacillus plantarum probiotics as functional feed additive in Pacific white shrimp (Peneaus vannamei)","authors":"Natarajan Lalitha,&nbsp;Kondusamy Ambasankar,&nbsp;Sivaramakrishnan Thirugnanamurthy,&nbsp;Sherly Tomy,&nbsp;Panjan Nathamuni Suganya,&nbsp;Ramalingam Ananda Raja,&nbsp;Sujeet Kumar,&nbsp;Raman Nanthini,&nbsp;Oimps Lunghar","doi":"10.1007/s10499-025-02023-7","DOIUrl":"10.1007/s10499-025-02023-7","url":null,"abstract":"<div><p>The current study aimed to investigate <i>Lactiplantibacillus plantarum</i> as candidate probiotic and dead bacteria functional aquafeed additives in <i>P</i>. <i>vannamei</i>. The 45-day feeding experiment was carried out in juvenile whiteleg shrimp, <i>P. vannamei</i>. Two hundred and seventy shrimps were randomly distributed into nine tanks with 30 juvenile shrimps (initial body weight 4.43 ± 0.045 g) constituting three replicates per treatment. Water temperature was maintained at 26–29 °C. Three experimental diets were formulated, viz. basal diet with live <i>L. plantarum</i> 10¹¹ CFU Kg⁻¹ of feed (LLP); basal diet with <i>L. plantarum</i> dead bacteria (DLP); and control basal diet (CON). The animals were fed to satiation and the feed was offered at 6% of the body weight. The diet contains 364 g/kg crude protein and 15.79 MJ/kg energy level. It was found that the group fed with LLP showed higher (17.03 ± 0.42 g) final weight, weight gain, SGR, PER, and survival than DLP and CON, though statistically not significant. However, the PPV was significantly (<i>P</i> &lt; 0.05) higher in the LLP-fed group. Significantly (<i>P</i> &lt; 0.05) higher total haemocyte count; small and large nongranular haemocyte; and small and large granular haemocyte, as well as the percentage occurrence of these cells, were observed in LLP. Further, prophenol oxidase enzyme was significantly (<i>P</i> &lt; 0.05) high in LLP and DLP. The immune-related gene <i>hsp 70</i> was significantly expressed higher (<i>P</i> &lt; 0.05) in LLP and DLP. The LLP group recorded significantly (<i>P</i> &lt; 0.05) higher amylase (1.80 ± 0.36 U mg⁻¹) and leucine aminopeptidase (0.78 ± 0.152 U mg⁻¹) hepatopancreatic digestive enzyme activity. Midgut depicted significantly (<i>P</i> &lt; 0.05) high crypt depth (23.68 µm ± 8.20), villi height (59.53 µm ± 10.42), and muscular layer thickness (91.92 µm ± 13.98) in LLP. <i>Rhodobacteraceae</i> (37.69%) and <i>Flavobacteriaceae</i> (15.74%) were beneficial core microbiome bacterial signatures observed predominantly in LLP. In challenge studies with <i>Vibrio campbellii</i>, the LLP group recorded significantly (<i>P</i> &lt; 0.05) lower cumulative mortality (30%) and significantly (<i>P</i> &lt; 0.05) upregulated <i>prophenol oxidase</i>, <i>super oxide dismutase</i>, and <i>heat shock protein</i> immune gene expression. The results of the current study infer that dietary supplement of <i>L. plantarum</i> at 3% would act as a functional feed additive in the diet of <i>P. vannamei</i>.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salinity and alkalinity as potential controls for pCO2 reduction in an experimental shrimp (Litopenaeus vannamei) farming","authors":"Andréa da Consolação de Oliveira Carvalho,&nbsp;Mariany Sousa Cavalcante,&nbsp;Alberto Nunes,&nbsp;João Felipe Nogueira Matias,&nbsp;Rozane Valente Marins","doi":"10.1007/s10499-025-02010-y","DOIUrl":"10.1007/s10499-025-02010-y","url":null,"abstract":"<div><p>Shrimp farming, once limited to coastal areas, is now thriving in non-coastal regions as well, due to the success of the whiteleg shrimp (<i>Litopenaeus vannamei</i>) cultivation across a wide range of salinity. The purpose of this study was to evaluate the influence of salinity on the CO₂-carbonate system during experimental shrimp farm cultivation aiming sustainable crustacean aquaculture. The evaluation involved two shrimp production systems, where water samples were collected periodically from seven tanks of hypersaline water (M1) and seven tanks of brackish water (M2), making a total of fourteen semi-closed experimental units. Both treatments used the same feeding method. Carbonate system parameters (TA and pH) and ancillary data (including temperature, salinity, dissolved oxygen, and phosphorus fractions) were all sampled during the rearing cycle of these aquatic organisms. Additionally, pCO₂ and dissolved inorganic carbon (DIC) were estimated for a complete evaluation of CO₂-carbonate system parameters. The results indicate that the hypersaline treatment (M1) resulted in lower pCO₂. In contrast, the mesohaline treatment (M2) led to higher pCO₂. The total alkalinity (TA) varied from 2420 to 3469 µmol. kg-1 in M1 and from 3589 to 4463 µmol. kg-1 in M2. Since the addition of lime is a common practice in aquaculture, our results suggest that it also should be managed as an approach for removing CO₂ in shrimp farming, besides the use of hypersaline waters, or to improve the carbon sustainability of brackish water shrimp farms, in search for greater sustainability of this activity in accordance with Sustainable Development Goal 14 – Life Below Water.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A common garden comparison of the microbiome and gene expression of intertidally- and subtidally-cultured Pacific oysters (Crassostrea gigas) in relation to extreme warming events","authors":"Clara L. Mackenzie,&nbsp;Monique R. Raap,&nbsp;Colleen T. E. Kellogg,&nbsp;Chen Yin V. Walker,&nbsp;Rute B. G. Clemente-Carvalho,&nbsp;Timothy J. Green,&nbsp;Christopher M. Pearce","doi":"10.1007/s10499-025-01959-0","DOIUrl":"10.1007/s10499-025-01959-0","url":null,"abstract":"<div><p>In order to investigate how shellfish culture in the intertidal zone may serve as a means of conferring resilience prior to subtidal deployment for commercial grow-out, a common garden experiment was carried out with juvenile Pacific oysters. Individuals were cultured under intertidal or subtidal conditions for one year, following which intertidal animals were transplanted to the subtidal site and both treatment groups were cultured together under subtidal conditions for a period of five months. During that time, microbiome and gene expression were tracked in relation to a marine heatwave event. Multivariate analyses of samples indicated a significant effect of time on gene expression, but no significant effect of culture treatment prior to and during the heatwave. Significant differences in the expression of oxidative stress and immune response genes in post-heatwave samples in comparison to pre-heatwave and heatwave samples, and between culture treatment groups post-heatwave, were detected. Results also indicated significant differences in microbiome according to culture treatment and timepoint, with increased richness observed in intertidally-cultured oysters and over time. Similarly, while the overall composition of the oyster microbiome did not vary between culture treatments, changes were observed over time. At the end of the field trial, a laboratory-based temperature and <i>Vibrio</i> challenge experiment was conducted to compare genomic responses of both culture treatment groups following 24-h exposure to coinciding acute warming and pathogen stressor conditions. A significant interactive effect of temperature and culture treatment on gene expression was observed, further suggesting that stress response may be influenced by prior culture treatment.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-025-01959-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}