Aquaculture International最新文献

{"title":"Dietary effects of coenzyme Q10 and its micelle form on growth, immunity, antioxidant capacity and disease resistance to Vibrio parahaemolyticus in Pacific white shrimp (Penaeus vannamei)","authors":"Gunho Eom,&nbsp;Wonhoon Kim,&nbsp;Mirasha Hasanthi,&nbsp;Nalin Medagoda,&nbsp;Kwan-Sik Yun,&nbsp;Kyeong-Jun Lee","doi":"10.1007/s10499-026-02709-6","DOIUrl":"10.1007/s10499-026-02709-6","url":null,"abstract":"<div><p>Coenzyme Q10 (CQ) is a lipid-soluble bioactive compound involved in mitochondrial energy production and antioxidant defense. This study evaluated the effects of dietary CQ (99.9% purity) and its micelle form (MQ, 3.5% purity) on <i>Penaeus vannamei</i>. A basal diet (Con) was supplemented with CQ by 20 and 40 mg kg⁻¹ (CQ2 and CQ4, respectively) and MQ by 10, 20, 40, 80 and 160 mg kg⁻¹ (MQ1, MQ2, MQ4, MQ8 and MQ16, respectively). Triplicate groups of shrimp (initial body weight, 0.70 ± 0.01 g) were fed their respective diets for 56 days. Growth performance was improved in CQ2 and MQ2‒MQ16 groups compared with Con group. Dietary supplementation of CQ and MQ increased CQ and vitamin E levels in hepatopancreas and muscle tissues. Trypsin and chymotrypsin activities were higher in CQ2‒CQ4 and MQ8‒MQ16 groups, while lipase activity was higher in all CQ- and MQ-supplemented groups. CQ and MQ supplementation increased the number of blasenzellen, regenerative cells and lipid droplets in hepatopancreatic tubules. Non-specific immune responses were enhanced in MQ8 and MQ16 groups, whereas antioxidant capacity in CQ4 and MQ2‒MQ16 groups compared to Con group. Dietary MQ supplementation significantly increased the abundance of lactic acid bacteria while reducing <i>Vibrio</i> abundance in the gut. During a <i>V. parahaemolyticus</i> challenge, survival was significantly higher in CQ4 and MQ2‒MQ16 groups. Based on these findings, CQ may serve as a functional feed additive for shrimp, while micelle form contributes to improved absorption and bioavailability. Thus, the dietary MQ supplementation level at 80‒160 mg kg⁻¹ is recommended for <i>P. vannamei</i>.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The beneficial effects of nanoparticles loaded with plant essential oils on growth performance, immunity, health status, and bacterial pathogens in sustainable fish farming: a comprehensive review","authors":"Mustafa Shukry,&nbsp;Gehad E. Elshopakey,&nbsp;Amany M. Shosha,&nbsp;Ahmed E. A. Mostafa,&nbsp;Ibrahim Albokhadaim,&nbsp;Samir Alhojaily,&nbsp;Ahmed O. Alameen,&nbsp;Samia Elbahnaswy","doi":"10.1007/s10499-026-02690-0","DOIUrl":"10.1007/s10499-026-02690-0","url":null,"abstract":"<div><p>Nanotechnology is a promising strategy for enhancing the efficacy of nerolidol. Recent nutritional approaches focus on using nanoparticles (NPs) functionalized with herbal essential oils (EOs) to enhance growth, immunity, and overall physiology in fish species; accordingly, the benefits of encapsulated EOs as dietary supplements have been reported in cultured aquatic animals. There is growing interest in functional nanoparticles due to their versatile properties and size, which enable targeted administration and controlled release of bioactive substances from plant essential oils. The antibacterial and antioxidant properties of encapsulated EOs, along with their role in promoting intestinal health, have also attracted increased interest in fish production because of their potent efficacy against susceptible bacterial fish pathogens, their nutritive value in improving fish growth and feed utilization, and their role as modulators of the gut bacterial community. This review summarizes the current knowledge regarding the use of plant EOs-loaded nanoparticles as fish dietary additives. However, several challenges must be addressed to fully unravel their potential dosages to sustain fish health and performance under environmental stressors. Additionally, most encapsulation techniques are emerging approaches for controlling EO stability and release in aquatic feeds. However, further research is needed to refine these approaches and evaluate their scalability. This review also aims to examine emerging trends in the application of nanotechnology to enhance the efficacy of herbal essential oils for controlling bacterial infections in cultured fish species. This is related to their potential efficiency against susceptible, multidrug-resistant, and biofilm-embedded microorganisms. A key feature of this review is the systematic presentation of detailed, species-specific tables summarizing the current literature on the application of essential oils encapsulated with nanoparticles in aquaculture.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allicin E enhances the antibacterial activity of enrofloxacin against Aeromonas hydrophila in Carassius auratus gibelio","authors":"Liushen Lu,&nbsp;Jinlong Li,&nbsp;Ting Qin,&nbsp;Kai Chen,&nbsp;Jun Xie,&nbsp;Fang Ping,&nbsp;Bingwen Xi","doi":"10.1007/s10499-026-02710-z","DOIUrl":"10.1007/s10499-026-02710-z","url":null,"abstract":"<div><p><i>Aeromonas</i> spp. are major pathogens severely affecting the <i>Carassius auratus gibelio</i> farming industry. The prolonged use of enrofloxacin (Enr) has led to reduced therapeutic efficacy, drug residues, and the emergence of antibiotic resistance. Simply increasing antibiotic dosage is unsustainable, and the core challenge lies in potentiating antibiotic efficacy without dose escalation. Here, we employed combination therapy using allicin E (ALE) as a potentiator of Enr and investigated its synergistic antibacterial activity, underlying mechanisms, and protective effects against <i>A. hydrophila</i> in <i>C. auratus gibelio</i>. In vitro, the combination of Enr and ALE exhibited broad-spectrum synergistic or additive activity against multiple aquatic pathogens, rapidly eradicating <i>A. hydrophila</i> without regrowth during the observation period. Mechanistically, the combination triggered a burst of bacterial reactive oxygen species (1.67-fold vs. control), leading to irreversible membrane damage and leakage of intracellular contents, and significantly inhibited biofilm formation and disrupted mature biofilms. In vivo, oral administration of the combination in <i>C. auratus gibelio</i> challenged with <i>A. hydrophila</i> significantly improved survival (66.6% vs. 26.6% in model), reduced bacterial loads in liver, spleen and kidney (by 70.9%, 81.3% and 72.9%, respectively, vs. model group), and mitigated oxidative damage by restored antioxidant enzymes (T-SOD, CAT) activity, decrease MDA, as well as attenuated inflammatory infiltration and hemorrhage in spleen and kidney tissues. This is the first report of ALE potentiating Enr against <i>A. hydrophila</i> via a synergistic ROS-mediated membrane damage, validated in <i>C. auratus gibelio</i>, with approximately 50% Enr reduction potential.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential regulatory mechanisms underlying papillae number in Apostichopus japonicus: an integrative genomic and transcriptomic analysis","authors":"Fengqin Wang,&nbsp;Yibo Wang,&nbsp;Zixin Hong,&nbsp;Jian Zhang,&nbsp;Xiuyun Wang,&nbsp;Shanshan Yu,&nbsp;Qinglin Wang,&nbsp;Chunlong Zhao","doi":"10.1007/s10499-026-02680-2","DOIUrl":"10.1007/s10499-026-02680-2","url":null,"abstract":"<div><p><i>Apostichopus japonicus</i> is an economically important marine species in China, where the number of papillae constitutes a key phenotypic trait that directly influences body wall proportion and, consequently, market value. In this study, an integrated analytical approach combining genome-wide association studies (GWAS) with transcriptomic profiling was first employed to identify genetic regulatory regions associated with papillae number in <i>A. japonicus</i>. Four single nucleotide polymorphisms (SNPs) and 27 potential candidate genes were identified through GWAS. Transcriptome sequencing revealed 68 differentially expressed genes, comprising 32 upregulated and 36 downregulated genes. Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that these differentially expressed genes were significantly enriched in ion transmembrane transport, apoptosis, and neural network development. Integrated analysis of whole-genome and transcriptomic data identified several candidate genes—<i>evm.TU.Chr5.107</i>, <i>evm.TU.Chr5.103</i>, <i>evm.TU.Chr5.111</i>, <i>evm.TU.Chr5.109</i>, <i>evm.TU.Chr5.97</i>, <i>evm.TU.Chr5.98</i> and <i>evm.TU.Chr6.166</i>, that are potentially involved in regulating papillae development via immune-related functions, cellular metabolic processes and neural protection. qRT-PCR validation further substantiated the involvement of <i>evm.TU.Chr5.103</i> and <i>evm.TU.Chr5.107</i> in the regulation of papillae number. These results enhance our understanding of the molecular mechanisms governing papillae formation and offer promising molecular markers for selective breeding programs aimed at increasing papillae abundance in <i>A. japonicus</i> strains.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Sargassum powder enhances growth, hematological profiles, intestine morphology, and disease resistance in Oreochromis niloticus","authors":"Md Imtiaz Ahamed,&nbsp;Md. Sakhawat Hossain,&nbsp;Maria Tabasoom Pingki,&nbsp;Nusrat Jahan Sumaiya,&nbsp;Nayan Jyoti Roy Kanok,&nbsp;A. K. Shakur Ahammad,&nbsp; Mariom,&nbsp;Mohammad Matiur Rahman","doi":"10.1007/s10499-026-02699-5","DOIUrl":"10.1007/s10499-026-02699-5","url":null,"abstract":"<div><p><i>Sargassum</i> spp. are increasingly valued for their bioactive compounds and aquaculture applications. The present study evaluated the effects of dietary <i>Sargassum</i> powder supplementation on growth, feed utilization, body indices, hematological parameters, intestinal histomorphology, and disease resistance of <i>Oreochromis niloticus</i> reared in cistern tanks. A total of 600 fish were randomly distributed among four dietary treatments (T1: 0%, T2: 2%, T3: 4%, and T4: 8% <i>Sargassum</i>), with three replicate tanks per treatment, and reared for three months. The results demonstrated that fish fed with 2% <i>Sargassum</i> diet (T2) exhibited significantly (<i>p</i> &lt; 0.05) higher growth performance than those in all other dietary treatments (T1, T3, and T4), with the highest weight gain (65.49 ± 1.76 g) and specific growth rate (2.55 ± 0.04). Feed utilization was most efficient in T2, as indicated by its significantly (<i>p</i> &lt; 0.05) lower feed conversion ratio (1.27 ± 0.03) compared with those of all other treatments. Among hematological parameters, fish receiving 2% <i>Sargassum</i> exhibited significantly (<i>p</i> &lt; 0.05) elevated red blood cell, white blood cell, hemoglobin, and packed cell volume values compared to all other groups. Histomorphological analysis indicated that 2% <i>Sargassum</i> supplementation significantly (<i>p</i> &lt; 0.05) improved intestinal morphology compared with the control group (T1). Following an intraperitoneal challenge with <i>Aeromonas hydrophila</i>, T2 exhibited a significantly (<i>p</i> &lt; 0.05) higher survival rate (90%) compared with the control group (T1). Overall, dietary inclusion of 2% <i>Sargassum</i> significantly improved growth performance, feed utilization, intestinal morphology, and disease resistance in <i>Oreochromis niloticus</i>, supporting its potential as a sustainable feed additive.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149133257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-encoder transformer with dual-branch co-attention mechanism for growth prediction in largemouth bass","authors":"Yufei Ren,&nbsp;Xuhui Duan,&nbsp;Shaohua Chen,&nbsp;Fuli Zhao,&nbsp;Yan Lei,&nbsp;Daoliang Li,&nbsp;Chen Shi","doi":"10.1007/s10499-026-02696-8","DOIUrl":"10.1007/s10499-026-02696-8","url":null,"abstract":"","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149133188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary citric acid-supplemented Moringa oleifera seed meal-based diets promotes growth, hematology, and nutrient digestibility in Labeo rohita fingerlings","authors":"Majid Hussain,&nbsp;Nida Ismat,&nbsp;Adan Naeem,&nbsp;Hesham A. Hassanien,&nbsp;Ahmed O. Abbas,&nbsp;Abdallah Tageldein Mansour","doi":"10.1007/s10499-026-02705-w","DOIUrl":"10.1007/s10499-026-02705-w","url":null,"abstract":"<div><p>Citric acid (CA), an organic acid used as an acidifier in aquafeeds, has been reported to improve growth, feed utilization and disease resistance in some aquatic species. It has the potential to facilitate the partial replacement of fishmeal with plant-based by-products by enhancing their nutrient utilization and overall nutritional efficiency. Therefore, this feeding trial was conducted to determine the effects of graded levels of CA supplementation in <i>Moringa oleifera</i> seed meal (MOSM)-based diets on growth parameters, hematology, and apparent nutrient digestibility in <i>Labeo rohita</i> fingerlings. A completely randomized design (CRD) was employed, with 270 fingerlings randomly distributed into six groups (15 fish per tank) with three replicates each. Six experimental diets were prepared, with one serving as the control diet containing 0% CA. The other five diets contained 1%, 2%, 3%, 4%, and 5% CA supplementation, respectively. Fish received a basal diet containing MOSM and fed at 5% of their live body weight twice daily. Fish were stocked in tanks for three months. According to results, 3% CA supplementation significantly increased weight gain (268.04%), specific growth rate (1.45), and decreased feed conversion ratio (1.23) (<i>p &lt;</i> 0.05) in comparison with the control and other treatment groups. The inclusion of 3% CA supplemented diets significantly increased (<i>p</i> &lt; 0.05) the blood indices (RBC, Hb, WBC) in contrast to the control and other groups. Moreover, the highest gross energy (66.49%) and crude fat (76.71%) digestibility values were observed in diets supplemented with 3% CA (<i>p</i> &lt; 0.05), while the highest crude protein digestibility (67.49%) was estimated at 4% CA supplemented diets. Conclusively, 3% CA supplemented MOSM-based diets improve the growth, hematological indices, and nutrient absorption in <i>L. rohita</i> fingerlings.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149133304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intestinal microbiota-host hepatopancreas transcriptome interactions in response to decapod iridescent virus 1 (DIV1) infection in Macrobrachium rosenbergii","authors":"Jie Yang,&nbsp;Yuqing Shen,&nbsp;Miuying Cai,&nbsp;Zhenglong Xia,&nbsp;Jiongying Yu,&nbsp;Qiang Sheng,&nbsp;Qiongying Tang,&nbsp;Shaokui Yi,&nbsp;Quanxin Gao","doi":"10.1007/s10499-026-02704-x","DOIUrl":"10.1007/s10499-026-02704-x","url":null,"abstract":"<div><p>Decapod iridescent virus 1 (DIV1) has severely threatened <i>Macrobrachium rosenbergii</i> aquaculture, yet sex-specific crosstalk between intestinal microbiota and hepatopancreatic immunity upon DIV1 infection remains unclear. Here, we performed oral DIV1 challenge on male and female prawns, integrating 16S rDNA sequencing and hepatopancreas transcriptomics to decode sex-disparate antiviral responses. The 16S rDNA results show that early infection (day 8) leads to an increase in microbiota diversity. By day 12, the diversity recovered in female <i>M. rosenbergii</i>, whereas it remained high in males, indicating stronger intestinal immunity in females. At the phylum level, the relative abundance of Firmicutes increased in females but decreased in males, and Pseudomonadota was more abundant in females than in males. <i>Lactococcus</i> tended to reach peak relative abundance at the early infection stage (day 8), whereas <i>Enterobacter</i> was rarely detected at Day 8 and only emerged at the late stage (Day 12), indicating distinct temporal succession patterns of these two genera during DIV1 infection. Transcriptome analysis indicated that females showed progressive immune activation: early oxidoreductase activity, mid-stage carbohydrate metabolism/reactive oxygen species (ROS) scavenging, and late-stage epigenetic and tissue repair. Males continuously activated the lysosomal and glutathione pathways. Key genes (<i>cathepsin L-like</i>, <i>ARSB</i>, <i>Glo1</i>) were differentially expressed in females, suggesting their potential roles in immune regulation and antioxidant defense responses. The microbiota-host correlation highlights sex-specific interactions: <i>CSADL1</i> in females (associated with the microbiota) may enhance antioxidant capacity, while <i>Gpat4</i>, a lipid metabolism-related gene in males, is negatively correlated with the microbiota, reflecting the metabolic-microbiota regulatory association. This study uncovers sex-divergent antiviral response patterns of <i>M. rosenbergii</i> against DIV1, supplying theoretical references and potential biomarker candidates for sex-targeted disease control and selective breeding of resistant prawn strains.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149133305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic evaluation of fermented sugar beet pulp on growth, metabolic responses, antioxidant status, immunity, and physiological health in Labeo rohita","authors":"Areej Farooq,&nbsp;Noor Khan,&nbsp;Muhammad Awais,&nbsp;Faraz Ali,&nbsp;Syed Zakir Hussain Shah,&nbsp;Shaista Nawaz","doi":"10.1007/s10499-026-02706-9","DOIUrl":"10.1007/s10499-026-02706-9","url":null,"abstract":"<div><p>This study evaluated the use of fermented sugar beet pulp (FSBP) as a feed ingredient for juvenile <i>Labeo rohita</i> (initial weight, 15 g). A completely randomized design (CRD) with seven dietary treatments and three replicates per treatment was used for a 12-week feeding trial. The diets included a control (0% sugar beet pulp, SBP), three treatments with raw SBP (RSBP) at 7%, 14%, and 21%, and three treatments with FSBP at the same inclusion levels. Fermentation was carried out using liquid baker’s yeast waste as the fermenting agent. After the feeding trial, growth performance and whole-body composition were assessed. Whole-blood, serum, liver, and intestinal samples were analyzed for hematological indices, biochemical parameters, antioxidant enzyme activities, and digestive enzyme activities, respectively. Fish fed the 21% FSBP diet (T6) showed the highest final body weight, net weight gain, specific growth rate, feed efficiency, and protein efficiency ratio, together with the lowest feed conversion ratio, compared with fish fed the control and RSBP-based diets (<i>p</i> &lt; 0.05). Whole-body crude protein content was significantly higher in fish fed the 21% FSBP diet than in all other dietary groups (<i>p</i> &lt; 0.05). Hepatic CAT, GPx, and SOD activities and intestinal protease, amylase, and lipase activities were highest in fish fed T6 and lowest in fish fed the 21% RSBP diet (T3) (<i>p</i> &lt; 0.05). Whole-blood Hb, RBC count, HCT, WBC count, and platelet count were significantly higher in fish fed T6 than in fish fed T3, whereas serum ALT, AST, ALP, and LDH activities and triglyceride concentration were significantly lower, and total protein concentration was significantly higher in T6 than in T3 (<i>p</i> &lt; 0.05). Collectively, these findings indicate that fermentation enhanced the nutritional profile and health-related benefits of SBP, with FSBP supplementation up to 21% improving growth, antioxidant response, and liver health without adverse effects on the parameters assessed. FSBP presents a cost-effective raw material for use in aquafeeds, supporting enhanced <i>L. rohita</i> production.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149133306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global advancements in anuran breeding: a mini-review of the integration of conservation biotechnologies and commercial aquaculture","authors":"Yinglin He,&nbsp;Hao Ding,&nbsp;Yanfang Pan,&nbsp;Zhuo Chen,&nbsp;Fang Zeng,&nbsp;Chao Xu,&nbsp;Huirong Yang","doi":"10.1007/s10499-026-02707-8","DOIUrl":"10.1007/s10499-026-02707-8","url":null,"abstract":"<div><p>This review synthesizes the state of global anuran breeding research as of early 2026, highlighting a dual mandate: the urgent conservation of biodiversity amidst the global amphibian crisis and the sustainable intensification of commercial aquaculture. While these fields have historically operated in parallel, they increasingly share critical methodologies. We examine advancements in assisted reproductive technologies (ARTs), particularly the refinement of hormonal induction protocols and the widespread adoption of computer-assisted sperm analysis (CASA). Significant attention is given to the cryobiological bottleneck; while sperm cryopreservation is now a robust tool for biobanking, the preservation of oocytes remains elusive due to biophysical challenges such as chilling injury and lipid phase transitions. We discuss the emergence of embryonic cell cryopreservation and somatic cell nuclear transfer (SCNT) as viable alternatives for banking and restoring diploid genomes, exemplified by the Lazarus Project. Furthermore, the review analyzes the genomic revolution in anurans, contrasting the advanced gene-editing capabilities in model <i>Xenopus</i> species with the nascent but accelerating genomic selection in commercial species like the American bullfrog (<i>Aquarana catesbeiana</i>). The integration of these technologies promises to secure genetic diversity for endangered species while optimizing growth and disease resistance in the aquaculture sector.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149133255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}