Aquaculture International最新文献

{"title":"Effects of geosmin and 2-methylisoborneol exposure on the gill transcriptome of Nile tilapia (Oreochromis niloticus)","authors":"Jun-Ming Zhang,&nbsp;Huan Han,&nbsp;Sow Abdoul Gadiri,&nbsp;Wannapha Kitrattana,&nbsp;Zhong-Qian Wang,&nbsp;Jing-Wei Liu,&nbsp;Kang Li,&nbsp;Li-Ping Liu","doi":"10.1007/s10499-025-01859-3","DOIUrl":"10.1007/s10499-025-01859-3","url":null,"abstract":"<div><p>Geosmin (GSM) and 2-methylisoborneol (2-MIB) are two common earthy-musty compounds produced by various microorganisms in aquatic environments that cause off-flavors in fish. However, few studies have explored the molecular responses of fish gills to GSM and 2-MIB exposure. In this study, Nile tilapia (<i>Oreochromis niloticus</i>) were exposed to 10 μg/L of both GSM and 2-MIB for 24 h, after which gill samples were collected for transcriptome sequencing. A total of 65 and 116 differentially expressed genes (DEGs) were identified in the GSM and 2-MIB treatment groups, respectively. Gene Ontology (GO) analysis highlighted biological processes related to metabolism, cellular processes, and catalytic activity. Fifteen DEGs, 12 upregulated and 3 downregulated, were shared between the GSM and 2-MIB treatments. KEGG pathway analysis revealed enrichment in pathways associated with immune response, cell signaling, and metabolism. These findings contribute to a deeper understanding of the molecular mechanisms underlying the effects of GSM and 2-MIB exposure on fish.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Camellia sinensis mediated nanoparticles to control growth and biofilm in Vibrio sp","authors":"Suha Kouser I,&nbsp;Ranjani Soundharajan,&nbsp;Hemalatha Srinivasan","doi":"10.1007/s10499-025-01887-z","DOIUrl":"10.1007/s10499-025-01887-z","url":null,"abstract":"<div><p><i>Camellia sinensis</i> renowned for its medicinal properties has been employed for centuries to address various ailments due to its anti-inflammatory, antioxidant, anti-cancer, and anti-diabetic properties. In this research, <i>Camellia sinensis</i> leaf extract was used for the eco-friendly and cost-effective synthesis of silver nanoparticles (CS-AgNPs). UV–Visible spectroscopy confirmed the synthesis of CS-AgNPs with a surface plasmon resonance band near 350 nm. Fourier-transform infrared (FTIR) analysis revealed functional groups such as alcohols, carboxylic acids, and amines, which acted as reducing, capping, and stabilising agents. Field-emission scanning electron microscopy (FESEM) showcased monodisperse, spherical nanoparticles ranging from 10 to 50 nm, while X-ray diffraction (XRD) confirmed their crystalline structure with prominent diffraction peaks at 38.24°, 44.42°, and 77.40°, corresponding to the (111), (200), and (311) planes of silver’s FCC structure. Zeta potential analysis demonstrated nanoparticle stability, revealing a negative surface charge of − 34.6 mV, which reduced agglomeration through electrostatic repulsion. Dynamic light scattering (DLS) analysis showed an average particle size of 148.6 nm, aligning with their suspension behaviour. Antimicrobial studies exhibited significant efficacy, with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determined at 25 µg/mL and 50 µg/mL, respectively, in tested <i>V. harveyi</i>, <i>V. parahaemolyticus</i>, and <i>V. alginolyticus</i>. The tolerance level of 2 confirms the best bactericidal potential of CS-AgNPs. CS-AgNPs effectively inhibited the biofilm formation at their respective MIC. Comprehensive biochemical assays, including estimating the leakage of proteins, sugars, malondialdehyde (MDA) quantification, glutathione (GSH) measurement, and evaluation of superoxide dismutase (SOD) and catalase (CAT) activities, offered compelling evidence of CS-AgNPs’ robust antibacterial efficacy. This research underscores the transformative capacity of green tea extract, serving as a catalyst for eco-friendly, cost-effective, and reproducible silver nanoparticle synthesis. CS-AgNPs emerge as a potent and economically viable alternative to conventional antibiotics, poised to redefine therapeutic approaches in biomedical science and in aquafarming industries.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing captive maturation and spawning through induced breeding trials in goldlined seabream Rhabdosargus sarba (Forsskål, 1775)","authors":"T. Senthil Murugan,&nbsp;Ezhil P.,&nbsp;Dani Thomas,&nbsp;Aritra Bera,&nbsp;J. Raymond Jani Angel,&nbsp;G. Thiagarajan,&nbsp;M. Kailasam,&nbsp;Kuldeep K. Lal","doi":"10.1007/s10499-025-01871-7","DOIUrl":"10.1007/s10499-025-01871-7","url":null,"abstract":"<div><p>Goldlined seabream (<i>Rhabdosargus sarba</i>), a species with a cosmopolitan distribution, is claimed to be one of the most sought-after and commercially significant fishes which belong to the family Sparidae. This study was conducted to ascertain the significance of spawning aggregation behaviour, in conjunction with the individual spawning performance of <i>R. sarba</i>, by examining five different sex ratio trial combinations and their responses to different hormonal induction dosages. For the induced breeding trials, subadults of <i>R. sarba</i> were stocked and maintained in the cages throughout the year. Fishes were cannulated and found out the initial presence of matured oocytes (468 ± 19 µm) in December and milting males from October to January. Notably, <i>R. sarba</i> was able to mature and induce breed under captivity for the first time in India. Spawning occurred during December, which was administered by a single dose of 500 IU HCG. The fecundity was about 151,410 ± 168 eggs, where the fertilized eggs (889 ± 0.12 µm) with a single oil globule were obtained and the fertilization rate was 75 ± 17% after a latency period of about 36 h. The eggs were incubated and hatched after 14–16 h at a percentage of 79 ± 23%. Further, the larval rearing was carried out until fry size.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitin and its effects when included in aquafeed","authors":"Pascon G.,&nbsp;Opere Akinyi R.,&nbsp;Cardinaletti G.,&nbsp;Daniso E.,&nbsp;Messina M.,&nbsp;Tulli F.","doi":"10.1007/s10499-025-01879-z","DOIUrl":"10.1007/s10499-025-01879-z","url":null,"abstract":"<div><p>Chitin, the second most abundant polysaccharide in nature after cellulose, has been the subject of increasing interest in recent years, particularly in the aquaculture sector. This review focuses on the effects of chitin in aquafeed on growth, nutrient utilization, gut microbiome modulation, and the immune system of aquatic organisms. Data from the literature showed significant variability in response to chitin by species, with some species benefiting from dietary chitin supplementation in terms of growth and immune health. However, excessive chitin inclusion led to negative effects on the protein and lipid digestibility. These results suggest that optimizing the amount of chitin-containing ingredients in aquafeed requires a deep understanding of each species’ nutritional needs and other studies needed for different aquaculture species. Furthermore, studies have highlighted the potential of chitin as an immunostimulant and promoter of gut health. In conclusion, chitin appears to be a promising additive for sustainable aquaculture, but further research is needed to define best practices for its use.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-025-01879-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143446474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the effects of Siraitia grosvenorii fruit on growth performance, immune response, antioxidant gene expression, and resistance to Vibrio parahaemolyticus in Litopenaeus vannamei shrimp","authors":"Roshmon Thomas Mathew,&nbsp;Nashi Khaled Alqahtani,&nbsp;Yousef Ahmed Alkhamis,&nbsp;Rashid Saleh Alngada,&nbsp;Raed Abdul Whed,&nbsp;Nouh Abdulaziz Aljaafari,&nbsp;Lamya Ahmed Alkeridis,&nbsp;El-Sayed Hemdan Eissa,&nbsp;Sameh A. Abdelnour,&nbsp;Moaheda E. H. Eissa,&nbsp;Fatma Mahsoub","doi":"10.1007/s10499-025-01861-9","DOIUrl":"10.1007/s10499-025-01861-9","url":null,"abstract":"<div><p>A long-term feeding study was conducted to evaluate the effects of dietary supplementation with dried <i>Siraitia grosvenorii</i> fruit (SGF) on the growth performance, antioxidant status, hemolymph biochemistry, immune response, and resistance to <i>Vibrio parahaemolyticus</i> of <i>Litopenaeus vannamei</i> shrimp. Two hundred and forty healthy shrimp were randomly assigned to four experimental groups. Shrimp was fed a basal diet containing 0 (SGF0), 300 (SGF300), 450 (SGF450), or 600 (SGF600) mg of <i>Siraitia grosvenorii</i> fruit (SGF) per kilogram of feed for a 90-day feeding trial. The results demonstrated that dietary supplementation with SGF significantly enhanced growth performance and feed utilization (<i>P</i> &lt; 0.05) in shrimp. Significantly higher levels of crude protein and lipid content were observed in the SGF-supplemented groups compared to the control group (<i>P</i> &lt; 0.05). Shrimp in all SGF-supplemented groups exhibited significant enhancements in serum digestive enzyme activities (amylase, lipase, and protease; <i>P</i> &lt; 0.001) and immunoglobulin levels (IgA and IgM; <i>P</i> &lt; 0.001). Moreover, blood protein levels were significantly elevated in the SGF450 and SGF600 groups compared to the control group (<i>P</i> &lt; 0.001). SGF supplementation significantly decreased liver enzyme activities (GGT, AST, LDH, and ALT), with the most pronounced reductions observed at the 450 and 600 mg/kg diet levels. All SGF groups significantly lowered the lipid profile, including total cholesterol (TC), triglycerides (TG), and low-density lipoprotein (LDL) compared to diet-free SGF (<i>P</i> &lt; 0.05). Dietary inclusion of 450 or 600 mg of SGF/kg diet significantly enhanced antioxidant parameters, such as total antioxidant capacity (TAC), glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) compared to SGF300 and control groups (<i>P</i> &lt; 0.05). Conversely, all SGF-supplemented groups exhibited a significant decrease in malondialdehyde (MDA) levels. The expression of the growth hormone (<i>GH</i>) gene and antioxidant-related genes (<i>catalase, cMnSOD</i>, and <i>GPx1</i>) was significantly upregulated following SGF supplementation. The survival rate was higher in shrimp given SGF in their diets. In conclusion, dietary supplementation with SGF enhanced blood health and growth performance in shrimp by stimulating antioxidant gene expression and immune function. These findings suggest the potential of SGF as a promising growth promoter in shrimp aquaculture.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143446475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biometric relationships and condition factor of Nile tilapia (Oreochromis niloticus) grown in concrete ponds with groundwater","authors":"Luis Lorenzo Carrillo La Rosa,&nbsp;Sergio Morell-Monzó,&nbsp;Vicente Puig-Pons,&nbsp;Isabel Pérez-Arjona,&nbsp;Víctor Espinosa","doi":"10.1007/s10499-025-01839-7","DOIUrl":"10.1007/s10499-025-01839-7","url":null,"abstract":"<div><p>This study investigates biometric relationships in <i>Oreochromis niloticus</i> (gray tilapia) reared in controlled pond environments at the Centro de Investigación Piscícola (CINPIS), Universidad Nacional Agraria La Molina (Perú), over a 3-year period (2021–2023). Focusing on total length (<span>({L}_{t})</span>), standard length (<span>({L}_{s})</span>), height (<span>(H)</span>), and width (<span>(A)</span>), we developed models to estimate weight (<span>(W)</span>) based on these parameters, achieving strong model performances with R² values between 0.899 and 0.994. The model using <span>({L}_{t})</span> as a predictor of <span>(W)</span> proved most accurate with a mean relative error (MRE) of 11.2%, while models incorporating additional dimensions (<span>(H)</span> and <span>(A)</span>) did not enhance predictive accuracy. Comparative analyses show our model aligns with some studies on tilapia, though variations in L-W relationships due to environmental and breeding conditions are evident. Our results affirm the utility of <span>({L}_{t})</span> in non-invasive biomass estimation for aquaculture, while highlighting the limitations of applying these models universally across different conditions and fish populations. Condition factor (<span>(K)</span>) and relative condition factor (<span>({K}_{r})</span>) analyses further demonstrated stable and optimal growth conditions (mean <span>(K)</span> ≈ 1.76; <span>({K}_{r})</span> ≈ 1.01) for tilapia under local culture practices. Accordingly, we propose the L-W relationship <span>(W= {0.0265L}^{2.8469})</span> for estimating the weight of gray tilapia grown in ponds with groundwater. This study provides a basis for the development of biomass estimation methods based on active acoustics or stereo video.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-025-01839-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143446473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frozen detritus: a novel nutritious food for juvenile mussels in captive breeding","authors":"Vojtěch Macháček,&nbsp;Ondřej P. Simon,&nbsp;Jitka Horáčková","doi":"10.1007/s10499-025-01873-5","DOIUrl":"10.1007/s10499-025-01873-5","url":null,"abstract":"<div><p>Captive breeding of freshwater mussels is essential for conservation strategies to maintain populations of endangered bivalve mollusks. In the Czech Republic, breeders use detritus from natural sources, preferably from nearby streams and spring areas, to prepare pearl mussels for release into the target localities to reestablish sustainable populations in their native habitats. This strategy is subject to sudden changes, and breeders must consider different detritus qualities between seasons and the unpredictability of weather conditions. Therefore, harvesting natural detritus on a regular basis is challenging and time-consuming, and more samples require more testing, which increases the cost of captive breeding. We proved that juvenile freshwater pearl mussels grew faster when fed detritus stored frozen in a freezer than when fed the same chilled detritus. Therefore, detritus can be harvested under optimal conditions and stored in a freezer for the remaining season, thereby simplifying and reducing mussel conservation costs. Moreover, this approach offers a sustainable, environmentally friendly method that does not require the industrial production of artificial feeds.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-025-01873-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143446550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of the Edwardsiella ictaluri whole-cell vaccine in controlling enteric septicemia of catfish disease in striped catfish (Pangasianodon hypophthalmus)","authors":"Uni Purwaningsih,&nbsp;Sukenda Sukenda,&nbsp;Angela Mariana Lusiastuti,&nbsp;Alimuddin Alimuddin,&nbsp;Widanarni Widanarni,&nbsp;Sri Nuryati","doi":"10.1007/s10499-025-01881-5","DOIUrl":"10.1007/s10499-025-01881-5","url":null,"abstract":"<div><p>In the pangasianodon aquaculture industry at Indonesia, vaccination is considered a backup plan for treating the enteric septicemia of catfish (ESC) diseases. The varied phenotypic and genotypic features of <i>Edwardsiella ictaluri</i> isolates from different aquaculture locations in Indonesia can be used to discover potential vaccine formulations that offer the highest protection against ESC infection. The aim of this work is to establish the best vaccine formulation to prevent ESC infection and to evaluate the synergy and efficacy of multiple antigens in generating immunity in striped catfish. The striped catfish were given 0.1 mL fish⁻¹ of the monovalent, bivalent, and polyvalent-inactivated <i>E. ictaluri</i> bacterin vaccinations intraperitoneally. The assessment of phagocytic activity, lysozyme activity, respiratory burst using the nitro blue tetrazolium (NBT) assay, differential leukocyte count, relative percent survival (RPS), and survival rate (SR) were among the parameters used to evaluate the success of the vaccination. <i>E. ictaluri</i> vaccine preparations from monovalent, bivalent, and polyvalent are inactivated with 0.3% formalin. The monovalent, bivalent, and polyvalent vaccines of <i>E. ictaluri</i> were found to enhance both specific and non-specific immune responses, including increased antibody titers, phagocytic activity, lysozyme production, respiratory burst, and lymphocyte count compared to the control group after 21 days of immune induction and post-challenge test. Our findings indicated that the <i>E. ictaluri</i> polyvalent-inactivated vaccine, which is administered via injection and contains 0.3% formalin, has a greater potential to provide protection than the monovalent and bivalent vaccines. The polyvalent <i>E. ictaluri</i> vaccines were made from the PJbH, P, and PBm1G antigens. Compared to the monovalent, bivalent vaccines, and control, the polyvalent <i>E. ictaluri</i> vaccine, at a dosage of 10¹⁰ CFU fish⁻¹, offered the highest protection significant against multiple <i>E. ictaluri</i> infections, with an RPS value of 61.02%. It could also elicit both specific and non-specific immune responses.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143446544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fecal metabolic variations of Apostichopus japonicus induced by dietary sea mud components in the recirculating aquaculture environment","authors":"Chenyu Song,&nbsp;Ruiguang Dong,&nbsp;Yijing Zhou,&nbsp;Xian Li,&nbsp;Dengpan Dong,&nbsp;Zhitao Huang,&nbsp;Xiefa Song,&nbsp;Guangjun Zhou","doi":"10.1007/s10499-024-01821-9","DOIUrl":"10.1007/s10499-024-01821-9","url":null,"abstract":"<div><p>Sea mud is an important component of diet in <i>Apostichopus japonicus</i> recirculating aquaculture system (RAS), which has a crucial effect on the water environment and metabolic performance of <i>A. japonicus</i>. In this study, we investigated the potential toxic mechanism of sea mud on <i>A. japonicus</i> and interaction mechanism between fecal microorganisms and metabolites in RAS. Thus, this study set up five experimental groups to obtain the optimal amount of sea mud addition to diet. The proportion of sea mud added to diet was 50% (FB1), 67% (FB2), 75% (FB3), 80% (FB4), and 83% (FB5), respectively. The results showed that there was a significant difference in fecal metabolite expression in the comparison group of FB4_vs_FB1 and FB5_vs_FB1 (<i>P</i> ≤ 0.05). A total of 73 potential biomarkers, such as uridine and inosine, were identified by ROC curve analysis that could indicate the toxic effects of sea mud. Topological analysis showed that glycerophospholipid metabolism pathway was upregulated in the comparison group of FB5_vs_FB1 (<i>P</i> ≤ 0.05). However, five pathways including the pyrimidine metabolism pathway, arginine and proline metabolism, purine metabolism, aminoacyl tRNA biosynthesis, and carbapenem biosynthesis pathway were significantly downregulated in group FB5_vs_FB1 (<i>P</i> ≤ 0.05). Glycerophospholipids and pyrimidine pathway in group FB5 was disrupted, leading to immune dysfunction and stress tolerance, which might be the important cause of intestinal inflammation in <i>A. japonicus</i>. Furthermore, a significant correlation between fecal metabolites and microorganisms was found. In brief, our study demonstrated the optimal sea mud addition to diet should be less than 80%, which could provide a basis for the environment protection and optimization of <i>A. japonicus</i> RAS.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-024-01821-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143446551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of dominant Streptococcus iniae strains from diseased rockfish (Sebastes schlegelii) in South Korea: phenotypic, genetic, and pathogenic profiles","authors":"Tae-Ho Kim,&nbsp;Soo-Jin Kim,&nbsp;Mi-Young Cho,&nbsp;Hyun-Ja Han","doi":"10.1007/s10499-025-01868-2","DOIUrl":"10.1007/s10499-025-01868-2","url":null,"abstract":"<div><p><i>Streptococcus iniae</i> is a pathogen that affects fish, causing considerable economic losses in aquaculture worldwide. From 2002 to 2021, an analysis of 57 strains causing streptococcosis in cultured rockfish (<i>Sebastes schlegelii</i>) in South Korea, using 16S rRNA sequencing, revealed that 82.46% of the cases were caused by <i>S. iniae</i>. To enhance our understanding of <i>S. iniae</i> infection, we investigated its phenotypic, genotypic, and pathogenic profiles. Biochemical characteristics analysed using the API 20 strep kit and haemolytic activities (α-haemolysis, 15.6%; β-haemolysis, 81.3%; γ-haemolysis, 3.1%) varied based on the isolation area. Genotypic profiling was performed using multilocus sequence analysis (MLSA) of ten genes: <i>cpsD</i>, <i>mutS</i>, <i>rpoB</i>, <i>glnA</i>, <i>gyrB</i>, <i>prkC</i>, <i>tkt</i>, <i>pheT</i>, <i>arcC</i>, and <i>groEL</i>. Phylogenetic tree identified two clades, with 90.6% of isolates belonging to MLSA-clade 1. Pathogenicity experiments using juvenile rockfish (average weight, 14.94 g) demonstrated that MLSA-clade 1 isolates caused significantly higher mortality than MLSA-clade 2 isolates following intraperitoneal injection. The lethal dose (LD₅₀) for <i>S. iniae</i> isolates 17FBSIn0006, 23FBStr0601, 23FBStr0602, and 23FBStr0603 were 4.58 CFU/fish, 4.28 CFU/fish, 4.71 CFU/fish, and 5.6 × 10⁵ CFU/fish, respectively. Additionally, <i>Lactococcus garvieae</i> 1708–35 (LD₅₀: 1.57 × 10⁷ CFU/fish) and <i>S. parauberis</i> Namhae17 (LD₅₀: 3.19 × 10⁶ CFU/fish) exhibited relatively low pathogenicity in rockfish. Sequence alignment of c<i>psD-</i>encoded capsular polysaccharide D protein of <i>S. iniae</i> isolates revealed differences at three amino acid positions (123, 129, and 159); these were assumed to be related to the pathogenic diversities of <i>S. iniae</i>. Our findings provide valuable insights into streptococcosis in rockfish.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143446545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}