Aquaculture International最新文献

{"title":"Correction to: Would dependency on fossil fuels affect food security and sustainable production in aquaculture? Cultural energy use and energy use efficiency for earthen pond European seabass (Dicentrarchus labrax L., 1758) production","authors":"Gürkan Diken, Hayati Koknaroglu, Ergi Bahrioğlu","doi":"10.1007/s10499-024-01782-z","DOIUrl":"10.1007/s10499-024-01782-z","url":null,"abstract":"","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: The effects of nano‑selenium and/or vitamin C on the growth performance, blood health, organ histology, molecular alterations, and disease resistance of Nile tilapia (Oreochromis niloticus) against Saprolegnia ferax","authors":"Hayat Ali Alafari, Najah M. Albaqami, Yasmin M. Abd El‑Aziz, Yasmin A. Reyad, El‑Sayed Hemdan Eissa, Zulhisyam Abdul Kari, Moaheda E. H. Eissa, Saadiah Ibrahim, Saleha Khan, Mohammad Bodrul Munir, Abdelazeem M. Algammal, Ismail Youssef, Mohammed Ahmed Ali Elshaer, Mohamed Awad Abd‑Elraheem, Sameh A. Abdelnour, Ola Hasan Abd El Megeed","doi":"10.1007/s10499-024-01783-y","DOIUrl":"10.1007/s10499-024-01783-y","url":null,"abstract":"","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142761984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influences of dietary protein source and extrusion temperature on growth performance, tissue composition, and gut microbiota of grass carp Ctenopharynodon idellus","authors":"Zhimin Zhang,&nbsp;Qiushi Yang,&nbsp;Tingting Xu,&nbsp;Haokun Liu,&nbsp;Junyan Jin,&nbsp;Yunxia Yang,&nbsp;Xiaoming Zhu,&nbsp;Dong Han,&nbsp;Shouqi Xie","doi":"10.1007/s10499-024-01768-x","DOIUrl":"10.1007/s10499-024-01768-x","url":null,"abstract":"<div><p>This study aimed to investigate the effects of dietary protein source (DPS) and extrusion temperature (ET) on the physical qualities of extruded grass carp feed and the growth performance, tissue composition, and gut microbiota of these animals. We used a 2 × 2 factorial experimental design, with DPSs consisting of mostly plant or fishmeal proteins extruded at 120 °C (DP1 and DA1 diets) or 140 °C (DP2 and DA2 diets) for grass carp. The growth trial was performed for 9 weeks, and the gut microbiota trial lasted 4 weeks. Compared with the fishmeal-based diets, the plant protein-based diets produced higher expansion ratios (<i>P</i> &lt; 0.001). A high ET also improved the expansion ratio (<i>P</i> &lt; 0.001) and significantly decreased the feed pellet bulk density (<i>P</i> &lt; 0.001). The DP2 diet had the highest hardness and water solubility index and the lowest water absorption index. The DP1 diet exhibited the lowest protein and phosphorus apparent digestibility coefficients (ADCs). The ET significantly affected the ADCs for the plant protein-based diets, but not for the fishmeal-based diets. Grass carp fed the DP1 diet presented the lowest weight gain and specific growth ratio. The interactions between DPS and ET affected the final body weight (<i>P</i> = 0.030), protein efficiency ratio (<i>P</i> = 0.011), and feed efficiency (<i>P</i> = 0.016), and these indices were significantly greater in grass carp fed the DP2 diet than in those fed the DP1 diet. However, DPS and ET did not affect the concentrations of crude protein or amino acids in the dorsal muscle. Compared with grass carp fed plant-based diets, those fed fishmeal-based diets presented greater fat contents in the muscle (<i>P</i> = 0.011) and liver <i>P</i> = 0.037), and a greater hepatosomatic index (<i>P</i> = 0.003). The interaction of DPS and ET influenced the viscerosomatic index (VSI, <i>P</i> = 0.001), with the highest VSI found after feeding the DA2 diet. DPS (<i>P</i> = 0.001) but not ET (<i>P</i> = 0.172) significantly affected the gut microbial communities, although the plant protein-based diets with increased ET resulted in more differential taxa and greater differences in the abundances of the core microbiota in grass carp compared to the fishmeal-based diets. These results indicate that improving the ET of plant protein-based diets can alter the physical qualities of the diets, further affecting the gut microbiota, and enhancing the growth performance of grass carp. These findings reveal that higher ETs are needed for plant protein-based diets than for fishmeal-based diets, which is a valuable guideline for feed production.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-024-01768-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-parasitic efficacy of green-synthesized silver nanoparticles on Argulus siamensis: An ectoparasite of fish and their effect on the expression of ion channel genes","authors":"Pushpa Kumari,&nbsp;Saurav Kumar,&nbsp;Rajive Kumar Brahmchari,&nbsp;Angom Baleshwor Singh,&nbsp;Kooloth Valappil Rajendran,&nbsp;Satya Prakash Shukla,&nbsp;Rupam Sharma,&nbsp;Ram Prakash Raman","doi":"10.1007/s10499-024-01675-1","DOIUrl":"10.1007/s10499-024-01675-1","url":null,"abstract":"<p>Argulosis, a serious fish disease caused by the crustacean ectoparasite <i>Argulus</i>, results in considerable losses to the global aquaculture industry. This study evaluated the in vitro anti-parasitic efficacy (AE%) of synthesized silver nanoparticles (AgNPs) using an aqueous leaf extract of <i>Azadirachta indica</i> (neem) against the adult and copepodid stages of <i>Argulus siamensis</i>. The biosynthesized AgNPs were characterized using UV–Vis spectroscopy, which revealed a surface plasmon resonance peak at 425 nm, with a zeta potential value of − 28.5 mV. TEM analysis verified that the nanoparticles were ranging from 5 to 50 nm in size, spherical in shape, and capped by various phytochemicals identified through Fourier-transform infrared spectroscopy (FTIR). Treatment with AgNPs showed an <i>AE</i> of 100% against copepodids at 25 ppm and adult <i>Argulus</i> at 50 ppm, with an estimated 6-h <i>EC</i>₅₀ of 9.66 and 16.03 ppm, respectively. No mortality was found in the control, leaf extract, and AgNO₃-treated groups of parasites. Additionally, gene expression studies using β-actin as a reference gene revealed significantly upregulated levels of all tested genes, with the highest fold change in the GABA gene across all treatment groups. The increased <i>AE</i> of AgNPs may be due to altered signal transduction, as evidenced by the significantly increased expression of ion channel genes compared to control and neem leaf extract-treated groups. The findings of this study indicate the potential for developing a promising nanodrug for the safe and effective control of argulosis in aquaculture systems.</p>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of correlation between daily rhythm of digestion and metabolism of grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀) and water quality parameters in recycling aquaculture system","authors":"Wenyang Li,&nbsp;Hongxu Li,&nbsp;Fan Fei,&nbsp;Xiaoqiang Gao,&nbsp;Shuquan Cao,&nbsp;Wensheng Li,&nbsp;Bo Jin,&nbsp;Baoliang Liu","doi":"10.1007/s10499-024-01706-x","DOIUrl":"10.1007/s10499-024-01706-x","url":null,"abstract":"<div><p>The daily rhythm is a critical regulatory factor in fish growth and development, governing internal functions and organismal responses to environmental cues. This rhythm influences not only metabolism but also water quality parameters in recirculating aquaculture systems (RAS). However, research on the daily rhythm of hybrid groupers in RAS remains limited. Consequently, this study is aimed at investigating the metabolic daily rhythm of hybrid groupers in RAS and its impact on water quality. Feeding time was designated as the starting point for this investigation. Initially, we measured the levels of dissolved oxygen (DO), total ammonia nitrogen (TAN), and nitrite nitrogen (Nitrite-N) in the RAS. Subsequently, we assessed oxygen consumption rates (OCR), digestive enzyme activities, and the expression levels of metabolism-related genes in the pearl gentian grouper (<i>Epinephelus lanceolatus</i>♂ × <i>Epinephelus fuscoguttatus</i>♀). Finally, we conducted a comprehensive analysis of these factors. The results indicated that DO was negatively correlated with OCR, pepsin and lipase activities, and GK gene expression. TAN exhibited a positive correlation with OCR and the expression levels of the target of rapamycin (TOR) gene. Similarly, Nitrite-N showed a positive correlation with OCR and TOR gene expression levels. We also speculated on the internal relationship between fish daily rhythms and water quality parameters, noting a certain degree of synchronicity. These findings can minimize stress on fish, and it can contribute to optimizing RAS performance through management adjustments.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142691954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the relative bioavailability between betaine and l-carnitine for Nile tilapia","authors":"Milena Souza dos Santos Sanchez,&nbsp;Mariana Lins Rodrigues,&nbsp;Jhonis Ernzen Pessini,&nbsp;Fábio Bittencourt,&nbsp;Wilson Rogério Boscolo,&nbsp;Altevir Signor","doi":"10.1007/s10499-024-01771-2","DOIUrl":"10.1007/s10499-024-01771-2","url":null,"abstract":"<p>This study evaluated tilapia fed diets supplemented with levels of betaine and <span>l</span>-carnitine, simultaneously. Diets supplemented with levels of <span>l</span>-carnitine (500 and 1000 mg kg⁻¹ of feed) and betaine (500 and 1000 mg kg⁻¹ of feed) were used, in addition to the control diet. Weight gain, daily weight gain, and specific growth rate in fish fed diets containing 1000–500 and 1000–1000 mg kg⁻¹ betaine and <span>l</span>-carnitine performed worse than the other inclusion levels, including the control diet. The lipid content of fish fillets was higher in those fed with levels of 1000–1000 mg kg⁻¹ of betaine and <span>l</span>-carnitine in the diet. There was a reduction in total plasma protein levels in fish fed with 1000–500 mg kg⁻¹ of betaine and <span>l</span>-carnitine in the diets, and glucose was higher when fish were fed with levels of 1000–1000 mg kg⁻¹ of betaine and <span>l</span>-carnitine in feed. Catalase activity was higher at levels of 1000–500 mg kg⁻¹ of betaine and <span>l</span>-carnitine in the diet. The height and width of the villi had greater development in fish fed with 1000–500 mg kg⁻¹ of betaine and <span>l</span>-carnitine in the diet. Assessment of muscle histology indicated the interaction of betaine and <span>l</span>-carnitine at levels of 1000–500 mg kg⁻¹ of inclusion in the diet, triggering greater muscle growth. The inclusion of 500–500 mg kg⁻¹ of betaine and <span>l</span>-carnitine in diets improves the development of fish, providing a reduction in carcass lipids and greater muscle development, and reducing oxidative stress in the liver.\u0000</p>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary lipid and protein levels on growth, body composition, antioxidant capacity, and flesh quality of mandarin fish (Siniperca chuatsi)","authors":"Shengchao Liu,&nbsp;Shaojie Liu,&nbsp;Zeqiang Sun,&nbsp;Zishuo Fang,&nbsp;Ye Gong,&nbsp;Xuxiong Huang,&nbsp;Haitao Zhang,&nbsp;Naisong Chen,&nbsp;Songlin Li","doi":"10.1007/s10499-024-01716-9","DOIUrl":"10.1007/s10499-024-01716-9","url":null,"abstract":"<div><p>The present study explored the effects of dietary lipid and protein levels on growth performance, body composition, antioxidant capacity, and flesh quality of mandarin fish (<i>Siniperca chuatsi</i>). Nine experimental diets containing graded levels of crude lipid (8%, 12%, and 16%) and crude protein (45%, 50%, and 55%) were formulated in a 3 × 3 factorial design. Eight hundred and ten mandarin fish (mean initial body weight, 20.90 ± 0.02 g) were randomly assigned into 27 tanks (1000 L) and triplicate tanks of fish were fed one of the experimental diets twice daily for 56 days. The results showed that a 16% dietary lipid level could significantly increase the survival rate of cultured fish, while a 55% dietary protein level could significantly increase the final body weight and specific growth rate of mandarin fish. Additionally, there was a significant interaction between dietary lipid and protein levels in terms of feed intake and protein digestibility. Further, the whole body and liver lipid content of cultured fish significantly increased with the increase of dietary lipid level, while the provision of dietary protein level significantly decreased the liver crude lipid content, and there was a significant interaction between dietary lipid and protein levels on the moisture, protein, and lipid content of the liver of cultured fish. In terms of hepatic antioxidant capacity, the total anti-oxidative capacity and the activities of catalase and superoxide dismutase decreased significantly with increasing dietary lipid or protein content. As to flesh quality, muscle hardness, chewiness, gumminess, and shear increased significantly with decreasing muscle lipid content, which is significantly influenced by dietary lipid content. In conclusion, considering all the results, the appropriate levels of lipids and protein in the diet for mandarin fish were 12% and 50%, respectively. This study provided reliable experimental data and theoretical basis for both subsequent research and the application of artificial compound feed for mandarin fish.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of dietary protein and lipid levels and growth rates on the intestinal microbiota of kuruma shrimp (Penaeus japonicus)","authors":"Chuanxi Chen,&nbsp;Chunxiang Ai,&nbsp;Wenzhi Cheng,&nbsp;Huiyang Huang,&nbsp;Guangjie Fang,&nbsp;Yiling Hou,&nbsp;Xiaojie Deng,&nbsp;Siqi Li,&nbsp;Tingting Huang,&nbsp;Yougen Chen,&nbsp;Peng Xu,&nbsp;Yong Mao","doi":"10.1007/s10499-024-01773-0","DOIUrl":"10.1007/s10499-024-01773-0","url":null,"abstract":"<div><p>Gut microbiota is essential for host development and growth, with strong links to diet and growth performance in aquatic species. This study explores the bacterial communities in kuruma shrimp (<i>Penaeus japonicus</i>) under different diets and growth rates. Shrimp with an average initial weight of 0.30 ± 0.08 g were fed diets with varying protein (50%, 48%, and 46%) and lipid (11% and 7% in a 3 × 2 experimental design) for 7 weeks. Shrimp fed a 50% protein and 11% lipid diet exhibited superior growth performance (<i>P</i> &lt; 0.05). At the phylum level, Proteobacteria, Bacteroidota, Firmicutes, and Actinobacteriota were predominant, with the Firmicutes/Bacteroidota ratio increasing in response to dietary protein and lipid levels—a trend also observed in fast-growing shrimp. At the genus level, <i>Vibrio</i>, <i>Ralstonia</i>, <i>Shimia</i>, and <i>Spongiimonas</i> were prevalent, with <i>Shimia</i> identified as a biomarker in shrimp fed a high-lipid diet or those exhibiting rapid growth under identical conditions. Whether in different dietary groups or under the same condition, fast-growing shrimp displayed a more intricate bacterial network, with greater connectivity and a higher diversity of keystone taxa compared to slow-growing shrimp. The intestinal metabolism in groups fed diets with high protein and lipid levels was more active than in those receiving lower levels. Collectively, these results indicate the gut microbiota structure of shrimp with different growth performances shows different characteristics, and dietary adjustments can be used to modulate the gut microbiota structure of <i>P. japonicus</i>, promoting optimal growth performance. The diet containing 50% protein and 11% lipid could be considered optimal for its growth and gut health.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142691995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of selenium on the growth and immune gene functions of red swamp crayfish (Procambarus clakii)","authors":"Sahr Lamin Sumana,&nbsp;Fan Yu,&nbsp;Chen Ming,&nbsp;Zhu Minli,&nbsp;Peipei Wang,&nbsp;Cailin Huang,&nbsp;Abdullateef Mukhtar Muhammad,&nbsp;Falayi Opeoluwa Chrstiana,&nbsp;Shengyan Su,&nbsp;Yu Liao,&nbsp;Zhixin Zhang,&nbsp;Shouxiong Cao","doi":"10.1007/s10499-024-01683-1","DOIUrl":"10.1007/s10499-024-01683-1","url":null,"abstract":"<div><p>Selenium is a widely used essential micronutrients in aquaculture, but its excessive effects on crayfish growth parameters and immune system are still unclear. This study was aimed to investigate the effects of selenium concentration in the soil, muscle, fatty acid profile, and gene expression of crayfish cultured in rice co-culture (CRC) model fed with commercial feed for 3 months in three locations of Guangxi Province. For this purpose, the soil samples of the three locations were collected and analyzed with the muscles of crayfish harvested were analyzed for selenium concentration. The Agilent Technology (7800 ICP-MS) was used to analyze selenium levels in soil, as well as muscle and fatty acid profiles. Additionally, gene expression profiling was conducted using qPCR, along with transcriptome analysis. On the one hand, we observed increased selenium concentrations in soil and muscle samples with significant differences (<i>P</i> &lt; 0.05). Significant increases in AKP and T-AOC were observed in Guiping (<i>P</i> &lt; 0.05), with Gangnan exhibiting the lowest T-AOC levels and Qintang the lowest AKP levels. On the other hand, the boosted antioxidant defenses (SOD and CAT) and reduced lipid peroxidation (MDA) in Gangnan might indicate a more favorable oxidative environment in this area. The results demonstrated that selenium exerted a targeted effect on specific growth-related genes while overall growth remained largely unaffected. Furthermore, it was observed that selenium had a positive effect on the expression of immune-related genes in crayfish, which may have resulted in an enhanced immune response. In conclusion, the notable elevation in selenium levels in the soil, coupled with pronounced discrepancies(<i>P</i> &lt; 0.05), may exert considerable influence on the muscle and fatty acid profile of crayfish. Furthermore, this increase in selenium levels may also have implications for the growth and immune functions of crayfish.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142679895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of high vp28 expression in Synechocystis sp. PCC6803 constructed by homologous recombination on immunity and antioxidant capacity of white spot syndrome virus infected Litopenaeus vannamei","authors":"Ning Ding,&nbsp;Jia Yang,&nbsp;Hang Zhang,&nbsp;Xianghong Qi,&nbsp;Fangyuan Liu,&nbsp;Yifei Shen,&nbsp;Rui Jia","doi":"10.1007/s10499-024-01726-7","DOIUrl":"10.1007/s10499-024-01726-7","url":null,"abstract":"<div><p>This study represents the first instance of integrating the vp28 gene from the white spot syndrome virus (WSSV) into the genome of <i>Synechocystis</i> sp. PCC6803 using homologous recombination technology, resulting in the construction of the homologous recombinant algae strain Hr<i>vp</i>28-6803. Compared to the directly introduced plasmid transgenic cyanobacterium <i>vp</i>28-6803, the expression efficiency of <i>vp</i>28 in Hr<i>vp</i>28-6803 was found to be 2.87 times higher. <i>Litopenaeus vannamei</i> were orally immunized with the transgenic cyanobacteria and subsequently challenged with WSSV 7 days later. The cumulative survival rate of <i>L. vannamei</i> infected with WSSV was 56.66% after 10 days, reflecting a 13.33% increase compared to the <i>vp</i>28-6803 group. After the challenge, immune-related enzyme activities, including alkaline phosphatase (AKP) and acid phosphatase (ACP), as well as antioxidant-related enzyme activities of lactate dehydrogenase (LDH) and total antioxidant capacity (T-AOC), were measured in the hepatopancreas of the shrimp. The enzyme activities of AKP, ACP, and T-AOC in the Hr<i>vp</i>28-6803 group were significantly higher than those in the positive control group and the <i>vp</i>28-6803 group, while the LDH level was significantly lower than that in both the positive control group and the <i>vp</i>28-6803 group. The results demonstrate that the Hr<i>vp</i>28-6803 strain significantly enhances the survival rate of <i>L. vannamei</i> and enhances their immunity and antioxidant capacity. Therefore, the homologous recombinant <i>Synechocystis</i> Hr<i>vp</i>28-6803 can be utilized as an oral immunostimulant against WSSV to provide protection for <i>L. vannamei</i>.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142679786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}