Aquaculture International最新文献

{"title":"Curcumin supplementation efficiently improves shrimp disease resistance via potentiated ecological, mechanical, and immune barriers","authors":"Haonan Sha,&nbsp;Jiayue Wang,&nbsp;Zheliang Sheng,&nbsp;Changyue Zhu,&nbsp;Jinbo Xiong","doi":"10.1007/s10499-026-02630-y","DOIUrl":"10.1007/s10499-026-02630-y","url":null,"abstract":"<div><p>Numerous studies have demonstrated a strong association between dysbiosis in the gut microbiota and disease onset. Curcumin, a polyphenolic bioactive compound extracted from <i>Curcuma longa</i>, exhibits potent anti-inflammatory properties and effectively modulates the gut microbiota in vivo, positioning it as a promising intervention for improving host health. However, the underlying mechanisms remain incompletely understood. In this study, shrimp (<i>Litopenaeus vannamei</i>) were fed the diet with 0.5% curcumin for 14 days and subsequently challenged with a <i>Vibrio</i> cocktail (<i>V. fluvialis</i>, <i>V. coralliilyticus</i>, and <i>V. tubiashii</i>) for 9 days. To investigate the mechanisms by which curcumin supplementation enhances shrimp disease resistance, gut and hepatopancreas samples were collected for analyzing the gut microbiome and shrimp transcriptomic profile, respectively. Compared with the <i>Vibrio</i> spp. infection group, curcumin supplementation plus infection effectively alleviated shrimp mortality (<i>P</i> = 0.006) and hepatopancreatic and intestinal damages, including the deformation of hepatopancreatic tubules, irregular luminal atrophy, and intestinal epithelial necrosis. Infection alone significantly depressed the gut microbial diversity, beneficial bacterial taxa, network stability, and butanoate metabolism compared with controls, while these detrimental effects were alleviated by curcumin supplementation, as supported by the restoration of gut histomorphology, ecological, and mechanical barriers against pathogens. Transcriptome analysis revealed that infection alone significantly suppressed shrimp key innate immune pathways of Toll-like receptor, regulation of actin cytoskeleton, and Toll and Imd signaling pathways compared with controls. However, these immune barriers were markedly activated by curcumin supplementation plus infection shrimp, which could facilitate rapid pathogen recognition and preserve the hepatopancreas structural integrity. Collectively, curcumin supplementation protected shrimp against infection by strengthening ecological, mechanical, and immune barriers. Our findings elucidate the ecological and molecular mechanisms by which curcumin strengthen host disease resistance, providing solid foundation for its application in aquaculture.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148614264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple-stressor responses of Neopyropia yezoensis seedlings to marine heatwaves, nitrogen availability and interspecific competition","authors":"Ruitong Jiang,&nbsp;Chuchu Wang,&nbsp;He Li,&nbsp;Sufang Li,&nbsp;Xiucheng Shang,&nbsp;Hailong Wu","doi":"10.1007/s10499-026-02626-8","DOIUrl":"10.1007/s10499-026-02626-8","url":null,"abstract":"<div><p>Under global climate change, marine heatwaves (MHWs) together with nutrient stress pose increasing threats to macroalgae. Here, the economically important seaweed <i>Neopyropia yezoensis</i> was used to examine the integrated effects of two marine heatwave scenarios (MHW-1: gradual temperature fluctuation; MHW-2: abrupt warming followed by cooling), contrasting nitrogen concentrations, and interspecific competition on the photosynthetic physiology and monospore release of conchospore and monospore seedlings. The results showed that high nitrogen effectively alleviated the inhibitory effects of heatwaves on both seedling types and promoted the synthesis of photosynthetic pigments and phycobiliproteins, whereas high-temperature heatwaves (particularly MHW-2 under low nitrogen) caused the strongest growth inhibition, with the relative growth rate of conchospore seedlings declining by up to 91.05%, and induced pronounced physiological suppression in <i>N. yezoensis</i> seedlings.. Under competitive conditions, conchospore seedlings exhibited stronger adaptability, while monospore seedlings were generally inhibited by competition. Regarding monospore release, release from conchospore seedlings was strongly stimulated by the combined effects of competition and high nitrogen, whereas release from monospore seedlings was mainly regulated by nitrogen availability: under monoculture, low nitrogen favored monospore release, while the stimulatory effect of mixed culture occurred only at early stages and weakened over time. This study systematically reveals how environmental stress and biotic competition jointly drive physiological and reproductive responses in <i>N. yezoensis</i>, providing insights of significance for maintaining nearshore ecosystem stability and enhancing the sustainability of marine aquaculture.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148614365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progressive multimodal interaction network for reliable quantification of fish feeding intensity in aquaculture","authors":"Shulong Zhang,&nbsp;Mingyuan Yao,&nbsp;Jiayin Zhao,&nbsp;Daoliang Li,&nbsp;Yingyi Chen,&nbsp;Haihua Wang","doi":"10.1007/s10499-026-02624-w","DOIUrl":"10.1007/s10499-026-02624-w","url":null,"abstract":"<div><p>Accurate quantification of fish feeding intensity is crucial for precision feeding in aquaculture because it directly affects feed utilization and farming efficiency. Although multimodal fusion has proven effective for this task, existing methods often overlook response inconsistency and decision conflicts across modalities, thereby limiting the reliability of quantification results. To address this issue, this paper proposes a progressive multimodal interaction network (PMIN) that integrates image, audio, and water-wave data for fish feeding intensity quantification. Specifically, a unified feature extraction framework is constructed to map inputs from different modalities into a structurally consistent feature space, thereby reducing representational discrepancies among modalities. An auxiliary-modality reinforcement primary-modality mechanism is further designed to promote cross-modal information fusion through channel-aware recalibration and dual-stage attention interaction. Furthermore, a decision fusion strategy based on adaptive evidence reasoning is introduced to jointly model the confidence, reliability, and conflicts of modality-specific outputs, thereby improving the stability and robustness of the final decision. Experiments were conducted on a multimodal fish feeding intensity dataset containing 7089 samples. The results demonstrated that PMIN achieved an accuracy of 96.76% while maintaining low computational complexity and model size, and its overall performance surpassed that of both homogeneous and heterogeneous comparison models. Ablation studies, comparative experiments, and real-world application results further validated the effectiveness and superiority of the proposed method. The proposed method can provide reliable support for automated feeding monitoring and precise feeding decision-making in intensive aquaculture systems.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148613679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial characterization of a disease event in larval rearing system of tropical rock lobster (Panulirus ornatus)","authors":"Mengjia Jiang,&nbsp;Andrew J. Trotter,&nbsp;Ivona Mladineo,&nbsp;Evan F. Goulden,&nbsp;Gregory G. Smith","doi":"10.1007/s10499-026-02622-y","DOIUrl":"10.1007/s10499-026-02622-y","url":null,"abstract":"<div><p>Disease outbreaks in commercial larval rearing systems of the tropical rock lobster (<i>Panulirus ornatus</i>) pose significant challenges to the culture of this species. This study investigates bacterial succession during outbreaks of white leg disease (WLD), the latter associated with infections by <i>Aquimarina</i> spp. in early-stage <i>P. ornatus</i> phyllosoma. Bacteriome profiling was conducted to assess changes in bacterial composition and identify potential sources of pathogenic taxa. Transmission electron microscopy was used to depict ultrastructural changes in the pereiopods of diseased specimens. Oxford Nanopore 16S rRNA gene sequencing was used to profile bacterial diversity and community structure across larval development (0–16 days post-hatch, dph), revealing temporal shifts coinciding with the onset of disease. A marked decline in alpha diversity between 8 and 16 dph was observed concomitant with an increase in diseased animals. During this period, the pathogenic genus <i>Aquimarina</i> became dominant, replacing early taxa such as <i>Vibrio</i>, <i>Ruegeria</i>, <i>Shimia</i>, and <i>Phaeobacter</i>, and concurring with massive coagulative necrosis and the subsequent formation of ellipsoid, electron-dense, proteinaceous bodies in pereiopod tissues. Beta diversity analysis further revealed significant shifts in community structure, implying that bacterial dysbiosis may be an early indicator of WLD development. The findings suggest that disease results from a combination of bacterial maternal or environmental transmission, environmental acquisition, and bacteria-bacteria interactions. The study highlights the importance of maintaining bacterial balance to support a healthy aquaculture system for larval lobsters, suggesting that early bacterial disruption can facilitate disease emergence.\u0000</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-026-02622-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148614071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary wheat bran pretreated with xylanase and glucanase mixture modulates growth performance, antioxidant capacity, and immune response in Nile tilapia fingerlings","authors":"Benjamin U. Akpoilih,&nbsp;Ibrahim Adeshina,&nbsp;Adibabari T. Poromon,&nbsp;Mohsen Abdel-Tawwab","doi":"10.1007/s10499-026-02615-x","DOIUrl":"10.1007/s10499-026-02615-x","url":null,"abstract":"<div><p>This study was carried out to evaluate the use of wheat bran (WB), pre-treated with exogenous xylanase (X) and glucanase (G) mixture, as an energy source for Nile tilapia (<i>Oreochromis niloticus</i>). However, WB was pre-treated with different concentrations of X and G (X + G) blend (0.0 (control), 0.1, 0.2, 0.3, and 0.4 g/kg WB), for 8 h. Pre-treated WB was incorporated into five isocaloric (18.66 kJ/g gross energy) and isonitrogenous (30% crude protein) diets, designated as WB_0.0, WB_0.1, WB_0.2, WB_0.3, and WB_0.4, respectively. The diets were fed to triplicate groups of Nile tilapia (<i>Oreochromis niloticus</i>) fingerling (8–9 g/fish) stocked into 100-L tanks, at 10 fish per tank, three times a day, for 56 days. Compared with the control group (WB_0.0), fish fed on WB-pretreated diets (WB_0.2, WB_0.3, and WB_0.4) showed significant (<i>p</i> &lt; 0.05) increases in zootechnical performance, with no discernible differences (<i>p</i> &gt; 0.05) among enzyme-treated groups. Similarly, fish fed on WB_0.2, WB_0.3, and WB_0.4 diets consumed more feed than those fed the control diet (WB_0.0), although FCR values did not change significantly (<i>p</i> &gt; 0.05). Intestinal protease and lipase activities were considerably higher (<i>p</i> &lt; 0.05) in fish fed on WB_0.3 and WB_0.4 diets than in the control group (WB0.0). Fish fed on WB_0.3 and WB_0.4 diets showed a significant reduction (<i>p</i> &lt; 0.05) in blood aspartate and alanine aminotransferases, urea nitrogen, and creatinine levels, with an increase in red blood cell and white blood cell counts, total protein, albumin, and globulin compared to other groups. Furthermore, fish given WB_0.3 and WB_0.4 diets showed higher levels of antioxidant and immunological bioindicators than the control group (WB_0.0). The results of this study recommend the pretreatment of WB with 0.25–0.30 g of xylanase and glucanase blend per kg of WB before its inclusion n fish diets. Feeding Nile tilapia fingerlings on this WB pretreatment level could enhance the growth performance, antioxidant, and immunological biomarkers.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148613619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inclusion of carotenoids and Eicosapentaenoic Acid (EPA) in sea urchin (Paracentrotus lividus) broodstock diets improves gonad development and oocyte quality","authors":"A. S. Gomes,&nbsp;A. F. Rodrigues,&nbsp;M. Neves,&nbsp;C. Roseiro,&nbsp;H. Cardoso,&nbsp;A. P. Violante,&nbsp;P. J. Gavaia,&nbsp;C. Suckling,&nbsp;S. Lourenço","doi":"10.1007/s10499-026-02609-9","DOIUrl":"10.1007/s10499-026-02609-9","url":null,"abstract":"<div><p>In aquaculture, broodstock conditioning diets are essential for enhancing reproductive performance, particularly as offspring survival remains a primary bottleneck in sea urchin production. This study investigated the effects of different broodstock diets on gonad development, fatty acid profiles, and oocyte size in the sea urchin <i>Paracentrotus lividus</i>. To determine if maternal investment could be enhanced through specific nutrients, three diets that differed in both ingredient matrix and pigment profile were tested over a two-month feeding period: 1) a formulated pumpkin/algal diet (PA) enriched with β-carotene via pumpkin and <i>Nannochloropsis</i> sp.; 2) a formulated pea/corn diet (PC) with a low-pigment profile, and 3) a natural kelp diet based on <i>Saccorhiza polyschides,</i> representing one of the natural foods for <i>P. lividus</i> in its local habitat. This Kelp diet provided a mixed-pigment reference with relatively high total carotenoids. Following the feeding trial, several key parameters were assessed, including gonadosomatic index (GSI), reproductive stage, fatty acid composition, oocyte size, and fertilization success. Results indicated that females fed the PA diet produced oocytes that were 18% larger and achieved a 7% higher fertilization rate than those on the PC diet. This enhancement is likely linked to the significantly higher deposition of carotenoids and omega-3 polyunsaturated fatty acids (n-3 PUFA) in the gonads. In contrast, while the PC diet promoted a high GSI, it resulted in smaller oocytes and lower fertilization rates, due to a deficiency in n-3 PUFA in their gonads. Females fed the Kelp diet presented the slowest gonadal development and failed to spawn, suggesting that seaweed is insufficient as a sole food source for short-term broodstock conditioning to support reproduction. This research provides valuable insights into developing broodstock conditioning diets by exploring alternative nutrient sources, such as pumpkin and microalgae, to enhance reproductive traits, particularly for emerging species like sea urchins.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148614072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of transcriptional biomarkers for ammonia stress in fish","authors":"Anna Duenser,&nbsp;Ehsan Pashay Ahi","doi":"10.1007/s10499-026-02618-8","DOIUrl":"10.1007/s10499-026-02618-8","url":null,"abstract":"<div><p>Ammonia loading is recognized as a pervasive constraint in intensive aquaculture and eutrophic waters, yet transcriptional indicators of ammonia stress have been reported across disparate species, tissues, and exposure regimes. In this review, fish studies linking ammonia exposure to mRNA responses are synthesized using a tissue-forward framework that prioritizes first-contact epithelia and downstream metabolic organs. In gills, recurring modulation is described for Rhesus glycoproteins, particularly <i>Rhbg</i> and <i>Rhcg</i>/<i>RhCG</i> isoforms, and associated acid–base and ionoregulatory machinery (V-type H + -ATPase, Na + /H + exchangers, carbonic anhydrase, Na + /K + -ATPase), alongside inflammatory and remodeling signals consistent with epithelial disturbance. In liver and other internal tissues, coherent shifts are reported in amino-acid and nitrogen metabolism, mitochondrial energy pathways, redox control, and cell-death programs, with representative candidate biomarkers including glutamine synthetase, carbamoyl phosphate synthetase I, antioxidant/redox genes, cytokine-related markers, and apoptosis/ferroptosis-associated transcripts. Because many oxidative and immune transcripts are shared among stressors, improved diagnostic value is expected when biomarker panels are derived from co-regulated modules using network-guided reduction approaches, including weighted gene co-expression network analysis (WGCNA), and validated against key confounders. Candidate gene sets are collated and a roadmap is outlined for translating tissue signatures into compact assays for aquaculture management and field surveillance.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-026-02618-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148613680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of floc meal and animal waste derived proteins on growth performance and metabolic responses of juvenile Anabas testudineus during winter rearing","authors":"Md Aklakur,&nbsp;Md Imran Shah,&nbsp;Md Amjad,&nbsp;Udipta Roy,&nbsp;Potluri Sai Kishore,&nbsp;Sangeeta Mandal,&nbsp;Barkha Asthana","doi":"10.1007/s10499-026-02613-z","DOIUrl":"10.1007/s10499-026-02613-z","url":null,"abstract":"<div><p>An experiment was conducted to evaluate the effects of various animal wastes as minor ingredients in growth, feed utilisation, and overall health status of <i>Anabas testudineus</i> in winter<i>.</i> In winter, fish productivity declines rapidly due to reduced metabolic activity, declined natural productivity, and stress. Utilisation of selected animal wastes may enhance aquaculture productivity by enhancing feed attractability and intake due to their free amino acids, soluble peptides, and flavour compounds that stimulate olfactory and gustatory response in fishes. The waste products: fish waste digest (FWD), biofloc meal (BFM), chicken waste hydrolysate (CWH), shrimp head meal (SHM), and beef liver meal (BLM) were supplemented at 5% inclusion rate in iso-nitrogenous and iso-lipid diet formulation and compared with control feeds. The fishes with initial weight of 1.5–1.6 g were stocked at 400/m³ density and cultured for 90 days. Throughout the experiment, the temperature ranged between 10.3 and 18.1 °C and all other water quality parameters were maintained at appropriate level. The growth parameters indicated significantly higher (<i>p</i> &lt; 0.05) percentage weight gain (361.45 ± 3.50%), specific growth rate (0.66 ± 0.01%), average daily growth (1.24 ± 0.01 g), survivability (73.33 ± 6.01%), and improved feed conversion ratio (1.25 ± 0.01) in the BFM fed group. Similarly, the haemato-biochemical parameters indicated significantly higher haemoglobin (10.67 ± 0.26 g/dl), haematocrit (4.80 ± 0.15%), total erythrocyte count (34.83 ± 0.09) × 10⁶ cells/mm³, total protein (3.63 ± 0.09 g/dl), albumin (0.94 ± 0.03 g/dl), globulin (2.69 ± 0.10 g/dl), and A/G ratio (0.35 ± 0.02) in BFM group. Inflammatory markers, digestive enzymes, and antioxidant enzyme levels demonstrated improved physiological responses in BFM group compared with control and other supplemented groups, supporting overall growth and haematological parameters of experimental animals. Whole body proximate indices suggest significantly higher lipid and ash content in control group (C) compared to other supplemented groups and however, crude protein content in BFM group compared with other supplemented groups. Therefore, supplementation of BFM at 5% inclusion rate enhances overall physiological welfare and survivability of <i>Anabas testudineus</i> cultured during winter.\u0000</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148613800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental performance of near-zero discharge recirculating aquaculture systems: an exergoenvironmental assessment","authors":"Haydar Küçük,&nbsp;Uğur Akbulut,&nbsp;İlhan Aydın","doi":"10.1007/s10499-026-02620-0","DOIUrl":"10.1007/s10499-026-02620-0","url":null,"abstract":"<div><p>Near-zero discharge recirculating aquaculture systems (RAS) are increasingly promoted as biosecure and water-efficient technologies that reduce local environmental impacts. However, their high energy consumption can significantly affect the overall environmental footprint. This research presents a comprehensive exergoenvironmental analysis of a commercial-scale, near-zero discharge RAS focused on turbot (<i>Scophthalmus maximus</i>) production. The study aims to quantify environmental impacts at both the component and stream levels by integrating life cycle assessment (LCA) with the specific exergy costing (SPECO) methodology. The operational phase was found to be the primary contributor to the environmental impact, accounting for 98.9% of the total burden, whereas capital investment accounted for only 1.1%. Component-level analysis identified the circulation pump as the largest hotspot, with a total impact rate of 634.5 mPts/h, followed by the chiller unit at 420.5 mPts/h and the oxygen generator at 417.5 mPts/h. Stream-level assessment revealed that the specific environmental impact of the main circulated water stream increased by 56% throughout the cycle, rising from 182.3 mPts/kWh at the tank outlet to 285.8 mPts/kWh at the tank inlet. The total environmental impact associated with the final product was determined to be 1227.4 mPts per kilogram of harvested turbot. Sensitivity analysis demonstrated that transitioning to renewable electricity sources could reduce the environmental footprint by 64.3%, reducing it to 438.2 mPts/kg. These findings indicate that improvements in the environmental performance of near-zero discharge RAS should prioritize operational energy efficiency and electricity decarbonization rather than material substitution strategies.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148613233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crab tracking and activity estimation method based on YOLOv11-BoT-SORT","authors":"Fu Jiayi,&nbsp;Kong Mingrui,&nbsp;Pan Hongyu,&nbsp;Li Chenlong,&nbsp;Duan Qingling","doi":"10.1007/s10499-026-02614-y","DOIUrl":"10.1007/s10499-026-02614-y","url":null,"abstract":"<div><p>The Chinese mitten crab is one of China’s main aquaculture species. Tracking and calculating activity levels can reflect its health status and behavioral characteristics, aiding in improving farming efficiency. However, due to the high similarity in dorsal shell appearance, tracking algorithms face issues such as low accuracy and frequent ID switches. This study proposes a Chinese mitten crab tracking method based on YOLOv11-BoT-SORT, including target detection, target tracking, and activity level estimation. In the detection stage, YOLOv11 serves as the baseline network, with the MobileOne convolution module introduced to reduce computation and parameters; the EMA (Excitation and Modulation Attention) mechanism is added to enhance dorsal shell feature extraction, maintaining model accuracy. In the tracking stage, the BoT-SORT algorithm is used, with ResNeST50 network extracting dorsal shell appearance features to improve tracking precision and reduce ID switches. For activity level estimation, the crab’s speed, turning angle, moving distance, and activity time ratio within a specified period are calculated, then combined to determine activity level. Experiments on a self-built dataset show a detection mAP50 of 99.1%, model size of 7.03 MB, a 28.8% reduction compared to the original YOLOv11. The tracking method achieves a MOTA of 88.138%, with IDSW reduced to 3. The average activity level of the Chinese mitten crab was 0.45, with a standard deviation of 0.18, exhibiting an approximately normal distribution. This research enables detection, tracking, and activity level estimation of Chinese mitten crabs, which could have potential applications for aquaculture producers.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148466355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}