TSS-optimized biofloc (200–350 mg/L) enhances survival and antioxidant defense in Cherax quadricarinatus juveniles with reduced aggression and improved water stability

IF 2.4 3区农林科学 Q2 FISHERIES

Aquaculture International Pub Date : 2025-10-17 DOI:10.1007/s10499-025-02291-3

Chengxu Ruan, Junhao Tang, Jiaohong Wang, Ye Tao

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引用次数: 0

Abstract

This study established three total suspended solid (TSS) concentration gradients in biofloc technology (BFT) systems: low (BFTL, ≤ 200 mg/L), medium (BFTM, 200–350 mg/L), and high (BFTH, 350–500 mg/L), with clear water (CW) as control. The 35-day culture performance of redclaw crayfish (Cherax quadricarinatus) juveniles (initial weight 5.58 ± 0.32 mg, density 50 ind./m²) was evaluated. Results demonstrated that the BFTM group achieved the highest survival rate, significantly surpassing the control (p < 0.05), alongside the lowest limb loss rate and significantly narrower weight and length ranges compared to CW (p < 0.05). Antioxidant indicators revealed that BFTM exhibited the highest activities of superoxide dismutase (SOD), catalase (CAT), and total antioxidant capacity (T-AOC), with the lowest malondialdehyde (MDA) content (p < 0.05), indicating effective maintenance of antioxidant function in crayfish. In contrast, the BFTH group showed decreased SOD/CAT activities, elevated MDA levels, and reduced survival, likely due to elevated physiological stress associated with high floc density. Water quality analysis indicated that nitrogenous waste in BFT systems initially accumulated before stabilizing, with all water quality parameters remaining within safe ranges. In conclusion, TSS of 200–350 mg/L optimizes nursery culture by reducing aggression through turbidity-mediated concealment, simultaneously improving cohort uniformity and antioxidant capacity in redclaw crayfish juveniles.

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tss优化后的生物絮团（200 ~ 350 mg/L）可提高中华绒螯蟹幼鱼的存活率和抗氧化防御能力，降低其攻击性，提高其水稳定性

本研究以清水（CW）为对照，在生物絮团技术（BFT）体系中建立了三种总悬浮固体（TSS）浓度梯度：低（BFTL,≤200 mg/L）、中（BFTM, 200 - 350 mg/L）和高（BFTH, 350-500 mg/L）。评价红爪小龙虾（Cherax quadricarinatus）幼鱼（初始体重5.58±0.32 mg，密度50只/m2） 35 d的培养性能。结果显示，BFTM组的存活率最高，明显超过对照组（p < 0.05），肢体丧失率最低，体重和长度范围明显小于CW组（p < 0.05）。抗氧化指标显示，BFTM超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和总抗氧化能力（T-AOC）活性最高，丙二醛（MDA）含量最低（p < 0.05），有效维持了小龙虾的抗氧化功能。相比之下，BFTH组显示SOD/CAT活性降低，MDA水平升高，存活率降低，可能是由于高絮凝密度相关的生理应激升高。水质分析表明，BFT系统中含氮废物在稳定前有初始积累，所有水质参数均在安全范围内。综上所述，200-350 mg/L的TSS通过浊度介导的隐藏减少攻击性，优化了苗圃培养，同时提高了红爪螯虾幼虾的群体均匀性和抗氧化能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.