Australasian Plant Pathology最新文献

Expanding pathogenic diversity in bacterial wilt- insights beyond Ralstonia solanacearum species complex 扩大细菌性青枯病的致病多样性——超越青枯病菌种复群的见解

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2025-08-25 DOI: 10.1007/s13313-025-01066-x

Manisha Saini, Meenu Gupta, Pankaj Kumar, Rahul Saini

引用次数: 0

Inhibitory effects of lemon grass (Cymbopogon citratus) on stem-end rot of mango caused by Lasiodiplodia theobromae 柠檬草对芒果茎端腐病的抑制作用

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2025-08-02 DOI: 10.1007/s13313-025-01065-y

Dionisio de Guzman Alvindia, Mark Anthony Angeles Mangoba

引用次数: 0

First report of Fusarium tanahbumbuense associated with red rot in sugarcane 甘蔗红腐病镰刀菌首次报道

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2025-07-19 DOI: 10.1007/s13313-025-01063-0

Élida Fernanda Cavalcanti Marins, Jaqueline Figueredo de Oliveira Costa, Jackeline Laurentino da Silva, Janaíne Rossane Araújo Silva Cabral, Maria Jussara dos Santos da Silva, Iraíldes Pereira Assunção, Gaus Silvestre de Andrade Lima

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Development of a multiplex RT-PCR assay for simultaneous detection of viruses in cowpea 建立多重RT-PCR同时检测豇豆病毒的方法

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2025-07-19 DOI: 10.1007/s13313-025-01064-z

Layla de Sá Andrade Medeiros, Kelvim Crist Araújo Rocha, Beatriz Meireles Barguil, José Evando Aguiar Beserra Jr

{"title":"Development of a multiplex RT-PCR assay for simultaneous detection of viruses in cowpea","authors":"Layla de Sá Andrade Medeiros, Kelvim Crist Araújo Rocha, Beatriz Meireles Barguil, José Evando Aguiar Beserra Jr","doi":"10.1007/s13313-025-01064-z","DOIUrl":"10.1007/s13313-025-01064-z","url":null,"abstract":"<div>The diagnosis of viral diseases in cowpea is crucial for the efficient implementation of control measures. A multiplex reverse-transcription polymerase chain reaction (RT-PCR) assay was developed for the simultaneous detection of cowpea severe mosaic virus (CPSMV), cucumber mosaic virus (CMV), and cowpea mild mottle virus (CPMMV) in cowpea. The targeted viruses produced the expected amplicons, namely 300 bp for CMV, 500 bp for CPMMV, and 890 bp for CPSMV. The relative analytical sensitivities of the assay was determined through probit analysis of serial dilutions (10⁻¹ to 10⁻⁶), with detection limits at 95% probability established as 10− 2 for all viruses in multiplex RT-PCR. The performance of multiplex RT-PCR was confirmed by uniplex RT-PCR for each target virus. The multiplex RT-PCR of field cowpea leaf samples revealed a high incidence of CMV and CPSMV, and no infection by CPMMV. Approximately 24% of the samples were simultaneously infected with CPSMV and CMV. No other comovirus, besides CPSMV, was detected, when the samples were tested with degenerate primers for comoviruses by uniplex RT-PCR. This assay streamlines the diagnosis of CPSMV, CMV, and CPMMV by offering a cost-effective and efficient tool. Additionally, it supports epidemiological studies of these pathogens, providing critical data to inform effective control strategies.</div>","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":"54 4","pages":"479 - 487"},"PeriodicalIF":1.1,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145122170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of Puccinia xanthii Schw. causing rust on Xanthium strumarium L. in India 黄腐菌首次报道。在印度引起苍耳菌锈病

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2025-07-08 DOI: 10.1007/s13313-025-01061-2

Rageshwari Selvaraj, Rajasulochana Nallan, Sundar Manavalan, Vinod Kumar Selvaraj

引用次数: 0

First report of highly pathogenic Cladosporium colombiae isolates on durum wheat plant in Algeria 阿尔及利亚硬粒小麦株高致病性哥伦比亚枝孢菌分离株首次报道

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2025-07-01 DOI: 10.1007/s13313-025-01062-1

Gharzouli Asma, Bencheikh Amor, Sadrati Nouari, Daichi Barkahoum Meriem, Rouag Noureddine

引用次数: 0

First report of Colletotrichum species causing chili (Capsicum annuum L.) anthracnose in Hue, Vietnam 越南顺化地区引起辣椒炭疽病的炭疽菌属首次报道

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2025-06-23 DOI: 10.1007/s13313-025-01060-3

Nguyen Thi Thuy Tien, Le Thi Ha Thanh, Nguyen Thi Kim Thi, Phung Thi Bich Hoa, Nguyen Quang Duc Tien, Nguyen Hoang Loc

{"title":"First report of Colletotrichum species causing chili (Capsicum annuum L.) anthracnose in Hue, Vietnam","authors":"Nguyen Thi Thuy Tien, Le Thi Ha Thanh, Nguyen Thi Kim Thi, Phung Thi Bich Hoa, Nguyen Quang Duc Tien, Nguyen Hoang Loc","doi":"10.1007/s13313-025-01060-3","DOIUrl":"10.1007/s13313-025-01060-3","url":null,"abstract":"<div>Anthracnose is one of the causes of significant reductions in chili yield and marketability worldwide. In Vietnam, anthracnose is also one of the most dangerous diseases of chili. This work aimed to identify Colletotrichum species causing chili anthracnose in Hue based on phylogenetic analysis of multiple locus genes such as ITS, act, cal, chs-1, gapdh, and tub2. The study determined 3 species of Colletotrichum, which were C. scovillei, C. truncatum, and C. siamense. Furthermore, the morphological features of these species were determined. The pathogenicity assays revealed that these species displayed different levels of virulence on artificially infected fruits such as chili, banana, mango, and dragon fruit. The incidence and lesion size of anthracnose disease in wounded fruits were higher than in unwounded fruits. Notably, the pathogenicity of C. truncatum on banana has not been previously reported and it. warns the banana industry that anthracnose caused by this species may appear on this fruit crop. Our study provides preliminary insights into the Colletotrichum species associated with chili anthracnose, which will assist in developing biocontrol strategies and managing post-harvest diseases that impact chili production in Vietnam.</div>","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":"54 3","pages":"413 - 424"},"PeriodicalIF":1.1,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145167984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating antofine's dual effects: Controlling Sclerospora graminicola infection while improving physiological performance and yield in foxtail millet 评价安托芬对谷子生理性能和产量的双重影响：控制谷粒核孢子菌感染

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2025-06-19 DOI: 10.1007/s13313-025-01059-w

Xin Gui, Jiaojiao Lu, Mei Wang, Yanbo Huo, Jing Wang, Mingqing Liu, Zhijun Zhang

{"title":"Evaluating antofine's dual effects: Controlling Sclerospora graminicola infection while improving physiological performance and yield in foxtail millet","authors":"Xin Gui, Jiaojiao Lu, Mei Wang, Yanbo Huo, Jing Wang, Mingqing Liu, Zhijun Zhang","doi":"10.1007/s13313-025-01059-w","DOIUrl":"10.1007/s13313-025-01059-w","url":null,"abstract":"<div>Sclerospora graminicola, a destructive oomycete pathogen, is the causal agent of downy mildew in foxtail millet (Setaria italica L.), a devastating disease that severely impacts crop productivity. Antofine, an alkaloid isolated from Cynanchum komarovii Al. Iljinski, exhibits significant inhibitory effects on S. graminicola. This study evaluated the effects of 2% antofine suspension (applied via seed coating) on physiological traits, nutritional quality, and yield in foxtail millet. The results revealed that 2% antofine treatment significantly enhanced plant growth parameters, including plant height, total root length, and root-shoot ratio during both seedling and jointing stages. The enzymatic analysis revealed that 2% antofine treatment induced peak activity of key defense-related enzymes at the elongation stage, with catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL) showing significant increases of 11.06%, 34.70%, 345.67%, and 8.68%, respectively, compared to the control. Field trials demonstrated a 66.81% disease control efficacy, accompanied by a 22.01% yield increase (4245.58 kg/ha). Notably, antofine application significantly improved grain quality, elevating total sugar (8.26%), soluble sugar (67.32%), yellow pigment (12.04%), vitamin C (46.29%) and crude protein (30.64%) contents. These results collectively indicate that antofine functions as a multifunctional agent that not only suppresses S. graminicola infection but also activates plant defense mechanisms, enhances stress tolerance, and improves crop productivity and nutritional value. The study provides compelling evidence for antofine's dual role as both an effective biocontrol agent and a plant growth regulator in foxtail millet cultivation systems.</div>","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":"54 3","pages":"399 - 411"},"PeriodicalIF":1.1,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145167426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of Neopestalotiopsis spp. (grey leaf spot disease) on Persea bombycine hook (Som plant) in Assam, India 印度阿萨姆邦Persea bombyine hook （Som植物）上Neopestalotiopsis spp.（灰叶斑病）的分子特征

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2025-06-17 DOI: 10.1007/s13313-025-01045-2

Bhrigupoti Hazarika, Nilutpal Saikia, Ajit K. Tamuli, Palash Debnath

{"title":"Molecular characterization of Neopestalotiopsis spp. (grey leaf spot disease) on Persea bombycine hook (Som plant) in Assam, India","authors":"Bhrigupoti Hazarika, Nilutpal Saikia, Ajit K. Tamuli, Palash Debnath","doi":"10.1007/s13313-025-01045-2","DOIUrl":"10.1007/s13313-025-01045-2","url":null,"abstract":"<div>\u0000 \u0000 Northeast India, particularly Assam, is a significant region for sericulture, home to the renowned Muga silkworms (Antheraea assamensis Helfer) that produce golden-hued silk. The primary feeding plants for these silkworms are Som (Persea bombycine King ex Hook. Fil. Kosterm) and Soalu (Litsea monopetala Juss). However, these plants are susceptible to diseases, including grey leaf spot, which affect leaf quality and silkworm productivity. This study aimed to identify the pathogen causing grey leaf spot on Som plants. Diseased leaf samples were collected from various locations in Assam, and fungal isolates were designated and analyzed using PCR with ITS-1 and ITS − 4 primers. The isolate BP2, identified through sequence analysis and BLAST®, showed the highest homology (92.6%) with Neopestalotiopsis spp. strain NTUCC (accession number MT322112). The results were submitted, and the accession number OQ195903 was assigned by NCBI. Pathogenicity tests also confirm the pathogenic effect of the test isolate BP2. This study provides crucial insights into the pathogen responsible for grey leaf spot, aiding in developing management strategies for sericulture sustainability.\u0000 </div>","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":"54 3","pages":"317 - 320"},"PeriodicalIF":1.1,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145166096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods for quantifying low populations of root-knot nematode (Meloidogyne spp.) in vegetable soils and their potential for use in monitoring programs to improve nematode management decisions 蔬菜土壤中根结线虫（Meloidogyne spp.）低种群的量化方法及其在监测项目中用于改善线虫管理决策的潜力

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2025-06-17 DOI: 10.1007/s13313-025-01058-x

Graham Roy Stirling, Neil L. Wilson, Jennifer A. Cobon, Wayne T. O’Neill

{"title":"Methods for quantifying low populations of root-knot nematode (Meloidogyne spp.) in vegetable soils and their potential for use in monitoring programs to improve nematode management decisions","authors":"Graham Roy Stirling, Neil L. Wilson, Jennifer A. Cobon, Wayne T. O’Neill","doi":"10.1007/s13313-025-01058-x","DOIUrl":"10.1007/s13313-025-01058-x","url":null,"abstract":"<div>Most Australian vegetable growers apply fumigants or nematicides as a precautionary nematode control measure when crops susceptible to root-knot nematode (RKN, Meloidogyne spp.) are grown in soils and environmental conditions suitable for the nematode. The only way growers can make rational decisions on whether these expensive and environmentally disruptive chemicals are required is to regularly monitor RKN populations and decide whether numbers prior to planting are high enough to cause economic damage. However, such monitoring programs are difficult to implement because nematode quantification methods vary in efficiency and the damage threshold for RKN on highly susceptible vegetable crops is often < 10 root-knot nematodes /200 mL soil. Consequently, five nematode quantification methods were tested to see whether they could reliably detect these very low population densities of RKN. Two novel methods produced consistent results: 1) extracting nematodes from 2 L soil samples using Whitehead trays, quantifying the RKN DNA in the nematode suspension using molecular methods, and generating a standard curve so that the molecular results provided an estimate of the total number of RKN individuals in the sample, and 2) a bioassay in which two tomato seedlings were planted in pots containing 2 L soil and the number of galls produced on roots were counted after 21–25 days. Both methods could be used to quantify low populations of RKN, but bioassays are more practical because expensive equipment and facilities are not required and they can be done at a local level by people lacking nematological or molecular skills.</div>","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":"54 3","pages":"387 - 398"},"PeriodicalIF":1.1,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145166753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0