Food Research International最新文献

{"title":"CiLi (Rosa roxburghii Tratt.) polyphenols improve colitis via gut microbiota-lipid mediator-immunity axis","authors":"Huanzhi Yang,&nbsp;Yang Zhao,&nbsp;Ruiqi Zhang,&nbsp;Liang Zhao,&nbsp;Haixia Yang,&nbsp;Xiaojun Liao","doi":"10.1016/j.foodres.2025.116257","DOIUrl":"10.1016/j.foodres.2025.116257","url":null,"abstract":"<div><div>Dysbiosis of gut microbiome is one of the most important factors leading to inflammatory bowel disease (IBD). Intake of phytochemicals from fruits and vegetables is an effective way to improve IBD, but how these bioactivators regulate gut microbiota to exert healthy effects remains unclear. Here, we found that pretreatment with CiLi juice, particularly its polyphenol component, alleviated dextran sulfate sodium (DSS)-induced colitis while preserving intestinal barrier integrity. CiLi polyphenols (CL_PP) reduced inflammation and oxidative stress in colon tissue and enriched fecal short-chain fatty acids. Importantly, CL_PP significantly regulated the gut microbiome diversity, increasing beneficial bacteria (e.g., <em>Clostridia_UCG-014</em>, <em>f_Muribaculaceae</em> and <em>Ileibacterium_valens)</em> while decreasing harmful bacteria (<em>Escherichia-Shigella</em> and <em>Romboutsia)</em>. Multiomics analysis revealed that CL_PP upregulated bioactive lipid metabolites, particularly those derived from polyunsaturated fatty acids (e.g., resolvin D2, prostaglandin A1, and glycerophosphocholine) related gene expressions (<em>Pltp</em>, <em>Alox15</em> and <em>Pld4)</em>. Additionally, CL-PP downregulated the oxidative stress markers (oxidized glutathione and glutathione peroxidase 3)<em>,</em> and immune cell markers (CD8 and CD68)<em>.</em> Fecal microbiota transplantation confirmed that the fecal microbiota from CL_PP-treated mice exhibited anti-colitis effects. These effects were diminished in antibiotic-treated mice, underscoring the importance of the gut microbiota in mediating the CL_PP's anti-inflammatory benefits. This study suggests that CL_PP is a potential modulator of gut microbiome dysbiosis for the prevention and treatment of colitis.</div></div>","PeriodicalId":323,"journal":{"name":"Food Research International","volume":"209 ","pages":"Article 116257"},"PeriodicalIF":7.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“Growth Predictor”: A new predictive modelling and quantitative microbial risk assessment tool","authors":"Panagiotis N. Skandamis","doi":"10.1016/j.foodres.2025.116329","DOIUrl":"10.1016/j.foodres.2025.116329","url":null,"abstract":"<div><div>A new predictive modelling and quantitative microbial risk assessment (QMRA) software, developed in R, is available on-line (<span><span>https://skandamis.shinyapps.io/Microbial-Growth-Predictor-Dashboard/</span><svg><path></path></svg></span>). Primary model fitting is carried out with the Baranyi model. Secondary fitting and growth simulations are based on gamma models with or without interactions. The same gamma terms can be used for fitting and growth simulations under static or dynamic conditions. One of the novel features is the use of normal distributions to describe the variability in T, pH, a_w, the levels of a single inhibitor and the inter-strain variability in growth limits. The QMRA is comprised of four consecutive modules from primary production until consumption. In addition to prevalence, the modules may also consider partition, mixing and cross-contamination. Variability can be introduced through a variety of probability distributions, for initial contamination, re-contamination, storage time and temperature, product characteristics, serving size and maximum population density. Fixed or variable log reductions during cooking, may be introduced as user-defined values or probability distributions, respectively, or estimated by a Bigelow thermal inactivation model. The trilinear primary growth model is used for estimating log changes, based on <em>μ</em>_<em>max</em> obtained by gamma models. The QMRA outputs include graphical distribution of ingested dose and probability of illness (<em>P</em>_<em>ill</em>), as well as tabular estimates. The user may select built-in dose-response models, or define the parameters values of exponential, beta-Poisson, beta-binomial and binomial dose-response models. The tool is intended for multiple users from the scientific community, the authorities and the food industry, for growth simulations and high-resolution risk assessments.</div></div>","PeriodicalId":323,"journal":{"name":"Food Research International","volume":"209 ","pages":"Article 116329"},"PeriodicalIF":7.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current applications of benchtop FT-NMR in food science: From quality control to adulteration detection","authors":"Giacomo Di Matteo ,&nbsp;Silvia Grassi ,&nbsp;Maria Carmela Emanuele ,&nbsp;Giuseppe Scioli ,&nbsp;Federico Ivan Brigante ,&nbsp;Luana Bontempo ,&nbsp;Cinzia Ingallina ,&nbsp;Claude Guillou ,&nbsp;Anatoly P. Sobolev ,&nbsp;Luisa Mannina","doi":"10.1016/j.foodres.2025.116327","DOIUrl":"10.1016/j.foodres.2025.116327","url":null,"abstract":"<div><div>The introduction of benchtop FT-NMR spectrometers in recent years represents a remarkable innovation in various fields, including the food sector. Modern benchtop FT-NMR spectrometers are low-field instruments, with a magnetic field ranging from 1 T to 2.35 T (¹H resonance frequency from 43 MHz to 100 MHz), characterized by compact design, ease of use, and low maintenance costs. As in the case of high-field NMR instruments, benchtop NMR spectra (obtained by Fourier transformation) contain important information useful for compound identification and quantification.</div><div>In this review, a description of the fundamental steps useful both to acquire benchtop NMR spectra and to treat the obtained data is reported together with a wide range of applications in the food field. In particular, peculiar aspects of commercial benchtop instruments as well as NMR data acquisition, processing and treatment are reviewed reporting also a practical pipeline and a list of good practices for benchtop NMR applications. Benchtop FT-NMR applications, mainly focused on food adulteration detection and quality control, are discussed here using targeted, metabolomic, and fingerprinting approaches. Finally, the industrial applicability of benchtop NMR methods in either static or continuous mode is reported.</div></div>","PeriodicalId":323,"journal":{"name":"Food Research International","volume":"209 ","pages":"Article 116327"},"PeriodicalIF":7.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive review on human Milk oligosaccharides: Biosynthesis, structure, intestinal health benefits, immune regulation, neuromodulation mechanisms, and applications","authors":"Jieting Yu ,&nbsp;Le Li ,&nbsp;Supaluck Kraithong ,&nbsp;Lingshan Zou ,&nbsp;Xiaoyong Zhang ,&nbsp;Riming Huang","doi":"10.1016/j.foodres.2025.116328","DOIUrl":"10.1016/j.foodres.2025.116328","url":null,"abstract":"<div><div>This review provides a comprehensive analysis of the biosynthetic pathways of various oligosaccharides in <em>Escherichia coli</em>, structural characteristics, and bioactive mechanisms of human milk oligosaccharides (HMOs), with a particular emphasis on their roles in gut health, immune modulation, and neurodevelopment. HMOs primarily function as prebiotics, facilitating the growth of beneficial bacteria such as <em>Bifidobacterium</em> to maintain microbial homeostasis, with a discussion on the synergistic role of carbohydrate-binding modules (CBMs). In immune modulation, HMOs interact with lectins on immune and epithelial cells, influencing immune responses via pathways such as Toll-like receptors (TLRs). Additionally, HMOs have been linked to enhanced cognitive, motor, and language development in infants, influencing genes such as <em>GABRB2, SLC1A7, GLRA4,</em> and <em>CHRM3</em>. The review also examines commercially available HMO-containing products and highlights future research directions and potential applications in nutrition and healthcare.</div></div>","PeriodicalId":323,"journal":{"name":"Food Research International","volume":"209 ","pages":"Article 116328"},"PeriodicalIF":7.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of chitosan‑calcium coating on the physiological characteristics and browning of Stropharia rugosoannulata during postharvest storage","authors":"Yingqi Zhang ,&nbsp;Xi Feng ,&nbsp;Defang Shi ,&nbsp;Xiaoju Tian ,&nbsp;Wen Huang ,&nbsp;Ying Liu","doi":"10.1016/j.foodres.2025.116226","DOIUrl":"10.1016/j.foodres.2025.116226","url":null,"abstract":"<div><div>The effects of chitosan‑calcium (CTS<img>Ca) coating on the physiological characteristics and browning of fresh <em>Stropharia rugosoannulata</em> were investigated. The 0.5 % CTS-Ca coating reduced the weight loss rate, opening rate, and browning of mushrooms. CTS-Ca could decrease the contents of total phenols and the activities of enzymes associated with enzymatic browning, increase the activities of antioxidant enzymes, and reduce the production of malondialdehyde, a product of membrane lipid metabolism. Based on transcriptomic analysis, CTS-Ca treatment down-regulated the expression of genes on the chorismate/tyrosine pathway to inhibit the synthesis of phenolic substrates and tyrosinase activity, up-regulated the three energy metabolism pathways of glycolysis, TCA cycle, and oxidative phosphorylation to maintain the integrity of the cell membrane, increased the expression of catalase and superoxide dismutase genes to scavenge ROS. Our results would provide a simple and practical preservation solution for <em>S. rugosoannulata</em> during the postharvest storage.</div></div>","PeriodicalId":323,"journal":{"name":"Food Research International","volume":"209 ","pages":"Article 116226"},"PeriodicalIF":7.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermentation of Amazonian fruit pulp (bacaba) with distinct probiotics: Impacts on chemical composition, bioaccessibility, and effects on human intestinal microbiota","authors":"Bianca Beatriz Torres de Assis ,&nbsp;Lucélia Cabral ,&nbsp;Francyeli Araújo Silva ,&nbsp;Jaqueline de Araújo Bezerra ,&nbsp;Melline Fontes Noronha ,&nbsp;Hubert Vidal ,&nbsp;Marcos dos Santos Lima ,&nbsp;Tatiana Colombo Pimentel ,&nbsp;Marciane Magnani","doi":"10.1016/j.foodres.2025.116326","DOIUrl":"10.1016/j.foodres.2025.116326","url":null,"abstract":"<div><div>Bacaba (<em>Oenocarpus bacaba</em> Mart.) is an underexplored Amazonian fruit rich in polyphenols that can serve as a substrate for probiotic survival and may positively impact on the composition and metabolism of the intestinal microbiota. This study aimed to evaluate the bacaba pulp fermented with probiotics <em>Lactobacillus acidophilus</em> 05 (LA-05) and <em>Lacticaseibacillus casei</em> 01 (LC1) regarding the chemical composition and probiotics survivability during fermentation (48 h), and the effect on the modulation of the intestinal microbiota of healthy adults through 16S rRNA sequencing. The probiotic-fermented bacaba pulps showed decreased pH and total soluble solids values and sugar content (maltose, glucose, fructose, and rhamnose), and increased titratable acidity values, organic acid content (lactic and tartaric acids), and phenolic compounds concentration compared to the control pulp. Furthermore, it presented adequate probiotic viability after fermentation and simulated gastrointestinal conditions. The bacaba pulp fermented with LC1 showed a higher concentration of butyric acid and phenolic compounds concentration (trans-resveratrol, cis resveratrol, catechin, procyanidin B₂, and pelargonidin 3-glucoside) and bioaccessibility compared to the control pulp. The bacaba pulp fermented with LA-5 showed a higher concentration of pelargonidin 3-glucoside and procyanidin B₂ compared to the control pulp and the highest bioaccessibility of some phenolic compounds (trans-resveratrol, cis-resveratrol, catechin, epicatechin, procyanidin B₁, procyanidin B₂, myricetin, and isorhamnetin). <em>In vitro</em> fecal fermentation reduced the pH and increased the abundance of <em>Desulfovibrionales</em>, <em>Lactobacillales,</em> and <em>Peptostreptococcales-Tissierellales</em> for all treatments. Bacaba pulp with LC1 resulted in the lowest pH values, and increased production of organic acids and concentration of phenolic compounds. Furthermore, both probiotic pulps increased the abundance of <em>Lactobacillales</em> and <em>Acidaminococcales</em> and decreased the abundance of <em>Clostridiales.</em> These findings provide new information about the potential of using bacaba in a functional pulp that may benefit human health through colonic microbiota changes.</div></div>","PeriodicalId":323,"journal":{"name":"Food Research International","volume":"209 ","pages":"Article 116326"},"PeriodicalIF":7.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous melatonin enhancing the accumulation of flavonoids and carotenoids in wolfberry fruit at cold storage","authors":"Rong Jiang ,&nbsp;Zhiqiang Wang ,&nbsp;Yixin Jia ,&nbsp;Biao A. ,&nbsp;Yuan Zong ,&nbsp;Chao Yang ,&nbsp;Minglin Li ,&nbsp;Hongjiao Wang ,&nbsp;Yuling Zhao ,&nbsp;Ying Wang ,&nbsp;Shaohua Zeng","doi":"10.1016/j.foodres.2025.116320","DOIUrl":"10.1016/j.foodres.2025.116320","url":null,"abstract":"<div><div>The deterioration of fruit quality during refrigeration is a typical symptom of the storage and transportation of <em>Lycium barbarum</em> L. (wolfberry) fruit after harvest. This study aimed to explore the impact of melatonin (MT) treatment on the fruit quality of wolfberry stored at 4°C, using combined transcriptomics and metabolomics analyses. In this study, about 15% of the flavonoid metabolites differed significantly after MT treatment in yellow-fleshed wolfberry, especially for flavanols, flavanones, and flavonols. MT treatment enhanced esterified carotenoid content in red-fleshed wolfberry postharvest. Comparative transcriptomic analysis revealed that MT-induced upregulation of <em>LbaF3’H</em>, <em>LbaHQT</em> and <em>LbaCHS1</em> might contribute to the increased flavonoids in yellow-flesh wolfberry, and that MT-induced upregulation of <em>LbaPSY</em>, <em>LbaCRTISO</em> and <em>LbaCYCB</em> are responsible for the increased carotenoids in red-flesh wolfberry. Coexpression network analysis showed that several transcription factors such as <em>LbaGATA</em> are involved in MT-mediated regulation of flavonoids and carotenoids. Dual luciferase assay evidenced that LbaGATA and LbaRKD5 depress the expression of <em>LbaPSY</em>. Overall, this study underscores MT's role in maintaining wolfberry postharvest quality during storage, providing a solid foundation of food application.</div></div>","PeriodicalId":323,"journal":{"name":"Food Research International","volume":"209 ","pages":"Article 116320"},"PeriodicalIF":7.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication and characterization of multifunctional bioactive aerogel pads as superabsorbent - Case study: Meat preservation","authors":"Forouzan Kurd,&nbsp;Milad Fathi,&nbsp;Mohammad Shahedi,&nbsp;Nafiseh Soltanizadeh","doi":"10.1016/j.foodres.2025.116268","DOIUrl":"10.1016/j.foodres.2025.116268","url":null,"abstract":"<div><div>In this study cellulose nanofibrils (CNFs)/poly vinyl alcohol (PVA) aerogels were produced and the effect of the concentration of CNF/PVA on porosity, density, pore size, water absorption, water vapor absorption, and mechanical properties were studied. CNFs were developed using peanut shells as agricultural waste. Curcumin (CUR) was incorporated into the aerogel matrix at different concentrations (30 %, 40 %, and 50 %). The best bioactive aerogel pad was selected based on its encapsulation efficiency (80.57 ± 0.29 %), loading capacity (22.85 ± 0.22 %), and antioxidant properties (74.14 ± 0.31 %). The aerogels loaded with CUR were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis. The bioactive aerogel pads showed antibacterial properties against <em>Staphylococcus aureus</em>. The cumulative release (65 %) of CUR from the aerogel matrix for 8 days, indicates a sustained release in meat-simulated media, which is suitable for increasing the shelf life of meat. The kinetics of the release showed that the Ritger-Peppas and Peppas-Sahlin models are the best models for the meat-simulated media. The storage results indicated that the package containing the aerogels loaded with CUR could increase the shelf life of meat from 6 to 8 days compared to the package containing the aerogels without CUR and the control package. Finally, this research provides a suitable strategy in the field of food packaging to produce multifunctional antimicrobial pads based on biodegradable agricultural waste.</div></div>","PeriodicalId":323,"journal":{"name":"Food Research International","volume":"209 ","pages":"Article 116268"},"PeriodicalIF":7.0,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular interactions and binding mechanisms of the homogalacturonan and rhamnogalacturonan-I fractions of pectin and different anthocyanins","authors":"Ying Xing ,&nbsp;Jiahui Yan ,&nbsp;Shichun Ji ,&nbsp;Xingyi Li ,&nbsp;Liping Kou ,&nbsp;Jun Wang ,&nbsp;Huaide Xu","doi":"10.1016/j.foodres.2025.116298","DOIUrl":"10.1016/j.foodres.2025.116298","url":null,"abstract":"<div><div>This study investigated the interactions between different structural regional fractions of pectin, specifically homogalacturonan (HG) and rhamnogalacturonan-I (RG-I) fractions, and three anthocyanin monomers: cyanidin-3-O-glucoside (C3G), peonidin-3-O-glucoside (P3G), and pelargonidin-3-O-malonylglucoside (P3M). The binding ratios, physicochemical and structural characteristics of these pectin-anthocyanin complexes, and their binding mechanisms were analyzed. The HG fraction exhibited the highest binding ratio with C3G. Zeta potential and particle size analyses indicated that the HG fraction had a significantly higher negative charge compared to RG-І at pH 3.7, and the complexes exhibited an increase in particle size relative to the individual pectin fractions. Furthermore, data from ¹H NMR and isothermal titration calorimetry (ITC) confirmed that hydrogen bonding, electrostatic interactions, and hydrophobic interactions primarily facilitated the binding interactions. In the binding interactions involving HG/RG-І and C3G/P3G, the Δδ_max, binding constant, and Gibbs free energy (ΔG) of the HG fraction were greater than those of the RG-І fraction, while C3G exhibited stronger binding characteristics than P3G. However, the RG-І fraction demonstrated stronger binding to P3M than to HG. The interactions between pectin and anthocyanins are highly dependent on the structural characteristics of both components. These results offer further insights into the interactions between pectin fractions and anthocyanins at the molecular level.</div></div>","PeriodicalId":323,"journal":{"name":"Food Research International","volume":"209 ","pages":"Article 116298"},"PeriodicalIF":7.0,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synbiotics of encapsulated Limosilactobacillus fermentum K73 promotes in vitro favorable gut microbiota shifts and enhances short-chain fatty acid production in fecal samples of children with autism spectrum disorder","authors":"Katherine Bauer Estrada ,&nbsp;Natalia Conde-Martínez ,&nbsp;Alejandro Acosta-González ,&nbsp;Luis Eduardo Díaz-Barrera ,&nbsp;Gina Paola Rodríguez-Castaño ,&nbsp;María Ximena Quintanilla-Carvajal","doi":"10.1016/j.foodres.2025.116227","DOIUrl":"10.1016/j.foodres.2025.116227","url":null,"abstract":"<div><div>Modulation of the gut microbiota has emerged as a promising approach for addressing the gastrointestinal and neurodevelopmental symptoms associated with autism spectrum disorder (ASD). Consequently, this study aimed to evaluate the impact of four formulated synbiotics comprising <em>Limoscilactobacillus fermentum</em> K73, high-oleic palm oil and whey, on the gut microbiota composition of Colombian children with and without ASD. These components were encapsulated through high-shear emulsification and spray drying. The four synbiotics and their individual components were subjected to <em>in vitro</em> digestion and fermentation using samples of Colombian children gut microbiota. Short-chain fatty acids (SCFAs), including lactic, acetic, propionic, and butyric acids, were quantified using HPLC-DAD, while serotonin was determined by an ELISA kit after <em>in vitro</em> fermentations. Changes in microbial structure were assessed by the sequencing of the 16S rRNA gene <em>via</em> next-generation sequencing (NGS). The results revealed a decrease in the abundance of genera like <em>Bacteroides</em> and <em>Dorea</em> in ASD-associated samples after the treatment with the synbiotics. Conversely, an increase in the relative abundance of probiotic-related genera, including <em>Lactobacillus, Streptococcus</em>, and <em>Anaerostipes</em>, was observed. Furthermore, the analysis of SCFAs and serotonin indicated that the synbiotic intervention resulted in an elevated butyric acid and microbial serotonin synthesis, alongside a decrease in propionic acid, which is changes considered beneficial in the context of ASD. This evidence suggests that synbiotics of <em>L. fermentum</em> K73 could represent a promising live biotherapeutic strategy for modulating the gut microbiota of children with ASD.</div></div>","PeriodicalId":323,"journal":{"name":"Food Research International","volume":"209 ","pages":"Article 116227"},"PeriodicalIF":7.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}