Molecular Nutrition & Food Research最新文献

Sea Cucumber Peptides Promote Testosterone Synthesis in Male Mice: Possibly via Alistipes-Bile Acid-FXR Signaling Pathway. 海参肽促进雄性小鼠睾酮合成：可能通过alistips -胆汁酸- fxr信号通路。

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-10-07 DOI: 10.1002/mnfr.70286

Rongbo Pan,Ziying Min,Kailin Li,Fengqin Feng,Baodong Zheng,Xianliang Luo

{"title":"Sea Cucumber Peptides Promote Testosterone Synthesis in Male Mice: Possibly via Alistipes-Bile Acid-FXR Signaling Pathway.","authors":"Rongbo Pan,Ziying Min,Kailin Li,Fengqin Feng,Baodong Zheng,Xianliang Luo","doi":"10.1002/mnfr.70286","DOIUrl":"https://doi.org/10.1002/mnfr.70286","url":null,"abstract":"In this study, the effects of sea cucumber peptides (SCP) on hormonal regulation in male mice and their underlying mechanisms were explored. SCP was administered to mice, followed by in vivo mating experiments, serum hormone profiling, gene expression analysis, and intestinal microbiome characterization. The impact of SCP on key intestinal bacteria was further validated using in vitro antibacterial assays. In vivo, SCP effectively improved mating ability, increased serum levels of testosterone, and enhanced the expression of testosterone synthesis-related genes. Furthermore, these phenomena may be related to the significant inhibition of Alistipes in the colon of mice by SCP. In vitro, SCP demonstrated antimicrobial activities against Alistipes, characterized by disruption of its cellular membrane integrity and suppression of bile salt hydrolase (BSH) activity. Consistently, there was a significant increase in serum total conjugated bile acids, accompanied by reduced fecal BSH activity and downregulation of farnesoid X receptor (FXR) expression in the testis. Correlation analysis suggested that SCP may promote testosterone synthesis through the Alistipes-bile acid-FXR signaling pathway. Conclusively, SCP can promote testosterone synthesis in male mice through the gut-testosterone axis, providing a new perspective for the study of active peptide-regulated hormones.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"39 1","pages":"e70286"},"PeriodicalIF":5.2,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sulfated Polysaccharides From Gracilaria gracilis (Red Seaweed) Restores Testicular Glucose Metabolism by Improving FSH and Insulin Signaling in Rats With Type 2 Diabetes 红藻硫酸酸化多糖通过改善2型糖尿病大鼠FSH和胰岛素信号恢复睾丸糖代谢

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-10-06 DOI: 10.1002/mnfr.70280

Ochuko L. Erukainure, Tosin A. Olasehinde, Olanrewaju S. Olayeriju, Bukola Omotoso, Md. Shahidul Islam, Ademola O. Olaniran

{"title":"Sulfated Polysaccharides From Gracilaria gracilis (Red Seaweed) Restores Testicular Glucose Metabolism by Improving FSH and Insulin Signaling in Rats With Type 2 Diabetes","authors":"Ochuko L. Erukainure, Tosin A. Olasehinde, Olanrewaju S. Olayeriju, Bukola Omotoso, Md. Shahidul Islam, Ademola O. Olaniran","doi":"10.1002/mnfr.70280","DOIUrl":"https://doi.org/10.1002/mnfr.70280","url":null,"abstract":"Testicular glucose homeostasis plays important roles in testicular energy metabolism and male fertility, but it is altered in type 2 diabetes (T2D), leading to male infertility. In the present study, the therapeutic effect of sulfated polysaccharides (SPCs) from Gracilaria gracilis on testicular glucose metabolism was investigated in T2D rats. SPCs were administered to two groups of T2D rats at 150 and 300 mg/kg bodyweight, respectively. The T2D control group was administered water only, while metformin served as the control drug administered to the standard treatment group. Non‐T2D rats served as the normal group. After 5 weeks of treatment, the rats were sacrificed and their testes harvested and analyzed for insulin, FSH, glucose, and glutathione metabolisms. Treatment with SPCs led to a significant increase in insulin, IRS‐1, FSH, GLUT4, GSH, ATP levels, hexokinase, glucose 6‐phisphate dehydrogenase, glyoxalase, glutathione reductase, and glutathione peroxidase activities, while improving testicular morphology. SPCs further led to significant depletion in glycogen phosphorylase, glucose 6‐phosphatase, fructose‐1,6‐biphosphatase, aldose reductase, polyol dehydrogenase, ATPase, and ENTPDase activities. These results indicate the potentials of SPCs from G. gracilis to improve male fertility in T2D. This is evident by its ability to improve insulin‐FSH signaling, glucose, energy metabolisms, and testicular morphology.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"108 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative Network Pharmacology and Proteomics Decipher the Immunomodulatory Mechanism of Sulforaphane Against Intrinsic Skin Aging. 综合网络药理学和蛋白质组学揭示萝卜硫素抗皮肤内在衰老的免疫调节机制。

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-10-02 DOI: 10.1002/mnfr.70281

Xin Du,Xingyu Yang,Chenchen Zhang,Chuyu Fu,Hongkang Zhu

{"title":"Integrative Network Pharmacology and Proteomics Decipher the Immunomodulatory Mechanism of Sulforaphane Against Intrinsic Skin Aging.","authors":"Xin Du,Xingyu Yang,Chenchen Zhang,Chuyu Fu,Hongkang Zhu","doi":"10.1002/mnfr.70281","DOIUrl":"https://doi.org/10.1002/mnfr.70281","url":null,"abstract":"Skin aging is characterized by declines in structural functions, contributing to age-associated frailty. Sulforaphane (SFN), a natural anti-inflammatory substance, has been widely applied in multiple types of cancer therapies. However, its role in alleviating intrinsic skin aging remains to be elucidated. Integrative network pharmacology and proteomics were utilized to investigate the underlying mechanisms of SFN in intrinsic skin aging. Fifty-one anti-aging targets of SFN were identified, highlighting its promising regulatory impact on the aging process. Based on an 18-month-old natural aging mouse model, significant alleviation in skin structure, redox homeostasis, and immune cell composition was noted after 2 months of SFN supplementation. Additionally, proteomic analysis demonstrated that SFN reversed the proteomic profile of intrinsic skin aging, with 233 differentially expressed proteins (DEPs) identified in SFN-fed aging mice. Of note, the up-regulated DEPs were highly enriched in the apelin signaling pathway (p = 0.010). Furthermore, immune cell infiltration and whole blood cell analysis revealed that SFN rescued T cells depletion in dermal tissue, which was strongly correlated with DEPs enriched in the SFN-activated apelin signaling pathway. SFN improves skin morphology and immune functions via activating the apelin signaling pathway, suggesting new prime targets in counteracting intrinsic skin aging.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"120 1","pages":"e70281"},"PeriodicalIF":5.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Mol. Nutr. Food Res. 19'25 发布信息：Mol. Nutr。Food Res. 1925年

IF 4.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-10-02 DOI: 10.1002/mnfr.70267

引用次数: 0

Front Cover: α-Linolenic Acid-Rich Flaxseed Oil Improves Polycystic Ovary Syndrome via Regulating Lipid Metabolism by GPR120-cAMP Pathway and Restoring Gut Microecology 封面：富含α-亚麻酸的亚麻籽油通过GPR120-cAMP途径调节脂质代谢，恢复肠道微生态，改善多囊卵巢综合征

IF 4.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-10-02 DOI: 10.1002/mnfr.70266

Shan Li, Ting Wang, Mei Liu, Ting Ma, Yiwei Li, Jian Liu, Yuanyuan Liu, Wenke Shen, Junbai Ma, Ximin Wang, Xuefeng Han, Hao Wang, Xiaoxia Zhang

引用次数: 0

Metabolomic Profiling and Diet Quality Scoring in a Randomized Crossover Trial of Healthy and Typical Dietary Patterns. 健康和典型饮食模式的随机交叉试验中的代谢组学分析和饮食质量评分。

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-10-01 DOI: 10.1002/mnfr.70271

Jordan Stanford,María Gómez-Martín,Erin Drury Clarke,Jessica Jayne Anne Ferguson,Tracy Lee Burrows,Lisa Gai Wood,Clare Elizabeth Collins

{"title":"Metabolomic Profiling and Diet Quality Scoring in a Randomized Crossover Trial of Healthy and Typical Dietary Patterns.","authors":"Jordan Stanford,María Gómez-Martín,Erin Drury Clarke,Jessica Jayne Anne Ferguson,Tracy Lee Burrows,Lisa Gai Wood,Clare Elizabeth Collins","doi":"10.1002/mnfr.70271","DOIUrl":"https://doi.org/10.1002/mnfr.70271","url":null,"abstract":"This randomized cross-over feeding trial aimed to identify objective metabolomic biomarkers of dietary patterns and assess their relationship with cardiometabolic health. Metabolomic responses were compared between two distinct dietary patterns: the Healthy Australian Diet (HAD) based on national guidelines, and the Typical Australian Diet (TAD) reflecting apparent population intake. Thirty-four healthy adults were provided with all food for each diet pattern for 2 weeks, separated by a washout period. Plasma and spot urine samples were collected pre-post-intervention, and metabolomic profiling was performed using UHPLC-MS/MS. Elastic net regression identified 65 discriminatory metabolites (31 plasma, 34 urine) that distinguished HAD from TAD. A composite diet quality biomarker score derived from these metabolites, was significantly associated with improved cardiometabolic markers, including reductions in systolic and diastolic blood pressure, LDL-cholesterol, triglycerides, and fasting glucose. Several metabolites identified aligned with known food-specific biomarkers, while others represent novel candidates. Distinct short-term metabolomic signatures of a healthy dietary pattern were observed across biofluids. The identified candidate metabolites and biomarker score have potential for translation into objective tools for assessing diet quality in line with the Australian Dietary Guidelines and for early cardiometabolic risk monitoring, pending external validation in independent cohorts. Trial Registration: https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id = 384710.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"71 1","pages":"e70271"},"PeriodicalIF":5.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Anti-Pulmonary Fibrosis Code of Plant Anthocyanins-A Multi-Targeted Anti-Fibrotic Bioactive Compound by Inhibiting the NLRP3 Inflammasome. 植物花青素-一种通过抑制NLRP3炎性体的多靶点抗纤维化生物活性化合物的抗肺纤维化密码。

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-10-01 DOI: 10.1002/mnfr.70275

Xian Tang,Yujing He,Xiaochao Bao,Ziheng Peng,Hongyan Xu,Yin He,Zhongping Wei,Jie Wei

{"title":"The Anti-Pulmonary Fibrosis Code of Plant Anthocyanins-A Multi-Targeted Anti-Fibrotic Bioactive Compound by Inhibiting the NLRP3 Inflammasome.","authors":"Xian Tang,Yujing He,Xiaochao Bao,Ziheng Peng,Hongyan Xu,Yin He,Zhongping Wei,Jie Wei","doi":"10.1002/mnfr.70275","DOIUrl":"https://doi.org/10.1002/mnfr.70275","url":null,"abstract":"Pulmonary fibrosis (PF) is a fatal disease lacking effective treatments. Although anthocyanins possess pleiotropic biological activities, their potential in PF treatment remains insufficiently evaluated. This study intends to systematically assess the anti-fibrotic efficacy of selected anthocyanins and clarify their mechanisms of action.Data mining of six common anthocyanins identified three with the highest anti-fibrotic potential and their therapeutic targets. Animal studies revealed that these anthocyanins ameliorated bleomycin (BLM)-induced PF. Analysis identified 83 common core targets shared between the anthocyanin components and PF pathogenesis, primarily enriched in stimulus response and positive regulation of biological processes. Treatment with all three anthocyanins significantly reduced PF-associated mortality, inhibited weight loss, decreased lung coefficient, and lowered hydroxyproline (HYP) and malondialdehyde (MDA) levels in lung tissue. Furthermore, they suppressed mRNA expression of inflammatory cytokines IL-1β and TNF-α, promoted expression of the epithelial marker E-cadherin, reduced levels of fibrotic markers α-SMA and Collagen I, and modulated the inflammasome NLRP3-Caspase-1 axis ameliorates PF.Anthocyanins, especially anthocyanin-3-O-galactoside (C3G), improve BLM-induced PF by regulating the NLRP3-Caspase-1 inflammasome axis.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"32 1","pages":"e70275"},"PeriodicalIF":5.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Front Cover: Development of a Korean Nutrition Model for In Silico Gut Microbiome Analyses Integrated With Nutrigenomics 封面：韩国营养模型的发展，用于集成营养基因组学的硅肠道微生物组分析

IF 4.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-09-29 DOI: 10.1002/mnfr.70263

Kyoung Su Kim, So-Yeon Yang, Hahyun Jeong, Minji Hong, Jihye Noh, Hong Koh, Dong-Woo Lee

引用次数: 0

Issue Information: Mol. Nutr. Food Res. 18'25 发布信息：Mol. Nutr。Food Res. 18'25

IF 4.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-09-29 DOI: 10.1002/mnfr.70264

引用次数: 0

TMAO Accumulation Induced by Hyperglycemia Triggers Endoplasmic Reticulum Stress in the Mouse Liver. 高血糖诱导的氧化三甲胺积累触发小鼠肝脏内质网应激。

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-09-28 DOI: 10.1002/mnfr.70278

Qiang Gao,Tingting Li,Waleed Al-Ansi,Mingcong Fan,Haifeng Qian,Yan Li,Li Wang

{"title":"TMAO Accumulation Induced by Hyperglycemia Triggers Endoplasmic Reticulum Stress in the Mouse Liver.","authors":"Qiang Gao,Tingting Li,Waleed Al-Ansi,Mingcong Fan,Haifeng Qian,Yan Li,Li Wang","doi":"10.1002/mnfr.70278","DOIUrl":"https://doi.org/10.1002/mnfr.70278","url":null,"abstract":"Excessive carbohydrate intake is a well-established driver of metabolic dysfunction, but its specific role in perturbing hepatic endoplasmic reticulum (ER) homeostasis is not well defined. This study aimed to investigate how dietary carbohydrate levels and the microbial metabolite trimethylamine N-oxide (TMAO) influence hepatic ER stress. In vivo, normal C57BL/6J mice were fed a high-carbohydrate diet (HCD), whereas streptozotocin-induced diabetic mice received a carbohydrate-free ketogenic diet (NCD). In parallel, HepG2 and Huh7 cells were exposed to elevated glucose concentrations and/or TMAO in vitro. HCD feeding impaired glucose tolerance, promoted hepatic lipid accumulation, increased hepatic TMAO content, and elevated markers of FMO3 expression and ER stress (GRP78, p-IRE1). These alterations were reversed by NCD intervention in diabetic mice. In vitro, high glucose and TMAO each induced ER stress in a dose-dependent manner, with combined treatment further activating the IRE1-XBP1 branch and NLRP3 inflammasome signaling. TMAO also increased intracellular reactive oxygen species (ROS) and suppressed Nrf2-mediated antioxidant responses. These findings identify TMAO as a critical mediator linking hyperglycemia to hepatic ER stress and inflammation. Modulating dietary carbohydrate intake and targeting microbial TMAO synthesis may offer promising strategies for alleviating metabolic liver injury.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"53 1","pages":"e70278"},"PeriodicalIF":5.2,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0