Molecular Nutrition & Food Research最新文献

Issue Information: Mol. Nutr. Food Res. 12'25 发布信息：Mol. Nutr。Food Res. 12'25

IF 4.5 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-07-08 DOI: 10.1002/mnfr.70160

引用次数: 0

Lactobacillus rhamnosus PB01 Oral Supplementation Alleviates Metabolic and Reproductive Dysfunctions in Type 1 Diabetic Male Rats. 鼠李糖乳杆菌PB01可减轻1型糖尿病雄性大鼠的代谢和生殖功能障碍。

IF 4.5 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-07-04 DOI: 10.1002/mnfr.70157

Sihan Liu, Hiva Alipour, Malwina Ulanowska, Vladimir Zachar, Fereshteh Dardmeh

{"title":"Lactobacillus rhamnosus PB01 Oral Supplementation Alleviates Metabolic and Reproductive Dysfunctions in Type 1 Diabetic Male Rats.","authors":"Sihan Liu, Hiva Alipour, Malwina Ulanowska, Vladimir Zachar, Fereshteh Dardmeh","doi":"10.1002/mnfr.70157","DOIUrl":"https://doi.org/10.1002/mnfr.70157","url":null,"abstract":"This study investigated the potential therapeutic effects of Lactobacillus rhamnosus PB01 supplementation on metabolic and reproductive dysfunctions in Type 1 diabetes mellitus (T1DM) rat model. Diabetic rats treated with L. rhamnosus PB01 (1 × 10⁹ CFU/day) showed significant improvements in metabolic and reproductive outcomes. Specifically, diabetes-induced weight loss was mitigated by approximately 13%, and the percentage of hyperactivated sperm in the treatment group was significantly 2.5 times higher than that of the diabetic controls. Additionally, testosterone levels were significantly elevated by 27%, further supporting the positive effects on reproductive health. The probiotic supplementation also led to improvements in insulin sensitivity, as evidenced by a 34.12% reduction in homeostasis model assessment index of insulin resistance (HOMA-IR). L. rhamnosus PB01 (DSM 14870) demonstrated potential as an adjunct therapy for managing T1DM-associated complications in the rat model, by improving insulin sensitivity, supporting weight management, and partially restoring diabetes-related fertility impairments, likely through modulation of hormonal pathways. However, further studies are needed to translate the results to humans and refine optimal dosing and supplementation duration for sustained benefits.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":" ","pages":"e70157"},"PeriodicalIF":4.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered Abundance of Butyrate-Producing Lachnospiraceae by Maternal Diet During Pregnancy Potentially Influences MASLD-Related Lipid Dysregulation in Male Rat Offspring. 怀孕期间母体饮食改变产丁酸酯毛缕菌科的丰度可能影响雄性大鼠后代与masld相关的脂质失调。

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-07-02 DOI: 10.1002/mnfr.70153

Soo-Min Kim,Sunwha Park,AbuZar Ansari,Gain Lee,Young Min Hur,Jeongshin An,Sang Suk Lee,Young-Ah You,Young Ju Kim

{"title":"Altered Abundance of Butyrate-Producing Lachnospiraceae by Maternal Diet During Pregnancy Potentially Influences MASLD-Related Lipid Dysregulation in Male Rat Offspring.","authors":"Soo-Min Kim,Sunwha Park,AbuZar Ansari,Gain Lee,Young Min Hur,Jeongshin An,Sang Suk Lee,Young-Ah You,Young Ju Kim","doi":"10.1002/mnfr.70153","DOIUrl":"https://doi.org/10.1002/mnfr.70153","url":null,"abstract":"The maternal diet during pregnancy is an important factor that influences the intrauterine environment during fetal development. However, the relationship among maternal diet, the gut microbiome of offspring, and health outcomes remains unclear. Here, we report that changes in the gut microbiome of offspring after maternal exposure to 50% food restriction and 45% high-fat diet during pregnancy can affect the risk of metabolic dysfunction-associated steatotic liver disease (MASLD) in offspring in a sex-specific manner. Notably, despite no significant difference in body weight, plasma triglyceride and leptin levels were significantly increased in male offspring compared with the controls. The relative abundance of the butyrate-producing genera of the Lachnospiraceae family was dependent on the sex of the offspring and correlated with plasma triglyceride and leptin levels. Interestingly, male offspring in the 50% restricted diet or 45% high-fat diet groups had reduced butyrate levels compared with the control group and were affected by oxidative damage and hepatic lipogenesis. Our findings suggest that the maternal diet during pregnancy affects the gut microbiota of male offspring in a sex-specific manner, potentially predisposing them to MASLD later in life through dysregulation of lipid metabolism.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"3 1","pages":"e70153"},"PeriodicalIF":5.2,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144547932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Moderately High‐Protein Diet During the Critical Development Period Protects Hepatic Functional Integrity and Improves Metabolic Health in Middle‐Aged Offspring via the Gut–Liver Axis 发育关键期适度高蛋白饮食可通过肠-肝轴保护中年后代肝功能完整性并改善代谢健康

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-07-02 DOI: 10.1002/mnfr.70169

Yuqiao Li, Wenyu Xu, Xiangju Kong, Yuanjie Dong, Mengqing An, Yue Guan, Ruohua Wang, He Huang, Songliu Hu, Liying Cai, Yucun Niu

{"title":"A Moderately High‐Protein Diet During the Critical Development Period Protects Hepatic Functional Integrity and Improves Metabolic Health in Middle‐Aged Offspring via the Gut–Liver Axis","authors":"Yuqiao Li, Wenyu Xu, Xiangju Kong, Yuanjie Dong, Mengqing An, Yue Guan, Ruohua Wang, He Huang, Songliu Hu, Liying Cai, Yucun Niu","doi":"10.1002/mnfr.70169","DOIUrl":"https://doi.org/10.1002/mnfr.70169","url":null,"abstract":"A high‐protein diet (HP) in early life has long‐term effects on the metabolism and liver health of offspring. However, few studies focus on the impact of early nutritional status on the more disease‐susceptible midlife period. Here, we elucidate a moderately HP during critical development and the underlying mechanisms for liver function and metabolic health in middle‐aged offspring. Pregnant Wistar rats are given a 6‐week dietary intervention with a control diet and a moderately HP during gestation and lactation. The maternal high‐protein diet (mHP) group increases energy expenditure, preventing liver fat accumulation and alleviating systemic inflammation of middle‐aged offspring. Additionally, the mHP group reshapes offspring gut microbiota, significantly increasing the abundance of Clostridia_vadinBB60_group, Ruminococcaceae_UBA1819, Flavonifractor, and Butyricicoccaceae_UCG‐009. Metabolomics and transcriptomics reveal that mHP maintains energy homeostasis in middle‐aged offspring by altering serum amino acid, organic acid, and fatty acid profiles and activating the hepatic AMPK/SREBP‐1c pathway to preserve liver integrity via the gut–liver axis. We also demonstrate in the Drosophila model that mHP increases AMPK levels and alleviates lipid accumulation in the gut and fat body of middle‐aged flies. These findings provide valuable insights into the pivotal role of prenatal nutrition in offspring's long‐term health.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"66 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144532956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycyrrhizic Acid From Glycyrrhiza uralensis Fish: A Novel Therapeutic Candidate for Alzheimer's Disease Targeting the JNK Signaling Pathway. 从甘草鱼中提取的甘草酸：靶向JNK信号通路治疗阿尔茨海默病的新候选药物。

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-07-02 DOI: 10.1002/mnfr.70167

XiaoNa Song,XiaoTang Wang,Yao Gao,YaQi Liu,XiaoRu Yan,Zheng Xiang,Kuo Zhang,Zhao Fan,ZhaoYang Chen,GuoHua Song

{"title":"Glycyrrhizic Acid From Glycyrrhiza uralensis Fish: A Novel Therapeutic Candidate for Alzheimer's Disease Targeting the JNK Signaling Pathway.","authors":"XiaoNa Song,XiaoTang Wang,Yao Gao,YaQi Liu,XiaoRu Yan,Zheng Xiang,Kuo Zhang,Zhao Fan,ZhaoYang Chen,GuoHua Song","doi":"10.1002/mnfr.70167","DOIUrl":"https://doi.org/10.1002/mnfr.70167","url":null,"abstract":"Alzheimer's disease (AD) has emerged as a prevalent public health concern. This study aims to investigate the mechanism of Glycyrrhiza uralensis Fish. in AD through the utilization of network pharmacology, molecular docking, and in vitro validation techniques. Based on gene expression, a gene network was constructed to identify the core target genes related to Aβ and Tau, and the effective compounds of G. uralensis Fisch. were screened by contribution index (CI), which reflects the relative importance of each gene for Aβ/Tau. Molecular docking analysis revealed that glycyrrhizic acid (GA) strongly binds to these core targets. Next, an in vitro model of AD was established by inducing microglia with Aβ1-42. This model was used to assess the effects of GA on microglial apoptosis, migration, and inflammation. RT-qPCR (real time quantitative polymerase chain reaction) results indicated that GA lowered the expression of Tbk1 (TANK-binding kinase 1) and Soat1 (sterol O-acyltransferase 1) while simultaneously increasing the expression of Slc2a3 (solute carrier family 2 member 3). Additionally, Western Blot results suggested that GA inhibited the JNK signaling pathway. This demonstrates its anti-apoptotic and anti-inflammatory properties. Therefore, GA, through its modulation of key signaling pathways and gene expression, is expected to be a potential therapeutic candidate for AD. This study reveals that GA, a key compound in G. uralensis Fish, alleviates AD pathology by targeting Aβ/Tau-related genes (Tbk1, Soat1, and Slc2a3) and inhibiting the JNK pathway.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"16 1","pages":"e70167"},"PeriodicalIF":5.2,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144547930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bovine Milk Extracellular Vesicles as a Preventive Treatment for Bone Dysfunction and Metabolic Alterations in Obese Mice Fed a High‐Refined Carbohydrate Diet 牛乳细胞外囊泡作为高精制碳水化合物饮食对肥胖小鼠骨功能障碍和代谢改变的预防治疗

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-07-02 DOI: 10.1002/mnfr.70139

Francine R. F. Silva, Joyce E. Heredia, Onno J. Arntz, Breno R. Barrioni, Mauro M. Teixeira, Tarcília A. Silva, Fons A. J. van de Loo, Soraia Macari, Adaliene V. M. Ferreira, Marina C. Oliveira

{"title":"Bovine Milk Extracellular Vesicles as a Preventive Treatment for Bone Dysfunction and Metabolic Alterations in Obese Mice Fed a High‐Refined Carbohydrate Diet","authors":"Francine R. F. Silva, Joyce E. Heredia, Onno J. Arntz, Breno R. Barrioni, Mauro M. Teixeira, Tarcília A. Silva, Fons A. J. van de Loo, Soraia Macari, Adaliene V. M. Ferreira, Marina C. Oliveira","doi":"10.1002/mnfr.70139","DOIUrl":"https://doi.org/10.1002/mnfr.70139","url":null,"abstract":"Obesity may cause bone loss due to changes in energy and bone metabolism. Bone loss treatment is still limited, requiring new therapeutic strategies. Bovine milk extracellular vesicles (MEVs) are nanoparticles that act as modulators of cell signaling. While its benefits have already been demonstrated in bone loss, the underlying mechanisms must be better elucidated. To evaluate the effect of MEVs on bone loss in obesity, BALB/c mice were fed a chow diet or a high‐refined carbohydrate (HC) diet for 12 weeks and treated or not with MEVs from the 9th week. Mice fed the HC diet showed bone loss in the maxillary and long bones related to detrimental changes in the bone cell profile. As expected, the HC diet induced hyperglycemia and dyslipidemia, lipid accumulation in adipose and liver, and elevated receptor activator of nuclear factor kappa‐Β ligand (RANKL)/osteoprotegerin (OPG) ratio and Pentraxin 3 (PTX3) levels. MEV treatment protected from bone loss, increasing osteoblast and osteocyte numbers and reducing osteoclastic activity. Additionally, MEVs diminished adiposity, liver damage, serum glucose, triglyceride, and PTX3 levels, and the RANKL/OPG ratio. MEVs offer a protective effect against bone loss and improve metabolic outcomes in an HC diet‐induced obesity model, suggesting that metabolic improvements may contribute to their bone‐protective role.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"66 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144532996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal Dietary Fiber Intake During Lactation and Human Milk Oligosaccharide Fucosylation: a PRIMA Birth Cohort Study. 哺乳期间母亲膳食纤维摄入量和母乳低聚糖聚焦：一项PRIMA出生队列研究。

IF 4.5 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-06-30 DOI: 10.1002/mnfr.70165

Anneke H Hellinga, Samanta Cajic, Hanneke F Linde, Arthur H van Stigt, Jeanne H M de Vries, Elske M Brouwer-Brolsma, René Hennig, Erdmann Rapp, Marko Mank, Bernd Stahl, Aletta D Kraneveld, Jeanette H M Leusen, Louis Bont, Belinda Van't Land

{"title":"Maternal Dietary Fiber Intake During Lactation and Human Milk Oligosaccharide Fucosylation: a PRIMA Birth Cohort Study.","authors":"Anneke H Hellinga, Samanta Cajic, Hanneke F Linde, Arthur H van Stigt, Jeanne H M de Vries, Elske M Brouwer-Brolsma, René Hennig, Erdmann Rapp, Marko Mank, Bernd Stahl, Aletta D Kraneveld, Jeanette H M Leusen, Louis Bont, Belinda Van't Land","doi":"10.1002/mnfr.70165","DOIUrl":"https://doi.org/10.1002/mnfr.70165","url":null,"abstract":"Human milk oligosaccharides (HMOs) have an important role in the microbiome and immune system development of breastfed infants. Previous explorative studies indicated an association between maternal carbohydrate intake, including dietary fibers, and specific fucosylated HMOs in human milk (HM). Here, we aim to test whether the intake of dietary fibers by breastfeeding mothers is associated with the level of HMO-bound fucose in HM samples within a prospective birth cohort study. We assessed dietary fiber intake of healthy mothers (n = 164). HMO levels were semi-quantified in HM samples collected at 1 month postpartum. We found no correlation between fiber intake and HMO-bound fucose levels. However, secretor mothers (β = 2.22, p < 0.001) and mothers with a baby girl showed a positive correlation (β = 0.41, p = 0.016) with the level of HMO-bound fucose. In contrast, vaginal delivery negatively correlated with the level of HMO-bound fucose (β = -4.93, p = 0.008). Overall, there was no association between maternal fiber intake and HMO-bound fucose levels. Delivery mode, secretor status, and infant sex emerged as the dominant factors associated with HMO fucosylation in HM. Future research should investigate mechanisms underlying HMO fucosylation and its relevance for infant's health.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":" ","pages":"e70165"},"PeriodicalIF":4.5,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nicotinamide Riboside Supplementation Alleviates Heat Stress‐Induced Testicular Damage and Improves Semen Quality in Boars 添加烟酰胺核苷可减轻热应激诱导的公猪睾丸损伤和提高精液质量

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-06-30 DOI: 10.1002/mnfr.70162

Wenxue Shen, Xihao Luo, Xianyang Jin, Xiangyuan Ma, Xuemei Jiang, Chao Jin, Bin Feng, Lianqiang Che, Zhengfeng Fang, Shengyu Xu, Yan Lin, Yong Zhuo, De Wu, Lun Hua

{"title":"Nicotinamide Riboside Supplementation Alleviates Heat Stress‐Induced Testicular Damage and Improves Semen Quality in Boars","authors":"Wenxue Shen, Xihao Luo, Xianyang Jin, Xiangyuan Ma, Xuemei Jiang, Chao Jin, Bin Feng, Lianqiang Che, Zhengfeng Fang, Shengyu Xu, Yan Lin, Yong Zhuo, De Wu, Lun Hua","doi":"10.1002/mnfr.70162","DOIUrl":"https://doi.org/10.1002/mnfr.70162","url":null,"abstract":"Rising global temperatures and increasing extreme weather events have profound and widespread health impacts, particularly on vulnerable populations and sensitive organs. This study aimed to investigate the impact of HS on testicular NAD+ metabolism and assess the potential of NAD+ precursor supplementation in alleviating heat stress (HS)‐induced reductions in semen quality. This study aimed to investigate the impact of HS on testicular NAD+ metabolism and assess the potential of NAD+ precursor supplementation in alleviating HS‐induced reductions in semen quality. In this study, 24 Rongchang boars were randomly divided into three groups: a control group (CON), HS group, and HS supplementation with nicotinamide riboside group (HS‐NR). After the initial feeding phase, the boars were exposed to either a thermoneutral or HS environment for 2 weeks. In this study, we identified NAD+ deficiency as a hallmark of testicular damage under HS. Supplementation with NR effectively restored testicular NAD+ metabolism. NR supplementation also improved semen quality, reduced spermatogenesis defects, and attenuated oxidative stress and inflammation induced by HS. These findings highlight the potential of NAD+ precursors as a promising nutritional strategy to mitigate the adverse effects of HS on male reproductive health.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"35 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144515209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary Supplement With Tribulus terrestris L. Extract Exhibits Protective Effects on Neuroinflammation 蒺藜提取物对神经炎症的保护作用

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-06-30 DOI: 10.1002/mnfr.70006

Xiaohan Gao, Meng Li, Zhanbo Sun, Di Wu, Xiaohang Xu, Hongming Zhang, Zhen Yang, Shengxu Xie, Wenjun Guo, Ping Chen, Yajuan Xu

{"title":"Dietary Supplement With Tribulus terrestris L. Extract Exhibits Protective Effects on Neuroinflammation","authors":"Xiaohan Gao, Meng Li, Zhanbo Sun, Di Wu, Xiaohang Xu, Hongming Zhang, Zhen Yang, Shengxu Xie, Wenjun Guo, Ping Chen, Yajuan Xu","doi":"10.1002/mnfr.70006","DOIUrl":"https://doi.org/10.1002/mnfr.70006","url":null,"abstract":"Among herbal dietary supplements, an extract called Tribulus terrestris L. (TT) was widely used. We extracted an anti‐neuroinflammatory active fraction (TTAI) from TT and demonstrated that it is expected to be developed as a dietary supplement for ameliorating neuroinflammation through activity and mechanism exploration. The anti‐inflammatory activity of TT extract was evaluated by quantitative real‐time PCR (qRT‐PCR) and enzyme‐linked immunosorbent assay (ELISA). The data showed that TTAI significantly reduced the LPS‐induced increase in the levels of inflammatory factors in BV‐2 cells. In addition, TTAI significantly elevates the level of anti‐inflammatory factors. We applied metabolomics and transcriptomics to explore the pathways and targets underlying the therapeutic impact of TTAI in treating neuroinflammation. The results showed that TTAI could reverse the expression of 40 metabolic biomarkers that significantly changed in LPS‐induced BV‐2 inflammation models, including Cer, LPC, LPI, serine, lysin, and arginine, TTAI effectively regulated the expression levels of 235 genes, including Ptgs1, Pla2g7, and Plin2. Our data suggested that TTAI may function through PI3K/AKT and MAPK signaling pathways. This study uncovers the mechanisms behind the efficacy of TTAI in treating neuroinflammation, which not only confirms its potential as a dietary supplement, but also expands its potential medicinal applications.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"46 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144515210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Pomegranate Polyphenol Extract Suppresses the Microbial Production of Proatherogenic Trimethylamine (TMA) in an In Vitro Human Colon Model 石榴多酚提取物抑制体外人结肠模型中促动脉粥样硬化三甲胺（TMA）的微生物产生

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-06-30 DOI: 10.1002/mnfr.70166

Julia E. Haarhuis, Priscilla Day‐Walsh, Emad Shehata, George M. Savva, Barbora Peck, Mark Philo, Paul A. Kroon

{"title":"A Pomegranate Polyphenol Extract Suppresses the Microbial Production of Proatherogenic Trimethylamine (TMA) in an In Vitro Human Colon Model","authors":"Julia E. Haarhuis, Priscilla Day‐Walsh, Emad Shehata, George M. Savva, Barbora Peck, Mark Philo, Paul A. Kroon","doi":"10.1002/mnfr.70166","DOIUrl":"https://doi.org/10.1002/mnfr.70166","url":null,"abstract":"High circulating levels of trimethylamine N‐oxide (TMAO) are linked to metabolic diseases, adverse outcomes after heart failure, and atherogenic effects in animal models and in human subjects. l‐Carnitine and choline are major dietary precursors of TMAO. These are first converted to trimethylamine (TMA) by gut microbiota, which is absorbed by the host and converted into TMAO by hepatic flavin‐containing monooxygenases (FMOs). The minimal absorption of pomegranate polyphenols by the host suggests that they may reach the colon for further metabolism by the gut microbiome. This study investigates the ability of a polyphenol‐rich pomegranate extract to inhibit TMA production by human fecal microbiota. Batch fermentations were conducted with 1% human fecal inoculum, l‐carnitine, or choline, and a pomegranate extract (anaerobic, pH 6.6–7.1, 37°C) for 24 or 48 h. Methylamines were quantified using LC‐MS/MS with isotopically labeled internal standards. The pomegranate extract significantly delayed and reduced the rate of TMA production from both choline and l‐carnitine. The effect was dose‐dependent for l‐carnitine, with the highest dose delaying the average midpoint of l‐carnitine metabolism by 16 h (95% CI = 8.4‐24; p = 0.001). The pomegranate extract significantly reduced TMA production from choline and l‐carnitine in vitro.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"7 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144515211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0