Molecular Nutrition & Food Research最新文献

Investigation of Potential Food Intake Biomarkers by LC‐MS/MS: Suitability Under Conditions Close to Everyday Live

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2024-12-21 DOI: 10.1002/mnfr.202400575

Amelie Frank, Florian Hübner, Hans‐Ulrich Humpf

{"title":"Investigation of Potential Food Intake Biomarkers by LC‐MS/MS: Suitability Under Conditions Close to Everyday Live","authors":"Amelie Frank, Florian Hübner, Hans‐Ulrich Humpf","doi":"10.1002/mnfr.202400575","DOIUrl":"https://doi.org/10.1002/mnfr.202400575","url":null,"abstract":"ScopeFood intake biomarkers are proposed to be a more reliable tool for investigating the nutritional status compared to food diaries or food frequency questionnaires. Nevertheless, biomarkers have some disadvantages such as the influence of the individual lifestyle, different metabolism, nutrient–nutrient interaction or the specificity of the used biomarkers. To evaluate the suitability of several biomarkers, a study under conditions close to everyday life was conducted.Methods and resultsTen healthy volunteers were divided into three groups who followed different meal plans for 24 h and collected their entire urine. Afterward, several potential biomarkers were quantified using an LC‐MS/MS approach. To further investigate the suitability of those biomarkers, the results were investigated with the main goal of grouping the participants according to their provided diet, while the first author did not have any knowledge of the meal plans or other specific knowledge about the study design.ConclusionThe use of different biomarkers enables the evaluation of different eating habits without any prior wash out period or bigger than usual portion sizes. Especially long‐term biomarkers seem to have a good suitability. Very high intakes and very low intakes are easier to distinguish than a moderate intake. Food intake biomarkers are proposed as a more reliable tool than food diaries or food frequency questionnaires. Nevertheless, data under realistic conditions are rarely available. In this study, ten volunteers were divided into three groups with different meal plans. An LC‐MS/MS approach was performed and the data were further investigated with the main goal of grouping the participants according their provided diet, while the first author did not have any knowledge about the meal plans.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"31 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EPA/DHA but Not ALA Reduces Visceral Adiposity and Adipocyte Size in High Fat Diet-Induced Obese Delta-6 Desaturase Knockout Mice

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2024-12-20 DOI: 10.1002/mnfr.202400721

James N. Smorenburg, Katarzyna Hodun, Patrick V. McTavish, Chenxuan Wang, Marina A. Pinheiro, Kyle R. D. Wells, Keith R. Brunt, Manubu T. Nakamura, Adrian Chabowski, David M. Mutch

{"title":"EPA/DHA but Not ALA Reduces Visceral Adiposity and Adipocyte Size in High Fat Diet-Induced Obese Delta-6 Desaturase Knockout Mice","authors":"James N. Smorenburg, Katarzyna Hodun, Patrick V. McTavish, Chenxuan Wang, Marina A. Pinheiro, Kyle R. D. Wells, Keith R. Brunt, Manubu T. Nakamura, Adrian Chabowski, David M. Mutch","doi":"10.1002/mnfr.202400721","DOIUrl":"https://doi.org/10.1002/mnfr.202400721","url":null,"abstract":"The objective of this omega-3 feeding study was to elucidate the independent effects of α-linolenic acid (ALA) versus eicosapentaenoic (EPA)/docosahexaenoic acid (DHA) on visceral adiposity and inflammatory signaling in diet-induced obese delta-6 desaturase (Fads2) knockout (KO) mice. Male wildtype (WT) and Fads2 KO mice were fed a high-fat diet (45% kcal from fat) containing either lard (no omega-3s), flaxseed (ALA), or menhaden (EPA/DHA) for 21 weeks. Epididymal white adipose tissue (eWAT) was analyzed for changes in tissue weight, adipocyte size, triacylglycerol (TAG) and fatty acid content, and inflammatory markers. Despite no differences in final body weight, menhaden-fed mice had lower eWAT weight, smaller adipocytes, and lower TAG content compared to lard-fed mice regardless of Fads2 genotype. The eWAT of flaxseed-fed WT mice resembled menhaden-fed mice, while the eWAT of flaxseed-fed KO mice resembled lard-fed mice. No differences were observed in the expression of genes regulating eWAT inflammatory signaling (Tnfα, Nfκb, Mapk14, Mcp1, Ccl5, Tlr4, Nlrp3, or Adipoq) or the abundance of select proteins (p38-MAPK or MCP-1). In conclusion, a high-fat diet containing EPA/DHA, but not ALA, attenuates adipocyte hypertrophy and lowers TAG content but has no effect on eWAT inflammation in a mouse model of long-term diet-induced obesity.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"52 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible Involvement of Hippocampal miR-539-3p/Lrp6/Igf1r Axis for Diminished Working Memory in Mice Fed a Low-Carbohydrate and High-Protein Diet

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2024-12-20 DOI: 10.1002/mnfr.202400648

Takeru Shima, Hayate Onishi, Chiho Terashima

{"title":"Possible Involvement of Hippocampal miR-539-3p/Lrp6/Igf1r Axis for Diminished Working Memory in Mice Fed a Low-Carbohydrate and High-Protein Diet","authors":"Takeru Shima, Hayate Onishi, Chiho Terashima","doi":"10.1002/mnfr.202400648","DOIUrl":"https://doi.org/10.1002/mnfr.202400648","url":null,"abstract":"A low-carbohydrate and high-protein (LC-HP) diet demonstrates favorable impacts on metabolic parameters, albeit it leads to a decline in hippocampal function with the decreased expression of hippocampal insulin-like growth factor-1 receptor (IGF-1R) among healthy mice. However, the precise mechanisms underlying this phenomenon remain unexplored. Eight-week-old male C57BL/6 mice were divided into the LC-HP diet-fed group (25.1% carbohydrate, 57.2% protein, and 17.7% fat as percentages of calories; n = 10) and the control diet-fed group (58.9% carbohydrate, 24.0% protein, and 17.1% fat; n = 10). After 4 weeks, all mice underwent the Y-maze test, followed by analyses of hippocampal mRNA and miRNA expressions. We revealed that feeding the LC-HP diet suppressed working memory function and hippocampal Igf1r mRNA levels in mice. Sequencing of hippocampal miRNA demonstrated 17 upregulated and 27 downregulated miRNAs in the LC-HP diet-fed mice. Notably, we found decreased hippocampal mRNA levels of low-density lipoprotein receptor-related protein 6 (Lrp6), a gene modulated by miR-539-3p, in mice fed the LC-HP diet. Furthermore, a significant positive correlation was observed between Lrp6 and Igf1r mRNA levels in the hippocampus. These findings suggest that LC-HP diets may suppress hippocampal function via the miR-539-3p/Lrp6/Igf1r axis.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"14 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Neuroprotective Effect of Rooibos Herbal Tea Against Alzheimer's Disease: A Review

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2024-12-19 DOI: 10.1002/mnfr.202400670

Elias Chipofya, Taskeen F. Docrat, Jeanine L. Marnewick

{"title":"The Neuroprotective Effect of Rooibos Herbal Tea Against Alzheimer's Disease: A Review","authors":"Elias Chipofya, Taskeen F. Docrat, Jeanine L. Marnewick","doi":"10.1002/mnfr.202400670","DOIUrl":"https://doi.org/10.1002/mnfr.202400670","url":null,"abstract":"The world is experiencing a demographic shift toward an increasing proportion of elderly persons. Alzheimer's disease (AD) and other neurological disorders are far more likely to develop as people age. AD is a gradual, irreversible, and degenerative brain disorder that progressively deteriorates memory and cognitive function, eventually leading to death. Treatment for AD is the most significant unmet clinical need in neurology. There are no effective treatment options to prevent or reverse the degenerative process. The current medical management focuses primarily on temporarily easing symptoms, with little or no overall improvement. Although genetic predisposition and lifestyle factors influence the risk of neurodegenerative disorders, recent research suggests that dietary polyphenols with solid antioxidant capacities play crucial roles in determining brain health and aging. Aspalathus linearis is used to produce Rooibos, a popular South African herbal tea, which may modulate neurodegenerative mechanisms such as oxidative stress, tau protein, amyloid plaques, inflammation, and metals, all of which have been associated with AD. We reviewed the literature to evaluate the potential neuroprotective effects of Rooibos and its major flavonoids and to understand the underlying molecular mechanisms.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"23 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Mol. Nutr. Food Res. 23'24 发行信息：Mol.Nutr.Food Res.

IF 4.5 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2024-12-18 DOI: 10.1002/mnfr.202470036

引用次数: 0

Serum Lipidomic Signatures Mediate the Association Between Coarse Grain Preference and Central Obesity in Adults With Normal Weight and High Wheat Intake

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2024-12-18 DOI: 10.1002/mnfr.202400515

Kun Xu, Binyan Zhang, Yifei He, Yutong Wang, Yezhou Liu, Guoshuai Shi, Yuan Shen, Fangyao Chen, Baibing Mi, Lin Shi, Lingxia Zeng, Xin Liu, Shaonong Dang, Hong Yan

{"title":"Serum Lipidomic Signatures Mediate the Association Between Coarse Grain Preference and Central Obesity in Adults With Normal Weight and High Wheat Intake","authors":"Kun Xu, Binyan Zhang, Yifei He, Yutong Wang, Yezhou Liu, Guoshuai Shi, Yuan Shen, Fangyao Chen, Baibing Mi, Lin Shi, Lingxia Zeng, Xin Liu, Shaonong Dang, Hong Yan","doi":"10.1002/mnfr.202400515","DOIUrl":"https://doi.org/10.1002/mnfr.202400515","url":null,"abstract":"Little is known about the association between grain preference andabdominal fat accumulation, and mediating roles of circulating lipidomicsignatures. We quantified 1245 circulating lipids in 150 normal-weight centralobesity (NWCO) cases and 150 controls using targeted lipidomics. Grainpreference was determined by the highest intake frequency of grains (whiterice, wheat, or coarse grain). In our participants with high wheat intakefrequency, preferring coarse grain over rice was associated with a 60% lowerrisk of NWCO. Of the 585 lipids showing opposing associations with white riceand coarse grains, 46 were significantly linked to either (p < 0.05), predominantly alkylacyl phospholipids (PE-Os; n < 9) and alkenylacylphospholipids (PE-Ps; nx = 7). Network analysis identified a module primarilycomposed of PE-Os and PE-Ps, which was positively associated with coarse grain (p = 0.014). Another module, mainly consisting of triacylglycerols (TGs), was associatedwith white rice (p = 0.003) and mediated the association between white rice(mediation proportion: 20.30%; p = 0.027) or coarse grain preference (11.43%; p = 0.040) and NWCO. Specific lipids, such as TG(8:0_16:0_16:0) and TG(8:0_14:0_18:0), exhibited notable mediation effects. In normal-weight individuals with highwheat intake frequency, preferring coarse grain was inversely associated with NWCO, mediated by specific lipidomic signatures.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"496 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wolfberry Honey and Its Extract Alleviate Dextran Sodium Sulfate-Induced Ulcerative Colitis by Improving Intestinal Barrier Function and Reducing Oxidative Stress and Inflammation

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2024-12-17 DOI: 10.1002/mnfr.202400726

Jie Yuan, Qian Wu, Yuyang Guo, Yujing Tang, Shuangshuang Gao, Ting Li, Hongzhuan Xuan

{"title":"Wolfberry Honey and Its Extract Alleviate Dextran Sodium Sulfate-Induced Ulcerative Colitis by Improving Intestinal Barrier Function and Reducing Oxidative Stress and Inflammation","authors":"Jie Yuan, Qian Wu, Yuyang Guo, Yujing Tang, Shuangshuang Gao, Ting Li, Hongzhuan Xuan","doi":"10.1002/mnfr.202400726","DOIUrl":"https://doi.org/10.1002/mnfr.202400726","url":null,"abstract":"Inflammatory bowel disease (IBD) is a chronic condition characterized by gut inflammation causing persistent diarrhea and abdominal pain. Despite the nutritional benefits of wolfberry honey (from Lycium barbarum L.), its potential to alleviate IBD remains underexplored. This study evaluated the protective effects of wolfberry honey and its extract (wolfberry honey extract [WHE]) against dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) using in vivo and in vitro models. Mice pretreated with wolfberry honey showed significant symptom improvement in DSS-induced UC, linked to reduced expression of proinflammatory markers (Il-1β, Il-6, Tnf-α, and Mcp-1) and increased antioxidant genes (Nrf2, Sod2). Increased Occludin levels indicated improved intestinal barrier function. In vitro, WHE protected DSS-treated Caco-2 cells by lowering reactive oxygen species (ROS), stabilizing mitochondrial membrane potential, and inhibiting TLR4/NF-κB signaling. It enhanced the expression of antioxidant genes and tight junction proteins (ZO-1, Occludin, and Claudin-1). Metabolomic analysis revealed that WHE modulated glycerophospholipid metabolism, increasing phosphatidylcholine and choline levels and decreasing lysophosphatidylcholine levels. These results highlight the potential of wolfberry honey and its extract as nutraceuticals for managing UC through their effects on inflammation, oxidative stress, and intestinal barrier function. Further research is warranted to elucidate their mechanisms of action and assess their long-term therapeutic benefits in IBD management.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"64 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactobacillus acidophilus 6074 Fermented Jujube Juice Ameliorated DSS-induced Colitis via Repairing Intestinal Barrier, Modulating Inflammatory Factors, and Gut Microbiota

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2024-12-15 DOI: 10.1002/mnfr.202400568

Hongcai Li, Lingjia Fan, Siqi Yang, Pei Tan, Wenzhi Lei, Haihua Yang, Zhenpeng Gao

{"title":"Lactobacillus acidophilus 6074 Fermented Jujube Juice Ameliorated DSS-induced Colitis via Repairing Intestinal Barrier, Modulating Inflammatory Factors, and Gut Microbiota","authors":"Hongcai Li, Lingjia Fan, Siqi Yang, Pei Tan, Wenzhi Lei, Haihua Yang, Zhenpeng Gao","doi":"10.1002/mnfr.202400568","DOIUrl":"https://doi.org/10.1002/mnfr.202400568","url":null,"abstract":"Lactobacillus acidophilus L. acidophilus Lactobacillus, Bifidobacterium, and Akkermansia, This study aimed to explore the ameliorative effects and underlying mechanisms of oral administration Lactobacillus acidophilus 6074 fermented jujube juice (LAFJ) on dextran sulfate sodium (DSS)-induced colitis in mice. In this study, jujube juice was used as a substrate and fermented by L. acidophilus 6074 to investigate its effects on gut microbiota, intestinal barrier function, oxidative stress, inflammatory factors, and short-chain fatty acids (SCFAs) in mice with colitis and to reveal its potential mechanism for alleviating colitis. The results demonstrated that fermentation caused significant changes in the nutrients and nonnutrients of jujube juice, mainly in organic acids (malic acid, lactic acid, citric acid, and succinic acid) and free amino acids (Thr, Met, Ser, Ile, and Lys). High-dose LAFJ (20 mL/kg/day) significantly reduced the disease activity index (DAI), improved histopathological morphology, and increased colon length in colitis mice. LAFJ alleviated colon damage and preserved the integrity of the colonic mucosal barrier by promoting the expression of colonic tight junction proteins occludin, claudin-1, and zonula occluden-1 (ZO-1). Furthermore, LAFJ inhibited the production of proinflammatory factors and attenuated oxidative stress. Gut microbiota of mice revealed that LAFJ increased beneficial bacteria such as Lactobacillus, Bifidobacterium, and Akkermansia, promoted the production of SCFAs, and inhibited the growth of harmful microorganisms. Overall, LAFJ could reshape and restore gut microbiota imbalance caused by intestinal inflammation and alleviate the development of colitis, which may become a novel dietary intervention.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"16 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Basis and Molecular Mechanism of Aged Qingzhuan Tea Alleviating DSS-Induced Colitis

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2024-12-15 DOI: 10.1002/mnfr.202400734

Kang Wei, Qiurong Wei, Yang Wei, Lanlan Peng, Lizeng Cheng, Yuzhi Zhu, Yuanfeng Wang, Xinlin Wei

{"title":"Chemical Basis and Molecular Mechanism of Aged Qingzhuan Tea Alleviating DSS-Induced Colitis","authors":"Kang Wei, Qiurong Wei, Yang Wei, Lanlan Peng, Lizeng Cheng, Yuzhi Zhu, Yuanfeng Wang, Xinlin Wei","doi":"10.1002/mnfr.202400734","DOIUrl":"https://doi.org/10.1002/mnfr.202400734","url":null,"abstract":"Scope: Inflammatory bowel disease (IBD) poses a serious threat to human health. Qingzhuan tea (QZT), especially aged QZT, was concerned to have a potential effect on the prevention of colitis. In this study, we aim to assess the feasibility of different aged QZT on the alleviation of colitis induced by DSS. Methods and results: A comprehensive investigation into the efficacy of QZT of different aging years was conducted by establishing the animal model of colitis and the cellular inflammation model. The results demonstrated that QZT aged 0–20 years could significantly alleviate the symptoms of colitis. Notably, QZT aged for 5 years (A5) and 10 years (A10) was particularly effective in downregulating the levels of proinflammatory cytokines, via suppressing the activation of the NF-κB p65 pathway and upregulating the expression of the Nrf2/ARE pathway. The additional upregulation of gut microbiota including Allobaculum and Lactobacillus and superior alleviation on mitochondrial damage may be the mechanisms for A10 to show the better activity than A0 on alleviating colitis. Conclusion: Our study highlights the potential of QZT, especially A5 and A10, and provides valuable insights for the development of functional foods targeting colitis.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"38 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Optimized Ex Vivo n–3 PUFA Supplementation Strategy for Primary Human Macrophages Shows That DHA Suppresses Prostaglandin E2 Formation

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2024-12-15 DOI: 10.1002/mnfr.202400716

Rebecca Kirchhoff, Nadja Kampschulte, Carina Rothweiler, Nadine Rohwer, Karsten-Henrich Weylandt, Nils Helge Schebb

{"title":"An Optimized Ex Vivo n–3 PUFA Supplementation Strategy for Primary Human Macrophages Shows That DHA Suppresses Prostaglandin E2 Formation","authors":"Rebecca Kirchhoff, Nadja Kampschulte, Carina Rothweiler, Nadine Rohwer, Karsten-Henrich Weylandt, Nils Helge Schebb","doi":"10.1002/mnfr.202400716","DOIUrl":"https://doi.org/10.1002/mnfr.202400716","url":null,"abstract":"Evidence suggests beneficial effects of long-chain n–3 polyunsaturated fatty acids (PUFAs) in inflammatory diseases. However, the underlying mechanisms are still subject of research. For this purpose, we developed an ex vivo n–3 PUFA supplementation strategy. M2-like macrophages were supplemented for 2–3 days with 20–40 µM docosahexaenoic acid (DHA) during differentiation. Quality parameters include <3% oxylipins for PUFA-preparation, total fatty acids (FAs) <10 mM, and low oxylipins in plasma, n–3 PUFA <0.25 mM for the selection of donors of plasma as well as %n–6 in highly unsaturated fatty acids (HUFAs) ≥70% for donors of cells. Following supplementation, PUFA pattern of cells was shifted toward one described for blood and tissue from subjects with higher n–3 and lower n–6 PUFAs. This was accompanied by a decrease of arachidonic acid-derived oxylipins in a dose- and time-dependent manner in favor of n–3 PUFA ones. Stimulation with LPS resulted in decreased levels of pro-inflammatory prostaglandins in the DHA-supplemented cells, but no changes in cytokines. In vitro supplementation studies with n–3 PUFA need rigorous controls to exclude the background formation of oxylipins. By accounting for these possible confounders the described approach allows the mechanistic investigation of n–3 PUFAs in primary human immune cells, offering an alternative for intervention studies.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"6 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0