Molecular Nutrition & Food Research最新文献

Immunomodulatory Properties of Live and Thermally-Inactivated Food-Origin Lactic Acid Bacteria—In Vitro Studies

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-04-01 DOI: 10.1002/mnfr.70047

Wioletta Mosiej, Ewa Długosz, Marcin Kruk, Dorota Zielińska

{"title":"Immunomodulatory Properties of Live and Thermally-Inactivated Food-Origin Lactic Acid Bacteria—In Vitro Studies","authors":"Wioletta Mosiej, Ewa Długosz, Marcin Kruk, Dorota Zielińska","doi":"10.1002/mnfr.70047","DOIUrl":"https://doi.org/10.1002/mnfr.70047","url":null,"abstract":"The study investigates the strain-specific immunomodulatory properties of live and thermally-inactivated (TI) lactic acid bacteria (LAB) derived from traditional Polish fermented foods, focusing on their potential as probiotics and postbiotics. LAB strains, known for their role in food fermentation, were assessed for their ability to influence cytokine production in THP-1 macrophages, maintain intestinal epithelial barrier integrity in Caco-2 monolayers, exhibit antioxidant activity, and produce specific organic acids and sugars. The research demonstrated that live LAB strains significantly upregulated the anti-inflammatory cytokine IL-10, particularly under inflammatory conditions, while TI strains exhibited notable antioxidant and anti-inflammatory properties. TI strains showed a greater ability to protect epithelial barrier function and reduce pro-inflammatory cytokine secretion than live strains, suggesting a promising role for postbiotics. The findings underscore the potential of LAB from fermented foods, demonstrating that postbiotic derivatives can differently influence inflammation compared to live bacteria, highlighting their potential as immune-enhancing agents, capable of modulating immune responses and offering therapeutic benefits against inflammation-related disorders. However, the limitations of in vitro models highlight the need for further in vivo and clinical studies to validate these effects and fully uncover the health benefits of these LAB strains for humans.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"37 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Antiviral Activity of Polyphenols

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-04-01 DOI: 10.1002/mnfr.70042

Markus Burkard, Alban Piotrowsky, Christian Leischner, Katja Detert, Sascha Venturelli, Luigi Marongiu

{"title":"The Antiviral Activity of Polyphenols","authors":"Markus Burkard, Alban Piotrowsky, Christian Leischner, Katja Detert, Sascha Venturelli, Luigi Marongiu","doi":"10.1002/mnfr.70042","DOIUrl":"https://doi.org/10.1002/mnfr.70042","url":null,"abstract":"Polyphenols are secondary metabolites produced by a large variety of plants. These compounds that comprise the class of phenolic acids, stilbenes, lignans, coumarins, flavonoids, and tannins have a wide range of employment, from food production to medical usages. Among the beneficial applications of polyphenols, their antiviral activity is gaining importance due to the increased prevalence of drug-resistant viruses such as herpes and hepatitis B viruses. In the present review, we provide an overview of the most promising or commonly used antiviral polyphenols and their mechanisms of action focusing on their effects on enveloped viruses of clinical importance (double-stranded linear or partially double-stranded circular DNA viruses, negative sense single-stranded RNA viruses with nonsegmented or segmented genomes, and positive sense single-stranded RNA viruses). The present work emphasizes the relevance of polyphenols, in particular epigallocatechin-3-gallate and resveratrol, as alternative or supportive antivirals. Polyphenols could interfere with virtually all steps of viral infection, from the adsorption to the release of viral particles. The activity of polyphenols against viruses is especially relevant given the risk of widespread outbreaks associated with viruses, remarked by the recent COVID-19 pandemic.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"13 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Mol. Nutr. Food Res. 7'25

IF 4.5 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-04-01 DOI: 10.1002/mnfr.70036

引用次数: 0

Protective Effects of Flavonoids in Diabetic Cardiomyopathy: A Comprehensive Review on the Mechanistic Insights

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-31 DOI: 10.1002/mnfr.70038

Sonia Ramos

引用次数: 0

Protective Effects of Propolis Supplementation on Aflatoxin B1-Induced Oxidative Stress, Antioxidant Status, Intestinal Barrier Damage, and Gut Microbiota in Rats 补充蜂胶对黄曲霉毒素 B1 诱导的大鼠氧化应激、抗氧化状态、肠道屏障损伤和肠道微生物群的保护作用

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-30 DOI: 10.1002/mnfr.70052

Sevtap Kabalı, Mehtap Ünlü Söğüt, Neslihan Öner, Ayça Kara

{"title":"Protective Effects of Propolis Supplementation on Aflatoxin B1-Induced Oxidative Stress, Antioxidant Status, Intestinal Barrier Damage, and Gut Microbiota in Rats","authors":"Sevtap Kabalı, Mehtap Ünlü Söğüt, Neslihan Öner, Ayça Kara","doi":"10.1002/mnfr.70052","DOIUrl":"https://doi.org/10.1002/mnfr.70052","url":null,"abstract":"Aflatoxin B1 (AFB1) is common in the diets of humans and animals and often leads to adverse health effects. Propolis, with its strong antioxidant activity, can reduce oxidative stress and modulate gut microbiota composition. However, the underlying mechanism by which propolis alleviates AFB1-induced intestinal barrier damage remains unclear. This study was designed to investigate the protective effects of oral propolis supplementation in AFB1-exposed rats. Thirty-two male Sprague-Dawley rats were divided into four groups: control, AFB1, propolis, and AFB1+propolis. After 4 weeks, serum oxidative stress markers were examined, and gut microbiota was analyzed by 16S rRNA sequencing. Intestinal sections were processed by Hematoxylin & Eosin staining, and the expression level of tight junction proteins was assessed by immunostaining. Propolis supplementation in AFB1-exposed rats tended to decrease oxidative stress, and it also restructured the gut microbiota by preventing a decrease in the relative abundances of Lactobacillus, Roseburia, and Phascolarctobacterium. Propolis restored intestinal permeability impaired by AFB1 by ameliorating intestinal morphological damage and increasing the expression levels of tight junction proteins. Propolis supplementation may contribute to the modulation of gut microbiota by alleviating oxidative stress and improving intestinal barrier damage in AFB1-exposed rats.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"31 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunomodulatory Effects of Omega-3 Fatty Acids: Mechanistic Insights and Health Implications

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-30 DOI: 10.1002/mnfr.202400752

Mahmut Bodur, Birsen Yilmaz, Duygu Agagunduz, Yeşim Ozogul

{"title":"Immunomodulatory Effects of Omega-3 Fatty Acids: Mechanistic Insights and Health Implications","authors":"Mahmut Bodur, Birsen Yilmaz, Duygu Agagunduz, Yeşim Ozogul","doi":"10.1002/mnfr.202400752","DOIUrl":"https://doi.org/10.1002/mnfr.202400752","url":null,"abstract":"Omega-3 fatty acids play a significant role in immunomodulation, with nutrigenomic approaches highlighting their impact on gene expression related to immune responses. Research indicates that omega-3 fatty acids can modulate inflammatory pathways, potentially reducing chronic inflammation and enhancing immune function. This review discusses the intersection of nutrigenomics and nutriepigenomics, focusing on how omega-3 fatty acids influence gene expression, immune function, and overall health. The immune system is a complex network responsible for defending the body against pathogens and maintaining internal balance. Comprised of innate and adaptive immunity, the system involves various cells, tissues, and organs working together to combat infections and prevent diseases. Omega-3 polyunsaturated fatty acids (PUFAs), particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), play a significant role in modulating the immune system. These fatty acids influence immune cell function, membrane fluidity, and signaling processes, enhancing immune responses and reducing inflammation. Furthermore, EPA and DHA affect several signaling pathways, reducing the expression of proinflammatory cytokines and inhibiting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation, a critical transcription factor in the inflammatory response. Additionally, they activate PPAR-γ, further diminishing inflammatory gene expression. As precursors to specialized proresolving lipid mediators, EPA and DHA help shift the lipid mediator profile from proinflammatory to antiinflammatory derivatives, thus aiding in the resolution of inflammation.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"37 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Excess Fructose Intake Activates Hyperinsulinemia and Mitogenic MAPK Pathways in Association With Cellular Stress, Inflammation, and Apoptosis in the Pancreas of Rats

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-28 DOI: 10.1002/mnfr.70048

Ceren Guney, Mehmet Eray Alcigir, Fatma Akar

{"title":"Excess Fructose Intake Activates Hyperinsulinemia and Mitogenic MAPK Pathways in Association With Cellular Stress, Inflammation, and Apoptosis in the Pancreas of Rats","authors":"Ceren Guney, Mehmet Eray Alcigir, Fatma Akar","doi":"10.1002/mnfr.70048","DOIUrl":"https://doi.org/10.1002/mnfr.70048","url":null,"abstract":"The increase in sugar consumption has been associated with current metabolic disease epidemics. This study aimed to investigate the pancreatic molecular mechanisms involved in cellular stress, inflammation, mitogenesis, and apoptosis in metabolic disease induced by high-fructose diet. Here, we used biochemical, histopathological, Western blot, and immunohistochemistry methods to determine the metabolic and pancreatic alterations in male Wistar rats fed 20% fructose in drinking water for 15 weeks. High-fructose consumption in rats increased the immunopositivity and protein expression of glucose transporter 2 (GLUT2) and insulin in the pancreatic tissue, in association with abdominal adiposity, hyperglycemia, and hypertriglyceridemia. The expressions of cellular stress markers, glucose-regulated protein-78 (GRP78) and PTEN-induced putative kinase 1 (PINK1), were increased in the pancreas. The levels of interleukin (IL)-6, nuclear factor kappa B (NFκB), tumor necrosis factor α (TNFα), and IL-1β and components of the Nod-like receptor protein 3 (NLRP3) inflammasome were elevated. Excess fructose intake stimulated the activation of mitogenic extracellular signal-regulated kinases 1/2 (ERK1/2), p38, and c-Jun N-terminal kinase (JNK)1 as well as the apoptotic p53 and Fas pathways in the pancreas of rats. There was also an increase in caspase-8 and caspase-3 cleavage. Our findings revealed that dietary high-fructose in the pancreas causes hyperinsulinemia due to the upregulation of GLUT2 together with cellular stress and inflammatory markers, thereby stimulates mitogenic mitogen-activated protein kinase (MAPK) and apoptosis pathways, resulting in a complex pathological situation.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"30 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143723451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydroxytyrosol Enhances Plasma Extracellular Vesicle Secretion, Modulates Their miRNAs Cargo, and Reduces LDL Oxidation in Humans: Postprandial and 1-Week Effects

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-28 DOI: 10.1002/mnfr.70039

Andrea del Saz-Lara, María-Carmen López de las Hazas, Joao Tomé-Carneiro, Maria-Ángeles Ávila-Gálvez, María Carmen Crespo, Carmen Mazarío-Gárgoles, Luis A. Chapado, Lidia Daimiel, Francesco Visioli, Victoria Martín-Santiago, Juan Carlos Espín, Alberto Dávalos

{"title":"Hydroxytyrosol Enhances Plasma Extracellular Vesicle Secretion, Modulates Their miRNAs Cargo, and Reduces LDL Oxidation in Humans: Postprandial and 1-Week Effects","authors":"Andrea del Saz-Lara, María-Carmen López de las Hazas, Joao Tomé-Carneiro, Maria-Ángeles Ávila-Gálvez, María Carmen Crespo, Carmen Mazarío-Gárgoles, Luis A. Chapado, Lidia Daimiel, Francesco Visioli, Victoria Martín-Santiago, Juan Carlos Espín, Alberto Dávalos","doi":"10.1002/mnfr.70039","DOIUrl":"https://doi.org/10.1002/mnfr.70039","url":null,"abstract":"Hydroxytyrosol (HT) consumption is associated with healthy outcomes. Specifically, its ability to modulate microRNA (miRNA) expression has been described. However, whether HT influences the secretion of extracellular vesicles (EVs) and their transported miRNAs remains unknown. Here, following HT administration, we explored the potential changes in EV secretion and in their miRNA cargo during postprandial state and after 1 week of daily intake. Twelve healthy participants received, first, a single 25 mg dose of HT (postprandial study) and then 25 mg HT daily, for one week (sustained study). Plasma and circulating plasma-isolated EVs were analyzed for HT and derived metabolites using UPLC-qTOF-MS and miRNAs by RNA-seq. HT induced the secretion of EVs 1-h post-ingestion and a 1-week daily administration of HT increased fasting EVs and reduced circulating oxidized LDL levels. RNA-seq analysis identified 55 modulated miRNAs (30 upregulated and 25 downregulated) related to response to oxygen level and gland development, following 1-week HT supplementation. This pilot study sheds light on the effects of HT consumption on EV secretion and modulation of exosomal miRNAs in humans, which has been scarcely studied to date. Further research is warranted to elucidate the molecular mechanisms underlying the observed effects.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"23 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143723453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the Mechanism of Galangin on Hyperuricemic Nephropathy Based on Metabolomics and Network Pharmacology

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-27 DOI: 10.1002/mnfr.70029

Daermu Qumu, Mu Tian, Hengxi Li, Xiujuan Yang, Binhui Han, Lanting Wei, Bo Li, Mengxue Ma, Junjie He, Xiaoni Shao

{"title":"Study on the Mechanism of Galangin on Hyperuricemic Nephropathy Based on Metabolomics and Network Pharmacology","authors":"Daermu Qumu, Mu Tian, Hengxi Li, Xiujuan Yang, Binhui Han, Lanting Wei, Bo Li, Mengxue Ma, Junjie He, Xiaoni Shao","doi":"10.1002/mnfr.70029","DOIUrl":"https://doi.org/10.1002/mnfr.70029","url":null,"abstract":"Galangin (GAL), a flavonol found in Alpinia officinarum and propolis, is a promising functional food. This study investigated the therapeutic effects and mechanisms of GAL in mice with hyperuricemic nephropathy (HN) by focusing on renal metabolomics and network pharmacology. In this study, we conducted untargeted metabolomic analysis and network pharmacology prediction. Subsequently, a compound-reaction-enzyme-gene network was constructed based on the results of metabolomics and network pharmacology to elucidate potential connections. The results demonstrated that GAL can improve renal interstitial fibrosis and inflammatory infiltration and reduce serum levels of uric acid (UA), urea nitrogen (UREA), and creatinine (CREA). Metabolome analysis indicated that GAL affected thiamine, pyrimidine, nicotinate, nicotinamide, pyruvate, glyoxylate, and dicarboxylate metabolism. Network pharmacology and experimental results showed that GAL reduced the key target expression of the tumor protein P53 (TP53), tumor necrosis factor (TNF), signal transducer and activator of transcription 3 (STAT3), heat shock protein 90 alpha family class A member 1 (HSP90aa1), albumin (ALB), and caspase-3 (CASP3). GAL also downregulated the expression of Janus kinase 2 (JAK2), phospho-JAK2 (P-JAK2), and phospho-STAT3 (P-STAT3). Furthermore, a joint analysis of the metabolome and network pharmacology showed that GAL can reverse HN through amino acid metabolism, nucleotide metabolism, energy metabolism, and endocrine system pathways. GAL can alleviate HN effectively and might play synergistic therapeutic roles through regulating metabolic profiles and the JAK2/STAT3 signaling pathway.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"31 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143723463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apigenin Alleviates Intestinal Ischemia/Reperfusion Injury via Upregulating Nrf2-Mediated Tight Junction Integrity

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-27 DOI: 10.1002/mnfr.70043

Bin Xu, Yan Zhuang, Ying Zhang, Suoning Liu, Rongjun Fan, Weiru Jiang

{"title":"Apigenin Alleviates Intestinal Ischemia/Reperfusion Injury via Upregulating Nrf2-Mediated Tight Junction Integrity","authors":"Bin Xu, Yan Zhuang, Ying Zhang, Suoning Liu, Rongjun Fan, Weiru Jiang","doi":"10.1002/mnfr.70043","DOIUrl":"https://doi.org/10.1002/mnfr.70043","url":null,"abstract":"Epithelial barrier dysfunction, critically involved in intestinal ischemia/reperfusion (I/R) injury, is significantly regulated by Nrf2-mediated oxidative stress. Apigenin, a flavonoid commonly found in fruits and vegetables with diverse biological properties, has an unclear impact on intestinal I/R injury. We hypothesize that apigenin improves intestinal barrier dysfunction by activating Nrf2 signaling. Thirty rats were randomly divided into five groups to establish an I/R model using superior mesenteric artery occlusion. Hypoxia and re-oxygenation (H/R) model was developed utilizing Caco-2 and IEC-6 cells, which were exposed to hypoxic conditions followed by re-oxygenation. Apigenin protected against intestinal mucosal damage by suppressing inflammatory cytokines release (TNF-α, IL-1β, IL-6, MPO, p < 0.01), ameliorating oxidative stress (MDA, SOD, GSH, GSH-Px, p < 0.01), and improving barrier dysfunction (DAO and TEER, p < 0.01) both in vivo and in vitro, without causing significant changes in the corresponding normal controls (p > 0.05). Apigenin up-regulated the protein expression of Nrf2, HO-1, and tight junction (TJ) proteins (p < 0.01). Furthermore, the knockdown of Nrf2 significantly abrogated apigenin-enhanced the TJ expression. Apigenin pretreatment alleviates intestinal I/R-induced barrier damage through Nrf2 activation and TJ upregulation, offering new strategies for preventing or treating I/R-associated intestinal diseases.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"41 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143723452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0