Dietary Supplement With Tribulus terrestris L. Extract Exhibits Protective Effects on Neuroinflammation

IF 4.5 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Xiaohan Gao, Meng Li, Zhanbo Sun, Di Wu, Xiaohang Xu, Hongming Zhang, Zhen Yang, Shengxu Xie, Wenjun Guo, Ping Chen, Yajuan Xu
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引用次数: 0

Abstract

Among herbal dietary supplements, an extract called Tribulus terrestris L. (TT) was widely used. We extracted an anti‐neuroinflammatory active fraction (TTAI) from TT and demonstrated that it is expected to be developed as a dietary supplement for ameliorating neuroinflammation through activity and mechanism exploration. The anti‐inflammatory activity of TT extract was evaluated by quantitative real‐time PCR (qRT‐PCR) and enzyme‐linked immunosorbent assay (ELISA). The data showed that TTAI significantly reduced the LPS‐induced increase in the levels of inflammatory factors in BV‐2 cells. In addition, TTAI significantly elevates the level of anti‐inflammatory factors. We applied metabolomics and transcriptomics to explore the pathways and targets underlying the therapeutic impact of TTAI in treating neuroinflammation. The results showed that TTAI could reverse the expression of 40 metabolic biomarkers that significantly changed in LPS‐induced BV‐2 inflammation models, including Cer, LPC, LPI, serine, lysin, and arginine, TTAI effectively regulated the expression levels of 235 genes, including Ptgs1, Pla2g7, and Plin2. Our data suggested that TTAI may function through PI3K/AKT and MAPK signaling pathways. This study uncovers the mechanisms behind the efficacy of TTAI in treating neuroinflammation, which not only confirms its potential as a dietary supplement, but also expands its potential medicinal applications.
蒺藜提取物对神经炎症的保护作用
在草药膳食补充剂中,一种名为蒺藜的提取物(TT)被广泛使用。我们从TT中提取了一种抗神经炎症活性组分(TTAI),并通过活性和机制探索证明它有望被开发为一种改善神经炎症的膳食补充剂。采用定量实时PCR (qRT - PCR)和酶联免疫吸附试验(ELISA)评价TT提取物的抗炎活性。数据显示,TTAI可显著降低LPS诱导的BV - 2细胞炎症因子水平的升高。此外,TTAI可显著提高抗炎因子水平。我们应用代谢组学和转录组学来探索TTAI治疗神经炎症的途径和靶点。结果表明,在LPS诱导的BV - 2炎症模型中,TTAI可以逆转40种代谢生物标志物的表达,包括Cer、LPC、LPI、丝氨酸、lysin和精氨酸,TTAI可以有效调节235个基因的表达水平,包括Ptgs1、Pla2g7和Plin2。我们的数据表明,TTAI可能通过PI3K/AKT和MAPK信号通路起作用。本研究揭示了tai治疗神经炎症的作用机制,不仅证实了其作为膳食补充剂的潜力,而且扩大了其潜在的医学应用。
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来源期刊
Molecular Nutrition & Food Research
Molecular Nutrition & Food Research 工程技术-食品科技
CiteScore
8.70
自引率
1.90%
发文量
250
审稿时长
1.7 months
期刊介绍: Molecular Nutrition & Food Research is a primary research journal devoted to health, safety and all aspects of molecular nutrition such as nutritional biochemistry, nutrigenomics and metabolomics aiming to link the information arising from related disciplines: Bioactivity: Nutritional and medical effects of food constituents including bioavailability and kinetics. Immunology: Understanding the interactions of food and the immune system. Microbiology: Food spoilage, food pathogens, chemical and physical approaches of fermented foods and novel microbial processes. Chemistry: Isolation and analysis of bioactive food ingredients while considering environmental aspects.
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