Animal Bioscience最新文献

{"title":"Heated drinking water in winter improves growth performance of male Hu sheep by modulating rumen quorum sensing and metabolites, and enhancing serum antioxidant capacity.","authors":"Chang Liu, Lingyan Li, Jiaqi Dai, Mingren Qu, Kehui Ouyang, Qinghua Qiu","doi":"10.5713/ab.24.0821","DOIUrl":"10.5713/ab.24.0821","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to explore the mechanism by which increasing the temperature of drinking water in winter promotes sheep growth from a microbiological perspective.</p><p><strong>Methods: </strong>A total of 12 healthy male Hu sheep were evenly divided into two groups: one with drinking water at 12°C (WT12) and the other at 25°C (WT25), and they were raised for 60 days in the cold winter.</p><p><strong>Results: </strong>The WT25 group had higher average daily gain, serum immunoglobulin G, total antioxidant capacity, glutathione peroxidase, and superoxide dismutase, along with lower feed-to-gain ratio, serum cortisol, malondialdehyde, reactive oxygen species, and oxidative stress index when compared to the WT12 group (p<0.05). The concentrations of microbial crude protein, microbial density, autoinducer-2 signaling molecule concentration, and biofilm formation were higher in the WT25 group, while the ammonia nitrogen concentration was lower (p<0.05). The relative abundances of Muribaculum and Clostridia UCG-014, as well as the predicted metabolic pathways related to lipid metabolism, were lower in the WT25 group, whereas the metabolism of other amino acids showed increased abundances (p<0.05). Both principal coordinates analysis and analysis of similarities revealed no significant differences in rumen microbial communities between the WT12 and WT25 groups (p>0.05). Metabolomics analysis identified 12 differential metabolites, four of which were correlated with Muribaculum, Raoultibacter, and Coriobacteriales Incertae Sedis.</p><p><strong>Conclusion: </strong>These findings suggest that heated drinking water in winter may improve growth performance by increasing rumen microbial biofilm formation and enhancing serum antioxidant capacity in Hu sheep. This study reveals links between rumen microbial quorum sensing and critical parameters such as animal growth phenotypes, rumen metabolic characteristics, and specific bacterial genera. It offers innovative perspectives on enhancing animal feed efficiency through the modulation of rumen microbial quorum sensing.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2280-2296"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Mongolian Bergenia crassifolia L. (root) extract on rumen methane emission and microbial community.","authors":"Xinrui Zhao, Otgonpurev Sukhbaatar, Linlin Kou, Xinming Cheng, Metha Wanapat, Mahmoud Kamal, Togtokhbayar Norovsambuu, Zhanying Sun, Yanfen Cheng","doi":"10.5713/ab.24.0836","DOIUrl":"10.5713/ab.24.0836","url":null,"abstract":"<p><strong>Objective: </strong>The study aims to research the effects of Mongolian plants on reducing methane emissions, fermentation parameters, and microbial communities in grazing systems.</p><p><strong>Methods: </strong>Initially, various Mongolian plants were screened to assess their ability to reduce methane production in an in vitro experiment. The most effective plant was selected for extracting active components, and their concentrations were determined. In vitro rumen fermentation used rice straw and alfalfa as substrates. Extract were added at 0%, 1%, 2%, 3%, 4%, and 5% of dry matter. Measured parameters included gas production, methane production, and rumen fermentation parameters. After the fermentation experiments, we extracted DNA from the rumen fluid for quantitative PCR and 16S high-throughput sequencing analysis.</p><p><strong>Results: </strong>Bergenia crassifolia L. exhibited the most significant methane reduction, its root extract containing approximately 70% condensed tannin, decreased methane production in the rice straw substrate (p<0.01). The 5% addition also showed notable effcacy in the alfalfa substrate (p<0.01). Furthermore, 1% addition of the extract had no significant effect on rumen fermentation parameters. Ruminococcus and Christensenellaceae R-7 were emerged as key methane-reducing taxa (p<0.01).</p><p><strong>Conclusion: </strong>Bergenia crassifolia L. (root) extract demonstrated stronger methane reduction efficacy in low-quality roughage compared to high-quality roughage, with 1% being the optimal dose. This finding suggests Bergenia crassifolia L. potential as a new strategy for sustainable rumen methane mitigation in grazing systems.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2155-2172"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of yam bean pulp on growth performance, gut morphology, digestive development, and digestibility in broilers raised in hot environments.","authors":"Kedsirin Sakwiwatkul, Wannisa Ojan, Purinut Treehera, Jessada Pidtathasa, Theeranon Pomwong, Anut Chantiratikul, Siriporn Lawan, Manisa Sangkaew","doi":"10.5713/ab.25.0057","DOIUrl":"10.5713/ab.25.0057","url":null,"abstract":"<p><strong>Objective: </strong>Rearing broilers in high-temperature environments can impair growth performance, affecting the economic efficiency of broiler production. Dietary fiber sources like yam bean pulp (YBP) may mitigate these effects by promoting digestive organ development and gut integrity, enhancing nutrient absorption and growth. This study aimed to evaluate the impact of YBP inclusion on digestive development, gut morphology, nutrient digestibility, serum lipid profiles, and growth performance in broilers raised under high environmental temperatures.</p><p><strong>Methods: </strong>Since YBP has not previously been used in poultry diets, its optimal inclusion level was determined using in vitro digestibility measurements, which identified 4% YBP as the optimal initial inclusion level for further in vivo study. Subsequently, the in vivo study was conducted with 200 one-day-old ROSS broiler chicks, randomly assigned to four dietary treatments (0%, 4%, 8%, and 12% YBP; 5 replicates of 10 birds each), and housed in an open system at an average temperature of 32.1±3.7°C. Digestive traits, nutrient digestibility, serum lipid profiles, and growth performance were assessed over 21 days.</p><p><strong>Results: </strong>Results showed that YBP inclusion (up to 12%) improved gut morphology by increasing villi height and the villi height-to-crypt depth ratio in the duodenum and ileum, alongside dose-dependent improvements in dry matter digestibility. However, crude protein digestibility declined when YBP levels exceeded 8%. Despite these benefits, YBP inclusion did not alleviate heat stress effects on growth performance, digestive organ development, or serum lipid profiles, even at the highest inclusion level.</p><p><strong>Conclusion: </strong>Dietary YBP can enhance gut morphology and nutrient digestibility in broilers from day 1 to 21, with optimal inclusion levels not exceeding 8%. However, its use does not mitigate the negative effects of heat stress on broiler growth in high-temperature environments.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2203-2214"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of combined cryoprotectants on the cryotolerance of boar sperm.","authors":"Shuangyi Deng, Liwei Yang, Li Gao, Chengcheng Ning, Shiyin Wang, Wei Zhang","doi":"10.5713/ab.24.0915","DOIUrl":"10.5713/ab.24.0915","url":null,"abstract":"<p><strong>Objective: </strong>The frozen semen has the significant advantages of long-term storage and long-range transportation. However, due to the low cryotolerance of boar sperm, the global utilization of frozen boar semen in artificial insemination was less than 1% until the year 2000.</p><p><strong>Methods: </strong>In this study, the effects of five cryoprotectants at different concentrations on the cryotolerance of boar semen were evaluated when they were added separately, and the optimal concentrations for each cryoprotectant were determined, then their combined additive effects were further assessed.</p><p><strong>Results: </strong>At a glycerol (GLY) concentration of 5%, the quality of frozen-thawed sperm reached its maximum value, which was significantly higher than the 4% GLY group (p<0.05) and 0% GLY group (p<0.01). The straight-line velocity (VSL), curvilinear velocity (VCL), average path velocity (VAP), sperm plasma membrane integrity (SPMI), sperm acrosome integrity (SAI) and sperm mitochondrial activity (SMA) of the frozen-thawed sperm in treated egg yolk group exhibited significant improvements compared to the untreated egg yolk group (p<0.05). The total motility (TM), progressive motility (PM), SPMI, SAI, and SMA of 2% Equex STM paste group were significantly higher than the rest groups (p<0.05). The TM, PM, VSL, VCL, and VAP of frozen-thawed sperm in the 250 nM and 300 nM Mitoquinone mesylate groups showed significant improvements compared to the other groups (p<0.05), and the reactive oxygen species levels in sperm cells were also significantly lower (p<0.05). The quality of frozen-thawed boar sperm in 0.6 mM L-ergothioneine group reached its peak value and was significantly higher than the rest groups (p<0.05). When these five cryoprotectants were used in combination, the quality of frozen-thawed boar sperm exhibited a significant improvement compared to when they were used individually (p<0.05). Utilizing the frozen-thawed boar semen to inseminate estrus sows, the reproductive performance of the sows did not differ significantly from the sows inseminated with fresh semen (p>0.05).</p><p><strong>Conclusion: </strong>The optimized boar semen cryopreservation system can substantially enhance the quality of frozen-thawed boar sperm, making it suitable for artificial insemination in pig farm.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2111-2124"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating effect of different dominance genotype encodings on genome-wide association studies and genomic selection.","authors":"Xiangyu Dai, Jiakun Qiao, Zhiwei Long, Zhaoxuan Che, Fangjun Xu, Na Miao, Mengjin Zhu","doi":"10.5713/ab.24.0658","DOIUrl":"10.5713/ab.24.0658","url":null,"abstract":"<p><strong>Objective: </strong>The quantification of dominance effects varies across different models, and the appropriate coding in genetic analyses remains debated. This study investigated several proposed dominance encoding methods, evaluating their performance in genetic analyses.</p><p><strong>Methods: </strong>Three datasets, each representing the breeds Duroc, Landrace, and Yorkshire, were used in this study. We assessed heritability, genetic effects, and prediction accuracy in genomic selection (GS), as well as significant loci and statistical power in genome-wide association studies (GWAS).</p><p><strong>Results: </strong>In GS, correlations among additive effects and among total genetic effects across models were high (0.9 to 1) under different dominance encodings for most traits, while only the (0, 1, 0) and (0, 2p, 4p-2) encodings maintained high correlations for all traits. The average prediction accuracy of the additive-dominance model with the (0, 1, 0) encoding increased by 2.79% and 1.69%, respectively, compared to the (0, 1, 1) and (0, 2p, 4p-2) encodings for all traits. In GWAS, the (0, 1, 0) encoding had higher statistical power compared to the (0, 1, 1) and (0, 2p, 4p-2) encodings, especially for rare variants. Additionally, different dominance encodings identified independent and distinct significant loci.</p><p><strong>Conclusion: </strong>The (0, 1, 0) encoding method generally outperforms the others in genetic analyses, while alternative encodings provide complementary insights into dominance effects. These findings provide valuable guidance for selecting dominance encodings in genetic analyses.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2067-2078"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated metabolome and immunity analysis of immune-physiological responses in dairy cows under heat stress condition.","authors":"Jun Sik Eom, Sangjin Lee, Joonpyo Oh, Byeong Cheol Ban, Yeeun Kim, Goeun Han, Bon-Hee Gu, Eun-Tae Kim, Sang-Bum Kim, Sung Sill Lee, Myunghoo Kim","doi":"10.5713/ab.25.0038","DOIUrl":"10.5713/ab.25.0038","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the metabolic changes and immune responses in Jersey dairy cows under heat stress (HS). The focus was on understanding HS-induced alterations in metabolism and immunity compared to HS-free cows.</p><p><strong>Methods: </strong>Proton nuclear magnetic resonance spectroscopy-based metabolomics was performed on rumen fluid, serum, and milk samples which were collected through single sampling from HS-free (n = 9) and HS-exposed (n = 8) Jersey cows. Immune cell proportions and functions in peripheral blood mononuclear cells (PBMC) were analyzed to identify heat-sensitive immunological changes. Correlation analysis was conducted to link immune indicators with serum metabolites.</p><p><strong>Results: </strong>Metabolomics revealed potential HS biomarkers in biofluids: nicotinate and phenylacetate in rumen fluid; isopropanol in serum; and glycine and trehalose in milk. HS increased B cell and CD4+ T cell populations, as well as Th17 cells and IL-17A expression in PBMCs. Twenty-three metabolites correlated strongly with five immune indicators, with nine metabolites exhibiting a positive correlation and fourteen a negative correlation.</p><p><strong>Conclusion: </strong>HS alters the both metabolism and immune cell function in Jersey dairy cows. These findings provide key information for the development of diagnostic methods for HS and nutritional intervention strategies to mitigate HS for dairy cows.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2215-2232"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive meta-analysis on the efficacy of growth factor enriched cryo-agents in enhancing post-thaw quality of mammalian semen.","authors":"Suyatno Suyatno, Herdis Herdis, Tri Puji Priyatno, Mulyoto Pangestu, Santoso Santoso, Tatan Kostaman, Mohammad Firdaus Hudaya, Pangda Sopha Sushadi, Florentina Bety Indah Lupitasari, Anita Hafid, Zultinur Muttaqin, Ita Margaretha Nainggolan, Mohammad Miftakhus Sholikin, Pradita Iustitia Sitaresmi","doi":"10.5713/ab.24.0898","DOIUrl":"10.5713/ab.24.0898","url":null,"abstract":"<p><strong>Objective: </strong>This study sought to evaluate the effect of growth factors (GFs) in semen extenders on the quality of post-thaw mammalian sperm using a meta-analysis approach. The main objective was to determine whether the addition of GFs could improve semen quality after cryopreservation.</p><p><strong>Methods: </strong>A meta-analysis of various in vitro experiments using mammalian semen was conducted. Data were collected from multiple studies assessing the effects of GFs on sperm motility, viability, acrosome integrity, DNA integrity, and other key semen quality metrics. The analysis included a range of mammalian species, incorporating specific GFs into semen extenders during cryopreservation. The evaluation of sperm quality was conducted using parameters including motility, viability, acrosome integrity, plasma membrane integrity (PMI), DNA integrity, hyper-osmotic swelling test (HOST), malondialdehid (MDA), and computer-assisted semen analysis (CASA). Statistical analyses, including standardized mean differences (SMD), were performed to compare the effects of GF additives with control treatments.</p><p><strong>Results: </strong>The addition of GFs into semen extenders significantly improved semen quality across various parameters, including motility, viability, acrosome integrity, and DNA integrity. The SMDs for motility, viability, acrosome integrity, PMI, DNA integrity, HOST, MDA, and CASA parameters were significantly higher in the GF-treated groups than those of the controls, with observed values of 2.56±0.303, 3.53±0.423, 1.22±0.351, 1.82±0.362, 8.73±2.514, 2.02±0.426, and 6.30±2.87, respectively. Notably, the addition of GFs maintained semen quality in most mammalian species (p<0.05, SMD>0.5), with the exception of boar semen.</p><p><strong>Conclusion: </strong>The present study demonstrated that the addition of GFs into semen extenders significantly enhances semen quality during cryopreservation across various mammalian species. This improvement is likely due to the antioxidants and repair factors found in the GFs. Each GF appeared to exert a distinct effect on sperm, subsequently enhancing sperm viability after thawing. The findings have important implications for improving reproductive technologies in mammalian species, particularly regarding cryopreservation and artificial insemination procedures.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2094-2110"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of endogenous calcium loss from different sources in 45-week-old Hy-Line Brown laying hens.","authors":"Nuwan Chamara Chathuranga, Shan Randima Nawarathne, Elijah Ogola Oketch, Venuste Maniraguha, Bernadette Gerpacio Sta Cruz, Jeseok Lee, Haeeun Park, Hyunji Choi, Myunghwan Yu, Jung Min Heo","doi":"10.5713/ab.25.0008","DOIUrl":"10.5713/ab.25.0008","url":null,"abstract":"<p><strong>Objective: </strong>The study assessed the calcium (Ca) endogenous losses and digestibility in 45-week-old Hy-Line Brown laying hens fed diets incorporating different Ca-sourcing ingredients.</p><p><strong>Methods: </strong>A total of 168 hens were randomly assigned to dietary treatments, with six replicates, and four hens were housed per cage. The seven diets included different Ca sources of monocalcium phosphate (MCP), dicalcium phosphate (DCP), limestone, corn, soybean, wheat bran, and a Ca-free diet. All diets included 0.3% Cr2O3 as a digestible marker as well. Hens were given ad-libitum access to feed and water. On day 3, fresh excreta and the ileal digesta were collected to analyze dry matter, Ca, and Cr2O3 for digestibility analysis.</p><p><strong>Results: </strong>The Ca-free diets led to lower endogenous Ca losses at the ileum (p<0.05) compared to the Ca-supplemented groups. Among the Ca-sourcing diets, MCP resulted in higher Ca losses, whereas corn-based diets showed relatively lower losses at the ileum. Calcium losses at the excretory site were not significant, although the ileal losses were markedly higher (p<0.001), with diet-by-site interaction (p<0.05). Apparent and standardized ileal Ca digestibility were higher for inorganic sources (MCP, DCP, limestone) than for plant-based sources, with DCP showing greater digestibility (p<0.05).</p><p><strong>Conclusion: </strong>Inorganic Ca sources resulted in increased endogenous Ca loss and digestibility compared to plant seed-based layer diets, with these losses varying depending on the specific measurement sites.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2196-2202"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of varying levels of coated cysteamine hydrochloride in diet on growth performance and carcass quality of steers.","authors":"Watcharawit Meenongyai, Kannika Wongpanit, Pichad Khejornsart, Piyamas Phongkaew, Unchan Traithilen, Naroon Waramit, Md Maksudul Haque Helali, Alimul Islam Shimul, Abdullah Al Mamun","doi":"10.5713/ab.24.0863","DOIUrl":"10.5713/ab.24.0863","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the effects of different levels of coated cysteamine HCl (CSH) supplementation on growth performance, carcass traits, and meat quality in Charolais crossbred cattle.</p><p><strong>Methods: </strong>Twenty-four Charolais crossbred steers, aged 24-30 months with an initial body weight of 418±31 kg, were assigned to three dietary treatments: 0% (control), 0.5%, or 1.0% CSH in concentrate. The steers were fed the experimental diets for 200 days.</p><p><strong>Results: </strong>Increased CSH levels in concentrate diets led to significant increases (p<0.05) in body weight, hot carcass weight, cold carcass weight, and dressing percentage. CSH supplementation decreased shear force values and cooking loss (p<0.05). Meat lightness was significantly greater in the 1.0% CSH-supplemented group (p<0.05). With increasing CSH levels, the proportion of high-value cuts, such as rib set and T-bone, increased (p<0.05), while the proportion of lower-value cuts, including macreuse, shank, and tendons, decreased (p<0.05). Non-carcass weights, including the feet, head, digestive tract, and scraps, were significantly higher in the 1.0% CSH-supplemented diet (p<0.05). The meat's chemical composition did not differ significantly among the treatment groups (p>0.05). Muscle fiber diameter was significantly larger in the 1.0% CSH-supplemented group (p<0.05). Monounsaturated fatty acids increased (p<0.05) with higher CSH levels, whereas polyunsaturated and saturated fatty acids exhibited a significant decrease (p<0.05).</p><p><strong>Conclusion: </strong>Dietary supplementation with CSH enhances growth rate, carcass traits, and meat quality in steers. An inclusion level of 1.0% CSH in concentrate is the optimum feeding dosage, demonstrating its potential as an effective feed additive for enhancing beef production efficiency.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2136-2148"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phuphan chicken breeds: classification as varieties or distinct breeds with three derivative groups using microsatellite genotyping.","authors":"Ekerette Ekerette, Nivit Tanglertpaibul, Trifan Budi, Wisut Auekingpetch, Chien Phuoc Tran Nguyen, Worapong Singchat, Wongsathit Wongloet, Nichakorn Kumnan, Piangjai Chalermwong, Anh Huynh Luu, Thitipong Panthum, Aingorn Chaiyes, Kanithaporn Vangnai, Chotika Yokthongwattana, Chomdao Sinthuvanich, Narongrit Muangmai, Prateep Duengkae, Kornsorn Srikulnath","doi":"10.5713/ab.24.0579","DOIUrl":"10.5713/ab.24.0579","url":null,"abstract":"<p><strong>Objective: </strong>Indigenous and local breeds, such as Phuphan chickens, are vital due to their adaptability and nutritional value. However, the precise origin, historical records, and genetic diversity of Phuphan chickens remain unclear. This study aimed to evaluate origin and genetic diversity of four Phuphan chicken groups from the Phuphan Royal Development Study Centre.</p><p><strong>Methods: </strong>This study assesses four groups of Phuphan chicken: Phuphan black 1 (SK-B1), Phuphan black 2 (KU-BM/F), Phuphan white (KU-WM/F), and Phuphan color (KU-VM/F) using 28 microsatellite markers and comparing them with those of other Thai chicken breeds within \"The Siam Chicken Bioresource Project\" database.</p><p><strong>Results: </strong>The results highlighted significant genetic diversity among these groups (mean expected heterozygosity [He] = 0.623±0.014; Allelic richness [AR] = 4.594±0.124), indicating effective management through the breeding program of the Phuphan Royal Development Study Centre. Population structure analyses revealed distinct gene pools, emphasizing the genetic uniqueness of SK-B1 relative to the other three groups. Bayesian inference validated historical genetic exchanges, primarily among KU-BM/F, KU-WM/F, and KU-VM/F, with limited exchanges involving SK-B1. This suggests that the Phuphan chicken groups share a common lineage, primarily distinguished by variations in plumage color, resulting from residual selection processes. Microsatellite markers pinpointed the loci LEI0234, MCW206, MCW0016, MCW0222, MCW0098, MCW0165, and ADL0278 as potentially subject to directional selection and associated with plumage color variation among the Phuphan chicken groups. Comparative evaluations with other Thai indigenous local chickens and red junglefowl revealed a closer affinity of SK-B1 to existing Thai chicken breeds, suggesting it may represent a variant of these breeds. Alternatively, KU-BM/F, KU-WM/F, and KU-VM/F, which exhibited comparable external characteristics, may constitute a novel breed of Phuphan chicken.</p><p><strong>Conclusion: </strong>The findings may enhance understanding on genetic architecture of Phuphan chicken groups and contribute to Thailand's economic growth while preserving the genetic diversity of the indigenous chickens.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2055-2066"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}