Animal Bioscience最新文献

{"title":"Intracellular domain of CATSPER1 could serve as a cytoplasmic platform for redox processes in mammalian sperm.","authors":"Jingon Kim, Jae Yeon Hwang","doi":"10.5713/ab.24.0631","DOIUrl":"https://doi.org/10.5713/ab.24.0631","url":null,"abstract":"<p><strong>Objective: </strong>Mammalian sperm acquire fertilizing ability in the female reproductive tract and develop hyperactivated motility, which is indispensable for male fertility. Hyperactivated motility is initiated by Ca2+ influx via the sperm-specific ion channel, CatSper. CATSPER1, a CatSper pore subunit, possesses a long N-terminal intracellular domain and its degradation correlates with unsuccessful sperm migration in the female tract. However, the cellular function and molecular significance of the CATSPER1 N-terminal domain are not well understood. Here, we identify the interactome of the CATSPER1 N-terminal domain and propose a function for the intracellular domain in mammalian sperm.</p><p><strong>Methods: </strong>To identify CATSPER1 N-terminus interactome, we produced recombinant CATSPER1-N-terminus in bacterial system. The purified protein was incubated with testicular lysates and eluted together with testicular interacting proteins. The elutes were subjected to proteomic analysis and CATSPER1-N-terminus interactome was profiled. Identified proteins were further analyzed by functional annotation.</p><p><strong>Results: </strong>We purified the partial CATSPER1 N-terminal domain and identified 57 testicular proteins as domain interactomes using mass spectrometry analysis. Functional annotation analysis revealed that 106 gene ontologies were significantly enriched, 16 of which were related to redox processes. We found that antioxidant enzymes, such as PARK7 and PRDX2, 4, and 6, were included in the enriched redox-related gene ontologies.</p><p><strong>Conclusion: </strong>These results suggest that the CATSPER1 N-terminus could function in defending against oxidative stress to support the successful migration of mammalian sperm to fertilizing sites in the female reproductive tract.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N6-methyladenosine (m6A)-circHECA from secondary hair follicle of cashmere goats: identification, regulatory network and expression regulated potentially by methylation of its host gene promoter.","authors":"Jincheng Shen, Taiyu Hui, Man Bai, Yixing Fan, Yubo Zhu, Qi Zhang, Ruqing Xu, Jialiang Zhang, Zeying Wang, Wenxin Zheng, Wenlin Bai","doi":"10.5713/ab.24.0081","DOIUrl":"10.5713/ab.24.0081","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to identify the N6-methyladenosine (m6A)- circHECA molecule in secondary hair follicles (SHFs) of cashmere goats, and generate its potential regulatory network, as well as explore the potential relationship between transcriptional pattern of m6A-circHECA and promoter methylation of its host gene (HECA).</p><p><strong>Methods: </strong>The validation of circHECA m6A sites was performed using methylation immunoprecipitation (Me-RIP) along with reverse transcription-quantitative polymerase chain reaction (RT-qPCR) technique. The nucleus and cytoplasm localizations of m6AcircHECA were performed using SHF stem cells of cashmere goats with RT-qPCR analysis. Based on in-silico analysis, the regulatory networks of m6A-circHECA were generated with related signal pathway enrichment. The methylation level of promoter region of m6A-circHECA host gene (HECA) was assessed by the bisulfite sequencing PCR (BSPPCR) technique.</p><p><strong>Results: </strong>The m6A-circHECA was confirmed to contain four m6A modification sites including m6A-213, m6A-297, m6A-780, and m6A-927, and it was detected mainly in cytoplasm of the SHF stem cells of cashmere goats. The integrated regulatory network analysis showed directly or indirectly complex regulatory relationships between m6A-circHECA of cashmere goats and its potential target molecules: miRNAs, mRNAs, and proteins. The regulatory network and pathway enrichment indicated that m6A-circHECA might play multiple roles in the SHF physiology process of cashmere goats through directly or indirectly interacting or regulating its potential target molecules. A higher methylation level of promoter region of HECA gene in SHFs of cashmere goats might cause the lower expression of m6A-circHECA.</p><p><strong>Conclusion: </strong>The m6A-circHECA might play multiple roles in SHF physiology process of cashmere goats through miRNA mediated pathways along with directly or indirectly interaction with its target proteins. The promoter methylation of m6A-circHECA host gene (HECA) most likely was implicated in its expression inhibition in SHFs of cashmere goats.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2066-2080"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Samae Dam chicken: a variety of the Pradu Hang Dam breed revealed from microsatellite genotyping data.","authors":"Nivit Tanglertpaibul, Trifan Budi, Chien Phuoc Tran Nguyen, Worapong Singchat, Wongsathit Wongloet, Nichakorn Kumnan, Piangjai Chalermwong, Anh Huynh Luu, Kantika Noito, Thitipong Panthum, Pish Wattanadilokchatkun, Anuphong Payopat, Natthamon Klinpetch, Aingorn Chaiyes, Kanithaporn Vangnai, Chotika Yokthongwattana, Chomdao Sinthuvanich, Syed Farhan Ahmad, Narongrit Muangmai, Kyudong Han, Mitsuo Nunome, Akihiko Koga, Prateep Duengkae, Sompon Waipanya, Yoichi Matsuda, Kornsorn Srikulnath","doi":"10.5713/ab.24.0161","DOIUrl":"10.5713/ab.24.0161","url":null,"abstract":"<p><strong>Objective: </strong>The remarkable adaptability to the environment, high growth rate, meat with good taste and aroma, and ornamental appearance of the Pradu Hang Dam (PDH) and Samae Dam (SD) chickens make them valuable for improvement of poultry production to enhance food security. However, despite their close phenotypic similarity, distinct classification of PDH and SD chickens remains controversial. Thus, this study aimed to clarify genetic origins and variation between PDH and SD chickens, genetic diversity and structures of PDH and SD chickens.</p><p><strong>Methods: </strong>This study analyzed 5 populations of PDH and 2 populations of SD chickens using 28 microsatellite markers and compared with those of other indigenous and local chicken breeds using Thailand's \"The Siam Chicken Bioresource Project\" database.</p><p><strong>Results: </strong>Considerably high genetic variability was observed within PDH (370 total alleles; 4.086±0.312 alleles/locus) and SD chickens (179 total alleles; 3.607±0.349 alleles/locus). A partial overlap of gene pools was observed between SD chickens from the Department of Livestock, Uthai Thani (SD1) and PDH chickens, suggesting a potentially close relationship between the two chicken breeds. A gene pool that partially overlapped with that of the red junglefowl was observed in the SD chicken population from the Sanhawat Farm Uthai Thani population (SD2). Distinct subclusters were observed within SD chickens, indicating the possibility that genetic differentiation occurred early in the process of establishment of SD chickens.</p><p><strong>Conclusion: </strong>These findings could offer valuable insights into genetic verification of Thai local chicken breeds and their sustainable conservation and utilization.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2033-2043"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association study comparison analysis based on Hanwoo full-sib family.","authors":"Ji-Yeong Kim, Eun-Ho Kim, Ho-Chan Kang, Cheol-Hyun Myung, Il-Keun Kong, Hyun-Tae Lim","doi":"10.5713/ab.24.0303","DOIUrl":"10.5713/ab.24.0303","url":null,"abstract":"<p><strong>Objective: </strong>The improvement of carcass traits is essential for the Hanwoo industry because of the Hanwoo grade determination system, and genome-wide association study (GWAS) analysis is an instrumental tool for identifying the genetic factors that impact these traits. While GWAS analysis utilizing family data offers advantages in minimizing genetic bias, research on family-based GWAS in Hanwoo is currently lacking.</p><p><strong>Methods: </strong>This study classified Group A using both parental and offspring genetic information, and Group B based solely on offspring genetic information, to compare GWAS analysis results of Hanwoo carcass traits.</p><p><strong>Results: </strong>A total of 16 significant single nucleotide polymorphism (SNP) markers were identified in Group A, comprising 7 for carcass weight (CWT), 3 for back fat thickness (BFT), and 6 for marbling score (MS). In Group B, 7 significant SNP markers were identified, including 3 for CWT, 1 for eye muscle area, 1 for BFT, and 2 for MS. Functional annotation analysis revealed only one common function related to carcass traits between the groups, while protein-protein interaction analysis indicated more gene interactions in Group A. The reliability of estimated values for common SNP markers identified between the groups was higher in Group A.</p><p><strong>Conclusion: </strong>GWAS analysis utilizing parental genetic information holds greater potential for application, owing to its higher reliability of estimated values and the ability to explore numerous candidate genes.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2054-2065"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Mulberry leaf 1-deoxynijirimycin supplementation shortens villus height and improves intestinal barrier in fattening rabbits.","authors":"Shaocong Li, Tao Li, Zijie Jiang, Wenyu Hou, Qirui Hou, Boris Ramos Serrano, Adileidys Ruiz Barcenas, Yuhua Wang, Weiguo Zhao","doi":"10.5713/ab.24.0109","DOIUrl":"10.5713/ab.24.0109","url":null,"abstract":"<p><strong>Objective: </strong>The current study investigated the effects of mulberry 1-deoxynijirimycin (DNJ) on the digestion ability, intestinal morphology, and intestinal barrier of rabbits.</p><p><strong>Methods: </strong>A total of 36 New Zealand White rabbits (male) about 45 days old (mean body weight of 1.05±0.04 kg) were reared and commercial diets were employed, and afterwards divided into three groups (n = 12) with different levels of DNJ extract additive in feed: T0 (0 g/kg), T1 (0.35 g/kg), T2 (0.7 g/kg) for 28 d.</p><p><strong>Results: </strong>The results demonstrated that T2 decreased the average daily gain (p<0.05). T1 and T2 decreased villus height and inflammatory factor levels as compared with T0 (p<0.05). DNJ significantly decreased the content of valeric acid (p<0.05). The content of acetic acid, propionic acid, iso butyric acid, iso valeric acid in T1 were higher than those in T0 and T2 (p<0.05). The content of butyric acid in T2 was lower than it in T0 and T1 (p<0.05). The content of caproic acid was firstly improved then reduced as the DNJ concentration improved (p<0.05). T2 significantly increased the abundance of dgA-11_gut_group and Christensenellaceae_R-7_group while decreased Bacteroide and Ralstonia as compared with T0 (p<0.05). Compared with T0, T1, and T2 significantly improved the gene expression of JAM2, JAM3, mucin4, mucin6 (p<0.05), T1 significantly decreased the expression of occluding while T2 significantly increased (p<0.05), T2 significantly increased the expression of claudin1 and claudin2 (p<0.05).</p><p><strong>Conclusion: </strong>DNJ at high level changed microbiome compositions, inhibited inflammation, and improved intestinal barrier while it decreased the growth performance and shorted villus height in rabbit jejunum by regulating short chain fatty acid compositions in rabbits.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2101-2112"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of acute and chronic heat stress on the rumen microbiome in dairy goats.","authors":"Min Li, Lian-Bin Xu, Chen Zhang, Pei-Hua Zhang, Sha Tao, Hong-Yun Liu","doi":"10.5713/ab.24.0120","DOIUrl":"10.5713/ab.24.0120","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to reveal the influence of acute and chronic heat stress (HS) on the abundance and function of rumen microbiome and host metabolism.</p><p><strong>Methods: </strong>Forty mid-lactation goats were randomly divided into two artificial environments: control group and heat-stressed group. This study was recorded from two periods, 1 day and 28 days. The first day was defined as control 1 (CT1) and HS 1 (acute HS), and the last day was defined as CT28 and HS28 (chronic HS). On the first and last day, 6 dairy goats in each group were randomly selected to collect rumen liquid after the morning feeding through oral stomach tubes. The barn temperature and humidity were recorded every day.</p><p><strong>Results: </strong>Disruption of the rumen microbiome was observed under chronic HS, represented by an increase in the abundance of Prevotella and Bacteroidales (p<0.05), and upregulation of carbohydrate transport and metabolism functions (p<0.05). Additionally, the abundance of Succinimonas and Ruminobacter in chronic HS is lower than in acute HS (p<0.05), and the functions of intracellular trafficking, secretion and vesicular transport, and the cytoskeleton were downregulated (p<0.05).</p><p><strong>Conclusion: </strong>The HS affected the interaction between the microbiota and host, thereby regulated milk production in dairy goats. These findings increased understanding of the crosstalk between hosts and bacteria.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2081-2090"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of single nucleotide polymorphisms in differentially expressed genes and proteins reveals the genetic basis of skeletal muscle growth differences between Tibetan and Large White pigs.","authors":"Heli Xiong, Yan Zhang, Zhiyong Zhao","doi":"10.5713/ab.24.0135","DOIUrl":"10.5713/ab.24.0135","url":null,"abstract":"<p><strong>Objective: </strong>Skeletal muscle growth is an important economic trait for meat production, with notable differences between Tibetan pigs (TIBPs, a slow-growing breed) and Large White pigs (LWPs, a fast-growing breed). However, the genetic underpinnings of this disparity remain unclear.</p><p><strong>Methods: </strong>In the current study, we integrated differentially expressed genes (DEGs) and proteins (DEPs) from 60-day-old embryonic muscle tissue, along with whole-genome single nucleotide polymorphisms (SNPs) displaying absolute allele frequency differences (ΔAF) of 0.5 or more between the TIBP and LWP breeds, to unravel the genetic factors influencing skeletal muscle growth.</p><p><strong>Results: </strong>Our analysis revealed 3,499 DEGs and 628 DEPs with SNPs having a ΔAF equal to or greater than 0.5. Further functional analysis identified 145 DEGs and 23 DEPs involved in biological processes related to skeletal muscle development, and 22 DEGs and 3 DEPs implicated in the mechanistic target of rapamycin kinase signaling pathway, which is known for positively regulating protein synthesis. Among these genes, several DEGs and DEPs, enriched with TIPB-specific SNPs in regulatory or/and coding regions, showed marked ΔAF between the TIBP and LWP breeds, including MYF5, MYOF, ASB2, PDE9A, SDC1, PDGFRA, MYOM2, ACVR1, ZIC3, COL11A1, TGFBR1, EDNRA, TGFB2, PDE4D, PGAM2, GRK2, SCN4B, CACNA1S, MYL4, IGF1, and FOXO1. Additionally, genes such as CAPN3, MYOM2, and PGAM2, identified as both DEPs and DEGs related to skeletal muscle development, contained multiple TIBP-specific and LWP-predominant SNPs in regulatory and/or coding regions, underscoring significant ΔAF differences between the two breeds.</p><p><strong>Conclusion: </strong>This comprehensive investigation of SNPs in DEGs and DEPs identified a significant number of SNPs and genes related to skeletal muscle development during the prenatal stage. These findings not only shed light on potential causal genes for muscle divergence between the TIBP and LWP breeds but also offer valuable insights for pig breeding strategies aimed at enhancing meat production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2021-2032"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The T-cell receptor β chain CDR3 insights of bovine liver immune repertoire under heat stress.","authors":"Linhu Hui, Fengli Wu, Yuanyuan Xu, Guangjun Yang, Qiaorong Luo, Yangyang Li, Long Ma, Xinsheng Yao, Jun Li","doi":"10.5713/ab.24.0152","DOIUrl":"10.5713/ab.24.0152","url":null,"abstract":"<p><strong>Objective: </strong>The liver plays a dual role in regulating temperature and immune responses. Examining the influence of heat stress (HS) on liver T cells contributes significantly to understanding the intricate interplay between the immune system and hepatic tissues under thermal stress. This study focused on investigating the characteristics of the T-cell receptor (TCR) β chain CDR3 repertoire in bovine liver samples under both HS and pairfed (PF) environmental conditions.</p><p><strong>Methods: </strong>Sequencing data from six samples sourced from the GEO database underwent annotation. Utilizing immunarch and VDJtool software, the study conducted comprehensive analyses encompassing basic evaluation, clonality assessment, immune repertoire comparison, diversity estimation, gene usage profiling, VJ gene segment pairing scrutiny, clonal tracking, and Kmers analysis.</p><p><strong>Results: </strong>All four TCR chains, namely α, β, γ, and δ, were detected, with the α chains exhibiting the highest detection frequency, followed closely by the β chains. The prevalence of αβ TCRs in bovine liver samples underscored their crucial role in governing hepatic tissue's physiological functions. The TCR β CDR3 repertoire showcased substantial inter-individual variability, featuring diverse clonotypes exhibiting distinct amino acid lengths. Intriguingly, HS cattle displayed heightened diversity and clonality, suggesting potential peripheral T cell migration into the liver under environmental conditions. Notably, differential VJ gene pairings were observed in HS cattle compared to the PF, despite individual variations in V and J gene utilization. Additionally, while most high-frequency amino acid 5-mers remained consistent between the HS and PF, GELHF, and YDYHF were notably prevalent in the HS group. Across all samples, a prevalent trend of high-frequency 5mers skewed towards polar and hydrophobic amino acids was evident.</p><p><strong>Conclusion: </strong>This study elucidates the characteristics of liver TCR β chain CDR3 repertoire under HS conditions, enhancing our understanding of HS implications.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2178-2188"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of benzoic acid in pig diets on nitrogen utilization, urinary pH, slurry pH, and odorous compounds.","authors":"Seung Bin Yoo, Yoon Soo Song, Siyoung Seo, Sung Woo Kim, Beob Gyun Kim","doi":"10.5713/ab.24.0216","DOIUrl":"10.5713/ab.24.0216","url":null,"abstract":"<p><strong>Objective: </strong>The objective was to investigate the effects of dietary benzoic acid (BA) on nitrogen (N) balance, urinary pH, slurry pH, and odorous compounds in feces and slurry of pigs.</p><p><strong>Methods: </strong>Twelve barrows with an initial body weight of 56.2±2.6 kg were individually housed in metabolism crates. The animals were allocated to a replicated 6×4 incomplete Latin square design with 12 animals, 6 experimental diets, and 4 periods, resulting in 8 observations per treatment. The basal diet consisted mainly of corn, soybean meal, and rapeseed meal. Benzoic acid was supplemented to the basal diet at 0.5%, 1.0%, 1.5%, 2.0%, or 2.5% at the expense of corn starch. Each period consisted of a 4-day adaptation period and a 24-h collection period for slurry, followed by a 4-day collection period for feces and urine. On day 5, feces and urine were collected for 24 h and mixed to obtain slurry samples.</p><p><strong>Results: </strong>The daily digested N linearly increased (p<0.05) with increasing BA supplementation. Supplemental BA lowered urinary pH (p<0.001) and slurry pH (p<0.05) in a linear and quadratic manner. In the fecal samples, the concentrations of acetate, propionate, butyrate, and skatole linearly decreased (p<0.05) with supplemental BA. In the slurry samples, the concentrations of butyrate, isovalerate, and skatole linearly decreased (p<0.05) by supplemental BA.</p><p><strong>Conclusion: </strong>In conclusion, supplemental benzoic acid has the potential to improve nitrogen digestion in a dose-dependent manner for pigs. Additionally, dietary benzoic acid lowers urinary pH, slurry pH, and concentrations of odorous compounds in pig feces and slurry.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2137-2144"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forage quality and fermentation dynamics of silages of Italian ryegrass (Lolium multiflorum Lam.) wilted for varying periods.","authors":"Yan Fen Li, Li Li Wang, Young Sang Yu, Xaysana Panyavong, Li Zhuang Wu, Jong Geun Kim","doi":"10.5713/ab.24.0251","DOIUrl":"10.5713/ab.24.0251","url":null,"abstract":"<p><strong>Objective: </strong>This trial was conducted to explore the impact of different wilting time of Italian ryegrass (IRG) in the field on the nutritional quality and fermentation characteristics of its silage.</p><p><strong>Methods: </strong>The harvested IRG was directly wilted in the field for 0 day (W0), 1 day (W1), 2 days (W2), and 3 days (W3), respectively, and tedded every 6 hours. And the preserved IRG was sampled at 1, 2, 3, 5, 10, 20, 30, and 45 days after ensiling and three replicates per treatment.</p><p><strong>Results: </strong>With the extension of wilting, the dry matter (DM) content and pH value of wilted IRG gradually increased (p<0.05). There was a downward trend in; neutral detergent fiber (NDF), acid detergent fiber (ADF), and hemicellulose with the increase of wilting time, but only W2 and W3 were significantly different from W0 (p<0.05). Crude protein (CP), in vitro DM digestibility), total digestible nutrients (TDN), and relative feed value decreased significantly with the increase of wilting time (p<0.05), except for W1. After 45 days of ensiling, W1 had the highest CP, TDN, and the lowest ADF and NDF. During ensiling, the increase of acetic acid and the decrease of WSC in W0 and W1 were similar, but the accumulation rate of lactic acid in W0 was faster than that in W1, resulting in the lowest pH value in W0. After 5 days of ensiling, the ratio of lactic acid to acetic acid in W1 stabilized at around 3:1, while W0 kept changing.</p><p><strong>Conclusion: </strong>Italian ryegrass that wilted in the field for 1 day effectively improved the dynamic changes in CP, TDN, ADF, and NDF and fermentation quality of silage. Therefore, in practice, W1 was more recommended in production of IRG silage.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2091-2100"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}