Animal Bioscience最新文献

{"title":"Effects of bacteriophage cocktail supplementation in gestation diet on reproductive performance, blood profile, milk composition, and fecal microflora of sows.","authors":"Jinsu Hong, Geon Il Lee, Jae-Cheol Jang, Yoo Yong Kim","doi":"10.5713/ab.24.0796","DOIUrl":"10.5713/ab.24.0796","url":null,"abstract":"<p><strong>Objective: </strong>The present study aimed to evaluate the effects of bacteriophage cocktail supplementation in diet of gestation sows on both the sows and their progeny.</p><p><strong>Methods: </strong>A total of 57 F1 multiparous sows (Yorkshire×Landrace) were allotted to one of three treatment groups in a completely randomized design. The sows were fed cornsoybean meal-based diet supplemented with 0%, 0.05%, or 0.10% of a bacteriophage cocktail during the gestation, followed by a common diet during lactation. Body weight and backfat thickness were measured during the trial along with blood collection for immunoglobulin analysis. Fecal samples were collected on 4, 6, 8, and 10 weeks of gestation period to examine fecal microflora. Litter performance and milk composition were investigated at 24 hrs postpartum and on d 21 of lactation.</p><p><strong>Results: </strong>Increasing bacteriophage cocktail supplementation levels in gestation diets resulted in linear increas (p<0.05) in feed intake of lactating sows and in the weight of live litters, while showing quadratic increas (p<0.05) in litter weight gain from d 0 to 21 of lactation. Dietary treatment had no impact on the serum concentrations of IgG and IgM in the sows. Increasing dietary bacteriophage cocktail levels in gestation diets resulted in a linear increas (p<0.05) in the fat content of colostrum. The elevation of dietary bacteriophage cocktail in microflora of sows resluted in a significant increase (p<0.05) in Lactobacillus count for d 63 and 105 of the gestation period, while concurrently decreasing Escherichia coli (E. coli; p<0.05) and Salmonella (p<0.10) counts during the late gestation period.</p><p><strong>Conclusion: </strong>The supplementation of a 0.05% bacteriophage cocktail in the diet of gestating sows could improve total litter weight, alive litter weight at birth, and litter weight gain during the lactation. This effect is attributed to positive changes in fecal microflora with an increase of Lactobacillus and a decrease of E. coli and Salmonella.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1708-1718"},"PeriodicalIF":2.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular regulatory mechanisms of dietary supplementation with Allium mongolicum Regel powder to improve muscle development and meat quality in Angus calves.","authors":"Wangjing Liu, Chenxu Sun, Huixia Gao, Jianjian He, Aihuan Yu, Yaodi Xie, Haibo Yao, Jiang Hu, Zhaomin Lei","doi":"10.5713/ab.24.0809","DOIUrl":"10.5713/ab.24.0809","url":null,"abstract":"<p><strong>Objective: </strong>Supplementing animal feed with Allium mongolicum Regel powder (AMRP) additives can promote muscle production and improve meat quality. Here, we explored the effects of dietary AMRP supplementation on the performance, meat quality, and muscle transcriptome profile of Angus calves.</p><p><strong>Methods: </strong>Twelve healthy female black Angus calves (average body weight = 280.4±15.74 kg, average age = 14±0.6 months) of the same genetic background were randomly assigned to two feed groups: control (CON; basal diet without any supplementation) and AMRP (basal diet supplemented with 20 g of AMRP per calf per day).</p><p><strong>Results: </strong>In general, compared with the control group, dietary AMRP supplementation significantly increased the longissimus thoracis muscle area (p = 0.027) and pH24h (p = 0.027) but significantly reduced Warner-Bratzler shear force (p = 0.009) and cooking loss (p<0.001). Moreover, 1,284 differentially expressed genes (DEGs) were identified in AMRP-supplemented Angus calves. Pathway analysis revealed that the DEGs were involved in multiple pathways related to muscle development and fat deposition, such as the focal adhesion and MAPK pathways.</p><p><strong>Conclusion: </strong>Dietary supplementation of AMRP improved muscle growth and development in Angus beef cattle. It also significantly modulated meat quality, possibly altering signaling pathways by influencing key gene expression. Our results provide novel insights into the development of the meat industry and indicate the mechanism through which AMRP regulates muscle development and improves meat quality at the molecular level.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1798-1816"},"PeriodicalIF":2.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyrroloquinoline quinone promotes porcine oocyte in vitro maturation and subsequent embryo development by enhancing lipid metabolism and improving mitochondrial function.","authors":"Zehua Zhang, Zhigang Gao, Zhenwei Jia","doi":"10.5713/ab.24.0847","DOIUrl":"10.5713/ab.24.0847","url":null,"abstract":"<p><strong>Objective: </strong>The present study evaluated the beneficial effects of pyrroloquinoline quinone (PQQ) on in vitro maturation (IVM) of porcine oocyte and subsequent early embryo development.</p><p><strong>Methods: </strong>Porcine cumulus oocyte complexes were cultured in IVM medium with supplementation of 0, 200, 400, 800 or 1600 nM PQQ for 42 h. We first examined cumulus expansion index (CEI) and the rate of oocyte nuclear maturation. Then, we assessed oocyte mitochondrial function, oxidative stress levels, lipid metabolism and subsequent embryonic development.</p><p><strong>Results: </strong>PQQ (800 nM) supplementation significantly increased CEI and the nuclear maturation rate of oocytes following IVM. Additionally, oocysts supplemented with 800 nM of PQQ showed significantly increased mitochondrial content, mitochondrial membrane potential, activity, and mRNA expression levels of genes associated with mitochondrial biogenesis (PGC-1a, NRF1, NRF2 and TFAM). PQQ significantly reduced the levels of reactive oxygen species, lipid drop-lets, and fatty acids content, while enhancing the mRNA expression levels of genes related to antioxidant activity (SOD1, SOD2, GPX and CAT), lipolysis (ATGL and HSL) and β-oxidation (CPT1B and CPT2) in porcine oocytes. PQQ (800 nM) supplementation significantly increased cleavage rate, blastocyst formation rate, and total blastocyst cell numbers following partheno-genetic activation.</p><p><strong>Conclusion: </strong>PQQ supplementation during IVM positively influences porcine oocyte maturation and subse-quent embryonic development by enhancing mitochondrial function and lipid metabolism and alleviating oxidative stress.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1644-1656"},"PeriodicalIF":2.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229910/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serotonin promotes calcium accumulation and inhibits lipid accumulation in cultured goat mammary epithelial cells through HTR2A.","authors":"ZhiFei Zhang, BinHan Li, YingFei Wang, Tumaresi Abuduwufuer, Kang Hu, HuiLing Zheng","doi":"10.5713/ab.24.0792","DOIUrl":"10.5713/ab.24.0792","url":null,"abstract":"<p><strong>Objective: </strong>Fatty acids and calcium are both important nutrients in goat milk. Investigating the upstream molecular regulatory mechanisms that control the synthesis of milk fat and milk calcium in the mammary gland can help improve the quality of milk at its source. The objective of this study was to investigate the effects and regulatory pathways of serotonin (5-hydroxytryptamine, 5-HT) and its receptors on lipid synthesis and calcium ion levels in goat mammary epithelial cells (GMECs).</p><p><strong>Methods: </strong>GMECs isolated from live goats were treated with serotonin, overexpression of Serotonin receptor 2A (HTR2A), Sarpogrelate ([SAR] the specific antagonist of HTR2A), or a combination of these agents. The expression of genes related to de novo lipid synthesis in GMECs were detected using the Quantitative Real-Time polymerase chain reaction, the content of lipid droplets was detected using the BODIPY assay, and the calcium content was detected using the calcium chelating probe Fluo-3AM assay.</p><p><strong>Results: </strong>5-HT dose-dependently promotes the activity of GEMCs, significantly inhibits the mRNA expression of key genes involved in de novo lipid synthesis such as ACC, FASN, SREBP1, SCD1 and ELOVL6 at a concentration of 100 μM, reduces triglyceride and total cholesterol content, suppresses lipid droplet accumulation in cells, and simultaneously promotes calcium accumulation in cells. Furthermore, overexpression of HTR2A in GMECs also induces an increase in cellular calcium levels and inhibits lipid synthesis and accumulation in cells. However, treatment of cells with SAR, the specific antagonist of HTR2A, significantly increases the levels of triglycerides, total cholesterol, and lipid droplet accumulation in cells.</p><p><strong>Conclusion: </strong>5-HT inhibits lipid synthesis in GMECs while promoting an increase in cellular calcium levels, and this effect is mediated by the HTR2A receptor. Furthermorey, antagonists targeting HTR2A can reverse the inhibition of lipid synthesis and accumulation in cells.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1633-1643"},"PeriodicalIF":2.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effect of corn straw and mulberry leaves on in vitro fermentation, growth performance and antioxidant capacity of sheep.","authors":"Qirui Hou, Wenfeng Zhang, Shuli Wang, Wenyu Hou, Weiguo Zhao, Hui Xie, Peng Zhao, Yongen Zhang","doi":"10.5713/ab.24.0763","DOIUrl":"10.5713/ab.24.0763","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the synergistic effects of corn straw and mulberry leaves on the growth performance and antioxidant capacity of sheep.</p><p><strong>Methods: </strong>Corn straw and mulberry leaves were mixed in six different ratios as culture substrates: 100:0 (T0), 80:20 (T20), 60:40 (T40), 40: 60 (T60), 20:80 (T80) and 0:100 (T100). Rumen fluid from small-tailed Han sheep was collected, and artificial saliva was prepared. Gas production (GP) parameters, volatile fatty acids (VFAs), ammonia nitrogen (NH3-N), microbial crude protein (MCP) and additional indicators of in vitro rumen fluid fermentations were measured over 72 h. Combinations with a positive multiple-factors associative effect index were chosen for a 65 d in vivo feeding experiment, during which the growth performance, carcass traits, meat quality, and liver antioxidant function were evaluated.</p><p><strong>Results: </strong>Mulberry leaves exhibited a positive correlation with in vitro theoretical maximum GP, cumulative GP, MCP, and organic matter digestibility. At 72 h, total VFA and acetic acid were significantly higher in the T60, T80, and T100 groups (p<0.05). Additionally, at 6 h, 12 h, and 72 h, the NH3-N of the T60, T80, and T100 groups were significantly higher (p<0.05). The T20, T60, and T80 combinations were selected for sheep feeding, and T60 got the highest increase in average daily feed intake (p<0.05). Moreover, the thickness of the rib and back muscle were notably increased in the T60 and T80 groups (p<0.05). Water-holding capacity of the longissimus dorsi muscle was higher and drip loss was lower in the combination-fed groups, particularlly in the T20 and T60 groups (p<0.05). Blood urea nitrogen levels decreased with increasing mulberry leaf content (p<0.05). Furthermore, the T60 and T80 groups demonstrated higher α,α-diphenyl-β-picrylhydrazyl and 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) free radical scavenging ability (p<0.05). Catalase and glutathione peroxidase activities in the sheep liver were also notably enhanced (p<0.05).</p><p><strong>Conclusion: </strong>The combination of corn straw and mulberry leaves had a positive synergistic effect on improving feed intake, muscle thickness, muscle water retention, and the liver antioxidant capacity in sheep. The ratios of 40:60 corn straw to mulberry leaves showed the most beneficial effects. This combination provides a practical method for utilizing agricultural waste and optimizing sheep diets.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1657-1671"},"PeriodicalIF":2.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative effects of replacing soybean meal in feeds with processed soybean meal on intestinal health and growth of nursery pigs when fed pharmacological level of zinc.","authors":"Lan Zheng, Jung Yeol Sung, Sung Woo Kim","doi":"10.5713/ab.24.0767","DOIUrl":"10.5713/ab.24.0767","url":null,"abstract":"<p><strong>Objective: </strong>The objective was to evaluate the effects of partially replacing soybean meal (SBM) in nursery pig diets with enzyme-treated soybean meal (ESBM), fermented soybean meal (FSBM), or fermented soybean meal containing probiotic microorganism (PFSBM) on jejunal mucosa-associated microbiota, immune responses, intestinal morphology, and growth performance of nursery pigs.</p><p><strong>Methods: </strong>Forty-eight weaned pigs (initial body weight = 7.8 ± 0.7 kg) were randomly allocated to four dietary treatments in a randomized complete block design and fed for 25 d in three phases (5, 10, and 12 d, respectively). Dietary treatments were corn-SBM-based diet (SBM diet) and the SBM diet in which 70 g/kg of SBM was replaced with ESBM (ESBM diet), FSBM (FSBM diet), or PFSBM (PFSBM diet). Zinc oxide was supplemented at 2.5 g/kg (2,000 mg/kg of zinc) in the experimental diets for phases 1 and 2. Pigs were housed individually in pens (1.50 m×0.74 m) equipped with a feeder and a nipple drinker. Serum was collected on d 24 and pigs were euthanized on d 27 to collect tissues and mucosa in the jejunum.</p><p><strong>Results: </strong>The PFSBM diet increased (p<0.05) the relative abundance of Lactobacillus, whereas it tended to decrease (p = 0.072) the abundance of Pseudomonas compared with the SBM diet. The ESBM diet increased (p<0.05) gain to feed ratio from d 5 to 15 compared with the SBM diet. However, partially replacing SBM at 70 g/kg with ESBM, FSBM, or PFSBM did not affect immune responses in serum and jejunal mucosa, intestinal morphology in the jejunum, and overall growth performance of nursery pigs.</p><p><strong>Conclusion: </strong>Partially replacing SBM with various processed SBM did not affect immune responses, intestinal morphology, and overall growth performance when diets were supplemented with zinc at pharmacological level in early phases.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1719-1732"},"PeriodicalIF":2.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analysis of muscle transcriptome data identifies key genes influencing intramuscular fat content in pigs.","authors":"Yan Wang, Jiahao Wang, Yanhui Zhao, Xihui Sheng, Xiaolong Qi, Lei Zhou, Jianfeng Liu, Chuduan Wang, Jianliang Wu, Yongchun Cao, Kai Xing","doi":"10.5713/ab.24.0905","DOIUrl":"10.5713/ab.24.0905","url":null,"abstract":"<p><strong>Objective: </strong>The content of intramuscular fat (IMF) is closely linked to meat quality, and the mechanism of IMF deposition is complex. Despite numerous transcriptomic studies on IMF, variations in sample sizes and data analysis methods have produced inconsistent gene expression patterns and results. To identify the pivotal genes influencing pig IMF content, we performed a meta-analysis on 10 pig muscle transcriptome datasets with a total of eighty samples, forty with high and forty with low IMF samples.</p><p><strong>Methods: </strong>DESeq2 has been used to analyze the high and low IMF groups for 10 datasets each, resulting in the differentially expressed genes (DEGs) for each dataset. To identify key genes affecting IMF content, we performed a meta-analysis of the differential expression results from the 10 datasets using MetaVolcanoR. Subsequently, we conducted protein-protein interaction network analysis, Gene Ontology and Kyoto encyclopedia of genes and genomes functional enrichment analysis, and quantitative trait locus (QTL) analysis on the DEGs.</p><p><strong>Results: </strong>The meta-analysis identified 129 DEGs, comprising 71 upregulated and 58 downregulated DEGs in the high IMF group. The DEGs exhibited enrichment in processes associated with adipocyte differentiation and fat anabolism. QTL analysis demonstrated that five DEGs, including FASN and SCD, corresponded to six QTLs associated with IMF.</p><p><strong>Conclusion: </strong>The findings suggest that meta-analysis effectively integrates data from multiple datasets, resulting in more reliable outcomes. This approach enabled the identification of the core gene cluster comprising FASN, SCD, and PLIN1, LEP, and G0S2, which influence IMF content in pigs.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1622-1632"},"PeriodicalIF":2.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of milk fortification with an advanced chelated trace minerals supplement on growth, health, and nutrient digestibility in dairy calves.","authors":"Hossein Rajaei-Sharifabadi, Hadi Rahmani, Zahra Shokri, Saideh Fakharzadeh, Somayeh Kalanaky, Mohammad Hassan Nazaran, Ahmad Reza Seradj","doi":"10.5713/ab.24.0701","DOIUrl":"10.5713/ab.24.0701","url":null,"abstract":"<p><strong>Objective: </strong>Recent advancements in chelate compound technology offer improved bioavailability of trace minerals, potentially enhancing growth performance and reducing disease incidence. Milk fortification is an efficient method to supplement young calves before transition to solid feed. This study evaluated the response of dairy calves to milk fortification with an advanced chelated trace minerals supplement containing Zn, Mn, Cu, Fe, Co, Cr, and Se, each selected for their specific roles in calf development, synthesized using chelate compoundtechnology.</p><p><strong>Methods: </strong>Forty-two Holstein dairy calves (21 males and 21 females) were randomly assigned to three experimental groups: control (CON, unfortified milk), chelated minerals (BON, milk fortified with chelated trace minerals), and inorganic minerals (IOR, milk fortified with inorganic trace minerals). Calves received their respective milk treatments daily from d 3 until weaning at 70 d of age. Growth performance and body measurements were assessed throughout the experiment, while rectal temperature, fecal consistency, and frequency of medical treatment were recorded until d 21. Blood metabolites and nutrient digestibility were also determined.</p><p><strong>Results: </strong>BON supplementation resulted in 9% body weight increase (p = 0.027) and improved some body measurements (p<0.05). The calves in BON group showed a lower fecal consistency index (p<0.05) and required fewer medical treatments compared to CON and IOR (p = 0.103). Blood analysis revealed 27% reduction in globulin levels (p = 0.022), 10% increase in albumin levels (p = 0.023) and a 42% elevation in albumin/globulin ratio (p = 0.042) in BON compared to the CON groups. The activities of liver enzymes were higher in IOR than in the BON and CON group.</p><p><strong>Conclusion: </strong>Milk fortification with advanced chelated trace minerals significantly enhanced growth performance and health indicators in dairy calves, while inorganic sources showed no beneficial effects. These findings establish the superiority of chelated mineral supplementation for early-life dairy calf development;nevertheless, appropriate supplementation procedures require additional experiments.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1694-1707"},"PeriodicalIF":2.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of DNA methylation changes following Cfp1 knockout in mouse spermatocytes.","authors":"Chanhyeok Park, Youngsok Choi, Seonho Yoo, Hyeonwoo La, Kwonho Hong","doi":"10.5713/ab.24.0807","DOIUrl":"10.5713/ab.24.0807","url":null,"abstract":"<p><strong>Objective: </strong>Spermatogenesis is a complex biological process that encompasses meiosis in spermatocytes and the dynamic epigenetic alterations that ensure the inheritance of genetic traits. CXXC finger protein 1 (CFP1, Cfp1, CXXC1, Cxxc1) is a critical component of the SET domain-containing 1A histone lysine methyltransferase complex that catalyzes histone H3K4 methylation and has a specific binding domain for unmethylated CpG DNA. However, our current understanding of CFP1's role in the genome-wide regulation of DNA and H3K4 methylation remains limited.</p><p><strong>Methods: </strong>We performed genome-wide methylation analysis using reduced-representation bisulfite sequencing on spermatocytes isolated from Cfp1 knockout and wild-type mice. Promoter methylation changes were integrated with publicly available microarray and ChIP-seq data to identify genes regulated by CFP1.</p><p><strong>Results: </strong>CFP1 depletion led to significant alterations in DNA methylation, particularly in promoter regions of genes associated with meiosis, transcription regulation, and chromatin remodeling. A total of 21 genes were identified as direct targets of CFP1, exhibiting reduced promoter methylation and CFP1 binding.</p><p><strong>Conclusion: </strong>Our study findings contribute to elucidating the regulatory mechanisms of CFP1 in spermatocytes, providing valuable insights into the reproductive process and advancing our understanding of the underlying causes of infertility.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1570-1579"},"PeriodicalIF":2.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of nitrate in combination with lactate on rumen fermentation and methane production in an in vitro batch culture.","authors":"Beomseok Bae, Logan Pope, Kiyeon Park, Chanhee Lee","doi":"10.5713/ab.25.0020","DOIUrl":"10.5713/ab.25.0020","url":null,"abstract":"<p><strong>Objective: </strong>This in vitro study determined the combination effect of nitrate and lactate on nitrate reduction, rumen fermentation and methane production. We hypothesized that the combination decreases methane production and enhances nitrate reduction.</p><p><strong>Methods: </strong>An in vitro batch culture was conducted to determine the 2 main factors of nitrate and lactate in 2×2 factorial arrangement: a basal diet (CON), the basal diet supplemented with nitrate (2.2% in dietary dry matter [DM]; Nit), lactate (2.2%; Lact), or the combination of nitrate and lactate (Nit-Lact). Rumen fluids from lactating Holstein cows were obtained and mixed with McDougall's buffer for the in vitro batch culture. Total gas production, methane production, pH, volatile fatty acid (VFA), NH3-N, NO3-N and NO2-N concentration, and DM digestibility were determined.</p><p><strong>Results: </strong>A trend of interaction between nitrate and lactate was observed for DM digestibility (p = 0.06), which occurred because DM digestibility tended to decrease for Nit compared with CON (42.2 vs. 45.3%) but did not for Nit-Lact. Nitrate decreased (p<0.05) methane (25.6 vs. 31.0 mL at 24 h) and total gas production (296 vs. 313 mL at 24 h). Total VFA production was not affected by treatments. However, nitrate increased the proportion of acetate (p<0.01) and decreased (p<0.01) proportions of propionate, butyrate and valerate at 24 h of incubation. However, lactate increased valerate at 24 h of incubation (p<0.01). The concentration of NO3-N for Nit-Lact was lower than that of Nit (41.2 vs. 51.0 mg/L; p<0.01) at 3 h of incubation.</p><p><strong>Conclusion: </strong>Nitrate decreased methane production. The combination of nitrate and lactate had beneficial effects on DM digestibility and nitrate reduction, compared with nitrate alone although no interaction between nitrate and lactate was shown for VFA production and methane production. Changes in the major VFA by nitrate indicated that nitrate acted as an alternative electron acceptor to decrease methane production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1672-1679"},"PeriodicalIF":2.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}