Animal Bioscience最新文献

{"title":"Challenges and constraints in the sustainability of poultry farming in Japan.","authors":"Haruhiko Ohtsu","doi":"10.5713/ab.24.0675","DOIUrl":"https://doi.org/10.5713/ab.24.0675","url":null,"abstract":"<p><p>Poultry products such as chicken meat and eggs are among the most common and popular animal products in Japan. Recently, many chickens, such as broilers and layers, have been raised and their related product consumption has increased. However, the number of farms decreased, which is one of the major challenges faced by the Japanese poultry industry. Similar to that in other countries, high-pathogenicity avian influenza (HPAI) outbreaks negatively affected the distribution of poultry products. Low feed self-sufficiency in Japan is also a serious problem because the prices of diets and products are affected by the situation in foreign countries. Rice is a domestic ingredient of the poultry diet in Japan, and recently, its utilization has increased; however, concerns remain. Global warming likely affects the poultry industry in Japan negatively. The objective of this review is to illustrate the recent situation of the Japanese poultry industry, including 1) an overview; 2) the situation of influence of HPAI ; 3) situation of ingredients for poultry diet; 4) utilization of rice in poultry diet; 5) heat stress in poultry. Overall, investigation of the effects of heat stress on physiology, such as the biological defense system, and its prevention, should be continued to prevent future decreases in productivity in the Japanese poultry industry.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges and constraints to the sustainability of poultry farming in Republic of Korea.","authors":"Sidong Kim","doi":"10.5713/ab.24.0641","DOIUrl":"https://doi.org/10.5713/ab.24.0641","url":null,"abstract":"<p><p>As of 2022, Republic of Korea accounted for 0.8% of global chicken meat production and 0.9% of global egg production. The country achieved self-sufficiency rates of 83.1% for chicken meat and 99.4% for eggs, demonstrating significant quantitative and qualitative growth to meet domestic demand. Although the industry is trending towards expansion and specialization, it faces several challenges in achieving sustainable poultry production. Key challenges in Korea include highly pathogenic avian influenza (HPAI) and pest issues, climate change and the push for carbon neutrality, reliance on imported breeding stock, insufficient preparedness for expanding cage space per laying hen, post-settlement payment systems for egg sales and an oversupply of chicken meat, and the aging poultry farming population and the closure of farms unable to secure successors. Following strategies are proposed to overcome or mitigate challenges mentioned above: (1) enhancing farm biosecurity and implementing vaccination policies for disease control, (2) modernizing facilities and promoting carbon-neutral practices to adapt to climate change, (3) diversifying breeding stocks across multiple locations and developing domestic strains, (4) implementing policies and supporting farms based on a comprehensive readiness assessment of all farms regarding expanded cage space requirements, (5) improving market transparency for the egg industry and regulating supply and demand in the broiler industry, and (6) offering incentives for farm succession, attracting labor, and promoting coexistence between corporations, rural communities, and small farms. In conclusion, the sustainable development of Korea's poultry industry is not a simple task. It requires a comprehensive approach considering economic efficiency, animal welfare, environmental protection, food security, and the symbiosis with rural communities. This approach necessitates efficient cooperation among all stakeholders, including the government, farmers, integrators, retailers, and research institutions, along with a comprehensive, phased strategy for both short- and long-term goals.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges and constraints to the sustainability of poultry farming in China.","authors":"Fei Zhang, Zhuting Chen, Jiayi Shi, Chenglong Han, Qinyi Zhan, Zhouzheng Ren, Xiaojun Yang","doi":"10.5713/ab.24.0794","DOIUrl":"https://doi.org/10.5713/ab.24.0794","url":null,"abstract":"<p><p>China's poultry industry is characterized by large-scale production and rich breeds, presenting both opportunities and challenges. In 2023, the industry produced 10.79 billion broilers, 28.38 million tons of eggs, and 4.88 billion waterfowl. The foundation of a thriving poultry industry lies in the continuous improvement of breeds. For instance, new lines of Lueyang black-boned chickens have been developed using genomic selection breeding, with a focus on improving production performance and unlocking their high-quality genetic potential. Precision nutrition programs enhance the expression of poultry's genetic potential and improve feed utilization efficiency. The five-dimensional feed evaluation system and the comprehensive National Feed Database provide formulators with accurate nutritional parameters of feed. Additionally, the concept of 'nutrition power' and the gold standard of the 'intestinal five rings' enable researchers to analyze poultry's digestive physiology more effectively. Feeding management plays a crucial role in optimizing genetic potential and the effectiveness of precision nutrition. To further boost production efficiency, smart farming systems have been implemented, incorporating intelligent management of environmental factors, animal parameters, and poultry health tracking. Meanwhile, in order to improve material utilization efficiency across the entire poultry production chain and support the sustainable development of the poultry industry, it is essential to optimize and promote the application of the poultry-crop interaction system. In summary, strengthening fundamental research in poultry, optimizing smart poultry farm platforms, and implementing poultry-crop interacting systems will drive the sustainable development of China's poultry industry.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges and constraints to the sustainability of poultry farming in Thailand.","authors":"Sawitree Wongtangtintharn, Sudpradthana Chakkhambang, Padsakorn Pootthachaya, Anusorn Cherdthong, Metha Wanapat","doi":"10.5713/ab.24.0685","DOIUrl":"https://doi.org/10.5713/ab.24.0685","url":null,"abstract":"<p><p>The poultry farming industry in Thailand plays a crucial role in the nation's economy and food security. However, its long-term sustainability is challenged by disease outbreaks, environmental concerns, rising feed costs, market fluctuations, and shifting consumer preferences. This review examines the current state of poultry farming in Thailand, focusing on broilers, layers, and ducks. Disease control requires strict biosecurity measures and government interventions. Additionally, the industry must address environmental concerns by reducing greenhouse gas emissions and optimizing resource efficiency to align with Thailand's net-zero targets. The rising demand for organic and ethically produced poultry products presents both market opportunities and production challenges, necessitating significant adjustments in farming practices. Regulatory compliance, particularly in export markets, adds another layer of complexity, requiring Thai poultry producers to meet increasingly stringent international standards. The adoption of new technologies, such as precision farming and AI-driven systems, presents both opportunities and challenges, especially for smaller producers who may struggle to keep pace with advancements. Rising feed costs, driven by global supply chain disruptions and dependence on imported raw materials, pose a significant economic burden, emphasizing the need for alternative protein sources such as insect-based feed and agro-industrial by-products. Furthermore, regulatory compliance with international standards and consumer-driven trends toward antibiotic-free and organic poultry products further shape the industry's transformation. To ensure sustainable development, Thailand's poultry sector must integrate innovative technologies, adopt environmentally friendly practices, and strengthen collaboration between the government, industry stakeholders, and researchers. The industry can maintain its global competitiveness by addressing these challenges while contributing to sustainable food production and environmental conservation.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges and Constraints to the Sustainability of Poultry Farming in Indonesia.","authors":"Sumiati Sumiati, Roni Fadilah, Arif Darmawan, Rizky Nadia","doi":"10.5713/ab.24.0678","DOIUrl":"https://doi.org/10.5713/ab.24.0678","url":null,"abstract":"<p><p>Although the demand for poultry products in Indonesia, one of the most significant poultry-producing countries in Southeast Asia, continues to increase, the poultry farming sector continues to face various challenges. This paper provides an overview of the prospects for and challenges faced by the poultry farming sector in Indonesia. Broilers comprise the largest portion of Indonesia's poultry population, with other commodities contributing less than 10% of the total. During the COVID-19 pandemic, the consumption of chicken meat and eggs increased. The declining national economy led people to consume affordable food for their daily meals, favoring chicken and eggs because their retail prices were four times cheaper than that of beef. In terms of imports to Indonesia, compared with other agricultural commodities, poultry and eggs account for only approximately 1.26% and 0.41%, respectively. Meanwhile, some feed ingredients, such as soybean meal and corn, continue to be imported into Indonesia. According to data from the United States Department of Agriculture, Indonesia imported 4.4 million tons of soybean meal to meet the needs of its poultry industry. In recent years, feed mill companies have rapidly been developing in Indonesia, with an estimated average production of 572,000 metric tons. In terms of scale, poultry farming in Indonesia comprises 60% large-scale farming and 40% small- and medium-scale farming. Many small-scale farms have yet to implement strict biosecurity measures, making climate change a significant challenge for poultry farming in Indonesia. After implementing the ban on antibiotic growth promoters, Indonesia's poultry farming sector began to stabilize and look for alternative methods to maintain productivity. The use of technology, improved regulations, and the enhancement of farmers' capacity to manage challenges are key toward sustaining and increasing poultry farming productivity in Indonesia in the future.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"circRNA18_46222157_46248185 inhibits melanogenesis by targeting miR-211/EP300 pathway in goat melanocytes.","authors":"Kai Yuan Ji, Xue Qing Zhang, Yi Wei Zhao, Chun E Liang, Xin Yuan, Yun Hai Zhang","doi":"10.5713/ab.24.0316","DOIUrl":"10.5713/ab.24.0316","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the effects of circRNA18_46222157_46248185 (named circRNA18) on goat melanogenesis, which differs significantly in goat skins isolated from white and brown coat-colored skins.</p><p><strong>Methods: </strong>Expression patterns of circRNA18 in goat skin and melanocytes were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and in situ hybridization. The circRNA18 interference vector was designed and synthesized to transfect melanocytes and detect the effect of circRNA18 interference on melanin production. Bioinformatics software was used to predict the targeted adsorption miRNAs of circRNA18, verified by luciferase assay. A miRNA expression vector was constructed and transfected into melanocytes to detect the effect of miRNA on melanin production, and the targeted regulatory genes were detected by luciferase assay. Target gene interference vector was constructed to detect the influence of target gene interference on melanin production.</p><p><strong>Results: </strong>qRT-PCR results unveiled distinct expression patterns of circRNA18 in diverse tissues of male and female goats, while in situ hybridization assays showed that circRNA18 is expressed in the cytoplasm of melanocytes. Functional analysis demonstrated that the downregulation of circRNA18 in melanocytes leads to a significant increase (p<0.01) in melanin production. Bioinformatics analysis identified a potential miR-211 binding site on circRNA18, and luciferase assay confirmed their interaction. Overexpression of miR-211 in melanocytes significantly augmented (p<0.01) melanin production. There were two potential miR-211 binding sites on adenoviral E1A-binding protein (EP300), and the overexpression of miR-211 in melanocytes significantly decreased (p<0.001) EP300 expression, with luciferase assay confirming their interaction. Downregulation of EP300 expression in melanocytes through siRNA-EP300 transfection results in a substantial increase (p<0.05) in melanin production. qRT-PCR results indicated that overexpression of mimics-circRNA18 in melanocytes markedly suppressed (p<0.0001) miR-211 expression, significantly elevated (p<0.01) EP300 expression, and significantly inhibited (p<0.001) melanin production.</p><p><strong>Conclusion: </strong>circRNA18_46222157_46248185 acted as a negative regulator of melanogenesis in goat melanocytes by targeting the miR-211/EP300 pathway, and guiding animal hair color breeding strategies.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"255-266"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of immune-associated genes with altered expression in the spleen of mice enriched with probiotic Lactobacillus species using RNA-seq profiling.","authors":"Anh Duc Truong, Ha Thi Thanh Tran, Nhu Thi Chu, Lanh Phan, Hoai Thi Phan, Thu Huong Dang, Hoang Vu Dang, La Anh Nguyen","doi":"10.5713/ab.24.0280","DOIUrl":"10.5713/ab.24.0280","url":null,"abstract":"<p><strong>Objective: </strong>Probiotics are living microorganisms that can provide health benefits when consumed. Here, we investigated the effects of probiotics on gene expression in the spleen of mice using RNA-sequencing analysis between negative control and probiotic groups (including 4 Lactobacillus strains: Lactobacillus fermentum, L. casei, L. plantarum, and L. brevis).</p><p><strong>Methods: </strong>Mice exposed with probiotic in 4 weeks by intragastric administration. Then, spleen tissues of the control and probiotics groups were collected on days 14 and 28 for RNA sequencing.</p><p><strong>Results: </strong>In total, 665, 186, and 81 differentially expressed genes (DEGs) were significantly expressed on day 14 vs control, day 28 vs control groups, and probiotics day 28 vs day 14 groups, respectively. On the other hand, 12 toll-like receptor genes underwent additional validation through quantitative real-time polymerase chain reaction (qRT-PCR), affirming the increased alignment between qRT-PCR and RNA-Seq findings. In addition, the Kyoto encyclopedia of genes and genomes and gene ontology analyses revealed that the DEGs were predominantly enriched in defense responses to pathogens, including inflammatory bowel diseases, malaria, leukaemia virus 1, and herpes virus, as well as immune processes related to immune response and signal transduction. This study represents the first investigation into mice's gene expression in the spleen exposed to probiotics using Lactobacillus spp. isolated from a field strain in Vietnam.</p><p><strong>Conclusion: </strong>Our results provide valuable insights into the impacts and functions of probiotics on mammalian development, offering crucial information for the potential therapeutic use of probiotics in defending against pathogens in Vietnam. The findings from this study highlight the potential of probiotics in modulating gene expression in the spleen, which may have implications for immune function and overall health in mice.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"336-349"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary effects of protected fat, soybean meal, and heat-treated soybean meal on performance, physiological parameters, and behavioral measurements of early-fattening Hanwoo steers under heat stress conditions.","authors":"Jun Sik Woo, Sun Sik Jang, Jeong Hoon Kim, Hong Gu Lee, Keun Kyu Park","doi":"10.5713/ab.24.0236","DOIUrl":"10.5713/ab.24.0236","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the effects of increased levels of dietary total digestible nutrient (TDN) and crude protein (CP) using protected fat (PF), soybean meal (SB), and heat-treated soybean meal (HSB) on performance, physiological parameters, and behavioral measurements of early-fattening Hanwoo steers under heat stress conditions.</p><p><strong>Methods: </strong>Thirty-six steers (480.9±58.6 kg, 15.9±1.4 months) were assigned to 4 treatments: control (TDN 75%, CP 15%, rumen degradable protein [RDP]:rumen undegradable protein [RUP] = 62:48); PF (TDN 82.5%, CP 15%, RDP:RUP = 62:48); PF+SB (TDN 82.5%, CP 16.5%, RDP:RUP = 62:48); and PF+SB+HSB (TDN 82.5%, CP 16.5%, RDP:RUP = 48:52) for a total of 16 weeks with division of 4 weeks. The average temperature-humidity index (THI) was 82.9 (1st; moderate), 76.9 (2nd; mild), 70.9 (3rd; comfort), and 65.8 (4th period; comfort).</p><p><strong>Results: </strong>Dry matter intake during whole period did not differ among treatments but decreased by 34% and 19%, respectively during 1st and 2nd compared to 4th. Average daily gain (ADG) of PF+SB+HSB was higher (p<0.05) than that of control during the 1st period, and those of both PF+SB and PF+SB+HSB were higher (p<0.05) than Control during the 2nd. The ADG during whole period was in the order of PF+SB+HSB (1.23), PF+SB (1.18), PF (1.11), control (0.98 kg/d) (p<0.05). As THI increased, rectal temperature and all blood parameters increased, while blood glucose levels decreased (p<0.05). Behavioral changes during 1st period compared to 3rd included decreases in lying (24%), walking (48%), and eating (40%), and increases in total standing (50%) and drinking (43%) (p<0.05). Rumination during standing was 38% higher, and rumination during lying was 32% lower (p<0.05).</p><p><strong>Conclusion: </strong>This study demonstrates 10% of increased levels of dietary TDN using PF and CP considering RUP can prevent performance reduction in early-fattening of heatstressed Hanwoo steers and have positive effects on performance recovery from post-heat stress.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"278-292"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary supplementation of xylanase alone or combination of xylanase and β-glucanase on growth performance, meat quality, intestinal measurements, and nutrient utilization in broiler chickens.","authors":"Deok Yun Kim, Kang Hyeon Kim, Eun Cheol Lee, Ju Kyoung Oh, Min Ah Park, Dong Yong Kil","doi":"10.5713/ab.24.0430","DOIUrl":"10.5713/ab.24.0430","url":null,"abstract":"<p><strong>Objective: </strong>The current study aimed to investigate the effect of dietary supplementation of xylanase alone or combination of xylanase and β-glucanase in high non-starch polysaccharides (NSP) diets with low energy on growth performance, meat quality, intestinal measurements, stress responses, and energy and nutrient utilization in broiler chickens.</p><p><strong>Methods: </strong>A total of four hundred 8-d-old Ross 308 broiler chickens were randomly allotted to 1 of 4 treatment groups with 10 replicates. A positive control (PC) diet was formulated with adequate energy and nutrients, whereas a negative control (NC) diet had 100 kcal/kg less nitrogen-corrected apparent metabolizable energy than the PC diet with increasing inclusion of high NSP ingredients. Two additional diets were produced by supplementing 0.1% xylanase alone or 0.1% xylanase and β-glucanase mixture in the NC diet. Experiments lasted for 27 d.</p><p><strong>Results: </strong>Birds fed PC diets had less (p<0.05) feed conversion ratio (FCR) than those fed NC diets. Birds fed NC diets supplemented with xylanase alone or combination of xylanase and β-glucanase had less (p<0.05) FCR than those fed NC diets. Dietary supplementation of xylanase alone in NC diets exhibited the highest (p<0.05) breast meat pH among dietary treatments. Birds fed PC diets or NC diets supplemented with xylanase and β-glucanase combination exhibited greater (p<0.05) villus height:crypt depth ratio than those fed NC diets. Dietary supplementation of xylanase alone and combination of xylanase and β-glucanase in NC diets decreased (p<0.05) ileal digesta viscosity and increased (p<0.05) xylo-oligosaccharide concentrations in the gastrointestinal tract (GIT) compared with NC diets without affecting energy and nutrient utilization in NC diets.</p><p><strong>Conclusion: </strong>Dietary supplementation of xylanase in high NSP diets with low energy concentrations improves growth performance by decreasing digesta viscosity and increasing concentrations of xylo-oligosaccharides in the GIT of broiler chickens. However, little additional benefits of β-glucanase supplementation in combination with xylanase are identified for broiler chickens.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"325-335"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel p.127Val>Ile single nucleotide polymorphism in the MTNR1A gene and its relation to litter size in Thin-tailed Indonesian ewes.","authors":"Mutasem Abuzahra, Mohammed Baqur S Al-Shuhaib, Dwi Wijayanti, Mustofa Helmi Effendi, Imam Mustofa, Ikechukwu Benjamin Moses","doi":"10.5713/ab.24.0187","DOIUrl":"10.5713/ab.24.0187","url":null,"abstract":"<p><strong>Objective: </strong>The primary objective was to identify and characterize the single nucleotide polymorphisms (SNPs) within the MTNR1A gene sequence in Thin-tailed Indonesian ewes to assess the possible association of MTNR1A gene polymorphism with litter size trait.</p><p><strong>Methods: </strong>Forty-seven Thin-tailed Indonesian sheep were selected for the study. Genotyping involved collecting blood samples, and sequencing exon 2 of the MTNR1A gene.</p><p><strong>Results: </strong>The study identified 19 novel SNPs, with 10 being non-synonymous variations, in the MTNR1A gene of Thin-tailed Indonesian ewes. One non-synonymous SNP (rs1087815963) showed a significant association with litter size, with the GC genotype exhibiting a higher average litter size than the GG genotype. The deleterious impact of p.Val127Ile SNP was predicted by various in silico tools that predicted a highly damaging effect of p.Val127Ile SNP on the structure, function, and stability of MTNR1A. Docking reactions showed a critical involvement of this locus with the binding with melatonin.</p><p><strong>Conclusion: </strong>In conclusion, the results of our study suggest that rs1087815963 has a remarkable negative impact on the MTNR1A with a putative alteration in the binding with melatonin. Therefore, the implementation of the novel p.Val127Ile could be a useful marker in marker-assisted selection.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"209-222"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}