Animal Bioscience最新文献

{"title":"Assessment of genetic diversity and inbreeding in the Okinawa indigenous Agu pig through pedigree analysis.","authors":"Shihei Touma, Kyota Fusho, Aisaku Arakawa","doi":"10.5713/ab.24.0646","DOIUrl":"10.5713/ab.24.0646","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to clarify the changes in genetic diversity and inbreeding over the years as well as to identify the causes of genetic diversity loss using the pedigree of Okinawa indigenous Agu pigs.</p><p><strong>Methods: </strong>The pedigree of the Agu population consisted of 1,997 animals from 1997 to 2020. The equivalent complete generations (ECG), inbreeding coefficient (F), and effective population size (Ne) were computed using ENDOG v4.8. Genetic diversity parameters, including founders (f), the effective number of founders (fe), the effective number of ancestors (fa), and the founder genome equivalent (fge), were derived from the probability of gene origin. The study also investigated changes in genetic diversity indices over time.</p><p><strong>Results: </strong>ECG increased from 0.1 in 2003 to 3.87 by 2020, indicating an improved pedigree depth. The F has been on a downward trend since it peaked at 10.5% in 2015. However, the Ne in the reference population was 14.6, which is below the recommended levels. The f, fe, fa and fge of the reference population were 86, 26, 19, and 11.5, respectively. These values decrease in the order of f>fe>fa>fge. This indicates that all factors of genetic diversity loss played a role, including the unbalanced contributions of founders, bottlenecks, and random gene losses. Although in recent years efforts have been made to maintain genetic diversity and reduce inbreeding, the Agu pig population exhibited a 5% decrease in genetic diversity over the past 18 years, with both unequal founder contributions and genetic drift identified as significant contributing factors.</p><p><strong>Conclusion: </strong>This study reveals that more favorable breeding strategies (such as optimal contribution and population size expansion) are required to maintain genetic diversity and increase the Ne of the Agu pig population.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1105-1115"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a multi-strain probiotic on productive traits, antioxidant defence, caecal microbiota and short‑chain fatty acid profile, and intestinal histomorphology in rabbits.","authors":"Vincenzo Tufarelli, Caterina Losacco, Gianluca Pugliese, Alessandra Tateo, Michele Schiavitto, Fabrizio Iarussi, Vito Laudadio, Letizia Passantino","doi":"10.5713/ab.24.0716","DOIUrl":"10.5713/ab.24.0716","url":null,"abstract":"<p><strong>Objective: </strong>This paper aimed to evaluate the effects of a multi-strain probiotic (MS-Prob) as natural feed additives on the productive performance, blood parameters, antioxidant defence, caecal short-chain fatty acid profile as well as the effectiveness on the intestinal morphology and on the equilibrium modification of caecal microbiota of growing rabbits.</p><p><strong>Methods: </strong>Eighty-six-week-old Italian White rabbits were assigned into two dietary groups: the control group was fed without any additive, while the test group received a diet supplemented with a MS-Prob (Slab51) at a dosage of 250 mg/kg diet. The feeding trial lasted up to 12 weeks of age.</p><p><strong>Results: </strong>Rabbits MS-Prob diet had significantly higher daily and final body weight recording also the best of feed efficiency compared to control group. Rabbits in MS-Prob group showed improved slaughter weight and carcass dressing yield. No significant effect was found on meat protein, lipids and ash contents. Serum total cholesterol, low-density lipoprotein, high-density lipoprotein and triglycerides decreased significantly in rabbits fed the test-diet. In rabbits fed MS-Prob, the activity of glutathione peroxidase, glutathione S-transferase, catalase and superoxide dismutase increased significantly, whereas the level of thiobarbituric acid-reactive substance decreased significantly. Caecal pH, ammonia-N and total volatile fatty acids (VFAs) were not significantly influenced by dietary treatments. Conversely, VFAs molar proportions were statistically affected by diets, with higher acetic and butyric acid concentrations in caecum of rabbits fed MS-Prob. Feeding of MS-Prob reduced harmful bacterial growth (Escherichia coli, Bacillus spp., Clostridium spp.) while promoting beneficial bacteria (Lactobacillus spp., Bacteroides spp.). Moreover, MS-Prob positively influences rabbit gut histomorphology, showing higher villus height, villus width, and crypt depth.</p><p><strong>Conclusion: </strong>This study indicated that MS-Prob (Slab51) supplementation stimulated the performance of growing rabbits and positively affected blood serum parameters, intestinal morphology, and caecal environment and microbiota.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1247-1258"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of hormonal treatments on progesterone levels to enhance embryo survival and kidding rates in goats.","authors":"Manita Wittayarat, Navapol Kupthammasan, Hakim Jehdo, Ratree Jintana, Sopita Suttikrai, Niraporn Tongkumtae, Nantarat Chutijiratthitkan, Pokchon Khirilak, Sirirat Norsoongnern, Supitcha Kaewma, Chaiyawan Wattanachant, Saritvich Panyaboriban","doi":"10.5713/ab.24.0578","DOIUrl":"10.5713/ab.24.0578","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the efficiency of different estrous synchronization programs and hormonal treatments in improving reproductive outcomes in goats. Conducted on a commercial farm in southern Thailand, the study used multiparous Shami and Anglo-Nubian breed goats.</p><p><strong>Methods: </strong>In experiment 1, goats were randomly allocated to two estrous synchronization treatments: 11-day (CI11D) and 13-day (CI13D) intravaginal progesterone implants, followed by artificial insemination (AI) with frozen-thawed semen. Various hormonal treatments (human chorionic gonadotropin [hCG], gonadotropin-releasing hormone [GnRH] analogue, progestogen) were administered on day 5 post-AI to elevate progesterone levels. Experiment 2 assessed embryo survival after transferring early- and late-stage embryos, using GnRH analogue to increase progesterone levels in the recipient goats.</p><p><strong>Results: </strong>Results showed that GnRH analogue significantly increased pregnancy rates, kidding rates, and the number of kids born in the CI13D group. Progesterone levels were higher in treated groups, particularly with GnRH analogue, though estradiol levels varied between synchronization protocols. Late-stage embryo transfers significantly improved pregnancy rates and reduced embryonic loss compared to early-stage transfers. GnRH analogue supplementation reduced early embryonic mortality, enhancing embryo survival and overall reproductive performance.</p><p><strong>Conclusion: </strong>This study demonstrates the efficacy of tailored estrous synchronization and hormonal treatments in optimizing goat reproductive outcomes, with significant implications for improving fertility management in commercial goat farming. Although no significant differences were observed in progesterone levels, the administration of GnRH analogue reduced early embryonic mortality and improved reproductive outcomes, demonstrating its potential to enhance embryo survival and reproductive performance in goats.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1140-1149"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal dynamics of plasma bile acids and their associations with physiological parameters and fecal microbiome during the transition period in dairy cows.","authors":"Feixiang Fan, Liang Chen, Huizeng Sun, Jianxin Liu, Kailun Yang, Fengfei Gu","doi":"10.5713/ab.24.0628","DOIUrl":"10.5713/ab.24.0628","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study is to investigate the dynamic changes of plasma bile acids (BA) and their correlations with physiological parameters and fecal microbiome in transitional dairy cows.</p><p><strong>Methods: </strong>Twenty multiparous dairy cows were selected, the blood and fecal samples were collected on d -21, -7, +7, and +21 of calving. The targeted metabolome and 16s rDNA ampicon sequencing were utilized to identify BA profiles and fecal microbial composition, respectively.</p><p><strong>Results: </strong>A total of 32 BAs were found, comprising 9 primary BAs (PBA) and 23 secondary BAs (SBA). Majority of the PBAs (7 out to 9) and SBAs (15 out to 23) exhibited significant increases postpartum compared to prepartum levels. Notably, ursodeoxycholic acid, taurocholic acid and 7-ketodeoxycholic acid showed higher importance. Correlation analysis showed the BAs concentrations positively correlated with the concentrations of aspartate aminotransferase, total antioxidant capacity, and glutathione peroxidase, while exhibiting substantial negative correlation with triglyceride concentrations. A decline in bacterial alpha diversity in postpartum and significantly different β-diversity were observed. Furthermore, 30 distingtive genera were identified over the transition period. Among these, six and eleven biomarkers such as Alistipes and Ruminococcaceae_UCG_014 were identified at +7 d and +21 d, respectively. Furthermore, the abundances of choloylglycine hydrolase and 7-alpha-hydroxysteroid dehydrogenase which are involved in SBA biosynthesis were significantly higher postpartum as determined by PICRUSt2 analysis over the transition period.</p><p><strong>Conclusion: </strong>The BA profile and concentrations underwent significant changes during the transition period in dairy cows and these changes are closely related to the periparturient health of the cows. Ursodeoxycholic acid and Alistipes was identified as the pivotal BA and microbial genus. Our study elucidates these metabolic processes, providing useful insights into strategies for enhancing the nutrition and well-being of perinatal dairy cows.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1194-1205"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Milk profiles of selenoamino acids, selenoenzymes, and selenoproteins of peripartal dairy cows fed with different selenium sources.","authors":"Xiaoyu Ji, Xuejuan Deng, Ning Liu, Jianping Wang","doi":"10.5713/ab.24.0571","DOIUrl":"10.5713/ab.24.0571","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the effect of dietary nanoselenium (nanoSe) and sodium selenite (SS) on the concentrations of selenoamino acids, the activities of selenoenzymes, and the mRNA expressions of selenoproteins in the milk of peripartal dairy cows.</p><p><strong>Methods: </strong>Three diets included a control (basal diet) with background selenium at 0.06 mg/kg and treatments with either SS or nanoSe added at the same selenium concentration of 1.00 mg/kg of diet. A total of 45 dairy cows were randomly allocated to three groups. The feeding trial lasted for 42 days from prenatal 21 days to postnatal 21 days.</p><p><strong>Results: </strong>NanoSe increased (p<0.05) milk yield compared to the control and SS. In milk, both SS and nanoSe increased (p<0.05) the concentrations of selenium, selenocysteine, and selenomethionine, and the activities of glutathione peroxidase 4, glutathione reductase, thioredoxin reductase, glutathione S-transferase, and iodothyronine deiodinases (type 2 and 3). The nanoSe showed higher (p<0.05) effects on these parameters than SS. Also, dietary nanoSe upregulated (p<0.05) the mRNA expressions of selenoproteins P, W, S, F, M, N, K, O, H, and I in milk compared to the control. For most selenoproteins, there was no difference between SS and nanoSe, only selenoprotein K was higher (p<0.05) in nanoSe than in SS.</p><p><strong>Conclusion: </strong>In conclusion, dietary nanoSe increased milk yield, milk selenoamino acids and selenoproteins in peripartal dairy cows.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1175-1181"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of calcium lactate on in vitro fertilization and embryonic development in cattle.","authors":"Bo-Myeong Kim, Song-Hee Lee, Geun Heo, Ji-Dam Kim, Gyu-Hyun Lee, Jae-Min Sim, Kwang Taek Lim, Xiang-Shun Cui","doi":"10.5713/ab.24.0636","DOIUrl":"10.5713/ab.24.0636","url":null,"abstract":"<p><strong>Objective: </strong>Growing demand for embryo transfer is steadily expanding and further studies on in vitro fertilization of cattle. To assess the effect of calcium lactate by replacing Tyrode's albumin lactate pyruvate (TALP) medium composition during fertilization and embryonic development.</p><p><strong>Methods: </strong>Sodium lactate and CaCl2 were replaced with 2.0, 3.0, 4.5 mM calcium lactate for TALP medium during fertilization in Experiment 1. In Experiment 2, the concentrations of sodium lactate and CaCl2 were re-modified as control, in comparison with the same concentration of calcium lactate at 4.5 mM. Zygotes were moved to sequential media to match early-and late-stage environments. Embryonic development was examined on day 8 after insemination.</p><p><strong>Results: </strong>A 4.5 mM calcium lactate enhanced the rate of fertilization and blastocyst formation (p<0.0001, p<0.01, respectively). It represented differences in the reactive oxygen species (ROS) (p<0.01) and glutathione (GSH) levels (p<0.05) and increased blastocyst diameter and total cell number (p<0.05). In Experiment 2, fertilization (p<0.05) and blastocyst formation rates (p<0.01) were increased in 4.5mM calcium lactate under same concentration effect of sodium lactate and CaCl2. Additionally, it reduced the ROS (p<0.01) and increased the GSH levels (p<0.05), leading increase embryo quality.</p><p><strong>Conclusion: </strong>The replacement of calcium lactate in TALP medium enhances fertilization and embryonic development while also improving oxidative stress. Specifically, it has been determined that a concentration of 4.5 mM calcium lactate is the most effective, irrespective of the varying concentrations of sodium lactate and CaCl2. This study presents a novel formulation of a modified TALP medium intended for implantation withing the bovine embryo industry. The current implications of the study are discussed in relation to previously stated objectives and hypotheses.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1150-1159"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061575/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination and prediction of net energy of soybean meal fed to pregnant sows by indirect calorimetry.","authors":"Lei Xue, Can Zhang, Bo Cheng, Qian Song, Lee J Johnston, Ling Liu, Fenglai Wang, Jianjun Zang","doi":"10.5713/ab.24.0663","DOIUrl":"10.5713/ab.24.0663","url":null,"abstract":"<p><strong>Objective: </strong>The study was conducted to investigate the appropriate substitution level of soybean meal (SBM) for determining its net energy (NE), and establish NE prediction equation of SBM based on the determined NE values for pregnant sows.</p><p><strong>Methods: </strong>In Exp. 1, eighteen pregnant sows (Landrace×Yorkshire; parity, 2 to 3) with an initial body weight (BW) of 221.2±2.6 kg at mid-gestation were blocked by BW and randomly assigned into 3 groups. Three groups fed with a corn-SBM basal diet and two test diets with 15% and 30% energy-supplying components replaced by SBM, respectively. In Exp. 2, six diets were formulated including a corn-SBM basal diet and five SBM diets (based on the substitution level determined of Exp. 1) with different soybean sources and processing methods. Moreover, 12 pregnant pigs (BW = 209.0±3.0 kg; parity, 3 to 4) at mid-gestation were arranged in a 6×3 Youden square design.</p><p><strong>Results: </strong>Increasing substitution levels of SBM linearly increased (p<0.05) fecal and urinary nitrogen excretion and the ratio of urinary energy to digestible energy (DE), while linearly decreased (p<0.05) the ratio of metabolizable energy (ME) to DE and tended to linearly decrease dietary ME (p = 0.066) and NE (p = 0.074). The coefficient of variation for the NE of SBM was lower at a 15% substitution level compared to a 30% substitution level. The nutritional compositions of SBM are influenced by the soybean sources and processing methods. As dry matter basis, NE values of SBM ranged from 11.1 to 12.7 MJ/kg and the best-fitted prediction equation for NE of SBM was: NE (MJ/kg) = -91.71+5.35×gross energy (%)-0.03×neutral detergent fiber (%; R2 = 0.96).</p><p><strong>Conclusion: </strong>A substitution level of 15% was more appropriate to determine NE of SBM. Furthermore, NE values of SBM can be predicted based on their chemical compositions.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1228-1241"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative and comparative analysis of whole-transcriptome sequencing in circCOL1A1-knockdown and circCOL1A1-overexpressing goat hair follicle stem cells.","authors":"Jian Wang, Xi Wu, Liuming Zhang, Xiaomei Sun, Wei Sun, Kunzhe Dong, Yongjun Li","doi":"10.5713/ab.24.0816","DOIUrl":"10.5713/ab.24.0816","url":null,"abstract":"<p><strong>Objective: </strong>Hair morphogenesis is tightly related to hair follicle stem cells (HFSCs) proliferation and hair follicle (HF) development. Yangtze River Delta white goats (YRDWG) HFSCs are important for producing superior-quality brush hair (SQBH). Nonetheless, the known regulatory mechanisms are not sufficient to explain YRDWG gHFSCs growth, HF development, and SQBH formation.</p><p><strong>Methods: </strong>To deeply investigate the interaction networks and mechanisms of circCOL1A1 in the HF development and SQBH formation of YRDWG in detail, we applied whole-transcriptome sequencing and bioinformatics analysis of circCOL1A1-knockdown and circCOL1A1-overexpressing HFSCs from YRDWG. STRING and other databases were used to construct multiple interaction networks. Differentially expressed (DE) genes, DE-miRNAs, and DE-circRNAs were further confirmed via real-time quantitative polymerase chain reaction and Sanger sequencing.</p><p><strong>Results: </strong>A total of 87 genes, 96 miRNAs, and 135 circRNAs were DE between circCOL1A1-knockdown and circCOL1A1-overexpressing gHFSCs. Functional enrichment, gene ontology annotation and Kyoto encyclopedia of genes and genomes analyses identified marked enrichment of these DE- genes, DE-miRNAs, and DE-circRNAs in the MAPK, PI3K/Akt, and focal adhesion signaling pathways, which are closely associated with gHFSCs growth and HF development. In addition, through interaction network construction, four important regulatory axes were obtained, namely, the chi-circCOL1A1-miR-149-5p-CMTM3-AR, chi-circACTN1- miR-671-5p-MAPK3/COL13A1, chi-circITGA6-miR-18a-5p-FGF1/MAP3K1 and chi-circ COBLL1-miR-30a-5p/miR-128-3p-ITGA6/MAPK14/FGF14 axes.</p><p><strong>Conclusion: </strong>These novel findings provide a valuable and comprehensive basis for investigating the complex mechanism by which circRNAs participate in and regulate HF development and SQBH formation in YRDWG.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1116-1139"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-21-5p enhances differentiation and mitigates oleic acid-induced lipid droplet accumulation in C2C12 myoblasts by targeting FBXO11.","authors":"Yi Li, Jiayuan Fang, Yingying Jiao, Qinchuan Lv, Xingyu Xiao, Shuo Zheng, Xi Chen, Jie Song, Xunming Zhang, Libo Zhang, Ze Ma, Changhong Li, Linlin Hao","doi":"10.5713/ab.24.0665","DOIUrl":"10.5713/ab.24.0665","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the role of miRNA 21-5p in regulating the differentiation of C2C12 myoblasts and intramuscular lipid droplets accumulation in myotubes.</p><p><strong>Methods: </strong>The role of miR-21-5p in the proliferation and differentiation of myofibroblasts and intracellular lipid accumulation was analyzed using bioinformatics, CCK-8 assay, quantitative real-time polymerase chain reaction, immunoblotting, immunofluorescence staining, and Oil Red O staining.</p><p><strong>Results: </strong>The analysis of porcine BodyMap transcriptome data revealed differential expression of miRNA 21-5p in skeletal muscle and adipose tissue. Bioinformatics analysis combined with a dual-luciferase reporter assay demonstrated that FBXO11 serves as a direct target of miR-21-5p. Transfection experiments involving a miR-21-5p mimic, miR-21-5p inhibitor, and si-FBXO11 in C2C12 cells showed that overexpression of miR-21-5p or silencing of FBXO11 significantly enhanced the proliferation of C2C12 cells, upregulated myogenesis-related factors, and promoted myotube formation. Furthermore, oleic acid-induced lipid accumulation in myotubes was suppressed, accompanied by reduced expression of adipogenesis-related genes. Conversely, inhibition of miR-21-5p expression produced opposite effects.</p><p><strong>Conclusion: </strong>These findings indicate that miR-21-5p promotes proliferation and differentiation while inhibiting intramyocellular lipid deposition by targeting the 3'-untranslated region of FBXO11 in myogenic cell. The results suggest that miR-21-5p could serve as a potential miRNA biomarker for regulating intramuscular adipogenesis.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1279-1290"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary rumen undegradable protein:rumen degradable protein ratio on nitrogen metabolism in Hanwoo steers.","authors":"Sang Yeob Kim, Rajaraman Bharanidharan, Seyun Im, Kyoung Hoon Kim, Joonpyo Oh, Hyun Jin Kim, Jaesung Lee, Kamburawala Kankanamge Tharindu Namal Ranaweera, Jin Woo Jeong, Jun Seok Oh, Sang Hyun Lee, Myunggi Baik","doi":"10.5713/ab.24.0592","DOIUrl":"10.5713/ab.24.0592","url":null,"abstract":"<p><strong>Objective: </strong>We investigated the effects of dietary rumen undegradable protein (RUP): rumen degradable protein (RDP) ratio on growth performance, nitrogen (N) metabolism, and rumen and blood parameters in Hanwoo (Korean cattle) steers.</p><p><strong>Methods: </strong>Eight Hanwoo steers (average body weight, 393 kg) were allocated to two groups and fed with a high RUP:RDP ratio (46.9:53.1 based on crude protein) or a low RUP:RDP ratio (30.6:69.4) concentrate with iso-crude protein content in a 2×2 Latin square design in two successive periods.</p><p><strong>Results: </strong>The high RUP:RDP group had higher (p<0.01) average daily gain, and lower (p<0.05) ruminal ammonia and plasma urea N concentrations than the low RUP:RDP group. The high RUP:RDP group had lower (p<0.05) urinary N excretion and urinary N per N intake and higher (p<0.1) tendency of retained N than the low RUP:RDP group. The high RUP:RDP group had higher (p<0.1) tendency of N utilization efficiency (retained N per N intake: 28.7% vs. 25.5%) than the low RUP:RDP group. The high RUP:RDP group had a lower (p<0.1) tendency of urinary N per total N excretion, but a higher tendency of fecal N per total N excretion.</p><p><strong>Conclusion: </strong>A high ratio of dietary RUP:RDP may improve N utilization efficiency by reducing urinary N excretion, which may be beneficial for the environment through reducing atmospheric ammonia emissions.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1182-1193"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}