Animal Bioscience最新文献

{"title":"Plant bioactive compounds as functional feed additives for improving livestock and poultry production: review.","authors":"Do Thi Cat Tuong, Adhimoolam Karthikeyan, Lin Lat La Min, Sureshbabu Anjana, Masayuki Funaba, Sanggun Roh, Taesun Min","doi":"10.5713/ab.250961","DOIUrl":"10.5713/ab.250961","url":null,"abstract":"<p><p>The administration of antibiotics to enhance growth and prevent diseases in livestock and poultry has elicited concerns over antimicrobial resistance and its implications for public health. The escalating demands on the livestock and poultry sectors necessitate enhanced productivity, diminished economic losses, and the guarantee of food safety for human consumption. Plant bioactive compounds (PBCs) have developed as safe and sustainable alternatives to antibiotics that enhance growth, bolster animal health, and boost productivity without jeopardizing food safety. PBCs represent a diverse group of secondary metabolites-including phenolics, terpenoids, polysaccharides, and organosulfur compounds-that exhibit a wide range of biological activities relevant to livestock and poultry nutrition and health. Recent studies have shown that supplementing animals with PBCs as feed additives, either as crude extracts or individual compounds, improves growth performance, nutrient utilization and modulates gut microbiota, while exerting antioxidant, anti-inflammatory, antimicrobial, and immunoregulatory effects, and thereby enhancing overall resilience of animals. The multifunctional properties of PBCs, along with the reduced risk of resistance development, position them as promising candidates for next-generation feed additives for livestock and poultry production. However, challenges remain regarding optimal concentration, variability in bioavailability, stability during feed processing, and the standardization of active components. Here, we discuss the major PBCs and their potential as functional feed additives to improve livestock and poultry production. We further highlight research gaps and outline future prospects required to promote their integration into sustainable animal production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"250961"},"PeriodicalIF":3.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148315294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of fatty acids, amino acids, and flavor compounds in different skeletal muscles of the Tianzhu white yak.","authors":"Xiangyan Wang, Youpeng Qi, Changze Cui, Shaopeng Chen, Zhihao Luo, Meixian Zhang, Yiduo Dai, Yanbin Zhu, Jiang Hu, Bingang Shi, Shaobin Li","doi":"10.5713/ab.250878","DOIUrl":"10.5713/ab.250878","url":null,"abstract":"<p><strong>Objective: </strong>Although yak meat is valued for its distinctive flavor profile, little is known about the mechanisms underlying flavor variation among different muscle types. This study aimed to systematically investigate the differences in flavor precursors, metabolic enzyme activities, gene expression profiles, and volatile flavor compounds among three muscles-longissimus thoracis (LT), triceps brachii (TB), and semimembranosus (SM) of the Tianzhu white yak, and to elucidate the underlying regulatory mechanisms.</p><p><strong>Methods: </strong>A comprehensive approach was employed, integrating targeted metabolomics, untargeted metabolomics, flavoromics, real-time quantitative polymerase chain reaction, and histochemical staining to compare the biochemical characteristics of the three muscles.</p><p><strong>Results: </strong>The results of volatile profiles and sensory evaluation demonstrated that the TB muscle exhibited more favorable flavor-related traits. The TB muscle was enriched in key flavor precursors, including glutamic acid, glutamine, arginine, and total free amino acid content (p<0.05). Concurrently, the expression levels of key lipogenic genes (FASN, ELOVL4, SREBP1) were significantly higher in the TB muscle (p<0.05). Flavoromics analysis further revealed that the TB muscle exhibited greater diversity and higher relative abundance of key volatile compounds, such as aldehydes, ketones, and alcohols (p<0.05). Sensory evaluation further indicated that the TB muscle exhibited stronger sweet and fruity flavors. Untargeted metabolomics and pathway enrichment analysis indicated that this profile was associated with enrichment of arginine and proline metabolism and unsaturated fatty acid biosynthesis.</p><p><strong>Conclusion: </strong>This study demonstrates that flavor enhancement in yak meat is associated with the coordinated upregulation of lipid synthesis genes, thereby promoting the accumulation of flavor precursors. This process provides sufficient substrates for the Maillard reaction and lipid oxidation, facilitating the generation of volatile flavor compounds. These findings provide a theoretical basis for yak meat quality evaluation and precision processing.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"250878"},"PeriodicalIF":3.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147607481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An in vitro ileal disappearance procedure for estimating ileal digestibility of protein in weanling pigs.","authors":"Yujeong Hwang, Yoon Soo Song, Beob Gyun Kim","doi":"10.5713/ab.260517","DOIUrl":"https://doi.org/10.5713/ab.260517","url":null,"abstract":"<p><strong>Objective: </strong>The present experiment aimed to develop an in vitro procedure for estimating standardized ileal digestibility (SID) of crude protein (CP) in feed ingredients for weanling pigs by modifying the conventional procedure for growing pigs.</p><p><strong>Methods: </strong>In the conventional in vitro ileal disappearance (IVID) procedure, sample solutions added with 1 mL of pepsin (10.0 g/L) are incubated for 6.0 h in step 1 to simulate digestion in the stomach followed by adding 1 mL of pancreatin (50.0 g/L) and incubating for 18.0 h in step 2 to simulate digestion in the small intestine. To develop an IVID procedure for weanling pigs, 3 incubation periods (6.0, 4.5, and 3.0 h in step 1 and 18.0, 13.5, and 9.0 h in step 2) and 3 enzyme concentrations (pepsin 10.0, 7.5, and 5.0 g/L in step 1 and pancreatin 50.0, 37.5, and 25.0 g/L in step 2) were tested in a 3×3 factorial arrangement. Test ingredients were rice, corn, wheat, rapeseed meal, soybean meal, fermented soybean meal, and soy protein concentrate.</p><p><strong>Results: </strong>The IVID of CP measured using the conventional procedure deviated from the weanling pig's in vivo SID of CP by 5.0% on average. Based on the quadratic response surface analysis, the use of 1 mL of pepsin at 7.6 g/L with the incubation period of 4.4 h in step 1 followed by adding 1 mL of pancreatin at 38.1 g/L with the incubation period of 13.3 h in step 2 minimized the absolute difference between IVID of CP and in vivo SID of CP to 3.6% on average for weanling pigs.</p><p><strong>Conclusion: </strong>The in vitro procedure for weanling pigs was developed by lowering the incubation periods and enzyme concentrations for estimating ileal digestibility of protein in feed ingredients with greater accuracy compared with the conventional in vitro procedure.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant properties of kiwifruit cultivars and their effects on oxidative stability of chicken breast under overwrap and modified atmosphere packaging.","authors":"Ha Eun Kim, Koo Bok Chin","doi":"10.5713/ab.260359","DOIUrl":"https://doi.org/10.5713/ab.260359","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the antioxidant properties of red kiwifruit (RK), green kiwifruit (GRK), and gold kiwifruit (GOK) powders and evaluated their effects on oxidative stability of chicken breast stored under overwrap packaging (OWP) or modified atmosphere packaging (MAP).</p><p><strong>Methods: </strong>Kiwifruit powders prepared from red, green, and gold kiwifruit cultivars were analyzed for total phenolic content and antioxidant activity, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power. Chicken breasts were injected with brine solutions containing kiwifruit powder, ascorbic acid (AA), or plain brine as a control (CTL), and stored under overwrap packaging (OWP) or modified atmosphere packaging (MAP) at 10 °C for 9 days. Thiobarbituric acid reactive substances (TBARS) and volatile basic nitrogen (VBN) were measured during storage. Statistical analysis was performed using one-way and two-way analysis of variance.</p><p><strong>Results: </strong>GOK exhibited the highest total phenolic content and antioxidant activity. Under OWP, TBARS values were lower in AA, RK, and GOK than in CTL and GRK. For VBN, GOK showed a value comparable to that of AA under OWP.</p><p><strong>Conclusion: </strong>Oven-dried gold kiwifruit powder showed the highest antioxidant activity among the tested kiwifruit cultivars and was effective in improving the oxidative stability of chicken breast, particularly under overwrap packaging.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deer antler peptides attenuate inflammation, oxidative stress, and pyroptosis in Salmonella-infected macrophages and murine colonic tissues.","authors":"Deshou Zeng, Haihua Xing, Zihui Sun, Yukai Ma, Qing Wang, Fuquan Yin, Zhenhua Gao, Heping Li","doi":"10.5713/ab.260612","DOIUrl":"https://doi.org/10.5713/ab.260612","url":null,"abstract":"<p><strong>Objectives: </strong>Deer antler peptides (DAPs), have been widely reported to exhibit potent anti-inflammatory and antioxidant properties, their efficacy in alleviating pathogen-induced intestinal infections mechanisms remain poorly understood. In this study, we investigated the protective effects of DAPs against Salmonella-induced intestinal inflammation and associated tissue injury and elucidated the molecular mechanisms underlying these processes.</p><p><strong>Methods: </strong>Primary peritoneal macrophages isolated from wild-type (WT) and NRF2 knockout (nrf2-/-) mice were infected with Salmonella; concurrently, we established a murine model of Salmonella-induced intestinal inflammation by infecting WT and nrf2-/- mice. Using in vitro and in vivo systems, we evaluated the immunomodulatory effects of DAPs during Salmonella infection.</p><p><strong>Results: </strong>In vitro, the results demonstrated that DAPs significantly inhibited the Salmonella-induced production of TNF-α and IL-6, the activation of MAPK signaling pathway, GSDMD-mediated pyroptosis, and excessive ROS accumulation. Mechanistically, DAPs activate the NRF2 signaling pathway by promoting nuclear translocation and the transcriptional upregulation of HO-1 and NQO-1. The genetic deletion of NRF2 substantially attenuated the protective effects of DAPs against inflammation, pyroptosis, and oxidative stress, confirming the mechanism's dependency on this pathway. In vivo, DAPs significantly reduced intestinal histopathological changes, epithelial barrier disruption, pyroptosis, and oxidative damage in WT mice, while these protective effects were markedly diminished in nrf2-/- mice. Notably, DAPs significantly reduced colonic bacterial loads in both WT and nrf2-/- mice while concurrently suppressing pyroptosis. Our findings indicate that DAPs may exert antibacterial effects through a mechanism independent of both NRF2 activation and the NLRP3-mediated pyroptotic pathway; however, the underlying molecular mechanism remains to be fully elucidated.</p><p><strong>Conclusion: </strong>Our findings identify DAPs as multi-modal regulators that coordinately suppress inflammation, pyroptosis, and oxidative stress through NRF2 activation during Salmonella infection, and provides mechanistic insights and a translational basis for the application of animal-derived peptides in the treatment of infectious intestinal disorders.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stage-specific prediction of body weight in Hanwoo steers using body measurements and nutrient intake variables.","authors":"Myungsun Park, Sun-Sik Jang, Youl-Chang Baek","doi":"10.5713/ab.260453","DOIUrl":"https://doi.org/10.5713/ab.260453","url":null,"abstract":"<p><strong>Objective: </strong>Accurate body weight (BW) prediction is important for growth management and nutrient utilization in Hanwoo steers. However, prediction performance may vary across growth stages and input variables. Therefore, this study evaluated stage-specific machine learning models for BW prediction using body measurements and nutrient intake variables.</p><p><strong>Methods: </strong>Data from 136 Hanwoo steers were collected during the growing (6-12 months), early fattening (13-21 months), and late fattening (22-31 months) periods. Body size traits and nutrient intake variables were used to develop prediction models using Random Forest (RF), Linear Regression (LR), K-Nearest Neighbors (KNN), and Artificial Neural Networks (ANN). To minimize repeated-measurement bias, datasets were divided into training and testing sets based on individual animal identification. Prediction performance was evaluated using coefficient of determination (R²), root mean square error (RMSE), and mean absolute error (MAE).</p><p><strong>Results: </strong>BW showed strong associations with skeletal-related body traits during the growing period, whereas nutrient intake variables became more strongly associated with average daily gain (ADG) during fattening. Among the evaluated ML models, RF generally demonstrated robust predictive performance across growth stages. For BW prediction, RF achieved the highest accuracy during the growing period (R² = 0.952), whereas LR achieved the highest accuracy during the early-fattening period (R² = 0.947). ADG prediction performance was generally lower than that for BW. Variable importance analysis indicated that skeletal-related body traits contributed most to BW prediction, whereas nutrient intake variables contributed more to ADG prediction during fattening.</p><p><strong>Conclusion: </strong>Factors associated with BW and ADG differed according to production stage. Skeletal-related body traits were more strongly associated with BW during the growing period, whereas nutrient intake variables became increasingly important during fattening. Integrating body measurements and nutritional information may support stage-specific growth monitoring and provide preliminary information for nutritional management in Hanwoo production systems.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of exogenous glucoamylase on ruminal in situ and in vitro dry matter and starch degradability of cereal grains in beef cattle.","authors":"Lin Mu, Kleves V Almeida, Carlos A NinodeGuzman, Ignacio F Marenchino, Kathy G Arriola, Halima Sultana, Nicolas DiLorenzo, Wenting Li, Diwakar Vyas","doi":"10.5713/ab.25.0328","DOIUrl":"10.5713/ab.25.0328","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effects of glucoamylase on ruminal in situ and in vitro dry matter degradability (IVDMD), in vitro starch degradability (IVSD), volatile fatty acids (VFA), gas production and methane production using cereal grains as substrates in beef cattle.</p><p><strong>Methods: </strong>Six substrates (4 mm; 0.70 g per F57 bag) including sorghum (micronized and whole), barley (whole and steam-flaked), and corn (dry-rolled and steam-flaked); were incubated with exogenous glucoamylase (from Trichoderma reesei; 0.25 mg/g substrate dry matter [DM]) and buffered rumen fluid in six replicates per run in 3 independent runs. IVDMD, IVSD, in situ DMD (ISDMD), VFA, and methane production was measured after 7, and 12 h of incubation. ISDMD was measured after 0, 1, 3, 7, and 12 h of ruminal incubation in beef cattle fed high grain diets.</p><p><strong>Results: </strong>Exogenous glucoamylase increased IVDMD and gas production for all substrates except whole sorghum and barley while ISDMD was increased for all substrates except whole barley. Glucoamylase increased IVSD for steam flaked barley and corn. Glucoamylase supplementation increased molar proportion of propionate and decreased acetate-to-propionate ratio, regardless of substrates used. Similarly, glucoamylase supplementation increased methane production with steam-flaked corn.</p><p><strong>Conclusion: </strong>Glucoamylase supplementation can potentially improve DM and starch degradability in cereal grains commonly used as ingredients in beef cattle diets.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"250328"},"PeriodicalIF":3.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145353387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating rumen microbiome and host metabolome to investigate feed conversion ratio across different fattening stages in Hu sheep.","authors":"Quanzhong Xu, Xiaoxue Zhang, Huibin Tian, Xiaobin Yang, Jian Zhang, Hongjian Li, Zongwu Ma, Deyin Zhang, Kai Huang, Yukun Zhang, Yuan Zhao, Xiaolong Li, Liming Zhao, Jiangbo Cheng, Dan Xu, Fadi Li, Xiuxiu Weng, Weiwei Wu, Weimin Wang","doi":"10.5713/ab.260317","DOIUrl":"10.5713/ab.260317","url":null,"abstract":"<p><strong>Objective: </strong>Feed conversion ratio (FCR) is a crucial economic trait in animal breeding and management and is also important for environmental sustainability. This study aimed to investigate the putative regulatory mechanisms of FCR in sheep by integrating rumen microbiota and host metabolome through multi-omics analysis.</p><p><strong>Methods: </strong>FCR data were collected from 127 male Hu sheep. Extreme individuals were selected for rumen metagenomic and serum metabolomic analyses to identify key factors driving FCR across early and late fattening stages.</p><p><strong>Results: </strong>Bacteroides, Prevotella, and other genera were identified as dominant taxa in the rumen across both stages, suggesting their ipotential role in FCR regulation. Notably, Nocardia tengcongensis differed significantly between the highest FCR values (HF) and lowest FCR values (LF) groups at different stages, indicating its potential as a predictive biomarker of feed efficiency. Functional analysis revealed that the pentose phosphate pathway (M00004) and lysine biosynthesis via the succinyl-DAP pathway (M00016) were enriched in the LF group, whereas the methanogenesis pathway (M00357) was significantly enriched in the HF group, indicating increased methane production. Thirteen metabolites consistently differed between HF and LF across fattening stages and they may be used as predictive biomarkers. Furthermore, the abundance of Prevotella and Bacteroides increased over time and showed significant correlations with key metabolites.</p><p><strong>Conclusion: </strong>These findings suggest strong interactions between rumen microbiota and host metabolites that may collectively influence FCR, providing new insights into microbial and metabolic regulation of feed efficiency and a theoretical basis for optimizing feeding strategies in sheep.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"260317"},"PeriodicalIF":3.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148142267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ruminal methanogen abundance and associated physiological characteristics in Japanese Black-Holstein F1 calves.","authors":"Huseong Lee, Yuta Suetomi, Satoshi Haga, Sanggun Roh","doi":"10.5713/ab.260205","DOIUrl":"10.5713/ab.260205","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated associations among ruminal total methanogen abundance, rumen microbiota, fermentation characteristics, feed intake, growth performance, and blood biochemical parameters in Japanese Black-Holstein F1 calves during early life.</p><p><strong>Methods: </strong>Twenty-three male Japanese Black-Holstein F1 calves were raised as a single cohort under identical feeding conditions from one to nine weeks old. All calves were fed the same milk replacer until weaning and had ad libitum access to starter and hay throughout the trial. Based on ruminal total methanogen abundance measured by quantitative real-time polymerase chain reaction at the end of the experiment, calves in the upper and lower quartiles were retrospectively classified as high- and low-methanogen groups, respectively (n = 6 per group). Rumen microbiota was characterized using 16S rRNA gene amplicon sequencing. Growth performance, feed intake, rumen fermentation characteristics, blood biochemical parameters, and rumen microbial profiles were compared between groups, and correlations with total methanogen abundance were assessed using all calves.</p><p><strong>Results: </strong>Total methanogen abundance was higher in the high-methanogen group compared to the low-methanogen group (6.56 vs. 5.53 log copy number/ng DNA; p<0.001). Average daily gain did not differ between groups (0.64 vs. 0.69 kg/d). Starter intake was higher in the high-methanogen group at 6 weeks (p<0.05) and was positively correlated with total methanogen abundance (r = 0.42; p<0.05). Molar percentage of ruminal propionate was higher in the low-methanogen group (28.59% vs. 20.58%; p<0.05). Methanobrevibacter was enriched in the high-methanogen group (p<0.05), whereas Prevotella (p<0.01), Succinivibrio (p<0.05), and Selenomonas (p<0.05) were enriched in the low-methanogen group.</p><p><strong>Conclusion: </strong>These findings suggest that ruminal total methanogen abundance during early life is related with distinct rumen fermentation patterns and microbial signatures, highlighting potential microbial and nutritional factors linked to methanogen abundance in calves.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"260205"},"PeriodicalIF":3.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterization of Hanwoo oviduct epithelial cell line for heat stress study.","authors":"Ismail Shaleh, Sung Woo Kim, Tae Sub Park, Joonghoon Park","doi":"10.5713/ab.250962","DOIUrl":"https://doi.org/10.5713/ab.250962","url":null,"abstract":"<p><strong>Objective: </strong>Heat-induced stress (HS) amid global warming compromise cattle reproductive performance causing economic loss including the vulnerable Korean native beef cattle, Hanwoo. Nevertheless, knowledge of response and effect in cattle oviduct epithelial cells (OEC) under HS remains lacking due to restricted commercial cell line and multi-omics data available. This research attempts to develop immortalized OEC derived from Hanwoo cattle as HS cell culture model and to investigate its HS-specific response by utilizing biomarkers comprising transcriptomic and proteomic analysis.</p><p><strong>Methods: </strong>Primary OEC was isolated from fresh Hanwoo oviduct and immortalized by using piggyBac transposon-mediated SV40T expression system. HS optimization was performed by detection of reactive oxygen species (ROS) and oxidative-endoplasmic reticulum (ER) stress biomarkers under varied HS and recovery time (4, 12, and 24 h). Optimized period was applied to generate differentially expressed genes (DEG) and protein (DEP) analyzed using gene ontology (GO) and pathway enrichment.</p><p><strong>Results: </strong>Immortalized OEC was successfully developed with high CDH1 positivity (98.2%) sorting. Increased intracellular ROS was detected at 4 h HS followed by rapid substantial upregulation of HSP70 and BiP protein and delayed upregulation of oxidative stress genes (SOD1, CAT, GPX1) peaked at 24 h HS period. In total, 510 DEG (fold change ≥|2|) and 258 DEP (fold change ≥|1.5|) were significantly altered under 24 h HS condition. Furthermore, GO and pathway enrichment analysis of the DEG-DEP list revealed upregulation processes related to oxidative-thermal stress and protein folding. Other altered processes involve immune response, cellular senescence, response to starvation, and extracellular matrix structures as enriched terms suggesting possible hindering effects to OEC reproductive role.</p><p><strong>Conclusion: </strong>The immortalized OEC provides a stable system to evaluate HS response in cattle oviducts. Transcriptomic and proteomic data from this study offer valuable resources for targeted molecular approach to attenuate detrimental HS effect in cattle reproduction.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}