Animal Bioscience最新文献

{"title":"Substituting effects of winged bean tuber-modified starches for cassava chip in concentrate diets on rumen fermentation, nutrient utilization, and blood metabolites in Thai native beef cattle.","authors":"Narirat Unnawong, Chaichana Suriyapha, Sompong Chankaew, Teppratan Rakvong, Anusorn Cherdthong","doi":"10.5713/ab.23.0516","DOIUrl":"10.5713/ab.23.0516","url":null,"abstract":"<p><strong>Objective: </strong>This study examined the effects of substituting winged bean tuber steam (WBTS) modified starches for cassava chips (CSC) in the concentrate diet on rumen fermentation, nutrient utilization, and blood metabolites in Thai-native beef cattle.</p><p><strong>Methods: </strong>Four Thai-native bulls were assigned randomly as a 4×4 Latin square design, which represents the amount of CSC replaced with WBTS in the concentrate mixture diets at 0%, 10%, 20%, and 30%.</p><p><strong>Results: </strong>Increasing levels of WBTS replacement for CSC in the concentrate diets had a quadratic effect on total dry matter (DM) intake (p<0.05). Replacement of WBTS at 20% and 30% for CSC did not alter total DM intake compared to 0% WBTS, whereas 10% WBTS replacement could significantly increase total DM intake by 0.41 kg DM/d compared to the control group. In addition, neutral detergent fiber (NDF) digestibility showed a quadratic increase (p<0.05) when CSC was substituted at various levels of WBTS in the concentrate diet (p<0.05). Replacement of CSC with WBTS at 10% and 20% showed higher NDF digestibility when compared to 0% replacement. There was a quadratic increase in blood glucose at 4 h post-feeding, and the average blood glucose value was significantly lower (p<0.01) when substituting CSC with WBTS. Substituting WBTS for CSC at 10% in the concentrate diet showed the highest blood glucose concentration when compared to other treatments. Replacing CSC with WBTS at 10% and 20% shows a higher concentration of C3 than those of other treatments (0% or 30%). The nitrogen (N) intake increased linearly (p<0.05) when substituting WBTS for CSC at all levels in the diet. Additionally, N retention and the ratio of N retention to N intake increased (p<0.05) when substituting WBTS for CSC at 10%, 20%, and 30% compared to 0%. The gross energy intake (GEI), digestible energy intake (DEI), and energy efficiency (DEI/GEI) were quadratically increased when substituted with various levels of WBTS for CSC in the concentrate diet.</p><p><strong>Conclusion: </strong>The findings indicate that substituting 10% of CSC in the concentrate diet with WBTS may be sufficient as an alternative feed resource for improving nutrient utilization and metabolic efficiency in beef cattle diets.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1726-1737"},"PeriodicalIF":2.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140955645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Runs of homozygosity analysis for selection signatures in the Yellow Korean native chicken.","authors":"Jaewon Kim, John Kariuki Macharia, Minjun Kim, Jung Min Heo, Myunghwan Yu, Hyo Jun Choo, Jun Heon Lee","doi":"10.5713/ab.24.0092","DOIUrl":"10.5713/ab.24.0092","url":null,"abstract":"<p><strong>Objective: </strong>Yellow Korean native chicken (KNC-Y) is one of the five pure Korean indigenous chicken breeds that were restored through a government project in 1992. KNC-Y is recognized for its superior egg production performance compared to other KNC lines. In this study, we performed runs of homozygosity (ROH) analysis to discover selection signatures associated with egg production traits in the KNC-Y population.</p><p><strong>Methods: </strong>A total of 675 DNA samples from KNC-Y were genotyped to generate single nucleotide polymorphism (SNP) data using custom 60K Affymetrix SNP chips. ROH analysis was performed using PLINK software, with predefined parameters set for the analysis. The threshold of ROH island was defined as the top 1% frequency of SNPs withing the ROH among the population.</p><p><strong>Results: </strong>In the KNC-Y population, a total of 29,958 runs of homozygosity (ROH) fragments were identified. The average total length of ROH was 120.84 Mb, with each ROH fragment having an average length of 2.71 Mb. The calculated ROH-based inbreeding coefficient (FROH) was 0.13. Furthermore, we revealed the presence of ROH islands on chromosomes 1, 2, 4, 5, 7, 8, and 11. Within the identified regions, a total of 111 genes were annotated, and among them were genes related to economic traits, including PRMT3, ANO5, HDAC4, LSS, PLA2G4A, and PTGS2. Most of the overlapping quantitative trait locus regions with ROH islands were found to be associated with production traits.</p><p><strong>Conclusion: </strong>This study conducted a comprehensive analysis of ROH in the KNC-Y population. Notably, among the findings, the PTGS2 gene is believed to play a crucial role in influencing the laying performance of KNC-Y.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1683-1691"},"PeriodicalIF":2.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140955702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of replacing concentrates with cassava root-top silage on feed utilization, rumen fermentation, blood parameters and growth performance in beef cattle.","authors":"Nirawan Gunun, Randorn Phimda, Nonthasak Piamphon, Walailuck Kaewwongsa, Darunee Puangbut, Chatchai Kaewpila, Waroon Khota, Anusorn Cherdthong, Pongsatorn Gunun","doi":"10.5713/ab.24.0076","DOIUrl":"10.5713/ab.24.0076","url":null,"abstract":"<p><strong>Objective: </strong>This experiment aimed to evaluate the effects of replacing concentrates with cassava root-top silage (CARTOS) on feed intake, digestibility, rumen fermentation, blood parameters, and growth performance of beef cattle.</p><p><strong>Methods: </strong>Twenty crossbred bulls with a body weight (BW) of 226±56 kg were randomly assigned to one of five treatments for 90 d in a randomized complete block design having four blocks based on BW. The concentrates were replaced by CARTOS at levels of 0%, 25%, 50%, 75%, and 100% dry matter (DM). Animals were fed dietary treatments at 1.8% BW, with rice straw offered ad libitum.</p><p><strong>Results: </strong>The DM and crude protein (CP) intake were decreased (p<0.01, p = 0.04) when the diet's CARTOS level was increased. The digestibility of DM, OM, and CP were not different among treatments, while fiber digestibility was increased with the inclusion of CARTOS (p = 0.03). The addition of CARTOS to replace concentrates did not change ruminal pH or volatile fatty acid proportions except for acetic acid, which increased with the addition of CARTOS (p = 0.03). The ruminal ammonia-nitrogen (NH3-N) was decreased (p<0.01) with increasing levels of CARTOS. The blood glucose and blood urea nitrogen decreased (p = 0.01) with the addition of CARTOS at 100%, whereas total protein and hematological parameters did not change with increasing levels of CARTOS. The use of CARTOS to substitute concentrates at 75% and 100% decreased average daily gain (ADG) and gain to feed ratio (G:F) (p<0.01); therefore, the addition of CARTOS up to 50% maintained ADG and G:F in beef cattle.</p><p><strong>Conclusion: </strong>CARTOS can replace concentrates up to 50% in beef cattle diets without adversely affecting feed intake, nutrient digestibility, rumen fermentation characteristics, blood parameters, or growth performance of beef cattle.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1751-1758"},"PeriodicalIF":2.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the global mechanism related to carnosine synthesis in the pectoralis major of slow-growing Korat chickens using a proteomic approach.","authors":"Panpradub Sinpru, Chanadda Suwanvichanee, Rujjira Bunnom, Satoshi Kubota, Jirawat Yongsawatdigul, Wittawat Molee, Kanjana Thumanu, Amonrat Molee","doi":"10.5713/ab.24.0119","DOIUrl":"10.5713/ab.24.0119","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to find global mechanisms related to carnosine synthesis in slow-growing Korat chickens (KRC) using a proteomic approach.</p><p><strong>Methods: </strong>M. pectoralis major samples were collected from 10-week-old female KRC including low-carnosine (LC, 2,756.6±82.88 μg/g; n = 5) and high-carnosine (HC, 4,212.5 ±82.88 μg/g; n = 5).</p><p><strong>Results: </strong>We identified 152 common proteins, and 8 of these proteins showed differential expression between the LC and HC groups (p<0.05). Heat shock 70 kDa protein 8, Heat shock 70 kDa protein 2, protein disulfide isomerase family A, member 6, and endoplasmic reticulum resident protein 29 were significantly involved in protein processing in the endoplasmic reticulum pathway (false discovery rate<0.05), suggesting that the pathway is related to differential carnosine concentration in the M. pectoralis major of KRC. A high concentration of carnosine in the meat is mainly involved in low abundances of Titin isoform Ch12 and Connectin and high abundances of M-protein to maintain homeostasis during muscle contraction. These consequences improve meat characteristics, which were confirmed by the principal component analysis.</p><p><strong>Conclusion: </strong>Carnosine synthesis may occur when muscle cells need to recover homeostasis after being interfered with carnosine synthesis precursors, leading to improved muscle function. To the best of our knowledge, this is the first study to describe in detail the global molecular mechanisms in divergent carnosine contents in meat based on the proteomic approach.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1692-1701"},"PeriodicalIF":2.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141974916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of cholesterol-loaded cyclodextrin treatment on boar sperm cryopreservation.","authors":"Silong Zhang, Hanbing Zhang, Kexiong Liu, Xiaoling Xu, Yusheng Qin, Linli Xiao, Chunmei Zhou, Jianliang Wu, Yan Liu, Jiahua Bai","doi":"10.5713/ab.24.0030","DOIUrl":"10.5713/ab.24.0030","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the efficacy of different concentrations of cholesterolloaded cyclodextrin (CLC) on cryopreservation in boar sperm quality.</p><p><strong>Methods: </strong>In this study, we treated boar sperm with different concentrations of CLC before freezing and analyzed the sperm cholesterol concentration, plasma membrane, acrosome integrity rate and total motility rate before and after freeze-thawing. We also investigated the levels of reactive oxygen species (ROS), malondialdehyde (MDA), adenosine triphosphate (ATP), and structural- and oxidative-damage related proteins in all groups after thawing.</p><p><strong>Results: </strong>The results revealed that the cholesterol concentration of the CLC-treated groups was higher than that of the control group, both before freezing and after thawing (p<0.05). The plasma membrane integrity rate, acrosome integrity rate, and total motility rate of sperm were also enhanced after thawing in the CLC-treated group (all p<0.05). Moreover, ROS and MDA production and ATP loss were reduced in CLC-treated sperm during freezing and thawing (p<0.05). Finally, CLC pretreatment partially prevented the consumption of various proteins involved in metabolism including capping actin protein of muscle Z-line subunit beta (CAPZB), heat shock protein 90 alpha family class A member 1 (HSP90AA1) and phosphoglycerate mutase 2 (PGAM2) (p<0.05).</p><p><strong>Conclusion: </strong>The CLC treatment increased cholesterol concentration and decreased structural injury and oxidative damage during boar sperm freezing and thawing, improving the efficacy of sperm cryopreservation in boar.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1558-1567"},"PeriodicalIF":2.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140955635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary deoxynivalenol on growth performance and organ accumulation of growing pigs.","authors":"Ah Reum Son, Seung Youp Shin, Yoon Soo Song, Bokyung Hong, Beob Gyun Kim","doi":"10.5713/ab.24.0177","DOIUrl":"10.5713/ab.24.0177","url":null,"abstract":"<p><strong>Objective: </strong>The present study aimed to study effects of a chronic feeding of deoxynivalenol (DON) on growth performance, organ weight, organ DON accumulation, and blood parameters in pigs.</p><p><strong>Methods: </strong>Forty-eight castrated male pigs with a body weight of 10.4 kg (standard deviation = 1.7) were assigned to one of 2 diet groups in a randomized complete block design with 6 blocks of pens per diet and 4 pigs per pen. A corn-soybean meal-based control diet was prepared to contain a low DON concentration of 0.28 mg/kg. Corn distillers dried grains with solubles naturally contaminated with DON were used at 30.0% to prepare a contaminated diet with a high DON concentration of 1.8 mg/kg. During the 56-day experimental period, body weight and feed intake were recorded every 14 days. A pig from each pen was euthanized for the collection of organs and muscle every 14 days.</p><p><strong>Results: </strong>Gain:feed in pigs fed the contaminated diet during days 14 to 28 and days 28 to 42 were less (p<0.05) compared with the control group. As increasing feeding period, the DON concentrations in fresh liver increased during days 14 to 28 and then decreased during the subsequent periods in the DON group, whereas the DON concentrations in fresh liver were constant during the experimental period in the control group (quadratic interaction p = 0.049). The DON concentration in the kidneys in the DON group was greater (p = 0.002) than that in the control group regardless of feeding period. On day 56, the granulocyte count in the DON group was less (p = 0.035) than the control group.</p><p><strong>Conclusion: </strong>A chronic feeding of DON for 14 to 42 days decreased gain:feed in pigs, and dietary DON naturally contaminated in corn distillers dried grains with solubles accumulated in the liver during days 14 to 28.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1614-1621"},"PeriodicalIF":2.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Freezing Hu ram semen: influence of different penetrating cryoprotectants and egg yolk level on the post-thaw quality of sperm.","authors":"Liuming Zhang, Caiyu Jiang, Xuyang Wang, Tariq Sohail, Yuxuan Sun, Xiaomei Sun, Jian Wang, Yongjun Li","doi":"10.5713/ab.24.0167","DOIUrl":"10.5713/ab.24.0167","url":null,"abstract":"<p><strong>Objective: </strong>The Hu sheep is a renowned breed known for its high reproductive rate. It is in estrus all year round, and its breeding population is gradually expanding. However, the current techniques for cryopreserving semen have limited effectiveness, which hinders the continuous development of this species. The purpose of this study is to explore the effects of different penetrating cryoprotectants (CPAs) and egg yolk (EY) concentrations on the cryopreservation of Hu ram semen to determine the most effective combination.</p><p><strong>Methods: </strong>In this study, the effects of glycerol (GLY), ethylene glycol (EG), dimethylacetamide, dimethyl sulfoxide, different proportions of GLY and EG, EY on sperm quality after thawing were investigated by detecting sperm total motility (TM), progressive motility (PM), straight-line velocity, curvilinear velocity, average path velocity, amplitude of lateral head displacement, wobble movement coefficient, average motion degree, functional integrity (plasma membrane integrity, acrosome integrity) and reactive oxygen species (ROS) level.</p><p><strong>Results: </strong>When GLY and EG were added together, compared to other concentration groups, 6% GLY significantly (p<0.05) increased TM, PM, plasma membrane integrity, and acrosome integrity of thawed sperm. Additionally, it significantly (p<0.05) decreased the ROS level of sperm. In this study, the TM, PM, and membrane integrity of the 6% EG were significantly (p<0.05) higher than those of the control, 1% GLY+5% EG and 6% GLY+6% EG groups. Compared to other concentration groups, 20% EY significantly (p<0.05) improved the TM, PM, and plasma membrane integrity of thawed sperm. However, the integrity of the acrosome increased with the higher concentration of EY.</p><p><strong>Conclusion: </strong>In conclusion, the post-thawed Hu ram semen diluted with a diluent containing 6% GLY and 20% EY exhibited higher quality compared to the other groups.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1548-1557"},"PeriodicalIF":2.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366523/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Encyclopedia of Animal Nutrition (2nd Edition).","authors":"Yoo Yong Kim","doi":"10.5713/ab.24.0001B","DOIUrl":"10.5713/ab.24.0001B","url":null,"abstract":"","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":"37 9","pages":"1660"},"PeriodicalIF":2.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and characterization of a novel microbial GH9 glucanase, IDSGLUC9-4, isolated from sheep rumen.","authors":"Yongzhen Zhu, Shuning Bai, Nuo Li, Jun-Hong Wang, Jia-Kun Wang, Qian Wang, Kaiying Wang, Tietao Zhang","doi":"10.5713/ab.24.0138","DOIUrl":"10.5713/ab.24.0138","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to identify and characterize a novel endo-β-glucanase, IDSGLUC9-4, from the rumen metatranscriptome of Hu sheep.</p><p><strong>Methods: </strong>A novel endo-β-glucanase, IDSGLUC9-4, was heterologously expressed in Escherichia coli and biochemically characterized. The optimal temperature and pH of recombinant IDSGLUC9-4 were determined. Subsequently, substrate specificity of the enzyme was assessed using mixed-linked glucans including barley β-glucan and Icelandic moss lichenan. Thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), matrix assisted laser desorption ionization time of flight mass spectrometry analyses were conducted to determine the products released from polysaccharides and cello-oligosaccharides substrates.</p><p><strong>Results: </strong>The recombinant IDSGLUC9-4 exhibited temperature and pH optima of 40°C and pH 6.0, respectively. It exclusively hydrolyzed mixed-linked glucans, with significant activity observed for barley β-glucan (109.59±3.61 μmol/mg min) and Icelandic moss lichenan (35.35±1.55 μmol/mg min). TLC and HPLC analyses revealed that IDSGLUC9-4 primarily released cellobiose, cellotriose, and cellotetraose from polysaccharide substrates. Furthermore, after 48 h of reaction, IDSGLUC9-4 removed most of the glucose, indicating transglycosylation activity alongside its endo-glucanase activity.</p><p><strong>Conclusion: </strong>The recombinant IDSGLUC9-4 was a relatively acid-resistant, mesophilic endo-glucanase (EC 3.2.1.4) that hydrolyzed glucan-like substrates, generating predominantly G3 and G4 oligosaccharides, and which appeared to have glycosylation activity. These findings provided insights into the substrate specificity and product profiles of rumen-derived GH9 glucanases and contributed to the expanding knowledge of cellulolytic enzymes and novel herbivore rumen enzymes in general.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1581-1594"},"PeriodicalIF":2.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skin proteomic screening and functional analysis of differential proteins associated with coat color in sheep (Ovis aries).","authors":"Zhihong Yin, Shitong Hao, Yuanyuan Zhao, Jinglong Li, Yunli Cui, Yaming Ge, Quanhai Pang","doi":"10.5713/ab.24.0014","DOIUrl":"10.5713/ab.24.0014","url":null,"abstract":"<p><strong>Objective: </strong>Coat color is an important characteristic and economic trait in domestic sheep. In this study, we explored the potential mechanisms and the signaling pathways involved in coat color regulation for sheep.</p><p><strong>Methods: </strong>Isobaric tags for relative and absolute quantification (iTRAQ) technology was used to catalog global protein expression profiles in skin of sheep with black versus white coat color. Immunofluorescence was used to observe the expression localization of differential protein. Western blot and quantitative real time polymerase chain reaction (qRT-PCR) were used to evaluate their role in the coat color formation of sheep.</p><p><strong>Results: </strong>A total of 136 differential proteins were obtained in different coat colors, including 101 up-regulated and 35 down-regulated. Pigmentation function entries were enriched through gene ontology annotation. Tyrosine metabolism and platelet activation signaling pathway were extracted by Kyoto encyclopedia of genes and genomes analysis. Apolipoprotein A-1 (APOA1) and fibrinogen alpha chain (FGA) were found to be critical differential proteins by the interaction of differential proteins in the direct-interaction network diagram. Strikingly, twenty candidate differential proteins were screened, from which beta-actin (ACTB) protein showed higher expression in white sheep skin, while albumin (ALB), APOA1, MAOA (amine oxidase) and FGA proteins showed higher expression in black sheep skin, which was validated by immunofluorescence, western blot, and qRT-PCR.</p><p><strong>Conclusion: </strong>This study identified several novel proteins that may be involved in the coat color formation of sheep. The white and black sheep skin proteome profiles obtained provide a valuable resource for future research to understand the network of protein expression controlling skin physiology and melanogenesis in sheep.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1503-1516"},"PeriodicalIF":2.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140955742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}