Animal Bioscience最新文献

{"title":"Liquid chromatograph-mass spectrometry metabolomics uncovers potential biomarkers of semen cryo-injury in goats.","authors":"Shun Wu, Guolin Chen, Siyuan Zhan, Linjie Wang, Jiaxue Cao, Jiazhong Guo, Li Li, Hongping Zhang, Lili Niu, Tao Zhong","doi":"10.5713/ab.24.0435","DOIUrl":"10.5713/ab.24.0435","url":null,"abstract":"<p><strong>Objective: </strong>Semen cryopreservation acts a crucial role in enhancing breed improvement and conserving genetic resources. However, it often leads to decreased sperm activity and reduced pregnancy rates. Despite significant advancements in semen freezing techniques for goats, the precise factors and mechanisms causing cryo-injury remain unclear.</p><p><strong>Methods: </strong>In this study, we examined the motility characteristics of fresh semen versus frozen-thawed semen and investigated changes in the metabolite profiles of seminal plasma using liquid chromatograph-mass spectrometry.</p><p><strong>Results: </strong>A total of 364 differentially expressed metabolites (DEMs) were identified between fresh and frozen-thawed semen samples. Among these, 185 metabolites were significantly up-regulated, while 179 were down-regulated (p<0.05). The majority of these DEMs belonged to lipids and lipid-like molecules, as well as organic acids and derivatives. The Kyoto encyclopedia of genes and genomes indicated that these DEMs were primarily involved in pathways related to amino acid synthesis and metabolism. Additionally, metabolite set enrichment analysis underscored the critical role of amino acid synthesis and metabolic pathways in semen cryopreservation. Specific metabolites such as alanine, proline, phenylalanine, tryptophan, tyrosine, adenosine, citric acid, flavin adenine dinucleotide, and choline emerged as potential biomarkers for sperm cryo-injury in goats.</p><p><strong>Conclusion: </strong>These findings provide valuable insights into enhancing the quality of semen cryopreservation in goats, contributing to improved breeding and genetic resource conservation efforts.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"629-640"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the Purity and Intrinsic Properties of Ovine Dermal Papilla Cells through Flow Cytometry Sorting and Cellular Interactions.","authors":"Yuan Gou, Tingyan Hu, Lijuan Zhu, Xiaoyang Lv, Yutao Li, Rui Su, Zhenghai Song, Shanhe Wang, Wei Sun","doi":"10.5713/ab.24.0805","DOIUrl":"https://doi.org/10.5713/ab.24.0805","url":null,"abstract":"<p><strong>Objective: </strong>Dermal Papilla Cells (DPCs) play a crucial role in regulating hair follicle development and serve as a valuable in vitro model for screening and analyzing the genes associated with this process. However, current methods for isolating ovine DPCs primarily rely on mechanical techniques, which present several limitations. The aim of this study is to establish a method for isolating and culturing ovine DPCs with high purity and retains their intrinsic properties.</p><p><strong>Methods: </strong>We identified sheep DPC membrane-specific genes using single-cell transcriptomic data, validated by immunostaining and flow cytometry. Antibody-labelled DPCs were isolated, cultured, and assessed via FACS, comparing their purity with conventional mechanical isolation. Mechanically isolated and flow-sorted DPCs were analyzed through agglutination, CCK-8, and EDU staining. Furthermore, we examined the biological properties of isolated DPCs in conditioned media using CCK-8, EDU, and qRT-PCR assays.</p><p><strong>Results: </strong>PDGFRA was identified as a marker for ovine DPCs. Flow cytometry showed that PDGFRA-labeled DPCs made up 1.54% of the hair follicle cell population, with 1.92% live DPCs obtained via FACS. The isolated DPCs demonstrated agglutination and were positive for ALP, Versican, and α-SMA. Antibody labeling yielded higher DPC purity compared to mechanical isolation, highlighting its efficiency.Accordingly, the addition of conditioned media from mechanically isolated DPCs significantly enhanced agglutination, cell viability, proliferation, and inductive capacity of the sorted DPCs. However, as the number of passages increased, the sorted DPCs demonstrated significant disadvantages in cell agglutination, proliferation rate, and viability compared to mechanically isolated DPCs. Accordingly, the addition of conditioned media from mechanically isolated DPCs significantly enhanced agglutination, cell viability, proliferation, and inductive capacity of the sorted DPCs.</p><p><strong>Conclusion: </strong>This study highlights the effectiveness of antibody labeling and flow cytometry for isolating functionally pure DPCs, as well as the potential of conditioned media to maintain the functional properties of these cells.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary energy levels on productivity, fat deposition, and biochemical parameters of Woorimatdag1 breeder pullets.","authors":"Hyojun Choo, Chunik Lim, Hyeonkwon Kim, Kangnyeong Heo, Euichul Hong","doi":"10.5713/ab.24.0369","DOIUrl":"10.5713/ab.24.0369","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate effects of apparent metabolizable energy (AMEn) levels in diets on productivity, fat deposition, and biochemical parameters of Woorimatdag1 (WMD1) breeder pullets.</p><p><strong>Methods: </strong>A total of 240 four-week-old WMD1 breeder pullets were divided into four dietary groups with five replicates (12 birds per replicate). These groups had the following dietary energy levels: standard metabolizable energy (SME), SME-200, SME-100, and SME+100 (diets containing 2,800, 2,600, 2,700, and 2,900 kcal AMEn/kg, respectively). These pullets were provided with diets and water ad libitum until 16 weeks old.</p><p><strong>Results: </strong>Weight gain was significantly (p<0.05) higher in SME-100, SME, and SME+100 groups than in the SME-200 group. SME+100 and SME groups exhibited significantly (p<0.05) improved feed conversion ratio compared to the SME-200 group. Laying ages of 30% egg production occurred significantly (p<0.05) earlier in SME-100, SME, and SME+100 groups than in the SME-200 group. SME and SME+100 groups had significantly (p<0.05) higher liver fat (%) than the SME-200 group. Additionally, the SME+100 group had higher (p<0.05) abdominal fat (%) than other groups. However, blood parameters were not significantly different among dietary groups.</p><p><strong>Conclusion: </strong>SME-100 (2,700 kcal AMEn/kg) might be suitable for improving productivity and fat deposition of WMD1 breeder pullets.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"707-716"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of umami peptides and mechanism of the interaction with umami receptors T1R1/T1R3 in pigeon meat.","authors":"Yue Zheng, Mengnan Cao, Dengyong Liu","doi":"10.5713/ab.24.0425","DOIUrl":"10.5713/ab.24.0425","url":null,"abstract":"<p><strong>Objective: </strong>Pigeon meat offer an ideal source for extracting fresh flavor peptides. These peptides not only enhance the taste of food but also have potential health benefits, including providing low-sugar, low-sodium, and low-calorie options for individuals with conditions like diabetes, hypertension, and obesity. Therefore, further research into the pigeon industry holds promise for addressing both economic and nutritional needs.</p><p><strong>Methods: </strong>To explore umami peptides and their molecular binding mechanisms with umami receptor type 1 member 1 in pigeon meat, an enzymatic hydrolysate product is isolated, analyzed, and subjected to sensory evaluation. Fifteen peptides with high freshness characteristics are separated and identified by ultrafiltration, gel separation, reverse performance liquid chromatography, and nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS).</p><p><strong>Results: </strong>The molecular docking results show that the amino acid residue Glu128 is a common ligand binding site for all of the fresh-flavored peptides to taste T1R1/T1R3 receptors and it exerts freshness-presenting effects with 15 fresh-flavored peptides through hydrogen bonding, electrostatic interactions, salt bridges, and hydrophobic interactions.</p><p><strong>Conclusion: </strong>This study provides a theoretical basis and technical support for the subsequent development of flavor peptide products in pigeon meat.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"739-751"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of supplementing rumen-protected rubber seed oil to dairy cattle on feed digestibility and milk production.","authors":"Pongsatorn Gunun, Norakamol Laorodphan, Warachit Phayom, Walailuck Kaewwongsa, Chatchai Kaewpila, Waroon Khota, Nirawan Gunun","doi":"10.5713/ab.24.0287","DOIUrl":"10.5713/ab.24.0287","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to determine the effect of rumen-protected rubber seed oil (RPRSO) supplementation on feed digestibility, milk yield, and milk composition in tropical dairy cows.</p><p><strong>Methods: </strong>Twelve crossbred Holstien-Friesian dairy cows (75% Holstein-Friesian, 25% Thai native breed) with a mean body weight of 460±30 kg and 20±5 days in milk were randomly assigned to 1 of 3 treatments according to a completely randomized design. The treatments were as follows: a basal diet without rumen-protected fat (RPF) (control) or supplementation of rumen-protected palm oil (RPPO) at 300 g/h/d and RPRSO at 300 g/h/d. Each cow was fed a total mixed ration ad libitum.</p><p><strong>Results: </strong>The nutrient intake was similar among treatments (p>0.05). Adding RPF did not affect nutrient digestibility, while organic matter (OM) digestibility increased in dairy cows receiving RPRSO (p<0.01). Blood urea nitrogen (BUN), total protein, or glucose did not alter among treatments (p>0.05), while triglycerides and cholesterol were increased when cows were fed RPPO (p<0.01). Adding RPF increases milk yield in cows (p<0.01). The supplementation of RPRSO increased milk fat (p = 0.04). Milk fat yield was higher in RPPO and highest in RPRSO (p<0.01). The addition of RPF increased the oleic acid (OA, C18:1 cis-9) in milk (p = 0.01). In addition, cows fed RPRSO increased linoleic acid (LA; C18:2ω6 cis-9,12+trans-9,12) and α-linolenic acid (ALA; C18-3ω3 cis-9,12,15) in milk (p<0.01). The addition of RPF increased milk unsaturated fatty acids and monounsaturated fatty acids (p≤0.04). The polyunsaturated fatty acid (PUFA) in milk increased with RPRSO supplementation (p<0.01).</p><p><strong>Conclusion: </strong>Supplementation of RPRSO during early lactation can increase feed digestibility and the concentration of milk fat with PUFA (LA and ALA) in tropical dairy cows.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"665-672"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"- Invited Review - Challenges and constraints to the sustainability of poultry farming in Republic of Korea.","authors":"Sidong Kim","doi":"10.5713/ab.24.0641","DOIUrl":"10.5713/ab.24.0641","url":null,"abstract":"<p><p>As of 2022, the Republic of Korea accounted for 0.8% of global chicken meat production and 0.9% of global egg production. The country achieved self-sufficiency rates of 83.1% for chicken meat and 99.4% for eggs, demonstrating significant quantitative and qualitative growth to meet domestic demand. Although the industry is trending towards expansion and specialization, it faces several challenges in achieving sustainable poultry production. Key challenges in Korea include highly pathogenic avian influenza and pest issues, climate change and the push for carbon neutrality, reliance on imported breeding stock, insufficient preparedness for expanding cage space per laying hen, post-settlement payment systems for egg sales and an oversupply of chicken meat, and the aging poultry farming population and the closure of farms unable to secure successors. Following strategies are proposed to overcome or mitigate challenges mentioned above: (1) enhancing farm biosecurity and implementing vaccination policies for disease control, (2) modernizing facilities and promoting carbon-neutral practices to adapt to climate change, (3) diversifying breeding stocks across multiple locations and developing domestic strains, (4) implementing policies and supporting farms based on a comprehensive readiness assessment of all farms regarding expanded cage space requirements, (5) improving market transparency for the egg industry and regulating supply and demand in the broiler industry, and (6) offering incentives for farm succession, attracting labor, and promoting coexistence between corporations, rural communities, and small farms. In conclusion, the sustainable development of Korea's poultry industry is not a simple task. It requires a comprehensive approach considering economic efficiency, animal welfare, environmental protection, food security, and the symbiosis with rural communities. This approach necessitates efficient cooperation among all stakeholders, including the government, farmers, integrators, retailers, and research institutions, along with a comprehensive, phased strategy for both short- and long-term goals.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"829-844"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"- Invited Review - Challenges and constraints to the sustainability of poultry farming in China.","authors":"Fei Zhang, Zhuting Chen, Jiayi Shi, Chenglong Han, Qinyi Zhan, Zhouzheng Ren, Xiaojun Yang","doi":"10.5713/ab.24.0794","DOIUrl":"10.5713/ab.24.0794","url":null,"abstract":"<p><p>China's poultry industry is characterized by large-scale production and rich breeds, presenting both opportunities and challenges. In 2023, the industry produced 10.79 billion broilers, 28.38 million tons of eggs, and 4.88 billion waterfowl. The foundation of a thriving poultry industry lies in the continuous improvement of breeds. For instance, new lines of Lueyang black-boned chickens have been developed using genomic selection breeding, with a focus on improving production performance and unlocking their high-quality genetic potential. Precision nutrition programs enhance the expression of poultry's genetic potential and improve feed utilization efficiency. The five-dimensional feed evaluation system and the comprehensive National Feed Database provide formulators with accurate nutritional parameters of feed. Additionally, the concept of \"nutrition power\" and the \"five-ring gold standard\" enable researchers to analyze poultry's digestive physiology more effectively. Feeding management plays a crucial role in optimizing genetic potential and the effectiveness of precision nutrition. To further boost production efficiency, smart farming systems have been implemented, incorporating intelligent management of environmental factors, animal parameters, and poultry health tracking. Meanwhile, in order to improve material utilization efficiency across the entire poultry production chain and support the sustainable development of the poultry industry, it is essential to optimize and promote the application of the Poultry-Crop interaction systems. In summary, strengthening fundamental research in poultry, optimizing smart poultry farm platforms, and implementing Poultry-Crop Interacting systems will drive the sustainable development of China's poultry industry.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"789-801"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of fig and pineapple powder on metabolite compounds and health-promoting properties in fermented goat meat sausage.","authors":"Shine Htet Aung, Rupasinghe Ramesh Nimantha, Young-Sun Choi, Aera Jang, Jun Heon Lee, Ki-Chang Nam","doi":"10.5713/ab.24.0526","DOIUrl":"10.5713/ab.24.0526","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this research was to identify the metabolite compounds and health-promoting properties of goat meat fermented sausages containing different amounts of fig and pineapple powder (0.1%, 0.25%, and 0.5%), which are presented by F1, F2, F3 and P1, P2, P3, separately.</p><p><strong>Methods: </strong>Fermented sausages were manufactured from the lean meat of female goats. The samples extracted from the goat meat fermented sausages were evaluated for their metabolite compounds, antioxidants, and angiotensin-converting enzyme inhibitory activity.</p><p><strong>Results: </strong>The results showed that the diphenyl picrylhydrazyl radical scavenging activity, azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity, and angiotensin-converting enzyme inhibitory activity were the highest in F3 (p<0.05), whereas the hydroxyl radical scavenging capacity was the highest in P3 (p<0.05). In addition, F3 and F2 showed the highest superoxide anion radical scavenging activity (p<0.05), whereas F3 and P3 showed the highest copper chelating activity (p<0.05). Based on the metabolite compounds, hydrophobic amino acids, aromatic amino acids, and bitter amino acids were abundant in F3. Both F3 and P3 contained high concentrations of umami-producing compounds.</p><p><strong>Conclusion: </strong>The incorporation of 0.5% fig powder into goat meat fermented sausage showed improved biological activities based on metabolite compounds.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"752-764"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of host-specific and locally isolated multi-strain probiotics on piglet performance, mortality, inflammatory response, and gut microbiome.","authors":"Katatikarn Sahatsanon, Panneepa Sivapirunthep, Korawan Sringarm, Chaiwat Arjin, Patipan Hnokaew, Kamon Chaweewan, Chanporn Chaosap","doi":"10.5713/ab.24.0556","DOIUrl":"10.5713/ab.24.0556","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to assess the impact of host-specific and locally isolated multistrain probiotics on piglet performance, mortality, inflammatory responses, and gut microbiome.</p><p><strong>Methods: </strong>A total of 52 piglet litters-34 from Landrace sows and 18 from Large White sows-were allocated to two groups: a control group and a multi-strain probiotic group. The probiotic group comprised seven strains of lactic acid bacteria (MLAB): Lactobacillus brevis, Lactobacillus reuteri, Lactobacillus paraplantarum, Lactococcus lactis, Lactobacillus pentosus, Weissella cibaria, and Pediococcus pentosaceus. Each strain was included in equal concentrations, resulting in a final liquid mixture containing 109 colony forming units/mL. The MLAB group received the probiotics orally starting from 7 days of age until weaning at four weeks. Following weaning, supplementation continued via feed spraying for an additional four weeks.</p><p><strong>Results: </strong>MLAB supplementation did not significantly affect piglet performance but showed a trend towards reducing the mortality rate (p = 0.06). It influenced the inflammatory response by upregulating the expression of anti-inflammatory cytokines interleukin (IL)-4 and IL-10 (p<0.05). Microbial community analysis indicated that MLAB supplementation increased both microbial diversity (Simpson index: p = 0.06) and species richness (Chao1 index: p = 0.02). Piglets receiving MLAB had a significantly higher abundance of the phylum Firmicutes (p<0.01) compared to the control group, while the abundance of the phylum Bacteroidota was markedly reduced (p<0.01). In addition, the relative abundance of the bacterial genera Prevotellaceae_NK3B31 (p<0.01) and Chlamydia (p = 0.03) was lower in the MLAB group.</p><p><strong>Conclusion: </strong>Overall, these results suggest that while MLAB supplementation does not directly improve piglet growth performance, it has the potential to improve immune function and promote a healthier gut microbiota in weaning piglets, which could ultimately reduce mortality rates.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"717-727"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intermittent cold stimulation acclimates broilers to acute cold stress by affecting cardiac lipid metabolism.","authors":"Yong Zhang, Minghang Chang, Qiang Xue, Hongyu Wang, Yuanyuan Liu, Haidong Wei, Jianhong Li","doi":"10.5713/ab.24.0389","DOIUrl":"10.5713/ab.24.0389","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate whether intermittent cold stimulation can induce adaptation in broilers to acute cold stress (ACS) by regulating the lipid metabolism of hearts.</p><p><strong>Methods: </strong>CS0 were kept at normal rearing temperature, while CS3 and CS5 were exposed to 3°C for 3 and 5 hours, respectively, on alternate days lower than CS0 from 15d to 35d. On 50d, broilers in three groups were exposed to ACS at 10°C for 12 hours (Y12). The levels of corticosterone (CORT) and liothyronine (T3), mRNA and protein levels of heart adenosine monophosphate (AMP)-activated protein kinase/mammalian target of rapamycin (AMPK/mTOR) pathway genes were assessed at 36 d, 50 d and Y12.</p><p><strong>Results: </strong>At 36d, mRNA levels of AMPKα, acyl-CoA oxidase (ACO), mTOR, sterolregulatory element binding protein (SREBP), stearoyl-coA desaturase (SCD), acetyl-coA carboxylase (ACC), fatty acid synthase (FAS) and protein level of peroxisome proliferatorsactivated receptor α (PPARα) in CS3 and CS5 were significantly lower than those in CS0 (p<0.05). At 50d, compared to CS0, mRNA levels of PPARα, carnitine palmitoyltransferase1 (CPT1), ACO, tuberous sclerosis complex (TSC), SREBP and SCD, as well as protein levels of p-AMPKα/AMPKα, PPARα and SREBP were significantly increased in CS5 (p<0.05). At Y12, the levels of T3 in CS3 and CS5 were significantly higher than those in CS0 (p<0.05), mRNA levels of CPT1, ACO, SREBP, SCD and protein levels of p-AMPKα/AMPKα, SREBP, and FAS were significantly higher in CS5 than in CS0 and CS3 (p<0.05). However, compared to 50d, at Y12, mRNA levels of AMPKα, CPT1 and ACO in CS3 and CS5 significantly decreased (p<0.05), while protein levels of p-AMPKα/AMPKα significantly increased (p<0.05).</p><p><strong>Conclusion: </strong>This study suggested that intermittent cold stimulation at 3°C lower than normal rearing temperature for 5h could help broilers adapt to the ACS by promoting heart lipid metabolism.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"775-787"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}