Animal Bioscience最新文献

{"title":"Animal amino acid sensor - A review.","authors":"Yongding Ke, Xinyu Peng, Chengchuang Song, Xingtang Fang, Yanhong Wang, Chunlei Zhang","doi":"10.5713/ab.24.0366","DOIUrl":"10.5713/ab.24.0366","url":null,"abstract":"<p><p>Cell growth and metabolism necessitate the involvement of amino acids, which are sensed and integrated by the mammalian target of rapamycin complex 1 (mTORC1). However, the molecular mechanisms underlying amino acid sensing remain poorly understood. Research indicates that amino acids are detected by specific sensors, with the signals being relayed to mTORC1 indirectly. This paper reviews the structures and biological functions of the amino acid sensors identified thus far. Additionally, it evaluates the potential role these sensors play in the developmental changes of the livestock production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"198-208"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beta-glucans supplementation for sows during gestation and lactation.","authors":"Julia Delpupo Coelho, Pedro Henrique Watanabe, Tiago Silva Andrade, Rayssa Aline Rocha Santos, Marcelo Emersom Costa Santos, Ingrid Barbosa Mendonça, Deborah Marrocos Sampaio Vasconcelos, Isaac Neto Goes Silva, Manoeal Wanamark David Ferreira Filho, Leonardo Augusto Fonseca Pascoal, Ednardo Rodrigues Freitas","doi":"10.5713/ab.24.0516","DOIUrl":"https://doi.org/10.5713/ab.24.0516","url":null,"abstract":"<p><strong>Purpose: </strong>The aim of the present study was to evaluate the effects of dietary beta-glucans supplementation for gestation and lactation sows of 1st, 2nd or 3rd parity on sows and litters performance, milk composition and Brix value of colostrum, hemogram and serum immunoglobulin G concentration of sows and piglets.</p><p><strong>Methods: </strong>A total of 78 sows were distributed in a completely randomized design, in a 2x3 factorial scheme, with two supplementation levels (0 and 450 mg/kg of beta-glucans) and three parities (1st, 2nd or 3rd).</p><p><strong>Results: </strong>No interaction was observed between supplementation and parturition for any of the analyzed variables. The dietary supplementation of purified beta-glucans did not influence the reproductive performance of sows, litter performance and the immune response of 1st, 2nd or 3rd parity sows during gestation and lactation. Third parity sows had heavier litters at farrowing, greater weight gain, higher colostrum Brix value and serum immunoglobulin G than 1st and 2nd parity sows.</p><p><strong>Conclusion: </strong>It is concluded that dietary supplementation of beta-glucans for gestation and lactation sows of 1st, 2nd or 3rd parity did not affect performance of sows and litters; however, 3rd parity sows had heavier litters, greater weight gain at weaning, higher values of colostrum Brix and serum immunoglobulin G than 1st and 2nd parity sows.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a multi-strain probiotic on rabbits productive traits, antioxidant defence, caecal microbiota and short‑chain fatty acids, and intestinal histomorphology.","authors":"Vincenzo Tufarelli, Caterina Losacco, Gianluca Pugliese, Alessandra Tateo, Michele Schiavitto, Fabrizio Iarussi, Vito Laudadio, Letizia Passantino","doi":"10.5713/ab.24.0716","DOIUrl":"https://doi.org/10.5713/ab.24.0716","url":null,"abstract":"<p><strong>Objective: </strong>This paper aimed to evaluate the effects of a multi-strain probiotic as natural feed additives on the productive performance, blood parameters, antioxidant defence, caecal short‑chain fatty acid profile as well as the effectiveness on the intestinal morphology and on the equilibrium modification of caecal microbiota of growing rabbits.</p><p><strong>Methods: </strong>Eighty-six-week-old Italian White rabbits were assigned into two dietary groups: the control group was fed without any additive, while the test group received a diet supplemented with a multi-strain probiotic (MS-Prob; Slab51®) at a dosage of 250 mg/kg diet. The feeding trial lasted up to 12 weeks of age.</p><p><strong>Results: </strong>Rabbits MS-Prob diet had significantly higher daily and final body weight recording also the best of feed efficiency compared to control group. Rabbits in MS-Prob group showed improved slaughter weight and carcass dressing yield. No significant effect was found on meat protein, lipids and ash contents. Serum total cholesterol, low-density lipoprotein (LDL), high-density lipoprotein (HDL) and triglycerides decreased significantly in rabbits fed the test-diet. In rabbits fed MS-Prob, the activity of glutathione peroxidase (GPx), glutathione S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD) increased significantly, whereas the level of thiobarbituric acid-reactive substance (TBARS) decreased significantly. Caecal pH, ammonia-N and total volatile fatty acids (VFAs) were not significantly influenced by dietary treatments. Conversely, VFAs molar proportions were statistically affected by diets, with higher acetic and butyric acid concentrations in caecum of rabbits fed MS-Prob. Feeding of MS-Prob reduced harmful bacterial growth (Escherichia coli, Bacillus spp., Clostridium spp.) while promoting beneficial bacteria (Lactobacillus spp., Bacteroides spp.). Moreover, MS-Prob positively influences rabbit gut histomorphology, showing higher villus height, villus width, and crypt depth.</p><p><strong>Conclusion: </strong>s: This study indicated that MS-Prob (Slab51®) supplementation stimulated the performance of growing rabbits and positively affected blood serum parameters, intestinal morphology, and caecal environment and microbiota.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary nano-selenium and sodium selenite on the milk profiles of selenoamino acids, selenoenzymes, and selenoproteins of dairy cows.","authors":"Xiaoyu Ji, Xuejuan Deng, Ning Liu, Jianping Wang","doi":"10.5713/ab.24.0571","DOIUrl":"https://doi.org/10.5713/ab.24.0571","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the effect of dietary nanoselenium (nanoSe) and sodium selenite (SS) on the concentrations of selenoamino acids, the activities of selenoenzymes, and the mRNA expressions of selenoproteins in the milk of peripartal dairy cows.</p><p><strong>Methods: </strong>Three diets included a control (basal diet) with background selenium at 0.06 mg/kg and treatments with either SS or nanoSe added at the same selenium concentration of 1.00 mg/kg of diet. A total of 45 dairy cows were randomly allocated to three groups. The feeding trial lasted for 42 days from prenatal 21 days to postnatal 21 days.</p><p><strong>Results: </strong>NanoSe increased (p<0.05) milk yield compared to the control and SS. In milk, both SS and nanoSe increased (p<0.05) the concentrations of selenium, selenocysteine, and selenomethionine, and the activities of glutathione peroxidase 4, glutathione reductase, thioredoxin reductase, glutathione S-transferase, and iodothyronine deiodinases (Type 2 and 3). The nanoSe showed higher (p<0.05) effects on these parameters than SS. Also, dietary nanoSe upregulated (p<0.05) the mRNA expressions of selenoproteins P, W, S, F, M, N, K, O, H, and I in milk compared to the control. For most selenoproteins, there was no difference between SS and nanoSe, only selenoprotein K was higher (p<0.05) in nanoSe than in SS.</p><p><strong>Conclusion: </strong>In conclusion, dietary nanoSe increased milk yield, milk selenoamino acids and selenoproteins in peripartal dairy cows.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The expression pattern of butyric acid transporter in the large intestine with growth and development of suckling lambs.","authors":"Jian Ma, Tao Li, Lu Lin, Chunmei Du, Chen Wei, Fuquan Yin, Xuemei Sun, Gang Lyu, Shangquan Gan","doi":"10.5713/ab.24.0490","DOIUrl":"https://doi.org/10.5713/ab.24.0490","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this research was to explore the changes of morphological parameters, short chain fatty acid contents and butyric acid transporter expression levels in the large intestine with growth and development of sucking lambs.</p><p><strong>Methods: </strong>A total of 48 newborn male Hu sheep lambs (body weight = 2.94 ± 0.22 kg) were selected in this experiment. At 0, 7, 14, 28 and 42 days of ages, 6 lambs were slaughtered to collect cecal and colonic samples to analyze morphological parameters, short chain fatty acid contents and butyric acid transporter mRNA expression.</p><p><strong>Results: </strong>The organ index of the cecum in d 0, 28 and 42 groups was higher (p<0.05) than that in d 7 and 14 groups. Compared with other age groups, the organ index of the colon was significantly increased (p<0.05) at d 42 group. After 7 days of age, the contents of acetic, propionic and butyric acids in the cecum and colon were significantly increased (p<0.05) as age increasing. A similar trend of mRNA expressions of butyric acid transporters, including monocarboxylate transporter, sodium-coupled monocarboxylate transporter, sodium-hydrogen exchanger, down regulated in adenoma, putative anion transporter-1 and anion exchanger-2, was found. In the cecum, the Escherichia coli count in d 14 group was higher (p<0.05) than that in other age groups, whereas an opposite tendency was found in Lactobacillus count between d 14 and other groups. Additionally, in the cecum and colon, the relative expression of claudin-1 in d 14 group was lower (p<0.05) that in d 42 group.</p><p><strong>Conclusion: </strong>Overall, the current results indicate that the expression levels of butyric acid transporters in the cecum and colon of lambs are significantly influenced by days of age.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of individual and combined doses of added nitrite and nitrate on the quality of dry-fermented sausage.","authors":"Suk Nam Kang, Donggyun Yim","doi":"10.5713/ab.24.0561","DOIUrl":"https://doi.org/10.5713/ab.24.0561","url":null,"abstract":"<p><strong>Objective: </strong>We aimed to investigate the impacts of nitrate and nitrite on the salami fermentation process.</p><p><strong>Methods: </strong>Experimental groups of dry-fermented sausage were prepared with 100 ppm nitrite (T1), 150 ppm nitrite (T2), and 100 ppm nitrite + 50 ppm nitrate (T3). Salami quality was evaluated after a 30-day fermentation-drying process.</p><p><strong>Results: </strong>There were no significant differences in residual nitrite levels between T1 and T2, while the T3 samples exhibited the highest residual nitrite. T2 (150 ppm nitrite) showed a significantly higher pH value compared to T1 (100 ppm nitrite). Water activity was lowest in the T2 sample. T1 had a higher TBARS value than other treatments. In the color analysis, the L* values of T1 and T2 were significantly higher than those of T3, whereas the a* values were significantly higher in T3. In the texture analysis, T3 exhibited a higher shear force than T1 and T2. In the microbial analysis, adding 50 and 100 ppm of nitrite did not reduce the total plate count (TPC) and lactic acid bacteria count. However, T3 had a lower TPC than T1 and T2.</p><p><strong>Conclusion: </strong>The results suggest that different levels of nitrite/nitrate affect salami quality. This study is a valuable resource for understanding how using nitrite and nitrate alone or in combination affects quality in salami production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of subclinically hypocalcemic stress on the plasma metabolomic profile of dairy goats.","authors":"Jia Zhang, Dan Yang, Yafei Zeng, Kaini Guo, Jiajian Zhang, Yan Huang, Yuzhen Sui, Qimin Liu, Xiaoxuan Mo, Chenxu Zhao, Jianguo Wang","doi":"10.5713/ab.24.0567","DOIUrl":"https://doi.org/10.5713/ab.24.0567","url":null,"abstract":"<p><strong>Objective: </strong>Subclinical hypocalcemia induces metabolic stress, which adversely affects the production performance of peripartal dairy goats. This study sought to uncover new aspects of disease prevention and control by applying metabolomics and lipidomics to identify key differences in metabolite expression between affected and healthy animals.</p><p><strong>Methods: </strong>This study collected plasma samples from 96 primiparous Guanzhong dairy goats with similar body condition scores (2.75 ± 0.15, mean ± standard deviation) on the day of calving, aiming to uncover new aspects of disease prevention and control by applying metabolomics and lipidomics to identify key differences in metabolite expression between affected and healthy animals.</p><p><strong>Results: </strong>The study identified 23 differential metabolites and 30 differentially altered lipids involved in various metabolic pathways, such as phenylalanine and tyrosine metabolism, aminoacyl-tRNA biosynthesis, and glycerophospholipid metabolism. Our research revealed significant differences in the regulation of calcium-related hormones and associated metabolites between subclinical hypocalcemic and healthy dairy goats. Specifically, we found a positive correlation between parathyroid hormone (PTH) and aspartate aminotransferase (AST) in the healthy group, contrasting with a negative correlation observed in the subclinical hypocalcemic group.</p><p><strong>Conclusion: </strong>Additionally, phenylalanine and phosphatidylserine were identified as potential biomarkers indicative of subclinical hypocalcemia. These findings offer a novel approach to managing subclinical hypocalcemia in dairy goats, potentially transforming prevention and control strategies in the dairy goat industry.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of trehalose supplementation on ruminal microbiota and productivity of Japanese Black heifers under heat-stressed conditions.","authors":"Yasuhiro Morita, Akihisa Mukaiyama, Seiji Inoue, Kazuhisa Mukai, Shuichi Matsuyama, Satoshi Ohkura","doi":"10.5713/ab.24.0468","DOIUrl":"https://doi.org/10.5713/ab.24.0468","url":null,"abstract":"<p><strong>Objective: </strong>Hot environments negatively affect cattle productivity, and global warming also causes heat stress, thereby adversely impacting cattle production. Improving cattle management under such conditions is an urgent issue. Trehalose can ameliorate volatile fatty acid production and the diversity of ruminal microbiota in dairy cattle. However, no studies have evaluated on Japanese beef heifers. In this study, we investigated the effects of trehalose supplementation on the ruminal microbiota and productivity of Japanese Black heifers under heat-stressed conditions.</p><p><strong>Methods: </strong>Six cyclic Japanese Black heifers were divided into two groups: control and trehalose supplemented. The ruminal microbiota, pH, and volatile fatty acid production of these heifers were analyzed over 10 weeks in the summer in central Japan.</p><p><strong>Results: </strong>During the experimental period, the heifers in the control group showed significantly higher concentrations of ruminal acetic and propionic acids than those in the trehalose-supplemented group (p<0.05, two-way repeated measures ANOVA). The acetic/propionic acid ratio showed no significant difference between the two groups. The alpha diversity in the ruminal bacterial biota in the trehalose supplemented group was higher than that in the control group (p<0.05, two-way repeated measures ANOVA) along with a change in the beta diversity of the ruminal fungal biota (p<0.05, PERMANOVA). LEfSe analysis in ruminal microbiota identified specific microorganisms in the control and the trehalose-supplemented samples: 4 and 13 in bacteria; each one fungus; 5 and 4 protozoan families, respectively.</p><p><strong>Conclusion: </strong>Trehalose supplementation in the summer improved ruminal microbiota, especially the types of ruminal bacteria and fungi related to carbohydrate digestion, and maintained the balance of ruminal VFA production in Japanese Black heifers. Therefore, trehalose supplementation in feed could improve cattle production under heat-stressed conditions and in global-warming scenarios.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of stocking densities on growth, organ index, serum biochemistry, gut morphology and microbiota of young ducks in a rice-duck-crayfish coculture system.","authors":"Xiao Long He, Zhen Hua Liang, Ze Heng Huang, Yan Wu, Jia Liu, Tao Huang, Jing Bo Liu, Jin Song Pi, Hao Zhang","doi":"10.5713/ab.24.0488","DOIUrl":"https://doi.org/10.5713/ab.24.0488","url":null,"abstract":"<p><strong>Objective: </strong>The rice-duck-crayfish (RDC) coculture system, an ecologically efficient breeding method, supports ducks' natural habits and enhances duck welfare. However, the optimal stocking density and its influence on duck health in this system remains undetermined. The study examined the effects of stocking densities on growth, organ index, serum biochemistry, gut morphology and microbiota of ducks in RDC system.</p><p><strong>Methods: </strong>A total of five hundred and forty 20-day-old Nonghu No. 2 ducks were randomly divided into low-density (8 birds/666.67 m2, LD), medium-density (12 birds/666.67 m2, MD) and high-density (16 birds/666.67 m2, HD) groups, with three replicates in each group, and the symbiosis period was up to 40 days until rice tasselling.</p><p><strong>Results: </strong>There were no significant differences in final body weight, average daily gain, or feed:gain ratio between groups (p>0.05); the liver and spleen indices of ducks in HD group were significantly greater than in LD group (p<0.05); the serum albumin concentration in HD group decreased, whereas creatine kinase activity increased (p<0.05); the ileal crypt depth significantly increased; and the ileal villus height and villus/crypt ratio significantly decreased in ducks in MD and HD groups than in LD group (p<0.05). Additionally, the abundance of cecal Deferribacterota and Spirochaetota increased significantly (p<0.05), while the abundance of Firmicutes decreased significantly (p<0.05) with increasing stocking density. Moreover, the increase in stocking density significantly decreased the abundance of some beneficial bacteria (Faecalibacterium and Fournierella) and increased the abundance of some harmful bacteria (Mucispirillum and Brachyspira) (p<0.05).</p><p><strong>Conclusion: </strong>These preliminary results suggest that moderately high-density breeding doesn't significantly affect duck growth, but increased stocking density led to changes in cecal microbiota and dysbiosis. Reducing stocking density positively affects immune parameters and ileum morphology.However, due to the limited number of total replicates of the study, further research is needed to validate the reliability of the results.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of single nucleotide polymorphisms associated with litter size in goats using genotyping-by-sequencing and association analysis.","authors":"Satoshi Kubota, Thara Wongdee, Pramote Paengkoum","doi":"10.5713/ab.24.0533","DOIUrl":"https://doi.org/10.5713/ab.24.0533","url":null,"abstract":"<p><strong>Objective: </strong>Improving fertility is a key goal in goat production. This study aimed to detect single nucleotide polymorphisms (SNPs) associated with female goat reproductive performance for use in selection processes.</p><p><strong>Methods: </strong>Nine reproductive traits were evaluated, including litter size and age at the first, second, and third parities, as well as intervals between parities, in 31 female goats (2 purebred and 29 crossbred goats in various combinations of seven breeds). DNA was extracted from blood, and SNP data were obtained using the genotyping by sequencing (GBS) method. After filtering for allele depth and missing genotype data, the retained SNPs were subjected to population structure analysis and association analysis with the nine traits. For the association analysis, SNPs with false discovery rates ≤ 0.05 were considered significant. PCR allele competitive extension (PACE) genotyping assay was applied to develop genetic markers.</p><p><strong>Results: </strong>An average of 304,852 SNPs were initially detected in the 31 female goats. After filtering, 21,665 SNPs were retained. The first two principal components obtained from individual genotypes classified the 31 goats into three clusters. In the association analysis, six SNPs on four chromosomes were significantly associated with the litter size at first parity. The most significant SNP was detected on chromosome 4, and three genes-IKAROS family zinc finger 1 (IKZF1), fidgetin-like 1 (FIGNL1), and dopa decarboxylase (DDC)-were found within 100 kb downstream and upstream of the SNP. The PACE genotyping assay confirmed genotypes at this SNP with a 96% concordance rate.</p><p><strong>Conclusion: </strong>SNPs significantly associated with litter size at first parity, candidate genes, and the PACE genotyping methods applied in this study can be used for selecting female goats in future genetic improvement programs. However, further study on the frequency of genetic mutation with a larger sample size and functional studies of the candidate genes are required.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}