Animal Bioscience最新文献

{"title":"Functional characterization of porcine septin12 and its role in male reproduction.","authors":"Pei Wang, Xia Zhang, Hailong Huo, Luogang Li, Feidi Wen, Shuyan Wang, YongYun Zhang, Renaguli Turgong, Jinlong Huo","doi":"10.5713/ab.250538","DOIUrl":"10.5713/ab.250538","url":null,"abstract":"<p><strong>Objective: </strong>The objective is to identify and functionally characterize the pig septin12 gene, focusing on its expression pattern, subcellular localization, and interacting proteins, in order to explore its potential roles in male reproduction.</p><p><strong>Methods: </strong>Full-length septin12 cDNA was obtained from the testis of the Banna mini-pig inbred line (BMI) using rapid amplification of cDNA ends. Quantitative real-time polymerase chain reaction and western blot were employed to assess tissue-specific expression in BMI pigs. Subcellular localization was determined by fluorescence microscopy following transfection of EGFP-tagged septin12. A yeast two-hybrid (Y2H) screen using an adult BMI testis cDNA library identified potential interacting proteins, which were then validated by Co-immunoprecipitation (Co-IP) and immunofluorescence (IF). Gene Ontology (GO) analysis was performed to ascertain the functional categories of the interactors.</p><p><strong>Results: </strong>The cloned septin12 cDNA from the BMI testis was 1,289 base pairs (bp) in length. septin12 mRNA and protein expression was predominantly observed in the BMI testis, suggesting its critical role in male reproductive function. septin12 localized to both fibrous filamentous and punctate cytoplasmic structures. Thirteen interacting proteins were identified by Y2H, including ATAD5, ATP1B3, CENPL, ENO1, and septin5, with GO analysis indicating enrichment in mitotic nuclear division, cell growth, and G2/M phase transition. Furthermore, Co-IP and IF confirmed interactions between septin12 and electron transfer flavoprotein subunit alpha (ETFA) as well as lactate dehydrogenase B (LDHB), supporting the results of the Y2H screen.</p><p><strong>Conclusion: </strong>This study identified and characterized the septin12 gene in BMI pig, highlighting its predominant expression in the testis and implicating its role in male reproductive function. Protein interaction analysis uncovered partners involved in cell division and metabolism, suggesting potential mechanisms by which septin12 may influence spermatogenesis. Co-IP and IF further validated interactions with ETFA and LDHB. These findings offer a groundwork for future investigations on the role of septin12 in male fertility.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"250538"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147607599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dihydroorotate dehydrogenase inhibition and vitamin E supplementation on ferroptosis-related mechanisms and post-mortem meat quality in broiler chickens.","authors":"Tomonori Nakanishi, Saki Nemoto, Laurie Erickson, Satoshi Kawahara","doi":"10.5713/ab.250847","DOIUrl":"10.5713/ab.250847","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the role of ferroptosis, an iron-dependent form of regulated cell death, in meat quality by comparing the effects of pre-slaughter administration of teriflunomide, an inhibitor of dihydroorotate dehydrogenase (DHODH), which can induce ferroptosis, and dietary vitamin E, an antioxidant that can suppress ferroptosis, on the post-mortem biochemical properties of broiler chicken muscle.</p><p><strong>Methods: </strong>Broiler chickens were randomly allocated to three groups: control, teriflunomide-treated, and vitamin E-supplemented. Teriflunomide was administered subcutaneously prior to slaughter, and vitamin E was provided in the diet. Post-mortem thigh muscles were analyzed for oxidative status, such as lipid peroxide levels and the ratio of reduced to oxidized glutathione (GSH/GSSG); the expression of ferroptosis-related genes, such as glutathione peroxidase 4 (GPX4) and acyl-CoA synthetase long-chain family member 4 (ACSL4); and meat quality traits such as pH, color, and water-holding capacity.</p><p><strong>Results: </strong>Teriflunomide significantly reduced the GSH/GSSG ratio (p<0.001) and increased ACSL4 expression compared with the control and vitamin E groups (p<0.05), indicating elevated oxidative stress. Vitamin E significantly increased GPX4 expression compared with teriflunomide (p<0.05). Lipid peroxidation was numerically lower in the vitamin E group than in the other groups, although the differences were not statistically significant (p = 0.062). Teriflunomide slowed post-mortem pH decline (p<0.001) and decreased L* and b* values, whereas a* values were higher (p<0.05). Vitamin E maintained higher L* and b* values and slightly lower a* values than the control (p<0.05). No statistically significant differences were observed in water-holding capacity among the groups.</p><p><strong>Conclusion: </strong>These findings indicate that ferroptosis-related processes play a critical role in regulating post-mortem meat quality in broiler thigh muscles. DHODH inhibition by teriflunomide and antioxidant treatment with vitamin E yielded contrasting effects.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"250847"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of lipid oxidation in refrigerated beef using multilayer encapsulated chitosan/gum arabic/polydextrose single-bulb garlic antioxidant.","authors":"Vivi Nuraini, Anang Mohamad Legowo, Sri Mulyani, Retno Adiwinarti","doi":"10.5713/ab.250743","DOIUrl":"10.5713/ab.250743","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to evaluate the effectiveness of multilayer encapsulation of single-bulb garlic extract using a chitosan/gum arabic/polydextrose (MESG) system as a natural antioxidant in inhibiting oxidative damage in beef steak stored at refrigerator temperatures.</p><p><strong>Methods: </strong>The study was designed using a completely randomized factorial design. The first factor was the concentration of MESG (MESG-0%, MESG-4%, and MESG-8%), and the second factor was the observation day (day 0, 3, 6, 9, 12, and 15). The marinated meat samples were stored at chiller temperature (6°C) for 15 days, and then the samples were analyzed for antioxidant activity, thiobarbituric acid reactive substances (TBARS) values, free fatty acid (FFA), pH, and fatty acid profile.</p><p><strong>Results: </strong>Physical analysis results indicated that the MESG displayed a complex, porous structure of MESG. The addition of MESG had a positive effect on the TBARS value, FFAs, and pH. Compared to the control (MESG-0%), MESG-4% and MESG-8% presented significantly (p<0.05) lower FFA levels and TBARS, while maintaining the stability of C20:4 ω6 (arachidonic acid) and C20:2 (eicosadienoic acid) during a 15-day storage period. The results showed a potential protective effect of the encapsulation method against lipid oxidation during refrigerated storage.</p><p><strong>Conclusion: </strong>This encapsulation technology is a strategic approach for preserving natural antioxidant quality, thereby extending shelf life and maintaining the quality of meat products, while also providing a viable alternative to synthetic preservatives.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"250743"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147607597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional evaluation of Zanthoxylum bungeanum leaves and their effects on reproductive performance, milk composition, antioxidant status, and gut microbiota in sows.","authors":"Hanshu Lin, Shanchuan Cao, Heng Yin, Jianfei Zhao, Jingbo Liu","doi":"10.5713/ab.250892","DOIUrl":"10.5713/ab.250892","url":null,"abstract":"<p><strong>Objective: </strong>Two experiments were conducted to investigate the digestible energy of Zanthoxylum bungeanum leaves (ZBL) and effects on sow reproductive performance.</p><p><strong>Methods: </strong>In Exp 1, 24 sows (parity 3) on d 60 of gestation were used in a 10-dfecal collection study to determine the digestible energy of ZBL. Exp 1 comprised two dietary treatments: a basal diet and a 10% ZBL diet. In Exp 2, 60 sows (parity 3) on d 30 of gestation were used to evaluate reproductive performance and related indices. Exp 2 comprised two dietary treatments: a basal diet (CON group) and a 4% ZBL diet (ZBL group). The lactation period was twenty-eight days. Microbial samples were collected on farrowing day.</p><p><strong>Results: </strong>The digestible energy of ZBL was 13.77 MJ/kg. Dietary supplementation with 4% ZBL had no effect on litter size, number of live-born piglets, number of healthy piglets, litter weight at birth, or individual birth weight in sows. Milk fat concentration was higher in the ZBL group than in the CON group (p<0.05). Serum blood urea nitrogen was elevated on lactation day in the ZBL group (p<0.05), and glucose concentration was higher on weaning day in the ZBL group than in the CON group (p<0.05). Total antioxidant capacity increased on lactation day (p<0.05) and weaning day (p<0.05), while malondialdehyde decreased (p<0.05) on lactation day in the ZBL group than in the CON group. At the genus level, 4% ZBL elevated (p<0.05) the relative abundances of Escherichia-Shigella, Cellulosilyticum, norank_f_Erysipelotrichaceae, Fecalibacterium, Adlercreutzia and Akkermansia, and reduced (p<0.05) those of Lachnospiraceae_XPB1014_group, Rikenellaceae_RC9_gut_ group, Prevotellaceae_UCG-001 and Bacteroides.</p><p><strong>Conclusion: </strong>The digestible energy of ZBL for sows was 13.77 MJ/kg. Dietary supplementation with 4% ZBL partially enhanced antioxidant status and modulated fecal microbiota, but had no effect on the sow reproductive performance.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"250892"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary crude glycerol as an energy source in broiler chickens: a meta-analysis.","authors":"Rasheed Olayiwola Sulaimon, Ridwan Olalekan Oyeniyi, Yusup Sopian, Katatikarn Sahatsanon, Anuraga Jayanegara, Panneepa Sivapirunthep, Pattraphorn Patthararangsarith, Chanporn Chaosap","doi":"10.5713/ab.250686","DOIUrl":"10.5713/ab.250686","url":null,"abstract":"<p><strong>Objective: </strong>This meta-analysis evaluated the effects of dietary crude glycerol (CG) supplementation on growth performance, carcass traits, meat quality, and blood biochemical parameters in broiler chickens.</p><p><strong>Methods: </strong>A systematic search of peer-reviewed studies in Scopus, ScienceDirect, and Google Scholar was performed following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses protocol. Twenty-five eligible studies were included. Effect sizes were calculated as standardized mean differences using a random-effects model in Open-MEE software. Subgroup analyses were performed according to strain, sex, rearing phase, and inclusion level.</p><p><strong>Results: </strong>CG supplementation increased body weight gain (p<0.05) and improved feed conversion ratio (p<0.05), while feed intake was unaffected. Among carcass traits, breast yield increased (p<0.05), whereas carcass yield and thigh yield were not affected. CG inclusion also reduced meat ash content and ultimate pH, while drip loss increased, indicating potential negative effects on water-holding capacity. Blood biochemical indices, including reduced alanine aminotransferase and elevated alkaline phosphatase, indicated adaptive physiological responses without evidence of toxicity. Subgroup analyses revealed that strain, sex, and inclusion level moderated growth outcomes (p<0.05).</p><p><strong>Conclusion: </strong>CG can be incorporated into broiler diets as an alternative energy source to enhance growth performance without compromising carcass yield. However, its influence on meat quality highlights the need to optimize inclusion levels and consider variability among glycerol by-products before large-scale application.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"250686"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of chemically protected sodium butyrate on growth performance, serum indicators, rumen fermentation parameters, and microbiota of Gangba sheep.","authors":"Yining Xie, Mebratu Melaku, Zhaohan Zhan, Xiaokang Jing, Junhong Wang, Huaibao Zhao, Bao Yi, Teng Ma, Liang Chen, Hongfu Zhang","doi":"10.5713/ab.250681","DOIUrl":"10.5713/ab.250681","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the effects of chemically protected sodium butyrate (CSB) on growth performance, serum indicators, rumen fermentation parameters, and microbial communities in Gangba sheep.</p><p><strong>Methods: </strong>Twenty-four healthy 5-month-old male Gangba sheep with an initial body weight of 19.54±1.04 kg were randomly assigned to four groups and fed diets containing 0, 1.0, 5.0, and 10.0 g/kg CSB for 74 days. After a 14-day adaptation period, the daily feed intake of Gangba sheep was measured. Fecal samples were collected during the last 7 days of the trial, and serum and rumen fluid were sampled on the final day.</p><p><strong>Results: </strong>CSB improved final body weight, average daily dry matter intake, and average daily gain while reducing the feed conversion ratio of Gangba sheep (p<0.05). In addition, CSB improved the apparent digestibility of crude protein, dry matter, and neutral detergent fiber (p<0.05). Furthermore, CSB increased serum immunoglobulin G levels while decreasing tumor necrosis factor-α levels (p<0.05). Meanwhile, CSB increased serum growth hormone-releasing hormone levels (p<0.05). Furthermore, CSB increased rumen fluid pH and concentrations of ammonia nitrogen, acetic acid, iso-butyric acid, butyric acid, and total volatile fatty acids, as well as activities of lipase and butyrate kinase (p<0.05). Besides, CSB enhanced the rumen microbiota structure by increasing the relative abundance of Fibrobacter, unclassified Oscillospiraceae, and Ruminococcus.</p><p><strong>Conclusion: </strong>CSB improved immunological and hormone indicators, enhanced the rumen ecological environment, and increased feed digestibility and utilization, thereby promoting the growth of Gangba sheep. It is recommended to use a dosage of 5.0 g/kg dry matter for optimal growth performance and health benefits.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"250681"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of drying method and various level of chickpea powder on the quality characteristics of emulsified model sausages.","authors":"Min Jae Kim, Koo Bok Chin","doi":"10.5713/ab.260060","DOIUrl":"10.5713/ab.260060","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the effects of chickpea powder (CPP) drying method (freeze-drying [FD] and oven-drying [OD]), and concentration (1.0% and 1.5%) on the quality characteristics of CPP-containing emulsified model sausages (EMSs), using a conventional emulsion-type meat system. CPP was assessed as a plant-based alternative to soy protein isolate (SPI), which is commonly used in meat formulations.</p><p><strong>Methods: </strong>CPPs were prepared by FD or OD at 60°C and incorporated into EMSs at 1.0% and 1.5%. A control without added protein (CTL) and an SPI-containing reference (REF) were included. Product pH, color, cooking loss (CL), expressible moisture (EM), proximate composition, texture profile parameters, protein solubility, surface hydrophobicity, and sulfhydryl group levels were analyzed. In addition, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and scanning electron microscopy were performed.</p><p><strong>Results: </strong>CPP addition affected the pH, water-holding capacity, texture, and color of EMSs. Among the treatments, 1.5% oven-dried CPP showed the highest pH, lowest CL and EM, and the most improved textural properties. Redness decreased, whereas yellowness increased with CPP concentration, particularly in oven-dried CPP treatments. Fat content was unchanged, and protein content showed only minor differences. At 1.5%, CPP improved protein functionality by increasing protein solubility and reducing surface hydrophobicity and sulfhydryl groups, suggesting enhanced protein dispersion and cross-linking. Microstructural analysis confirmed a denser protein matrix with smaller pores and embedded starch granules. Overall, CPP-treated EMSs showed comparable or superior performance to SPI-treated reference EMSs.</p><p><strong>Conclusion: </strong>Both the drying method and concentration of CPP markedly affect EMS quality. Oven-dried CPP at 1.5% was especially effective in enhancing water retention and textural integrity, which was associated with improved protein functionality and microstructural stabilization, implying its usability as a functional plant-based protein ingredient in conventional emulsified meat products, expanding its applicability beyond reduced-fat systems.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"260060"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ASAP1 gene InDel variants are associated with enhanced goat resistance against Brucella infection.","authors":"Xiaoyu Liu, Congliang Wang, Xiaoyuan Ren, Zhaofei Ren, Yanyan Li, Wangye Liu, Rongrong Li, Xiaoyue Song, Helin Li, Lei Zhang, Shenghui Chen, Xiaomin Du, Jinlian Hua, Haijing Zhu","doi":"10.5713/ab.250722","DOIUrl":"10.5713/ab.250722","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the potential of the ASAP1 gene as a genetic biomarker for brucellosis resistance/susceptibility in goats.</p><p><strong>Methods: </strong>This study collected samples from female Shaanbei white cashmere (SBWC) goats to investigate the association between the ASAP1 gene and brucellosis susceptibility. Peripheral blood mononuclear cells (PBMCs) were isolated from goats with various haplo types and Brucella statuses, and the association was evaluated using polymerase chain re action (PCR), quantitative reverse transcription (qRT)-PCR, and lipopolysaccharide (LPS) stimulation assays.</p><p><strong>Results: </strong>The ASAP1 gene was expressed most in th e spleen, significantly more than in the kidney and heart (p<0.05). In the SBWC goats population, three genotypes insertion/insertion (II), insertion/deletion (ID), and deletion/deletion (DD) were identified at the P2, P5, and P7 sites of goat ASAP1 gene. Association analysis showed that P2 and P7 sites variants were associated with host resistance to Brucella infection with the II genotype used as reference (p<0.05; p<0.01) and maintained after multiple testing correction. The ASAP1 gene was observed lower expression in testicular tissues of Brucella-infected adult SBWC goats compared to healthy controls (p<0.01). Haplotype analysis revealed Hap3 and Hap5 were associated with brucellosis-resistant compared to Hap1 (p<0.05). PBMCs were isolated from goats carrying Hap1, Hap3, and Hap5. After LPS stimulation, significantly reduced ASAP1 expression was detected in the susceptible haplotype Hap1 compared to the resistant haplotypes. The highest expression level was exhibited by the most resistant haplotype, Hap5. Furthermore, resistant haplotypes showed more rapid activation of key inflammatory pathways and pro-inflammatory cytokines (NF-κB, IL-6, TNF-α, IFN-γ) compared to susceptible Hap1, with faster resolution of the inflammatory response observed, particularly in the most resistant haplotype Hap5.</p><p><strong>Conclusion: </strong>The present study demonstrates that ASAP1 gene InDel variants influence brucellosis resistance in SBWC goats, providing a theoretical basis for breeding resistant populations.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"250722"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of composite probiotics on growth performance, diarrhea rate, and serum indicators in newborn Holstein calves.","authors":"Kun Yang, Na Qian, Yuanyuan Xing, Min Gao, Dabiao Li","doi":"10.5713/ab.250717","DOIUrl":"10.5713/ab.250717","url":null,"abstract":"<p><strong>Objective: </strong>The present study aimed to determine the effects of compound probiotics on growth performance, diarrhea incidence, fecal pathogenic microorganisms, and serum parameters in newborn calves.</p><p><strong>Methods: </strong>A total of 48 healthy newborn calves with similar body weights (BWs; mean±standard deviation: 37.0±2.0 kg) were randomly assigned to three groups (n = 16 per group). Control group (CON), yeast group (CP1), Bacillus licheniformis+yeast group (CP2). The feeding trial lasted for 60 days. Growth performance, diarrhea incidence, fecal scores, and serum parameters were assessed on days 1, 30, and 60 of the trial, while fecal samples obtained on days 15 and 30 were analyzed for pathogenic microorganism positivity.</p><p><strong>Results: </strong>Compared with CON group, calves in the CP2 group had higher BW (day 30; p<0.05), average daily gain (days 1-30; p<0.05), and feed efficiency (days 1-30; p<0.05). Compared with CON group, the incidence of diarrhea and fecal scores (days 1-60) were lower in both CP1 and CP2 groups (p<0.05). Fecal samples from these groups also exhibited reduced positive rates of pathogenic microorganisms (rotavirus, coronavirus, cryptosporidium). Notably, compared with CON group, serum antioxidant and immune markers, total antioxidant capacity, superoxide dismutase, and immunoglobulin A levels were elevated (p<0.05), whereas malondialdehyde and tumor necrosis factor-alpha levels were decreased (p<0.05), and serum diamine oxidase levels were also reduced in the CP1 and CP2 groups (p<0.05). Furthermore, compared with the CON group, calves in the CP2 group exhibited lower serum cortisol and higher growth hormone (GH) levels (p<0.05). No interactions between treatment and period were observed.</p><p><strong>Conclusion: </strong>The combination of Bacillus licheniformis and yeast exerts promotes newborn calves' growth and development, reduces diarrhea incidence, enhances antioxidant and immune functions, and improves overall health.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"250717"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of physicochemical and textural properties of emulsified pork model sausages treated with Rhynchosia nulubilis powders from different drying methods.","authors":"Min Jae Kim, Koo Bok Chin","doi":"10.5713/ab.251016","DOIUrl":"10.5713/ab.251016","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the effects of Rhynchosia nulubilis powder (RNP) processed by different drying methods on the physicochemical and textural properties of emulsified pork model sausages (EPMSs).</p><p><strong>Methods: </strong>RNP was prepared by freeze-drying (FP), oven-drying (OP), or obtained as a commercial powder (CP), and incorporated into EPMSs at levels of 1.0% and 3.0%. A control with no additional protein (CTL) and a reference formulation containing soy protein isolate (REF) were used for comparison. Product pH, color, water-holding capacity (cooking loss and expressible moisture), proximate composition, and texture profile parameters were evaluated.</p><p><strong>Results: </strong>RNP addition altered pH, color, water-holding capacity, and textural characteristics of EPMSs. FP- and OP-treated samples exhibited reduced cooking loss and expressible moisture compared with CTL and CP, indicating improved water-holding capacity, particularly at the 3.0% level. Redness decreased, while yellowness increased with RNP addition, depending on the processing method and concentration. Textural properties, including hardness, cohesiveness, gumminess, and chewiness, were enhanced in FP and OP treatments, whereas CP showed limited improvements.</p><p><strong>Conclusion: </strong>The results demonstrate that RNP processed by FP or OP effectively enhances the quality and functional properties of EPMSs. Properly processed RNP may serve as a promising non-meat protein ingredient for improving water retention and texture in processed meat products.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"251016"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353150/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}