Animal Bioscience最新文献

{"title":"Effect of active and heat-killed Clostridium butyricum on in vitro gas production, ruminal fermentation, and microbiota varying with pH levels.","authors":"Xinlong Zhang, Zhiyue Zhang, Hongxu Zhu, Guanghui Hu, Hangshu Xin, Jincheng Liu, Xu Lin, Xiaolai Xie, Peixin Jiao","doi":"10.5713/ab.24.0913","DOIUrl":"https://doi.org/10.5713/ab.24.0913","url":null,"abstract":"<p><strong>Objective: </strong>: The objective of this study was to evaluate the effects of inactive (heat-killed) Clostridium butyricum (ICB) on gas production kinetics, fermentation parameters, and microbiota with varying media pH levels in batch culture.</p><p><strong>Methods: </strong>: The in vitro experiment was designed as a completely randomized factorial arrangement, with 2 media pH levels (5.8 and 6.5) × 2 CB products (active and inactive) × 4 dosages. Two lactating dairy cows with ruminal fistulas, fed a diet comprising 40% forage and 60% concentrate, served as donors for rumen inoculum. Following 24 h of incubation, the gas production, dry matter disappearance (DMD), volatile fatty acid (VFA), ammonia nitrogen (NH3-N) and microbial profile were analyzed to determine the effect of treatment on fermentation.</p><p><strong>Results: </strong>: The gas volume (GV), DMD, total VFA concentration, NH3-N concentration, acetate proportion and microbial alpha diversity were inhibited when the media pH decreased from 6.5 to 5.8. Increasing the supplemental doses of ICB linearly increased the GV, DMD (trend) and butyrate proportion at media pH 6.5. Moreover, the increasing supplemental dose of active Clostridium butyricum (ACB) linearly increased GV, butyrate proportion and NH3-N concentration (trend) regardless of media pH, and linearly increased DMD, total VFA concentration and A:P ratio at media pH 6.5. Supplementing ICB decreased the relative abundance of Actinobacteria and Butyrivibrio in the fermentation fluid.</p><p><strong>Conclusion: </strong>s: Increasing media pH would favor rumen fermentation and alter bacterial community. Although both ACB and ICB have the potential to stimulate rumen fermentation in a dose-dependent manner, their effects vary with different media pH levels. Moreover, both ACB and ICB rarely caused changes to the rumen bacterial community.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of Germ Cell-like Cells from Dazl-eGFP Knock-In Chicken Somatic Cells via Transgenic Expression of Pluripotency and Germ Cell-Specific Transcription Factors.","authors":"Bo Ram Lee, Hyeon Yang, Sun Keun Jung, Sung June Byun, Tae Sub Park","doi":"10.5713/ab.25.0233","DOIUrl":"https://doi.org/10.5713/ab.25.0233","url":null,"abstract":"<p><strong>Objective: </strong>: Germ cell identity is orchestrated by the coordinated action of multiple key transcription factors during embryonic development, involving the induction and regulation of germline-specific gene expression. In chickens, the expression of DEAD-box helicase 4 (DDX4) and Deleted in Azoospermia-like (DAZL) genes plays a pivotal role in germplasm formation and the specification of germ cell lineage from a totipotent genome. This study aimed to investigate the regulatory mechanisms underlying germ cell fate determination.</p><p><strong>Methods: </strong>: We performed large-scale gene expression profiling to screen and select critical transcription factors. Through this analysis, we identified differentially expressed genes (DEGs) in chicken primordial germ cells (PGCs), including 1,020 transcription factors. Additionally, we generated a chicken DF1 cell line with an enhanced green fluorescent protein (eGFP) reporter precisely knocked into the transcriptional start site of the DAZL gene using the CRISPR-Cas9 system, enabling real-time monitoring of DAZL expression during reprogramming.</p><p><strong>Results: </strong>: Through analysis of transcription factor binding sites within ~10 kb upstream regions of DDX4 and DAZL, we selected 10 candidate transcription factors for germ cell induction. Subsequently, the 10 transcription factors identified as regulators of germ cell identity were transduced into the DAZL-knock-in eGFP DF1 cells. This approach led to the successful induction of eGFP-expressing cells in vitro, driven by the endogenous DAZL promoter. We further characterized these cells to confirm their germ cell-specific properties.</p><p><strong>Conclusion: </strong>: Our findings offer new insights into the transcriptional regulation of chicken germ cells by identifying key factors responsible for the activation of DAZL expression. These results present valuable opportunities for advancing germ cell induction from somatic cells, with potential applications of in vitro models for studying germ cell-specific gene regulatory pathways in avian species.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural and crystal bundles disaggregated palygorskite in young broilers: A comparison study.","authors":"Zichao Tan, Yueping Chen, Chao Wen, Aiqin Wang, Yanmin Zhou","doi":"10.5713/ab.25.0150","DOIUrl":"https://doi.org/10.5713/ab.25.0150","url":null,"abstract":"<p><strong>Objective: </strong>: This study aimed to compare the effects of natural palygorskite (Nat-Pal) and crystal bundles disaggregated palygorskite (Dis-Pal) supplementation on the growth performance, intestinal health, and digestive function of young broilers.</p><p><strong>Methods: </strong>: A total of 400 male Arbor Acres broilers (one-day-old) were randomly assigned to five experimental groups with eight replicates over a 14-day experimental period. Chicks were fed a basal diet (Control group), the basal diet supplemented with 10 g/kg Nat-Pal or 2.5, 5, and 10 g/kg Dis-Pal, respectively.</p><p><strong>Results: </strong>: Dis-Pal linearly increased average body weight and average daily gain, and decreased feed to gain ratio of broilers from days 1 to 14, with the 5-10 g/kg Dis-Pal showing the most pronounced effects (P < 0.05). An addition of Dis-Pal linearly increased glucose level, and decreased triglyceride level in serum (P < 0.05). Compared to the control group, 5 g/kg Dis-Pal increased the digestibility of crude protein and dry matter, enhanced trypsin, lipase, and amylase activities in jejunal digesta, and elevated the ratio between villus height and crypt depth in jejunum and ileum (P < 0.05). Dis-Pal linearly increased ileal mucosal glutathione and total antioxidant capacity levels, as well as total superoxide dismutase and catalase activities, with 10 g/kg Dis-Pal addition showing the best effects (P < 0.05). The 2.5-10 g/kg Dis-Pal decreased the interferon-γ and tumor necrosis factor-α levels in ileal mucosa compared to the control group (P < 0.05). Moreover, Dis-Pal supplementation linearly increased interleukin-10, secretory immunoglobulin A, and immunoglobulin M levels, and decreased the interferon-γ level, quadratically increased total superoxide dismutase and catalase activities in jejunal mucosa (P < 0.05).</p><p><strong>Conclusion: </strong>: Dietary Dis-Pal supplementation could improve growth performance, intestinal health, and enhance the nutrients digestibility of young broilers. Supplementation with Dis-Pal was more advantageous for broilers than its Nat-Pal, and its optimal dosage was 5 g/kg.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome and microbiota analysis reveal differences in the cecum of weaning pigs in response to different dietary crude protein levels.","authors":"Yu-Hsiang Yu, Sheng-Bing Chen, Han-Tsung Wang, Chuan-Shun Lin, Andrzej Dybus, Beata Hukowska-Szematowicz, Yi-Hung Li","doi":"10.5713/ab.25.0135","DOIUrl":"https://doi.org/10.5713/ab.25.0135","url":null,"abstract":"<p><strong>Objective: </strong>: This study explored the effects of different dietary crude protein (CP) levels on the cecal transcriptome and microbial composition of weaning pigs.</p><p><strong>Methods: </strong>: A total of 96 weaning pigs were randomly assigned to 3 groups named the H, M, and L groups. The H, M, and L groups received 20% and 18%, 18% and 16%, and 16% and 14% CP in the early (1-14 days) and late phases (15-28 days), respectively.</p><p><strong>Results: </strong>: The final body weight and average daily gain were lower in the L group than in the other groups (p = 0.008). The feed conversion ratio was lower in the H and M groups than in the L group (p = 0.01). Cecal transcriptome analysis revealed that heatmap and principal component analysis of differentially expressed genes indicated the presence of distinct clusters between the groups. Genes involved in cell proliferation and differentiation and inflammation was downregulated in the M and L groups, compared with corresponding genes in the H group (p < 0.001). Pathway enrichment analysis suggested that genes related to IL-17 signaling pathway was downregulated in the M and L groups (p < 0.05). Beta diversity analysis and heatmap for microbial composition and function indicated the presence of distinct clusters between the groups. Carbohydrate-fermenting bacteria, such as Megasphaera elsdenii DSM 20460 and Blautia luti DSM 14534, were higher in the M and L groups compared with the H group (p ≤ 0.05). The abundance of Lactobacillus amylovorus DSM 20531 was higher in the M group than in the other groups (p ≤ 0.05). The abundance of L. amylovorus DSM 20531 was positively correlated with growth performance. Integrated multi-omics analysis suggested significant similarities between cecal transcriptome and microbiota (p < 0.01).</p><p><strong>Conclusion: </strong>: Reducing the level of CP modulate cell growth and alleviate inflammation in the cecum. A.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiomics reveals changes in lipid metabolism in the livers of landes geese before and after overfeeding.","authors":"Weiqing Ma, Liu Yang, Yadi Jing, Pengwei Ren, Xiang Liu, Meixia Zhang, Xiaomin Qi, Mingxia Zhu, Qiaomei Zhang","doi":"10.5713/ab.25.0405","DOIUrl":"https://doi.org/10.5713/ab.25.0405","url":null,"abstract":"<p><strong>Objective: </strong>: The aim of this experiment was to integrated production indices with omics sequencing to elucidate the systemic perturbations between hepatic metabolism and the gut microbiota during overfeeding.</p><p><strong>Methods: </strong>: A total of 120 seven-week-old male Landes geese were floor reared in a pen environment. Overfeeding commenced at week 8 using a corn-based diet containing 5% soybean oil. The feeding regimen consisted of three daily meals (150-180 g/meal) initially, gradually increasing to five meals (300-500 g/meal) after two weeks, and was maintained for a total overfeeding period of four weeks.</p><p><strong>Results: </strong>: The results demonstrated that overfeeding significantly increased liver weight and serum lipid levels, accompanied by intracellular lipid droplet accumulation. Concurrently, the downregulation of taurine-conjugated bile acids and the upregulation of free bile acids disrupted cholesterol homeostasis. Crucially, overfeeding triggered gut microbial dysbiosis characterized by Escherichia-Shigella enrichment and norank_o_Clostridia_UCG-014 depletion.</p><p><strong>Conclusion: </strong>: Our work demonstrated that the identification of the \"gut microbiota‒bile acid‒liver axis\" could as a pivotal signaling pathway driving overfeeding-induced foie gras formation while providing a theoretical foundation for overfeeding strategies to mitigate metabolic pathologies in waterfowl production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection index for beef cattle that maximizes overall growth yet constrains birth weight and other traits.","authors":"Kenji Togashi, Toshio Watanabe, Atsushi Ogino, Masakazu Shinomiya, Kazuhito Kurogi, Masanobu Nurimoto","doi":"10.5713/ab.24.0912","DOIUrl":"https://doi.org/10.5713/ab.24.0912","url":null,"abstract":"<p><strong>Objective: </strong>: Maximizing growth throughout the growth period is a practical goal in the beef industry. We developed a novel selection index, called the maximum growth index, that maximizes the growth throughout the growth process and simultaneously achieves the desired weight gain at designated time points by assigning arbitrary values to selection intensity.</p><p><strong>Methods: </strong>: We used a technique called Lagrange multipliers to maximize overall growth throughout the entire growth process and to restrict weight gains to desired amounts at specific times. We developed a selection index by applying random regression (RR) to the growth curve and using the genomically enhanced breeding values (GEBVs) of the RR coefficients as selection index traits.</p><p><strong>Results: </strong>: Examples of the developed index was applied based on assumed data in Japanese Black steers. Selection to maximize growth during the growth period and to moderate birth weight yielded an upwardly convex curve for weight gain during the growth process, and the peak daily gain was greater and earlier than achieved without this selection. Under a selection intensity of 0.5, the index that constrained birth weight to decrease by 2.5 kg achieved the pre-selection final weight 8 weeks earlier than occurred without this selection.</p><p><strong>Conclusion: </strong>: We developed a beef cattle selection index that maximized total weight gain during growth yet constrained birth weight and other traits. The maximum growth index that we developed enables reductions in birth weight concurrent with increases in final weight, thus benefiting the beef industry by increasing final weight and preventing dystocia.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing Precision Livestock Farming: Integrating Artificial Intelligence and Emerging Technologies for Sustainable Livestock Management.","authors":"Luis Orlindo Tedeschi, Pablo Guarnido Lopez, Hector M Menendez Iii, Seongwon Seo","doi":"10.5713/ab.25.0289","DOIUrl":"https://doi.org/10.5713/ab.25.0289","url":null,"abstract":"<p><p>Precision Livestock Farming (PLF) has evolved dramatically from basic monitoring systems to sophisticated artificial intelligence(AI)-driven decision support systems that enhance livestock management efficiency, sustainability, and animal welfare. This review examines the technological evolution of PLF since 2017, highlighting significant advancements in sensing technologies, computer vision, and artificial intelligence. Non-invasive technologies, including RGB-D cameras, 3D imaging systems, and IoT-enabled platforms, now capture detailed biometric and behavioral data in real time, while AI algorithms enable early disease detection, optimize feeding strategies, and improve reproductive management. Integrating these technologies with mechanistic models has created hybrid intelligent frameworks that address longstanding challenges in precision nutrition modeling. Future PLF development will likely focus on integrating large language models, adopting federated learning approaches to address data privacy concerns, and democratizing technologies for small-scale producers. Despite technological progress, challenges remain regarding data standardization, connectivity in rural environments, high implementation costs, and ethical considerations around increased animal monitoring. By fostering interdisciplinary collaboration among animal scientists, engineers, computer scientists, and social scientists, PLF can continue to drive sustainable and efficient practices in livestock production while ensuring that technologies complement rather than replace traditional husbandry knowledge.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of genes and lncRNAs for intramuscular and subcutaneous fat deposition in ducks by transcriptome analysis.","authors":"Tingting Zhou, Xunhao Meng, Wenshuang Liang, Min Xue, Tianqi Yang, Yong Jiang, Hao Bai, Guobin Chang, Guohong Chen, Zhixiu Wang","doi":"10.5713/ab.25.0268","DOIUrl":"https://doi.org/10.5713/ab.25.0268","url":null,"abstract":"<p><p>The purpose of this study was to identify key lncRNAs and mRNAs involved in fat deposition of meat ducks based on whole transcriptome sequencing for intramuscular preadipocyte (IMP-0), intramuscular adipocyte after 4 days of induction (IMP-4), subcutaneous preadipocyte (SCP-0), and subcutaneous adipocyte after 4 days of induction (SCP-4). We found 1419 and 697 differentially expressed genes and lncRNAs in the IMP-0-vs-IMP-4 group, respectively. There were 2307 and 1180 differentially expressed genes and differential lncRNAs, respectively, in the SCP-0-vs-SCP-4 group. The IMP-0-vs-IMP-4 and SCP-0-vs-SCP-4 groups were plotted using a venn diagram to obtain unique differential genes in each group, such as CHKA, PNPLA2, PLPP1, FABP4, ACSL5, UGT8, FAT1 and FADS2. In IMP-0-vs-IMP-4, significant enriched GO terms and KEGG pathways were regulation of MAPK cascade, lipid binding, Arachidonic acid metabolism, etc. In SCP-0-vs-SCP-4, beta-alanine metabolism, Wnt signaling pathway, lipid metabolic process and so on were most significant . Based on correlation analysis, the IMP-0-vs-IMP-4 group constructes a co-expression network of 193 nodes and 275 edges, while the SCP-0-vs-SCP-4 group constitutes a lncRNA-mRNA co-expression network with 564 nodes and 3,471 edges. Key lncRNAs, including MSTRG.8652.4, MSTRG.15586.1, MSTRG.6393.1, XR_217450.4, MSTRG.10341.22, XR_003492841.1, MSTRG.13937.5, and XR_003493886.1 were screened according to connectivity degree. Taken together, the current findings indicated that there are differentially regulated differential genes, lncRNAs, and enrichment pathways in IMP-0-vs-IMP-4 and SCP-0-vs-SCP-4. Because of being differentially regulated, some differential factors were significantly increased in expression in intramuscular adipocyte induction while significantly downregulated in subcutaneous adipocyte induction, such as FABP3, MSTRG.13937.5, and MSTRG.6393.1. Meanwhile, there were also some factors that were specifically regulated, CHKA, PLA2G4A, FADS2, MSTRG.13842.1, MSTRG.16051.2 and MSTRG.13842.1 were significantly downregulated only in subcutaneous adipocytes. This suggests that these lncRNAs and their target genes may play important roles in intramuscular fat and subcutaneous fat deposition.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic transcriptomic landscape of myogenesis in Muscovy ducks (Cairina moschata): Integrative analysis of hub genes post-hatching.","authors":"Xiaofeng Li, Bing Yang","doi":"10.5713/ab.25.0159","DOIUrl":"https://doi.org/10.5713/ab.25.0159","url":null,"abstract":"<p><strong>Objective‌: </strong>‌: Post-hatching myogenesis is a critical determinant of meat yield and quality, with potential regulatory roles of specific genes remaining underexplored‌ in Muscovy ducks (Cairina moschata). This study aimed to identify hub genes governing post-hatching myogenesis through transcriptomic profiling‌.</p><p><strong>Methods‌: </strong>‌: Three White-feathered male Muscovy ducks at 1-day-old (1D) and 80-day-old (80D) were selected, respectively. Following humane euthanasia, the left leg muscles were collected for subsequent RNA extraction, library construction, genome alignment, and transcriptome sequencing. DESeq2 (v1.16.1) was utilized to screen differentially expressed genes (DEGs) based on the criteria of |log2FoldChange| > 1 and P< 0.05 ‌‌. Functional enrichment (Gene Ontology, KEGG, PANTHER) and protein-protein interaction network analyses were conducted via DAVID, KOBAS, PANTHER, and STRING databases, with CytoHubba identifying myogenesis-associated hub genes‌.</p><p><strong>Results: </strong>‌: A total of 1,683 DEGs were identified between 80D and 1D muscles, including 865 upregulated and 818 downregulated genes‌. Totally ten hub genes, such as CD3E, ITK, COL4A6, HADHB, MCCC2, and IVD, were prioritized as key regulators of myogenesis‌. Functional analysis revealed their enrichment in L-leucine catabolic processes and fatty acid β-oxidation‌. Pathway mapping further associated these genes with folate-mediated one-carbon metabolism, glycine/serine/threonine metabolism, and branched-chain amino acid degradation‌.</p><p><strong>Conclusion: </strong>‌: This study delineates a molecular framework for post-hatching myogenesis in Muscovy ducks, highlighting ten hub genes that orchestrate myogenesis through metabolic reprogramming‌. These findings advance genetic strategies for improving poultry meat production and quality‌.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protection of bovine mammary epithelial cells against lipopolysaccharide-induced inflammatory responses using Centella asiatica through its antioxidant and anti-inflammatory activities.","authors":"Do Hyun Kim, Hyuk Cheol Kwon, Jong Hyeon Han, Hyun Su Jung, Dong-Min Shin, Sung Gu Han","doi":"10.5713/ab.25.0089","DOIUrl":"https://doi.org/10.5713/ab.25.0089","url":null,"abstract":"<p><strong>Background: </strong>: Bovine mastitis, an inflammatory condition affecting dairy cow udders, results in decreased milk quantity and quality, posing significant economic losses in the dairy industry. With increasing interest in natural products, Centella asiatica has garnered attention for its potent anti-inflammatory and antioxidant properties. Objective: This study aimed to investigate the potential protective effects of C. asiatica extracts (CE) against lipopolysaccharide (LPS)-induced inflammation in bovine mammary epithelial cells (MAC-T).</p><p><strong>Methods: </strong>: CEs were prepared by extracting C. asiatica leaf powder using ethanol at concentrations of 60%, 70%, 80%, 90%, and 100%. In LPS-stimulated MAC-T cells, the study investigated the ability of CE to reduce oxidative stress and inflammation and its impact on casein protein synthesis.</p><p><strong>Results: </strong>: C. asiatica extract with 60% ethanol (CE60) exhibited the highest radical scavenging activity and total phenolic content among the tested extracts. In MAC-T cells, CE60 significantly attenuated LPS-induced inflammation by down-regulating pro-inflammatory cytokines such as cyclooxygenase-2, tumor necrosis factor-α, interleukin (IL)-1β, and IL-6, as well as inhibiting nuclear factor kappa B activation. CE60 also reduced intracellular reactive oxygen species through upregulating nuclear factor erythroid-2-related factor 2 and associated antioxidant enzymes, including glutathione peroxidase (GPx) 1, GPx4, superoxide dismutase (SOD) 1, SOD2, and catalase. Moreover, CE60 restored the synthesis of casein proteins (CSN1S1, CSN1S2, and CSN2) in LPS-treated MAC-T cells, indicating a protective effect on lactation function under inflammatory conditions.</p><p><strong>Conclusion: </strong>: Taken together, CE60 has potential as a natural substance for the prevention of bovine mastitis by reducing oxidative stress and inflammation in mammary epithelial cells.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}