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IF 2.4 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Bioscience Pub Date : 2025-02-27 DOI:10.5713/ab.24.0816

Jian Wang, Xi Wu, Liuming Zhang, Xiaomei Sun, Wei Sun, Kunzhe Dong, Yongjun Li

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引用次数: 0

摘要

目的：毛发的形态发生与毛囊干细胞（HFSCs）的增殖和毛囊（HF）的发育密切相关。长江三角洲白山羊（YRDWG）的毛囊干细胞对生产优质刷毛（SQBH）非常重要。然而，已知的调控机制还不足以解释长三角白山羊高频间充质干细胞的生长、HF发育和SQBH的形成：为了深入研究circCOL1A1在YRDWG高频发育和SQBH形成过程中的相互作用网络和机制，我们对YRDWG的circCOL1A1-knockdown和circCOL1A1-overexpressing高频间充质干细胞进行了全转录组测序和生物信息学分析。利用 STRING 和其他数据库构建了多重相互作用网络。通过实时定量聚合酶链反应（RT-qPCR）和桑格测序进一步确认了差异表达（DE）基因、DE-miRNA和DE-circRNA：结果：在circCOL1A1-敲除和circCOL1A1-表达的gHFSCs之间，共有87个基因、96个miRNA和135个circRNA存在差异表达。通过功能富集、GO注释和KEGG分析发现，这些DE基因、DE-miRNA和DE-circRNA在MAPK、PI3K/Akt和局灶粘附信号通路中明显富集，而这些通路与gHFSCs的生长和HF的发育密切相关。此外，通过相互作用网络的构建，还得到了四个重要的调控轴，即chi-circCOL1A1-miR-149-5p-CMTM3-AR、chi-circACTN1-miR-671-5p-MAPK3/COL13A1、chi-circITGA6-miR-18a-5p-FGF1/MAP3K1和chi-circCOBLL1-miR-30a-5p/miR-128-3p-ITGA6/MAPK14/FGF14轴：这些新发现为研究circRNA参与和调控长三角白山羊高频发育和SQBH形成的复杂机制提供了有价值的综合依据。

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Integrative and comparative analysis of whole-transcriptome sequencing in circCOL1A1-knockdown and circCOL1A1-overexpressing goat hair follicle stem cells.

Objective: Hair morphogenesis is tightly related to hair follicle stem cells (HFSCs) proliferation and hair follicle (HF) development. Yangtze River Delta white goats (YRDWG) HFSCs are important for producing superior-quality brush hair (SQBH). Nonetheless, the known regulatory mechanisms are not sufficient to explain YRDWG gHFSCs growth, HF development, and SQBH formation.

Methods: To deeply investigate the interaction networks and mechanisms of circCOL1A1 in the HF development and SQBH formation of YRDWG in detail, we applied whole-transcriptome sequencing and bioinformatics analysis of circCOL1A1-knockdown and circCOL1A1-overexpressing HFSCs from YRDWG. STRING and other databases were used to construct multiple interaction networks. Differentially expressed (DE) genes, DE-miRNAs, and DE-circRNAs were further confirmed via real-time quantitative polymerase chain reaction (RT-qPCR) and Sanger sequencing.

Results: A total of 87 genes, 96 miRNAs, and 135 circRNAs were differentially expressed between circCOL1A1-knockdown and circCOL1A1-overexpressing gHFSCs. Functional enrichment, GO annotation and KEGG analyses identified marked enrichment of these DE-genes, DE-miRNAs, and DE-circRNAs in the MAPK, PI3K/Akt, and focal adhesion signaling pathways, which are closely associated with gHFSCs growth and HF development. In addition, through interaction network construction, four important regulatory axes were obtained, namely, the chi-circCOL1A1-miR-149-5p-CMTM3-AR, chi-circACTN1-miR-671-5p-MAPK3/COL13A1, chi-circITGA6-miR-18a-5p-FGF1/MAP3K1 and chi-circCOBLL1-miR-30a-5p/miR-128-3p-ITGA6/MAPK14/FGF14 axes.

Conclusion: These novel findings provide a valuable and comprehensive basis for investigating the complex mechanism by which circRNAs participate in and regulate HF development and SQBH formation in Yangtze River Delta white goats.

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