Animal Bioscience最新文献

{"title":"Population structure and genome-wide association study of body conformation traits of two native goat breeds in China.","authors":"Rong Yang, Di Zhou, Yanli Lv, Xingzhou Tian, Liqun Ren, Fu Wang, Zhengang Guo, Yongju Zhao, Jipan Zhang","doi":"10.5713/ab.25.0334","DOIUrl":"https://doi.org/10.5713/ab.25.0334","url":null,"abstract":"<p><strong>Objective: </strong>Body conformation traits have a direct impact on carcass performance in the meat goat industry. This study explored the population genetics of two Chinese goat breeds and identified the genomic variates contributing to their body conformation traits.</p><p><strong>Methods: </strong>The Guizhou black goat (GBG, n=104) and Hezhang black goat (HBG, n=100) were genotyped by whole-genome sequencing and phenotyped by measuring their body height (BH), body length (BL), chest depth (CD), chest width (CW), chest girth (CG), rump width (RW), rump height (RH), and cannon circumference (CC).</p><p><strong>Results: </strong>The relatedness analysis showed that these goats were low genetically kinship-related, with the GBG and HBG being relatively independent but with some genetic introgression. The lambda values showed that the GWAS model was reliable, and a total of 33, 1, 6, 2, 5, 10, 21, and 13 SNPs were identified as significantly correlated (P<8.33e-8) with BH, BL, CD, CW, CG, RW, RH, and CC, respectively. Interestingly, the GWAS for BH and RH identified the greatest number of significant SNPs, with a large proportion of these SNPs overlapping and being mainly located in four regions: chr13_63286230-69784740 (10 SNPs), chr14_60354209-60376549 (six SNPs), and chr15_65605417-73873841 (five SNPs), and chr23_42819635-43332716 (nine SNPs). Individuals carrying more of these SNPs had higher BH and RH values. After annotation for all the significant SNPs, 103 genes located in a ±100 Kb region were identified, where the most significantly enriched KEGG pathway was \"Olfactory transduction\", and the most significantly enriched GO terms were \"cellular process\" and \"molecular transducer activity\".</p><p><strong>Conclusion: </strong>This study investigated the population genetics of two prominent Chinese goat breeds and identified several SNPs that are significantly associated with body conformation traits. These findings provide biological insights on enhancing growth performance and have great potential for practical application in the genomic selection of meat goats.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of a calcium soap and jackfruit leaf extract on blood metabolites, biomarkers of oxidative stress and follicle growth of crossbreed doelings.","authors":"Yusti Pujiawati, I Komang Gede Wiryawan, Lilis Khotijah, Muhammad Agus Setiadi, Simon Petrus Ginting","doi":"10.5713/ab.25.0337","DOIUrl":"https://doi.org/10.5713/ab.25.0337","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluates the effect of a flushing ration containing polyunsaturated fatty acids (PUFA) and jackfruit leaf extracts (JLE) on the follicle growth, reproductive hormone profiles and oxidative stress state of doelings.</p><p><strong>Method: </strong>Fifteen primiparous Boer x Jawarandu doelings (23.21±3.57, 10-11 months old) were divided into three groups: control (PUFA flushing ration), control+JLE (200 mg/kg DMI), and control+Ca-soap (calcium soap)+JLE (200 mg/kg DMI). The researchers measured the following parameters production performances, blood metabolites, antioxidant status, estradiol, progesterone, and follicle development.</p><p><strong>Results: </strong>The doelings' glucose levels showed significant differences (p<0.05) during the post-flushing (PF) phase, on days 19, and day 21 after estrus. An increase in glucose level was observed following the administration of the control+Ca-soap+JLE ration. However, on day 21 after estrus, the highest glucose level was detected in the control+JLE treatment group (p<0.05). In addition, the doelings' cholesterol levels were elevated on days 19 and 21 with the administration of the control+JLE ration. These changes in glucose and cholesterol levels between phases are presumed to be associated with the increased demand for hormone synthesis and follicular development. JLE supplementation reduced malondialdehyde (MDA) levels across all phases (p<0.05), indicating lower oxidative stress. Moreover the administration of the control+JLE flushing ration resulted in the highest estradiol hormone level on day 19 after estrus compared to other treatments.This finding is consistent with the greater number of medium-sized follicles observed in doelings receiving the same treatment. Additionally,including JLE in the flushing ration also increased the doelings' progesterone hormone levels during the luteal phase.</p><p><strong>Conclusion: </strong>Jackfruit leaf extract can mitigate oxidative stress by reducing blood MDA levels during the pre-mating phase. The combination of a polyunsaturated fatty acid flushing ration with JLE may enhance follicular development, particularly medium and large-sized follicles.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of fermented rice bran extract with multi-microbial species on intestinal health and growth of nursery pigs.","authors":"Lan Zheng, Jeonghyeon Son, Sung Woo Kim","doi":"10.5713/ab.25.0402","DOIUrl":"https://doi.org/10.5713/ab.25.0402","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to investigate the effects of fermented rice bran extract (FRB) with multi-microbial species on intestinal health and growth performance of nursery pigs.</p><p><strong>Methods: </strong>Thirty weaned pigs (initial body weight = 6.8±0.8 kg) were allocated to 3 dietary treatments in a randomized complete block design (n = 10 per treatment) and fed for 25 d in 2 phases (7 and 18 d, respectively). Pigs were housed individually in pens equipped with a feeder and a nipple drinker. Pigs were fed a basal diet supplemented with 0%, 0.5%, or 1.0% FRB. The main feedstuffs of the basal diet were corn, soybean meal, whey permeate, and blood plasma. The FRB was prepared by fermenting rice bran with Lactobacillus plantarum, Bacillus subtilis, and Saccharomyces cerevisiae, and contained at least 1×107 CFU/g. On d 25, pigs were euthanized to collect intestinal tissues and mucosa for intestinal health.</p><p><strong>Results: </strong>The supplementation of FRB decreased (p<0.05) the abundance of Desulfovibrio piger in the jejunal mucosa. Malondialdehyde and protein carbonyl in the duodenum linearly decreased (p<0.05) and the villus height to crypt depth ratio in the jejunum linearly increased (p<0.05) with increasing FRB supplementation. The apparent ileal digestibility of gross energy and crude protein tended to linearly increase (p=0.084 and p=0.098, respectively). Body weight on d 25 and overall average daily gain tended to show quadratic responses (p=0.084 and p=0.095, respectively) with increasing FRB supplementation. The gain to feed ratio (G:F) was maximized when the FRB intake was 2.7 g/d (0.48%) during d 7 to 25.</p><p><strong>Conclusion: </strong>Dietary supplementation with FRB with multi-microbial species improved intestinal health based on immune response, oxidative stress, and morphology. The growth performance of nursery pigs showed quadratic responses with increasing FRB supplementation. Specifically, the G:F was maximized with supplementation of FRB at 0.48%.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mechanism of m6A methylation analysis of the transcriptome to regulate the diameter of Alpine Merino wool fiber.","authors":"Lin Yue, Ting Ting Guo, Bo Wen Chen, Jian Bin Liu, Zeng Kui Lu, Chao Yuan","doi":"10.5713/ab.25.0347","DOIUrl":"https://doi.org/10.5713/ab.25.0347","url":null,"abstract":"<p><strong>Objective: </strong>Wool is an important textile raw material, and fiber diameter is a major determinant of the economic value and quality of wool products. Analyses of the regulatory mechanisms underlying wool fiber diameter are necessary for the development of strategies to improve wool fineness. Therefore, we used methylationomics to analyze the skin tissue of individuals with different fiber diameters, and analyzed the apparent regulation mechanism of wool fiber diameter.</p><p><strong>Methods: </strong>In this study, we jointly analyzed the transcriptome and m6A methylome of skin tissues from individual Alpine Merino sheep with different wool fiber diameters (classified into three groups) to mine key methylated RNAs and explore the significance of m6A methylation in the regulation of this trait.</p><p><strong>Result: </strong>In total, 54,057 methylated peaks, 4,273 differentially methylated genes, 139 differentially methylated lncRNAs, and 2,992 differentially methylated circRNAs were found in the three comparisons. These loci were enriched in the Wnt, Notch, and TGF-β signaling pathways, as determined through GO and KEGG pathway analyses. RNA correlation analyses revealed key RNAs, such as CACNA1E, FOS, CAMK2B, RNF43, circ-0317, circ-4794, TCONS-00020832, and TCONS-00020845, indicating that hypermethylation may be an important factor affecting wool fiber diameter.</p><p><strong>Conclusion: </strong>These findings provide insight into the molecular regulatory mechanism underlying wool fiber diameter and provide a theoretical basis for the development of the wool industry.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Weaning Stress and Rumen Microbiota in Goat Kids: Evidence from Granger Causality and Randomized Controlled Trial Validation.","authors":"Ziwei Peng, Hanjie Xiao, Weiwei Yang, Tong Wang, Haojiong Xie, Hui Yan, Shoukun Ji","doi":"10.5713/ab.25.0092","DOIUrl":"https://doi.org/10.5713/ab.25.0092","url":null,"abstract":"<p><strong>Objective: </strong>Weaning stress is frequently associated with disrupted gastrointestinal microbiota and decreased growth performance, the association between them remains unclear.</p><p><strong>Methods: </strong>In this study, we employed Granger causality inference to compare the dynamic changes in gastrointestinal microbiota between stressed and non-stressed goat kids, followed by validation through a randomized controlled trial involving rumen microbiota transplantation.</p><p><strong>Results: </strong>Our findings indicated that the alpha diversity and microbial maturity of the rumen microbiota in stressed kids were significantly lower than those in non-stressed kids during the weaning period (p<0.05). Furthermore, the rumen microbiota at pre-weaning could accurately predict the body weight changes during weaning stress period (R2=0.99, RMSPE=0.19). We demonstrated that the cultured rumen microbiota retained most high-abundance rumen bacteria (89.22%), and its transplantation effectively altered the rumen microbiota (ANOSIM, p<0.05), enhanced microbial maturity (p<0.05), and improved growth performance (p<0.05) during weaning, without affecting the gut microbiota (p>0.05). Additionally, transplantation of the cultured rumen microbiota reduced intestinal permeability and inflammation while increasing antioxidant levels in weaned kids(p<0.05).</p><p><strong>Conclusion: </strong>These findings provide evidence for the association between rumen microbiota and weaning stress, demonstrating that manipulating rumen microbiota is an effective strategy for alleviating weaning stress in goat kids.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Light Duration and Variation on the Growth and Reproductive Performance in Breeder Geese.","authors":"Min Jung Lin, Shen Chang Chang, Li Jen Lin, Jhih Siang Chang, Shao Yu Peng, Tzu-Tai Lee","doi":"10.5713/ab.25.0349","DOIUrl":"https://doi.org/10.5713/ab.25.0349","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to investigate the effects of time and light variation on the growth and reproductive performances of breeder geese.</p><p><strong>Methods: </strong>A total of 32 ganders and 96 dames in their first laying season, with an average age of 10 months, were assigned to eight rooms, each containing four ganders and twelve dams. A split-plot design was used, incorporating two prelay photoperiod treatments: a 7-hour light period (P7H), in which the photoperiod was maintained at seven hours for the first six weeks, and a gradually decreasing light (GDL) group, in which the light duration was initially set at 12 hours and then reduced by one hour per week during the prelay period. Two variable photoperiod schedules were applied as sub-treatments and randomized across the rooms. In one treatment, the egg production rate in breeder geese decreased to an average below 30%, after which the photoperiod increased by 15 minutes each week (change time, CHP). A fixed photoperiod of nine hours was maintained (fixed time, FIXP) until the end of the egg-laying period.</p><p><strong>Results: </strong>Geese in the GDL light group had a longer laying duration than P7H light group (243.75 vs 191.75 days; p<0.01). Number of eggs per goose in the GDL light group showed a trend toward higher values than P7H light group. However, the fertility and hatchability in GDL light group were significantly lower than the P7H light group at all periods, respectively.</p><p><strong>Conclusion: </strong>Geese exposed to a 12-hour light (12L:12D) regimen during the pre-laying period exhibited a longer laying duration and higher egg production per bird. However, maintaining a fixed lighting schedule of 9 hours of light and 15 hours of darkness (9L:15D) after the peak laying period is recommended to optimize production profitability by supporting better fertility and hatchability.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions between extracellular vesicles and hormones.","authors":"Yiwen Bi, Yang Yang, Linsheng Gan, Wenhui Huang, Xin Feng, Huihua Zhang, Jie Liu, Limin Wei, Qien Qi","doi":"10.5713/ab.25.0249","DOIUrl":"https://doi.org/10.5713/ab.25.0249","url":null,"abstract":"<p><p>This review explores the interaction between extracellular vesicles (EVs) and hormones, highlighting their importance in physiological and pathological conditions. As key messengers of intercellular communication, extracellular vesicles are rich in a variety of bioactive molecules and can play an important role in the in vivo environment. Hormones, as the core mediators of regulating the functions of organisms, affect the production and release of extracellular vesicles through a variety of signaling pathways, and then regulate the function of target cells. Conversely, extracellular vesicles can also carry biologically active molecules that significantly affect hormone synthesis and secretion. In this review, the effects of different hormones (such as estrogen, testosterone, follicle-stimulating hormone, etc.) on extracellular vesicles and the regulatory effects of extracellular vesicles on hormone secretion are discussed in detail, which reveals the potential clinical application value of this field, including the possibility of being a biomarker and a new direction of personalized medicine. Future studies are needed to further understand the molecular mechanism of this interaction to promote the development of new diagnostic tools and therapeutic strategies.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semirefined glycerin as an alternative energy source on intake, digestibility and milk production of lactating cows.","authors":"Murilo de Almeida Meneses, Robério Rodrigues Silva, Dicastro Dias de Souza, Eli Santana de Oliveira Rodrigues, Wendel Bispo Santos E Santos, Lara Maria Santos Brant, José Esler Freitas Júnior, Fabiano Ferreira da Silva","doi":"10.5713/ab.25.0319","DOIUrl":"https://doi.org/10.5713/ab.25.0319","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the effect of dietary glycerin levels on feed intake, nutrient digestibility, milk yield and composition, nitrogen balance, microbial production, and eating behavior in confined lactating cows.</p><p><strong>Methods: </strong>Eight crossbred cows (Holstein × Zebu) were used, with an average of 90 ± 10 days in milk, milk production of 21.3 ± 0.47 kg/day, and body weight of 570 ± 51 kg. The cows were assigned to two 4×4 Latin squares with the following treatments: Control (G0), no glycerin inclusion; G6, 6% glycerin inclusion in the total dietary dry matter (DM); G12, 12% glycerin inclusion; and G18, 18% glycerin inclusion.</p><p><strong>Results: </strong>Glycerin inclusion linearly decreased DM intake (p<0.001), crude protein (CP) intake (p=0.004), and neutral detergent fiber (NDF) intake (p<0.001). On the other hand, the digestibility coefficients of DM (p=0.001), CP (p=0.007), non-fiber carbohydrates (NFC) (p<0.001), and total digestible nutrients (TDN) (p=0.013) increased linearly. Milk yield (P=0.046) and 3.5% fat-corrected milk (FCM) (p=0.013) decreased linearly.</p><p><strong>Conclusion: </strong>Despite increasing the digestibility of nutrients, the inclusion of glycerin in diets for feedlot dairy cows resulted in a significant reduction in dry matter intake, which compromised total energy intake and, consequently, milk production. Thus, although it is a promising co-product due to its availability and energy value, semi-refined glycerin, in the proportions tested, was not viable as an energy substitute in diets of lactating cows, requiring caution not to compromise productive performance.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Replacement of soya DOC with rice DDGS and its effect on performance, nutrient metabolizability, carcass traits and gut health of broiler chicken.","authors":"Atul Parashuram Dhok, Roshan Lavakumar Werulakar, Sudhir Bhimrao Kawitkar, Nitin Vasantrao Kurkure, Pratiksh Kaluram Bacche, Mahesh Ravindra Jawale, Shital Vitthalrao Chopde, Roupesh G Roupessss, Darshana B Bhaisare, Madhuri S Hedaoo, Shilpa V Shinde, Shweta Ramesh Lende","doi":"10.5713/ab.23.0533","DOIUrl":"https://doi.org/10.5713/ab.23.0533","url":null,"abstract":"<p><strong>Objective: </strong>- The study was conducted to utilize rice DDGS as a protein source in broiler chicken.</p><p><strong>Methods: </strong>- Three hundred day old straight run commercial broiler chicks were divided into five groups of four replications each and fed as CG control receiving standard basal diet; 15-RDDGS with 15 percent rice DDGS; 15-RDDGSE with 15 percent rice DDGS supplemented with protease enzyme @ 300g/ton of feed; 20-RDDGS with 20 percent rice DDGS and 20-RDDGSE with 20 percent rice DDGS supplemented with protease enzyme @ 300g/ton of feed.</p><p><strong>Results: </strong>- The final body weights were significantly (p<0.01) higher and FCR was significantly better for CG and comparable with 15-RDDGS and 15-RDDGSE. The CP metabolizability, was significantly (p<0.05) higher for CG than 15 and 20 percent rice DDGS groups, however the CP metabolizability for protease supplemented 15 and 20 percent rice DDGS group was comparable with control diet. The total viable count (TVC) and E-coli count revealed non-significant differences. There was no negative impact on histological changes in intestine due to inclusion of rice DDGS. There was no negative impact on histological changes such as crypt depth, villus width and villus height in intestine due to interaction between DDGS and enzyme. The carcass traits revealed non-significant differences for dressing percentage, overall giblet weight and abdominal fat.</p><p><strong>Conclusion: </strong>- It was concluded that rice DDGS can be incorporated upto 20 levels in broiler diet without any adverse effect, however inclusion of 15 percent rice DDGS supplemented with protease enzyme proved to be more economical in broilers.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of injured muscle identifies new candidate genes for satellite cell growth and myofiber formation during early muscle regeneration.","authors":"Zhuning Yuan, Xian Tong, Xianyao Luo, Liping Pan, Hoi-Ka Wu, Rong Xu, Ziyun Liang, Xunhe Huang, Delin Mo","doi":"10.5713/ab.24.0859","DOIUrl":"https://doi.org/10.5713/ab.24.0859","url":null,"abstract":"<p><strong>Objective: </strong>: The self-repair capacity of skeletal muscle arouses interest in studying satellite cell activity and myofiber formation. The major molecular networks of satellite cell activity have been extensively studied. However, the mechanism by which micro-environmental factors regulate satellite cell activity for early muscle regeneration still remains poorly understood.</p><p><strong>Methods: </strong>: Hematoxylin and eosin (HE) staining and immunofluorescence for embryonic myosin heavy chain (eMyHC) were performed on control and injured muscle samples at 12-, 24-, 36-, 48-, 60-, 72-, and 84-hour post-injury. Additionally, eMyHC immunofluorescence was conducted on muscle samples collected 96 hours post-injury from three mice. RNA sequencing (RNA-seq) and quantitative PCR (qPCR) were performed on samples from 24 mice, including controls and samples at 12-, 24-, and 84-hour post-injury.</p><p><strong>Results: </strong>: In this research, 516 immune-related and 177 hormone response-related genes were up-regulated significantly. Further, statistical analysis indicated that the number of differentially expressed genes (DEGs) between up- and down-regulated immune system-related DEGs was similar with that of hormone response-related DEGs. Notably, p53 signaling pathway was significantly enriched throughout early muscle regeneration. Based on patterns of crucial myogenic genes expression, 326 and 320 candidate genes related to satellite cell growth and myofiber formation were obtained, respectively. Furthermore, through interaction network analysis, 41 immune factors, including S100a9，Csf3r，Cxcl3，Ppbp，Ccl3，Il1rn were found, which may regulate satellite cell activation, migration and proliferation. Likewise, 16 cell adhesion factors (Col1a2, Cdh2, Thbs2, etc.) may be involved in myofiber formation.</p><p><strong>Conclusion: </strong>: This study leveraged transcriptomic analysis to uncover key candidate genes and biological processes involved in early muscle regeneration. These findings enhance our understanding of the molecular mechanisms underlying muscle repair and offer insights for future therapeutic strategies.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}