Animal Bioscience最新文献

{"title":"Putative proteomic signatures associated with post-thaw semen quality in Peranakan Etawah and Saanen goats: an exploratory study.","authors":"Muhammad Ihsan Andi Dagong, Muhammad Yusuf, Athhar Manabi Diansyah, Procula Rudlof Matitaputty, Paskah Partogi Agung, Indra Wahyudi Syarif, Muhammad Fajar Amrullah","doi":"10.5713/ab.260268","DOIUrl":"https://doi.org/10.5713/ab.260268","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to compare post-thaw semen quality and sperm kinematics between Peranakan Etawah (PE) and Saanen goats and to identify breed-associated proteomic signatures related to semen cryotolerance.</p><p><strong>Methods: </strong>Frozen semen straws were obtained from BIB Singosari, Indonesia, and grouped by breed. Post-thaw evaluations included viability, morphology/abnormality, plasma membrane integrity (HOST), and computer-assisted sperm analysis for motility and kinematic parameters. Sperm proteomes were profiled using UHPLC-Orbitrap LC-MS/MS. Data were processed in Proteome Discoverer and analyzed using multivariate models (PCA, PLS-DA), differential abundance testing (q-values), functional enrichment (g:Profiler), biomarker selection (VIP > 1; AUC ≥ 0.70), and correlation with semen traits (MetaboAnalyst).</p><p><strong>Results: </strong>PE and Saanen differed significantly in survival-related traits, including viability, abnormality, and plasma membrane integrity (p < 0.05), while total motility and all kinematic parameters were similar between breeds (p > 0.05). Proteomic patterns showed partial separation by PCA and clearer discrimination by validated PLS-DA. Multiple proteins were differentially abundant between breeds (q < 0.05) and clustered consistently by breed. Enrichment analysis highlighted mitochondrion-associated functions, particularly fatty acid β-oxidation. Candidate discriminative proteins included ACADVL, ETFB, GPD2, CYLC1, and LOC102188264, which were significantly associated with semen quality traits within breeds.</p><p><strong>Conclusion: </strong>Breed-related differences in frozen-thawed goat semen were mainly reflected in post-thaw survival traits and in proteomic signatures dominated by mitochondrial energy metabolism. Integrating proteomics with conventional semen assessment may help explain semen cryotolerance and support biomarker discovery.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of heat stress on physiological, endocrine, and reproductive functions in swine: a review.","authors":"Jae-Hyeok Lee, Se-Na Jo, Jin-Su Kim, Jae-Cheol Jang","doi":"10.5713/ab.250866","DOIUrl":"https://doi.org/10.5713/ab.250866","url":null,"abstract":"<p><p>Climate change driven heat stress (HS) causes major losses in the productive and reproductive performance of swine. Using temperature-humidity index (THI) based quantification of heat load as a framework, this review integrates HS effects across the endocrine and metabolic axis, the gut barrier microbiota, immune axis, and the reproductive axis. Heat stress, in the context of hypothalamic, pituitary, adrenal (HPA) sympathetic-adrenomedullary activation and insulin, catecholamine, and cortisol crosstalk, is accompanied by relative hyperinsulinemia and, via pyruvate dehydrogenase kinase 4 mediated pyruvate dehydrogenase inhibition and the lactate dehydrogenase/hypoxia-inducible factor-1α (HIF-1α) axis, repartitions substrate utilization toward glucose, thereby constraining lipid mobilization. Within the gut immune axis, β-diversity, centered dysbiosis and short chain fatty acids (SCFA) depletion weaken HIF-1α, dependent mucosal barrier defenses; together with increased lipopolysaccharide translocation, this hyperactivates TLR4/ NF-κB signaling, the NLR family pyrin domain containing 3 inflammasome, and Th17/IL-17 pathways. Evidence from fecal microbiota transplantation supports a causal role for dysbiosis in pathogenesis. In the reproductive axis, dysregulated hypothalamic-pituitary-gonadal-HPA crosstalk impairs follicular and luteal function and compromises placental nutrient transport; intrauterine HS can reprogram F1 offspring, with lasting effects on metabolism, immunity, and reproduction. In conclusion, HS is a systems level stressor that perturbs multiple physiological axes; progress will require standardized HS models, multi omics and multigenerational follow up, and integrated environment, nutrition, and management strategies. Beta diversity shifts in the gut microbiota and SCFA depletion emerge as core pathophysiologic mediators, supporting their use as early biomarkers and intervention targets and underscoring the need for focused investigation.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating selective signature analysis and genome-wide association study to identify candidate genes associated with litter size in Leizhou goat （A revised version）.","authors":"Da Feng, Siyi Hu, Jian Ma, Chunmei Du, Guangxian Zhou, Chen Wei, Shangquan Gan","doi":"10.5713/ab.260280","DOIUrl":"https://doi.org/10.5713/ab.260280","url":null,"abstract":"<p><strong>Objective: </strong>Litter size is a key trait for evaluating reproductive performance of goats, which can directly influence the breeding efficiency and profitability of production cycles. However, a comprehensive understanding of its genetic architecture, including the causative mutations and their functional impacts, remains scarce, thus hindering its application in precision breed-ing.</p><p><strong>Methods: </strong>A total of 92 female Leizhou goats were genotyped through whole-genome se-quencing (WGS), and their litter size were measured for three consecutive births. Subsequently, based on the genotype and phenotype data, population genetic structure analysis, selection signature analysis, and genome-wide association study (GWAS) were performed.</p><p><strong>Results: </strong>We identified a total of 20,802,514 SNPs in the 92 Leizhou goats. Analysis of genetic structure revealed that the high-litter-size group exhibited greater genetic diversity than the low-litter-size group, although no significant genetic differentiation was detected between them. We identified 340 putative selective regions and 346 candidate genes across the genome. A total of 37 candidate genes were identified as being closely associated with reproductive traits, including BMP6, MAPK8, RPTOR, etc. In GWAS results, we identified 63 quantitative trait loci (QTLs) for litter size at suggestive significance (P＜1×10-5). After, 207 candidate genes were annotated, and 4 of them were highlighted that have been previously reported to be associated with reproductive processes (e.g., TMEM100, PDGFD, NLRP9, and ARHGAP22). By integrating the results from both approaches, we identified MAPK8 as a potential candidate gene associated with litter size. Furthermore, our findings from motif recognition, transcrip-tion factor prediction, and previous studies lead us to propose that the BMP6-GATA4-MAPK8 signaling pathway may play a key role in regulating litter size in goats.</p><p><strong>Conclusion: </strong>Our findings identify several candidate genes and putative associated variants for litter size, providing valuable insights into the genetic basis of reproductive traits in goats.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-27b-3p aggravates steatohepatitis progression by targeting the Nrf2/HO-1 pathway.","authors":"Kui Wang, Hao Pan, Yan Tian, Kang Feng Jiang, Xiao Bing Li","doi":"10.5713/ab.260476","DOIUrl":"https://doi.org/10.5713/ab.260476","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate whether miR-27b-3p regulates oxidative stress and inflammatory responses through the Nrf2/HO-1 signaling pathway during the progression of fatty liver.</p><p><strong>Methods: </strong>Liver tissues from dairy cows with fatty liver and high-fat diet (HFD)-induced fatty liver mice were collected to evaluate lipid accumulation, oxidative stress, inflammation, and the expression of miR-27b-3p and Nrf2/HO-1 pathway components. An in vitro steatosis model was established in AML-12 hepatocytes using palmitic acid (PA). Oxidative stress markers (ROS, SOD, and MDA), inflammatory cytokines (IL-1β, IL-6, and TNF-α), and apoptosis-related proteins were assessed. Bioinformatic prediction and dual-luciferase reporter assays were used to determine the targeting relationship between miR-27b-3p and Nrf2. Gain- and loss-of-function experiments were performed using miR-27b-3p mimics and inhibitors to evaluate its regulatory effects on the Nrf2/HO-1 pathway and downstream cellular responses.</p><p><strong>Results: </strong>Fatty liver tissues from dairy cows and HFD-fed mice exhibited significant lipid deposition, enhanced oxidative stress, and elevated inflammatory responses, accompanied by increased miR-27b-3p expression and reduced Nrf2/HO-1 signaling activity. Similar alterations were observed in PA-treated AML-12 cells. Bioinformatic analysis and dual-luciferase assays confirmed that miR-27b-3p directly binds to the 3' untranslated region of Nrf2. Inhibition of miR-27b-3p restored Nrf2 and HO-1 expression, reduced ROS and MDA levels, increased SOD activity, suppressed NF-κB activation, decreased pro-inflammatory cytokine production, and alleviated apoptosis. Conversely, overexpression of miR-27b-3p further inhibited Nrf2/HO-1 signaling and exacerbated oxidative stress, inflammation, and cell injury.</p><p><strong>Conclusion: </strong>miR-27b-3p promotes the progression of fatty liver toward steatohepatitis by directly targeting Nrf2 and suppressing the Nrf2/HO-1 antioxidant pathway, thereby enhancing oxidative stress, inflammatory responses, and hepatocyte apoptosis. These findings identify miR-27b-3p as a key upstream regulator of redox imbalance in fatty liver and suggest that targeting miR-27b-3p may represent a promising strategy for the prevention and treatment of bovine fatty liver disease.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of psyllium husk (Plantago ovata) on the physicochemical and textural properties of chicken sausages and its application in an in vitro digestion model.","authors":"Ji Seon Choi, Koo Bok Chin","doi":"10.5713/ab.260344","DOIUrl":"https://doi.org/10.5713/ab.260344","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the quality characteristics of chicken sausages according to the amount of added psyllium husk powder (PHP; 0, 0.5, 1.0, and 1.5%) and determine protein digestibility using an in vitro digestion model.</p><p><strong>Methods: </strong>The effect of PHP on the quality of chicken sausages was assessed in terms of physicochemical properties and protein digestibility.</p><p><strong>Results: </strong>As the PHP levels increased, lightness decreased and yellowness increased in a concentration-dependent manner (p < 0.05). An increase in PHP concentration was also associated with a reduction in cooking loss and expressible moisture (p < 0.05), demonstrating that PHP possesses water-holding capacity (WHC). No textural property differences were observed among the treatments (p > 0.05). Furthermore, sausages containing 1.0‒1.5% PHP exhibited improved protein digestibility during in vitro digestion (p < 0.05). These findings indicated that PHP enhanced certain quality attributes in sausages, including WHC and digestibility, without negatively affecting texture. PHP addition showed negative correlations with CL, lightness, EM, and springiness, and positive correlations with yellowness and protein digestibility.</p><p><strong>Conclusion: </strong>Therefore, this study suggests that PHP can be used as a novel functional ingredient in meat products and may positively impact in vitro protein digestibility.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant activity of hot-air-dried sweet pumpkin's flesh and peel and their effects on the shelf-life and quality of pork emulsion-type model sausages during storage.","authors":"Cha Bin Park, Koo Bok Chin","doi":"10.5713/ab.260307","DOIUrl":"https://doi.org/10.5713/ab.260307","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the antioxidant activities of sweet pumpkin (Cucurbita maxima) flesh powder (FP) and peel powder (PP) and their effects on physicochemical and quality traits of emulsion-type model sausages during refrigerated storage.</p><p><strong>Methods: </strong>The antioxidant activity of FP and PP, which were dried by oven, was evaluated, and a total of 4 sausage groups including control, reference (ascorbic acid) and 2 treatments (FP and PP) were manufactured, and physicochemical and textural properties were measured during refrigerated storage. pH, color, cooking loss, expressible moisture, texture properties, thiobarbituric acid reactive substances (TBARS), volatile basic nitrogen (VBN), and microbial counts were conducted.</p><p><strong>Results: </strong>The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was higher in FP than PP at all concentrations except 1.0% (p<0.05), with 0.25% FP comparable to REF (p>0.05). Reducing power was higher in FP at 1.0% than in PP at the same concentration (p<0.05). Both the FP- and PP- treated emulsion-type model sausages showed reduced L* values; however, a* was higher in the FP-treated sausages, but lower in PP-treated sausages. Expressible moisture and TBARS values were also lower in treated sausages than the control (p<0.05).</p><p><strong>Conclusion: </strong>Sweet pumpkin both FP and PP were effective natural antioxidants with improved water holding capacity of sausages during storage.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and characterization of Hanwoo cumulus cell line for heat stress studies.","authors":"Ismail Shaleh, Sung Woo Kim, Tae Sub Park, Joonghoon Park","doi":"10.5713/ab.250896","DOIUrl":"10.5713/ab.250896","url":null,"abstract":"<p><strong>Objective: </strong>Heat stress (HS) negatively affects cattle reproduction, decreasing livestock productivity. HS response in cattle reproductive cumulus cells remain understudied due to limited cell lines and comprehensive omics data. This study aims to establish immortalized cumulus cells from Hanwoo cattle as a HS model and explores their HS response using molecular markers including transcriptomics and proteomics approaches.</p><p><strong>Methods: </strong>Immortalized cumulus cells were established from primary cumulus isolated from fresh Hanwoo cattle follicles by using piggyBac transposon-mediated SV40T expression system. HS condition was optimized using reactive oxygen species (ROS) detection, quantitative reverse-transcription polymerase chain reaction, and Western blotting on oxidative and endoplasmic reticulum stress markers under different HS and recovery periods (4 h, 12 h, and 24 h). Transcriptomic and proteomic data were generated to identify HS-related differentially expressed genes (DEG) and proteins (DEP) utilized for gene ontology (GO) and pathway enrichment analysis.</p><p><strong>Results: </strong>We established the first immortalized cumulus cell line with high specificity (99% CD44[+]) for in vitro HS study. ROS accumulation was observed at 4 h HS, leading to immediate upregulation of HSP70 and BiP proteins, with oxidative stress gene expression (SOD1, CAT, GPX1) peaked at 12 h HS. We identified 754 DEGs (fold change≥|2|) and 357 DEPs (fold change≥|1.5|) related to HS in the cumulus cell line. Enrichment analysis exhibited upregulation in GO and pathways related to protein unfolding and cellular response to HS, respectively, from both the DEG and DEP list. Downregulated DEG and DEP showed significant enrichment in GO related to the extracellular matrix, potentially affecting cumulus-oocyte complex communication for reproductive function.</p><p><strong>Conclusion: </strong>The cumulus cell line serves as a robust in vitro model for studying HS response in cattle reproductive system. The multi-omics data could help clarify the comprehensive HS response and identify possible molecular targets to mitigate negative HS effects in the future.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"250896"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148262852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association and Mendelian randomization analyses of placental efficiency and piglet birth weight in Danish Large White pigs.","authors":"Shi Xin Yu, Sheng Di Cui, Dong Chen, Zhen Jian Zhao, Patrick Kofi Makafui Tecku, Jia Miao Chen, Run Jie Huang, Yao Xi Zhou, Guo Qing Tang","doi":"10.5713/ab.250992","DOIUrl":"10.5713/ab.250992","url":null,"abstract":"<p><strong>Objective: </strong>Modern swine breeding has been shifted from maximizing productivity to improving quality for better efficiency. A key goal is to balance litter size with piglet birth weight (BW). This study focused on Danish Large White pigs to evaluate a novel placental efficiency (PEA) index based on placental area (PA) as a biologically relevant and measurable trait for breeding programs.</p><p><strong>Methods: </strong>The study integrated genome-wide association studies and Mendelian randomization to explore the genetic relationship and potential causal direction between the proposed PEA index and piglet BW. The investigation used data from 113 piglets.</p><p><strong>Results: </strong>Analysis revealed a significant positive causal effect of PEA on BW. Nine significant single nucleotide polymorphisms and eight candidate genes (MARVELD3, CMTR2, TLE7, CHST4, TAT, ZNF23, CALB2, PHLPP2) were identified. Functional enrichment analysis indicated that these genes synergistically regulate placental function: TAT is involved in amino acid metabolism and energy homeostasis; CALB2 may influence trophoblast signaling and placental vascular function via calcium regulation; CHST4 participates in extracellular matrix modification at the maternal-fetal interface; MARVELD3 contributes to placental barrier maintenance; CMTR2 and TLE7 are involved in RNA modification and transcriptional regulation; ZNF23 may regulate cell cycle and differentiation; and PHLPP2 influences cell survival and metabolic adaptation via AKT signaling. These network regulates placental endothelial activity, optimizes nutrient allocation, and positively impacts BW.</p><p><strong>Conclusion: </strong>This study clarifies, from a genetic perspective, the causal relationship between PEA and BW. The proposed PEA index is presented as a reliable tool for evaluating placental spatial utilization efficiency in polytocous animal breeding. It offers new genetic insights and a potential approach for improving litter viability.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"250992"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147607616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detailed analysis of inbreeding in Tibetan sheep populations based on genome re-sequencing.","authors":"Lixia Sun, Chao Yuan, Tingting Guo, Bowen Chen, Zengkui Lu, Jianbin Liu","doi":"10.5713/ab.250600","DOIUrl":"10.5713/ab.250600","url":null,"abstract":"<p><strong>Objective: </strong>This study investigates runs of homozygosity (ROH) in 11 Tibetan sheep using resequencing technology. The goal is to assess the inbreeding status and the timing of inbreeding events in Tibetan sheep. The study aims to deepen our understanding of the genomic diversity of Tibetan sheep by integrating analyses of gene flow and genetic diversity. The goal is to provide a theoretical foundation for conserving of genetic diversity and the optimizing genetic breeding strategies for Tibetan sheep.</p><p><strong>Methods: </strong>Sequencing data were obtained from 220 Tibetan sheep representing 11 different breeds across the provinces of Gansu, Qinghai, and the Tibet Autonomous Region. The size and distribution of genomic ROH fragments in these 11 Tibetan sheep populations were analyzed.</p><p><strong>Results: </strong>In 11 Tibetan sheep populations, 85% of the ROH were ranged from 0.5 Mb to 1 Mb in length. Only five populations (Kecai sheep, Ganjia sheep [GJ], Qiaoke sheep [QK], Tianjun white sheep [WT], and Tibetan Gangba black sheep) exhibited ROH segments longer than 3 Mb, although these instances were fairly rare. Chromosome length affects the number and size of ROH in the genome. Tibetan sheep have longer chromosomes 1, 2, and 3, leading to a higher occurrence of ROH. The FROH values were high for Tao sheep, GJ, and QK populations, while the lowest for Zhashijia sheep. Tibetan sheep populations harbor individuals with high inbreeding (FROH>0.2), but overall inbreeding levels are low, primarily from earlier generations. The expected heterozygosity in the Tibetan sheep genome exceeds the observed heterozygosity, indirectly confirming the occurrence of inbreeding in Tibetan sheep populations. Gene flow is evident in various Tibetan sheep populations, particularly between Huoerba sheep and WT.</p><p><strong>Conclusion: </strong>The current breeding strategy for Tibetan sheep has room for improvement; therefore, breeding management and genetic diversity conservation should be prioritized in future programs.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"250600"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147607612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol inhibits NLRP3 inflammasome activation to alleviate bovine mastitis by promoting PINK1-mediated mitophagy.","authors":"Junpeng Huang, Longwei Sun, Shujing Tan, Ran Yu, Weiguo Zhao, Chengmin Li","doi":"10.5713/ab.250935","DOIUrl":"10.5713/ab.250935","url":null,"abstract":"<p><strong>Objective: </strong>Negative energy balance in transition cows elevates the circulating concentrations of non-esterified fatty acids (NEFA), potentially leading to mastitis and posing a significant threat to the dairy industry. Resveratrol is a polyphenolic compound with anti-inflammatory properties, yet its role in NEFA-induced inflammation in bovine mammary epithelial cells (BMECs) remains unclear. This study aimed how resveratrol protects against mastitis and its underlying mechanisms.</p><p><strong>Methods: </strong>BMECs were pre-treated with 100 μM resveratrol for 24 h, and then treated with 0.9 mM NEFAs for 4 h, and a PINK1 inhibitor was used to assess the mechanisms involved. Furthermore, a mouse model of mastitis was utilized to evaluate the hepatoprotective effects of resveratrol against mastitis in vivo.</p><p><strong>Results: </strong>Resveratrol significantly attenuated the NEFA-induced inflammatory response, evidenced by reduced levels of NLRP3 inflammasome components (NLRP3, caspase1, IL-1β) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α). Mechanistically, resveratrol promoted mitophagy by upregulating levels of LC3-II, PINK1, and Parkin, while downregulating P62 expression. The anti-inflammatory effect of resveratrol was reversed when PINK1 was inhibited. In vivo experiments confirmed that resveratrol alleviated mammary gland inflammation and enhanced PINK1-mediated mitophagy.</p><p><strong>Conclusion: </strong>This study demonstrates that resveratrol mitigates NLRP3-mediated inflammation by activating PINK1-mediated mitophagy, suggesting its potential as a therapeutic option for mastitis in perinatal dairy cows with negative energy balance.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"250935"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353114/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}