Animal Bioscience最新文献

{"title":"Effects of varying levels of coated cysteamine hydrochloride in diet on growth performance and carcass quality of steers.","authors":"Watcharawit Meenongyai, Kannika Wonapanit, Pichad Khejornsart, Piyamas Phongkaew, Unchan Traithilen, Naroon Waramit, Md Maksudul Haque Helali, Alimul Islam Shimul, Abdullah Al Mamun","doi":"10.5713/ab.24.0863","DOIUrl":"https://doi.org/10.5713/ab.24.0863","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to evaluate the effects of different levels of coated cysteamine HCl (CSH) supplementation on growth performance, carcass traits, and meat quality in Charolais crossbred cattle.</p><p><strong>Methods: </strong>Twenty-four Charolais crossbred steers, aged 24-30 months with an initial body weight of 418 ± 31 kg, were assigned to three dietary treatments: 0% (control), 0.5%, or 1.0% CSH in concentrate. The steers were fed the experimental diets for 200 days.</p><p><strong>Results: </strong>Increasing CSH levels in concentrate diets led to significant increases (p < 0.05) in body weight, hot carcass weight, cold carcass weight, and dressing percentage. CSH supplementation reduced shear force values and cooking loss (p < 0.05). Meat lightness was significantly greater in the 1.0% CSH-supplemented group (p < 0.05). With increasing CSH levels, the proportion of high-value cuts, such as rib set and T-bone, increased (p < 0.05), while the proportion of lower-value cuts, including macreuse, shank, and tendons, decreased (p < 0.05). Non-carcass weights, including the feet, head, digestive tract, and scraps, were significantly higher in the 1.0% CSH-supplemented diet (p < 0.05). The meat's chemical composition did not differ significantly among the treatment groups (p > 0.05). Muscle fiber diameter was significantly larger in the 1.0% CSH-supplemented group (p < 0.05). Monounsaturated fatty acids increased (p < 0.05) with higher CSH levels, whereas polyunsaturated and saturated fatty acids exhibited a significant decrease (p < 0.05).</p><p><strong>Conclusion: </strong>Dietary supplementation with CSH improves growth rate, carcass traits, and meat quality in steers. An inclusion level of 1.0% CSH in concentrate is the optimum feeding dosage, indicating its potential as an effective feed additive for enhancing beef production efficiency.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analysis of muscle transcriptome data identifies key genes influencing intramuscular fat content in pigs.","authors":"Yan Wang, Jiahao Wang, Yanhui Zhao, Xihui Sheng, Xiaolong Qi, Lei Zhou, Jianfeng Liu, Chuduan Wang, Jianliang Wu, Yongchun Cao, Kai Xing","doi":"10.5713/ab.24.0905","DOIUrl":"https://doi.org/10.5713/ab.24.0905","url":null,"abstract":"<p><strong>Objective: </strong>Intramuscular fat (IMF) content is closely linked to meat quality, and the process of IMF deposition is intricate. Despite numerous transcriptomic studies on IMF, varying sample sizes and data analysis methods have led to inconsistent gene expression patterns and results. To identify the pivotal genes influencing pig IMF content, we performed a meta-analysis on 10 pig muscle transcriptome datasets with a total of eighty samples, including forty each of high and IMF samples.</p><p><strong>Methods: </strong>We used DESeq2 to analyze the high and low IMF groups for each of the 10 datasets, identifying differentially methylated genes in each dataset. To identify key genes affecting IMF content, we performed a meta-analysis of the differential expression results from the 10 datasets using MetaVolcanoR. Subsequently, we conducted PPI network analysis, as well as GO and KEGG functional enrichment analysis, and quantified the trait locus (QTL) analysis on the differentially expressed genes (DEGs).</p><p><strong>Results: </strong>The meta-analysis revealed 494 DEGs, with 321 upregulated and 173 downregulated DEGs observed in the high IMF group. These DEGs were found to be enriched in processes associated with adipocyte differentiation and fat anabolism. QTL analysis demonstrated that five DEGs, including FASN and SCD, corresponded to six QTLs associated with IMF.</p><p><strong>Conclusion: </strong>Our findings suggest that meta-analysis can effectively integrate data from multiple datasets to yield more reliable results. Using this approach, we identified the core gene cluster, including FASN, SCD, and PLIN1, LEP, and G0S2, that influence IMF content in pigs.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomics unveils chemical modifications on protein side chains in raw breast meat of broilers (Gallus gallus) affected with growth-related myopathies.","authors":"Yuwares Malila, Sawanya Charoenlappanit, Narumon Phaonakrop, Yanee Srimarut, Sittiruk Roytrakul","doi":"10.5713/ab.24.0892","DOIUrl":"https://doi.org/10.5713/ab.24.0892","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate how growth-related myopathies influenced chemical modifications formed on amino acid residues of chicken breast proteins.</p><p><strong>Methods: </strong>Breasts (pectoralis major) of commercial broilers (Gallus gallus) were classified into \"normal\", White Striping (WS)\" and \"White Striping + Wooden Breast (WS+WB)\" groups (n = 9 per group). The meat was subjected to proteomic analysis using a liquid chromatography-tandem mass spectrometry. Differences in abundance of modified sites, including methylated lysine (Lys) and arginine (Arg), acetsylated Lys, and oxidized methionine (Met), due to the growth-related myopathies were identified (false discovery rate, FDR < 0.05). Biological functions of the proteins were analyzed.</p><p><strong>Results: </strong>Proteomics revealed 185, 105, and 194 modified sites for methylation, Lys acetylation and Met oxidation, respectively. Of 185, 10 sites from seven proteins (TPM1, MYH, MYH1F, DICER1, RCJMB04_5k17, TPI1, and VIM) showed differential abundance in the methylation (FDR < 0.05). Seven acetylated Lys sites from five proteins (TPM1, ADHFE1, SPAG9, PCNT, and RCJMB04_5k17) were differentially expressed. The abundance of those sites in normal samples were lower than those of WS samples (FDR < 0.05). As for oxidized Met, differential 62 sites were identified (FDR < 0.05). The major Met-oxidized protein was MYH. Met oxidation of 40 sites from 22 proteins was increased in WS samples whereas 19 sites of four proteins (MYL11, MYH, MYH1F, and TNNT2) were increased in WS+WB samples. Only four sites from DICER1, LDHA and LDB3 were found in normal samples (FDR < 0.05).</p><p><strong>Conclusion: </strong>The findings shed light on the links between oxidative stress and oxidized Met in the chicken with growth-related myopathies. In addition, methylation and acetylation modifications likely played a role in dynamic cell signaling to maintain cellular activities, particularly metabolism and energy production, against the stress in the affected birds.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery and functional study of lncRNAs associated with fat deposition in Kele pigs based on whole-transcriptome RNA sequencing.","authors":"Xiong Zhang, Yong Guo, Zhonglong Zhao, Tian Song Wang, Qing Shi Wang, Runqian Yang, Zhaobi Ai, Yong Zhang","doi":"10.5713/ab.24.0900","DOIUrl":"https://doi.org/10.5713/ab.24.0900","url":null,"abstract":"<p><strong>Objective: </strong>To screen out the lncRNAs related to fat deposition in Kele pigs and explore their molecular regulatory mechanisms through biological function verification, it is expected to provide a theoretical basis for breeding, development and utilization of Kele pigs.</p><p><strong>Methods: </strong>In this work, 6 healthy 10-month-old Kele pigs were selected as the experimental subjects and divided into high and low backfat thickness groups. Subcutaneous adipose tissue of each group was collected for whole-transcriptome RNA sequencing (RNA-seq) and lncRNAs associated with backfat thickness were randomly selected for real-time quantitative PCR (RT-qPCR) analysis. The correlation analysis between lncRNAs expression and production data was conducted, and the biological function of lncRNAs were identified.</p><p><strong>Results: </strong>A total of 493 lncRNAs with significantly different expression were found by RNA-seq (p<0.05), and it was found that TCONS_00185937 and TCONS_00161198 may be involved in the regulation of fat deposition. The results of RT-qPCR were consistent with RNA-seq, indicating that the sequencing results were reliable. Correlation analysis showed that the expression of TCONS_00185937 was significantly negatively correlated with backfat thickness, and the expression of TCONS_00161198 was significantly positively correlated with backfat thickness, and negatively correlated with eye muscle area (p<0.05). The pEGFP-TCONS_00185937 was transfected into subcutaneous fat precursor cells, it was found that the expression levels of TCONS_00185937 and its target genes MOGAT2 and ATGL were significantly higher than control group (p<0.05). Similarly, pEGFP-TCONS_00161198 transfected cells found that the expression levels of TCONS_00161198 and its target genes CSF3R and ACC were significantly higher than control group (p<0.05). The induced differentiation of transfected cells found that the OD510 absorption value of pEGFP-TCONS_00161198 group was higher than that of the control group. Triglyceride content was significantly higher than that of control group (p<0.01), while OD510 absorption value and triglyceride content of pEGFP-TCONS_00185937 group were lower than that of control group.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"L-Lysine Improves Pork Quality During Postmortem Aging: Insights into Meat Quality, Protein Properties, and Enzyme Activities.","authors":"Xiuyun Guo, Shuangyi Xu, Chao Fu, Xiangren Meng","doi":"10.5713/ab.24.0901","DOIUrl":"https://doi.org/10.5713/ab.24.0901","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the intrinsic relationships between meat quality, protein properties, and enzyme activities of pork longissimus dorsi treated with L-lysine (Lys) during postmortem aging.</p><p><strong>Methods: </strong>The pork was obtained from 18 twelve-month-old crossbred pigs (120 kg, Duroc × Long White Large × White, longissimus dorsi muscle) in three batches of six samples each. The meat was then immediately placed in a thermal container and transported to the laboratory within 1 hour for subsequent processing at 4 ℃. After removing fat and connective tissue, the pork was cut into 20 g ± 1 g meat blocks. Then, the samples were vacuum sealed and left in a freezer (4 ℃) for 0, 1 and 3 days.</p><p><strong>Results: </strong>The results indicated that Lys addition (0.10%, 0.15%, and 0.20%) could improve pork quality (water-holding capacity and tenderness). On the third day of aging, the shear force values reached their lowest levels (P < 0.05), measuring 38.21 N, 34.04 N, and 30.94 N for the respective treatment groups. In addition, the postmortem addition of Lys significantly increased the myofibrillar fragmentation index and actomyosin solubility during pork aging (P < 0.05), with maximum values of 105.07 and 90.35%, respectively. Meanwhile, microscopic structure and electrophoresis results indicated that Lys treatment disrupted the muscle fiber structure, promoted the degradation of MPs (myofibrillar proteins) and dissociation of actomyosin. Furthermore, with increasing Lys addition, calpain-1 activity, caspase-3 activity, and Ca2+-ATPase activity in the muscle significantly increased (P < 0.05), reaching maximum values of 17.46 ng/mL, 55.68 µg/mL, and 2.79 μmol (Pi)/min·mg protein, respectively. The activation of these enzymes promoted the dissociation and hydrolysis of key structural proteins.</p><p><strong>Conclusion: </strong>Lys improved pork quality by activating calpain-1, caspase-3, and Ca2+-ATPase activity during the postmortem aging, thereby promoting the degradation of MPs and the dissociation of the actomyosin.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary crude protein levels on growth performance, rumen characteristics, blood metabolites, and methane emissions in finishing Hanwoo steers.","authors":"Joonpyo Oh, Hyunjin Cho, Namkyu Kang, Mingyung Lee, Md Raihanul Hoque, Yoo Yong Kim, Seongwon Seo","doi":"10.5713/ab.25.0063","DOIUrl":"https://doi.org/10.5713/ab.25.0063","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to investigate the effects of increasing dietary crude protein (CP) concentration on growth performance, rumen characteristics, blood metabolites, and methane (CH4) emissions in finishing Hanwoo steers.</p><p><strong>Methods: </strong>Twenty-four 26-month-old Hanwoo steers (717±50.1 kg) were utilized in a 12-week study based on a completely randomized block design. The animals were randomly assigned to one of four dietary treatments by feeding concentrate mixes with different CP levels (15.0, 16.2, 17.5, and 18.5% on a dry matter [DM] basis). Forage was fed ad libitum, while the concentrate mix was provided in a fixed amount.</p><p><strong>Results: </strong>Forage and total dry matter intake (DMI) linearly increased (P ≤ 0.029) with increasing CP levels while concentrate intake was not affected by treatments. Initial and final body weight and average daily gain were not different among treatments. Feed efficiency showed a trend of linear decrease (P = 0.092) with increasing CP levels. Rumen parameters, including ruminal pH, total volatile fatty acid, molar proportions of acetate, propionate, isobutyrate, butyrate, isobutyrate, valerate, and acetate to propionate ratio, were not affected by treatments. Treatments also did not affect blood concentrations of total protein, urea, glucose, non-esterified fatty acid, albumin, creatinine, triglyceride, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, calcium, and phosphorus. Concentrations of CH4 from respiration or eructation were not affected by dietary CP. Methane ppm per DMI and neutral detergent fiber (NDF) intake was not also different among treatment in respiration or eructation. However, CH4 ppm per forage NDF intake linearly decreased (P ≤ 0.005) with increasing CP from both respiration and eructation.</p><p><strong>Conclusion: </strong>These findings provide no clear evidence that increasing the CP content of the concentrate mix from 15.0 to 18.5% DM improves growth performance and body metabolism in finishing Hanwoo steers. Nevertheless, they suggest that a higher CP level may negatively affect feed efficiency.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploitation of Pig By-product: Physicochemical Properties and Flavor of Dachang Tang - Effect of Cooking Time.","authors":"Jiayin Wei, Tongxiang Yang, Xiangtian Wu, Yunfei Wang, Kongyang Wu, Xu Duan, Wenchao Liu, Weiwei Cao, Linlin Li, Chung Lim Law, Junliang Chen, Guang Yue Ren","doi":"10.5713/ab.24.0894","DOIUrl":"https://doi.org/10.5713/ab.24.0894","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to explore the optimal cooking time for Dachang tang, both to promote Chinese cuisine culture and to improve the utilization of pig large intestines, thereby reducing resource waste.</p><p><strong>Methods: </strong>The raw pig large intestines were cleaned with water and flour, then boiled with selected spices for 40 mins to reduce off-odors. Subsequently, the large intestines were stir-fried with oil, garlic, and zanthoxylum bungeanum for a specified duration, before being added to the original broth. The pig large intestines were then cooked for 10 mins, 20 mins, 40 mins, and 60 mins, respectively. Samples were collected at each cooking times to analyze their basic nutritional components (moisture, protein, fat), texture, color, water state, fatty acids, volatile compounds, microstructure, and sensory characteristics.</p><p><strong>Results: </strong>With the increase in cooking time, the moisture and fat content decreased significantly, and the ratio of unsaturated to saturated fatty acids (UFA/SUFA) increased (p<0.05). At 20 mins, pig large intestines exhibited optimal chewiness, color, surface integrity and sensory scores. Meanwhile, volatile flavor compounds mainly derived from spices, such as 4-methoxybenzaldehyde, benzaldehyde, 3-ethyltoluene, estragole, and ethyl decanoate effectively masking the off-flavor caused by p-cresol and indole in pig large intestines, and provided fruity, spicy, fennel and citrus aromas to Dachang tang.</p><p><strong>Conclusion: </strong>A comprehensive analysis concluded that 20 mins was the optimal cooking time.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parental betaine supplementation promotes hepatic conversion of cholesterol to bile acids in offspring goslings with epigenetic modulation of CYP7A1 gene.","authors":"Shuai Ma, Liang Chen, Yan Wang, Lei Wu, Ruqian Zhao","doi":"10.5713/ab.24.0857","DOIUrl":"https://doi.org/10.5713/ab.24.0857","url":null,"abstract":"<p><strong>Objective: </strong>Maternal betaine supplementation has been shown to affect offspring metabolism through epigenetic mechanisms in mammals. This study aimed to investigate whether parental betaine supplementation can exert intergenerational effects on hepatic cholesterol metabolism in offspring goslings, and the possible epigenetic modification associated with such effects.</p><p><strong>Methods: </strong>In this study, 450 female and 90 male Jiangnan White goose breeders, aged 39 wks, were randomly assigned to three groups receiving basal (Control, CON), or betaine-supplemented diets at low (2.5 g/kg, LBT) or high (5 g/kg, HBT) levels for 7 weeks. The breeder eggs laid in the last week were collected for incubation. Offspring goslings were slaughtered at 35 and 63 days of age to collect blood, bile, and liver samples for biochemical analysis and gene and protein expression studies.</p><p><strong>Results: </strong>The body weight of goslings in the LBT and HBT groups was significantly higher than that in the CON group (p < 0.05). Serum and liver total cholesterol (TCHO) contents were significantly decreased in the HBT group (p < 0.05), while the total bile acids (TBA) contents in the liver and bile were significantly increased (p < 0.05). These changes were associated with the upregulation of cholesterol-7 alpha-hydroxylase (CYP7A1) and bile salt export protein (BSEP) at both mRNA and protein levels (p < 0.05). Additionally, parental betaine supplementation significantly decreased the DNA methylation level on the promoter region of the CYP7A1 gene (p < 0.05).</p><p><strong>Conclusion: </strong>These results indicate that parental betaine supplementation decreases hepatic cholesterol content in offspring goslings through epigenetic modulation of the CYP7A1 gene.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyrroloquinoline quinone promotes porcine oocyte in vitro maturation and subsequent embryo development by enhancing lipid metabolism and improving mitochondrial func-tion.","authors":"Zehua Zhang, Zhigang Gao, Zhenwei Jia","doi":"10.5713/ab.24.0847","DOIUrl":"https://doi.org/10.5713/ab.24.0847","url":null,"abstract":"<p><strong>Objective: </strong>The present study evaluated the beneficial effects of pyrroloquinoline quinone (PQQ) on porcine oocyte in vitro maturation (IVM) and early embryo development.</p><p><strong>Methods: </strong>Porcine cumulus oocyte complexes were cultured in IVM medium supplemented with 0, 200 nM, 400 nM, 800 nM or 1600 nM PQQ for 42 h. We first examined cumulus expansion index (CEI) and oocyte nuclear maturation rate, and then assessed oocyte mitochondrial function, oxidative stress levels, lipid metabolism and subsequent embryonic development.</p><p><strong>Results: </strong>We found that PQQ (800 nM) supplementation significantly increased CEI and the nuclear maturation rate of oocytes after IVM. Furthermore, PQQ (800 nM)-supplemented oocytes showed significantly higher mitochondrial content, mitochondrial membrane potential (MMP) and activity, and the mRNA expression levels of genes related to mitochondria biogenesis (PGC-1a, NRF1, NRF2 and TFAM). Moreover, PQQ significantly reduced the levels of reactive oxygen species (ROS), lipid droplets and fatty acids content, and increased the mRNA expression levels of genes related to antioxidant (SOD1, SOD2, GPX and CAT), lipolysis (ATGL and HSL) and β-oxidation (CPT1B and CPT2) in porcine oocytes. Ultimately, PQQ (800 nM) supplementation significantly increased cleavage rate, blastocyst formation rate, and total blastocyst cell numbers after parthenogenetic activation.</p><p><strong>Conclusion: </strong>PQQ supplementation during IVM exerts a positive influence on oocyte maturation quality and subsequent embryo development by enhancing mitochondrial function and lipid metabolism and alleviating oxidative stress.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incorporation of unfermented or fermented de-oiled rice bran meal into a rabbit's diet impacts growth performance, nutrient digestibility, cecal microbiota.","authors":"Shehata Zeid, Sindaye Daniel, Liao Jinghong, Suqin Hang","doi":"10.5713/ab.24.0890","DOIUrl":"https://doi.org/10.5713/ab.24.0890","url":null,"abstract":"<p><strong>Objective: </strong>This study assessed the effects of incorporating unfermented or fermented rice bran meal (RBM) into a rabbit's diet on their growth performance, cecal microbiota, and intestinal health.</p><p><strong>Methods: </strong>A total of 21 male weaned New Zealand White rabbits aged 6 weeks were randomly allocated into 3 groups (n = 7). Rabbits consumed the basal diet in the CON group and the basal diet with a 20% substitution of either unfermented RBM (UFRBM group) or fermented RBM (FRBM group) for 8 weeks. The RBM was fermented by a combination of Lactobacillus johnsonii L63 with hydrolytic enzymes (cellulase (100 U/g), phytase (1.25 U/g), and papain (300 U/g)) and incubated for 60 hours at 37 °C and a pH value of 4.8.</p><p><strong>Results: </strong>Our results demonstrated that the rabbits' growth performance (FBW, ADG, and FCR), serum biochemical variables, and cecal microbiota for α-diversity and β-diversity at the phylum level didn't differ among the treatments (P ˃ 0.05). The FRBM increased nutrient digestibility, antioxidant capacity, intestinal histomorphology, digestive enzymes, nutrient transporter expression, short-chain fatty acid (SCFA) concentration, and the abundance of glycolysis/gluconeogenesis and carbohydrate digestion and absorption metabolic pathways than those in the CON group (P ˂ 0.05), while it was improved in most parameters than the UFRBM. The UFRBM enhanced the ADFI, ether extract digestibility, jejunal villi height, solute carriers of family 3 member 1 (SLC3A1), family 1 member 1 (SLC1A1), occludin (OCLN) up-regulations, SCFA, the relative abundance of V9D2013 group and Rikenella at the genus level, and the glycolysis/gluconeogenesis metabolic pathways, and lowered the malonaldehyde (MDA) concentration than those in the CON group (P ˂ 0.05).</p><p><strong>Conclusion: </strong>The two formulas incorporating 20% of UFRBM or FRBM in rabbit diet should be recommended to farmers, particularly the FRBM, to decrease the reliance on corn and soybean meal for rabbit production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}