Animal Bioscience最新文献

{"title":"Effects of Saccharomyces cerevisiae fermentation products on growth performance, fecal short chain fatty acids, and microbiota of pre-weaning calves.","authors":"Qian Lei, Zhiqiang Cheng, Maocheng Jiang, Qianbo Ma, Xiaoxiao Gong, Yongjiu Huo, Miao Lin","doi":"10.5713/ab.24.0340","DOIUrl":"10.5713/ab.24.0340","url":null,"abstract":"<p><strong>Objective: </strong>This research aims to explore the effects of incorporating Saccharomyces cerevisiae fermentation products (SCFP) on growth performance, nutrient digestibility, antioxidant capacity, fecal short-chain fatty acids, and microbial composition of pre-weaning calves.</p><p><strong>Methods: </strong>Twenty Holstein calves, 10 days old and weighing an average of 48.63±0.91 kg, were randomly assigned to either the control group (CON) or the SCFP group, with 10 calves in each group. The CON group received only a basal diet, while the SCFP group received the starter diet supplemented with 5 g/head/d of SCFP products. The pre-trial period lasted for 5 days, followed by a main experimental period of 45 days.</p><p><strong>Results: </strong>The SCFP group had significantly higher final weight, average daily gain, and feed efficiency compared to the CON group (p<0.05). Moreover, the SCFP group exhibited increased apparent digestibility of dry matter, crude protein, ether extract, acid detergent fiber, Ca, and P (p<0.05). Additionally, supplementation with SCFP led to elevated content of growth hormone, insulin-like growth factor-1, and glucagon-like peptide-1 in serum. The inclusion of SCFP also raised serum catalase content and reduced serum malondialdehyde content in pre-weaning calves. Furthermore, SCFP supplementation influenced the composition of intestinal microflora by decreasing Actinobacteriota abundance and increasing the abundance of Ruminococcus, Lachnospiraceae_AC2044_group, Parabacteroides, and Butyricimonas.</p><p><strong>Conclusion: </strong>The addition of SCFP has a positive impact on the growth performance, nutrient digestibility, antioxidant capacity, and intestinal microflora composition of pre-weaning calves.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"955-967"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of stocking densities on growth, organ index, serum biochemistry, gut morphology and microbiota of young ducks in a rice-duck-crayfish coculture system.","authors":"Xiao Long He, Zhen Hua Liang, Ze Heng Huang, Lian Bing Qi, Yan Wu, Jia Liu, Tao Huang, Jing Bo Liu, Jin Song Pi, Hao Zhang","doi":"10.5713/ab.24.0488","DOIUrl":"10.5713/ab.24.0488","url":null,"abstract":"<p><strong>Objective: </strong>The rice-duck-crayfish (RDC) coculture system, an ecologically efficient breeding strategy that accommodates natural behavior of ducks and improves their welfare. The optimal stocking density and its impact on duck health in this system remains undetermined. The study examined the impact of stocking densities on growth, organ index, serum biochemistry, gut morphology and microbiota of ducks in RDC system.</p><p><strong>Methods: </strong>A total of five hundred and forty 20-day-old Nonghu No. 2 ducks were randomly allocated based on density: low-density (LD; 8 birds/666.67 m2), mediumdensity (MD; 12 birds/666.67 m2) and high-density (HD; 16 birds/666.67 m2) groups, with three replicates in each group, and the symbiosis period was up to 40 days until rice tasselling.</p><p><strong>Results: </strong>There were no significant differences in final body weight, average daily gain, or feed:gain ratio between groups (p>0.05); however, the liver and spleen indices of ducks in HD group were significantly greater than those in LD group (p<0.05). The serum albumin concentration in HD group decreased, whereas creatine kinase activity increased (p<0.05). Additionally, the ileal crypt depth significantly increased and the ileal villus height and villus/crypt ratio significantly decreased in ducks in MD and HD groups compared to LD group (p<0.05). Moreover, the abundance of cecal Deferribacterota and Spirochaetota increased significantly (p<0.05), while the abundance of Firmicutes decreased significantly (p<0.05) with increasing stocking density. Moreover, the increase in stocking density significantly decreased the abundance of some beneficial bacteria (Faecalibacterium and Fournierella) and increased the abundance of some harmful bacteria (Mucispirillum and Brachyspira) (p<0.05).</p><p><strong>Conclusion: </strong>These results suggest that moderately HD breeding doesn't significantly affect duck growth, but increased stocking density led to changes in cecal microbiota and dysbiosis. Reducing stocking density positively affects immune parameters and ileum morphology. However, due to the limited number of total replicates of the study, further research is needed to validate the reliability of the results.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1067-1080"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alteration of growth performance, glycolipid metabolism, and bone characteristics of broiler chickens in response to different inclusion levels of dietary wheat.","authors":"Yilu Wang, Leilei Wang, Xuemeng Si, Yanqun Huang, Huaiyong Zhang, Wen Chen","doi":"10.5713/ab.24.0256","DOIUrl":"10.5713/ab.24.0256","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to evaluate the effects of different levels of wheat inclusion on growth performance, glycolipid metabolism, and tibial properties of broiler chickens.</p><p><strong>Methods: </strong>A total of 480 1-d-old male broiler chickens were initially fed identical starter diets until d 10. Subsequently, they were divided into 3 treatments consisting of 8 replicates with 20 birds per replicate, i.e., i) low-level wheat addition group, with wheat ratios of 15% and 25% during the grower and finisher periods, respectively; ii) medium-level wheat inclusion group, incorporating 30% and 40% wheat in the grower and finisher diets, respectively; and iii) high-level wheat addition group, containing 55.8% and 62.4% wheat in the grower and finisher diets, until d 39.</p><p><strong>Results: </strong>When compared to the low- and medium-level wheat diet, the high-level wheat inclusion in the diet increased feed intake and reduced the feed conversion ratio (both p<0.01), which was accompanied by a longer jejunum (p = 0.031). Meanwhile, the high-level addition of wheat displayed a decreased abundance of Ruminococcin, Bacteroidetes, and Lactobacillus than the low-wheat group. With the increase of the proportion of wheat treatment, the contents of cholesterol, triglyceride, and high-density lipoprotein cholesterol were elevated in serum, whereas the concentration of serum C-terminal cross-linked telopeptide of type I collagen, a bone resorption marker, was decreased. In addition, the diet with medium and high levels of wheat improved the yield load of tibia, along with comparable bone dimension and weight.</p><p><strong>Conclusion: </strong>The medium- and high-level wheat additions increased serum glycolipid deposition and enhanced tibial mechanical properties, whereas the high-level wheat diet compromised the growth performance of broiler chickens, which might be associated with the alteration of gut microbiota.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1015-1028"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary sodium sulfate supplementation improves eggshell quality, uterine ion transportation and glycosaminoglycan synthesis in laying hens.","authors":"Kai-Bo Fu, Dong Dai, Jian-Min Zhou, Jing Wang, Hai-Jun Zhang, Shu-Geng Wu, Guang-Hai Qi, Jing Wang","doi":"10.5713/ab.24.0456","DOIUrl":"10.5713/ab.24.0456","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the effects of dietary sodium sulfate (Na2SO4) supplementation on eggshell quality, uterine ion transportation, and glycosaminoglycan (GAG) synthesis.</p><p><strong>Methods: </strong>A total of 432 48-wk-old Hy-line Brown laying hens were randomly divided into 6 dietary treatments with 8 replicates of 9 birds each. The experimental laying hens were fed the corn-soybean meal diets (containing 0.15% NaCl) supplemented with 0.22%, 0.37%, 0.52%, 0.68%, 0.83%, or 0.99% Na2SO4 for 12 weeks.</p><p><strong>Results: </strong>Results showed that the eggshell breaking strength and eggshell ratio significantly increased in the 0.68% Na2SO4 group at the end of wk 56 and wk 60 (p<0.05). In addition, eggshell thickness and weight significantly increased in the 0.68% Na2SO4 group at the end of wk 60 (p<0.05). Eggshell calcium content in the 0.68% Na2SO4 group was higher than that of 0.22% and 0.99% groups (p<0.001). The concentrations of K+ and Ca2+ in the uterine fluid were significantly greater in the 0.68% group than in the other groups (p<0.05). Dietary Na2SO4 increased the gene expression of SLC8A1, SCNN1A, ATP1B1, and KCNMA1 quadratically in the uterus (p<0.05), and higher values were observed in 0.68% group. Additionally, the GAG contents of the eggshell, and ATP-sulfurylase, sulfotransferase, chondroitin sulfate, and dermatan sulfate contents of the isthmus increased linearly with the increment of dietary Na2SO4 (p<0.05). There was a remarkable reduction in mammillary knob width, mammillary thickness, and the percentage of the mammillary layer (p<0.05), and an increment in mammillary knob density, effective thickness, and total thickness in the 0.68% group compared with the 0.22% and 0.99% groups (p<0.05).</p><p><strong>Conclusion: </strong>Overall, there was no dose-related difference with the increment of dietary Na2SO4 levels. The addition of 0.68% Na2SO4 in the corn-soybean basal diet (0.15% Cl) regulated uterine ion transport, increased GAG contents of eggshell, and improved eggshell ultrastructure and quality.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1029-1040"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin regulates rabbit sperm motility and kinematics via the MT1/PKC signaling pathway.","authors":"Chongchong Wang, Yanyan Zhang, Shiwen He, Biao Jiang, Jinglei Huang, Hui Peng","doi":"10.5713/ab.24.0593","DOIUrl":"10.5713/ab.24.0593","url":null,"abstract":"<p><strong>Objective: </strong>Melatonin, a highly conserved molecule, plays an essential role in various physiological functions. Research suggests that incorporating melatonin into semen extender enhances livestock sperm viability. However, the effect of melatonin on rabbit sperm and the molecular mechanisms underlying melatonin-regulated rabbit sperm motility and kinematics remain unclear. This study aimed to reveal the molecular mechanism by which melatonin regulates rabbit sperm motility and kinematics.</p><p><strong>Methods: </strong>This study investigated the expression and localization of melatonin-related proteins in rabbit testis and epididymis. Rabbit sperm was incubated at different concentrations of melatonin for 60 min or 90 min at 37°C, followed by an evaluation of sperm motility parameters using IVOS II computer assisted sperm analyzer system (CASA). Then we examined the integrity of the sperm plasma membrane, mitochondrial membrane potentials and intracellular reactive oxygen species. Furthermore, melatonin receptor antagonists were added to the extender and investigated the involvement of the melatonin receptors in the regulation of sperm motility parameters. We carried out phosphoproteomics analysis and verified regulation of rabbit sperm kinematics by melatonin via the inhibition of potential signaling pathway.</p><p><strong>Results: </strong>Melatonin-related proteins were expressed and localized in testes and epididymis of rabbits. Melatonin (5 mM) markedly increased rabbit sperm motility, kinematics, and overall sperm quality. Melatonin regulated rabbit sperm motility and kinematics via the MT1 receptor. Phosphoproteomics combined with GPS 5.0 software revealed the potential signaling pathway by which melatonin regulates sperm kinematics. Moreover, the inhibition of protein kinase C (PKC) markedly reduced rabbit sperm kinematics, whereas the inhibition of ERK1/2, p38 MAPK, PKG and JNKs using kinase inhibitors did not result in obvious changes in sperm kinematics. In addition, inhibition of the MT1 receptor significantly weakened PKC activity, suggesting that PKC is downstream of MT1.</p><p><strong>Conclusion: </strong>We conclude that melatonin regulates rabbit sperm kinematics through the MT1/PKC signaling pathway.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"929-940"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Type I and III collagen contents and μ-calpain autolysis as a function of dry ageing time for eight different muscles from Hanwoo cattle.","authors":"Zhen Song, Inho Hwang","doi":"10.5713/ab.24.0376","DOIUrl":"10.5713/ab.24.0376","url":null,"abstract":"<p><strong>Objective: </strong>Type I and III collagen content exert contrasting influences on meat tenderness. μ-calpain autolysis correlates with beef tenderness. Thus, the study aimed to determine the changes in these proteins.</p><p><strong>Methods: </strong>Three hundred twenty-four Hanwoo cattle, including cows and steers, and eight muscles were evaluated for proteolysis during dry ageing period. The ratios of type I and III collagen were determined by densitometric scans of bands resolved by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE), and μ-calpain activity was determined using casein zymography. Proteins involved in proteolysis were analysed by liquid chromatography-tandem mass spectrometry.</p><p><strong>Results: </strong>The ratio of type I and III collagen in every muscle showed a significant difference with increasing ageing times (p<0.05). In steers, the ratio decreased with increased ageing time, and in cows, except for Biceps femoris and Diaphragm muscles, a similar trend was observed. Significant differences in the ratio of type I and III collagen were found between different muscles of cows at the same ageing time (p<0.05), but no significant differences were found in steer muscles at the same ageing time (p>0.05). Casein zymogram results showed an inverse relationship between pH values and μ-calpain autolysis in every muscle. A significant reduction in μ-calpain activity was observed in all muscles with extended ageing times, while the rate of autolysis differed greatly (p<0.05) between muscles at the same ageing time. Interestingly, electropherogram analysis showed that cow muscles had a higher μ-calpain activity than steer muscles. Ageing time significantly influenced proteolysis, with 24 proteins showing marked changes.</p><p><strong>Conclusion: </strong>The ageing times significantly affect the ratio of type I and III collagen, coinciding with μ-calpain autolysis rates in steers. The ratio of type I and III collagen had a significant changes during the ageing period from cows, which may be related to the amount of collagen cross-linking.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1041-1052"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062827/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of probiotic supplementation and rabbit line on growth performance, carcass yield, blood biochemistry and immune response under hot weather.","authors":"Magdy Abdelsalam, Moataz Fathi, Alaa El-Raffa, Ghada Abd El-Latif, Osama Abou-Emera, Mohamed Abd El-Fatah, Gamal Rayan","doi":"10.5713/ab.24.0904","DOIUrl":"https://doi.org/10.5713/ab.24.0904","url":null,"abstract":"<p><strong>Objective: </strong>An experiment was conducted to investigate the beneficial effect of probiotic supplementation via drinking water on growth performance, carcass yield, blood parameters, and immunity of two rabbit lines during the summer season.</p><p><strong>Methods: </strong>In a 6-week probiotic supplementation trial, a total of 90 healthy growing rabbits having a similar weight (590.3±16 g) representing two lines (V-Line and Alexandria) were randomly allocated into three equal groups. Three levels of probiotics (0, 6.67 × 109 CFU/L and 13.35 × 109 CFU/L of Bacillus licheniformis) were administered through drinking water as non-supplemented, low, and high levels.</p><p><strong>Results: </strong>A linearly increased body weight at 5 and 6 weeks of the experiment (p=0.01 and p<0.01, respectively) were noticed with the probiotic supplementation at high level. The same trend was noticed for the overall body weight gain (p=0.05). Probiotic supplementation did not affect carcass traits, except for heart and lungs, where the rabbits that received the high level recorded higher weights (p=0.01 and p<0.01, respectively). Probiotic supplementation showed a linearly effect (p=0.02) on the cell-mediated index after 24 h of PHA-P injection. Lower triglyceride concentration (p=0.04) was observed in the high group compared to the other groups. Concerning the line rabbit, although the Alexandria rabbits (Alex) recorded lighter body weight, they exhibited a better gain-to-feed ratio (p=0.05) compared to the V-line ones. Also, an increase in heart weight and spleen index (p<0.01) was found in V-Line rabbits compared to the Alex ones. Contrary, the Alex rabbits recorded a higher kidney weight (p<0.01) than that of V-Line. Overall, no difference was recorded between the rabbit lines in immune response.</p><p><strong>Conclusion: </strong>The beneficial effects of probiotic supplementation via drinking water on rabbit performance under hot weather were identified as improved growth performance, an enhanced lipid profile, and an early increase in cell-mediated immunity in both rabbit lines that received a high level of the probiotics.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Mongolian 'Bergenia crassifolia L. (root)' extract on rumen methane emission reduction and microbial community.","authors":"Xinrui Zhao, Otgonpurev Sukhbaatar, Linlin Kou, Xinming Cheng, Metha Wanapat, Mahmoud Kamal, Togtokhbayar Norovsambuu, Zhanying Sun, Yanfen Cheng","doi":"10.5713/ab.24.0836","DOIUrl":"https://doi.org/10.5713/ab.24.0836","url":null,"abstract":"<p><strong>Objective: </strong>The study investigates the effects of Mongolian plants on reducing methane emissions, fermentation parameters, and microbial communities in grazing systems.</p><p><strong>Methods: </strong>Initially, various Mongolian plants were screened to identify those that effectively reduced methane production in vitro. The most effective plant was selected for the extraction of its active components, resulting in a solid extract with a quantified concentration of its bioactive compounds. Extracts of 0, 1.0, 2.0, 3.0, 4.0, and 5.0% of DM were added, respectively, and then subjected to in vitro rumen fermentation trials using rice straw and alfalfa as substrates to evaluate their methane reduction effects. Key metrics assessed included gas production, methane production, and rumen fermentation parameters. After the fermentation experiments, we extracted DNA from the rumen fluid for quantitative PCR and 16S high-throughput sequencing analysis.</p><p><strong>Results: </strong>Bergenia crassifolia L. (root) was identified as the plant exhibiting the most significant methane reduction capability with approximately 70% condensed tannins in its extract. This extract significantly decreased methane production in the rice straw substrate (p<0.01), while only the highest dose had a notable effect in the alfalfa substrate (p<0.01). Furthermore, low doses of the extract did not significantly impact rumen fermentation parameters. The genera Ruminococcus and Christensenellaceae R-7 were identified as the principal contributors to the reduction of methane emissions (p<0.01).</p><p><strong>Conclusion: </strong>Bergenia crassifolia L. (root) was more effective in reducing methane emissions from low-quality roughage compared to high-quality roughage, with an optimal addition concentration of 1.0% for low-quality feed. This plant presents a promising new strategy for mitigating rumen methane emissions in grazing systems.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of combined cryoprotectants on the cryotolerance of boar sperm.","authors":"Shuangyi Deng, Liwei Yang, Li Gao, Chengcheng Ning, Shiyin Wang, Wei Zhang","doi":"10.5713/ab.24.0915","DOIUrl":"https://doi.org/10.5713/ab.24.0915","url":null,"abstract":"<p><strong>Objective: </strong>The frozen semen has the significant advantages of long-term storage and long-range transportation. However, due to the low cryotolerance of boar sperm, the global utilization of frozen boar semen in artificial insemination was less than 1% until the year 2000.</p><p><strong>Methods: </strong>In this study, the effects of five cryoprotectants at different concentrations on the cryotolerance of boar semen were evaluated when they were added separately, and the optimal concentrations for each cryoprotectant were determined, then their combined additive effects were further assessed.</p><p><strong>Results: </strong>At a GLY concentration of 5%, the quality of frozen-thawed sperm reached its maximum value, which was significantly higher than the 4% GLY group (p<0.05) and 0% GLY group (p<0.01). The VSL, VCL, VAP, SPMI, SAI and SMA of the frozen-thawed sperm in TEY group exhibited significant improvements compared to the UTEY group (p<0.05). The TM, PM, SPMI, SAI, and SMA of 2% ESP group were significantly higher than the rest groups (p<0.05). The TM, PM, VSL, VCL, and VAP of frozen-thawed sperm in the 250 nM and 300 nM MitoQ groups showed significant improvements compared to the other groups (p<0.05), and the ROS levels in sperm cells were also significantly lower (p<0.05). The quality of frozen-thawed boar sperm in 0.6 mM EGT group reached its peak value and was significantly higher than the rest groups (p<0.05). When these five cryoprotectants were used in combination, the quality of frozen-thawed boar sperm exhibited a significant improvement compared to when they were used individually (p<0.05). Utilizing the frozen-thawed boar semen to inseminate estrus sows, the reproductive performance of the sows did not differ significantly from the sows inseminated with fresh semen (p>0.05).</p><p><strong>Conclusion: </strong>The optimized boar semen cryopreservation system can substantially enhance the quality of frozen-thawed boar sperm, making it suitable for artificial insemination in pig farm.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive meta-analysis on the efficacy of growth factor enriched cryo-agents in enhancing post-thaw quality of mammalian semen.","authors":"Suyatno Suyatno, Herdis Herdis, Tri Puji Priyatno, Mulyoto Pangestu, Santoso Santoso, Tatan Kostaman, Mohammad Firdaus Hudaya, Pangda Sopha Sushadi, Florentina Bety Indah Lupitasari, Anita Hafid, Ita Margaretha Nainggolan, Zultinur Muttaqin, Mohammad Miftakhus Sholikin, Pradita Iustitia Sitaresmi","doi":"10.5713/ab.24.0898","DOIUrl":"https://doi.org/10.5713/ab.24.0898","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the impact of growth factors (GFs) in semen extenders on the quality of post-thaw mammalian sperm using a meta-analysis approach. The primary objective was to determine whether the addition of GFs could enhance semen quality following cryopreservation.</p><p><strong>Methods: </strong>A meta-analysis of various in vitro experiments using mammalian semen was conducted. Data were collected from multiple studies assessing the effects of GFs on sperm motility, viability, acrosome integrity, DNA integrity, and other key semen quality parameters. The analysis included a range of mammalian species, with specific GFs added to semen extenders during cryopreservation. Sperm quality was evaluated through parameters such as motility, viability, acrosome integrity, PMI, DNA integrity, HOST, MDA, and CASA. Statistical analyses, including standardized mean differences (SMD), were performed to compare the effects of GF additives versus control treatments.</p><p><strong>Results: </strong>The addition of GFs to semen extenders significantly improved semen quality across multiple parameters, including motility, viability, acrosome integrity, DNA integrity, and others. Specifically, SMDs for motility, viability, acrosome integrity, PMI, DNA integrity, HOST, MDA, and CASA parameters were significantly higher in the GF-treated groups compared to the controls, with values such as 2.56±0.303, 3.53±0.423, 1.22±0.351, 1.82±0.362, 8.73±2.514, 2.02±0.426, and 6.30±2.87, respectively. Notably, the addition of GFs maintained semen quality in most mammalian species (p < 0.05, SMD >0.5), with the exception of boar semen.</p><p><strong>Conclusion: </strong>The study concluded that the addition of GFs to semen extenders significantly preserves semen quality during cryopreservation in most mammalian species. This improvement is likely attributed to the antioxidants and repair factors present in the GFs. Each GF appeared to exert a distinct effect on sperm, thereby enhancing sperm viability post-thaw. These findings have important implications for improving reproductive technologies in mammalian species, particularly in cryopreservation and artificial insemination protocols.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}