Animal Bioscience最新文献

{"title":"Multiomics reveals changes in lipid metabolism in the livers of landes geese before and after overfeeding.","authors":"Weiqing Ma, Liu Yang, Yadi Jing, Pengwei Ren, Xiang Liu, Meixia Zhang, Xiaomin Qi, Mingxia Zhu, Qiaomei Zhang","doi":"10.5713/ab.25.0405","DOIUrl":"https://doi.org/10.5713/ab.25.0405","url":null,"abstract":"<p><strong>Objective: </strong>: The aim of this experiment was to integrated production indices with omics sequencing to elucidate the systemic perturbations between hepatic metabolism and the gut microbiota during overfeeding.</p><p><strong>Methods: </strong>: A total of 120 seven-week-old male Landes geese were floor reared in a pen environment. Overfeeding commenced at week 8 using a corn-based diet containing 5% soybean oil. The feeding regimen consisted of three daily meals (150-180 g/meal) initially, gradually increasing to five meals (300-500 g/meal) after two weeks, and was maintained for a total overfeeding period of four weeks.</p><p><strong>Results: </strong>: The results demonstrated that overfeeding significantly increased liver weight and serum lipid levels, accompanied by intracellular lipid droplet accumulation. Concurrently, the downregulation of taurine-conjugated bile acids and the upregulation of free bile acids disrupted cholesterol homeostasis. Crucially, overfeeding triggered gut microbial dysbiosis characterized by Escherichia-Shigella enrichment and norank_o_Clostridia_UCG-014 depletion.</p><p><strong>Conclusion: </strong>: Our work demonstrated that the identification of the \"gut microbiota‒bile acid‒liver axis\" could as a pivotal signaling pathway driving overfeeding-induced foie gras formation while providing a theoretical foundation for overfeeding strategies to mitigate metabolic pathologies in waterfowl production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection index for beef cattle that maximizes overall growth yet constrains birth weight and other traits.","authors":"Kenji Togashi, Toshio Watanabe, Atsushi Ogino, Masakazu Shinomiya, Kazuhito Kurogi, Masanobu Nurimoto","doi":"10.5713/ab.24.0912","DOIUrl":"https://doi.org/10.5713/ab.24.0912","url":null,"abstract":"<p><strong>Objective: </strong>: Maximizing growth throughout the growth period is a practical goal in the beef industry. We developed a novel selection index, called the maximum growth index, that maximizes the growth throughout the growth process and simultaneously achieves the desired weight gain at designated time points by assigning arbitrary values to selection intensity.</p><p><strong>Methods: </strong>: We used a technique called Lagrange multipliers to maximize overall growth throughout the entire growth process and to restrict weight gains to desired amounts at specific times. We developed a selection index by applying random regression (RR) to the growth curve and using the genomically enhanced breeding values (GEBVs) of the RR coefficients as selection index traits.</p><p><strong>Results: </strong>: Examples of the developed index was applied based on assumed data in Japanese Black steers. Selection to maximize growth during the growth period and to moderate birth weight yielded an upwardly convex curve for weight gain during the growth process, and the peak daily gain was greater and earlier than achieved without this selection. Under a selection intensity of 0.5, the index that constrained birth weight to decrease by 2.5 kg achieved the pre-selection final weight 8 weeks earlier than occurred without this selection.</p><p><strong>Conclusion: </strong>: We developed a beef cattle selection index that maximized total weight gain during growth yet constrained birth weight and other traits. The maximum growth index that we developed enables reductions in birth weight concurrent with increases in final weight, thus benefiting the beef industry by increasing final weight and preventing dystocia.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing Precision Livestock Farming: Integrating Artificial Intelligence and Emerging Technologies for Sustainable Livestock Management.","authors":"Luis Orlindo Tedeschi, Pablo Guarnido Lopez, Hector M Menendez Iii, Seongwon Seo","doi":"10.5713/ab.25.0289","DOIUrl":"https://doi.org/10.5713/ab.25.0289","url":null,"abstract":"<p><p>Precision Livestock Farming (PLF) has evolved dramatically from basic monitoring systems to sophisticated artificial intelligence(AI)-driven decision support systems that enhance livestock management efficiency, sustainability, and animal welfare. This review examines the technological evolution of PLF since 2017, highlighting significant advancements in sensing technologies, computer vision, and artificial intelligence. Non-invasive technologies, including RGB-D cameras, 3D imaging systems, and IoT-enabled platforms, now capture detailed biometric and behavioral data in real time, while AI algorithms enable early disease detection, optimize feeding strategies, and improve reproductive management. Integrating these technologies with mechanistic models has created hybrid intelligent frameworks that address longstanding challenges in precision nutrition modeling. Future PLF development will likely focus on integrating large language models, adopting federated learning approaches to address data privacy concerns, and democratizing technologies for small-scale producers. Despite technological progress, challenges remain regarding data standardization, connectivity in rural environments, high implementation costs, and ethical considerations around increased animal monitoring. By fostering interdisciplinary collaboration among animal scientists, engineers, computer scientists, and social scientists, PLF can continue to drive sustainable and efficient practices in livestock production while ensuring that technologies complement rather than replace traditional husbandry knowledge.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of genes and lncRNAs for intramuscular and subcutaneous fat deposition in ducks by transcriptome analysis.","authors":"Tingting Zhou, Xunhao Meng, Wenshuang Liang, Min Xue, Tianqi Yang, Yong Jiang, Hao Bai, Guobin Chang, Guohong Chen, Zhixiu Wang","doi":"10.5713/ab.25.0268","DOIUrl":"https://doi.org/10.5713/ab.25.0268","url":null,"abstract":"<p><p>The purpose of this study was to identify key lncRNAs and mRNAs involved in fat deposition of meat ducks based on whole transcriptome sequencing for intramuscular preadipocyte (IMP-0), intramuscular adipocyte after 4 days of induction (IMP-4), subcutaneous preadipocyte (SCP-0), and subcutaneous adipocyte after 4 days of induction (SCP-4). We found 1419 and 697 differentially expressed genes and lncRNAs in the IMP-0-vs-IMP-4 group, respectively. There were 2307 and 1180 differentially expressed genes and differential lncRNAs, respectively, in the SCP-0-vs-SCP-4 group. The IMP-0-vs-IMP-4 and SCP-0-vs-SCP-4 groups were plotted using a venn diagram to obtain unique differential genes in each group, such as CHKA, PNPLA2, PLPP1, FABP4, ACSL5, UGT8, FAT1 and FADS2. In IMP-0-vs-IMP-4, significant enriched GO terms and KEGG pathways were regulation of MAPK cascade, lipid binding, Arachidonic acid metabolism, etc. In SCP-0-vs-SCP-4, beta-alanine metabolism, Wnt signaling pathway, lipid metabolic process and so on were most significant . Based on correlation analysis, the IMP-0-vs-IMP-4 group constructes a co-expression network of 193 nodes and 275 edges, while the SCP-0-vs-SCP-4 group constitutes a lncRNA-mRNA co-expression network with 564 nodes and 3,471 edges. Key lncRNAs, including MSTRG.8652.4, MSTRG.15586.1, MSTRG.6393.1, XR_217450.4, MSTRG.10341.22, XR_003492841.1, MSTRG.13937.5, and XR_003493886.1 were screened according to connectivity degree. Taken together, the current findings indicated that there are differentially regulated differential genes, lncRNAs, and enrichment pathways in IMP-0-vs-IMP-4 and SCP-0-vs-SCP-4. Because of being differentially regulated, some differential factors were significantly increased in expression in intramuscular adipocyte induction while significantly downregulated in subcutaneous adipocyte induction, such as FABP3, MSTRG.13937.5, and MSTRG.6393.1. Meanwhile, there were also some factors that were specifically regulated, CHKA, PLA2G4A, FADS2, MSTRG.13842.1, MSTRG.16051.2 and MSTRG.13842.1 were significantly downregulated only in subcutaneous adipocytes. This suggests that these lncRNAs and their target genes may play important roles in intramuscular fat and subcutaneous fat deposition.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic transcriptomic landscape of myogenesis in Muscovy ducks (Cairina moschata): Integrative analysis of hub genes post-hatching.","authors":"Xiaofeng Li, Bing Yang","doi":"10.5713/ab.25.0159","DOIUrl":"https://doi.org/10.5713/ab.25.0159","url":null,"abstract":"<p><strong>Objective‌: </strong>‌: Post-hatching myogenesis is a critical determinant of meat yield and quality, with potential regulatory roles of specific genes remaining underexplored‌ in Muscovy ducks (Cairina moschata). This study aimed to identify hub genes governing post-hatching myogenesis through transcriptomic profiling‌.</p><p><strong>Methods‌: </strong>‌: Three White-feathered male Muscovy ducks at 1-day-old (1D) and 80-day-old (80D) were selected, respectively. Following humane euthanasia, the left leg muscles were collected for subsequent RNA extraction, library construction, genome alignment, and transcriptome sequencing. DESeq2 (v1.16.1) was utilized to screen differentially expressed genes (DEGs) based on the criteria of |log2FoldChange| > 1 and P< 0.05 ‌‌. Functional enrichment (Gene Ontology, KEGG, PANTHER) and protein-protein interaction network analyses were conducted via DAVID, KOBAS, PANTHER, and STRING databases, with CytoHubba identifying myogenesis-associated hub genes‌.</p><p><strong>Results: </strong>‌: A total of 1,683 DEGs were identified between 80D and 1D muscles, including 865 upregulated and 818 downregulated genes‌. Totally ten hub genes, such as CD3E, ITK, COL4A6, HADHB, MCCC2, and IVD, were prioritized as key regulators of myogenesis‌. Functional analysis revealed their enrichment in L-leucine catabolic processes and fatty acid β-oxidation‌. Pathway mapping further associated these genes with folate-mediated one-carbon metabolism, glycine/serine/threonine metabolism, and branched-chain amino acid degradation‌.</p><p><strong>Conclusion: </strong>‌: This study delineates a molecular framework for post-hatching myogenesis in Muscovy ducks, highlighting ten hub genes that orchestrate myogenesis through metabolic reprogramming‌. These findings advance genetic strategies for improving poultry meat production and quality‌.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protection of bovine mammary epithelial cells against lipopolysaccharide-induced inflammatory responses using Centella asiatica through its antioxidant and anti-inflammatory activities.","authors":"Do Hyun Kim, Hyuk Cheol Kwon, Jong Hyeon Han, Hyun Su Jung, Dong-Min Shin, Sung Gu Han","doi":"10.5713/ab.25.0089","DOIUrl":"https://doi.org/10.5713/ab.25.0089","url":null,"abstract":"<p><strong>Background: </strong>: Bovine mastitis, an inflammatory condition affecting dairy cow udders, results in decreased milk quantity and quality, posing significant economic losses in the dairy industry. With increasing interest in natural products, Centella asiatica has garnered attention for its potent anti-inflammatory and antioxidant properties. Objective: This study aimed to investigate the potential protective effects of C. asiatica extracts (CE) against lipopolysaccharide (LPS)-induced inflammation in bovine mammary epithelial cells (MAC-T).</p><p><strong>Methods: </strong>: CEs were prepared by extracting C. asiatica leaf powder using ethanol at concentrations of 60%, 70%, 80%, 90%, and 100%. In LPS-stimulated MAC-T cells, the study investigated the ability of CE to reduce oxidative stress and inflammation and its impact on casein protein synthesis.</p><p><strong>Results: </strong>: C. asiatica extract with 60% ethanol (CE60) exhibited the highest radical scavenging activity and total phenolic content among the tested extracts. In MAC-T cells, CE60 significantly attenuated LPS-induced inflammation by down-regulating pro-inflammatory cytokines such as cyclooxygenase-2, tumor necrosis factor-α, interleukin (IL)-1β, and IL-6, as well as inhibiting nuclear factor kappa B activation. CE60 also reduced intracellular reactive oxygen species through upregulating nuclear factor erythroid-2-related factor 2 and associated antioxidant enzymes, including glutathione peroxidase (GPx) 1, GPx4, superoxide dismutase (SOD) 1, SOD2, and catalase. Moreover, CE60 restored the synthesis of casein proteins (CSN1S1, CSN1S2, and CSN2) in LPS-treated MAC-T cells, indicating a protective effect on lactation function under inflammatory conditions.</p><p><strong>Conclusion: </strong>: Taken together, CE60 has potential as a natural substance for the prevention of bovine mastitis by reducing oxidative stress and inflammation in mammary epithelial cells.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary supplementation of Escherichia coli 6-phytase levels on growth performance, nutrient digestibility, and blood inositol in weanling piglets.","authors":"Jong Wan Park, Sang Sik Lee, Abdolreza Hosseindoust, Jun Young Mun, Sang Hun Ha, Habeeb Tajudeen, Priscilla Neves Silvestre, So Dam Choi, Seon Ah Park, Santosh Laxman Ingale, Anushka Lokhande, Jin Soo Kim","doi":"10.5713/ab.25.0186","DOIUrl":"https://doi.org/10.5713/ab.25.0186","url":null,"abstract":"<p><strong>Objective: </strong>: This study aimed to investigate the effect of different levels of phytase supplementation on growth performance parameters, nutrient digestibility, amino acid digestibility, and blood inositol concentration in weanling piglets that fed corn-soybean meal diet.</p><p><strong>Methods: </strong>: Weanling piglets were allocated to one of the five feeding treatments: control, a corn-soybean meal diet; dietary supplementation of 500 FTU/kg phytase; dietary supplementation of 750 FTU/kg phytase; dietary supplementation of 1,000 FTU/kg phytase; dietary supplementation of 1,500 FTU/kg phytase. The experiment had two feeding phases (phase 1, d 1 to 21; phase 2, d 22 to 42) to clarify effects of dietary phytase supplementation.</p><p><strong>Results: </strong>: The final body weight, average daily gain, and gain-to-feed ratio in the whole experimental period were increased linearly in response of elevated levels of dietary phytase supplementation (p < 0.01). There was no significant difference in average daily feed intake in the whole experimental period. In phase 1, there was a linear increase in apparent total tract digestibility of crude protein (CP), gross energy, and calcium as the level of dietary phytase supplementation increases. Phosphorus digestibility tended to be linearly increased (p = 0.056). In phase 2, linear increases were observed (p < 0.01) in calcium and phosphorus digestibility. Additionally, CP digestibility was linearly increased (p < 0.05). Dry matter digestibility showed a quadratic effect (p < 0.05). The digestibility of arginine, leucine, lysine, tryptophan, alanine, and phenylalanine and cysteine showed a linear increase (p < 0.01). There was a linear increase (p < 0.01) in blood myo-inositol levels in phase 2.</p><p><strong>Conclusion: </strong>: Dietary phytase supplementation at levels greater than 1,000 FTU/kg significantly improved growth performance, nutrient digestibility, and blood myo-inositol concentrations in weanling pigs. However, further studies are necessary to investigate the effects of super dosing and to determine the optimal phytase dosage.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of zilpaterol hydrochloride and virginiamycin on growth performance, carcass characteristics, and visceral organ mass in feedlot lambs.","authors":"Mario Alejandro Mejía Delgadillo, Karla Hildeliza Leyva Medina, Jaime Noe Sánchez Pérez, Héctor Aarón Lee Rangel, Hugo de Jesús López Inzunza, Roberto Martínez León, Gamaliel Molina Gámez, José Luis Ponce Covarrubias, Horacio Dávila Ramos, Juan Carlos Robles Estrada","doi":"10.5713/ab.24.0457","DOIUrl":"https://doi.org/10.5713/ab.24.0457","url":null,"abstract":"<p><strong>Objective: </strong>: The objective was to determine the effect of dietary zilpaterol hydrochloride and virginiamycin on growth performance, dietary energetics, carcass characteristics, and visceral organ mass in feedlot lambs.</p><p><strong>Methods: </strong>: Thirty-two crossbred lambs Dorper × Katahdin (34.04±4.23 kg), five months old, were used in a 30 d experiment to evaluate the inclusion of zilpaterol and virginiamycin. Treatments were randomly assigned to pens within blocks, with four replicates per treatment. Data were analyzed as a randomized complete block design arranged as 2 x 2 factorial, with two levels of zilpaterol (0 and 0.20 mg/kg of live weight/d, as hydrochloride form) and two levels of virginiamycin (0 and 22 mg/lamb/d). The diet was based on cracked corn (1.41 Mcal NEg/kg of dry matter and 14.1% of crude protein). Growth performance and dietary energetics variables were recorded. After the feeding trial, lambs were transported to a slaughterhouse for assessment of carcass characteristics, visceral organ mass, and primal cuts.</p><p><strong>Results: </strong>: No interactions were observed between zilpaterol and virginiamycin treatments for most of the evaluated variables, except for the percentage of the empty small intestine. Lambs supplemented with both zilpaterol and virginiamycin showed improvements in live weight, feed efficiency, and energy retention. However, only zilpaterol affected dressing percentage, longissimus thoracis muscle, and body fat reduction. The combined supplementation increased final live weight without altering feed intake, thereby enhancing energy availability to promote carcass weight and dressing carcass compared to lambs treated with zilpaterol alone.</p><p><strong>Conclusion: </strong>: Zilpaterol hydrochloride (0.20mg/kg of live weight/d, equivalent to 7.55 mg/lamb/d) and virginiamycin (22 mg/lamb/d) improved growth and energy retention, but only zilpaterol improved dressing percentage and reduced fat traits of carcass and non-carcass components. The effects of virginiamycin and zilpaterol were found to be cumulative, with better responses in growth and dietary energy components observed in lambs supplemented with both additives.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome and metabolite profiling reveals the mechanism of hepatic lipid metabolism during fasting in chicken.","authors":"Lu Xu, Zhe Liu, Mengling Gong, Zhiheng Wei, Yang Gu, Lisha Yu, Jianfeng Yu, Zhiliang Gu","doi":"10.5713/ab.25.0014","DOIUrl":"https://doi.org/10.5713/ab.25.0014","url":null,"abstract":"<p><strong>Objective: </strong>: Since the liver is key to poultry lipid metabolism and fasting models are widely used in studying animal nutrition metabolism, this study used fasting to explore chicken liver lipid metabolism characteristics, providing a basis for poultry lipid metabolism research.</p><p><strong>Method: </strong>: RNA-seq and metabolomics were combined to analyze 72-hour fasting effects on chicken liver lipid metabolism. Key lipid metabolism-related genes and metabolites were identified, with their mechanisms explored via RNAi and Oil Red O staining.</p><p><strong>Result: </strong>: Metabolomics identified 648 differential metabolites, including 8 (e.g., Arachidonoyl amine) with levels 160-fold higher than controls. Transcriptomics found 849 differentially expressed genes (DEGs), 78 involved in lipid metabolism; PPI analysis revealed hub genes like EHHADH. Transcriptome-metabolome correlation analysis showed 101 DEGs correlated with 99 metabolites, with EHHADH associated with 54 metabolites (the most) and linked to 2-methylcrotonoyl-CoA and 5 pathways via KGML. Fasting upregulated EHHADH, whose overexpression/interference altered mRNA levels of Fabp7, Plin2, ACACA, FASN, PPARα, as well as cholesterol/triglyceride levels. EHHADH overexpression reduced LMH cell lipid deposition, while interference increased it, indicating its role in reducing lipid deposition.</p><p><strong>Conclusion: </strong>: Fasting significantly alters chicken liver lipid metabolism, and EHHADH helps reduce liver lipid deposition.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of rheological properties of pork myofibrillar protein gel and physicochemical and textural properties of low-fat model sausages containing various levels of chickpea powder dried by different methods.","authors":"Min Jae Kim, Koo Bok Chin","doi":"10.5713/ab.25.0164","DOIUrl":"https://doi.org/10.5713/ab.25.0164","url":null,"abstract":"<p><strong>Objective: </strong>: This study evaluated the rheological properties of pork myofibrillar protein (MP) gels, as well as the physicochemical and textural properties of low-fat model sausages (LFMS) formulated with various levels of chickpea powder (CPP) processed via freeze-drying (FCP) or oven-drying (OCP).</p><p><strong>Methods: </strong>: Pork MP gels and LFMS were prepared with varying concentrations (0-1.5%) of CPP, either as FCP or OCP. Viscosity, cooking yield, gel strength, protein surface hydrophobicity, and sulfhydryl group levels were analyzed, in conjunction with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and low-vacuum scanning electron microscopy (LV-SEM) investigations, to evaluate the rheological properties and protein structural changes in MP gels after CPP addition. Additionally, cooking loss, expressible moisture, texture profile, SDS-PAGE, and LV-SEM analyses were conducted to assess the physicochemical and textural properties of LFMS containing CPP. The data were analyzed using one-way and two-way ANOVA, followed by Duncan's multiple range test (p<0.05) to determine significant differences.</p><p><strong>Results: </strong>: Increases in CPP concentration enhanced MP gel viscosity, cooking yield, and gel strength; 1.5% CPP yielded optimal water retention and structural integrity. CPP reduced protein surface hydrophobicity and sulfhydryl content while increasing disulfide bond formation, indicating improved gel network formation. SDS-PAGE confirmed myosin heavy chain reduction and the formation of higher-molecular-weight polymers. In LFMS, CPP reduced cooking loss and expressible moisture; OCP was more effective at higher levels. Texture analysis showed increased hardness and chewiness, whereas cohesiveness and springiness remained unchanged. Microscopy revealed a denser, more uniform structure in sausages containing 1.5% CPP. These changes were correlated with improved water retention and texture.</p><p><strong>Conclusion: </strong>: The findings in this study suggest that CPP, particularly OCP, is a promising functional ingredient for efforts to improve meat product quality by enhancing water retention, gel strength, and texture. CPP also promotes protein polymerization, contributing to a more stable gel network.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}