Animal Bioscience最新文献

{"title":"Change in adipose tissue characteristics and lipid metabolism in natural grazing Mongolian cattle with age.","authors":"Xueting Yu, Lu Chen, Xige He, Yunfei Han, Yajuan Huang, Rina Sha, Gerelt Borjigin","doi":"10.5713/ab.24.0706","DOIUrl":"https://doi.org/10.5713/ab.24.0706","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to elucidate the lipid composition, characteristics, and metabolic mechanisms of subcutaneous adipose tissue (SAT) in natural grazing Mongolian cattle during its development.</p><p><strong>Methods: </strong>The experimental animals consisted of castrated Mongolian cattle, divided into two groups: 10 months old (10 M, n = 12, average weight: 113.26 ± 0.87 kg) and 34 months old (34 M, n = 12, average weight: 390.44 ± 1.23 kg). All animals were selected from the same herd in the Eren Nur grasslands, Xilingol, Inner Mongolia, China. Browning-related genes in SAT were determined using quantitative reverse transcription polymerase chain reaction (RT-qPCR) with 3 replicates for each cattle, and six animals from each group were randomly selected for non-targeted lipidomic techniques to analyze the changes in lipid metabolism with 6 replicates.</p><p><strong>Results: </strong>The yellowness value and relative expression of mitochondrial biogenesis-specific markers, thermogenic markers, uncoupling protein-2 (UCP2), and beige adipocyte marker genes in the SAT increased with age. Multivariate analysis revealed 172 significantly different lipids (SDLs). Triacylglycerols and phosphatidylcholines showed high relative contents. Glycerol and sterol lipid levels were predominantly upregulated during development, whereas phospholipids and sphingolipids were primarily upregulated at 10 M. Developmental time greatly influenced the fatty acid composition of neutral lipids, phospholipids, and free fatty acids. Pathway analysis demonstrated that the 172 SDLs were primarily involved in glycerophospholipid, glycerolipid, ether lipid, and sphingolipid metabolism.</p><p><strong>Conclusion: </strong>The developmental stage significantly affected the lipid quantity, composition, and metabolism of SAT in Mongolian cattle. Certain functional lipids, such as medium-long-chain triglycerides, fatty acid ester of hydroxyl fatty acid, and vitamin D, were detected in the yellow fat of natural grazing Mongolian cattle. These findings provide a comprehensive reference for the metabolic characteristics of yellow fat in Mongolian cattle, contributing to the meat industry chain.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin regulates rabbit sperm motility and kinematics via the MT1/PKC signaling pathway.","authors":"Chongchong Wang, Yanyan Zhang, Shiwen He, Biao Jiang, Jinglei Huang, Hui Peng","doi":"10.5713/ab.24.0593","DOIUrl":"https://doi.org/10.5713/ab.24.0593","url":null,"abstract":"<p><strong>Objective: </strong>Melatonin, a highly conserved molecule, is essential for various physiological functions. Research suggests that incorporating melatonin into semen extender is beneficial to livestock sperm. However, the effect of melatonin on rabbit sperm and the molecular mechanism of melatonin-regulated rabbit sperm motility and kinematics remains unclear. The objective of this study was to reveal the molecular mechanism that melatonin regulates rabbit sperm motility and kinematics.</p><p><strong>Methods: </strong>In this study, we investigated the expression and localization of melatonin-related proteins in rabbit testis and epididymis. Rabbit sperm was incubated under different concentration of melatonin for 60 min or 90 min at 37 °C, and then evaluated sperm motility parameters by IVOS II computer assisted sperm analyzer (CASA) system. Then we detected the sperm plasma membrane integrity, mitochondrial membrane potentials and intracellular reactive oxygen species levels. Furthermore, melatonin receptor antagonists were added to the extender and investigated the involvement of the melatonin receptors in the regulation of sperm motility parameters. Meanwhile, we carried out phosphoproteomics analysis and confirmed the regulation of rabbit sperm kinematics by melatonin via inhibition of the potential signaling pathway.</p><p><strong>Results: </strong>We found that melatonin-related proteins were expressed and localized in rabbit testes and the epididymis. Melatonin (5 mM) markedly increased rabbit sperm motility and kinematics as well as sperm quality. Furthermore, melatonin regulated rabbit sperm motility and kinematics via the MT1 receptor. Meanwhile, phosphoproteomics of rabbit sperm combined with GPS 5.0 software revealed the potential signaling pathway by which melatonin regulates sperm kinematics. Moreover, inhibition of PKC markedly reduced rabbit sperm kinematics, while inhibition of ERK1/2, p38 MAPK, PKG and JNKs with kinase inhibitors did not obviously change sperm kinematics. In addition, inhibition of the MT1 receptor significantly weakened PKC activity, suggesting that PKC is downstream of MT1.</p><p><strong>Conclusion: </strong>We therefore conclude that melatonin regulates rabbit sperm kinematics through the MT1/PKC signaling pathway.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fisetin alleviates oxidative stress and promotes porcine early embryonic development via activation of the NRF2-ARE signalling pathway.","authors":"Huakai Wei, Jiajia Qi, Yanqiu Wang, Hexuan Qu, Chenxuan Yan, Tiantian Li, Yu Wang, Hao Sun, Boxing Sun, Shuang Liang","doi":"10.5713/ab.24.0691","DOIUrl":"https://doi.org/10.5713/ab.24.0691","url":null,"abstract":"<p><strong>Objective: </strong>We improved the developmental capacity of porcine early embryos via supplementation with fisetin during in vitro culture (IVC). In addition, we investigated the antioxidant mechanism of fisetin via activation of the NRF2-ARE signalling pathway in porcine early embryos.</p><p><strong>Methods: </strong>Fisetin (0, 1, 2.5 and 5 μM) was supplemented during IVC to observe its effects on the developmental ability of porcine parthenogenetic activation (PA), in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) embryos. The effects of fisetin supplementation on the antioxidant capacity, mitochondrial function, cell proliferation and apoptosis levels of porcine PA embryos were detected via fluorescence staining, and the expression levels of genes related to apoptosis, pluripotency and the NRF2 pathway were also examined.</p><p><strong>Results: </strong>Compared with the control, 1 μM fisetin during IVC increased the developmental ability of porcine PA, IVF and SCNT embryos. Additionally, fisetin significantly decreased reactive oxygen species (ROS) and apoptosis levels; increased pluripotency during embryonic development, cell proliferation and glutathione (GSH) levels; and improved mitochondrial function in PA embryos. Moreover, the levels of Kelch-like ECH-associated protein 1 (KEAP1) significantly decreased, and the levels of NFE2-like bZIP transcription factor 2 (NRF2) and its downstream antioxidant enzymes significantly increased after fisetin supplementation.</p><p><strong>Conclusion: </strong>Our data reveal that fisetin protects porcine early embryos from oxidative stress during IVC by activating the NRF2-ARE signalling pathway, thereby improving the success of in vitro embryo production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Brix values for estimating nitrogen and gross energy concentrations of pig urine.","authors":"Jeonghyeon Son, Hyunseok Do, Beob Gyun Kim","doi":"10.5713/ab.24.0684","DOIUrl":"https://doi.org/10.5713/ab.24.0684","url":null,"abstract":"<p><p>The present study aimed to develop equations for estimating the dry matter (DM), nitrogen (N), and gross energy (GE) concentrations in pig urine using Brix values. A total of 96 urine samples were obtained from pigs of 6.0 to 62.2 kg initial body weight fed 12 diets in three experiments. The animals were fed a corn-soybean meal-based diet or a corn diet. The N concentrations in the urine samples ranged from 0.12 to 2.06% and the GE concentrations ranged from 2 to 187 kcal/kg. The urine Brix values were positively correlated with urinary DM, N, and GE concentrations (r = 0.94, 0.87, and 0.93, respectively; p<0.001). The models for estimating urinary DM, N, and GE concentrations were: urinary DM (%) = 0.590×Brix + 0.315 (r2 = 0.91; p<0.001); urinary N (%) = 0.0798×Brix + 0.148 (r2 = 0.84; p<0.001); urinary GE (kcal/kg) = 11.96×Brix + 2.33 (r2 = 0.87; p<0.001), where the Brix value of urine is expressed as %. Overall, the Brix values correlated positively with the DM, N, and GE concentrations in pig urine and can thus be used for estimating the concentrations of these urinary components.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"circRNA18_46222157_46248185 inhibits melanogenesis by targeting miR-211/EP300 pathway in goat melanocytes.","authors":"Kai Yuan Ji, Xue Qing Zhang, Yi Wei Zhao, Chun E Liang, Xin Yuan, Yun Hai Zhang","doi":"10.5713/ab.24.0316","DOIUrl":"10.5713/ab.24.0316","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the effects of circRNA18_46222157_46248185 (named circRNA18) on goat melanogenesis, which differs significantly in goat skins isolated from white and brown coat-colored skins.</p><p><strong>Methods: </strong>Expression patterns of circRNA18 in goat skin and melanocytes were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and in situ hybridization. The circRNA18 interference vector was designed and synthesized to transfect melanocytes and detect the effect of circRNA18 interference on melanin production. Bioinformatics software was used to predict the targeted adsorption miRNAs of circRNA18, verified by luciferase assay. A miRNA expression vector was constructed and transfected into melanocytes to detect the effect of miRNA on melanin production, and the targeted regulatory genes were detected by luciferase assay. Target gene interference vector was constructed to detect the influence of target gene interference on melanin production.</p><p><strong>Results: </strong>qRT-PCR results unveiled distinct expression patterns of circRNA18 in diverse tissues of male and female goats, while in situ hybridization assays showed that circRNA18 is expressed in the cytoplasm of melanocytes. Functional analysis demonstrated that the downregulation of circRNA18 in melanocytes leads to a significant increase (p<0.01) in melanin production. Bioinformatics analysis identified a potential miR-211 binding site on circRNA18, and luciferase assay confirmed their interaction. Overexpression of miR-211 in melanocytes significantly augmented (p<0.01) melanin production. There were two potential miR-211 binding sites on adenoviral E1A-binding protein (EP300), and the overexpression of miR-211 in melanocytes significantly decreased (p<0.001) EP300 expression, with luciferase assay confirming their interaction. Downregulation of EP300 expression in melanocytes through siRNA-EP300 transfection results in a substantial increase (p<0.05) in melanin production. qRT-PCR results indicated that overexpression of mimics-circRNA18 in melanocytes markedly suppressed (p<0.0001) miR-211 expression, significantly elevated (p<0.01) EP300 expression, and significantly inhibited (p<0.001) melanin production.</p><p><strong>Conclusion: </strong>circRNA18_46222157_46248185 acted as a negative regulator of melanogenesis in goat melanocytes by targeting the miR-211/EP300 pathway, and guiding animal hair color breeding strategies.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"255-266"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of immune-associated genes with altered expression in the spleen of mice enriched with probiotic Lactobacillus species using RNA-seq profiling.","authors":"Anh Duc Truong, Ha Thi Thanh Tran, Nhu Thi Chu, Lanh Phan, Hoai Thi Phan, Thu Huong Dang, Hoang Vu Dang, La Anh Nguyen","doi":"10.5713/ab.24.0280","DOIUrl":"10.5713/ab.24.0280","url":null,"abstract":"<p><strong>Objective: </strong>Probiotics are living microorganisms that can provide health benefits when consumed. Here, we investigated the effects of probiotics on gene expression in the spleen of mice using RNA-sequencing analysis between negative control and probiotic groups (including 4 Lactobacillus strains: Lactobacillus fermentum, L. casei, L. plantarum, and L. brevis).</p><p><strong>Methods: </strong>Mice exposed with probiotic in 4 weeks by intragastric administration. Then, spleen tissues of the control and probiotics groups were collected on days 14 and 28 for RNA sequencing.</p><p><strong>Results: </strong>In total, 665, 186, and 81 differentially expressed genes (DEGs) were significantly expressed on day 14 vs control, day 28 vs control groups, and probiotics day 28 vs day 14 groups, respectively. On the other hand, 12 toll-like receptor genes underwent additional validation through quantitative real-time polymerase chain reaction (qRT-PCR), affirming the increased alignment between qRT-PCR and RNA-Seq findings. In addition, the Kyoto encyclopedia of genes and genomes and gene ontology analyses revealed that the DEGs were predominantly enriched in defense responses to pathogens, including inflammatory bowel diseases, malaria, leukaemia virus 1, and herpes virus, as well as immune processes related to immune response and signal transduction. This study represents the first investigation into mice's gene expression in the spleen exposed to probiotics using Lactobacillus spp. isolated from a field strain in Vietnam.</p><p><strong>Conclusion: </strong>Our results provide valuable insights into the impacts and functions of probiotics on mammalian development, offering crucial information for the potential therapeutic use of probiotics in defending against pathogens in Vietnam. The findings from this study highlight the potential of probiotics in modulating gene expression in the spleen, which may have implications for immune function and overall health in mice.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"336-349"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary effects of protected fat, soybean meal, and heat-treated soybean meal on performance, physiological parameters, and behavioral measurements of early-fattening Hanwoo steers under heat stress conditions.","authors":"Jun Sik Woo, Sun Sik Jang, Jeong Hoon Kim, Hong Gu Lee, Keun Kyu Park","doi":"10.5713/ab.24.0236","DOIUrl":"10.5713/ab.24.0236","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the effects of increased levels of dietary total digestible nutrient (TDN) and crude protein (CP) using protected fat (PF), soybean meal (SB), and heat-treated soybean meal (HSB) on performance, physiological parameters, and behavioral measurements of early-fattening Hanwoo steers under heat stress conditions.</p><p><strong>Methods: </strong>Thirty-six steers (480.9±58.6 kg, 15.9±1.4 months) were assigned to 4 treatments: control (TDN 75%, CP 15%, rumen degradable protein [RDP]:rumen undegradable protein [RUP] = 62:48); PF (TDN 82.5%, CP 15%, RDP:RUP = 62:48); PF+SB (TDN 82.5%, CP 16.5%, RDP:RUP = 62:48); and PF+SB+HSB (TDN 82.5%, CP 16.5%, RDP:RUP = 48:52) for a total of 16 weeks with division of 4 weeks. The average temperature-humidity index (THI) was 82.9 (1st; moderate), 76.9 (2nd; mild), 70.9 (3rd; comfort), and 65.8 (4th period; comfort).</p><p><strong>Results: </strong>Dry matter intake during whole period did not differ among treatments but decreased by 34% and 19%, respectively during 1st and 2nd compared to 4th. Average daily gain (ADG) of PF+SB+HSB was higher (p<0.05) than that of control during the 1st period, and those of both PF+SB and PF+SB+HSB were higher (p<0.05) than Control during the 2nd. The ADG during whole period was in the order of PF+SB+HSB (1.23), PF+SB (1.18), PF (1.11), control (0.98 kg/d) (p<0.05). As THI increased, rectal temperature and all blood parameters increased, while blood glucose levels decreased (p<0.05). Behavioral changes during 1st period compared to 3rd included decreases in lying (24%), walking (48%), and eating (40%), and increases in total standing (50%) and drinking (43%) (p<0.05). Rumination during standing was 38% higher, and rumination during lying was 32% lower (p<0.05).</p><p><strong>Conclusion: </strong>This study demonstrates 10% of increased levels of dietary TDN using PF and CP considering RUP can prevent performance reduction in early-fattening of heatstressed Hanwoo steers and have positive effects on performance recovery from post-heat stress.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"278-292"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary supplementation of xylanase alone or combination of xylanase and β-glucanase on growth performance, meat quality, intestinal measurements, and nutrient utilization in broiler chickens.","authors":"Deok Yun Kim, Kang Hyeon Kim, Eun Cheol Lee, Ju Kyoung Oh, Min Ah Park, Dong Yong Kil","doi":"10.5713/ab.24.0430","DOIUrl":"10.5713/ab.24.0430","url":null,"abstract":"<p><strong>Objective: </strong>The current study aimed to investigate the effect of dietary supplementation of xylanase alone or combination of xylanase and β-glucanase in high non-starch polysaccharides (NSP) diets with low energy on growth performance, meat quality, intestinal measurements, stress responses, and energy and nutrient utilization in broiler chickens.</p><p><strong>Methods: </strong>A total of four hundred 8-d-old Ross 308 broiler chickens were randomly allotted to 1 of 4 treatment groups with 10 replicates. A positive control (PC) diet was formulated with adequate energy and nutrients, whereas a negative control (NC) diet had 100 kcal/kg less nitrogen-corrected apparent metabolizable energy than the PC diet with increasing inclusion of high NSP ingredients. Two additional diets were produced by supplementing 0.1% xylanase alone or 0.1% xylanase and β-glucanase mixture in the NC diet. Experiments lasted for 27 d.</p><p><strong>Results: </strong>Birds fed PC diets had less (p<0.05) feed conversion ratio (FCR) than those fed NC diets. Birds fed NC diets supplemented with xylanase alone or combination of xylanase and β-glucanase had less (p<0.05) FCR than those fed NC diets. Dietary supplementation of xylanase alone in NC diets exhibited the highest (p<0.05) breast meat pH among dietary treatments. Birds fed PC diets or NC diets supplemented with xylanase and β-glucanase combination exhibited greater (p<0.05) villus height:crypt depth ratio than those fed NC diets. Dietary supplementation of xylanase alone and combination of xylanase and β-glucanase in NC diets decreased (p<0.05) ileal digesta viscosity and increased (p<0.05) xylo-oligosaccharide concentrations in the gastrointestinal tract (GIT) compared with NC diets without affecting energy and nutrient utilization in NC diets.</p><p><strong>Conclusion: </strong>Dietary supplementation of xylanase in high NSP diets with low energy concentrations improves growth performance by decreasing digesta viscosity and increasing concentrations of xylo-oligosaccharides in the GIT of broiler chickens. However, little additional benefits of β-glucanase supplementation in combination with xylanase are identified for broiler chickens.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"325-335"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel p.127Val>Ile single nucleotide polymorphism in the MTNR1A gene and its relation to litter size in Thin-tailed Indonesian ewes.","authors":"Mutasem Abuzahra, Mohammed Baqur S Al-Shuhaib, Dwi Wijayanti, Mustofa Helmi Effendi, Imam Mustofa, Ikechukwu Benjamin Moses","doi":"10.5713/ab.24.0187","DOIUrl":"10.5713/ab.24.0187","url":null,"abstract":"<p><strong>Objective: </strong>The primary objective was to identify and characterize the single nucleotide polymorphisms (SNPs) within the MTNR1A gene sequence in Thin-tailed Indonesian ewes to assess the possible association of MTNR1A gene polymorphism with litter size trait.</p><p><strong>Methods: </strong>Forty-seven Thin-tailed Indonesian sheep were selected for the study. Genotyping involved collecting blood samples, and sequencing exon 2 of the MTNR1A gene.</p><p><strong>Results: </strong>The study identified 19 novel SNPs, with 10 being non-synonymous variations, in the MTNR1A gene of Thin-tailed Indonesian ewes. One non-synonymous SNP (rs1087815963) showed a significant association with litter size, with the GC genotype exhibiting a higher average litter size than the GG genotype. The deleterious impact of p.Val127Ile SNP was predicted by various in silico tools that predicted a highly damaging effect of p.Val127Ile SNP on the structure, function, and stability of MTNR1A. Docking reactions showed a critical involvement of this locus with the binding with melatonin.</p><p><strong>Conclusion: </strong>In conclusion, the results of our study suggest that rs1087815963 has a remarkable negative impact on the MTNR1A with a putative alteration in the binding with melatonin. Therefore, the implementation of the novel p.Val127Ile could be a useful marker in marker-assisted selection.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"209-222"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth performance of pigs fed low-protein diets supplemented with crystalline amino acids at different growth stages.","authors":"Inho Cho, Changsu Kong","doi":"10.5713/ab.24.0339","DOIUrl":"10.5713/ab.24.0339","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the impact of reducing dietary crude protein (CP) coupled with supplementation of indispensable amino acids (AA) on growth performance of pigs at different growth stages.</p><p><strong>Methods: </strong>A total of 126 (63 barrows and 63 gilts), 90 (45 barrows and gilts), and 72 (36 barrows and 36 gilts) pigs with average weights of 9.8±1.62, 30.6±2.31, and 58.3±2.95 kg in the nursery, growing, and finishing stages, respectively, were assigned to three dietary treatments with six replicates in a randomized complete block design. The pigs had ad libitum access to water and fed three experimental diets, each supplemented with all indispensable AA and subjected to a 2% reduction in CP from the upper limits of 18%, 16%, and 16% established for the nursery, growing, and finishing stages, respectively.</p><p><strong>Results: </strong>In the nursery stage, from 0 to 2 weeks, reducing dietary CP concentrations decreased average daily feed intake (ADFI; linear, p = 0.04). From 2 to 4 weeks, dietary CP reduction decreased average daily gain (ADG; linear, p<0.01; quadratic, p = 0.02), ADFI (linear, p = 0.04), and gain-to-feed ratio (G:F; linear, p = 0.01). From 0 to 4 weeks, reduction in dietary CP concentrations decreased ADG (linear p<0.01), and G:F (linear, p = 0.01). In the growing stage, the dietary CP reduction did not affect growth performance. During the finishing stage, decrease in dietary CP concentrations decreased ADFI from 3 to 6 weeks (quadratic, p<0.01) and 0 to 6 weeks (quadratic, p = 0.01).</p><p><strong>Conclusion: </strong>Dietary CP reduction with indispensable AA supplementation potentially decreases the growth performance of nursery pigs but may not decrease the growth performance of growing and finishing pigs.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"316-324"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}