Animal Bioscience最新文献

{"title":"Protection of bovine mammary epithelial cells against lipopolysaccharide-induced inflammatory responses using Centella asiatica through its antioxidant and anti-inflammatory activities.","authors":"Do Hyun Kim, Hyuk Cheol Kwon, Jong Hyeon Han, Hyun Su Jung, Dong-Min Shin, Sung Gu Han","doi":"10.5713/ab.25.0089","DOIUrl":"https://doi.org/10.5713/ab.25.0089","url":null,"abstract":"<p><strong>Background: </strong>: Bovine mastitis, an inflammatory condition affecting dairy cow udders, results in decreased milk quantity and quality, posing significant economic losses in the dairy industry. With increasing interest in natural products, Centella asiatica has garnered attention for its potent anti-inflammatory and antioxidant properties. Objective: This study aimed to investigate the potential protective effects of C. asiatica extracts (CE) against lipopolysaccharide (LPS)-induced inflammation in bovine mammary epithelial cells (MAC-T).</p><p><strong>Methods: </strong>: CEs were prepared by extracting C. asiatica leaf powder using ethanol at concentrations of 60%, 70%, 80%, 90%, and 100%. In LPS-stimulated MAC-T cells, the study investigated the ability of CE to reduce oxidative stress and inflammation and its impact on casein protein synthesis.</p><p><strong>Results: </strong>: C. asiatica extract with 60% ethanol (CE60) exhibited the highest radical scavenging activity and total phenolic content among the tested extracts. In MAC-T cells, CE60 significantly attenuated LPS-induced inflammation by down-regulating pro-inflammatory cytokines such as cyclooxygenase-2, tumor necrosis factor-α, interleukin (IL)-1β, and IL-6, as well as inhibiting nuclear factor kappa B activation. CE60 also reduced intracellular reactive oxygen species through upregulating nuclear factor erythroid-2-related factor 2 and associated antioxidant enzymes, including glutathione peroxidase (GPx) 1, GPx4, superoxide dismutase (SOD) 1, SOD2, and catalase. Moreover, CE60 restored the synthesis of casein proteins (CSN1S1, CSN1S2, and CSN2) in LPS-treated MAC-T cells, indicating a protective effect on lactation function under inflammatory conditions.</p><p><strong>Conclusion: </strong>: Taken together, CE60 has potential as a natural substance for the prevention of bovine mastitis by reducing oxidative stress and inflammation in mammary epithelial cells.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary supplementation of Escherichia coli 6-phytase levels on growth performance, nutrient digestibility, and blood inositol in weanling piglets.","authors":"Jong Wan Park, Sang Sik Lee, Abdolreza Hosseindoust, Jun Young Mun, Sang Hun Ha, Habeeb Tajudeen, Priscilla Neves Silvestre, So Dam Choi, Seon Ah Park, Santosh Laxman Ingale, Anushka Lokhande, Jin Soo Kim","doi":"10.5713/ab.25.0186","DOIUrl":"https://doi.org/10.5713/ab.25.0186","url":null,"abstract":"<p><strong>Objective: </strong>: This study aimed to investigate the effect of different levels of phytase supplementation on growth performance parameters, nutrient digestibility, amino acid digestibility, and blood inositol concentration in weanling piglets that fed corn-soybean meal diet.</p><p><strong>Methods: </strong>: Weanling piglets were allocated to one of the five feeding treatments: control, a corn-soybean meal diet; dietary supplementation of 500 FTU/kg phytase; dietary supplementation of 750 FTU/kg phytase; dietary supplementation of 1,000 FTU/kg phytase; dietary supplementation of 1,500 FTU/kg phytase. The experiment had two feeding phases (phase 1, d 1 to 21; phase 2, d 22 to 42) to clarify effects of dietary phytase supplementation.</p><p><strong>Results: </strong>: The final body weight, average daily gain, and gain-to-feed ratio in the whole experimental period were increased linearly in response of elevated levels of dietary phytase supplementation (p < 0.01). There was no significant difference in average daily feed intake in the whole experimental period. In phase 1, there was a linear increase in apparent total tract digestibility of crude protein (CP), gross energy, and calcium as the level of dietary phytase supplementation increases. Phosphorus digestibility tended to be linearly increased (p = 0.056). In phase 2, linear increases were observed (p < 0.01) in calcium and phosphorus digestibility. Additionally, CP digestibility was linearly increased (p < 0.05). Dry matter digestibility showed a quadratic effect (p < 0.05). The digestibility of arginine, leucine, lysine, tryptophan, alanine, and phenylalanine and cysteine showed a linear increase (p < 0.01). There was a linear increase (p < 0.01) in blood myo-inositol levels in phase 2.</p><p><strong>Conclusion: </strong>: Dietary phytase supplementation at levels greater than 1,000 FTU/kg significantly improved growth performance, nutrient digestibility, and blood myo-inositol concentrations in weanling pigs. However, further studies are necessary to investigate the effects of super dosing and to determine the optimal phytase dosage.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of zilpaterol hydrochloride and virginiamycin on growth performance, carcass characteristics, and visceral organ mass in feedlot lambs.","authors":"Mario Alejandro Mejía Delgadillo, Karla Hildeliza Leyva Medina, Jaime Noe Sánchez Pérez, Héctor Aarón Lee Rangel, Hugo de Jesús López Inzunza, Roberto Martínez León, Gamaliel Molina Gámez, José Luis Ponce Covarrubias, Horacio Dávila Ramos, Juan Carlos Robles Estrada","doi":"10.5713/ab.24.0457","DOIUrl":"https://doi.org/10.5713/ab.24.0457","url":null,"abstract":"<p><strong>Objective: </strong>: The objective was to determine the effect of dietary zilpaterol hydrochloride and virginiamycin on growth performance, dietary energetics, carcass characteristics, and visceral organ mass in feedlot lambs.</p><p><strong>Methods: </strong>: Thirty-two crossbred lambs Dorper × Katahdin (34.04±4.23 kg), five months old, were used in a 30 d experiment to evaluate the inclusion of zilpaterol and virginiamycin. Treatments were randomly assigned to pens within blocks, with four replicates per treatment. Data were analyzed as a randomized complete block design arranged as 2 x 2 factorial, with two levels of zilpaterol (0 and 0.20 mg/kg of live weight/d, as hydrochloride form) and two levels of virginiamycin (0 and 22 mg/lamb/d). The diet was based on cracked corn (1.41 Mcal NEg/kg of dry matter and 14.1% of crude protein). Growth performance and dietary energetics variables were recorded. After the feeding trial, lambs were transported to a slaughterhouse for assessment of carcass characteristics, visceral organ mass, and primal cuts.</p><p><strong>Results: </strong>: No interactions were observed between zilpaterol and virginiamycin treatments for most of the evaluated variables, except for the percentage of the empty small intestine. Lambs supplemented with both zilpaterol and virginiamycin showed improvements in live weight, feed efficiency, and energy retention. However, only zilpaterol affected dressing percentage, longissimus thoracis muscle, and body fat reduction. The combined supplementation increased final live weight without altering feed intake, thereby enhancing energy availability to promote carcass weight and dressing carcass compared to lambs treated with zilpaterol alone.</p><p><strong>Conclusion: </strong>: Zilpaterol hydrochloride (0.20mg/kg of live weight/d, equivalent to 7.55 mg/lamb/d) and virginiamycin (22 mg/lamb/d) improved growth and energy retention, but only zilpaterol improved dressing percentage and reduced fat traits of carcass and non-carcass components. The effects of virginiamycin and zilpaterol were found to be cumulative, with better responses in growth and dietary energy components observed in lambs supplemented with both additives.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome and metabolite profiling reveals the mechanism of hepatic lipid metabolism during fasting in chicken.","authors":"Lu Xu, Zhe Liu, Mengling Gong, Zhiheng Wei, Yang Gu, Lisha Yu, Jianfeng Yu, Zhiliang Gu","doi":"10.5713/ab.25.0014","DOIUrl":"https://doi.org/10.5713/ab.25.0014","url":null,"abstract":"<p><strong>Objective: </strong>: Since the liver is key to poultry lipid metabolism and fasting models are widely used in studying animal nutrition metabolism, this study used fasting to explore chicken liver lipid metabolism characteristics, providing a basis for poultry lipid metabolism research.</p><p><strong>Method: </strong>: RNA-seq and metabolomics were combined to analyze 72-hour fasting effects on chicken liver lipid metabolism. Key lipid metabolism-related genes and metabolites were identified, with their mechanisms explored via RNAi and Oil Red O staining.</p><p><strong>Result: </strong>: Metabolomics identified 648 differential metabolites, including 8 (e.g., Arachidonoyl amine) with levels 160-fold higher than controls. Transcriptomics found 849 differentially expressed genes (DEGs), 78 involved in lipid metabolism; PPI analysis revealed hub genes like EHHADH. Transcriptome-metabolome correlation analysis showed 101 DEGs correlated with 99 metabolites, with EHHADH associated with 54 metabolites (the most) and linked to 2-methylcrotonoyl-CoA and 5 pathways via KGML. Fasting upregulated EHHADH, whose overexpression/interference altered mRNA levels of Fabp7, Plin2, ACACA, FASN, PPARα, as well as cholesterol/triglyceride levels. EHHADH overexpression reduced LMH cell lipid deposition, while interference increased it, indicating its role in reducing lipid deposition.</p><p><strong>Conclusion: </strong>: Fasting significantly alters chicken liver lipid metabolism, and EHHADH helps reduce liver lipid deposition.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of rheological properties of pork myofibrillar protein gel and physicochemical and textural properties of low-fat model sausages containing various levels of chickpea powder dried by different methods.","authors":"Min Jae Kim, Koo Bok Chin","doi":"10.5713/ab.25.0164","DOIUrl":"https://doi.org/10.5713/ab.25.0164","url":null,"abstract":"<p><strong>Objective: </strong>: This study evaluated the rheological properties of pork myofibrillar protein (MP) gels, as well as the physicochemical and textural properties of low-fat model sausages (LFMS) formulated with various levels of chickpea powder (CPP) processed via freeze-drying (FCP) or oven-drying (OCP).</p><p><strong>Methods: </strong>: Pork MP gels and LFMS were prepared with varying concentrations (0-1.5%) of CPP, either as FCP or OCP. Viscosity, cooking yield, gel strength, protein surface hydrophobicity, and sulfhydryl group levels were analyzed, in conjunction with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and low-vacuum scanning electron microscopy (LV-SEM) investigations, to evaluate the rheological properties and protein structural changes in MP gels after CPP addition. Additionally, cooking loss, expressible moisture, texture profile, SDS-PAGE, and LV-SEM analyses were conducted to assess the physicochemical and textural properties of LFMS containing CPP. The data were analyzed using one-way and two-way ANOVA, followed by Duncan's multiple range test (p<0.05) to determine significant differences.</p><p><strong>Results: </strong>: Increases in CPP concentration enhanced MP gel viscosity, cooking yield, and gel strength; 1.5% CPP yielded optimal water retention and structural integrity. CPP reduced protein surface hydrophobicity and sulfhydryl content while increasing disulfide bond formation, indicating improved gel network formation. SDS-PAGE confirmed myosin heavy chain reduction and the formation of higher-molecular-weight polymers. In LFMS, CPP reduced cooking loss and expressible moisture; OCP was more effective at higher levels. Texture analysis showed increased hardness and chewiness, whereas cohesiveness and springiness remained unchanged. Microscopy revealed a denser, more uniform structure in sausages containing 1.5% CPP. These changes were correlated with improved water retention and texture.</p><p><strong>Conclusion: </strong>: The findings in this study suggest that CPP, particularly OCP, is a promising functional ingredient for efforts to improve meat product quality by enhancing water retention, gel strength, and texture. CPP also promotes protein polymerization, contributing to a more stable gel network.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular regulatory mechanisms of dietary supplementation with Allium mongolicum Regel powder to improve muscle development and meat quality in Angus calves.","authors":"Wangjing Liu, Chenxu Sun, Huixia Gao, Jianjian He, Aihuan Yu, Yaodi Xie, Haibo Yao, Jiang Hu, Zhaomin Lei","doi":"10.5713/ab.24.0809","DOIUrl":"10.5713/ab.24.0809","url":null,"abstract":"<p><strong>Objective: </strong>Supplementing animal feed with Allium mongolicum Regel powder (AMRP) additives can promote muscle production and improve meat quality. Here, we explored the effects of dietary AMRP supplementation on the performance, meat quality, and muscle transcriptome profile of Angus calves.</p><p><strong>Methods: </strong>Twelve healthy female black Angus calves (average body weight = 280.4±15.74 kg, average age = 14±0.6 months) of the same genetic background were randomly assigned to two feed groups: control (CON; basal diet without any supplementation) and AMRP (basal diet supplemented with 20 g of AMRP per calf per day).</p><p><strong>Results: </strong>In general, compared with the control group, dietary AMRP supplementation significantly increased the longissimus thoracis muscle area (p = 0.027) and pH24h (p = 0.027) but significantly reduced Warner-Bratzler shear force (p = 0.009) and cooking loss (p<0.001). Moreover, 1,284 differentially expressed genes (DEGs) were identified in AMRP-supplemented Angus calves. Pathway analysis revealed that the DEGs were involved in multiple pathways related to muscle development and fat deposition, such as the focal adhesion and MAPK pathways.</p><p><strong>Conclusion: </strong>Dietary supplementation of AMRP improved muscle growth and development in Angus beef cattle. It also significantly modulated meat quality, possibly altering signaling pathways by influencing key gene expression. Our results provide novel insights into the development of the meat industry and indicate the mechanism through which AMRP regulates muscle development and improves meat quality at the molecular level.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1798-1816"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of bacteriophage cocktail supplementation in gestation diet on reproductive performance, blood profile, milk composition, and fecal microflora of sows.","authors":"Jinsu Hong, Geon Il Lee, Jae-Cheol Jang, Yoo Yong Kim","doi":"10.5713/ab.24.0796","DOIUrl":"10.5713/ab.24.0796","url":null,"abstract":"<p><strong>Objective: </strong>The present study aimed to evaluate the effects of bacteriophage cocktail supplementation in diet of gestation sows on both the sows and their progeny.</p><p><strong>Methods: </strong>A total of 57 F1 multiparous sows (Yorkshire×Landrace) were allotted to one of three treatment groups in a completely randomized design. The sows were fed cornsoybean meal-based diet supplemented with 0%, 0.05%, or 0.10% of a bacteriophage cocktail during the gestation, followed by a common diet during lactation. Body weight and backfat thickness were measured during the trial along with blood collection for immunoglobulin analysis. Fecal samples were collected on 4, 6, 8, and 10 weeks of gestation period to examine fecal microflora. Litter performance and milk composition were investigated at 24 hrs postpartum and on d 21 of lactation.</p><p><strong>Results: </strong>Increasing bacteriophage cocktail supplementation levels in gestation diets resulted in linear increas (p<0.05) in feed intake of lactating sows and in the weight of live litters, while showing quadratic increas (p<0.05) in litter weight gain from d 0 to 21 of lactation. Dietary treatment had no impact on the serum concentrations of IgG and IgM in the sows. Increasing dietary bacteriophage cocktail levels in gestation diets resulted in a linear increas (p<0.05) in the fat content of colostrum. The elevation of dietary bacteriophage cocktail in microflora of sows resluted in a significant increase (p<0.05) in Lactobacillus count for d 63 and 105 of the gestation period, while concurrently decreasing Escherichia coli (E. coli; p<0.05) and Salmonella (p<0.10) counts during the late gestation period.</p><p><strong>Conclusion: </strong>The supplementation of a 0.05% bacteriophage cocktail in the diet of gestating sows could improve total litter weight, alive litter weight at birth, and litter weight gain during the lactation. This effect is attributed to positive changes in fecal microflora with an increase of Lactobacillus and a decrease of E. coli and Salmonella.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1708-1718"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serotonin promotes calcium accumulation and inhibits lipid accumulation in cultured goat mammary epithelial cells through HTR2A.","authors":"ZhiFei Zhang, BinHan Li, YingFei Wang, Tumaresi Abuduwufuer, Kang Hu, HuiLing Zheng","doi":"10.5713/ab.24.0792","DOIUrl":"10.5713/ab.24.0792","url":null,"abstract":"<p><strong>Objective: </strong>Fatty acids and calcium are both important nutrients in goat milk. Investigating the upstream molecular regulatory mechanisms that control the synthesis of milk fat and milk calcium in the mammary gland can help improve the quality of milk at its source. The objective of this study was to investigate the effects and regulatory pathways of serotonin (5-hydroxytryptamine, 5-HT) and its receptors on lipid synthesis and calcium ion levels in goat mammary epithelial cells (GMECs).</p><p><strong>Methods: </strong>GMECs isolated from live goats were treated with serotonin, overexpression of Serotonin receptor 2A (HTR2A), Sarpogrelate ([SAR] the specific antagonist of HTR2A), or a combination of these agents. The expression of genes related to de novo lipid synthesis in GMECs were detected using the Quantitative Real-Time polymerase chain reaction, the content of lipid droplets was detected using the BODIPY assay, and the calcium content was detected using the calcium chelating probe Fluo-3AM assay.</p><p><strong>Results: </strong>5-HT dose-dependently promotes the activity of GEMCs, significantly inhibits the mRNA expression of key genes involved in de novo lipid synthesis such as ACC, FASN, SREBP1, SCD1 and ELOVL6 at a concentration of 100 μM, reduces triglyceride and total cholesterol content, suppresses lipid droplet accumulation in cells, and simultaneously promotes calcium accumulation in cells. Furthermore, overexpression of HTR2A in GMECs also induces an increase in cellular calcium levels and inhibits lipid synthesis and accumulation in cells. However, treatment of cells with SAR, the specific antagonist of HTR2A, significantly increases the levels of triglycerides, total cholesterol, and lipid droplet accumulation in cells.</p><p><strong>Conclusion: </strong>5-HT inhibits lipid synthesis in GMECs while promoting an increase in cellular calcium levels, and this effect is mediated by the HTR2A receptor. Furthermorey, antagonists targeting HTR2A can reverse the inhibition of lipid synthesis and accumulation in cells.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1633-1643"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of milk fortification with an advanced chelated trace minerals supplement on growth, health, and nutrient digestibility in dairy calves.","authors":"Hossein Rajaei-Sharifabadi, Hadi Rahmani, Zahra Shokri, Saideh Fakharzadeh, Somayeh Kalanaky, Mohammad Hassan Nazaran, Ahmad Reza Seradj","doi":"10.5713/ab.24.0701","DOIUrl":"10.5713/ab.24.0701","url":null,"abstract":"<p><strong>Objective: </strong>Recent advancements in chelate compound technology offer improved bioavailability of trace minerals, potentially enhancing growth performance and reducing disease incidence. Milk fortification is an efficient method to supplement young calves before transition to solid feed. This study evaluated the response of dairy calves to milk fortification with an advanced chelated trace minerals supplement containing Zn, Mn, Cu, Fe, Co, Cr, and Se, each selected for their specific roles in calf development, synthesized using chelate compoundtechnology.</p><p><strong>Methods: </strong>Forty-two Holstein dairy calves (21 males and 21 females) were randomly assigned to three experimental groups: control (CON, unfortified milk), chelated minerals (BON, milk fortified with chelated trace minerals), and inorganic minerals (IOR, milk fortified with inorganic trace minerals). Calves received their respective milk treatments daily from d 3 until weaning at 70 d of age. Growth performance and body measurements were assessed throughout the experiment, while rectal temperature, fecal consistency, and frequency of medical treatment were recorded until d 21. Blood metabolites and nutrient digestibility were also determined.</p><p><strong>Results: </strong>BON supplementation resulted in 9% body weight increase (p = 0.027) and improved some body measurements (p<0.05). The calves in BON group showed a lower fecal consistency index (p<0.05) and required fewer medical treatments compared to CON and IOR (p = 0.103). Blood analysis revealed 27% reduction in globulin levels (p = 0.022), 10% increase in albumin levels (p = 0.023) and a 42% elevation in albumin/globulin ratio (p = 0.042) in BON compared to the CON groups. The activities of liver enzymes were higher in IOR than in the BON and CON group.</p><p><strong>Conclusion: </strong>Milk fortification with advanced chelated trace minerals significantly enhanced growth performance and health indicators in dairy calves, while inorganic sources showed no beneficial effects. These findings establish the superiority of chelated mineral supplementation for early-life dairy calf development;nevertheless, appropriate supplementation procedures require additional experiments.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1694-1707"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of DNA methylation changes following Cfp1 knockout in mouse spermatocytes.","authors":"Chanhyeok Park, Youngsok Choi, Seonho Yoo, Hyeonwoo La, Kwonho Hong","doi":"10.5713/ab.24.0807","DOIUrl":"10.5713/ab.24.0807","url":null,"abstract":"<p><strong>Objective: </strong>Spermatogenesis is a complex biological process that encompasses meiosis in spermatocytes and the dynamic epigenetic alterations that ensure the inheritance of genetic traits. CXXC finger protein 1 (CFP1, Cfp1, CXXC1, Cxxc1) is a critical component of the SET domain-containing 1A histone lysine methyltransferase complex that catalyzes histone H3K4 methylation and has a specific binding domain for unmethylated CpG DNA. However, our current understanding of CFP1's role in the genome-wide regulation of DNA and H3K4 methylation remains limited.</p><p><strong>Methods: </strong>We performed genome-wide methylation analysis using reduced-representation bisulfite sequencing on spermatocytes isolated from Cfp1 knockout and wild-type mice. Promoter methylation changes were integrated with publicly available microarray and ChIP-seq data to identify genes regulated by CFP1.</p><p><strong>Results: </strong>CFP1 depletion led to significant alterations in DNA methylation, particularly in promoter regions of genes associated with meiosis, transcription regulation, and chromatin remodeling. A total of 21 genes were identified as direct targets of CFP1, exhibiting reduced promoter methylation and CFP1 binding.</p><p><strong>Conclusion: </strong>Our study findings contribute to elucidating the regulatory mechanisms of CFP1 in spermatocytes, providing valuable insights into the reproductive process and advancing our understanding of the underlying causes of infertility.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1570-1579"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}