Animal Bioscience最新文献

{"title":"Key Events in the Process of Sex Determination and Differentiation in Early Chicken Embryos.","authors":"Xiaoqian Lv, Changhua Sun, Xin Liu, Guanzheng Liu, Wei Gong, Hongwu Qian, Zeyv Li, Jun Wu, Xilin Zhu, Jiuzhou Song, Yingjie Niu, Hongyan Sun, Wei Han, Guo Hong Chen, Kai Jin, Qisheng Zuo, Bichun Li","doi":"10.5713/ab.24.0679","DOIUrl":"https://doi.org/10.5713/ab.24.0679","url":null,"abstract":"<p><p>Clarifying the intricate mechanisms underlying sex determination and differentiation regulation in chickens holds significant potential for advancing chicken sex control technologies. This endeavor not only augments fundamental research in developmental biology but also bolsters the economic viability of the poultry industry. Our investigation delved into the sex determination and differen-tiation molecular underpinnings of these processes during the early stages of chicken embryonic development E0 d blastocysts, E3.5-E6.5 d genital ridges, and E18.5 d gonads, leveraging high-throughput sequencing technologies. Isolate ESC and CEF cells to verify roles of glycolysis, histone acetylation, and DNA methylation in sex determination. Treat E18.5 d gonads with gly-colytic activators (SB431542 and PD0325901, 2i), DNA demethylation activators (Vitamin C, VC), or inhibitors of histone acetylation modification (Valproic Acid, VPA) and analyze gender related gene expression, hormone levels, and gonad morphology. Key findings revealed that sex differences emerged as early as the blastocyst stage, intensifying with embryonic development and marked by a surge in sexually dimorphic gene expression. GO and KEGG analyses highlighted the pivotal roles of energy metabolism and epigenetic modification process during this critical period. 2i, VC, or VPA interventions targeting E18.5 embryo gonads, induced a remarkable transformation of ovarian tissue into a testis-like structure, characterized by cortical thinning, medulla densification, down-regulation of female-specific genes (Foxl2, Wnt4), upregulation of male-specific genes (Sox9, AMH), and increased testosterone secretion. This phenotypic and molecular shift underscores the ability of metabolic and epigenetic modulators to reprogram ovarian development towards a male-like pat-tern, preserving male sexual characteristics. Collectively, our findings underscore the fundamental importance of energy metabolism and epigenetic modifications in orchestrating sex determination and differentiation during early chicken embryonic development. This work lays a solid foundation for future endeavors aimed at unraveling the intricate regulatory networks governing these vital processes in chickens.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of milk fortification with an advanced chelated trace minerals supplement on growth, health, and nutrient digestibility in dairy calves.","authors":"Hossein Rajaei-Sharifabadi, Hadi Rahmani, Zahra Shokri, Saideh Fakharzadeh, Somayeh Kalanaky, Mohammad Hassan Nazaran, Ahmad Reza Seradj","doi":"10.5713/ab.24.0701","DOIUrl":"https://doi.org/10.5713/ab.24.0701","url":null,"abstract":"<p><strong>Objective: </strong>Recent advancements in Chelate Compound technology offer improved bioavailability of trace minerals, potentially enhancing growth performance and reducing disease incidence. Milk fortification provides an efficient way to supplement young calves before they transition to solid feed. This study evaluated the response of dairy calves to milk fortification with an advanced chelated trace minerals supplement containing Zn, Mn, Cu, Fe, Co, Cr, and Se, each selected for their specific roles in calf development, synthesized using Chelate Compounds technology.</p><p><strong>Methods: </strong>Forty-two Holstein dairy calves (21 male and 21 female) were randomly assigned to three experimental groups: control (CON, unfortified milk), chelated minerals (BON, milk fortified with chelated trace minerals), and inorganic minerals (IOR, milk fortified with inorganic trace minerals). Calves received their respective milk treatments daily from d 3 until weaning at 70 d of age. Growth performance and body measurements were measured throughout the experimental, while rectal temperature, fecal consistency, and frequency of medical treatment were recorded until d 21. Blood metabolites and nutrient digestibility were also determined.</p><p><strong>Results: </strong>BON showed significant positive effects on body weight and some body measurements, with trends towards improved average daily gain and feed efficiency. The calves in BON group showed a lower fecal consistency index and lower frequency of medical treatments compared to CON and IOR. Lower globulin levels as well as higher levels of albumin and albumin/globulin ratio were observed in BON compared to the other two groups. The activities of liver enzymes were higher in IOR than BON and CON group.</p><p><strong>Conclusion: </strong>These findings suggest that milk fortification with advanced chelated trace minerals have the potential to improve growth performance and health in dairy calves, while inorganic sources showed no benefits. Further research is needed to determine optimal supplementation levels and long-term effects on health and productivity of dairy calves.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leucyl-tRNA Synthetase Regulates Casein Synthesis in Dairy Cows via the mTORC1-LAT1 Pathway.","authors":"Yongding Ke, Ximeng Du, Binglan Chen, Xi Chen, Chengchuang Song, Xingtang Fang, Yanhong Wang, Chunlei Zhang","doi":"10.5713/ab.24.0711","DOIUrl":"https://doi.org/10.5713/ab.24.0711","url":null,"abstract":"<p><strong>Objective: </strong>Leucyl-tRNA synthetase (LARS) is an essential multifunctional enzyme in mammals, pivotal in maintaining cellular protein and amino acid equilibrium. It facilitates tRNA aminoacylation, initiating intracellular protein synthesis, and serves as an intracellular leucine sensor. This sensor function enables LARS to activate the mTORC1 pathway via Rag GTPase binding, playing a critical role in protein synthesis regulation. Despite its significance, the precise mechanisms of these functions are yet to be fully delineated. This study focuses on LARS, specifically its role in modulating milk protein synthesis.</p><p><strong>Methods: </strong>In this study,the bovine mammary epithelial cells were selected as the research subject, and stable LARS-OE and LARS-KD cell lines were constructed, which were verified by Cell Counting Kit-8, Click-iT EdU , Western blot, RT-qPCR, and immunoconfocal techniques.</p><p><strong>Results: </strong>Our findings show that LARS overexpression in bovine mammary epithelial cells (MAC-T) not only enhances cell proliferation but also mediates intracellular leucine levels, thereby increasing casein production through the mTORC1 pathway. LARS further boosts casein expression via the mTORC1-LAT1(mechanistic Target of Rapamycin Complex 1, L-type Amino Transporters 1) pathway. Remarkably, this interaction is supported by a positive feedback mechanism from LAT1, enhancing the activation of the mTORC1 pathway. Additionally, LARS overexpression leads to increased LAT1 expression, improved LAT1 stability, and its augmented localization at the membrane. Our research indicates that LARS's enhancement of LAT1 expression is contingent on its dual roles in translation and leucine sensing, whereas its impact on LAT1 localization is exclusively dependent on its leucine sensing function.</p><p><strong>Conclusion: </strong>s: In summary, by detecting intracellular leucine levels, LARS regulates LAT1 expression and membrane positioning through the mTORC1 pathway, ultimately influencing casein synthesis. These insights lay a theoretical groundwork for advancing milk protein production and offer novel strategies for improving dairy product quality.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between ambient temperature and body weight of Hanwoo (Korean native cattle).","authors":"Hyun Sang Kim, Jungeun Kim, Pilnam Seong, Won-Young Lee, Seongshin Lee, Jisoo Wi, Hye Ran Kim, Sung Dae Lee, Yookyung Lee","doi":"10.5713/ab.24.0489","DOIUrl":"https://doi.org/10.5713/ab.24.0489","url":null,"abstract":"<p><strong>Objective: </strong>Changes in ambient temperature negatively impact livestock productivity, with thermal stress causing physiological changes that affect beef quality and quantity. The calf stage is critical, as thermal stress during this period can have lasting effects on productivity. This study examined the impact of weather conditions on calf growth, carcass traits, and their interrelationships in Korean native Hanwoo steers.</p><p><strong>Methods: </strong>Data from 8,727 male Hanwoo calves were selected from 41,107 data points. Meteorological data were collected from 60 cities in South Korea between 2016 and 2019. The input data included calf growth, carcass traits, and weather conditions at birth for each calf.</p><p><strong>Results: </strong>Temperature, relative humidity (RH), and temperature-humidity index (THI) increased from winter to summer and decreased from summer to winter. Summer-born calves had significantly lower body birth weight (BBW) than those born in winter. The average daily gain (ADG) of the calves was highest in winter and lowest in spring and fall. A high carcass weight (CW) was observed in steers born in summer and winter. BBW and ADG were negatively correlated with ambient temperature, RH, and THI. Positive relationships were observed between BBW, ADG, and CW. Only CW was positively correlated with the meat quality grade of steers. Thus, temperature negatively affects the BBW of male Hanwoo calves, in turn affecting the daily gain, CW, and meat quality grade.</p><p><strong>Conclusion: </strong>This study demonstrates that temperature, relative humidity, and THI adversely affect the BBW of male Hanwoo calves, leading to reduced ADG and influencing CW and meat quality grade. Negative correlations were observed between BBW, ADG, and environmental factors, while CW showed positive correlations with BBW, ADG, and meat quality grade. These findings highlight the critical impact of climatic conditions on the growth performance and productivity of Hanwoo cattle.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection signature analysis using whole genome resequencing data reveals candidate genes for white plumage color in Korean native ducks.","authors":"Jaewon Kim, Jaegwon Kim, Eunjin Cho, Sunghyun Cho, Minjun Kim, Won-Hyong Chung, Jungwoo Choi, Hyo Jun Choo, Jun Heon Lee","doi":"10.5713/ab.24.0718","DOIUrl":"https://doi.org/10.5713/ab.24.0718","url":null,"abstract":"<p><strong>Objective: </strong>Domestication alters the phenotypes of wild animals to meet human demands and leaves characteristic patterns in their genomes. Various selection signature analysis methods have been developed to identify these characteristic patterns left in the genome. The Korean native duck (KND) is one of the domesticated species in Korea. KND is categorized into two populations based on plumage color; colored KND (KNDC) and white KND (KNDW). To enhance the competitiveness of native ducks, it is necessary to establish a KNDW line. In this study, we conducted selection signature analysis to identify candidate genes associated with white plumage color in KNDs.</p><p><strong>Methods: </strong>We generated whole genome resequencing data from 22 KNDCs, 22KNDWs, and 10 Pekin ducks (PKDs). To detect distinct genomic regions between KND populations with different plumage colors, we analyzed three types of selection signature analysis: the fixation index (Fst), nucleotide diversity(π), and cross-population extended haplotype heterozygosity (XP-EHH).</p><p><strong>Results: </strong>Population structure analysis showed that although KNDC and KNDW are distinct from PKD, they form a single group sharing a common ancestor. The results of Fst and π analyses revealed that compared to KNDC, there were strong selection signals in the MITF gene in KNDW, with a 6,641 bp insertion in the intron 2 region. This variant is a transposable element insertion that causes white plumage in PKD. In addition, XP-EHH analysis identified DCT, KIT, TYR, and ADCY9 as major candidate genes associated with pigmentation in the KND population.</p><p><strong>Conclusion: </strong>White plumage in KNDW is caused by a transposable element insertion in the MITF gene. This finding improves our understanding of plumage color in KND and supports the establishment of KNDW breeding programs.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles as an alternative treatment for bovine mastitis: state-of-the-art review.","authors":"Beatriz Elena Castro-Valenzuela, Moises Armides Franco Molina, Cristina Rodríguez Padilla","doi":"10.5713/ab.24.0590","DOIUrl":"https://doi.org/10.5713/ab.24.0590","url":null,"abstract":"<p><p>Nanotechnology is causing revolutionary changes in different disciplines such as food, pharmacology, medicine, and animal production. Through nanotechnology, it is possible to develop materials or devices with unique physical, chemical, and biological properties, different from those of their precursor materials, such as nanoparticles. These have gained special relevance in veterinary medicine, promising to be the future in treating bovine mastitis. Losses in the global dairy industry of up to USD 35 billion annually caused by this pathology have been determined. Despite implementing management practices and advances in the genetic selection of dairy cattle, the control of bovine mastitis remains inadequate. The most common therapy for your treatment is the use of antibiotics. However, the presence of residues of these drugs in milk compromises its quality. Furthermore, the emergence of bacterial resistance has caused the need to seek alternative strategies for treating infections in the mammary gland, opening a field of opportunities for the therapeutic use of nanoparticles. This review addresses key topics to elucidate the potential use of these nanoparticles as a treatment method for bovine mastitis.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of Biopolymer Infusion on Quality Traits of Pork Sausages during Storage.","authors":"Sang Keun Jin, Donggyun Yim","doi":"10.5713/ab.24.0743","DOIUrl":"https://doi.org/10.5713/ab.24.0743","url":null,"abstract":"<p><strong>Objective: </strong>We aimed to verify the impacts of cellulose/chitosan addition on the physicochemical properties of sausages.</p><p><strong>Methods: </strong>Sausages were prepared using 3% cellulose or chitosan and compared with those without biopolymers (control).</p><p><strong>Results: </strong>Sausages containing chitosan exhibited decreased pH values (p< 0.05), whereas those containing cellulose exhibited increased pH values. TBARS (2-thiobarbituric acid reactive substances) values did not differ between the control and biopolymer samples. The biopolymer samples had lower VBN contents than the control samples after 4 weeks of storage (p<0.05). The addition of chitosan significantly reduced the microbiological counts of sausages. Moreover, the infusion of chitosan led to lower lightness (L*) and whiteness (W) values but higher redness (a*), yellowness (b*), chroma (C*), and hue-angle (h°) values (p<0.05). The sausages containing chitosan exhibited significantly better textural parameters, except for springiness and gumminess, during storage.</p><p><strong>Conclusion: </strong>In conclusion, the addition of chitosan could reduce microbial growth and prolong the expiration date of sausages without compromising quality.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative effects of Dracocephalum moldavica L. and probiotic on performance and health parameters of broiler chickens challenged by dexamethasone-induced stress.","authors":"Seyed Heidar Hayatolgheibi, Mahdi Hedayati, Saeed Khalaji, Hossein Rajaei-Sharifabadi, Ahmad Reza Seradj","doi":"10.5713/ab.24.0698","DOIUrl":"https://doi.org/10.5713/ab.24.0698","url":null,"abstract":"<p><strong>Objective: </strong>Various environmental stressors impair poultry productivity by increasing oxidative stress. This study evaluated the performance and health status of broilers supplemented with either the medicinal plant Dracocephalum moldavica L. powder (DP) or a commercial probiotic (Lactofeed) under oxidative stress induced by dexamethasone.</p><p><strong>Methods: </strong>A total of 300 one-day-old chicks were enrolled in a completely randomized design with a factorial arrangement of six treatments, each consisting of five replicates of 10 chicks. The experimental treatments were: 1) basal diet without feed additives and no challenge, 2) basal diet with 0.01% Lactofeed, 3) basal diet with 0.4% DP, 4) basal diet and challenged with dexamethasone (2 mg/kg body weight), 5) basal diet with 0.01% Lactofeed and challenged with dexamethasone, and 6) basal diet with 0.4% DP and challenged with dexamethasone. Growth performance, carcass traits, and internal organ weights were evaluated. Gastrointestinal pH and ileum histology were assessed on days 28 and 42, while cecal microbial counts were determined on days 14, 28, and 42. Antibody titers against Newcastle disease and avian influenza viruses, as well as white blood cell counts were measured in serum samples collected on days 14, 28, and 42.</p><p><strong>Results: </strong>Dexamethasone challenge negatively impacted performance, select carcass traits, white blood cell count, and ileum villus height (p=0.039), while reducing cecal E. coli and coliform counts (p=0.001). It suppressed humoral immune response to Newcastle disease virus on day 28 but enhanced it at day 42. Lactofeed and DP additives significantly improved feed intake, body weight gain, white blood cell count, intestinal histology, and microbiota, largely independent of dexamethasone challenge.</p><p><strong>Conclusion: </strong>While dexamethasone challenge induced various physiological stresses in broilers, both Lactofeed and DP feed additives demonstrated potential in mitigating these effects, improving overall performance and gut health parameters regardless of stress conditions.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal dietary vitamin B1 content enhanced egg production, eggshell thickness, and serum antioxidant status in breeder geese.","authors":"Lin Dai, Baowei Wang, Qian Li, Mingai Zhang, Jing Zhang, Bin Yue, Min Kong, Binghan Wang, Wenlei Fan","doi":"10.5713/ab.24.0751","DOIUrl":"https://doi.org/10.5713/ab.24.0751","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the effects of vitamin B1 (VB1) supplementation on laying performance, egg quality, serum biochemical parameters, antioxidant status, and nutrient digestion in breeder geese.</p><p><strong>Methods: </strong>A total of 150 geese (30 males and 120 females, aged 34 weeks) were randomly assigned to 6 dietary treatment groups, each 5 replicates of 5 birds (1 male and 4 females). The geese were fed a basal diet supplemented with 0 (control), 1, 2, 3, 4, or 5 mg/kg of VB1 for 10 weeks.</p><p><strong>Results: </strong>VB1 supplementation had no significant effects on average feed intake, average egg weight, feed-to-egg ratio, egg shape index, eggshell strength, protein height, and Haugh unit (P > 0.05). However, it increased egg-laying rate, eggshell thickness, and yolk color (P < 0.05) in a quadratic manner, with the maximum values observed at 2 mg/kg VB1. Supplementing 2 mg/kg VB1 reduced serum aspartate transaminase activity (P < 0.05), but did not affect serum alanine transaminase activity, lipid and protein concentrations (P > 0.05). Serum glutathione peroxidase and total superoxide dismutase activities were enhanced by VB1 supplementation (P < 0.05), while total antioxidant capacity and malondialdehyde concentration remained unchanged (P > 0.05). Additionally, VB1 supplementation at 2 mg/kg increased crude ash digestibility, but did not affect the digestibility of ether extract, crude protein, calcium, and phosphorus.</p><p><strong>Conclusion: </strong>Dietary supplementing VB1 improved egg-laying performance, egg quality, antioxidant status, and mineral absorption in breeder geese. The optimal dietary VB1 concentration ranged from 3.83 to 4.81 mg/kg for improving egg production and quality, while higher concentrations of 4.99 to 6.51 mg/kg were needed to boost serum antioxidant status.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of low-purine diet supplemented with Sida acuta Burm. f. on growth performance, purine deposition, and biomolecules in slow-growing chickens.","authors":"Orapin Jantasaeng, Michel Jacques Duclos, Kanjana Thumanu, Supattra Okrathok, Sutisa Khempaka","doi":"10.5713/ab.24.0798","DOIUrl":"https://doi.org/10.5713/ab.24.0798","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the effects of a low-purine (Pu) diet supplemented with Sida acuta Burm. f. (SA) on growth performance, serum uric acid (SUA), and meat quality, including chemical composition, biomolecules, and purine deposition, in slow-growing Korat chickens (KRC).</p><p><strong>Methods: </strong>A total of 480 mixed-sex one-d-old KRC were randomly allocated into five groups with six replicates each (16 chicks/replicate) using a 1+2×2 augmented factorial experiment in a completely randomized design. Five experimental diets were as follows: a control (basal diet), two diets with 30 and 45% lower purine levels than the control (-30 and -45% Pu), each supplemented with two levels of SA (0.3 and 0.6% SA), respectively. After sex determination, all subsequent analyses were conducted exclusively on female birds.</p><p><strong>Results: </strong>KRC fed a -30% Pu diet supplemented with 0.6% SA showed reduced hypoxanthine and total purine deposition in major breast muscle (Pectoralis major) without negative effects on growth performance, SUA levels, meat quality, chemical composition, biomolecules, and protein secondary structures comparable to the control group. Birds fed a -45% Pu diet, supplemented with either 0.3 or 0.6% SA, exhibited impaired growth performance and without significant changes in major breast muscle hypoxanthine or total purine content. Correlation analysis revealed that the -30% Pu diet supplemented with 0.6% SA was positively correlated with reduced hypoxanthine and total purine content, while showing negative correlations with lipid content and ß-turn structure.</p><p><strong>Conclusion: </strong>A 30% reduction in dietary purines combined with 0.6% SA supplementation effectively decreased purine deposition in chicken meat, contributing to the production of healthier chicken meat product. Further studies are needed to investigate the molecular mechanisms and metabolic pathways, which will enable breeders to advance the development of low-purine chicken meat production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}