Animal Bioscience最新文献

{"title":"Association between Weaning Stress and Rumen Microbiota in Goat Kids: Evidence from Granger Causality and Randomized Controlled Trial Validation.","authors":"Ziwei Peng, Hanjie Xiao, Weiwei Yang, Tong Wang, Haojiong Xie, Hui Yan, Shoukun Ji","doi":"10.5713/ab.25.0092","DOIUrl":"https://doi.org/10.5713/ab.25.0092","url":null,"abstract":"<p><strong>Objective: </strong>Weaning stress is frequently associated with disrupted gastrointestinal microbiota and decreased growth performance, the association between them remains unclear.</p><p><strong>Methods: </strong>In this study, we employed Granger causality inference to compare the dynamic changes in gastrointestinal microbiota between stressed and non-stressed goat kids, followed by validation through a randomized controlled trial involving rumen microbiota transplantation.</p><p><strong>Results: </strong>Our findings indicated that the alpha diversity and microbial maturity of the rumen microbiota in stressed kids were significantly lower than those in non-stressed kids during the weaning period (p<0.05). Furthermore, the rumen microbiota at pre-weaning could accurately predict the body weight changes during weaning stress period (R2=0.99, RMSPE=0.19). We demonstrated that the cultured rumen microbiota retained most high-abundance rumen bacteria (89.22%), and its transplantation effectively altered the rumen microbiota (ANOSIM, p<0.05), enhanced microbial maturity (p<0.05), and improved growth performance (p<0.05) during weaning, without affecting the gut microbiota (p>0.05). Additionally, transplantation of the cultured rumen microbiota reduced intestinal permeability and inflammation while increasing antioxidant levels in weaned kids(p<0.05).</p><p><strong>Conclusion: </strong>These findings provide evidence for the association between rumen microbiota and weaning stress, demonstrating that manipulating rumen microbiota is an effective strategy for alleviating weaning stress in goat kids.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Light Duration and Variation on the Growth and Reproductive Performance in Breeder Geese.","authors":"Min Jung Lin, Shen Chang Chang, Li Jen Lin, Jhih Siang Chang, Shao Yu Peng, Tzu-Tai Lee","doi":"10.5713/ab.25.0349","DOIUrl":"https://doi.org/10.5713/ab.25.0349","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to investigate the effects of time and light variation on the growth and reproductive performances of breeder geese.</p><p><strong>Methods: </strong>A total of 32 ganders and 96 dames in their first laying season, with an average age of 10 months, were assigned to eight rooms, each containing four ganders and twelve dams. A split-plot design was used, incorporating two prelay photoperiod treatments: a 7-hour light period (P7H), in which the photoperiod was maintained at seven hours for the first six weeks, and a gradually decreasing light (GDL) group, in which the light duration was initially set at 12 hours and then reduced by one hour per week during the prelay period. Two variable photoperiod schedules were applied as sub-treatments and randomized across the rooms. In one treatment, the egg production rate in breeder geese decreased to an average below 30%, after which the photoperiod increased by 15 minutes each week (change time, CHP). A fixed photoperiod of nine hours was maintained (fixed time, FIXP) until the end of the egg-laying period.</p><p><strong>Results: </strong>Geese in the GDL light group had a longer laying duration than P7H light group (243.75 vs 191.75 days; p<0.01). Number of eggs per goose in the GDL light group showed a trend toward higher values than P7H light group. However, the fertility and hatchability in GDL light group were significantly lower than the P7H light group at all periods, respectively.</p><p><strong>Conclusion: </strong>Geese exposed to a 12-hour light (12L:12D) regimen during the pre-laying period exhibited a longer laying duration and higher egg production per bird. However, maintaining a fixed lighting schedule of 9 hours of light and 15 hours of darkness (9L:15D) after the peak laying period is recommended to optimize production profitability by supporting better fertility and hatchability.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions between extracellular vesicles and hormones.","authors":"Yiwen Bi, Yang Yang, Linsheng Gan, Wenhui Huang, Xin Feng, Huihua Zhang, Jie Liu, Limin Wei, Qien Qi","doi":"10.5713/ab.25.0249","DOIUrl":"https://doi.org/10.5713/ab.25.0249","url":null,"abstract":"<p><p>This review explores the interaction between extracellular vesicles (EVs) and hormones, highlighting their importance in physiological and pathological conditions. As key messengers of intercellular communication, extracellular vesicles are rich in a variety of bioactive molecules and can play an important role in the in vivo environment. Hormones, as the core mediators of regulating the functions of organisms, affect the production and release of extracellular vesicles through a variety of signaling pathways, and then regulate the function of target cells. Conversely, extracellular vesicles can also carry biologically active molecules that significantly affect hormone synthesis and secretion. In this review, the effects of different hormones (such as estrogen, testosterone, follicle-stimulating hormone, etc.) on extracellular vesicles and the regulatory effects of extracellular vesicles on hormone secretion are discussed in detail, which reveals the potential clinical application value of this field, including the possibility of being a biomarker and a new direction of personalized medicine. Future studies are needed to further understand the molecular mechanism of this interaction to promote the development of new diagnostic tools and therapeutic strategies.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semirefined glycerin as an alternative energy source on intake, digestibility and milk production of lactating cows.","authors":"Murilo de Almeida Meneses, Robério Rodrigues Silva, Dicastro Dias de Souza, Eli Santana de Oliveira Rodrigues, Wendel Bispo Santos E Santos, Lara Maria Santos Brant, José Esler Freitas Júnior, Fabiano Ferreira da Silva","doi":"10.5713/ab.25.0319","DOIUrl":"https://doi.org/10.5713/ab.25.0319","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the effect of dietary glycerin levels on feed intake, nutrient digestibility, milk yield and composition, nitrogen balance, microbial production, and eating behavior in confined lactating cows.</p><p><strong>Methods: </strong>Eight crossbred cows (Holstein × Zebu) were used, with an average of 90 ± 10 days in milk, milk production of 21.3 ± 0.47 kg/day, and body weight of 570 ± 51 kg. The cows were assigned to two 4×4 Latin squares with the following treatments: Control (G0), no glycerin inclusion; G6, 6% glycerin inclusion in the total dietary dry matter (DM); G12, 12% glycerin inclusion; and G18, 18% glycerin inclusion.</p><p><strong>Results: </strong>Glycerin inclusion linearly decreased DM intake (p<0.001), crude protein (CP) intake (p=0.004), and neutral detergent fiber (NDF) intake (p<0.001). On the other hand, the digestibility coefficients of DM (p=0.001), CP (p=0.007), non-fiber carbohydrates (NFC) (p<0.001), and total digestible nutrients (TDN) (p=0.013) increased linearly. Milk yield (P=0.046) and 3.5% fat-corrected milk (FCM) (p=0.013) decreased linearly.</p><p><strong>Conclusion: </strong>Despite increasing the digestibility of nutrients, the inclusion of glycerin in diets for feedlot dairy cows resulted in a significant reduction in dry matter intake, which compromised total energy intake and, consequently, milk production. Thus, although it is a promising co-product due to its availability and energy value, semi-refined glycerin, in the proportions tested, was not viable as an energy substitute in diets of lactating cows, requiring caution not to compromise productive performance.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Replacement of soya DOC with rice DDGS and its effect on performance, nutrient metabolizability, carcass traits and gut health of broiler chicken.","authors":"Atul Parashuram Dhok, Roshan Lavakumar Werulakar, Sudhir Bhimrao Kawitkar, Nitin Vasantrao Kurkure, Pratiksh Kaluram Bacche, Mahesh Ravindra Jawale, Shital Vitthalrao Chopde, Roupesh G Roupessss, Darshana B Bhaisare, Madhuri S Hedaoo, Shilpa V Shinde, Shweta Ramesh Lende","doi":"10.5713/ab.23.0533","DOIUrl":"https://doi.org/10.5713/ab.23.0533","url":null,"abstract":"<p><strong>Objective: </strong>- The study was conducted to utilize rice DDGS as a protein source in broiler chicken.</p><p><strong>Methods: </strong>- Three hundred day old straight run commercial broiler chicks were divided into five groups of four replications each and fed as CG control receiving standard basal diet; 15-RDDGS with 15 percent rice DDGS; 15-RDDGSE with 15 percent rice DDGS supplemented with protease enzyme @ 300g/ton of feed; 20-RDDGS with 20 percent rice DDGS and 20-RDDGSE with 20 percent rice DDGS supplemented with protease enzyme @ 300g/ton of feed.</p><p><strong>Results: </strong>- The final body weights were significantly (p<0.01) higher and FCR was significantly better for CG and comparable with 15-RDDGS and 15-RDDGSE. The CP metabolizability, was significantly (p<0.05) higher for CG than 15 and 20 percent rice DDGS groups, however the CP metabolizability for protease supplemented 15 and 20 percent rice DDGS group was comparable with control diet. The total viable count (TVC) and E-coli count revealed non-significant differences. There was no negative impact on histological changes in intestine due to inclusion of rice DDGS. There was no negative impact on histological changes such as crypt depth, villus width and villus height in intestine due to interaction between DDGS and enzyme. The carcass traits revealed non-significant differences for dressing percentage, overall giblet weight and abdominal fat.</p><p><strong>Conclusion: </strong>- It was concluded that rice DDGS can be incorporated upto 20 levels in broiler diet without any adverse effect, however inclusion of 15 percent rice DDGS supplemented with protease enzyme proved to be more economical in broilers.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of injured muscle identifies new candidate genes for satellite cell growth and myofiber formation during early muscle regeneration.","authors":"Zhuning Yuan, Xian Tong, Xianyao Luo, Liping Pan, Hoi-Ka Wu, Rong Xu, Ziyun Liang, Xunhe Huang, Delin Mo","doi":"10.5713/ab.24.0859","DOIUrl":"https://doi.org/10.5713/ab.24.0859","url":null,"abstract":"<p><strong>Objective: </strong>: The self-repair capacity of skeletal muscle arouses interest in studying satellite cell activity and myofiber formation. The major molecular networks of satellite cell activity have been extensively studied. However, the mechanism by which micro-environmental factors regulate satellite cell activity for early muscle regeneration still remains poorly understood.</p><p><strong>Methods: </strong>: Hematoxylin and eosin (HE) staining and immunofluorescence for embryonic myosin heavy chain (eMyHC) were performed on control and injured muscle samples at 12-, 24-, 36-, 48-, 60-, 72-, and 84-hour post-injury. Additionally, eMyHC immunofluorescence was conducted on muscle samples collected 96 hours post-injury from three mice. RNA sequencing (RNA-seq) and quantitative PCR (qPCR) were performed on samples from 24 mice, including controls and samples at 12-, 24-, and 84-hour post-injury.</p><p><strong>Results: </strong>: In this research, 516 immune-related and 177 hormone response-related genes were up-regulated significantly. Further, statistical analysis indicated that the number of differentially expressed genes (DEGs) between up- and down-regulated immune system-related DEGs was similar with that of hormone response-related DEGs. Notably, p53 signaling pathway was significantly enriched throughout early muscle regeneration. Based on patterns of crucial myogenic genes expression, 326 and 320 candidate genes related to satellite cell growth and myofiber formation were obtained, respectively. Furthermore, through interaction network analysis, 41 immune factors, including S100a9，Csf3r，Cxcl3，Ppbp，Ccl3，Il1rn were found, which may regulate satellite cell activation, migration and proliferation. Likewise, 16 cell adhesion factors (Col1a2, Cdh2, Thbs2, etc.) may be involved in myofiber formation.</p><p><strong>Conclusion: </strong>: This study leveraged transcriptomic analysis to uncover key candidate genes and biological processes involved in early muscle regeneration. These findings enhance our understanding of the molecular mechanisms underlying muscle repair and offer insights for future therapeutic strategies.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of active and heat-killed Clostridium butyricum on in vitro gas production, ruminal fermentation, and microbiota varying with pH levels.","authors":"Xinlong Zhang, Zhiyue Zhang, Hongxu Zhu, Guanghui Hu, Hangshu Xin, Jincheng Liu, Xu Lin, Xiaolai Xie, Peixin Jiao","doi":"10.5713/ab.24.0913","DOIUrl":"https://doi.org/10.5713/ab.24.0913","url":null,"abstract":"<p><strong>Objective: </strong>: The objective of this study was to evaluate the effects of inactive (heat-killed) Clostridium butyricum (ICB) on gas production kinetics, fermentation parameters, and microbiota with varying media pH levels in batch culture.</p><p><strong>Methods: </strong>: The in vitro experiment was designed as a completely randomized factorial arrangement, with 2 media pH levels (5.8 and 6.5) × 2 CB products (active and inactive) × 4 dosages. Two lactating dairy cows with ruminal fistulas, fed a diet comprising 40% forage and 60% concentrate, served as donors for rumen inoculum. Following 24 h of incubation, the gas production, dry matter disappearance (DMD), volatile fatty acid (VFA), ammonia nitrogen (NH3-N) and microbial profile were analyzed to determine the effect of treatment on fermentation.</p><p><strong>Results: </strong>: The gas volume (GV), DMD, total VFA concentration, NH3-N concentration, acetate proportion and microbial alpha diversity were inhibited when the media pH decreased from 6.5 to 5.8. Increasing the supplemental doses of ICB linearly increased the GV, DMD (trend) and butyrate proportion at media pH 6.5. Moreover, the increasing supplemental dose of active Clostridium butyricum (ACB) linearly increased GV, butyrate proportion and NH3-N concentration (trend) regardless of media pH, and linearly increased DMD, total VFA concentration and A:P ratio at media pH 6.5. Supplementing ICB decreased the relative abundance of Actinobacteria and Butyrivibrio in the fermentation fluid.</p><p><strong>Conclusion: </strong>s: Increasing media pH would favor rumen fermentation and alter bacterial community. Although both ACB and ICB have the potential to stimulate rumen fermentation in a dose-dependent manner, their effects vary with different media pH levels. Moreover, both ACB and ICB rarely caused changes to the rumen bacterial community.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of Germ Cell-like Cells from Dazl-eGFP Knock-In Chicken Somatic Cells via Transgenic Expression of Pluripotency and Germ Cell-Specific Transcription Factors.","authors":"Bo Ram Lee, Hyeon Yang, Sun Keun Jung, Sung June Byun, Tae Sub Park","doi":"10.5713/ab.25.0233","DOIUrl":"https://doi.org/10.5713/ab.25.0233","url":null,"abstract":"<p><strong>Objective: </strong>: Germ cell identity is orchestrated by the coordinated action of multiple key transcription factors during embryonic development, involving the induction and regulation of germline-specific gene expression. In chickens, the expression of DEAD-box helicase 4 (DDX4) and Deleted in Azoospermia-like (DAZL) genes plays a pivotal role in germplasm formation and the specification of germ cell lineage from a totipotent genome. This study aimed to investigate the regulatory mechanisms underlying germ cell fate determination.</p><p><strong>Methods: </strong>: We performed large-scale gene expression profiling to screen and select critical transcription factors. Through this analysis, we identified differentially expressed genes (DEGs) in chicken primordial germ cells (PGCs), including 1,020 transcription factors. Additionally, we generated a chicken DF1 cell line with an enhanced green fluorescent protein (eGFP) reporter precisely knocked into the transcriptional start site of the DAZL gene using the CRISPR-Cas9 system, enabling real-time monitoring of DAZL expression during reprogramming.</p><p><strong>Results: </strong>: Through analysis of transcription factor binding sites within ~10 kb upstream regions of DDX4 and DAZL, we selected 10 candidate transcription factors for germ cell induction. Subsequently, the 10 transcription factors identified as regulators of germ cell identity were transduced into the DAZL-knock-in eGFP DF1 cells. This approach led to the successful induction of eGFP-expressing cells in vitro, driven by the endogenous DAZL promoter. We further characterized these cells to confirm their germ cell-specific properties.</p><p><strong>Conclusion: </strong>: Our findings offer new insights into the transcriptional regulation of chicken germ cells by identifying key factors responsible for the activation of DAZL expression. These results present valuable opportunities for advancing germ cell induction from somatic cells, with potential applications of in vitro models for studying germ cell-specific gene regulatory pathways in avian species.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural and crystal bundles disaggregated palygorskite in young broilers: A comparison study.","authors":"Zichao Tan, Yueping Chen, Chao Wen, Aiqin Wang, Yanmin Zhou","doi":"10.5713/ab.25.0150","DOIUrl":"https://doi.org/10.5713/ab.25.0150","url":null,"abstract":"<p><strong>Objective: </strong>: This study aimed to compare the effects of natural palygorskite (Nat-Pal) and crystal bundles disaggregated palygorskite (Dis-Pal) supplementation on the growth performance, intestinal health, and digestive function of young broilers.</p><p><strong>Methods: </strong>: A total of 400 male Arbor Acres broilers (one-day-old) were randomly assigned to five experimental groups with eight replicates over a 14-day experimental period. Chicks were fed a basal diet (Control group), the basal diet supplemented with 10 g/kg Nat-Pal or 2.5, 5, and 10 g/kg Dis-Pal, respectively.</p><p><strong>Results: </strong>: Dis-Pal linearly increased average body weight and average daily gain, and decreased feed to gain ratio of broilers from days 1 to 14, with the 5-10 g/kg Dis-Pal showing the most pronounced effects (P < 0.05). An addition of Dis-Pal linearly increased glucose level, and decreased triglyceride level in serum (P < 0.05). Compared to the control group, 5 g/kg Dis-Pal increased the digestibility of crude protein and dry matter, enhanced trypsin, lipase, and amylase activities in jejunal digesta, and elevated the ratio between villus height and crypt depth in jejunum and ileum (P < 0.05). Dis-Pal linearly increased ileal mucosal glutathione and total antioxidant capacity levels, as well as total superoxide dismutase and catalase activities, with 10 g/kg Dis-Pal addition showing the best effects (P < 0.05). The 2.5-10 g/kg Dis-Pal decreased the interferon-γ and tumor necrosis factor-α levels in ileal mucosa compared to the control group (P < 0.05). Moreover, Dis-Pal supplementation linearly increased interleukin-10, secretory immunoglobulin A, and immunoglobulin M levels, and decreased the interferon-γ level, quadratically increased total superoxide dismutase and catalase activities in jejunal mucosa (P < 0.05).</p><p><strong>Conclusion: </strong>: Dietary Dis-Pal supplementation could improve growth performance, intestinal health, and enhance the nutrients digestibility of young broilers. Supplementation with Dis-Pal was more advantageous for broilers than its Nat-Pal, and its optimal dosage was 5 g/kg.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome and microbiota analysis reveal differences in the cecum of weaning pigs in response to different dietary crude protein levels.","authors":"Yu-Hsiang Yu, Sheng-Bing Chen, Han-Tsung Wang, Chuan-Shun Lin, Andrzej Dybus, Beata Hukowska-Szematowicz, Yi-Hung Li","doi":"10.5713/ab.25.0135","DOIUrl":"https://doi.org/10.5713/ab.25.0135","url":null,"abstract":"<p><strong>Objective: </strong>: This study explored the effects of different dietary crude protein (CP) levels on the cecal transcriptome and microbial composition of weaning pigs.</p><p><strong>Methods: </strong>: A total of 96 weaning pigs were randomly assigned to 3 groups named the H, M, and L groups. The H, M, and L groups received 20% and 18%, 18% and 16%, and 16% and 14% CP in the early (1-14 days) and late phases (15-28 days), respectively.</p><p><strong>Results: </strong>: The final body weight and average daily gain were lower in the L group than in the other groups (p = 0.008). The feed conversion ratio was lower in the H and M groups than in the L group (p = 0.01). Cecal transcriptome analysis revealed that heatmap and principal component analysis of differentially expressed genes indicated the presence of distinct clusters between the groups. Genes involved in cell proliferation and differentiation and inflammation was downregulated in the M and L groups, compared with corresponding genes in the H group (p < 0.001). Pathway enrichment analysis suggested that genes related to IL-17 signaling pathway was downregulated in the M and L groups (p < 0.05). Beta diversity analysis and heatmap for microbial composition and function indicated the presence of distinct clusters between the groups. Carbohydrate-fermenting bacteria, such as Megasphaera elsdenii DSM 20460 and Blautia luti DSM 14534, were higher in the M and L groups compared with the H group (p ≤ 0.05). The abundance of Lactobacillus amylovorus DSM 20531 was higher in the M group than in the other groups (p ≤ 0.05). The abundance of L. amylovorus DSM 20531 was positively correlated with growth performance. Integrated multi-omics analysis suggested significant similarities between cecal transcriptome and microbiota (p < 0.01).</p><p><strong>Conclusion: </strong>: Reducing the level of CP modulate cell growth and alleviate inflammation in the cecum. A.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}