Animal Bioscience最新文献

{"title":"Effect of processing techniques on energy content and amino acid digestibility in corn germ meal fed to growing pigs.","authors":"Jinbiao Zhao, Zhaoyu Liu, Xiaoming Song, Meiyu Yang, Zhongchao Li, Ling Liu","doi":"10.5713/ab.24.0581","DOIUrl":"10.5713/ab.24.0581","url":null,"abstract":"<p><strong>Objective: </strong>The study was conducted to determine energy contents, apparent total tract digestibility (ATTD) of nutrients, the apparent (AID) and standardized (SID) ileal digestibility of amino acids (AA) in corn germ meals (CGM) produced by processing technologies of wet milling (CGM-CV1 and CGM-CV2), heating-dried CGM with steep liquor (CGMSL-DH) and dried using indirect heat (CGMSL-IH), corn germ expellers (CGE) and dry-grind processing method (CGM-DG).</p><p><strong>Methods: </strong>In Exp. 1, forty-two crossbred male barrows with an initial body weight (BW) of 51.2±4.5 kg were assigned to 1 of 7 diets in a randomized complete block design. In Exp. 2, seven cannulating barrows with an initial BW of 35.3±1.3 kg were assigned to 7 diets in each period according to a Latin square design. Each diet included 6 replicates.</p><p><strong>Results: </strong>The ATTD of acid detergent fiber and gross energy (GE), and SID of most AA, except for tryptophan (Trp) and cysteine (Cys), in CGMSL-IH was greater (p<0.05) than those in CGM-CV2. The ATTD of organic matter (OM), crude protein and GE, and SID of most AA, except for arginine (Arg), histidine (His), lysine (Lys) and tyrosine (Tyr), in CGMSL-DH was greater (p<0.05) than those in CGM-CV1 for pigs. The ATTD of GE and acid-hydrolyzed ether extract, contents of digestible and metabolizable energy (ME), and SID of AA, except for Arg, His, Lys, Phenylalanine, Trp, Cys, glutamic acid, glycine and Tyr, in CGE were greater (p<0.05) than those in CGM-CV1. The ATTD of OM and GE, and SID of all AA in CGM-DG were greater (p<0.05) than those in CGM-CV1. Compared with the CGM-CV1, CGM-CV2 showed a greater (p<0.05) ATTD of GE and SID of some AA for growing pigs.</p><p><strong>Conclusion: </strong>Different processing technologies lead to large variations in energy contents and AA digestibility of CGM for growing pigs. The CGE contains more available energy compared with CBM-CV1, and CGMSL-DH, CGMSL-IH and CGM-DG provides more ileal digestibility of AA for growing pigs compared with the CGM-CV1 and CGM-CV2.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1003-1014"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome resequencing reveals genetic differentiation and selection signatures among wild, local and commercial duck populations.","authors":"Zhirong Huang, Liyun Zhang, Maojun Luo, Xumeng Zhang, Yunmao Huang, Yunbo Tian, Zhongping Wu, Xiujin Li","doi":"10.5713/ab.24.0643","DOIUrl":"https://doi.org/10.5713/ab.24.0643","url":null,"abstract":"<p><strong>Objective: </strong>The purpose of this study was to systematically analyze the population genetic structure and genetic diversity among wild, local and commercial populations using whole-genome sequencing data from 416 individuals of 22 duck breeds in China and to further explore genetic pathways and candidate genes associated with importantly economic traits.</p><p><strong>Methods: </strong>We performed principal component analysis, an unrooted neighbor-joining phylogenetic tree and ADMIXTURE to analyze the population structure. We compared the genetic diversity among wild, local and commercial populations using the effective population size, inbreeding coefficient, expected heterozygosity, observed heterozygosity, nucleotide diversity and regions of homozygosity. To detect selection signatures, we calculated the locus-specific branch length for local and commercial populations and calculated genetic differentiation coefficient and genetic diversity between egg and dual-purpose breeds.</p><p><strong>Results: </strong>Wild, local and commercial duck populations formed three distinct genetic groups. The commercial population presented the lowest genetic diversity, the highest levels of inbreeding and the smallest effective population size. ADMIXTURE analysis also demonstrated that ducks were clearly divided into these three populations at K = 3. Selection signals in the commercial population were associated with growth and muscle development pathways, such as the mTOR signaling pathway and ErbB signaling pathway, and two key genomic regions (Chr1: 70.25 to 74.00 Mb and Chr2: 97.10 to 99.76 Mb) containing important genes, such as LRP6, BORCS5, and EDN1, were identified. In contrast, selection signals in the local population were associated with immune-related pathways involving NCAM2 and MPHOSPH6. Furthermore, PTGS2 and PLA2G4A genes were positively selected in egg breeds, whereas KCNK16, KCNK5, and KCNK17 genes were in dual-purpose breeds.</p><p><strong>Conclusion: </strong>Because of artificial selection, wild, local and commercial populations presented obvious genetic differences. The selection signal analysis revealed that LRP6, BORCS5, and EDN1 are important for growth and muscle development; NCAM2 and MPHOSPH6 are for immune traits; and PTGS2 and PLA2G4A are for egg-related traits.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":"38 5","pages":"898-909"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome and targeted metabolome analysis of lipid profiles, nutrients compositions and volatile compounds in longissimus dorsi of different pig breeds.","authors":"Zhen Luo, Ting Lai, Yijia Fan, Chengbin Yu, Wei Li, Meng Li, Shenghui Lei, Jing Zhang, Weina Xu, Zhe Wang, Jianxiong Xu","doi":"10.5713/ab.24.0564","DOIUrl":"10.5713/ab.24.0564","url":null,"abstract":"<p><strong>Objective: </strong>Improving meat quality is important for commercial production and breeding. The molecular mechanism of intramuscular fat (IMF) deposition and meat characteristics require further study.</p><p><strong>Methods: </strong>This study aimed to study the mechanism of IMF deposition and meat characteristics including redox potential, nutrients compositions and volatile compounds in longissimus dorsi (LD) by comparing with different pig breeds including Shanghai white (SW), Duroc×(Landrace Yorkshire) (DLY) and Laiwu (LW) pigs.</p><p><strong>Results: </strong>Results showed that the contents of IMF, triglyceride (TG), total cholesterol (TC), and redox potential parameters were lower, while the content of malondialdehyde (MDA) and activity of lactate dehydrogenase (LDH) were higher in LD of SW pigs compared with LW pigs (p<0.05). No differences were observed about these parameters between SW and DLY pigs. Also, the contents of medium-long chain fatty acids and γ-aminobutyric acid (GABA) were higher, while Asp was lower in LD of SW pigs compared with LW pigs (p<0.05). Volatile compounds results showed that 6 ketones, 4 alkenes, 11 alkanes, 2 aldehydes, 1 alcohol were increased, and cholesterol was decreased in SW pigs compared with LW pigs. Transcriptome results showed that differential expressed genes involved in lipid synthesis, metabolism and transport in LD between SW and LW pigs, which were further verified by quantitative polymerase chain reaction. Spearman correlation showed that HSL and Nedd4 were positively related to contents of TG and IMF, while negatively related to volatile compounds and fatty acids (p<0.05). Plin3 and Mgll were negatively related to contents of TG, IMF and cholesterol, while positively related to MDA, LDH, and volatile compounds (p<0.05). PPARA was negatively related to contents of TC and IMF, and activity of superoxide dismutase, while positively related to volatile compounds (p<0.05).</p><p><strong>Conclusion: </strong>Our study provided new insights into potential mechanisms of IMF deposition, nutrients composition and volatile compounds of muscular tissues of different pig breeds.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1053-1066"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The expression pattern of butyric acid transporter in the large intestine with growth and development of suckling lambs.","authors":"Jian Ma, Tao Li, Lu Lin, Chunmei Du, Chen Wei, Fuquan Yin, Xuemei Sun, Gang Lyu, Shangquan Gan","doi":"10.5713/ab.24.0490","DOIUrl":"10.5713/ab.24.0490","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this research was to explore the changes of morphological parameters, short chain fatty acid contents and butyric acid transporter expression levels in the large intestine with growth and development of sucking lambs.</p><p><strong>Methods: </strong>A total of 48 newborn male Hu sheep lambs (body weight = 2.94±0.22 kg) were selected in this experiment. At 0, 7, 14, 28 and 42 days of ages, 6 lambs were slaughtered to collect cecal and colonic samples to analyze morphological parameters, short chain fatty acid contents and butyric acid transporter mRNA expression.</p><p><strong>Results: </strong>The organ index of the cecum in d 0, 28 and 42 groups was higher (p<0.05) than that in d 7 and 14 groups. Compared with other age groups, the organ index of the colon was significantly increased (p<0.05) in d 42 group. After 7 days of age, the contents of acetic, propionic and butyric acids in the cecum and colon were significantly increased (p<0.05) as age increased. A similar trend of mRNA expressions of butyric acid transporters, including monocarboxylate transporter, sodium-coupled monocarboxylate transporter, sodium-hydrogen exchanger, down regulated in adenoma, putative anion transporter-1 and anion exchanger-2, was found. In the cecum, the Escherichia coli count in d 14 group was higher (p<0.05) than that in other age groups, whereas an opposite tendency was found in Lactobacillus count between d 14 and other groups. Additionally, in the cecum and colon, the relative expression of claudin-1 in d 14 group was lower (p<0.05) than in d 42 group.</p><p><strong>Conclusion: </strong>Overall, the current results indicate that the expression levels of butyric acid transporters in the cecum and colon of lambs are significantly influenced by days of age.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"968-980"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Key events in the process of sex determination and differentiation in early chicken embryos.","authors":"Xiaoqian Lv, Changhua Sun, Xin Liu, Guanzheng Liu, Wei Gong, Hongwu Qian, Zeyu Li, Jun Wu, Xilin Zhu, Jiuzhou Song, Yingjie Niu, Hongyan Sun, Wei Han, Guo Hong Chen, Kai Jin, Bichun Li, Qisheng Zuo","doi":"10.5713/ab.24.0679","DOIUrl":"10.5713/ab.24.0679","url":null,"abstract":"<p><strong>Objective: </strong>Current understanding of sex determination and differentiation mechanisms during early chicken embryonic development remains incomplete. To address this, we applied RNA sequencing to identify male-female expression differences at critical developmental stages (E0 blastocysts, E3.5-E6.5 genital ridges, E18.5 gonads), focusing on glycolysis, histone acetylation, and DNA methylation. This approach aims to unravel key regulatory mechanisms and advance developmental biology insights.</p><p><strong>Methods: </strong>We analyzed molecular mechanisms of chicken sex determination at key stages (E0 blastocysts, E3.5-E6.5 genital ridges, E18.5 gonads) using RNA sequencing. Glycolysis, histone acetylation, and DNA methylation levels were assessed in embryonic stem cells and chicken embryonic fibroblasts. E18.5 gonads were treated with glycolytic activators (SB431542 and PD0325901 [2i]), a DNA demethylation activator (Vitamin C [Vc]), or an inhibitors of histone acetylation modification (valproic acid [VPA]). Sex-related gene expression, hormone levels, and gonad morphology were evaluated to determine treatment effects.</p><p><strong>Results: </strong>Key findings revealed that sex differences emerged as early as the blastocyst stage, intensified with embryonic development and were marked by a surge in sexually dimorphic gene expression. Gene Ontology and Kyoto encyclopedia of genes and genomes analyses highlighted the pivotal roles of energy metabolism and epigenetic modification process during this critical period. 2i, VC, or VPA interventions targeting E18.5 embryo gonads, induced a remarkable transformation of ovarian tissue into a testis-like structure, characterized by cortical thinning, medulla densification, downregulation of female-specific genes (FOXL2, WNT4), upregulation of male-specific genes (SOX9, AMH), and increased testosterone secretion. This phenotypic and molecular shift underscores the ability of metabolic and epigenetic modulators to reprogram ovarian development towards a male-like pattern, preserving male sexual characteristics.</p><p><strong>Conclusion: </strong>Our study establishes energy metabolism and epigenetic regulation as central drivers of avian sex determination. These findings advance understanding of vertebrate developmental biology and provide a framework for dissecting regulatory networks in avian sexual development.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1081-1104"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-oil corn distillers dried grains with solubles can be fed to pigs up to 16.5% without compromising growth and pork quality.","authors":"Woon Sen Lee, Hyunwoong Jo, In Ho Kim, Beob Gyun Kim","doi":"10.5713/ab.24.0629","DOIUrl":"10.5713/ab.24.0629","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to determine the maximum inclusion rate of low-oil corn distillers dried grains with solubles (DDGS) in swine diets with a focus on growth performance and pork quality.</p><p><strong>Methods: </strong>A total of 160 crossbred pigs with an initial body weight (BW) of 25.5 kg (standard deviation = 2.5) were randomly assigned to one of 5 dietary treatments in a randomized complete block design with BW and sex as blocking factors to obtain 8 replicates and 4 pigs per pen. The low-oil corn DDGS contained 26.3% crude protein, 24.5% neutral detergent fiber, and 3.7% ether extract. Five experimental diets based on the corn, soybean meal, and wheat were formulated to contain 0%, 10%, 20%, 30%, and 40% of the low-oil corn DDGS for each of 2 phases.</p><p><strong>Results: </strong>During the overall period, average daily gain, average daily feed intake, and gain-to-feed ratio linearly decreased (p<0.05) as the low-oil corn DDGS inclusion rate increased. A one-slope broken-line analysis showed that the maximum inclusion rate of low-oil corn DDGS in swine diets without compromising gain-to-feed ratio was 16.5% during the overall period. The carcass characteristics were not affected by the inclusion of low-oil corn DDGS up to 40%. The firmness of loin and belly linearly decreased (p<0.05) as the low-oil corn DDGS inclusion rate increased. As the inclusion rate of low-oil DDGS increased, the saturated fatty acid content in both loin and pork belly linearly decreased (p<0.001), whereas the unsaturated fatty acid content linearly increased (p<0.001). The iodine value of lard also showed a linear increase (p<0.001) with increasing the low-oil corn DDGS inclusion rate.</p><p><strong>Conclusion: </strong>The maximum inclusion rate of low-oil corn DDGS in growing-finishing swine diets without detrimental effects on growth performance and pork quality was 16.5%.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"993-1002"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of gene CRTC2 on the differentiation of subcutaneous precursor adipocytes in goats.","authors":"Xuening Li, Tingting Hu, Ruiwen Li, Yanyan Li, Yaqiu Lin, Yong Wang, Wei Liu, Youli Wang","doi":"10.5713/ab.24.0248","DOIUrl":"10.5713/ab.24.0248","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to obtain goat CRTC2 gene sequence and elucidate its biological properties, and further study the impact of overexpression and interference of CRTC2 on the cell differentiation of goat subcutaneous precursor adipocytes.</p><p><strong>Methods: </strong>The sequence of goat CRTC2 was cloned by reverse transcription (RT)-polymerase chain reaction (PCR) and its molecular characterization was analyzed. The expression of CRTC2 gene in goat tissues and subcutaneous precursor adipocytes differentiated from 0 to 120 h was examined by quantitative real-time PCR (qRT-PCR). The effects of CRTC2 on the subcutaneous precursor adipocyte differentiation were investigated by using liposome transfection, Bodipy, Oil Red O staining and qPCR.</p><p><strong>Results: </strong>The results showed that the cloned goat CRTC2 gene was 2363 bp long (coding sequence [CDS] 2082 bp), encoding 693 amino acids. The relative expression levels of CRTC2 gene were highest in liver and then in kidney (p<0.05). During differentiation, the highest expression of CRTC2 in subcutaneous precursor adipocytes was observed at 120 of differentiating (p<0.01). In addition, we found that overexpression of CRTC2 significantly increased the expression of lipid metabolism-related genes (C/EBPα, C/EBPβ, PPARγ, DGAT1, DGAT2, ACC, FASN, SREBP1, AP2, LPL, ATGL) and promoted lipid accumulation. We then chemically synthesized goat CRTC2 small interfering RNA and transfected it into goat subcutaneous precursor adipocytes. The results revealed that SiRNA-mediated interference with CRTC2 significantly inhibited its differentiation and suppressed lipid droplet aggregation.</p><p><strong>Conclusion: </strong>So, this study indicates that CRTC2 is a positive regulator that promoting cell differentiation of subcutaneous adipocyte in goats, which lays the foundation for an in-depth study of the role of CRTC2 in lipid deposition in goats.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"873-883"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gender and age-related variations in rumen fermentation and microbiota of Qinchuan cattle.","authors":"Yueting Pan, Huaxuan Li, Juze Wang, Xiaolei Sun, Entang Liang, Juntao Guo, Jianfang Wang, Ke Zhang, Bingzhi Li, Mengqi Zan, Wucai Yang, Linsen Zan","doi":"10.5713/ab.24.0328","DOIUrl":"10.5713/ab.24.0328","url":null,"abstract":"<p><strong>Objective: </strong>Our study aimed to investigate the gender and age-related variations in rumen fermentation, serum metabolites, and microbiota in Qinchuan cattle.</p><p><strong>Methods: </strong>A total of 38 Qinchuan beef cattle were selected and maintained on a uniform diet for three months. Rumen fluid and blood samples were collected to determine rumen fermentation, serum metabolites, and microbial 16S rRNA sequencing.</p><p><strong>Results: </strong>The results revealed that the concentration of rumen butyrate in female Qinchuan cattle was significantly higher than in males (p<0.05). Isobutyrate, butyrate, and isovalerate exhibited significant age-related differences. Females exhibited lower serum glucose (GLU) and higher triglycerides (TG), nonesterifiedfatty acid (NEFA) levels compared to males (p<0.05). Serum albumin (ALB) and urea (UA) levels increased with age (p<0.05). Furthermore, the alpha diversity of rumen bacteria improved with age (p<0.05), with no gender differences observed. Males had higher relative abundances of Bacteroidota, Verrucomicrobiota, and Cyanobacteria, while females had higher Firmicutes and Desulfobacterota (p<0.05). The cellulose-degrading genus Ruminococcus and propionateproducing genus Succiniclasticum were more abundant in females, whereas the antiinflammatory genus Lachnospiraceae_NK4A136_group and the hemicellulose-degrading genus Prevotella were more abundant in males (p<0.05). Age-related differences in bacteria were found in Pseudobutyrivibrio and several members of the Lachnospiraceae. Functional prediction indicated that \"Amino acid metabolism\" and \"Lipid metabolism\" were mainly enriched in females, whereas \"Carbohydrate metabolism\" and \"Glycan biosynthesis and metabolism\" were enriched in males (p<0.05). RDA analysis highlighted butyrate as a key factor influencing the rumen bacterial community. NK4A214_group and Ruminococcus were positively correlated with butyrate, while Prevotella and Pseudobutyrivibrio were negatively correlated with butyrate (p<0.05).</p><p><strong>Conclusion: </strong>We observed a significant improvement in the diversity and stability of rumen microbiota as age increased. Ruminococcus, NK4A214_group, and Prevotella were likely contributors to variations in energy utilization and fat deposition between male and female Qinchuan cattle.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"941-954"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic polymorphisms in HMGCS1 gene and its association with slaughter characteristics, meat quality, and organ coefficients in Guizhou white goats.","authors":"Bo Zhou, Jiaqi Chen, Ziyang Li, Huan Liu, Jiali Xu, Houqiang Xu, Yong Ruan","doi":"10.5713/ab.24.0499","DOIUrl":"10.5713/ab.24.0499","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to identify polymorphisms in the gene encoding the 3-hydroxy-3-methylglutaryl-CoA synthase 1, HMGCS1, and analyze their association with slaughter characteristics, meat quality, and organ coefficients in Guizhou white goats.</p><p><strong>Methods: </strong>A total of 153 twelve-month-old Guizhou white goats (78 male and 75 female) were included in the study. Slaughter characteristics, meat quality, and organ coefficients were assessed. Association analyses between genotypes and phenotypic traits were conducted using a generalized linear model.</p><p><strong>Results: </strong>Four polymorphic loci were identified, i.e., g.15523T>C, g.15530G>C, g.18413T>C, g.19711G>A in exons 5, 8, and 9 of the HMGCS1 gene. Across all polymorphic loci, males of the same genotype generally exhibited significantly better slaughter traits compared to females of the same genotype (p<0.05). At the g.18413T>C locus, differences in shear force were observed between males and females of the same genotype and within the same sex across different genotypes (p<0.05). Organ coefficients were significantly higher in males of the same genotype compared to females (p<0.05). The g.15523T>C and g.15530G>C loci were found in strong linkage disequilibrium and significantly associated with intramuscular fat content (p<0.05). Fat content in diploid Hap2/2 individuals was significantly higher than in Hap1/1 and Hap1/2 (p<0.05). At the g.19711G>A locus, female goats with the CC genotype showed significantly higher levels of dry matter compared to male goats of the same genotype and female goats of other genotypes within the group. Organ coefficients for the liver and hooves in male goats were significantly higher than in females of the same genotype (p<0.05).</p><p><strong>Conclusion: </strong>The strongly linked loci g.15523T>C and g.15530G>C were significantly associated with intramuscular fat content and could be used as molecular markers for enhancing this trait in goat breeding programs, fostering the development of goat production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"910-928"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of subclinically hypocalcemic stress on the plasma metabolomic profile of dairy goats.","authors":"Jia Zhang, Dan Yang, Yafei Zeng, Kaini Guo, Jiajian Zhang, Yan Huang, Yuzhen Sui, Qimin Liu, Xiaoxuan Mo, Chenxu Zhao, Jianguo Wang","doi":"10.5713/ab.24.0567","DOIUrl":"10.5713/ab.24.0567","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to explore novel aspects of disease prevention and control in Guanzhong dairy goats through the application of metabolomics and lipidomics.</p><p><strong>Methods: </strong>In this study, plasma samples were collected from 96 primiparous Guanzhong dairy goats with similar body condition scores (2.75±0.15, mean±standard deviation) on the day of calving. The aim was to identify key differences in metabolite expression between diseased and healthy animals using metabolomics and lipidomics.</p><p><strong>Results: </strong>Twenty-three differential metabolites and 30 differentially altered lipids were identified, which were associated with various metabolic pathways, including phenylalanine and tyrosine metabolism, aminoacyl-tRNA biosynthesis, and glycerophospholipid metabolism. Our research revealed significant differences in the regulation of calcium-related hormones and associated metabolites between subclinical hypocalcemic and healthy dairy goats. Specifically, parathyroid hormone and aspartate aminotransferase were positively correlated in the healthy group and negatively correlated in the subclinical hypocalcemic group.</p><p><strong>Conclusion: </strong>The identification of phenylalanine and phosphatidylserine as potential biomarkers for subclinical hypocalcemia in dairy goats offers a novel approach to managing this condition, potentially transforming prevention and control strategies in the dairy goat industry.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"981-992"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}