Animal Bioscience最新文献

{"title":"Identification of key genes influencing carcass and meat quality traits in Neijiang and Large White pigs using gene co-expression analysis.","authors":"Patrick Kofi Makafui Tecku, Dong Chen, Kai Wang, Shixin Yu, Jiamiao Chen, Guoqing Tang","doi":"10.5713/ab.25.0259","DOIUrl":"https://doi.org/10.5713/ab.25.0259","url":null,"abstract":"<p><strong>Objective: </strong>The Neijiang indigenous pig breed of China and the Western Large White pig breed have unique meat quality and carcass characteristics. However, the genetic factors and mechanisms influencing their distinct meat and carcass traits are still not well understood. Therefore, using weighted gene co-expression network analysis (WGCNA), this study aimed to identify key genes influencing these traits.</p><p><strong>Methods: </strong>Transcriptome data from 17 Neijiang and 22 Large White pigs, along with their carcass weight, backfat thickness, eye muscle area, meat color, and muscle pH phenotypic data, were analyzed using WGCNA. A total of 9,249 genes were used to construct a weighted gene co-expression network.</p><p><strong>Results: </strong>Twenty-two co-expression gene modules were identified. Genes in the top modules were enriched in processes relevant to carcass and meat quality, such as protein transport. Further analysis identified six key genes, including HSPH1, HSPA4, DNAJA4, MRPL3, SEC63, and SRP54, for the Neijiang breed. Also, five key genes, consisting of EP300, SETD2, NIPBL, NAT10, and VCP, were identified for the Large White population. These genes were involved in biological processes related to mitochondrial function, protein targeting, chromatin organization, and morphogenesis.</p><p><strong>Conclusion: </strong>The findings from this study elucidate the regulatory mechanisms influencing the carcass and meat characteristics of the Neijiang and Large White pigs. The key genes could serve as potential biomarkers for enhancing breeding strategies aimed at improving pork quality.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of the DUSP6 Gene on the Proliferation and Differentiation of Porcine Subcutaneous Preadipocytes.","authors":"Xiang Xiang Yang, Xiao Han Sun, Zi Meng Du, Jun Dong Yi, Qiu Yan Wang, Ding Yin, Ya Lan Zhang, Xiao Ling Ding, Xian Rui Zheng, Xiao Dong Zhang, Zong Jun Yin, Yue Yun Ding","doi":"10.5713/ab.25.0175","DOIUrl":"https://doi.org/10.5713/ab.25.0175","url":null,"abstract":"<p><strong>Objective: </strong>Dual-specificity protein phosphatase 6 (DUSP6), also known as mitogen-activated protein kinase phosphatase 3 (MKP-3), was considered as a functional candidate gene for white fat accumulation in mice. However, the physiological function of the DUSP6 gene on white adipocyte adipogenesis in farm animals remains unknown. In this study, we aimed to clarify the effect of DUSP6 on porcine subcutaneous preadipocyte proliferation and differentiation.</p><p><strong>Methods: </strong>We first make clear that the patterns of DUSP6 expression is associated with fat contents in porcine fat deposition related tissues. Porcine subcutaneous preadipocytes were isolated and induced to differentiation. Small interfering RNAs were applied to deplete DUSP6. MTT assay, CCK-8 analysis, Oil Red O staining, triglyceride determination and quantitative real-time PCR were applied to study the regulatory role of DUSP6 during adipocyte adipogenesis in pigs.</p><p><strong>Results: </strong>We found that the expression levels of DUSP6 were significantly higher in backfat and longissimus dorsi tissues from fat-type pigs than in those from lean-type pigs. Consistently, the significantly induced expression of DUSP6 was also observed in differentiated adipocytes. In addition, knockdown of DUSP6 greatly inhibited preadipocytes proliferation, through the decreased cell viability and downregulated mRNA expressions of cell proliferation-associated genes, including PCNA, CDK1, CDK2. Furthermore, knockdown of DUSP6 significantly inhibited preadipocytes differentiation, as evidenced by markedly reduced lipid droplet formation, attenuated triglyceride accumulation and downregulated expression levels of adipogenic transcription masters (PPARγ、C/EBPβ、FASN and FABP4) in DUSP6 knockdown cells.</p><p><strong>Conclusions: </strong>Our results demonstrate that DUSP6 is required for white adipocyte adipogenesis in pigs.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effects of sea tangle water extract and various packaging methods on the product quality of pre-and post-rigor cured pork loin during storage.","authors":"Haeun Kim, Koo Bok Chin","doi":"10.5713/ab.25.0061","DOIUrl":"https://doi.org/10.5713/ab.25.0061","url":null,"abstract":"<p><strong>Objective: </strong>This experiment evaluated the physicochemical properties of low-salt cured pork after adding hot-water-extracted sea tangle (HWEST; 5%, 10%) to pre-rigor loin and packaging with vacuum packaging (VAC) or modified atmosphere packaging (MAP; 70% CO2, 20% N2) over an 8-wk storage period at 10°C.</p><p><strong>Methods: </strong>Pre-rigor cured loin (<1 h post-slaughter) treated with 0.8% salt and 5%/10% HWEST was prepared and compared to post-rigor cured loin with a salt level of 1.5%. The pH, color values, Warner-Bratzler shear value (kgf), lipid oxidation (thiobarbituric acid reactive substances), volatile basic nitrogen, cooking and purge loss (CL, PL %), expressible moisture (EM, %), and microbial counts of the cooked loin were measured.</p><p><strong>Results: </strong>MAP of cured pork loin yielded a lower PL value than VAC. In addition, lactic acid bacteria did not grow in MAP during storage. The 0.8%-salt pre-rigor cured loin treated with HWEST (5%, 10%) exhibited the same CL as the 1.5%-salt post-rigor cured loin. VAC elicited no differences in physicochemical properties between the 1.5%-salt post-rigor cured loin and HWEST-treated 0.8%-salt pre-rigor cured loin after the 8-wk storage period. However, the 0.8%-salt pre-rigor cured loin treated with HWEST (5%, 10%) yielded the same EM value as the 1.5%-salt post-rigor cured loin after 8 wk of VAC storage.</p><p><strong>Conclusion: </strong>The low salt content of meat products can be compensated for using pre-rigor cured loin, HWEST, and MAP.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiota profiles and antimicrobial resistance genes in sow fecal samples from farms with and without antibiotic use.","authors":"Sutthawongwadee Senawin, Chaiyapoom Bunchasak, Choawit Rakangthong, Chanwit Kaewtapee, Suporn Foongladda, Komwit Surachat, Rattapha Chinli, Wiriya Loongyai","doi":"10.5713/ab.25.0062","DOIUrl":"https://doi.org/10.5713/ab.25.0062","url":null,"abstract":"<p><strong>Objective: </strong>Antibiotics have been used in swine production, and they are known to be associated with the gut microbiota and antimicrobial resistance (AMR). This study aimed to evaluate the dynamics of the microbiota and AMR among fecal bacteria in sowsby 16S rRNA gene sequencing and TaqMan array card assays.</p><p><strong>Methods: </strong>A total of 40 healthy multiparous sows were tested in a completely randomized design. Sows were randomly divided into two groups: one was fed a commercial diet with antibiotics for 3 weeks from mating to day 21 of gestation, before the farrowing stage (amoxycillin 300 mg/kg and tiamulin 150 mg/kg: control group, ABO), and the other was fed the same diet without antibiotics (treatment group, NOABO).</p><p><strong>Results: </strong>The ABO group had a higher alpha diversity than the NOABO group (P < 0.05). The results re-vealed the highest bacterial abundance in the phylum Firmicutes in sow feces in the ABO group at an average level of 92.01% and 92.32% in the NOABO group. Erysipelotrichaceae, Clostridiaceae, and Terrisporobacter in the ABO group had enriched proportions. On the other hand, Lactobacillales, Bacilli, and Streptococcus were enriched in the NOABO group (P < 0.05). In terms of AMR, a comparison of the normal log of resistance gene copies between the ABO and NOABO groups displays that the gene copy number was significantly higher (P < 0.05) in the ABO group (59%) than in the NOABO group (41%) especially those of β-lactam, aminoglycosides, quinolones, and macrolides.</p><p><strong>Conclusion: </strong>Our investigations discovered that the core microbiota of withdrawal antibiotics may be related to the gut microbiota and AMR. Therefore, understanding the gut microbiota composition and function in animals could enable strategies for its modulation to improve sows' gut microbiota and minimize the negative impact of antibiotics.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of substituting corn meal with pellet feed from winged bean (Psophocarpus tetragonolobus) tuber in the diet of beef.","authors":"Pachara Srichompoo, Chaichana Suriyapha, Chanon Suntara, Sompong Chankaew, Metha Wanapat, Anusorn Cherdthong","doi":"10.5713/ab.25.0137","DOIUrl":"https://doi.org/10.5713/ab.25.0137","url":null,"abstract":"<p><strong>Objective: </strong>The experiment aimed to evaluate the effects of replacing corn meal with winged bean tuber (Psophocarpus tetragonolobus) pellets (WBTP) on energy utilization efficiency in Thai indigenous beef cattle.</p><p><strong>Methods: </strong>A 4 × 4 Latin square design was employed, with corn meal substituted by WBTP in the concentrate diet as the independent variable. The four replacement levels were 0%, 33%, 66%, and 100% dry matter (DM).</p><p><strong>Results: </strong>The intake of nutrients was not affected by replacing cornmeal with WBTP (p > 0.05). Substituting WBTP for corn meal did not significantly alter energy utilization (p > 0.05). Furthermore, the digestibility of nutrients did not significantly differ across the various WBTP replacement levels. Ruminal pH ranged from 6.73 to 7.04. Blood urea nitrogen values, ranging from 5.50 to 8.75 mg/dL, fell within the typical range for tropical ruminants. No significant differences were observed in total volatile fatty acid concentrations, which ranged from 84.56 to 101.87 mM (p > 0.05). Similarly, the proportions of acetate, propionate, and butyrate remained unchanged (p > 0.05), despite the incorporation of WBTP into the concentrate as a replacement for corn meal.</p><p><strong>Conclusion: </strong>This study highlights the potential of winged bean tuber pellets as a sustainable alternative to corn meal in the diets of Thai-native beef cattle. By replacing corn meal with WBTP at levels up to 100%, the study demonstrated no adverse effects on feed intake, nutrient digestibility, ruminal fermentation parameters, or energy efficiency.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Milk Composition Changes and Alterations in Bacteria, Serum, and Gut Metabolome Over Time in Lactating Naks and Simmental Cows.","authors":"Runze Wang, Xu Yunxiang, Allan Degen, Xuefeng Han, Xinsheng Zhao, Qunying Zhang, Yayu Huang, Bingqiang Bai, Yingkui Yang, Shujie Liu, Yanfeng Xue, Lizhuang Hao","doi":"10.5713/ab.25.0109","DOIUrl":"https://doi.org/10.5713/ab.25.0109","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to elucidate the mechanisms underlying milk composition divergence between naks (female yaks) and Simmental cows (S-cows) by integrating longitudinal multi-omics analyses of gut microbiota and metabolomes.</p><p><strong>Methods: </strong>We determined the gut microbiota and metabolites of both species over a 54-day period (day 26 to 80 of lactation), with ten naks and ten S-cows. Gut microbiota dynamics were assessed via 16S rRNA sequencing, while serum and fecal metabolomes were profiled using UHPLC-MS/MS. Statistical analyses included Wilcoxon rank-sum tests, LEfSe (LDA > 2, p < 0.05), and Spearman correlations (r > 0.70).</p><p><strong>Results: </strong>Milk yield was lesser (0.53-0.91 vs. 2.07-3.88 kg/d) but concentrations of fat (5.63-6.30% vs. 3.30-3.74%), protein (5.66-6.30% vs. 3.39-3.74%), and conjugated linoleic acid（CLA） (1.74-2.35% vs. 1.40-1.75%) were greater (p < 0.001) in nak than S-cow milk. Species-specific microbial signatures emerged. In naks, the g-Family-XIII-AD3011-group and g-norank-Ruminococcaceae were correlated with bile acid metabolism and CLA synthesis via 13-hydroxyoctadecadienoic acid transport. Additionally, the naks gut had a greater concentration of 13-hydroxyoctadecadienoic acid, a precursor of CLA, which may be transported to mammary cells via phosphatidylcholine and converted to CLA under the catalysis of fatty acid desaturase2(FADS2). S-cows harbored g-Succinivibrio and g-Eubacterium-ruminantium-group, which are linked to galactose utilization and mTOR-mediated amino acid allocation. Metabolomics revealed naks-enriched steroid biosynthesis and taurine pathways (FDR < 0.05), while S-cows exhibited a lactating network associated with greater milk yield.</p><p><strong>Conclusions: </strong>Host-specific gut microbiota mediated nutrient allocation trade-offs. Naks optimized lipid-rich milk through bile acid and CLA metabolic networks, whereas S-cows enhanced yield via microbial-galactose synergies. This research underscores the pivotal role of the gut microbiome in mediating milk composition and suggests that microbiome manipulation could be a promising strategy to enhance milk quality in ruminants.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spray-dried porcine plasma improves piglets' performance and modulates gut immune-related genes in the first week post-weaning.","authors":"Zacharia Waithaka Ng'ang'a, Núria Tous, Muzahir Hussain, Maria Ballester, Javier Polo, Raúl Beltrán-Debón, Joan Tarradas, David Torrallardona","doi":"10.5713/ab.25.0185","DOIUrl":"https://doi.org/10.5713/ab.25.0185","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to explore the effects of spray-dried porcine plasma (SDPP) in pre-starter feed of piglets on performance, fecal consistency, gut integrity biomarkers, and gene expression patterns related to intestinal function.</p><p><strong>Methods: </strong>Ninety piglets (18 pens; 5 pigs/pen; 8.16 ± 1.29 kg initial body weight) were offered pre-starter feed with either soy protein concentrate (control) or SDPP for 14 d post-weaning, and a common commercial starter feed between 14 and 35 d. Pigs' performance was recorded at 0, 7, 14 and 35 d of trial and their fecal consistency was assessed on the first 2 weeks. At 7 d, one piglet per pen (18 in total) was sampled for blood, intestinal mucosa, and bile. Blood plasma calprotectin and citrulline and bile sIgA were quantified with ELISA, and gene expression in the mucosa from ileum, jejunum, and caecum was analyzed with high-throughput microfluidic technology.</p><p><strong>Results: </strong>Relative to control, SDPP improved feed intake (215 vs 280 g/d; p<0.05), weight gain (133 vs. 205 g/d; p<0.05) and gain-to-feed ratio (0.61 vs. 0.74; p<0.05) between d 0 to 7. Plasma calprotectin and citrulline, and bile sIgA at d 7 were not affected. Piglets fed SDPP had higher expressions of IL-1β (p = 0.033) in jejunal mucosa, and of IL-1β (p = 0.018), IL-8/CXCL8 (p = 0.010), GBP1 (p = 0.014) and TGF-β1 (p = 0.015) in ileal mucosa, at 7 d post-weaning. No effects on fecal scores were observed.</p><p><strong>Conclusion: </strong>It is concluded that the administration of SDPP during the pre-starter phase improves piglet's performance during the first week post-weaning. These SDPP associated benefits appear to have been achieved through the modulation of gut homeostasis, potentially involving the regulation of inflammatory factors in the small intestinal mucosa.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of β-mannanase supplementation in low-energy and low-protein diets on performance, intestinal morphology, and fatty liver incidence in laying hens.","authors":"Kang Hyeon Kim, Eun Cheol Lee, Charline Mugeniwayesu, Tae Hyun An, Dong Yong Kil","doi":"10.5713/ab.25.0214","DOIUrl":"https://doi.org/10.5713/ab.25.0214","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the effect of dietary β-mannanase supplementation in low-energy and low-protein diets containing palm kernel meal and copra meal on productive performance, egg quality, intestinal morphology, and fatty liver incidence in laying hens.</p><p><strong>Methods: </strong>A total of four hundred 26-wk-old Hy-Line Brown laying hens were allotted to 1 of 5 dietary treatments with 8 replicates. The positive control (PC) diet was prepared with corn and soybean meal, whereas the negative control (NC) diet was formulated with decreased AMEn by 100 kcal/kg and CP by 0.85% than PC diets. High-mannan NC diet was also prepared by inclusion of 2.5% palm kernel meal and 2.5% copra meal, which was designed to contain energy and nutrient concentrations equal to those in the NC diet. Finally, dietary β-mannanase was supplemented to the high mannan NC diet at the levels of 0.05% and 0.10%.</p><p><strong>Results: </strong>Most productive performance and egg quality were not affected by dietary treatments. For jejunal morphology, villus height:crypt depth (VH:CD) ratio for hens fed PC diets or NC diets was greater (p<0.05) than those fed high-mannan NC diets, but supplementation of β-mannanase in high-mannan NC diets did not affect VH:CD ratio in hens. Hens fed NC diets had a greater (p<0.05) subjective color score in the liver than those fed PC diets or high-mannan NC diets supplemented with 0.05% and 0.10% β-mannanase.</p><p><strong>Conclusions: </strong>: Most productive performance and egg quality in laying hens were not affected by reduction in dietary energy and protein levels, inclusion of high-mannan ingredients, and dietary β-mannanase supplementation. No considerable benefits of dietary β-mannanase supplementation in low-energy and low-protein diets containing high-mannan ingredients on productive performance and health were observed in laying hens.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiRNA-24 Downregulates KLF6 Affecting STAT3 Protein Expression and Phosphorylation Regulating Melanogenesis in Cashmere Goat coat.","authors":"Baoyu Zhang, Runlai Liu, Yuxin Zhao, Xinyu Li, Huixian Su, Shufang Li, Jianping Li, Huaizhi Jiang, Qiaoling Zhang","doi":"10.5713/ab.24.0824","DOIUrl":"https://doi.org/10.5713/ab.24.0824","url":null,"abstract":"<p><strong>Objective: </strong>This study revealed how miRNA-24 downregulates KLF6, regulating melanogenesis in the Cashmere goat coat.</p><p><strong>Methods: </strong>The correlation between miRNA-24 and coat color was determined by Quantitative reverse transcription PCR (RT-qPCR). GO/KEGG analysis and bioinformatics tools were used to screen for target genes. Targeted interactions were confirmed using the dual luciferase assay. The expressions of miRNA-24 and KLF6 on different colored skins of Cashmere goats, and the KLF6 expression in B16-F10 transfected with miRNA-24 mimics/inhibitors/NC were detected by RT-qPCR, Western Blot, and Immunofluorescence. To investigate the pathway that KLF6 influenced, KLF6 was silenced by RNA interference (RNAi), and the melanogenesis pathway's gene expression was examined. The expression and phosphorylation of STAT3 protein in the pathway were inhibited. RNAi and Stattic were utilized to downregulate its protein expression and phosphorylation for detecting tyrosine expression. BALB/c mice were subcutaneously injected with antagomiRNA-24, a miRNA-24 inhibitor. And the influence of miRNA-24 downregulating KLF6 on melanogenesis was examined.</p><p><strong>Results: </strong>MiRNA-24 relates to coat color. KLF6 was chosen as the target gene, and the targeted interaction was confirmed. The expression trends of miRNA-24 and KLF6 were opposing in the skin. The mRNA and protein expression of KLF6 were decreased/increased in the cytoplasmic nucleus of B16-F10 transfected with miRNA-24 mimics/inhibitors. These suggest that KLF6 is downregulated by miRNA-24. When KLF6 was silenced, genes linked to the melanogenesis pathway, including WNT1, WNT2, PRKACA, MAPK1, TYR, and STAT3, were downregulated. When the protein and phosphorylation levels of STAT3 were suppressed, the mRNA level of TYR was decreased, suggesting that KLF6 influences TYR expression by mediating STAT3 expression and phosphorylation. BALB/c mice's skin injected with antagomiRNA-24 showed increased melanin content and decreased KLF6 expression.</p><p><strong>Conclusion: </strong>MiRNA-24 regulates melanogenesis in the Cashmere goat coat by downregulating the KLF6, mediating the STAT3 expression and phosphorylation.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of enzyme-treated soy oligopeptide on intestinal health, feed preference, and growth performance in nursery pigs.","authors":"Lan Zheng, Jung Yeol Sung, Sung Woo Kim","doi":"10.5713/ab.25.0153","DOIUrl":"https://doi.org/10.5713/ab.25.0153","url":null,"abstract":"<p><strong>Objective: </strong>The objective was to evaluate the effects of replacing soybean meal (SBM) with enzyme-treated soy oligopeptide (ESO) in nursery pig diets on intestinal health, growth performance, and feed preference.</p><p><strong>Methods: </strong>In Exp. 1, 128 pigs (average 5.2 kg) were housed in pens (4 pigs/pen), assigned to 4 diets supplemented with 0, 1, 2, or 3% of ESO, and fed for 32 d. On d 32, blood, and tissues and mucosa from the duodenum and jejunum were collected. In Exp. 2, 24 pigs (average 6.2 kg) were assigned to 6 pens (4 pigs/pen). Each pen had two separate feeders containing two diets with 0 or 2% of ESO for 27 d.</p><p><strong>Results: </strong>Increasing dietary ESO tended to quadratically change serum tumor necrosis factor-α (p=0.059; minimum at 1.6% of ESO) and villus height to crypt depth ratio (p=0.062; maximum at 1.8% of ESO). Increasing dietary ESO linearly reduced feed intake both linearly and quadratically (p<0.05) during phase 2 (maximum at 0.9% of ESO). Increasing dietary ESO both linearly and quadratically changed (p<0.05) weight gain during phase 2 (maximum at 1.0% of ESO). Increasing dietary ESO linearly changed (p<0.05) and tended to quadratically change (p=0.077) weight gain during the overall phase. Increasing dietary ESO tended to linearly change (p = 0.059) gain to feed ratio during phase 1 and quadratically change (p< 0.05) gain to feed ratio during phase 2 (maximum at 1.4% of ESO). Feed preference of the diet containing 2% of ESO was negatively associated with post-weaning days (R2 = 0.542).</p><p><strong>Conclusion: </strong>Gradually replacing soybean meal with enzyme-treated soy oligopeptide at levels from 1.4 to 1.8% enhanced intestinal health and gain to feed ratio, whereas exceeding 0.9 to 1.0% reduced feed intake and weight gain, suggesting that optimal level of enzyme-treated soy oligopeptide in nursery pig diets is 0.9%.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}