蛹虫草固体培养基对肉鸡生长性能、免疫指标和肠道健康的影响。

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Bioscience Pub Date : 2025-09-01 Epub Date: 2025-04-04 DOI:10.5713/ab.24.0791
Xiaoya Wang, Jingyan Zhang, Kang Zhang, Guowei Xu, Zhiting Guo, Xueyan Gu, Saad Ahmad, Liping Huang, Shuqi Liu, Jianxi Li, Lei Wang
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引用次数: 0

摘要

目的:研究蛹虫草固体培养基(CMM)对肉鸡生长性能、免疫指标、肠道健康和肠道菌群的影响。方法:选用320只雄性肉仔鸡,随机分为4组,每组8个重复,每个重复10只鸡。试验饲粮分为对照组、0.02% CMM组(低CMM)、0.2% CMM组(中CMM)和2% CMM组(高CMM)。分别于试验第14d和28d采集样品。采集血清进行生化指标和免疫球蛋白测定,观察十二指肠、空肠和回肠的肠道形态,采集盲肠内容物进行16S rRNA基因测序。结果:在28d的试验中,第14天和第28天,CMM组肉鸡的平均日增重(ADG)高于对照组,饲料转化率(FCR)低于对照组。与对照组相比,低CMM饲粮显著提高了胸腺和滑囊指数(P < 0.05)。ELISA结果显示,CMM组血清IgA、IgM和IgG水平升高,且低、中CMM组血清IgA水平显著高于对照组(P < 0.05)。低CMM饲粮增加VH和VH/CD的能力最强,CMM影响空肠组织炎症因子mRNA的相对表达水平。16S rRNA结果显示,CMM组在14d和28d时厚壁菌门(Firmicutes)的相对丰度和厚壁菌门/拟杆菌门(Bacteroidetes)的比值高于对照组,铁杆菌门(Deferribacteres)的丰度也有所增加(14d)。在属水平上,低CMM饲粮降低了拟杆菌纲(Bacteroides)丰度(P < 0.05),但提高了协同子纲(synerggistetes)丰度(P < 0.05)。结论:综上所述,饲粮中添加0.02% CMM可促进肉仔鸡生长性能,提高免疫力,改善肠道形态,调节肠道菌群。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effects of Cordyceps militaris solid medium on the growth performance, immunity parameters and intestinal health of broilers.

Effects of Cordyceps militaris solid medium on the growth performance, immunity parameters and intestinal health of broilers.

Effects of Cordyceps militaris solid medium on the growth performance, immunity parameters and intestinal health of broilers.

Effects of Cordyceps militaris solid medium on the growth performance, immunity parameters and intestinal health of broilers.

Objective: This study aimed to investigate the impact of Cordyceps militaris solid medium (CMM) on the growth performance, immunity parameters, intestinal health and gut microbiota of broilers.

Methods: A total of 320 male broilers were randomly allocated into 4 groups, consisting of 8 replicates and 10 birds per replication. The broilers were fed diets comprising the Control group, 0.02% CMM group (Low CMM), 0.2% CMM group (Mid CMM), or 2% CMM group (High CMM). Samples were collected on 14d and 28d of the experiment. Serum was collected for the determination of biochemical indices and immunoglobulins, the intestinal morphology of the duodenum, jejunum and ileum was observed, and cecum contents were collected for 16S rRNA gene sequencing.

Results: Over the course of the 28d trial, the broilers fed CMM had a higher average daily gain and lower feed conversion rate than the control broilers at Days 14 and 28. Notably, compared with those of the Control group, the Low CMM diet increased the thymus and bursa indices significantly (p<0.05). Enzyme-linked immunosorbent assay revealed that CMM increased the IgA, IgM, and IgG levels in the serum, and the IgA level in the Low and Mid CMM groups was significantly higher than that in the Control group (p<0.05). A Low CMM diet showed the strongest ability to increase villus height (VH) and villus height/crypt depth, and CMM affected the relative mRNA expression levels of inflammatory factors in jejunal tissue. The 16S rRNA results showed that the CMM group presented a greater relative abundance of Firmicutes and a higher Firmicutes/Bacteroidetes ratio than did the Control group at 14 d and 28 d, and the Deferribacteres abundance also increased (14 d). At the genus level, a Low CMM diet decreased Bacteroides abundance (p<0.05) but increased the abundance of Synergistetes (p>0.05).

Conclusion: These results illustrate that 0.02% CMM in the diet can promote growth performance, increase immunity, improve intestinal morphology, and regulate the intestinal flora of broilers.

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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
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223
审稿时长
3 months
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