Animal Bioscience最新文献

{"title":"Identification and characterization of microRNAs in the liver of yak (Bos grunniens) associated with energy deficiency at high-altitude.","authors":"Shuru Cheng, Fangping Shi, Zhixiong Tang, Feifei Yang, Quanlu Meng, Ting Liu","doi":"10.5713/ab.24.0756","DOIUrl":"10.5713/ab.24.0756","url":null,"abstract":"<p><strong>Objective: </strong>MicroRNAs (miRNAs) are small non-coding RNAs that regulate various biological processes including systemic metabolism and energy homeostasis. Comprehending hepatic functional adaptations is critical for yak (Bos grunniens) cattle as these herds typically suffer from malnutrition during the most of winter grazing seasons.</p><p><strong>Methods: </strong>To enhance the understanding of miRNA mediated post-transcriptional regulation in the context of energy deficiency in yaks, the miRNA transcriptomes of liver were profiled using deep sequencing analyses.</p><p><strong>Results: </strong>A total of 17,562,643 clean reads were acquired from a liver library, resulting in the identification of 369 known and 41 novel miRNA candidates. The top 10 miRNAs accounted for greater than 92% (9,854,350 counts) of the miRNAome, which were predicted to target 2,333 genes. Among the highly expressed miRNAs, miR-122 accounts for 57%, followed by bta-let-7f and bta-let-7a-5p. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses using DAVID bioinformatics resources indicated that the identified miRNAs targeted genes enriched in transcription and metabolism processes. Notably, 11 of the top 20 miRNAs were predicted to play a role in the regulation of lipid as well as glucose homeostasis via the PI3K-Akt interaction network.</p><p><strong>Conclusion: </strong>This is the first, at the best of our knowledge, liver miRNA-seq profiling analysis of moderate yak cows subjected to the combined effects of cold and nutritional stress at high altitude. Tissue specific miR-122 and liver enriched bta-let-7f represent two of the most abundant miRNAs found in the liver of yak cows. These findings enhance our understanding of the role of hepatic miRNAs in energy deficiency in yaks during winter grazing at high altitudes.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1372-1383"},"PeriodicalIF":2.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial variation and associated genes of total hair follicle density in goats.","authors":"Jipan Zhang, Ziyi Chen, Siyuan Zhang, Guoqiu Li, Xingqiang Fang, Yongju Zhao","doi":"10.5713/ab.25.0026","DOIUrl":"10.5713/ab.25.0026","url":null,"abstract":"<p><strong>Objective: </strong>Improving total hair follicle density (THFD) can directly result in increased fiber yield in cashmere goats. However, its phenotypic and genetic characteristics remain poorly understood. This study aimed to investigate the spatial distribution and associated genes of THFD in goats.</p><p><strong>Methods: </strong>A large-scale histological image analysis was conducted on 791 skin samples to characterize the spatial distribution of THFD at the local-site (n = 7), body-side (32 sites, n = 224), and whole-body (14 sites, n = 560) levels. Additionally, transcriptome sequencing was performed on 18 skin samples to identify genes associated with THFD.</p><p><strong>Results: </strong>The unique structure of the hair follicle group leads to an uneven distribution of hair follicles, resulting variation in THFD at the local-site. THFD exhibited a gradient pattern throughout the body, characterized by elevated values in the scapular region and decreased values in the abdominal region. THFD showed uneven distribution across the whole-body, with a consistent pattern observed in Inner Mongolia cashmere goats, Dazu black goats and their F1 hybrids (DBG♂ × IMCG♀). Through the integration of differential gene expression analysis, trend analysis, weighted correlation network analysis, Venn analysis, and literature review, four core genes (GJA1, DSP, CDH3, and PER1) were identified. These genes showed high and specific expression in skin tissue and demonstrated a significant response to changes in THFD.</p><p><strong>Conclusion: </strong>This study thoroughly characterized the spatial variation of THFD at the local-site, body-side, and whole-body levels in order to gain a better understanding of genetic characterization, associated genes and spatial variation. Additionally, we have identified four genes that are associated with THFD. These findings provide an important reference for the subsequent development and utilization of THFD genes in cashmere goats.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1356-1371"},"PeriodicalIF":2.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of two kinds of immunocastration vaccines and their immune effects on male goats.","authors":"Fuqiang Pan, Yumeng Guo, Panpan Cheng, Wei Qian, Mengdi Han, Qing Yi, Huihui Xie, Meng Cao, Yanqiuhong Li, Yuke Jia, Jiankun Cui, Xinbao Gong, Ziye Zhu, Fugui Fang, Yinghui Ling, Yunsheng Li, Jian Li, Ya Liu","doi":"10.5713/ab.24.0811","DOIUrl":"10.5713/ab.24.0811","url":null,"abstract":"<p><strong>Objective: </strong>Post-pubertal male goats exhibit undesirable behaviors (fighting, mounting) and reduced growth due to high testosterone, while traditional castration causes stress. Immunocastration offers a humane alternative. This study aimed to develop effective immunocastration vaccines.</p><p><strong>Methods: </strong>The gonadotropin-releasing hormone (GnRH) octamer vaccine (G8), luteinizing hormone receptor (LHR) and GnRH octamer tandem vaccine (LG) were developed and synthesized for this study. Forty 3-month-old male goats were randomly divided into four groups, and subjected to different treatments: surgical castration (SC group), immunization with the G8 vaccine (G8 group), immunization with the LG vaccine (LG group), or left intact (non-castration [NC] group). After the first immunization, serum antibodies and testosterone levels, as well as body weight, body size, and scrotal size, were measured at various time points. Testicular size and slaughter rate were measured at the time of slaughter, 20 weeks after the first immunization.</p><p><strong>Results: </strong>Both vaccines effectively elicited the corresponding antibodies in male goats; the testosterone levels in the G8 and LG groups were significantly reduced compared to the NC group (p<0.01). Four weeks after the first immunization, this trend persisted throughout the experiment; the testicular organ index and size of the G8 group were significantly (p<0.05) smaller than those of NC group at slaughter. In comparison to the NC group, the seminiferous tubule diameter in the G8 and LG groups was significantly reduced (p<0.01), accompanied by a notable decrease in Leydig Cells and various stages of spermatogenic cells. Additionally, the weight gain of goats in the SC group was significantly lower than that of other groups two weeks after the first immunization (p<0.05).</p><p><strong>Conclusion: </strong>The two immunocastration vaccines developed in this study effectively inhibit the testicular development and spermatogenesis in male goats, leading to a reduction in testosterone levels.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1411-1421"},"PeriodicalIF":2.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA ST6GALNAC3 inhibits dermal fibroblast proliferation and migration in cashmere goat hair follicles via chi-miR-24-3p/ID4 axis.","authors":"Rong Ma, Qing Ma, Lu Zhang, Bingjie Ma, Xuxu Bao, Yiming Zhang, Le Wang, Qi Lv, Zhiying Wang, Ruijun Wang, Rui Su, Yanhong Zhao, Fangzheng Shang, Yu Wang, Yanjun Zhang","doi":"10.5713/ab.25.0115","DOIUrl":"https://doi.org/10.5713/ab.25.0115","url":null,"abstract":"<p><strong>Objective: </strong>Dermal papilla is formed by the continuous proliferation and differentiation of dermal fibroblasts, which is the key to the normal development of hair follicles. Therefore, this study aims to elucidate the role of lncRNA ST6GALNAC3, which is significantly differentially expressed during the secondary hair follicle development stage in cashmere goats, on dermal fibroblasts, and to thoroughly analyze the regulatory mechanism of this lncRNA.</p><p><strong>Methods: </strong>Taking into account the characteristics of secondary hair follicle development, we conducted a screen for lncRNAs that are associated with this process. CCK8, EdU, and flow cytometry detected the effects of lncRNA ST6GALNAC3 on cell proliferation and migration. Subsequently, we employed bioinformatics analysis to predict the target miRNAs of lncRNA ST6GALNAC3 and the target genes of these miRNAs, respectively, and initially constructed the regulatory axis of lncRNA ST6GALNAC3-chi-miR-24-3p-ID4. Subsequently, luciferase reporter assays and rescue experiments were performed to confirm the regulatory axis at both molecular and cellular levels, thus elucidating the mechanism by which lncRNA ST6GALNAC3 regulates dermal fibroblasts.</p><p><strong>Results: </strong>A total of 158 lncRNAs related to secondary hair follicle morphogenesis were identified. Among them, lncRNA ST6GALNAC3 was significantly differentially expressed on embryonic day 75 and significantly inhibited the proliferation and migration of dermal fibroblasts. The results showed that lncRNA ST6GALNAC3 could target chi-miR-24-3p, and chi-miR-24-3p could target the ID4 gene. Subsequently, the results of luciferase reporter assay and rescue assay showed that chi-miR-24-3p had binding sites with both lncRNA ST6GALNAC3 and ID4, and lncRNA ST6GALNAC3 could indirectly regulate the proliferation and migration of dermal fibroblasts through chi-miR-24-3p/ID4 axis.</p><p><strong>Conclusion: </strong>LncRNA ST6GALNAC3 inhibits the proliferation and migration of dermal fibroblasts through the chi-miR-24-3p/ID4 axis, thus suppressing the formation of dermal papilla structures and influencing the morphogenesis of secondary hair follicles during the embryonic period.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of circRNA characteristics and identification of key circRNAs in the hypothalamus during sexual maturation in female goats.","authors":"Qing Li, Jian-Min Wang, Yanyan Wang, Peipei He, Lu Zhang, Tianle Chao","doi":"10.5713/ab.25.0275","DOIUrl":"https://doi.org/10.5713/ab.25.0275","url":null,"abstract":"<p><strong>Objective: </strong>Precocious puberty can shorten reproductive cycles, enhance reproductive capacity, and reduce feeding costs. Consequently, precocious livestock are widely utilized in cross-breeding. This study aims to elucidate the key molecular mechanisms by which circular RNA (circRNA) regulates the sexual maturation of sexually precocious goats.</p><p><strong>Methods: </strong>In this study, we analyzed the circRNA expression profiles of hypothalamic tissue from Jining grey goats at four distinct postnatal developmental stages (1 day, 2 months, 4 months, 6 months).</p><p><strong>Results: </strong>A total of 23,993 circRNAs were identified across these stages, predominantly derived from exonic regions, with 1,052 circRNAs exhibiting differential expression. Additionally, competitive endogenous RNA (ceRNA) analysis suggested that novel_circ_0002274/chi-miR-197-5p/ estrogen receptor 1 (ESR1) and novel_circ_0002274/chi-miR-30c-3p/ oxytocin/neurophysin I prepropeptide (OXT) may regulate sexual maturation in goats via the estrogen signaling pathway and the oxytocin signaling pathway.</p><p><strong>Conclusion: </strong>This study contributes to understanding the function of circRNAs in hypothalamic regulation of sexual maturation and provides valuable insights for breeding superior goat breeds.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary starch and protein contents on lactation performance, blood metabolites and methane production of Nili Ravi buffaloes.","authors":"Hina Tahir, Saima Naveed, Nisar Ahmad, Burhan E Azam, Muhammad Asim Tausif, Sundas Qamar, Saba Anwar, Muhammad Binyameen, Ijaz Hussain, Mubashar Ali, Muhammad Uzair Akhtar, Muhammad Naveed Ul- Haque","doi":"10.5713/ab.25.0074","DOIUrl":"https://doi.org/10.5713/ab.25.0074","url":null,"abstract":"<p><strong>Objective: </strong>Imbalanced feeding of protein and energy in ruminants coupled with increasing concerns over low production efficiencies and high livestock emissions intensify the feeding challenges, especially in buffaloes. This study was planned to evaluate the independent or interactive effects of dietary starch and crude protein (CP) on lactation performance, blood metabolites, and methane production of dairy buffaloes.</p><p><strong>Materials and methods: </strong>Sixteen lactating multiparous Nili Ravi buffaloes received the following dietary treatments of low (LS) or high starch (HS) content combined with low (LP) or high protein (HP) content in a 4 × 4 Latin square design: 1) LSLP, 2) LSHP, 3) HSLP, and 4) HSHP. Dietary starch contents were 19.5 and 28.7% in LS and HS diets, whereas, CP contents were 8.8 and 10.6% in LP and HP diets, respectively.</p><p><strong>Results: </strong>Although milk yield was not affected by dietary treatments, the HP diet increased milk protein and lactose contents compared with LP diet in HS group, however, no such increase was observed in LS group, resulting in a starch × CP interaction. Efficiencies of metabolizable protein and milk nitrogen decreased with HP diet compared with LP diet. Metabolic efficiency of metabolizable protein was higher in buffaloes fed LS diet compared with HS diet. Rumen pH decreased with HS diet compared with LS diet in HP group, however, no such change was observed in LP group. Methane production was increased with LS diet compared with HS diet. Contrarily, methane production was decreased in buffaloes fed HP diet compared with those fed LP diet.</p><p><strong>Conclusion: </strong>These findings indicated that dietary starch interacted with CP level for milk protein, milk lactose, rumen pH, and methane production of lactating buffaloes. Overall, high starch combined with high protein content can effectively improve milk protein and lactose contents while reducing the methane emission of lactating buffaloes.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential use of insect protein and phytonutrient based tropical plant supplementation on rumen fermentation characteristics and microbial protein synthesis in Thai native beef cattle.","authors":"Burarat Phesatcha, Kampanat Phesatcha, Maharach Matra, Thiwakorn Ampapon, Metha Wanapat","doi":"10.5713/ab.25.0166","DOIUrl":"https://doi.org/10.5713/ab.25.0166","url":null,"abstract":"<p><strong>Objective: </strong>This experiment evaluated the use of the insect protein and phytonutrient based tropical plant supplementation on rumen fermentation characteristics and microbial protein synthesis in Thai native beef cattle. Enhancing protein utilization and promoting rumen fermentation could be achieved by combining high-protein crickets and phytonutrients from mangosteen peel and lemongrass powder to pellet (CMLP).</p><p><strong>Methods: </strong>Four native male Thai beef cattle were randomly assigned treatments using a 4×4 Latin square design to receive four dietary treatments. The treatments were as follows: control (no supplementation), CMLP supplement at 50 g/h/d, CMLP supplement at 100 g/h/d and CMLP supplement at 150 g/h/d, respectively.</p><p><strong>Results: </strong>Results revealed that the supplementation of CMLP in beef cattle did not influence on rice straw intake, concentrate intake and total feed intake, which also enhanced the digestibility of crude protein (CP), acid detergent fibre (ADF) and neutral detergent fibre (NDF) (p<0.05). Additionally, volatile fatty acid production of propionate and bacterial population were increased (p<0.05), protozoal populations and production of methane decreased (p<0.05) with a higher level of CMLP supplementation. Furthermore, efficiency of microbial nitrogen synthesis (EMNS) significantly increased by increasing the level of CMLP supplementation, particularly at 150 g.</p><p><strong>Conclusion: </strong>Our results suggested that CMLP supplementation, particularly at 150 g/h/d, enhanced nutrient digestibility, increased the propionic acid proportion, and promoted microbial protein synthesis while reducing protozoal populations and methane production. CMLP showed promise as an effective dietary protein supplement that improved rumen fermentation and performance of Thai native beef cattle.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional regulation of porcine PABPN1 gene in adipogenesis.","authors":"Rongru Zhu, Xuelian Zhao, Minghang Chang, Siqi Yang, Xiaohan Zhang, Yingke Liu, Zhigang Gu, Xiu-Qin Yang","doi":"10.5713/ab.25.0035","DOIUrl":"https://doi.org/10.5713/ab.25.0035","url":null,"abstract":"<p><strong>Objective: </strong>This study was designed to reveal the transcriptional regulatory mechanism and effects of poly(A)-binding protein nuclear 1 (PABPN1)on adipogenesis and the polymorphisms as well.</p><p><strong>Methods: </strong>Transcription factors were identified with dual-luciferase reporter assay, overexpression, site-directed mutagenesis, real-time quantitative PCR (qPCR), electrophoretic mobility shift assay and Chromatin immunoprecipitation-qPCR. Preadipocyte differentiation was measured with gain- and loss-of-function, Oil Red O staining and extraction assays. Single nucleotide polymorphisms (SNPs) were identified with direct sequencing of PCR products in the promoter, and the effects of SNPs on PABPN1 expression were identified with dual-luciferase reporter assay.</p><p><strong>Results: </strong>Both CCAAT/enhancer-binding protein (C/EBP) α and β can regulate the expression of PABPN1 by directly binding to the promoter. PABPN1 promotes the preadipocyte differentiation in pigs. A total of three SNPs were identified, and the haplotype mutation, Haplotype GCC, significantly increases the promoter activity of PABPN1.</p><p><strong>Conclusions: </strong>PABPN1 promotes the preadipocyte differentiation as a downstream gene of C/EBP α and β. Haplotype GCC has the potential as a molecular marker for selecting fat trait in pigs.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary sodium butyrate supplementation on fat metabolism in lamb adipose and liver tissues.","authors":"Yue Zhang, Hongbo Qu, Yueying Guo, Mirco Corazzin, Min Zhang, Ting Liu, Lin Su, Lihua Zhao, Lina Sun, Ye Jin","doi":"10.5713/ab.24.0919","DOIUrl":"https://doi.org/10.5713/ab.24.0919","url":null,"abstract":"<p><strong>Objective: </strong>Sodium butyrate (SB) is a potentially useful feed additive, however, its effects on lipid metabolism in adipose and liver tissues of lambs are still not fully explored. This study systematically examined the effects and underlying mechanisms of dietary SB supplementation on lipid metabolism in lamb adipose and liver tissues from an adipose-blood-liver-perspective.</p><p><strong>Methods: </strong>Twelve 3-month-old male lambs (22.37 ± 2.05 kg) were randomly divided into a control group and a SB group. The adipose tissue cellular morphology and lipid metabolism-related indices in both adipose and liver tissues were measured.</p><p><strong>Results: </strong>The results indicated that SB significantly reduces abdominal and perirenal adipose tissue mass, as well as in the average area and diameter of adipocytes (P < 0.05). Dietary supplementation with SB activated adenosine 5'-monophosphate -activated protein kinase α1 (AMPKα1) in lamb adipose tissue, resulting in upregulated mRNA expression of hormone-sensitive triglyceride lipase (HSL) and downregulated mRNA expression of sterol regulatory element-binding protein 1 and fatty acid synthase (P < 0.05). Simultaneously, adiponectin secretion and receptor expression in adipose tissue, as well as serum adiponectin levels, were significantly elevated (P < 0.05). Moreover, dietary supplementation with SB increased the levels of TCA cycle metabolites in lamb liver, including oxaloacetate, citrate, cis-aconitate, and succinate (P < 0.05), while simultaneously activating the liver AMPKα1 signaling pathway. This led to upregulated HSL, platelet glycoprotein 4, and long-chain acyl-CoA synthetase mRNA expression (P < 0.05), thereby enhancing liver fatty acid metabolism.</p><p><strong>Conclusion: </strong>In summary, dietary supplementation with SB alters adiponectin levels in lambs, activates the AMPK signaling pathway, promotes adipose tissue lipolysis, and regulates liver lipid metabolism. The findings provide valuable insights into the use of SB for managing lamb body fat reserves and offer a robust basis for further research in animal bioscience.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microsatellite analysis revealed the genetic diversity and population structure of 18 native black goat breeds in China.","authors":"Tao Zhang, Jiaxue Guo, Ge Qin, Guangxin E, Deli Huang, Yan Zeng, Yongju Zhao, Zhongquan Zhao, Yongfu Huang, Yanguo Han","doi":"10.5713/ab.25.0224","DOIUrl":"https://doi.org/10.5713/ab.25.0224","url":null,"abstract":"<p><strong>Objective: </strong>In China and Southeast Asia, black goats command higher selling prices. However, the blind breeding practices carried out by farmers pose a threat to the original genetic diversity of the population. Therefore, the objective of this study is to conduct a systematic detection of the genetic diversity of native black goat breeds, aiming to provide a reference for the protection and improvement of these valuable native black goat breeds.</p><p><strong>Methods: </strong>Genetic diversity and population structure of 18 black goat breeds were estimated by utilizing 16 microsatellite markers. Subsequently, data analysis was carried out with the assistance of software like Phylip, Fstat, Arlequin, Structure. For the purpose of visualization, ITOL and Structure Selector were used to present the results in a visual manner.</p><p><strong>Results: </strong>The mean number of alleles (MNA) per population ranged from 4.75 to 9.56, with an average of 6.38. The observed heterozygosity (HO) of each breed ranged from 0.46 to 0.68, all of which were lower than the expected heterozygosity (HE). The inbreeding coefficient (FIS) of the 18 breeds ranged from -0.003 to 0.376. Among them, the FIS values of Meigu goat (MG), Yimeng black goat (YM), Yunling goat (YL), Guizhou black goat (GZ) and Ziwuling black goat (ZWL) were significantly higher than those under random rearrangement (p < 0.05). All pairwise Fixation index (FST) between the Chinese black goat populations reached a significant level (p < 0.05). Finally, the results of Bayesian model-based clustering and a neighbor-joining tree based on Nei's genetic distance showed these eighteen breeds can be further classified into seven genetic clusters.</p><p><strong>Conclusion: </strong>All breeds showed high genetic diversity. MG had excessive inbreeding, and CZ and LZ were at risk of losing original genetic traits. Similar geographical and climatic conditions might lead to similar genetic materials in different breeds.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}