Animal Bioscience最新文献

{"title":"Effects of saturated fat supplementation and forage-neutral detergent fiber content on production performance of lactating buffaloes.","authors":"Saba Anwar, Anjum Khalique, Hifzulrahman, Muhammad Naeem Tahir, Muhammad Asim Tausif, Burhan E Azam, Hina Tahir, Sundas Qamar, Muneer Ahmad Khan, Ijaz Hussain, Muhammad Naveed-Ul-Haque","doi":"10.5713/ab.24.0760","DOIUrl":"10.5713/ab.24.0760","url":null,"abstract":"<p><strong>Objective: </strong>The study aimed to find out the effects of dietary forage-neutral detergent fiber (fNDF), saturated fat, and their interaction on milk production, composition, and blood metabolites in lactating buffaloes.</p><p><strong>Methods: </strong>Sixteen multiparous buffaloes received 4 treatments with 2 different levels of fNDF and saturated fat according to a changeover design with 6-wk periods under restricted feed intake. Within each fNDF level, the buffaloes received 1 of the 2 saturated fat levels for 3-week subperiods, which corresponded to the following treatments: 1) 23.4% fNDF and 0% fat, 2) 23.4% fNDF and 2% fat, 3) 33.5% fNDF and 0% fat and 4) 33.5% fNDF and 2% fat.</p><p><strong>Results: </strong>Increasing fNDF levels increased the body condition score (BCS), body weight (BW), and rumen pH. Despite these changes, levels of fNDF did not alter the milk components and yield. Dietary saturated fat supplies improved milk fat content and tended to enhance the yields of milk fat and fat-corrected milk. In addition, the saturated dietary fat supplies increased BW, milk nitrogen efficiency, and cholesterol concentration, and decreased predicted methane yield. However, the milk yield, BCS, and rumen pH remained unaffected by dietary fat supplies. A high fNDF diet with dietary saturated fat supplementation tended to increase the milk fat content.</p><p><strong>Conclusion: </strong>Balancing diets with saturated fats and fiber significantly affects productivity. These results indicate that feeding fNDF with saturated fat may be a viable option for producers to improve milk fat production, enhance body condition, and reduce methane production in buffaloes.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1906-1914"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taking intestinal microbes and the immune system as opportunities to maintain the intestinal health of piglets: review.","authors":"Xiaohong Hou, Yue Chen, Fengming Chen, Yulong Yin, Kang Xu","doi":"10.5713/ab.25.0100","DOIUrl":"10.5713/ab.25.0100","url":null,"abstract":"<p><p>Numerous commensal microbes colonize in the pig gut, while the microbiota gets benefits from the gut environment, it additionally assists significantly with the host's development, metabolism, and immunity. The immune system can regulate the homeostasis of gut microorganisms by secreting a variety of effector factors. Similarly, gut microorganisms can also regulate the intestinal immune system via specific components or metabolites. Despite their close relationship, microbes and immune cells have their own individual ecological niche in the gut. Microbes are confined to the intestinal lumen, while immune cells are located in the intestinal tissues, and the mechanisms of how they interact with each other to promote intestinal health have not yet been thoroughly investigated. This article focuses on the different mechanisms by the interaction between intestinal immune system, gut microorganisms and microbial metabolites to regulate the intestinal health of piglets, and discusses the strategies to enhance the intestinal health of piglets through dietary interventions in order to provide theoretical support promoting the healthy growth of piglets.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1827-1840"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid determination of nutrient composition and mineral element content of common vetch (Vicia sativa L.) using near-infrared spectroscopy.","authors":"Tao Wang, Li Wang, Tao Guo, Yanli Shi, Baocang Liu, Fei Li","doi":"10.5713/ab.24.0872","DOIUrl":"10.5713/ab.24.0872","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to establish an accurate and reliable near-infrared spectroscopy to enable rapid, efficient, and non-destructive evaluation of the nutritional quality of common vetches across different regions and varieties.</p><p><strong>Methods: </strong>A total of 190 samples from various regions and varieties were selected for this study, which were divided into a calibration set (4:1 ratio) and validation set. The original spectrum of the calibration set is subjected to 10 different pretreatment techniques in combination with first and second derivatives, while the prediction model was established by combining the measured value of common vetch with the modified partial least squares method.</p><p><strong>Results: </strong>The results indicate that the calibration root-mean-square error and cross-validation root-mean-square error values range from 0.01 to 1.25 and 0.01 to 1.37, respectively. The determination coefficients of cross-validation (R2 CV) for phosphorus (P), potassium (K), magnesium (Mg), and iron (Fe) are relatively low at 0.82, 0.86, 0.82, and 0.74, respectively; however, all other indicators have R2 CV values above 0.90. The predicted root means square errors (RMSEP) for common vetch indexes range from 0.01 to 1.87, with RMSEP values higher than 1.0 observed for crude protein, neutral detergent fiber, acid detergent fiber, and ash indices, whereas RMSEP values lower than or equal to 1.0 were obtained for other indicators. The measured coefficient of determination (R2p) demonstrates that the R2p values for each nutrient element and mineral element vary from 0.70 to 0.96. The residual prediction deviation (RPD) values for Mg exhibit relatively low levels, while the RPD values for other indicators exceed 2.0.</p><p><strong>Conclusion: </strong>These findings suggest that this study provides a viable approach to evaluate the nutritional composition and mineral element content of different varieties and regions of common vetch.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1921-1933"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing the breeding strategy in Vanaraja female parent line chicken by monitoring the genetic variability for the major performance traits.","authors":"Niranjan Matam, Rudra Nath Chatterjee, Aneet Kour, Leslie Leo Lawrence Prince, Santosh Haunshi, Rajkumar Ullengala","doi":"10.5713/ab.25.0304","DOIUrl":"https://doi.org/10.5713/ab.25.0304","url":null,"abstract":"<p><strong>Objectives: </strong>Vanaraja is a highly successful backyard chicken variety developed by crossing males of Vanaraja male line (PD-1) with females of Vanaraja female line (PD-2). Vanaraja was improved over the generations through selection in parent lines. This study was designed to estimate the genetic parameters and trend for major traits in PD-2 line in order to determine the future breeding strategy in the population.</p><p><strong>Methods: </strong>Seven generations data of PD-2 population was utilized to precisely examine the existing additive genetic variability for the major growth and production traits. Egg mass at 52 weeks of age (EM52) is the primary selection trait. The best-fitted model for each trait was utilized out of the six models to obtain the genetic parameters. Subsequently, estimated breeding values were used to plot genetic trend for the major production traits.</p><p><strong>Results: </strong>Models with maternal genetic and permanent environmental effects were identified as the best-fitted for most of the body weight traits. Model 1 with direct additive effect only as random effect was adjudged as the best for the primary selection trait EM52. EM52, was low heritable with the estimates as 0.13±0.03. The average estimated breeding value of EM52 increased linearly and significantly as a direct response to selection in PD-2 line and was 0.47 kg after seven generations of selection.</p><p><strong>Conclusion: </strong>Based on the findings, PD-2 population still has sufficient genetic variability for the selection trait and other performance traits. The present breeding program can continue in order to produce genetic improvement in the Vanaraja chicken.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of Growth Performance and Health Status in Heat-Stressed Growing Rabbits Using Alpha-Lipoic Acid-Loaded Chitosan Nanoparticles.","authors":"Ali Ali El-Raghi, Huda A Al Doghaither, Ayat B Al-Ghafari, Mahmoud A E Hassan, Reham A Elbhnsawy, Naglaa A Gomaa, Ekramy M Elmorsy","doi":"10.5713/ab.25.0456","DOIUrl":"https://doi.org/10.5713/ab.25.0456","url":null,"abstract":"<p><strong>Objective: </strong>This study is the first to study the effect of Alpha-lipoic acid-loaded chitosan nanoparticles (ALA-CHNPs) on growth performance, feed utilization, and health in heat-stressed growing rabbits.</p><p><strong>Methods: </strong>A total of one hundred healthy 5-week-old rabbits were randomly divided into four groups of 25, each receiving diets supplemented with 0, 100, 200, or 400 mg ALA-CHNPs per kilogram.</p><p><strong>Results: </strong>The dietary supplementation significantly improved growth performance, feed conversion ratio, physiological responses, liver weight, and dressing percentage. Supplementation with ALA-CHNPs resulted in a linear reduction in liver enzymes activities, as well as in the levels of total bilirubin, hydrogen peroxide, glutathione, catalase, superoxide dismutase, immunoglobulin G, lysozyme activity, interleukin-6 and -10. No significant differences were observed between the ALA-CHNPs200 and ALA-CHNPs400 groups (p>0.05).The levels of blood serum total protein, albumin, globulin, total antioxidant capacity, glutathione peroxidase activity, immunoglobulin A and M, and nitric oxide showed a quadratic increase, peaking at ALA-CHNPs doses ranging from 250-300 mg/kg diet. Conversely, the concentrations of triglycerides, cholesterol, glucose, malondialdehyde, interferon-gamma, tumor necrosis factor-alpha, and nuclear factor kappa B exhibited a quadratic decrease, with optimal reductions occurring at doses between 250-300 mg/kg diet. Supplementation with ALA-CHNPs reduced liver damage caused by heat stress, restoring normal hepatic morphology. The hydrophobic interactions of ALA with antioxidant enzymes and cytokines helped mitigate heat stress-induced oxidative stress.</p><p><strong>Conclusion: </strong>adding 250-300 mg ALA-CHNPs/kg diet enhanced growth performance, redox balance, immune function, and inflammatory response in fattened rabbits raised during the summer.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination and prediction of amino acid digestibility in rice bran for growing pigs.","authors":"Rui Li, Hui Tang, Menglong Deng, Xianji Jiang, Ganyi Feng, Xiaojie Liu, Qing Ouyang, Mingzhou Tian","doi":"10.5713/ab.25.0280","DOIUrl":"https://doi.org/10.5713/ab.25.0280","url":null,"abstract":"<p><strong>Objective: </strong>This experiment was conducted to determine the apparent ileal digestibility (AID) or standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in 10 rice brans fed to pigs, and to construct predictive models for the SID of CP and AA based on the chemical composition of rice bran.</p><p><strong>Methods: </strong>Twenty-two healthy [Duroc × (Landrace × Yorkshire)] pigs equipped with ileal T-cannulas (initial body weight: 30 ± 1.5 kg) were selected for this experiment. A replicated 11 × 3 incomplete Latin square design was adopted. The experiment consisted of 3 periods each lasting for 7 days and had 11 dietary treatment groups. The diets included 10 rice bran diets as well as a nitrogen-free diet for determining endogenous losses of AA, and each treatment group had 6 replicates. Titanium dioxide was added to each diet as an indicator at a concentration of 0.3%.</p><p><strong>Results: </strong>Except for dry matter (DM) and total phosphorus (TP), the coefficients of variation for the chemical components among 10 rice bran sources were all greater than 10%. The SID of CP, Lys, Met, Thr, and Trp in rice bran was 81.89% ± 6.23, 80.33% ± 2.21, 83.25% ± 5.51, 79.89% ± 5.68, and 72.12% ± 4.71, respectively. The best prediction equations for the SID of CP and four limiting AA in rice bran are as follows: SIDCP=974.032-29.805TP-9.496DM (R2=0.88), SIDLys=471.278-9.267TP-4.245DM+1.401dummy variable (R2=0.92), SIDMet=122.281-2.712CP (R2=0.51), SIDThr=51.864+2.204GE-1.324CF [(R2=0.97); GE, gross energy; CF, crude fiber], and SIDTrp= 73.912-23.268Calcium-0.0.336NDF+0.318TS [(R2=0.96); NDF, neutral detergent fiber; TS, total starch].</p><p><strong>Conclusion: </strong>There was significant variability among the chemical components of 10 different sources of rice bran. Moreover, GE, DM, CP, TP, TS, CF, and NDF could serve as crucial predictors for the SID of AA.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population structure and genome-wide association study of body conformation traits of two native goat breeds in China.","authors":"Rong Yang, Di Zhou, Yanli Lv, Xingzhou Tian, Liqun Ren, Fu Wang, Zhengang Guo, Yongju Zhao, Jipan Zhang","doi":"10.5713/ab.25.0334","DOIUrl":"https://doi.org/10.5713/ab.25.0334","url":null,"abstract":"<p><strong>Objective: </strong>Body conformation traits have a direct impact on carcass performance in the meat goat industry. This study explored the population genetics of two Chinese goat breeds and identified the genomic variates contributing to their body conformation traits.</p><p><strong>Methods: </strong>The Guizhou black goat (GBG, n=104) and Hezhang black goat (HBG, n=100) were genotyped by whole-genome sequencing and phenotyped by measuring their body height (BH), body length (BL), chest depth (CD), chest width (CW), chest girth (CG), rump width (RW), rump height (RH), and cannon circumference (CC).</p><p><strong>Results: </strong>The relatedness analysis showed that these goats were low genetically kinship-related, with the GBG and HBG being relatively independent but with some genetic introgression. The lambda values showed that the GWAS model was reliable, and a total of 33, 1, 6, 2, 5, 10, 21, and 13 SNPs were identified as significantly correlated (P<8.33e-8) with BH, BL, CD, CW, CG, RW, RH, and CC, respectively. Interestingly, the GWAS for BH and RH identified the greatest number of significant SNPs, with a large proportion of these SNPs overlapping and being mainly located in four regions: chr13_63286230-69784740 (10 SNPs), chr14_60354209-60376549 (six SNPs), and chr15_65605417-73873841 (five SNPs), and chr23_42819635-43332716 (nine SNPs). Individuals carrying more of these SNPs had higher BH and RH values. After annotation for all the significant SNPs, 103 genes located in a ±100 Kb region were identified, where the most significantly enriched KEGG pathway was \"Olfactory transduction\", and the most significantly enriched GO terms were \"cellular process\" and \"molecular transducer activity\".</p><p><strong>Conclusion: </strong>This study investigated the population genetics of two prominent Chinese goat breeds and identified several SNPs that are significantly associated with body conformation traits. These findings provide biological insights on enhancing growth performance and have great potential for practical application in the genomic selection of meat goats.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of a calcium soap and jackfruit leaf extract on blood metabolites, biomarkers of oxidative stress and follicle growth of crossbreed doelings.","authors":"Yusti Pujiawati, I Komang Gede Wiryawan, Lilis Khotijah, Muhammad Agus Setiadi, Simon Petrus Ginting","doi":"10.5713/ab.25.0337","DOIUrl":"https://doi.org/10.5713/ab.25.0337","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluates the effect of a flushing ration containing polyunsaturated fatty acids (PUFA) and jackfruit leaf extracts (JLE) on the follicle growth, reproductive hormone profiles and oxidative stress state of doelings.</p><p><strong>Method: </strong>Fifteen primiparous Boer x Jawarandu doelings (23.21±3.57, 10-11 months old) were divided into three groups: control (PUFA flushing ration), control+JLE (200 mg/kg DMI), and control+Ca-soap (calcium soap)+JLE (200 mg/kg DMI). The researchers measured the following parameters production performances, blood metabolites, antioxidant status, estradiol, progesterone, and follicle development.</p><p><strong>Results: </strong>The doelings' glucose levels showed significant differences (p<0.05) during the post-flushing (PF) phase, on days 19, and day 21 after estrus. An increase in glucose level was observed following the administration of the control+Ca-soap+JLE ration. However, on day 21 after estrus, the highest glucose level was detected in the control+JLE treatment group (p<0.05). In addition, the doelings' cholesterol levels were elevated on days 19 and 21 with the administration of the control+JLE ration. These changes in glucose and cholesterol levels between phases are presumed to be associated with the increased demand for hormone synthesis and follicular development. JLE supplementation reduced malondialdehyde (MDA) levels across all phases (p<0.05), indicating lower oxidative stress. Moreover the administration of the control+JLE flushing ration resulted in the highest estradiol hormone level on day 19 after estrus compared to other treatments.This finding is consistent with the greater number of medium-sized follicles observed in doelings receiving the same treatment. Additionally,including JLE in the flushing ration also increased the doelings' progesterone hormone levels during the luteal phase.</p><p><strong>Conclusion: </strong>Jackfruit leaf extract can mitigate oxidative stress by reducing blood MDA levels during the pre-mating phase. The combination of a polyunsaturated fatty acid flushing ration with JLE may enhance follicular development, particularly medium and large-sized follicles.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of fermented rice bran extract with multi-microbial species on intestinal health and growth of nursery pigs.","authors":"Lan Zheng, Jeonghyeon Son, Sung Woo Kim","doi":"10.5713/ab.25.0402","DOIUrl":"https://doi.org/10.5713/ab.25.0402","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to investigate the effects of fermented rice bran extract (FRB) with multi-microbial species on intestinal health and growth performance of nursery pigs.</p><p><strong>Methods: </strong>Thirty weaned pigs (initial body weight = 6.8±0.8 kg) were allocated to 3 dietary treatments in a randomized complete block design (n = 10 per treatment) and fed for 25 d in 2 phases (7 and 18 d, respectively). Pigs were housed individually in pens equipped with a feeder and a nipple drinker. Pigs were fed a basal diet supplemented with 0%, 0.5%, or 1.0% FRB. The main feedstuffs of the basal diet were corn, soybean meal, whey permeate, and blood plasma. The FRB was prepared by fermenting rice bran with Lactobacillus plantarum, Bacillus subtilis, and Saccharomyces cerevisiae, and contained at least 1×107 CFU/g. On d 25, pigs were euthanized to collect intestinal tissues and mucosa for intestinal health.</p><p><strong>Results: </strong>The supplementation of FRB decreased (p<0.05) the abundance of Desulfovibrio piger in the jejunal mucosa. Malondialdehyde and protein carbonyl in the duodenum linearly decreased (p<0.05) and the villus height to crypt depth ratio in the jejunum linearly increased (p<0.05) with increasing FRB supplementation. The apparent ileal digestibility of gross energy and crude protein tended to linearly increase (p=0.084 and p=0.098, respectively). Body weight on d 25 and overall average daily gain tended to show quadratic responses (p=0.084 and p=0.095, respectively) with increasing FRB supplementation. The gain to feed ratio (G:F) was maximized when the FRB intake was 2.7 g/d (0.48%) during d 7 to 25.</p><p><strong>Conclusion: </strong>Dietary supplementation with FRB with multi-microbial species improved intestinal health based on immune response, oxidative stress, and morphology. The growth performance of nursery pigs showed quadratic responses with increasing FRB supplementation. Specifically, the G:F was maximized with supplementation of FRB at 0.48%.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mechanism of m6A methylation analysis of the transcriptome to regulate the diameter of Alpine Merino wool fiber.","authors":"Lin Yue, Ting Ting Guo, Bo Wen Chen, Jian Bin Liu, Zeng Kui Lu, Chao Yuan","doi":"10.5713/ab.25.0347","DOIUrl":"https://doi.org/10.5713/ab.25.0347","url":null,"abstract":"<p><strong>Objective: </strong>Wool is an important textile raw material, and fiber diameter is a major determinant of the economic value and quality of wool products. Analyses of the regulatory mechanisms underlying wool fiber diameter are necessary for the development of strategies to improve wool fineness. Therefore, we used methylationomics to analyze the skin tissue of individuals with different fiber diameters, and analyzed the apparent regulation mechanism of wool fiber diameter.</p><p><strong>Methods: </strong>In this study, we jointly analyzed the transcriptome and m6A methylome of skin tissues from individual Alpine Merino sheep with different wool fiber diameters (classified into three groups) to mine key methylated RNAs and explore the significance of m6A methylation in the regulation of this trait.</p><p><strong>Result: </strong>In total, 54,057 methylated peaks, 4,273 differentially methylated genes, 139 differentially methylated lncRNAs, and 2,992 differentially methylated circRNAs were found in the three comparisons. These loci were enriched in the Wnt, Notch, and TGF-β signaling pathways, as determined through GO and KEGG pathway analyses. RNA correlation analyses revealed key RNAs, such as CACNA1E, FOS, CAMK2B, RNF43, circ-0317, circ-4794, TCONS-00020832, and TCONS-00020845, indicating that hypermethylation may be an important factor affecting wool fiber diameter.</p><p><strong>Conclusion: </strong>These findings provide insight into the molecular regulatory mechanism underlying wool fiber diameter and provide a theoretical basis for the development of the wool industry.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}