Animal Bioscience最新文献

{"title":"Effects of feed supplementation with fruit peel pellet microencapsulated phytonutrients on rumen fermentation efficiency, in vitro degradability, methane production and microbial diversity.","authors":"Sukruthai Sommai, Chanon Suntara, Maharach Matra, Srisan Phupaboon, Gamonmas Dagaew, Chaichana Suriyapha, Rittikeard Prachumchai, Metha Wanapat","doi":"10.5713/ab.24.0660","DOIUrl":"https://doi.org/10.5713/ab.24.0660","url":null,"abstract":"<p><strong>Objective: </strong>The current study aimed to determine the impact of supplementing fruit peel pellet microencapsulated phytonutrients (mMARABAC) on rumen fermentation efficiency, in vitro degradability, methane production, and microbial diversity.</p><p><strong>Methods: </strong>The experiment was completely random, and the dietary treatments were mMARABAC supplements at 0, 5, 10, and 15 mg/500 mg DM of substrate (rice straw and concentrate).</p><p><strong>Results: </strong>The experiment's results demonstrated that mMARABAC supplementation significantly affected the gas production from the insoluble fraction (b), rate of gas production value (c), and potential extent of gas (|a| + b) value (p<0.05), while the soluble fraction of gas production (a) was not influenced by the treatments. Furthermore, mMARABAC increased the cumulative gas at 96 h after incubation (p<0.05) when mMARABAC was supplemented with 5 mg of total DM substrate. However, mMARABAC supplementation did not have an effect on IVDMD (p>0.05). The supplementation of mMARABAC did not significantly affect ruminal pH and NH3-N (p>0.05). Additionally, there was linearly (p<0.05) decreased CH4 production at 24 hours of incubation. The mMARABAC supplementation to the diet did not affect the concentration of total VFAs, C2, C3, C4, or the C2:C3 ratio (p>0.05). The effect of mMARABAC supplementation on F.succinogenes, R.albus, R.flavefaciens, B. fibrisolvens and M. elsdenii was different between treatments (p<0.05), while the mMARABAC supplement had an effect on Methanobacterial (p>0.05).</p><p><strong>Conclusion: </strong>This study suggested that ruminants could use agricultural by-products as a source of phytonutrients by supplementing with mMARABAC at 5 mg/500 mg DM of substrate.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination and prediction of net energy of soybean meal fed to pregnant sows by indirect calorimetry.","authors":"Lei Xue, Can Zhang, Bo Cheng, Qian Song, Lee J Johnston, Ling Liu, Fenglai Wang, Jianjun Zang","doi":"10.5713/ab.24.0663","DOIUrl":"https://doi.org/10.5713/ab.24.0663","url":null,"abstract":"<p><strong>Objective: </strong>The study was conducted to investigate the appropriate substitution level of soybean meal (SBM) for determining its net energy (NE), and establish NE prediction equation of SBM based on the determined NE values for pregnant sows.</p><p><strong>Methods: </strong>In Exp. 1, eighteen pregnant sows (Landrace×Yorkshire; parity, 2 to 3) with an initial body weight (BW) of 221.2±2.6 kg at mid-gestation were blocked by BW and randomly assigned into 3 groups. Three groups fed with a corn-SBM basal diet and two test diets with 15% and 30% energy-supplying components replaced by SBM, respectively. In Exp. 2, six diets were formulated including a corn-SBM basal diet and five SBM diets (based on the substitution level determined of Exp. 1) with different soybean sources and processing methods. Moreover, 12 pregnant pigs (BW = 209.0±3.0 kg; parity, 3 to 4) at mid-gestation were arranged in a 6×3 Youden square design.</p><p><strong>Results: </strong>Increasing substitution levels of SBM linearly increased (p<0.05) fecal and urinary nitrogen excretion and the ratio of urinary energy to digestible energy (DE), while linearly decreased (p<0.05) the ratio of metabolizable energy (ME) to DE and tended to linearly decrease dietary ME (p=0.066) and NE (p=0.074). The coefficient of variation for the NE of SBM was lower at a 15% substitution level compared to a 30% substitution level. The nutritional compositions of SBM are influenced by the soybean sources and processing methods. As dry matter basis, NE values of SBM ranged from 11.1 to 12.7 MJ/kg and the best-fitted prediction equation for NE of SBM was: NE (MJ/kg) = -91.71+5.35×gross energy (%)-0.03×neutral detergent fiber (%; R2 = 0.96).</p><p><strong>Conclusion: </strong>A substitution level of 15% was more appropriate to determine NE of SBM. Furthermore, NE values of SBM can be predicted based on their nutritional compositions.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of active components from Piper sarmentosum on the growth, intestinal barrier function, and immunity of broiler chickens.","authors":"Luli Zhou, Guanyu Hou, Hanlin Zhou, Khaled Abouelezz, Yuxiu Ye, Dingfa Wang","doi":"10.5713/ab.24.0736","DOIUrl":"https://doi.org/10.5713/ab.24.0736","url":null,"abstract":"<p><strong>Objective: </strong>Pellitorine (PT) and vitexin-2-O-rhamnoside (VR) are two active ingredients of Piper sarmentosum. Their contents are relatively high in the ethanol extract of Piper sarmentosum (PSE). However, it remains unknown whether PT and VR are the primary components of PSE that exert beneficial effects on gut health. In this study, we aimed to confirm that these two compounds are the primary anti-inflammatory active ingredients in PSE.</p><p><strong>Methods: </strong>Total of 300 female 1-day-old Danzhou chickens were randomly divided into five groups with five replicates each. Chickens were given a basal diet (CON group), a basal diet with added PSE (200 mg/kg), VR (1.321 mg/kg), PT (0.563 mg/kg), and a combination of VR + PT (1.321 mg/kg VR and 0.563 mg/kg PT) until they were 35 days of aged.</p><p><strong>Results: </strong>The findings reveal that the VR+PT group exhibited increased liver, thymus and spleen indices compared to the CON group, as well as elevated mRNA levels of ZO-1 and Claudin-1 (P < 0.05). In contrast, the VR+PT group exhibited reduced serum levels and the ileum mucosa mRNA expression levels of IL-1β and IL-6 compared to the CON group (P < 0.05). Additionally, the chickens in the VR+PT group had a greater final weight and average daily gain compared to the CON and PSE groups, with a significantly lower level of D-lactic acid in serum (P < 0.05). The serum IgM level increased significantly (P < 0.05) in the VR+PT group compared to the PSE group, while the kidney epinephrine level decreased significantly (P < 0.05).</p><p><strong>Conclusion: </strong>The present study provides preliminary confirmation that VR and PT are two of the main active ingredients in PSE, which can cooperatively improve growth performance, as well as intestinal barrier and immune function in chickens.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative and comparative analysis of whole-transcriptome sequencing in circCOL1A1-knockdown and circCOL1A1-overexpressing goat hair follicle stem cells.","authors":"Jian Wang, Xi Wu, Liuming Zhang, Xiaomei Sun, Wei Sun, Kunzhe Dong, Yongjun Li","doi":"10.5713/ab.24.0816","DOIUrl":"https://doi.org/10.5713/ab.24.0816","url":null,"abstract":"<p><strong>Objective: </strong>Hair morphogenesis is tightly related to hair follicle stem cells (HFSCs) proliferation and hair follicle (HF) development. Yangtze River Delta white goats (YRDWG) HFSCs are important for producing superior-quality brush hair (SQBH). Nonetheless, the known regulatory mechanisms are not sufficient to explain YRDWG gHFSCs growth, HF development, and SQBH formation.</p><p><strong>Methods: </strong>To deeply investigate the interaction networks and mechanisms of circCOL1A1 in the HF development and SQBH formation of YRDWG in detail, we applied whole-transcriptome sequencing and bioinformatics analysis of circCOL1A1-knockdown and circCOL1A1-overexpressing HFSCs from YRDWG. STRING and other databases were used to construct multiple interaction networks. Differentially expressed (DE) genes, DE-miRNAs, and DE-circRNAs were further confirmed via real-time quantitative polymerase chain reaction (RT-qPCR) and Sanger sequencing.</p><p><strong>Results: </strong>A total of 87 genes, 96 miRNAs, and 135 circRNAs were differentially expressed between circCOL1A1-knockdown and circCOL1A1-overexpressing gHFSCs. Functional enrichment, GO annotation and KEGG analyses identified marked enrichment of these DE-genes, DE-miRNAs, and DE-circRNAs in the MAPK, PI3K/Akt, and focal adhesion signaling pathways, which are closely associated with gHFSCs growth and HF development. In addition, through interaction network construction, four important regulatory axes were obtained, namely, the chi-circCOL1A1-miR-149-5p-CMTM3-AR, chi-circACTN1-miR-671-5p-MAPK3/COL13A1, chi-circITGA6-miR-18a-5p-FGF1/MAP3K1 and chi-circCOBLL1-miR-30a-5p/miR-128-3p-ITGA6/MAPK14/FGF14 axes.</p><p><strong>Conclusion: </strong>These novel findings provide a valuable and comprehensive basis for investigating the complex mechanism by which circRNAs participate in and regulate HF development and SQBH formation in Yangtze River Delta white goats.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effect of corn straw and mulberry leaves fermantation in vitro and effects on growth performance and antioxidant capacity of sheep.","authors":"Qirui Hou, Wenfeng Zhang, Wenyu Hou, Weiguo Zhao, Hui Xie, Peng Zhao, Shuli Wang, Yongen Zhang","doi":"10.5713/ab.24.0763","DOIUrl":"https://doi.org/10.5713/ab.24.0763","url":null,"abstract":"<p><p>This study was to determine the synergistic effect of corn straw and mulberry leaves in sheep diet. Corn straw and mulberry leaves were mixed as culture substrate for six groups: 100:0 (T0), 80:20 (T20), 60:40 (T40), 40: 60 (T60), 20:80 (T80) and 0:100 (T100). The in vitro culture indicated that mulberry leaves exhibited a positive correlation with theoretical gas production (GP), cumulative GP, microbial crude protein concentration, and organic matter digestibility. At 72 h, total volatile fatty acids and acetic acid were significantly higher in the T60, T80, and T100 groups (P < 0.05). Moreover, at 6 h, 12 h, and 72 h, the ammonia nitrogen of T60, T80, and T100 groups was significantly higher (P < 0.05). The combination of T20, T60, and T80 were selected for sheep feeding, and T60 got an highest increase in the average daily feed intake (P < 0.05). In addition, the thickness of the rib and back was notably increased in the T80 (P < 0.05). The water holding capacity of the longissiums dorsi muscle was higher and drip loss lower by feeding combination feed, especially in T20 and T60 groups (P < 0.05). The blood urea nitrogen decreased with the increase of mulberry leaf content (P < 0.05). Additionally, the T60 and T80 groups demonstrated higher DPPH and ABTS free radical scavenging ability (P < 0.05). Furthermore the combination resulted in a notable enhancement of catalase and glutathione peroxidase activity in the sheep liver (P < 0.05). In conclusion, the combination of corn straw and mulberry leaves had a positive synergistic effect in improving feed intake, muscle thickness, muscle water retention and liver antioxidant capacity of sheep, which provides a practical way for utilizing agricultural waste.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary rumen undegradable protein:rumen degradable protein ratio on nitrogen metabolism in Hanwoo steers.","authors":"Sang Yeob Kim, Rajaraman Bharanidharan, Seyun Im, Kyoung Hoon Kim, Joonpyo Oh, Hyun Jin Kim, Jaesung Lee, Kamburawala Kankanamge Tharindu Namal Ranaweera, Jin Woo Jeong, Jun Seok Oh, Sang Hyun Lee, Myunggi Baik","doi":"10.5713/ab.24.0592","DOIUrl":"https://doi.org/10.5713/ab.24.0592","url":null,"abstract":"<p><strong>Objective: </strong>We investigated the effects of dietary rumen undegradable protein (RUP):rumen degradable protein (RDP) ratio on growth performance, nitrogen (N) metabolism, and rumen and blood parameters in Hanwoo (Korean cattle) steers.</p><p><strong>Methods: </strong>Eight Hanwoo steers (average body weight, 393 kg) were allocated to two groups and fed with a high RUP:RDP ratio (46.9:53.1 based on crude protein) or a low RUP:RDP ratio (30.6:69.4) concentrate with iso-crude protein content in a 2×2 Latin square design in two successive periods.</p><p><strong>Results: </strong>The high RUP:RDP group had higher (p<0.01) average daily gain, and lower (p<0.05) ruminal ammonia and plasma urea N concentrations than the low RUP:RDP group. The high RUP:RDP group had lower (p<0.05) urinary N excretion and urinary N per N intake and higher (p<0.1) tendency of retained N than the low RUP:RDP group. The high RUP:RDP group had higher (p<0.1) tendency of N utilization efficiency (retained N per N intake: 28.7% vs. 25.5%) than the low RUP:RDP group. The high RUP:RDP group had a lower (p<0.1) tendency of urinary N per total N excretion, but a higher tendency of fecal N per total N excretion.</p><p><strong>Conclusion: </strong>A high ratio of dietary RUP:RDP may improve N utilization efficiency by reducing urinary N excretion, which may be beneficial for the environment through reducing atmospheric ammonia emissions.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Effects of Processed Soybean Meal on Intestinal Health and Growth of Nursery Pigs with Pharmacological Level of Zinc.","authors":"Lan Zheng, Jung Yeol Sung, Sung Woo Kim","doi":"10.5713/ab.24.0767","DOIUrl":"https://doi.org/10.5713/ab.24.0767","url":null,"abstract":"<p><strong>Objective: </strong>The objective was to evaluate the effects of partially replacing soybean meal (SBM) in nursery pig diets with enzyme-treated soybean meal (ESBM), fermented soybean meal (FSBM), or fermented soybean meal containing probiotic microorganism (PFSBM) on jejunal mucosa-associated microbiota, immune responses, intestinal morphology, and growth performance of nursery pigs.</p><p><strong>Methods: </strong>Forty-eight weaned pigs (initial body weight = 7.8 ± 0.7 kg) were randomly allocated to four dietary treatments in a randomized complete block design and fed for 25 d in three phases (5, 10, and 12 d, respectively). Dietary treatments were corn-SBM-based diet (SBM diet) and the SBM diet in which 70 g/kg of SBM was replaced with ESBM (ESBM diet), FSBM (FSBM diet), or PFSBM (PFSBM diet). Zinc oxide was supplemented at 2.5 g/kg (2,000 mg/kg of zinc) in the experimental diets for phases 1 and 2. Pigs were housed individually in pens (1.50 m × 0.74 m) equipped with a feeder and a nipple drinker. Serum was collected on d 24 and pigs were euthanized on d 27 to collect tissues and mucosa in the jejunum. The PFSBM diet increased (p<0.05) the relative abundance of Lactobacillus, whereas it tended to decrease (p=0.072) the abundance of Pseudomonas compared with the SBM diet. The ESBM diet increased (p<0.05) gain to feed ratio from d 5 to 15 compared with the SBM diet. However, partially replacing SBM at 70 g/kg with ESBM, FSBM, or PFSBM did not affect immune responses in serum and jejunal mucosa, intestinal morphology in the jejunum, and overall growth performance of nursery pigs.</p><p><strong>Conclusion: </strong>Partially replacing soybean meal with various processed soybean meal did not affect immune responses, intestinal morphology, and overall growth performance when diets were supplemented with zinc at pharmacological level in early phases.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-21-5p promotes differentiation and reduces oleic acid-induced lipid droplet accumulation in C2C12 myoblasts by targeting FBXO11.","authors":"Yi Li, Jiayuan Fang, Yingying Jiao, Qinchuan Lv, Xingyu Xiao, Shuo Zheng, Xi Chen, Jie Song, Xunnming Zhang, Libo Zhang, Ze Ma, Changhong Li, Linlin Hao","doi":"10.5713/ab.24.0665","DOIUrl":"https://doi.org/10.5713/ab.24.0665","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the function of miRNA 21-5p in regulating the differentiation of C2C12 myoblasts and intramuscular lipid droplet accumulation in myotubes.</p><p><strong>Methods: </strong>The role of miR-21-5p in the proliferation and differentiation of myofibroblasts and intracellular lipid accumulation was analyzed using bioinformatics, CCK-8 assay, RT-qPCR, immunoblotting, immunofluorescence staining, and Oil Red O staining.</p><p><strong>Results: </strong>Analysis of porcine BodyMap transcriptomic data revealed differential expression of miRNA 21-5p in skeletal muscle and adipose tissue. Bioinformatics analysis combined with a dual-luciferase reporter assay demonstrated that FBXO11 serves as a direct target of miR-21-5p. Transfection experiments involving a miR-21-5p mimic, miR-21-5p inhibitor, and si-FBXO11 in C2C12 cells showed that overexpression of miR-21-5p or silencing of FBXO11 significantly enhanced the proliferation of C2C12 cells, upregulated myogenesis-related factors, and promoted myotube formation. Additionally, oleic acid-induced lipid accumulation in myotubes was suppressed, accompanied by reduced expression of adipogenesis-related genes. Conversely, inhibition of miR-21-5p expression produced opposite effects.</p><p><strong>Conclusion: </strong>These findings indicate that miR-21-5p promotes proliferation and differentiation while inhibiting intramyocellular lipid deposition by targeting the 3'-untranslated region (3'UTR) of FBXO11 in myogenic cell. The results suggest that miR-21-5p could serve as a potential miRNA biomarker for regulating intramuscular adipogenesis.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of DNA methylation changes following Cfp1 knockout in mouse spermatocytes.","authors":"Chanhyeok Park, Youngsok Choi, Seonho Yoo, Hyeonwoo La, Kwonho Hong","doi":"10.5713/ab.24.0807","DOIUrl":"https://doi.org/10.5713/ab.24.0807","url":null,"abstract":"<p><strong>Objective: </strong>Spermatogenesis is a complex biological process that encompasses meiosis in spermatocytes and the dynamic epigenetic alterations that ensure the inheritance of genetic traits. CXXC finger protein 1 (CFP1, Cfp1, CXXC1, Cxxc1) is a critical component of the SET domain-containing 1A histone lysine methyltransferase complex that catalyzes histone H3K4 methylation and has a specific binding domain for unmethylated CpG DNA. However, our current understanding of CFP1's role in the genome-wide regulation of DNA and H3K4 methylation remains limited.</p><p><strong>Methods: </strong>We performed genome-wide methylation analysis using reduced-representation bisulfite sequencing (RRBS) on spermatocytes isolated from Cfp1 knockout (KO) and wild-type (WT) mice. Promoter methylation changes were integrated with publicly available microarray and ChIP-seq data to identify genes regulated by CFP1.</p><p><strong>Results: </strong>CFP1 depletion led to significant alterations in DNA methylation, particularly in promoter regions of genes associated with meiosis, transcription regulation, and chromatin remodeling. A total of 21 genes were identified as direct targets of CFP1, exhibiting reduced promoter methylation and CFP1 binding.</p><p><strong>Conclusion: </strong>Our study findings contribute to elucidating the regulatory mechanisms of CFP1 in spermatocytes, providing valuable insights into the reproductive process and advancing our understanding of the underlying causes of infertility.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of High-Protein Concentrates with Different Rumen Undegradable-to-Degradable Protein Ratios on Performance and Carcass Traits in 26-Month-Slaughtered Hanwoo Steers.","authors":"Woo Hyeong Hong, Rajaraman Bharanidharan, Taehoon Kim, Jun Suk Byun, Geumhwi Bang, Krishnaraj Thirugnanasambantham, Joonpyo Oh, Ridha Ibidhi, Sun Sik Jang, Kyoung Hoon Kim","doi":"10.5713/ab.24.0728","DOIUrl":"https://doi.org/10.5713/ab.24.0728","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the effects of high-protein concentrates with two different rumen undegradable protein (RUP)-to-degradable protein ratios on the performance and carcass characteristics of Hanwoo steers reared for 26 months.</p><p><strong>Methods: </strong>Thirty-two Hanwoo steers (217 ± 13 kg) were randomly allocated to eight pens (four animals/pen) and assigned to one of two concentrate treatments (four pens/treatment), low-RUP concentrate (LRUP; RUP ratio of 37:63) or high-RUP concentrate (HRUP; RUP ratio of 47:53), with a dietary crude protein (CP) content of 20% dry matter (DM) during the growing stage (8-17 months) and 18% DM during the fattening stage (18-26 months).</p><p><strong>Results: </strong>Increasing the RUP ratio in the concentrate reduced (p = 0.062) rumen ammonia (NH3-N) levels during the growing stage. A decrease (p < 0.05) in the molar proportions of acetate and valerate, and an increase (p = 0.097) in the proportions of iso-valerate, was observed in the HRUP group. Significant improvements (p < 0.05) in the average daily gain and the feed conversion ratio were observed in the HRUP group during the fattening stage. Although there were no significant group differences in carcass yield or characteristics in steers slaughtered at 26 months, a trend (p = 0.081) toward an increased rib-eye area was observed in the HRUP group. Relative mRNA expression profiling revealed higher lipid biosynthesis and lipolysis in the HRUP group at slaughter. However, no effects on intramuscular fat content or fatty acid profiles were detected.</p><p><strong>Conclusion: </strong>Raising the RUP level improved performance and intramuscular fat metabolism in Hanwoo steers. The final body weight of the steers in the HRUP group was comparable to that of Hanwoo steers raised under the conventional 30-month fattening period. Overall, feeding a high-CP concentrate with a higher RUP proportion (47% CP) may be a beneficial strategy to shorten the feeding period.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}