BMP8A/TGF-β1 regulates chicken chondrocyte proliferation, differentiation, and apoptosis induced by Thiram.

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Yuxiang Lu, Hengyong Xu, Xuyang Ji, Yuxin Zhou, Zhi Hu, Felix Kwame Amevor, Ranran Du, Xiaoling Zhao, Yiping Liu, Yan Wang
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引用次数: 0

Abstract

Objective: Tibial dyschondroplasia (TD) is a metabolic cartilage disorder that impairs the development of the tibial growth plate in rapidly growing poultry. This study aimed to identify key genes and clarify the molecular mechanisms involved in tibial dyschondroplasia (TD) in broiler chickens. It also evaluated the potential effect of vitamin D3 (VD3) in alleviating TD symptoms, focusing particularly on the role of BMP8A and its interaction with transforming growth factor-β1 (TGF-β1).

Methods: 94 broiler chicks were divided into three groups: healthy control, thiram-induced TD, and thiram-induced with VD3 supplementation. RNA sequencing was performed to identify differentially expressed genes (DEGs) across groups. Target genes were further validated using molecular biology techniques, including gene expression analysis and in vitro functional assays on chondrocytes.

Results: VD3 effectively mitigated thiram-induced chondrocyte damage. RNA-seq revealed 625 DEGs enriched in pathways such as the TGF-β signaling pathway. Four co-differentially expressed genes (BMP8A, COL10A1, SDC3, and SCIN) were closely associated with collagen metabolism and reorganization. Functional assays, such as CCK8, EdU and IHC showed that BMP8A mitigated collagen accumulation induced by elevated TGF-β1 levels, promoted the release of collagen types I, II, and X, and facilitated chondrocyte proliferation and differentiation while reducing apoptosis.

Conclusion: BMP8A plays a protective role in TD by regulating collagen balance and maintaining chondrocyte function, especially under high TGF-β1 conditions. VD3 supplementation effectively reduces TD-related damage. The interaction between BMP8A and TGF-β1 may provide a novel therapeutic target for preventing and treating TD in poultry.

BMP8A/TGF-β1调控Thiram诱导的鸡软骨细胞增殖、分化和凋亡。
目的:胫骨软骨发育不良(TD)是一种影响快速生长家禽胫骨生长板发育的代谢性软骨疾病。本研究旨在鉴定肉仔鸡胫骨软骨发育不良(TD)的关键基因并阐明其分子机制。该研究还评估了维生素D3 (VD3)在缓解TD症状方面的潜在作用,特别关注了BMP8A的作用及其与转化生长因子-β1 (TGF-β1)的相互作用。方法:将94只肉仔鸡分为健康对照组、硫胺诱导TD组和添加VD3诱导的硫胺组。进行RNA测序以鉴定各组间的差异表达基因(DEGs)。利用分子生物学技术进一步验证靶基因,包括基因表达分析和软骨细胞的体外功能测定。结果:VD3能有效减轻thiram诱导的软骨细胞损伤。RNA-seq显示625个基因在TGF-β信号通路等通路中富集。四个共差异表达基因(BMP8A、COL10A1、ssd3和SCIN)与胶原代谢和重组密切相关。CCK8、EdU、IHC等功能检测显示,BMP8A可减轻TGF-β1水平升高引起的胶原积累,促进I、II、X型胶原的释放,促进软骨细胞增殖分化,减少细胞凋亡。结论:BMP8A通过调节胶原平衡,维持软骨细胞功能在TD中发挥保护作用,特别是在TGF-β1高水平的情况下。补充VD3可有效减少td相关损伤。BMP8A与TGF-β1的相互作用可能为防治禽TD提供新的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
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