谷氨酸和谷氨酰胺通过能量代谢促进雏鸡肠道发育的比较研究。

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Peiyu Huang, Yaoming Cui, Wenjing Liang, Li Zhang, Junquan Tian, Liping Gan, Linna Guo, Weiyu Chen, Guohao Yang, Junjun Guan
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引用次数: 0

摘要

目的:研究谷氨酸(Glu)和谷氨酰胺(Gln)对脂多糖(LPS)损伤蛋鸡肠道发育的影响。方法:选取健康0日龄海兰褐鸡240只,随机分为4个处理,每个处理6个重复。在8日龄和11日龄时,除对照组外,所有雏鸟均接受两次LPS治疗。将lps攻击的雏鸟分为3个饲粮处理组:基础饲粮(不含添加剂)、0.05%谷氨酰胺补充饲粮和0.20%谷氨酰胺补充饲粮。结果:LPS诱导蛋鸡肠道损伤,抑制肠道发育,表现为生长性能显著降低、肠道参数变差、肠道形态变差(p)。结论:饲粮中添加0.05% Glu和0.20% Gln可通过促进上皮细胞增殖和分化,改善蛋鸡生长性能,促进肠道发育,修复肠道损伤。此外,0.20%谷氨酸比0.05%谷氨酸表现更好,这可能是由于能量代谢更优。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative study on the effects of glutamic acid and glutamine in promoting intestinal development in chicks through energy metabolism.

Objective: This study evaluated the effects of glutamic acid (Glu) and glutamine (Gln) on the intestinal development of layer chicks with lipopolysaccharide (LPS)-induced damage.

Methods: A total of 240 healthy 0-d-old Hy-Line Brown chicks were randomly assigned to 4 treatments, each with 6 replicates. At 8 and 11 d of age, all birds (except for the control group) received two administrations of LPS. The LPS-challenged birds were divided into three dietary treatment groups: a basal diet (without additives), a 0.05% Glu-supplemented diet, and a 0.20% Gln-supplemented diet.

Results: The LPS challenge induced intestinal injury and suppressed intestinal development in layer chicks, as evidenced by significantly reduced growth performance, poor intestinal parameters, and morphology (p<0.05). Compared to the LPS group, dietary supplementation with 0.05% Glu and 0.20% Gln significantly enhanced average daily gain (ADG), average daily feed intake (ADFI), body weight (BW), and intestinal development parameters (including length, weight, villus height, villus height/crypt depth) of duodenum, jejunum and ileum (p<0.05). These results could be attributed to upregulated mRNA expression levels of Mucin-2, E-cadherin, Dclk-1, Vil-1, Lysozyme, ChgA, Lgr-5, Bmi-1, ATP5F1AZ, and β-catenin (p<0.05). Furthermore, dietary supplementation with 0.20% Gln outperformed 0.05% Glu in enhancing BW, ADG, and ileum parameters (weight, length, epithelial cell count, and energy metabolism) (p<0.05). Additionally, intestinal organoids supplemented with 10 μM Gln had significantly higher mean area, E-cadherin gene expression, and ATP content compared with those treated with 5 μM Glu in vitro (p<0.05).

Conclusion: Dietary supplementation with 0.05% Glu and 0.20% Gln could improve growth performance, intestinal development, and repair intestinal damage in layer chicks through enhanced epithelial proliferation and differentiation. Moreover, 0.20% Gln performed better than 0.05% Glu, which may be attributed to superior energy metabolism.

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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
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