精制猪粪液体肥料中褐小球藻的安全性评价。

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Seukchan Kim, Soo-Ryang Kim, Jungho Moon, Ji-Won Jung, Sungha Hong, Sun-Goo Hwang, Myung-Gyu Lee, Meejung Ahn
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引用次数: 0

摘要

摘要目的:fusca小球藻是一种在可持续生物技术中具有广阔应用前景的微藻,因其营养价值、环境效益和生物活性而受到越来越多的关注。研究了在猪粪精制液肥中培养镰刀菌的安全性和可行性。方法:采用猪粪精制液肥作为培养褐霉的营养培养基。为了比较其与商业小球藻产品的生化特性,对培养的小球藻进行了近似成分分析,以确定其水、粗蛋白质、粗脂肪和无灰分提取物的含量。通过MTT和WST-1测定细胞毒性,并通过体重变化和血清生物标志物对Sprague-Dawley大鼠进行急性毒性研究,评估生理效应。对肝脏、肾脏和肺部进行组织病理学检查,以检测是否有毒理学影响。此外,还对抗生素残留、微生物安全性、农药污染和重金属含量进行了评估,以确认产品的总体安全性。结果:近似分析显示,与商业小球藻产品相比,其水分和粗脂肪含量存在显著差异。细胞毒性试验表明,浓度越高,免疫细胞活性越强(P < 0.05)。大鼠体重未见明显变化,血清碱性磷酸酶水平在1000和2000 mg/mL处理组呈剂量依赖性降低(P < 0.05和P < 0.01)。组织病理学检查证实肝、肾和肺无明显改变,支持其作为膳食补充剂的安全性。抗生素残留、微生物安全性、农药污染和重金属分析显示,其水平无法检测到或低于安全限度,证实了fusca的安全性。结论:用猪粪精制液肥培养褐小球藻是安全的,可用于多种生物技术应用。我们的发现暗示了它作为一种生物资源的潜力,包括作为饲料添加剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of the Safety of Chlorella fusca Grown in Refined Swine Manure Liquid Fertilizer for Bioresource Applications.

Objective: Chlorella fusca, a microalga with promising applications in sustainable biotechnology, is of growing interest for its nutritional value, environmental benefits, and bioactive properties. This study investigated the safety and viability of cultivating C. fusca in refined liquid fertilizer derived from pig manure.

Methods: Refined liquid fertilizer derived from swine manure was used as a nutrient medium for cultivating C. fusca. To compare its biochemical properties with those of commercial Chlorella products, the cultivated algae were subjected to proximate composition analyses to determine their water, crude-protein, crude-fat, and ash-free-extract contents. Cytotoxicity was assessed via MTT and WST-1 assays, and an acute toxicity study was performed in Sprague-Dawley rats to evaluate physiological effects according to body weight changes and serum biomarkers. Histopathological examination of the liver, kidneys, and lungs was conducted to detect any toxicological effects. Additionally, antibiotic residues, microbial safety, pesticide contamination, and heavy-metal content were evaluated to confirm overall product safety.

Results: Proximate analysis revealed significant differences in moisture and crude-fat content compared to commercial Chlorella products. Cytotoxicity assays demonstrated enhanced immune cell activity at higher concentrations (P < 0.05). No significant body weight changes were detected in rats, and serum analysis indicated a dose-dependent reduction in alkaline phosphatase levels in 1000- and 2000-mg/mL treatment groups (P < 0.05 and P < 0.01, respectively). Histopathological examination confirmed the absence of significant alterations in the liver, kidneys, and lungs, supporting its safety as a dietary supplement. Antibiotic residue, microbial safety, pesticide contamination, and heavy-metal analyses showed levels that were undetectable or below safety limits, confirming the safety of C. fusca.

Conclusions: Chlorella fusca cultivated with refined liquid fertilizer derived from swine manure was found to be safe for use in various biotechnology applications. Our findings imply its potential as a bioresource, including as a feed additive.

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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
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