Animal Bioscience最新文献

{"title":"Inbreeding depression of reproductive traits in Japanese Black cattle using genomic information.","authors":"Takayuki Ibi, Souma Kouno","doi":"10.5713/ab.25.0113","DOIUrl":"https://doi.org/10.5713/ab.25.0113","url":null,"abstract":"<p><strong>Objectives: </strong>The objective of this study was to evaluate genomic inbreeding in Japanese Black cattle and its effects on reproductive traits.</p><p><strong>Methods: </strong>The study analyzed reproductive records and SNP data from Japanese Black cattle born between 2001 and 2005, resulting in 8,553 records from large farms. Genomic inbreeding was assessed using SNP data from 782 animals. Key reproductive traits analyzed included age at first calving (AFC) and calf production index (CPI). Statistical models were employed to estimate fixed effects and inbreeding depression.</p><p><strong>Results: </strong>Findings indicated that genomic inbreeding levels were generally higher than traditional inbreeding coefficients. Significant inbreeding depression was observed in CPI but not in AFC. Specific chromosomes (12, 15, 27 for CPI and 4 for AFC) showed significant associations with inbreeding depression. Key genes related to reproduction were identified, particularly on chromosome 15.</p><p><strong>Conclusion: </strong>The study concludes that genomic inbreeding effectively detects inbreeding depression related to reproductive abilities in Japanese Black cattle. The results highlight the importance of genomic assessment in breeding practices to enhance reproductive performance while maintaining genetic diversity in the breed.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bridging farmers' perceptions and laboratory science: wheat bran quality assessment in smallholder dairy systems: technical note.","authors":"Alemayehu Tadesse, Yayneshet Tesfay, Veerle Fievez","doi":"10.5713/ab.24.0810","DOIUrl":"10.5713/ab.24.0810","url":null,"abstract":"<p><p>This study was conducted to evaluate wheat bran (WB) quality characteristics based on particle size distribution and to assess how farmers' rankings align with laboratory results. Thirty smallholder dairy farmers qualitatively scored five WB types from five major wheat flour processing factories in Tigray, Ethiopia, on a scale of 1 to 4 for water holding capacity (WHC) and nutritive value (1 = poor to 4 = excellent), as well as the risk of digestive disorders (1 = high to 4 = very low). Laboratory analyses included physical parameters, chemical analysis, and in vitro digestibility. The geometric mean particle size of WB ranged between 908 to 1,103 μm, and the WHC between 2.15 to 2.90 mL/g. Farmers' scores for nutritive value correlated positively with crude protein (rspearman's = 0.347; p<0.05) and effective rumen degradability of crude protein (rspearman's = 0.291; p<0.05), and negatively with particle size (rspearman's = -0.553; p<0.05). Scores for WHC positively correlated with particle size (rspearman's = 0.526; p<0.05). The present findings revealed that particle size distribution is the predominant qualitative selection criteria for farmers (e.g., on the market) to assess WB quality, and this qualitative appreciation is to some extent related to chemical characteristics and rumen degradability.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1915-1920"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New prediction models for gross energy of pig urine using urinary nitrogen concentration and body weight: technical note.","authors":"Hyunseok Do, Bokyung Hong, Jeonghyeon Son, Noa Park, Beob Gyun Kim","doi":"10.5713/ab.25.0097","DOIUrl":"10.5713/ab.25.0097","url":null,"abstract":"<p><p>The objectives were to evaluate previous equations for estimating gross energy (GE) of pig urine using urinary nitrogen (N) and to develop novel equations for estimating GE concentrations of pig urine. A total of 136 urine samples were obtained from pigs fed 18 diets in 2 experiments. The samples were analyzed for GE and N concentrations. The accuracy of previous equations was assessed by regressing the measured values minus the predicted values for urinary GE concentration on the predicted values centered to the mean. Novel equations for estimating the urinary GE concentration were developed using urinary N concentration and body weight (BW) as independent variables. The previous equations overestimated (mean bias; p<0.001) urinary GE concentrations and the overestimation was more pronounced for the low-GE urine samples (linear bias; p<0.001). The novel equations developed in the present work were: urinary GE concentration (kcal/kg) = -7.51+12.83×urinary N (r2 = 0.92 and p<0.001) and -16.33+14.00×urinary N+0.192×BW-0.030×urinary N×BW (R2 = 0.92 and p<0.001) where N as g/kg and BW as kg. Overall, the previous equations overestimate urinary GE, particularly for low-GE urine of pigs. Gross energy concentrations in urine can be fairly accurately estimated using urinary N concentration and BW.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1953-1958"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyvinylpyrrolidone can effectively improve the efficiency of resiquimod in sorting bovine Y sperm.","authors":"Fei Huang, Peng Niu, Hui-Min Qu, Hong Cheng, Jie-Ru Wang, Jia-Jia Suo, Jie Wang, Di Fang, Qing-Hua Gao","doi":"10.5713/ab.24.0738","DOIUrl":"10.5713/ab.24.0738","url":null,"abstract":"<p><strong>Objective: </strong>The X/Y sperm separation technique plays a crucial role in gender control. The objective of this experiment is to investigate the effect of polyvinylpyrrolidone (PVP) concentration (A: 0%, B: 1%, C: 3%, D: 5%, E: 7%, vol/vol) on Y sperm sorting efficiency, based on the specific binding of Resiquimod (R848) to toll-like receptor (TLR)7/8 receptors on the tail of X sperm.</p><p><strong>Methods: </strong>The different concentrations of PVP were added to the R848 sperm sorting solution to facilitate the separation of Y sperm. Subsequently, the isolated sperm were subjected to quantification and motility assessment using computer-assisted semen analysis system. The X/Y sperm ratio is then analyzed by flow cytometry. The sorted sperm were evaluated for acrosomal and plasma membrane integrity. The spermatozoa were then subjected to immunofluorescent staining through immunofluorescence (IF) techniques, which preceded the quantification of the negative sperm rate. The proportion of male embryos was determined through embryonic sex identification after in vitro fertilization.</p><p><strong>Results: </strong>Flow cytometry analysis results showed that when the PVP concentrations were 3%, 5% and 7%, the proportion of Y sperm was not statistically significant, (p≥0.05). However, these percentages were significantly elevated compared to those obtained with 0% and 1% PVP concentrations (p<0.05). The IF staining results demonstrated that the proportion of TLR7/8-negative sperm remained statistically unchanged across PVP concentrations of 3%, 5%, and 7% (p≥0.05). However, these percentages were significantly elevated compared to those obtained with 0% and 1% PVP concentrations (p<0.05). The generation of male blastocysts was significantly higher at a PVP concentration of 3% compared to 0% and 1% (p<0.05), but showed no significant difference from 5% and 7% (p≥0.05).</p><p><strong>Conclusion: </strong>Selecting a 3% PVP concentration not only ensures sufficient sperm yield but also promotes effective selection of Y-sperm. These findings provide empirical evidence supporting the high-efficiency separation of X/Y sperm in livestock.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1881-1893"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Cordyceps militaris solid medium on the growth performance, immunity parameters and intestinal health of broilers.","authors":"Xiaoya Wang, Jingyan Zhang, Kang Zhang, Guowei Xu, Zhiting Guo, Xueyan Gu, Saad Ahmad, Liping Huang, Shuqi Liu, Jianxi Li, Lei Wang","doi":"10.5713/ab.24.0791","DOIUrl":"10.5713/ab.24.0791","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the impact of Cordyceps militaris solid medium (CMM) on the growth performance, immunity parameters, intestinal health and gut microbiota of broilers.</p><p><strong>Methods: </strong>A total of 320 male broilers were randomly allocated into 4 groups, consisting of 8 replicates and 10 birds per replication. The broilers were fed diets comprising the Control group, 0.02% CMM group (Low CMM), 0.2% CMM group (Mid CMM), or 2% CMM group (High CMM). Samples were collected on 14d and 28d of the experiment. Serum was collected for the determination of biochemical indices and immunoglobulins, the intestinal morphology of the duodenum, jejunum and ileum was observed, and cecum contents were collected for 16S rRNA gene sequencing.</p><p><strong>Results: </strong>Over the course of the 28d trial, the broilers fed CMM had a higher average daily gain and lower feed conversion rate than the control broilers at Days 14 and 28. Notably, compared with those of the Control group, the Low CMM diet increased the thymus and bursa indices significantly (p<0.05). Enzyme-linked immunosorbent assay revealed that CMM increased the IgA, IgM, and IgG levels in the serum, and the IgA level in the Low and Mid CMM groups was significantly higher than that in the Control group (p<0.05). A Low CMM diet showed the strongest ability to increase villus height (VH) and villus height/crypt depth, and CMM affected the relative mRNA expression levels of inflammatory factors in jejunal tissue. The 16S rRNA results showed that the CMM group presented a greater relative abundance of Firmicutes and a higher Firmicutes/Bacteroidetes ratio than did the Control group at 14 d and 28 d, and the Deferribacteres abundance also increased (14 d). At the genus level, a Low CMM diet decreased Bacteroides abundance (p<0.05) but increased the abundance of Synergistetes (p>0.05).</p><p><strong>Conclusion: </strong>These results illustrate that 0.02% CMM in the diet can promote growth performance, increase immunity, improve intestinal morphology, and regulate the intestinal flora of broilers.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1959-1972"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomics unveils chemical modifications on protein side chains in raw breast meat of broilers (Gallus gallus) affected with growth-related myopathies.","authors":"Yuwares Malila, Sawanya Charoenlappanit, Narumon Phaonakrop, Yanee Srimarut, Sittiruk Roytrakul","doi":"10.5713/ab.24.0892","DOIUrl":"10.5713/ab.24.0892","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate how growth-related myopathies influenced chemical modifications formed on amino acid residues of chicken breast proteins.</p><p><strong>Methods: </strong>Breasts (pectoralis major) of commercial broilers (Gallus gallus) were classified into \"normal\", White Striping (WS)\" and \"White Striping + Wooden Breast (WS+WB)\" groups (n = 9 per group). The meat was subjected to proteomic analysis using a liquid chromatography-tandem mass spectrometry. Differences in abundance of modified sites, including methylated lysine (Lys) and arginine, acetsylated Lys, and oxidized methionine (Met), due to the growth-related myopathies were identified (false discovery rate [FDR]<0.05). Biological functions of the proteins were analyzed.</p><p><strong>Results: </strong>Proteomics revealed 185, 105, and 194 modified sites for methylation, Lys acetylation and Met oxidation, respectively. Of 185, 10 sites from seven proteins (TPM1, MYH, MYH1F, DICER1, RCJMB04_5k17, TPI1, and VIM) showed differential abundance in the methylation (FDR<0.05). Seven acetylated Lys sites from five proteins (TPM1, ADHFE1, SPAG9, PCNT, and RCJMB04_5k17) were differentially expressed. The abundance of those sites in normal samples were lower than those of WS samples (FDR<0.05). As for oxidized Met, differential 62 sites were identified (FDR<0.05). The major Met-oxidized protein was MYH. Met oxidation of 40 sites from 22 proteins was increased in WS samples whereas 19 sites of four proteins (MYL11, MYH, MYH1F, and TNNT2) were increased in WS+WB samples. Only four sites from DICER1, LDHA and LDB3 were found in normal samples (FDR<0.05).</p><p><strong>Conclusion: </strong>The findings shed light on the links between oxidative stress and oxidized Met in the chicken with growth-related myopathies. In addition, methylation and acetylation modifications likely played a role in dynamic cell signaling to maintain cellular activities, particularly metabolism and energy production, against the stress in the affected birds.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2008-2020"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary crude protein levels on growth performance, rumen characteristics, blood metabolites, and methane emissions in finishing Hanwoo steers.","authors":"Joonpyo Oh, Hyunjin Cho, Namkyu Kang, Mingyung Lee, Md Raihanul Hoque, Yoo Yong Kim, Seongwon Seo","doi":"10.5713/ab.25.0063","DOIUrl":"10.5713/ab.25.0063","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to investigate the effects of increasing dietary crude protein (CP) concentration on growth performance, rumen characteristics, blood metabolites, and methane (CH4) emissions in finishing Hanwoo steers.</p><p><strong>Methods: </strong>Twenty-four 26-month-old Hanwoo steers (717±50.1 kg) were utilized in a 12-week study based on a completely randomized block design. The animals were randomly assigned to one of four dietary treatments by feeding concentrate mixes with different CP levels (15.0%, 16.2%, 17.5%, and 18.5% on a dry matter [DM] basis). Forage was fed ad libitum, while the concentrate mix was provided in a fixed amount.</p><p><strong>Results: </strong>Forage and total dry matter intake (DMI) linearly increased (p≤0.029) with increasing CP levels while concentrate intake was not affected by treatments. Initial and final body weight and average daily gain were not different among treatments. Feed efficiency showed a trend of linear decrease (p = 0.092) with increasing CP levels. Rumen parameters, including ruminal pH, total volatile fatty acid, molar proportions of acetate, propionate, isobutyrate, butyrate, isobutyrate, valerate, and acetate to propionate ratio, were not affected by treatments. Treatments also did not affect blood concentrations of total protein, urea, glucose, non-esterified fatty acid, albumin, creatinine, triglyceride, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, calcium, and phosphorus. Concentrations of CH4 from respiration or eructation were not affected by dietary CP. CH4 ppm per DMI and neutral detergent fiber (NDF) intake was not also different among treatment in respiration or eructation. However, CH4 ppm per forage NDF intake linearly decreased (p≤0.005) with increasing CP from both respiration and eructation.</p><p><strong>Conclusion: </strong>These findings provide no clear evidence that increasing the CP content of the concentrate mix from 15.0% to 18.5% DM improves growth performance and body metabolism in finishing Hanwoo steers. Nevertheless, they suggest that a higher CP level may negatively affect feed efficiency.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1934-1942"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-transcriptome RNA sequencing reveals global expression dynamics and ceRNA regulatory networks related to hair follicle development and melanogenesis in goats.","authors":"Junyin Zhao, Jipan Zhang, Ziyi Chhen, Min Xiao, Yongju Zhao","doi":"10.5713/ab.24.0617","DOIUrl":"10.5713/ab.24.0617","url":null,"abstract":"<p><strong>Objective: </strong>Domestic animals, fur is a product of long-term selection by humans and the natural environment. It is generally used to distinguish between different breeds. This study aims to dissect the molecular mechanisms underlying the distinct fur characteristics of goats, particularly focusing on the molecular and regulatory differences between the Dazu Black Goat (DBG) and the Inner Mongolia Cashmere Goat (IMCG). Through whole-transcriptome analysis, we aim to identify differentially expressed RNAs and construct a ceRNA network to reveal the genetic regulation of goat hair follicle development and melanin production.</p><p><strong>Methods: </strong>Skin, hair, and cashmere samples were collected from DBG (n = 15) and IMCG (n = 17) to assess hair follicle density, length, diameter, and melanin content. Whole-transcriptome sequencing of skin tissues from DBG (n = 3) and IMCG (n = 3) identified 50,652 RNAs. Differential expression analysis was performed on mRNAs, lncRNAs, miRNAs, and circRNAs.</p><p><strong>Results: </strong>IMCG exhibited significantly higher hair follicle density, hair length, and cashmere diameter than DBG (p < 0.01), whereas DBG had significantly thicker hair and higher melanin content (p < 0.01). A total of 640 differentially expressed RNAs were identified, including 157 mRNAs, 234 lncRNAs, 72 miRNAs, and 177 circRNAs. These were enriched in pathways related to melanogenesis, hair follicle development, and GO terms such as collagen fiber organization and pigmentation. ceRNA networks constructed from differentially expressed RNAs revealed key regulatory mechanisms of coat color and hair traits.</p><p><strong>Conclusion: </strong>Whole-transcriptome sequencing revealed expression profiles and ceRNA networks involved in hair follicle development and melanogenesis in goats. These findings provide insights into the roles of coding and non-coding RNAs in fur traits, supporting future breeding strategies and textile applications.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1841-1857"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The beneficial impact of pomegranate oil nanoemulsion on the quality of cryopreserved bovine sperm: antioxidant and anti-apoptotic effects.","authors":"Fatma Mohsen Shalaby, Soha A Hassan, Ali A El-Raghi, Fatemah Enad Alajmi, Mohamed G Alfayoumi, Kandil Abd El Hai Attia","doi":"10.5713/ab.25.0067","DOIUrl":"10.5713/ab.25.0067","url":null,"abstract":"<p><strong>Objective: </strong>This study was conducted to investigate the effect of pomegranate oil nanoemulsion (PO-NE) supplementation in semen extender on semen quality, redox status and apoptotic genes of cryopreserved bovine semen.</p><p><strong>Methods: </strong>Seven proven fertility Holstein Friesian bulls (4 to 6 years) were involved, and the semen samples were collected using the artificial vagina method. Semen was pooled and cryopreserved in tris extender containing PO-NE at 0 (PO-NE0), 1 (PO-NE1), 2 (PO-NE 2) and 4 μg/mL (PO-NE4), respectively.</p><p><strong>Results: </strong>Incorporating 2 or 4 μg/mL PO-NE into freezing media significantly increased sperm progressive motility, viability, membrane integrity, and kinematic parameters. Furthermore, the aforementioned two treated concentrations demonstrated superior antioxidative activities (total antioxidant capacity and super oxide dismutase) and higher nitric oxide levels compared to the control group (p<0.05). The levels of hydrogen peroxide, malondialdehyde, and Nuclear Factor-Kappa B were notably lower in the PO-NE4 treated group compared to the control group. The addition of 2 or 4 μg/mL of PO-NE to the freezing media significantly downregulated pro-apoptotic genes (caspase 3, Bax), while significantly induced the expression of anti-apoptotic gene Bcl2. The addition of PO-NE preserved plasma membrane and acrosome integrity and maintained the ultrastructure of sperm, contrasting with PO-NE0, which exhibited the most damage.</p><p><strong>Conclusion: </strong>Supplementing the bovine freezing extender with 2 or 4 μg/mL of PO-NE enhanced post-thawed sperm characteristics by reducing oxidative stress, improving antioxidant indices and apoptotic genes expression, and preserving the ultrastructure integrity of frozen-thawed cattle sperm.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1894-1905"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacillus rhamnosus GR-1 alleviates Escherichia coli-triggered bovine endometrial epithelial cells damage via the reactive oxygen species-mitochondrial pathway.","authors":"Xiaowei Feng, Yan Li, Xiangfu Wen, Mingque Feng, Tianxiong Jin, Muhammad Shahid, Yan Sun, Jiawei Liu, Bei Liu, Jia Cheng, Mingchao Liu","doi":"10.5713/ab.25.0031","DOIUrl":"10.5713/ab.25.0031","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to evaluate the function of the reactive oxygen species (ROS)-mitochondrial pathway in attenuating Escherichia coli (E. coli) induced apoptosis in bovine endometrial epithelial cells (BENDs) by Lactobacillus rhamnosus (L. rhamnosus) GR-1.</p><p><strong>Methods: </strong>The BENDs were exposed to preincubation with and without L. rhamnosus GR-1 for 3 hours (h) and they were later subject to E. coli for 6 h. The release of lactate dehydrogenase (LDH), the expression of oxidative factors, adhesion and invasion of E. coli, the expression of mitochondrial membrane potential (MMP), apoptotic rate and apoptosis-associated protein expression were observed. Then, ROS expression, MMP level and cell rate apoptosis rate were further detected after the intervention of antioxidant n-acetyl-l-cysteine (NAC).</p><p><strong>Results: </strong>L. rhamnosus GR-1 was capable of obviously alleviating the content of LDH, ROS expression, E. coli adhesion and invasion, the apoptotic rate and MDA concentrations in BENDs induced by E. coli (p<0.01). In addition, L. rhamnosus GR-1 could notably promote the levels of antioxidant factors (SOD, GSH, T-AOC) (p<0.01), inhibit the depolarization of MMP (p<0.01), and levels of apoptosis-related factors (Bax, Caspase-3 and Cyt-c), and promote anti-apoptosis factor (Bcl-2) (p<0.01). Moreover, after the intervention of antioxidant NAC, ROS expression, MMP depolarization, and apoptotic rate induced by E. coli in BENDs were decreased (p<0.01).</p><p><strong>Conclusion: </strong>In E. coli-induced apoptosis in BENDs, therapeutic strategies aimed at down-regulating ROS and MDA and up-regulating antioxidant factors may maintain mitochondrial function and provide theoretical support for the use of probiotics in bovine endometritis.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1996-2007"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}