Animal Bioscience最新文献

{"title":"Estimation of genetic parameters for reproductive traits in Korean dairy cattle.","authors":"Jae-Gu Lee, Jeongwoo Seo, Mahboob Alam, Hyungjun Song, Seokhyun Lee, Joohyeon Cho, Chang-Gwon Dang, Joonho Lee","doi":"10.5713/ab.24.0455","DOIUrl":"10.5713/ab.24.0455","url":null,"abstract":"<p><strong>Objective: </strong>In Korea, dairy cattle breeding programs have historically prioritized productive, conformation traits, leading to positive improvements, yet reproductive traits have lagged in development. This study was conducted to develop the breeding program of key reproductive traits in the Korean dairy cattle population.</p><p><strong>Methods: </strong>Utilizing data from 7,596 farms and over seven million observations, we conducted quality control to rectify manual entry errors and selected traits in line with international genetic evaluation standards. Traits analyzed included heifer conception rate (HCR), interval from calving to first insemination (CF), cow conception rate (CCR), interval from first to last insemination (FL), and days open (DO). Genetic parameters were estimated using a single trait animal model for HCR and a multiple lactation animal model for CF, CCR, FL, and DO, considering contemporary group of herd-insemination year, insemination month, and monthly age as fixed effects.</p><p><strong>Results: </strong>Results showed low heritability estimates, ranging from 0.007 to 0.035 across different traits and lactations. Theoretical reliability appears to be low on average due to the influence of heritability, but it showed sufficiently high reliability in some sires (over 0.8). In terms of genetic and phenotypic trends, capacity for reproductive traits declined for a long time until around 2014. In recent individuals, improved trend can be found.</p><p><strong>Conclusion: </strong>This study addressed the critical need for enhancing reproductive efficiency to complement the existing breeding goals, thereby supporting sustained economic viability in the dairy industry. The results underscore the need for improved data quality and methodological adjustments for reproduction records to enhance the genetic evaluation of dairy cattle in Korea.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"33-40"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel equations for estimating gross energy in feed ingredients for non-ruminants.","authors":"Yoon Soo Song, Ah Reum Son, Beob Gyun Kim","doi":"10.5713/ab.24.0312","DOIUrl":"10.5713/ab.24.0312","url":null,"abstract":"<p><strong>Objective: </strong>The present study aimed to evaluate the accuracy of previous equations for estimating gross energy (GE) in feed ingredients and to develop the novel equations.</p><p><strong>Methods: </strong>A total of 2,279 ingredient samples consisted of barley (n = 58), corn (n = 319), corn distillers dried grains with solubles (n = 13), corn gluten feed (n = 583), copra expellers (n = 156), copra meal (n = 234), cottonseed meal (n = 12), palm kernel expellers (n = 504), rapeseed meal (n = 114), soybean meal (n = 138), wheat (n = 70), and wheat bran (n = 78) were analyzed for dry matter (DM), crude protein (CP), ether extract (EE), crude fiber, ash, and GE. The 2,279 ingredient samples were used for evaluating the previous equations and developing novel equations. Using data from 62 ingredients in the swine NRC publication in 2012, the old equations and the novel equations were evaluated.</p><p><strong>Results: </strong>Based on the evaluation using 2,279 samples, the equation developed by Ewan in 1989 underestimates GE by 218 kcal/kg DM (standard error = 4 and p<0.001) on average and underestimates more for low-GE ingredients (linear bias = -0.121; standard error = 0.025 and p<0.001). The equation reported by Sauvant, Perez, and Tran in 2004 also underestimates GE by 135 kcal/kg DM (standard error = 4 and p<0.001) on average. Novel equations for estimating GE concentration (kcal/kg DM) in feeds were developed: GE = 4,299+7×CP +53×EE, with R2 = 0.342 and p<0.001; GE = 4,341+11×CP+54×EE-24×ash, with R2 = 0.372 and p<0.001, where all independent variables are in % DM. In the validation using 62 feed ingredients in the NRC publication, the equations developed in the present study were accurate whereas the previous equations were not.</p><p><strong>Conclusion: </strong>The novel equations developed in the present study fairly accurately estimate gross energy concentrations in concentrate feeds.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"86-91"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of long-term supplementation of a formulated botanical blend on growth performance and carcass traits in feedlot steers.","authors":"Thiago Lauro Maia Ribeiro, Becca B Grimes Francis, Erin R DeHaan, Cassidy R Ross, Justin J Delver, Forest L Francis, Jeff S Heldt, Emma H Wall, Warren C Rusche, Zachary K Smith","doi":"10.5713/ab.24.0125","DOIUrl":"10.5713/ab.24.0125","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to determine if a formulated blend of capsicum oleoresin, clove essential oil, and garlic essential oil (Fytera Advance - Selko USA, Indianapolis IN, USA; CCG) influences measures of cattle growth, efficiency, or carcass traits, during the finishing phase in steers fed a concentrate-based diet.</p><p><strong>Methods: </strong>Charolais×Angus steers (n = 96; initial shrunk body weight [BW] = 391±34.0 kg) were used in a 144-d (16 February 2023 to 9 July 2023) finishing feedlot experiment in Brookings, SD, USA. Steers were individually weighed and allotted to one of 14 pens (6 to 7 steers; 7 pens/treatment) in a randomized complete block design and randomly assigned to 1 of 2 treatments: control diet without the test product (CON) or a diet including CCG at 500 mg/steer daily (CCG). Steers were fed twice daily, and bunks were managed according to a slick bunk system.</p><p><strong>Results: </strong>There were no differences (p≥0.10) in any growth performance outcomes from d 1 to 35, 36 to 70, or 71 to 98. From d 99 to 144 steers from CCG tended to have 5% greater average daily gain (p = 0.09) and 8% improved feed conversion (G:F) (p = 0.01). No differences (p≥0.15) were noted for cumulative growth performance measures. No differences were noted for any carcass measurements or categorical carcass outcomes, nor lung or liver health outcomes (p≥0.15).</p><p><strong>Conclusion: </strong>The use of CCG had no influence on cumulative growth performance responses. However, the use of CCG improved G:F during the late feeding period.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"77-85"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination model for freshness prediction of pork using VIS/NIR hyperspectral imaging with chemometrics.","authors":"Minwoo Choi, Hye-Jin Kim, Azfar Ismail, Hyun-Jun Kim, Heesang Hong, Ghiseok Kim, Cheorun Jo","doi":"10.5713/ab.24.0255","DOIUrl":"10.5713/ab.24.0255","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to develop an enhanced model for predicting pork freshness by integrating hyperspectral imaging (HSI) and chemometric analysis.</p><p><strong>Methods: </strong>A total of 30 Longissimus thoracis samples from three sows were stored under vacuum conditions at 4°C±2°C for 27 days to acquire data. The freshness prediction model for pork loin employed partial least squares regression (PLSR) with Monte Carlo data augmentation. Total bacterial count (TBC) and volatile basic nitrogen (VBN), which exhibited increases correlating with metabolite changes during storage, were designated as freshness indicators. Metabolic contents of the sample were quantified using nuclear magnetic resonance.</p><p><strong>Results: </strong>A total of 64 metabolites were identified, with 34 and 35 showing high correlations with TBC and VBN, respectively. Lysine and malate for TBC (R2 = 0.886) and methionine and niacinamide for VBN (R2 = 0.909) were identified as the main metabolites in each indicator by Model 1. Model 2 predicted main metabolites using HSI spectral data. Model 3, which predicted freshness indicators with HSI spectral data, demonstrated high prediction coefficients; TBC R2p = 0.7220 and VBN R2p = 0.8392. Furthermore, the combination model (Model 4), utilizing HSI spectral data and predicted metabolites from Model 2 to predict freshness indicators, improved the prediction coefficients compared to Model 3; TBC R2p = 0.7583 and VBN R2p = 0.8441.</p><p><strong>Conclusion: </strong>Combining HSI spectral data with metabolites correlated to the meat freshness may elucidate why certain HSI spectra indicate meat freshness and prove to be more effective in predicting the freshness state of pork loin compared to using only HSI spectral data.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"142-156"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intracellular domain of CATSPER1 could serve as a cytoplasmic platform for redox processes in mammalian sperm.","authors":"Jingon Kim, Jae Yeon Hwang","doi":"10.5713/ab.24.0631","DOIUrl":"https://doi.org/10.5713/ab.24.0631","url":null,"abstract":"<p><strong>Objective: </strong>Mammalian sperm acquire fertilizing ability in the female reproductive tract and develop hyperactivated motility, which is indispensable for male fertility. Hyperactivated motility is initiated by Ca2+ influx via the sperm-specific ion channel, CatSper. CATSPER1, a CatSper pore subunit, possesses a long N-terminal intracellular domain and its degradation correlates with unsuccessful sperm migration in the female tract. However, the cellular function and molecular significance of the CATSPER1 N-terminal domain are not well understood. Here, we identify the interactome of the CATSPER1 N-terminal domain and propose a function for the intracellular domain in mammalian sperm.</p><p><strong>Methods: </strong>To identify CATSPER1 N-terminus interactome, we produced recombinant CATSPER1-N-terminus in bacterial system. The purified protein was incubated with testicular lysates and eluted together with testicular interacting proteins. The elutes were subjected to proteomic analysis and CATSPER1-N-terminus interactome was profiled. Identified proteins were further analyzed by functional annotation.</p><p><strong>Results: </strong>We purified the partial CATSPER1 N-terminal domain and identified 57 testicular proteins as domain interactomes using mass spectrometry analysis. Functional annotation analysis revealed that 106 gene ontologies were significantly enriched, 16 of which were related to redox processes. We found that antioxidant enzymes, such as PARK7 and PRDX2, 4, and 6, were included in the enriched redox-related gene ontologies.</p><p><strong>Conclusion: </strong>These results suggest that the CATSPER1 N-terminus could function in defending against oxidative stress to support the successful migration of mammalian sperm to fertilizing sites in the female reproductive tract.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N6-methyladenosine (m6A)-circHECA from secondary hair follicle of cashmere goats: identification, regulatory network and expression regulated potentially by methylation of its host gene promoter.","authors":"Jincheng Shen, Taiyu Hui, Man Bai, Yixing Fan, Yubo Zhu, Qi Zhang, Ruqing Xu, Jialiang Zhang, Zeying Wang, Wenxin Zheng, Wenlin Bai","doi":"10.5713/ab.24.0081","DOIUrl":"10.5713/ab.24.0081","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to identify the N6-methyladenosine (m6A)- circHECA molecule in secondary hair follicles (SHFs) of cashmere goats, and generate its potential regulatory network, as well as explore the potential relationship between transcriptional pattern of m6A-circHECA and promoter methylation of its host gene (HECA).</p><p><strong>Methods: </strong>The validation of circHECA m6A sites was performed using methylation immunoprecipitation (Me-RIP) along with reverse transcription-quantitative polymerase chain reaction (RT-qPCR) technique. The nucleus and cytoplasm localizations of m6AcircHECA were performed using SHF stem cells of cashmere goats with RT-qPCR analysis. Based on in-silico analysis, the regulatory networks of m6A-circHECA were generated with related signal pathway enrichment. The methylation level of promoter region of m6A-circHECA host gene (HECA) was assessed by the bisulfite sequencing PCR (BSPPCR) technique.</p><p><strong>Results: </strong>The m6A-circHECA was confirmed to contain four m6A modification sites including m6A-213, m6A-297, m6A-780, and m6A-927, and it was detected mainly in cytoplasm of the SHF stem cells of cashmere goats. The integrated regulatory network analysis showed directly or indirectly complex regulatory relationships between m6A-circHECA of cashmere goats and its potential target molecules: miRNAs, mRNAs, and proteins. The regulatory network and pathway enrichment indicated that m6A-circHECA might play multiple roles in the SHF physiology process of cashmere goats through directly or indirectly interacting or regulating its potential target molecules. A higher methylation level of promoter region of HECA gene in SHFs of cashmere goats might cause the lower expression of m6A-circHECA.</p><p><strong>Conclusion: </strong>The m6A-circHECA might play multiple roles in SHF physiology process of cashmere goats through miRNA mediated pathways along with directly or indirectly interaction with its target proteins. The promoter methylation of m6A-circHECA host gene (HECA) most likely was implicated in its expression inhibition in SHFs of cashmere goats.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2066-2080"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Samae Dam chicken: a variety of the Pradu Hang Dam breed revealed from microsatellite genotyping data.","authors":"Nivit Tanglertpaibul, Trifan Budi, Chien Phuoc Tran Nguyen, Worapong Singchat, Wongsathit Wongloet, Nichakorn Kumnan, Piangjai Chalermwong, Anh Huynh Luu, Kantika Noito, Thitipong Panthum, Pish Wattanadilokchatkun, Anuphong Payopat, Natthamon Klinpetch, Aingorn Chaiyes, Kanithaporn Vangnai, Chotika Yokthongwattana, Chomdao Sinthuvanich, Syed Farhan Ahmad, Narongrit Muangmai, Kyudong Han, Mitsuo Nunome, Akihiko Koga, Prateep Duengkae, Sompon Waipanya, Yoichi Matsuda, Kornsorn Srikulnath","doi":"10.5713/ab.24.0161","DOIUrl":"10.5713/ab.24.0161","url":null,"abstract":"<p><strong>Objective: </strong>The remarkable adaptability to the environment, high growth rate, meat with good taste and aroma, and ornamental appearance of the Pradu Hang Dam (PDH) and Samae Dam (SD) chickens make them valuable for improvement of poultry production to enhance food security. However, despite their close phenotypic similarity, distinct classification of PDH and SD chickens remains controversial. Thus, this study aimed to clarify genetic origins and variation between PDH and SD chickens, genetic diversity and structures of PDH and SD chickens.</p><p><strong>Methods: </strong>This study analyzed 5 populations of PDH and 2 populations of SD chickens using 28 microsatellite markers and compared with those of other indigenous and local chicken breeds using Thailand's \"The Siam Chicken Bioresource Project\" database.</p><p><strong>Results: </strong>Considerably high genetic variability was observed within PDH (370 total alleles; 4.086±0.312 alleles/locus) and SD chickens (179 total alleles; 3.607±0.349 alleles/locus). A partial overlap of gene pools was observed between SD chickens from the Department of Livestock, Uthai Thani (SD1) and PDH chickens, suggesting a potentially close relationship between the two chicken breeds. A gene pool that partially overlapped with that of the red junglefowl was observed in the SD chicken population from the Sanhawat Farm Uthai Thani population (SD2). Distinct subclusters were observed within SD chickens, indicating the possibility that genetic differentiation occurred early in the process of establishment of SD chickens.</p><p><strong>Conclusion: </strong>These findings could offer valuable insights into genetic verification of Thai local chicken breeds and their sustainable conservation and utilization.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2033-2043"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association study comparison analysis based on Hanwoo full-sib family.","authors":"Ji-Yeong Kim, Eun-Ho Kim, Ho-Chan Kang, Cheol-Hyun Myung, Il-Keun Kong, Hyun-Tae Lim","doi":"10.5713/ab.24.0303","DOIUrl":"10.5713/ab.24.0303","url":null,"abstract":"<p><strong>Objective: </strong>The improvement of carcass traits is essential for the Hanwoo industry because of the Hanwoo grade determination system, and genome-wide association study (GWAS) analysis is an instrumental tool for identifying the genetic factors that impact these traits. While GWAS analysis utilizing family data offers advantages in minimizing genetic bias, research on family-based GWAS in Hanwoo is currently lacking.</p><p><strong>Methods: </strong>This study classified Group A using both parental and offspring genetic information, and Group B based solely on offspring genetic information, to compare GWAS analysis results of Hanwoo carcass traits.</p><p><strong>Results: </strong>A total of 16 significant single nucleotide polymorphism (SNP) markers were identified in Group A, comprising 7 for carcass weight (CWT), 3 for back fat thickness (BFT), and 6 for marbling score (MS). In Group B, 7 significant SNP markers were identified, including 3 for CWT, 1 for eye muscle area, 1 for BFT, and 2 for MS. Functional annotation analysis revealed only one common function related to carcass traits between the groups, while protein-protein interaction analysis indicated more gene interactions in Group A. The reliability of estimated values for common SNP markers identified between the groups was higher in Group A.</p><p><strong>Conclusion: </strong>GWAS analysis utilizing parental genetic information holds greater potential for application, owing to its higher reliability of estimated values and the ability to explore numerous candidate genes.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2054-2065"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Mulberry leaf 1-deoxynijirimycin supplementation shortens villus height and improves intestinal barrier in fattening rabbits.","authors":"Shaocong Li, Tao Li, Zijie Jiang, Wenyu Hou, Qirui Hou, Boris Ramos Serrano, Adileidys Ruiz Barcenas, Yuhua Wang, Weiguo Zhao","doi":"10.5713/ab.24.0109","DOIUrl":"10.5713/ab.24.0109","url":null,"abstract":"<p><strong>Objective: </strong>The current study investigated the effects of mulberry 1-deoxynijirimycin (DNJ) on the digestion ability, intestinal morphology, and intestinal barrier of rabbits.</p><p><strong>Methods: </strong>A total of 36 New Zealand White rabbits (male) about 45 days old (mean body weight of 1.05±0.04 kg) were reared and commercial diets were employed, and afterwards divided into three groups (n = 12) with different levels of DNJ extract additive in feed: T0 (0 g/kg), T1 (0.35 g/kg), T2 (0.7 g/kg) for 28 d.</p><p><strong>Results: </strong>The results demonstrated that T2 decreased the average daily gain (p<0.05). T1 and T2 decreased villus height and inflammatory factor levels as compared with T0 (p<0.05). DNJ significantly decreased the content of valeric acid (p<0.05). The content of acetic acid, propionic acid, iso butyric acid, iso valeric acid in T1 were higher than those in T0 and T2 (p<0.05). The content of butyric acid in T2 was lower than it in T0 and T1 (p<0.05). The content of caproic acid was firstly improved then reduced as the DNJ concentration improved (p<0.05). T2 significantly increased the abundance of dgA-11_gut_group and Christensenellaceae_R-7_group while decreased Bacteroide and Ralstonia as compared with T0 (p<0.05). Compared with T0, T1, and T2 significantly improved the gene expression of JAM2, JAM3, mucin4, mucin6 (p<0.05), T1 significantly decreased the expression of occluding while T2 significantly increased (p<0.05), T2 significantly increased the expression of claudin1 and claudin2 (p<0.05).</p><p><strong>Conclusion: </strong>DNJ at high level changed microbiome compositions, inhibited inflammation, and improved intestinal barrier while it decreased the growth performance and shorted villus height in rabbit jejunum by regulating short chain fatty acid compositions in rabbits.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2101-2112"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of acute and chronic heat stress on the rumen microbiome in dairy goats.","authors":"Min Li, Lian-Bin Xu, Chen Zhang, Pei-Hua Zhang, Sha Tao, Hong-Yun Liu","doi":"10.5713/ab.24.0120","DOIUrl":"10.5713/ab.24.0120","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to reveal the influence of acute and chronic heat stress (HS) on the abundance and function of rumen microbiome and host metabolism.</p><p><strong>Methods: </strong>Forty mid-lactation goats were randomly divided into two artificial environments: control group and heat-stressed group. This study was recorded from two periods, 1 day and 28 days. The first day was defined as control 1 (CT1) and HS 1 (acute HS), and the last day was defined as CT28 and HS28 (chronic HS). On the first and last day, 6 dairy goats in each group were randomly selected to collect rumen liquid after the morning feeding through oral stomach tubes. The barn temperature and humidity were recorded every day.</p><p><strong>Results: </strong>Disruption of the rumen microbiome was observed under chronic HS, represented by an increase in the abundance of Prevotella and Bacteroidales (p<0.05), and upregulation of carbohydrate transport and metabolism functions (p<0.05). Additionally, the abundance of Succinimonas and Ruminobacter in chronic HS is lower than in acute HS (p<0.05), and the functions of intracellular trafficking, secretion and vesicular transport, and the cytoskeleton were downregulated (p<0.05).</p><p><strong>Conclusion: </strong>The HS affected the interaction between the microbiota and host, thereby regulated milk production in dairy goats. These findings increased understanding of the crosstalk between hosts and bacteria.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2081-2090"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}