Animal Bioscience最新文献

{"title":"Mitochondrial morphology and energy metabolism in reprogrammed porcine expanded potential stem cells.","authors":"Yun Ju Lee, Jae Hoon Song, Je Woo Lee, Tae Kyung Hong, Sang Jun Uhm, Kwonho Hong, Jeong Tae Do","doi":"10.5713/ab.24.0521","DOIUrl":"10.5713/ab.24.0521","url":null,"abstract":"<p><strong>Objective: </strong>Expanded potential stem cells (EPSCs) are stem cells that can differentiate into embryonic and extraembryonic lineages, including extraembryonic endoderm and trophoblast lineages. Therefore, EPSCs have great potential in advancing regenerative medicine, elucidating disease mechanisms, and exploring early embryonic development. However, the generation and characterization of EPSCs in pigs have not been thoroughly explored. In this study, we successfully generated porcine EPSCs (pEPSCs).</p><p><strong>Methods: </strong>We reprogrammed porcine fetal fibroblasts (PFFs) using an integration-free method with Sendai virus vectors.</p><p><strong>Results: </strong>The resulting pEPSCs expressed key pluripotency markers and demonstrated the ability to differentiate between embryonic and extraembryonic lineages. Notably, reprogramming into pEPSCs was associated with a transformation of mitochondrial morphology from the elongated form observed in PFFs to a globular shape, reflecting potential alterations in energy metabolism. We observed significant remodeling of mitochondrial morphology and a subsequent shift towards glycolytic energy dependence during the reprogramming of PFFs into pEPSCs.</p><p><strong>Conclusion: </strong>Our findings provide valuable insights into the characteristics of EPSCs in pigs and highlight their potential applications in regenerative medicine, disease modeling, and emerging fields such as cell-based meat production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"444-453"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of processing techniques on energy content and amino acid digestibility in corn germ meal fed to growing pigs.","authors":"Jinbiao Zhao, Zhaoyu Liu, Xiaoming Song, Meiyu Yang, Zhongchao Li, Ling Liu","doi":"10.5713/ab.24.0581","DOIUrl":"https://doi.org/10.5713/ab.24.0581","url":null,"abstract":"<p><strong>Objective: </strong>The study was conducted to determine energy contents, apparent total tract digestibility (ATTD) of nutrient, the apparent (AID) and standardized (SID) ileal digestibility of amino acid (AA) in corn germ meals (CGM) produced by processing technologies of wet milling (CGM-CV1 and CGM-CV2), heating-dried CGM with steep liquor (CGMSL-DH) and dried using indirect heat (CGMSL-IH), corn germ expellers (CGE) and dry-grind processing method (CGM-DG).</p><p><strong>Methods: </strong>In Exp.1, Forty-two crossbred male barrows with an initial body weight (BW) of 51.2 ± 4.5 kg were assigned to 1 of 7 diets in a randomized complete block design. In Exp,2, Seven cannulating barrows with an initial BW of 35.3 ± 1.3 kg were assigned to 7 diets in each period according to a Latin square design. Each diet included 6 replicates.</p><p><strong>Results: </strong>The ATTD of acid detergent fiber (ADF) and gross energy (GE), and SID of most AA in CGMSL-IH was greater (p<0.05) than those in CGM-CV2. The ATTD of organic matter (OM), crude protein (CP) and GE, and SID of most AA in CGMSL-DH was greater (p<0.05) than those in CGM-CV1 for pigs. The ATTD of GE and acid-hydrolyzed ether extract (AEE), contents of digestible (DE) and metabolizable energy (ME), and SID of most AA in CGE were greater (p<0.05) than those in CGM-CV1. The ATTD of OM and GE, and SID of all AA in CGM-DG were greater (p<0.05) than those in CGM-CV1. Compared with the CGM-CV1, CGM-CV2 showed a greater (p<0.05) ATTD of GE and SID of some AA for growing pigs.</p><p><strong>Conclusion: </strong>Different processing technologies lead to large variations on energy contents and amino acids digestibility of corn germ meal for growing pigs. The CGE contains more available energy and CGMSL-DH, CGMSL-IH and CGM-DG provides more ileal digestible AA for growing pigs compared with the CGM-CV1 and CGM-CV2.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of bacteriophage cocktail supplementation in gestation diet on reproductive performance, blood profile, milk composition, and fecal microflora of sows.","authors":"Jinsu Hong, Geon Il Lee, Jae-Cheol Jang, Yoo Yong Kim","doi":"10.5713/ab.24.0796","DOIUrl":"https://doi.org/10.5713/ab.24.0796","url":null,"abstract":"<p><strong>Objective: </strong>The present study aimed to evaluate the effects of bacteriophage cocktail supplementation in gestation sow's diet on gestating sows and their progeny.</p><p><strong>Methods: </strong>A total of 57 F1 multiparous sows (Yorkshire × Landrace) were allotted to one of three treatment groups in a completely randomized design. The sows were fed corn-soybean meal-based diet supplemented with 0, 0.05, or 0.10% of bacteriophage cocktail during the gestation period and they were fed a common diet during the lactation period. Body weight and bacfact thickness were measured throughout the trial along with blood collection for immunoglobulin analysis. Fecal samples were collected on 4, 6, 8, and 10 weeks of gestation period for fecal microflora. Litter performance and milk composition were investigated on 24 hrs postpartum and d 21 of location.</p><p><strong>Results: </strong>Increasing bacteriophage cocktail supplementation levels in gestation diets linearly increased (P < 0.05) feed intake of lactating sows and alive litter weight, and quadratically increased (P < 0.05) litter weight gain from d 0 to 21 of lactation. Dietary treatment did not affect the serum concentrations of immunoglobulin G and immunoglobulin M in the sows. Increasing dietary bacteriophage cocktail levels in gestation diets linearly increased (P < 0.05) the fat content in colostrum. In fecal microflora of sows, the dietary bacteriophage cocktail significantly increased (P < 0.05) the counts of Lactobacillus for d 63 and 105 of the gestation period and decreased the counts of E. coli (P < 0.05) and Salmonella (P < 0.10) for the late gestation period.</p><p><strong>Conclusion: </strong>Supplementation of bacteriophage cocktail at 0.05% in the diet for gestating sows could improve total litter weight and alive litter weight at birth and litter weight gain during the lactation period through a positive change in fecal microflora with an increase of Lactobacillus and decrease of E. coli, and Salmonella.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CIDEB promotes lipid deposition in goat intramuscular adipocytes.","authors":"Zhuohan Huang, Qi Li, Changheng Yang, Changhui Zhang, Lian Huang, Yaqiu Lin, Yong Wang, Hua Xiang, Jiangjiang Zhu","doi":"10.5713/ab.24.0584","DOIUrl":"https://doi.org/10.5713/ab.24.0584","url":null,"abstract":"<p><strong>Objective: </strong>CIDEB (Cell death-inducing DNA fragmentation factor alpha-like effector B), a family member of CIDEs (Cell death-inducing DFF45-like effectors), is well known as a crucial regulator for lipid metabolic signaling pathways in various metabolic tissues and secretory glands. However, its role in regulating intramuscular fat (IMF) deposition in goat remains unclear.</p><p><strong>Methods: </strong>The expression vector pcDNA3.1-CIDEB was constructed and transfected into goat intramuscular preadipocytes; the overexpression and interference efficiency and expression of genes related to lipid metabolism were measured by Real-time PCR; the effect of overexpression of CIDEB and interfering with CIDEB on lipid droplet formation was observed by Oil Red O staining and GPO-Trinder enzymatic reaction. Then RNA-Seq was used to investigate the metabolic pathway of CIDEB affecting adipocyte deposition in goat intramuscular preadipocytes.</p><p><strong>Results: </strong>Overexpression of CIDEB significantly promoted the lipid droplets accumulation and the triglyceride deposition, and significantly upregulated the expression of genes related to lipid metabolism. After overexpression of CIDEB in goat intramuscular preadipocytes, in total 171 differentially expressed genes (DEGs) were found, including 122 up-regulated and 49 down-regulated DEGs, and the top three significantly changed pathways filtered by Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were Cocaine addiction, Amphetamine addiction and Malaria pathways. Conversely, the silencing of CIDEB significantly reduced lipid accumulation in goat intramuscular preadipocytes, meanwhile changed the expression of lipid metabolism genes. For CIDEB silencing, a total of 2140 DEGs were found, including 1252 up-regulated and 888 down-regulated DEGs, and the top three significantly changed pathways filtered by KEGG analysis were Ribosome, Thyroid hormone signaling pathway and Alzheimer disease.</p><p><strong>Conclusion: </strong>The expression of CIDEB can significantly promote lipid deposition of intramuscular adipocytes in goats, and these results provide important data to support further clarifying the mechanism of CIDEB gene on the regulation of intramuscular adipogenesis, and the IMF formation in goat.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular regulatory mechanisms of dietary supplementation with Allium mongolicum Regel powder to improve muscle development and meat quality in Angus calves.","authors":"Wangjing Liu, Chenxu Sun, Huixia Gao, Jianjian He, Aihuan Yu, Yaodi Xie, Haibo Yao, Jiang Hu, Zhaomin Lei","doi":"10.5713/ab.24.0809","DOIUrl":"https://doi.org/10.5713/ab.24.0809","url":null,"abstract":"<p><strong>Objective: </strong>Supplementing animal feed with Allium mongolicum Regel powder (AMRP) additives can promote muscle production and improve meat quality. Here, we explored the effects of dietary AMRP supplementation on the performance, meat quality, and muscle transcriptome profile of Angus calves.</p><p><strong>Methods: </strong>Twelve healthy black Angus calves (female, average body weight = 280.4 ± 15.74 kg, average age = 14 ± 0.6 months) of the same genetic background were randomly assigned to two feed groups: control (CON), basal diet without any supplementation, and AMRP, basal diet supplemented with 20 g of AMRP per calf per day.</p><p><strong>Results: </strong>In general, compared with the control group, dietary AMRP supplementation significantly increased the longissimus thoracis muscle area (p=0.027) and pH24h (p= 0.027) but significantly reduced Warner-Bratzler shear force (p=0.009) and cooking loss (p<0.001). Moreover, 1,284 differentially expressed genes (DEGs) were identified in AMRP-supplemented Angus calves. Pathway analysis revealed that the DEGs were involved in multiple pathways related to muscle development and fat deposition, such as the focal adhesion and MAPK pathways.</p><p><strong>Conclusion: </strong>Dietary supplementation of AMRP improved muscle growth and development in Angus beef cattle, as well as significantly modulated meat quality, and this modulation may affect signaling pathways by influencing the expression of key genes. Our results provide new insights into the development of the meat industry and, most importantly, analyze the mechanism by which AMRP regulates muscle development and improves meat quality at the molecular level.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of whole-plant mulberry supplementation on testis development and antioxidant capacity in Hu rams.","authors":"Jiamei Liu, Ziwei Liang, Wanhong Li, Xiuxiu Weng, Xiangpeng Yue, Fadi Li","doi":"10.5713/ab.24.0627","DOIUrl":"https://doi.org/10.5713/ab.24.0627","url":null,"abstract":"<p><p>This study aimed to investigate the effects of dietary whole-plant mulberry (WM) on testicular development and antioxidant performance in sheep.Fifty-four Hu sheep aged three months were divided into three groups and fed diets containing different proportions of WM (WM0, without WM; WM4, 4% WM; WM8, 8% WM). After a 70-day feeding trial, 15 individuals from each group were humanly slaughtered. The total cholesterol (T-CHO) content and antioxidant capacity of the testes were measured. The expression of functional genes was assessed by RT-qPCR. Proanthocyanidins (PCs) at concentrations of 0, 5, 10, and 20 μM were added to Leydig cells in vitro. The total antioxidant capacity level (T-AOC), testosterone (T) level, cell viability, apoptosis and necrosis ratio were assessed. RNA sequencing was performed to identify the differentially expressed genes (DEGs) between the control (CK) and 10 μM PC groups.The PCs content in the WM was measured at 56.93±2.146 mg/g. The total testicular weight (p=0.06), left testicular weight (p=0.07) and right testicular weight (p<0.05) was increased in the WM8 group compared to the WM0 group. Compared to the WM0 group, the WM8 group showed decreased T-CHO (p<0.05) and increased T-AOC (p<0.05) in the testis, and the expression of StAR, PPARγ and Bcl2 was significantly increased (p<0.05), while Caspase9 and Caspase3 were significantly decreased (p<0.05). In vitro, supplementation of 10 μM PCs in Leydig cells significantly increased cell viability, T-AOC and T levels, and reduced the necrosis ratio (p<0.05) compared to the CK group. Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis showed that the DEGs were significantly enriched in the steroid biosynthesis pathway, p53 signaling pathway, cholesterol metabolism, PPAR signaling pathway, and Hippo signaling pathway.Supplementation with 8% WM improved antioxidant capacity and stimulated testis development by promoting cell proliferation, T synthesis, and antioxidant capacity of Leydig cells.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Dietary Star Anise (Illicium verum) Supplementation during Late Gestation and Lactation on the Performance of Multiparous Sows and Their Progeny until 21 Days Post-weaning.","authors":"Jeong Hyun Moon, Jae-Cheol Jang, Yoo Yong Kim","doi":"10.5713/ab.24.0766","DOIUrl":"https://doi.org/10.5713/ab.24.0766","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the effects of star anise (SA) supplementation during late gestation and lactation on the performance of multiparous sows and their progeny until 21 days post-weaning.</p><p><strong>Methods: </strong>A total of 40 pregnant sows were housed in individual stalls and allocated into two treatment groups using a completely randomized design based on body weight, backfat thickness, and parity. The treatments consisted of either 0% or 0.1% SA supplementation during late gestation and lactation. Following the lactation period, 160 weaning piglets were used to investigate the imprinting effects of SA. The dietary treatment was continued in a manner that the piglets received the same diet as their litter. Data collected included serum antioxidant status in sows, milk composition, and stress markers in piglets, which were measured at various stages.</p><p><strong>Results: </strong>Sows fed with SA during late gestation exhibited significantly higher serum total antioxidant status (p < 0.03). Additionally, SA supplementation increased lactose content in the milk (p < 0.01). Piglets from sows fed the SA diet during late gestation showed lower serum cortisol and epinephrine concentrations at weaning (p < 0.01, p < 0.04, respectively). SA supplementation during lactation further reduced serum cortisol levels in weaning piglets at a day after weaning (p < 0.05).</p><p><strong>Conclusion: </strong>The current experiment indicated that supplementing dietary SA in gestation and lactation diet showed higher serum antioxidant properties in sows and improved the quality of sow milk, consequently enhancing litter performance.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of genetic diversity and inbreeding in the Okinawa indigenous Agu pig through pedigree analysis.","authors":"Shihei Touma, Kyota Fusho, Aisaku Arakawa","doi":"10.5713/ab.24.0646","DOIUrl":"https://doi.org/10.5713/ab.24.0646","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to clarify the changes in genetic diversity and inbreeding over the years as well as to identify the causes of genetic diversity loss using the pedigree of Okinawa indigenous Agu pigs.</p><p><strong>Methods: </strong>The pedigree of the Agu population consisted of 1,997 animals from 1997 to 2020. The equivalent complete generations (ECG), inbreeding coefficient (F), and effective population size (Ne) were computed using ENDOG v4.8. Genetic diversity parameters, including founders (f), the effective number of founders (fe), the effective number of ancestors (fa), and the founder genome equivalent (fge), were derived from the probability of gene origin. The study also investigated changes in genetic diversity indices over time.</p><p><strong>Results: </strong>ECG increased from 0.1 in 2003 to 3.87 by 2020, indicating an improved pedigree depth. The F has been on a downward trend since it peaked at 10.5% in 2015. However, the Ne in the reference population was 14.6, which is below the recommended levels. The f, fe, fa, and fge of the reference population were 86, 26, 19, and 11.5, respectively. These values decrease in the order of f > fe > fa > fge. This indicates that all factors of genetic diversity loss played a role, including the unbalanced contributions of founders, bottlenecks, and random gene losses. Although in recent years efforts have been made to maintain genetic diversity and reduce inbreeding, the Agu pig population exhibited a 5% decrease in genetic diversity over the past 18 years, with both unequal founder contributions and genetic drift identified as significant contributing factors.</p><p><strong>Conclusion: </strong>This study reveals that more favorable breeding strategies (such as optimal contribution and population size expansion) are required to maintain genetic diversity and increase the Ne of the Agu pig population.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of 4-Hydroxy-2,5-dimethyl-3(2H)-furanone supplementation on growth performance, serum antioxidant capacity, rumen fermentation characteristics, rumen bacterial quorum sensing, and microbial community in Hu sheep.","authors":"Chuanpei Fu, Jing Ge, Mingren Qu, Kehui Ouyang, Qinghua Qiu","doi":"10.5713/ab.24.0683","DOIUrl":"https://doi.org/10.5713/ab.24.0683","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the effects of 4-Hydroxy-2,5-dimethyl-3(2H)-furanone on growth performance, serum antioxidant capacity, rumen fermentation characteristics, rumen microbial LuxS/AI-2 quorum sensing, and community composition in Hu sheep.</p><p><strong>Methods: </strong>Twenty-two male Hu sheep with similar body weights and ages were evenly divided into two groups: the control group, which was fed a basal diet (n=11, CON), and the treatment group, which was fed the basal diet supplemented with an additional 1.0 g/d of HDMF (n=11, HDMF).</p><p><strong>Results: </strong>The results showed no significant differences in average daily gain, average dry matter intake, and feed conversion ratio between the HDMF and CON groups (p>0.05), but improvements of 11.12%, 4.55%, and 7.56% were observed, respectively. Compared to the CON group, the HDMF group exhibited increased levels of sglutathione peroxidase and superoxide dismutase, and decreased levels of malondialdehyde and reactive oxygen species, as well as a reduced oxidative stress index (p<0.05). Dietary HDMF supplementation did not affect rumen fermentation characteristics, except for the proportion of valerate (p<0.05). HDMF supplementation increased the AI-2 signaling molecules concentration and biofilm formation in the rumen fluid (p<0.05). There were no significant differences in the richness and evenness of rumen microbes between the HDMF and CON groups. Principal coordinates analysis and nonmetric multidimensional scaling analyses revealed an obvious overlap between the two groups, and ANOSIM also found no significant differences (R=0.0116, p =0.303). Further species annotation and biomarker analysis revealed that the HDMF group reduced the abundances of Shuttleworthia, Eubacterium eligens group, and Erysipelotrichaceae UCG 002, and increased the abundances of Riknellaceae RC9 gut group, Prevotellaceae UCG 003, Desulfobulbus, Methanobrevibacter, and Selenomonas ruminantium.</p><p><strong>Conclusion: </strong>This study suggests that HDMF supplementation enhances the body's antioxidant capacity and increases the abundances of certain disease-resistant bacteria by promoting AI-2 signaling molecules and biofilm formation, thereby ultimately improving the growth performance.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal dynamics of plasma bile acids and its associations with physiological parameters and fecal microbiome during the transition period in dairy cows.","authors":"Feixiang Fan, Liang Chen, Huizeng Sun, Jianxin Jianxin Liu, Kailun Yang, Fengfei Gu","doi":"10.5713/ab.24.0628","DOIUrl":"https://doi.org/10.5713/ab.24.0628","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study is to investigate the dynamic changes of plasma bile acids (BA) and their associations with physiological metabolisms and fecal microbiome in transitional dairy cows.</p><p><strong>Methods: </strong>Twenty multiparous dairy cows were selected, the blood and fecal samples were collected at d -21, -7, +7, and +21 relative to calving. The targeted metabolome and 16s rDNA gene sequencing were applied to detect BA profiles and fecal microbial composition, respectively.</p><p><strong>Results: </strong>Totally, 32 BA species including 9 primary BAs (PBA) and 23 secondary BAs (SBA) were identified. Most of the PBAs (7 out to 9) and SBAs (15 out to 23) exhibited significant increases postpartum compared to prepartum. Notably, ursodeoxycholic acid, taurocholic acid and 7-ketodeoxycholic acid showed higher importance. Correlation analysis showed the BAs concentrations positively correlated with the concentrations of aspartate aminotransferase, total antioxidant capacity, and glutathione peroxidase, but negatively correlated with the concentrations of triglyceride significantly. A decline in bacterial alpha diversity in postpartum and significantly different β-diversity were observed. Additionally, 30 significant different genera were identified over the transition period. Among these, six and eleven biomarkers such as Alistipes and Ruminococcaceae_UCG_014 were identified at +7d and +21d, respectively. Furthermore, the abundances of choloylglycine hydrolase and 7-alpha-hydroxysteroid dehydrogenase involved in SBA biosynthesis were significantly higher postpartum as determined by PICRUSt2 analysis over the transition period.</p><p><strong>Conclusion: </strong>These findings underscore a significant surge in the demand for BAs for postpartum dairy cows and highlight the potential impact of BAs on bovine health. By shedding light on these metabolic dynamics, our study offers valuable insights into strategies for optimizing the nutrition and well-being of perinatal dairy cows.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}