Animal Bioscience最新文献

{"title":"Enhancing the purity and intrinsic properties of ovine dermal papilla cells through flow cytometry sorting and cellular interactions.","authors":"Yuan Gou, Tingyan Hu, Lijuan Zhu, Xiaoyang Lv, Yutao Li, Rui Su, Zhenghai Song, Shanhe Wang, Wei Sun","doi":"10.5713/ab.24.0805","DOIUrl":"10.5713/ab.24.0805","url":null,"abstract":"<p><strong>Objective: </strong>Dermal Papilla Cells (DPCs) play a crucial role in regulating hair follicle development and serve as a valuable in vitro model for screening and analyzing the genes associated with this process. However, current methods for isolating ovine DPCs primarily rely on mechanical techniques, which present several limitations. The aim of this study is to establish a method for isolating and culturing ovine DPCs with high purity and retaining their intrinsic properties.</p><p><strong>Methods: </strong>We identified sheep DPC membrane-specific genes using single-cell transcriptomic data, validated by immunostaining and flow cytometry. Antibody-labeled DPCs were isolated, cultured, and assessed via fluorescence-activated cell sorting (FACS), comparing their purity with conventional mechanical isolation. Mechanically isolated and flow-sorted DPCs were analyzed through agglutination, cell counting kit (CCK-8), and EDU staining. Furthermore, we examined the biological properties of isolated DPCs in conditioned media using CCK-8, EDU, and quantitative reverse transcription polymerase chain reaction assays.</p><p><strong>Results: </strong>PDGFRA was identified as a marker for ovine DPCs. Flow cytometry showed that PDGFRA-labeled DPCs made up 1.54% of the hair follicle cell population, with 1.92% live DPCs obtained via FACS. The isolated DPCs demonstrated agglutination and were positive for ALP, Versican, and α-SMA. Antibody labeling yielded higher DPC purity compared to mechanical isolation, highlighting its efficiency. Accordingly, the addition of conditioned media from mechanically isolated DPCs significantly enhanced agglutination, cell viability, proliferation, and inductive capacity of the sorted DPCs. However, as the number of passages increased, the sorted DPCs demonstrated significant disadvantages in cell agglutination, proliferation rate, and viability compared to mechanically isolated DPCs. Accordingly, the addition of conditioned media from mechanically isolated DPCs significantly enhanced agglutination, cell viability, proliferation, and inductive capacity of the sorted DPCs.</p><p><strong>Conclusion: </strong>This study highlights the effectiveness of antibody labeling and flow cytometry for isolating functionally pure DPCs, as well as the potential of conditioned media to maintain the functional properties of these cells.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1342-1355"},"PeriodicalIF":2.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic signal selection analysis reveals genes related to the lambing trait of Hotan sheep.","authors":"XinKun Wang, Wei Li, QiaoYan Huang, HuiPing Sun, LeXiao Zhu, RuoHuai Gu, Feng Xing","doi":"10.5713/ab.24.0336","DOIUrl":"10.5713/ab.24.0336","url":null,"abstract":"<p><strong>Objective: </strong>Lambing in ewes is a complex and crucial aspect of sheep production that directly influences economic viability and production efficiency. In the present study, we analyzed the genomes of single lamb (SLE) and twin lamb (TLE) Hotan sheep to elucidate the genetic mechanisms underlying lamb production in Hotan sheep.</p><p><strong>Methods: </strong>In this study, we used genome-wide resequencing to analyze the genomes of Hotan sheep exhibiting SLE and TLE traits. To identify the population genetic structure and linkage disequilibrium associated with SLE and TLE traits, we employed two complementary genome selection signals: the interpopulation genetic differentiation index (FST) and nucleotide diversity. Subsequently, we performed gene annotation and enrichment analyses of the selected regions of the obtained genome.</p><p><strong>Results: </strong>Our analysis generated 801 Gb of sequence data, from which 31,864,651 highquality single nucleotide polymorphic loci were identified. We identified 290 selected regions and 332 genes across the Hotan sheep genome by using two widely adopted selective scanning detection methods (FST statistics and Piratio). Functional annotation and enrichment analysis of these genes identified 13 genes associated with the lambing rate, which were enriched in pathways such as the transforming growth factor-β signaling pathway (BMPR2, ID2, SMAD7, THBS1, and RBX1), renal cell carcinoma (PAK1, ELOC), inositol phosphate metabolism (PLC), non-homologous terminal junction (RAD5), ABC transporters (ABCC4), and the NET pathway (H2B, H4, and H2A).</p><p><strong>Conclusion: </strong>This study employed selective elimination analysis to identify candidate genes involved in the regulation of lambing trait in Hotan sheep. By investigating the molecular mechanisms underlying lambing rate in Hotan sheep, we developed molecular markers for twin lambing to enhance reproductive performance and promote the conservation and development of outstanding genetic resources in local Xinjiang sheep.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1384-1397"},"PeriodicalIF":2.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of active components from Piper sarmentosum on the growth, intestinal barrier function, and immunity of broiler chickens.","authors":"Luli Zhou, Guanyu Hou, Hanlin Zhou, Khaled Abouelezz, Yuxiu Ye, Dingfa Wang","doi":"10.5713/ab.24.0736","DOIUrl":"10.5713/ab.24.0736","url":null,"abstract":"<p><strong>Objective: </strong>Pellitorine (PT) and vitexin-2-O-rhamnoside (VR) are two bioactive compounds found in Piper sarmentosum (PS). Their contents are relatively high in the ethanol extract of PS (PSE). However, it remains unknown whether PT and VR are the primary components of PS that exert beneficial effects on gut health. In this study, we aimed to confirm that these two compounds are the primary anti-inflammatory active ingredients in PSE.</p><p><strong>Methods: </strong>Total of 300 female one-day-old Danzhou chickens were randomly divided into five groups with five replicates each. Chickens were given a basal diet (CON group), a basal diet with added PSE (200 mg/kg), VR (1.321 mg/kg), PT (0.563 mg/kg), and a combination of VR+PT (1.321 mg/kg VR and 0.563 mg/kg PT) until they reached 35 days of age.</p><p><strong>Results: </strong>The findings reveal that the VR+PT group exhibited increased liver, thymus and spleen indices compared to the CON group, along with elevated mRNA levels of ZO-1 and Claudin-1 (p<0.05). In contrast, the VR+PT group exhibited reduced serum levels and the ileum mucosa mRNA expression levels of IL-1β and IL-6 compared to the CON group (p<0.05). Additionally, the chickens in the VR+PT group had a greater final weight and average daily gain than those in the CON and PSE groups, with a significantly lower level of D-lactic acid in serum (p<0.05). The serum IgM level increased significantly (p<0.05) in the VR+PT group compared to the PSE group, while the kidney epinephrine level decreased significantly (p<0.05).</p><p><strong>Conclusion: </strong>The present study provides preliminary evidence that VR and PT are two of the main active compounds in PSE, which can cooperatively improve growth performance, intestinal barrier integrity, and immune function in chickens.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1522-1534"},"PeriodicalIF":2.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic correlation analysis of calving ease and gestation length of Korean Holstein cattle.","authors":"Mahboob Alam, Jae-Gu Lee, Chang-Gwon Dang, Seung-Soo Lee, Sang-Min Lee, Ha-Seung Seong, Mina Park, JaeBeom Cha, Eun-Ho Kim, Hyungjun Song, Seokhyun Lee, Joonho Lee","doi":"10.5713/ab.24.0431","DOIUrl":"10.5713/ab.24.0431","url":null,"abstract":"<p><strong>Objective: </strong>To investigate genetic correlation between calving ease (CE) and gestation length (GL) traits of Korean Holstein cattle to understand genetic structures of these two traits and their potential implications.</p><p><strong>Methods: </strong>Records of progenies from first parity (P1, N = 117,921) and second parity (P2, N = 141,104) Holsteins cows were used for analysis. All phenotypes (CE and GL) were considered as calf traits. The CE was an ordered categorical trait. It was scored from 1 (normal calving) to 4 (difficult calving). GL observations were restricted between 260 and 305 days. Variance components and genetic parameters were estimated through a bivariate animal model with a correlated maternal effect using the BLUPF90+ software package.</p><p><strong>Results: </strong>Heritability (h2) estimates of CE for direct and maternal effects were low (less than 0.01) in all parity calves. For GL, despite lower h2 of maternal effect (~0.03), the direct effect was moderately heritable (0.20 to 0.23) in this study. Direct and maternal effects of CE trait were weakly correlated (P1: 0.09±16.60, P2: -0.04±0.00). GL had similar correlation patterns (P1: 0.03±0.00; P2: -0.15±0.05) across parities. Direct genetic correlations of GL and CE were mostly weak (P1: 0.18±0.31; P2: -0.01±0.06), whereas maternal genetic correlations were moderate and positive (P1: 0.39±0.95; P2: 0.46±0.04). Although the genetic influence of GL on CE was not entirely clear due to large estimation errors for parameters, overall positive associations between direct effects and maternal effects essentially indicate a selection potential for GL as an indicator trait of CE.</p><p><strong>Conclusion: </strong>This is the first genetic correlation investigation of GL and CE in Korean Holstein cattle. It provides important insights into genetic architectures of GL and its future potential as an indicator trait for CE improvements in Korean Holsteins.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1305-1315"},"PeriodicalIF":2.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined transcriptome and metabolome analysis of chicken follicles in Tengchong Snow Chicken follicle selection.","authors":"Yanli Du, Xiannian Zi, Kun Wang, Jinshan Ran, Hanyi Xiang, Li Lei, Yong Liu","doi":"10.5713/ab.24.0861","DOIUrl":"10.5713/ab.24.0861","url":null,"abstract":"<p><strong>Objective: </strong>The development of pre-hierarchical follicles (PHFs), especially small yellow follicles (SYFs), directly affects the recruitment of dominant follicles and subsequently affects the egg-laying performance of chickens. The development of PHFs, especially SYFs, and their regulatory mechanisms remain unclear.</p><p><strong>Methods: </strong>Transcriptomic and metabolomic analyses were conducted on large white follicles (LWFs) and SYFs in chickens.</p><p><strong>Results: </strong>Transcriptome sequencing revealed 258 differentially expressed genes (DEGs) between SYFs and LWFs, with 172 genes upregulated and 86 downregulated. The DEGs were mapped to 17 Kyoto encyclopedia of genes and genomes (KEGG) pathways, including glutathione metabolism, ferroptosis, calcium signaling pathway, and neuroactive ligand- receptor interactions, among others. The metabolome analysis revealed 129 significant differential metabolites (DMs), comprising 36 upregulated and 93 downregulated metabolites. The DMs were associated with nine KEGG pathways, including glutathione metabolism, alpha-linolenic acid metabolism, and linoleic acid metabolism, etc. The combined transcriptional and metabolic analysis revealed significantly enriched pathways. Five KEGG pathways associated with follicular development were identified, including glutathione metabolism, ferroptosis, alpha-linolenic acid metabolism, linoleic acid metabolism, and pyrimidine metabolism.</p><p><strong>Conclusion: </strong>Glutathione metabolism may directly inhibit Ferroptosis, which can induce apoptosis of granulosa cells, thereby regulating SYFs development in chickens. These findings could serve as a reference for improving the egg-laying performance of Tengchong snow chickens.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1316-1327"},"PeriodicalIF":2.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of genetic parameters and genome-wide association study for carcass traits in native chickens.","authors":"Xianghua Zhu, Houxue Cui, Nanxi Dong, Lu Liu","doi":"10.5713/ab.25.0070","DOIUrl":"10.5713/ab.25.0070","url":null,"abstract":"<p><strong>Objective: </strong>Global consumption for chicken meat is steadily increasing. Carcass traits (e.g., slaughter weight [SW], eviscerated weight [EW]) are important economic traits for the meat production in chickens. Detection of genetic variants for these traits contributes to elucidating the genetic mechanism and accelerating genetic improvement.</p><p><strong>Methods: </strong>In this study, we performed a genome-wide association study on SW, EW, and thigh muscle weight (ThW), as well as the relative weight of these traits in 565 Sanhuang (SH) chickens. Additionally, genetic estimation has been conducted based on wholegenome variants for carcass traits. Finally, we compared the expression abundance of candidate genes to validate the function on carcass traits.</p><p><strong>Results: </strong>Except the percentage of SW (SP, 0.08), other traits were detected with moderate to high heritability (0.20-0.50). A high genetic correlation (0.72-0.93) was found among the carcass traits (SW, EW, and ThW). A total of 311 single-nucleotide polymorphisms and 73 candidate genes (e.g., IGF2BP1, BMP3, ACSL5) were identified significant association with carcass traits. IGF2BP1 was detected a causal role in EW and ThW, GIP, SNF8, and PHOSPHO1 were also located within the same genomic peak. CCND2 was related to SP and significantly expressed in commercial broilers and SH chickens. Lipid metabolism and immune function were simultaneously altered as a result of the selection for carcass traits. The genes ACSL5 and RASGEF1B were also significantly up-regulated in breast muscle in commercial broilers compared to SH chickens. Additionally, the transcriptomic profile of 73 candidate genes was constructed in multi-tissues, and a total of 17 genes (e.g., IGF2BP1, RASGEF1B, BMP3) were defined as differentially expressed genes between commercial broilers and SH chickens.</p><p><strong>Conclusion: </strong>In general, the findings in this study could facilitate the understandings of genetic mechanisms of carcass traits in chickens, and provide important variants and genes set for genomic breeding.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1328-1341"},"PeriodicalIF":2.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of trehalose supplementation on ruminal microbiota and productivity of Japanese Black heifers under heat-stressed conditions.","authors":"Yasuhiro Morita, Akihisa Mukaiyama, Seiji Inoue, Kazuhisa Mukai, Shuichi Matsuyama, Satoshi Ohkura","doi":"10.5713/ab.24.0468","DOIUrl":"10.5713/ab.24.0468","url":null,"abstract":"<p><strong>Objective: </strong>Hot environments negatively affect cattle productivity, and global warming also causes heat stress, thereby adversely impacting cattle production. Improving cattle management under such conditions is an urgent issue. Trehalose can ameliorate volatile fatty acid production and the diversity of ruminal microbiota in dairy cattle. However, no studies have evaluated on Japanese beef heifers. In this study, we investigated the effects of trehalose supplementation on the ruminal microbiota and productivity of Japanese Black heifers under heat-stressed conditions.</p><p><strong>Methods: </strong>Six cyclic Japanese Black heifers were divided into two groups: control and trehalose supplemented. The ruminal microbiota, pH, and volatile fatty acid production of these heifers were analyzed over 10 weeks in the summer in central Japan.</p><p><strong>Results: </strong>During the experimental period, the heifers in the control group showed significantly higher concentrations of ruminal acetic and propionic acids than those in the trehalose-supplemented group (p<0.05, two-way repeated measures analysis of variance [ANOVA]). The acetic/propionic acid ratio showed no significant difference between the two groups. The alpha diversity in the ruminal bacterial biota in the trehalose supplemented group was higher than that in the control group (p<0.05, two-way repeated measures ANOVA) along with a change in the beta diversity of the ruminal fungal biota (p<0.05, PERMANOVA). Linear discriminant analysis effect size analysis in ruminal microbiota identified specific microorganisms in the control and the trehalosesupplemented samples: 4 and 13 in bacteria; each one fungus; 5 and 4 protozoan families, respectively.</p><p><strong>Conclusion: </strong>Trehalose supplementation in the summer improved ruminal microbiota, especially the types of ruminal bacteria and fungi related to carbohydrate digestion, and maintained the balance of ruminal volatile fatty acid production in Japanese Black heifers. Therefore, trehalose supplementation in feed could improve cattle production under heatstressed conditions and in global-warming scenarios.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1446-1458"},"PeriodicalIF":2.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative effects of Dracocephalum moldavica L. and probiotic on performance and health parameters of broiler chickens challenged by dexamethasone-induced stress.","authors":"Seyed Heidar Hayatolgheibi, Mahdi Hedayati, Saeed Khalaji, Hossein Rajaei-Sharifabadi, Ahmad Reza Seradj","doi":"10.5713/ab.24.0698","DOIUrl":"10.5713/ab.24.0698","url":null,"abstract":"<p><strong>Objective: </strong>Environmental stressors negatively affect poultry productivity by increasing oxidative stress levels. This study evaluated the performance and health status of broilers supplemented with either the medicinal plant Dracocephalum moldavica L. powder (DP) or a commercial probiotic (Lactofeed) in the context of oxidative stress induced by dexamethasone.</p><p><strong>Methods: </strong>A total of 300 one-day-old chicks were enrolled in a completely randomized design with a factorial arrangement of six treatments, in each consisting five replicates of 10 chicks. The experimental treatments were: 1) basal diet without feed additives and no challenge, 2) basal diet supplemented with 0.01% Lactofeed, 3) basal diet with 0.4% DP, 4) basal diet and challenged with dexamethasone (2 mg/kg body weight), 5) basal diet with 0.01% Lactofeed and challenged with dexamethasone, and 6) basal diet with 0.4% DP and challenged with dexamethasone. Growth performance, carcass traits, and internal organ weights were evaluated. Gastrointestinal pH and ileum histology were assessed on days 28 and 42, while cecal microbial counts were determined on days 14, 28, and 42. Serum samples collected on days 14, 28, and 42 analyzed for antibody titers against Newcastle disease and avian influenza viruses, along with white blood cell counts.</p><p><strong>Results: </strong>The dexamethasone challenge negatively impacted performance, certain carcass traits, white blood cell count, and ileum villus height (p = 0.039), while it reduced cecal Escherichia coli and coliform counts (p = 0.001). It suppressed humoral immune response to the Newcastle disease virus on day 28, whereas on day 42, it enhanced the response. Lactofeed and DP additives significantly improved feed intake, body weight gain, white blood cell count, intestinal histology, and microbiota, largely irrespective of dexamethasone challenge.</p><p><strong>Conclusion: </strong>While dexamethasone challenge induced various physiological stresses in broilers, both Lactofeed and DP feed additives demonstrated potential in mitigating these effects, improving overall performance and gut health parameters regardless of stress conditions.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1508-1521"},"PeriodicalIF":2.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles as an alternative treatment for bovine mastitis-A review.","authors":"Beatriz Elena Castro-Valenzuela, Moisés Armides Franco-Molina, Cristina Rodríguez-Padilla","doi":"10.5713/ab.24.0590","DOIUrl":"10.5713/ab.24.0590","url":null,"abstract":"<p><p>Nanotechnology is causing revolutionary changes in different disciplines such as food, pharmacology, medical, and agriculture. Nanotechnology allows for the development of materials or devices with unique physical, chemical, and biological properties that differ from their precursor materials, such as nanoparticles. These have gained significant relevance in veterinary medicine, with the potential to be the future treatment for bovine mastitis. Losses in the global dairy industry of up to USD 35 billion annually caused by this pathology have been determined. Despite advances in the genetic selection of dairy cattle and management practices, the control of bovine mastitis remains inadequate. Antibiotics have been the most prevalent treatment for this condition. However, the residues of the antibiotics in milk compromises its quality. Furthermore, the emergence of bacterial resistance has necessitated the search for alternative strategies for treating mammary gland infections, opening a field of opportunities for the nanoparticle-based therapy. This review addresses key topics to elucidate the potential use of these nanoparticles as a treatment strategy for bovine mastitis.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1291-1304"},"PeriodicalIF":2.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of biopolymer infusion on quality traits of pork sausages during storage.","authors":"Sang-Keun Jin, Donggyun Yim","doi":"10.5713/ab.24.0743","DOIUrl":"10.5713/ab.24.0743","url":null,"abstract":"<p><strong>Objective: </strong>We aimed to verify the impacts of cellulose/chitosan addition on the physicochemical properties of sausages.</p><p><strong>Methods: </strong>Sausages were prepared using 3% cellulose or chitosan and compared with those without biopolymers (control).</p><p><strong>Results: </strong>Sausages containing chitosan exhibited decreased pH values (p< 0.05), whereas those containing cellulose exhibited increased pH values. 2-thiobarbituric acid reactive substances values did not differ between the control and biopolymer samples. The biopolymer samples had lower volatile basic nitrogen contents than the control samples after 4 weeks of storage (p<0.05). The addition of chitosan significantly reduced the microbiological counts of sausages. Moreover, the infusion of chitosan led to lower lightness (L*) and whiteness (W) values but higher redness (a*), yellowness (b*), chroma (C*), and hueangle (h°) values (p<0.05). During storage, the sausages containing chitosan exhibited significantly better textural parameters, except for springiness and gumminess.</p><p><strong>Conclusion: </strong>In conclusion, adding chitosan could reduce microbial growth and prolong the expiration date of sausages without compromising quality.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1535-1542"},"PeriodicalIF":2.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}