Integrated Peptidogenomics Decoding Yak Non-Conventional Peptides: Functional Mapping and Biopotential Mining of Genetic Resources.

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Jingyun Chen, Lu Yang, Weilu Zhang, Yili Liu, Yong Yong Wei, Li Wang, Mingfeng Jiang, Biao Li
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引用次数: 0

Abstract

Objective: Non-conventional peptides, have been found to play a key role in basic plant and animal biological processes. This study aims to investigate from the perspective of NCPs to reveal the adaptive molecular genetic mechanisms of yaks.

Methods: We established an integrated peptidogenomic pipeline, constructed a customized six-frame translation database using high-throughput mass spectrometry, and applied it to the large-scale identification of NCPs in several vital organs/tissues of yaks.

Results: Compared to conventional peptides, these NCPs exhibit unique properties derived from introns, UTRs, out-of-frame exons, and intergenic regions. Additionally, our findings indicate that translation events are more prevalent in unannotated transcripts than previously believed. Combined with transcriptome analysis and ribosome mapping analysis, 727 NCPs were determined to be derived from lncRNA and 944 NCPs were determined to be derived from circRNA. Interestingly, the number of hydrophobic amino acids in NCPs was found to be higher than that of hydrophilic amino acids in almost all tissues; whereas for CPs the results were reversed. These findings suggest their potential involvement in maintaining protein stability and combating oxidative stress. Furthermore, the in vitro antioxidant activity of the 38 candidate peptides further confirmed their physiological functions; however, specific physiological mechanisms require further investigation.

Conclusion: In conclusion, this study demonstrates that a substantial portion of the yak genome can be translated into molecules with biological functions which is highly significant for functional genome research, and these unique hidden molecules will provide basic data for future biomedical development and plateau disease treatment.

整合肽基因组学解码牦牛非常规肽:功能定位与遗传资源生物潜力挖掘。
目的:非常规肽在植物和动物的基本生物过程中发挥着重要作用。本研究旨在从ncp的角度探讨牦牛的适应性分子遗传机制。方法:建立完整的肽基因组学管道,利用高通量质谱技术构建定制化的六帧翻译数据库,并将其应用于牦牛几种重要器官/组织中ncp的大规模鉴定。结果:与传统肽相比,这些ncp表现出来自内含子、utr、框外外显子和基因间区域的独特特性。此外,我们的研究结果表明,翻译事件在未注释的转录本中比以前认为的更为普遍。结合转录组分析和核糖体定位分析,确定727个ncp来源于lncRNA, 944个ncp来源于circRNA。有趣的是,在几乎所有组织中,ncp中疏水氨基酸的数量都高于亲水氨基酸的数量;而对于CPs,结果正好相反。这些发现表明它们可能参与维持蛋白质稳定性和对抗氧化应激。此外,38个候选肽的体外抗氧化活性进一步证实了它们的生理功能;然而,具体的生理机制需要进一步研究。结论:本研究表明,牦牛基因组有相当一部分可以翻译成具有生物学功能的分子,这对功能基因组的研究具有重要意义,这些独特的隐藏分子将为未来生物医学的发展和高原疾病的治疗提供基础数据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
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