Animal Bioscience最新文献

{"title":"Phytase and nutrient-energy matrix: a strategic approach to enhancing the performance of broiler chickens fed a corn-soybean meal-based diet.","authors":"Pok Su Choi, Habeeb Tajudeen, Jun Young Mun, Sang Hun Ha, Abdolreza Hosseindoust, Serin Park, Hye In Park, Priscilla Neves Silvestre, Anushka Lokhande, Santosh Ingale, Jin Soo Kim","doi":"10.5713/ab.24.0565","DOIUrl":"10.5713/ab.24.0565","url":null,"abstract":"<p><strong>Objective: </strong>This study examined the effects of a nutrient matrix with or without phytase on the performance of broiler chicken.</p><p><strong>Methods: </strong>A total of 2,000 day-old Ross 308 broiler chickens were assigned to 5 dietary treatments, with 10 broilers per replicate and 40 replicates per treatment. The experimental diets included 1. CON: A corn and soybean meal (SBM)-basal diet without phytase. 2, NC1: A corn-SBM-based diet with reduced nutrients, specifically 0.13% less phosphorus, 40 kcal/kg less metabolizable energy (ME), and 0.30% less crude protein (CP), without phytase. 3, NC1+PHYT: NC1+500 FTU/kg phytase. 4, NC2: Another corn-SBM-based diet with greater nutrient reductions, including 0.16% less phosphorus, 55 kcal/kg less ME, and 0.45% less CP, without phytase. 5, NC2+PHYT: NC2+1,000 FTU/kg phytase.</p><p><strong>Results: </strong>In the pre-starter and overall phase, feed conversion ratio (FCR) was higher in NC2 and NC2+PHYT. In the starter phase, body weight gain (BWG) was lower in NC2 and NC2+PHYT. In the grower phases, BWG was lower in NC2, while FCR was higher. At d28, the digestibility of ash was higher in NC1+PHYT, while the digestibility of Ca and phosphorus were higher in NC1+PHYT and NC2+PHYT. At day 42, the digestibility of ash, Ca, and phosphorus were higher in NC1+PHYT and NC2+PHYT. The level of tibia ash was lower in NC2. The level of myo-inositol was lower in NC2 at d28, while the level of myo-inositol at d42 was lower in NC1 and NC2.</p><p><strong>Conclusion: </strong>We concluded NC1+PHYT showed a higher growth performance comparable to CON, as against the lower performance observed in NC2, NC2+PHYT, and NC1.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"522-529"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplementation with mulberry leaves improves growth performance and meat quality of Xiangdong black goats.","authors":"Yang Luo, Shuai Gao, Ao Sun, Jianbo Li, Haobang Li, Kangle Yi, Renke Hu, Bin Yang","doi":"10.5713/ab.24.0427","DOIUrl":"10.5713/ab.24.0427","url":null,"abstract":"<p><strong>Objective: </strong>Mulberry (Morus alba) leaf (ML) is a high-quality feed source for ruminants, while it is unclear whether it can enhance the growth performance and meat quality of Xiangdong black goats.</p><p><strong>Methods: </strong>In this study, we investigated the effects of ML supplementation (0%, 5%, 10%, 15%, and 20%) on the growth performance, serum variables, and the profiles of amino acids and fatty acids in the muscle of Xiangdong black goats.</p><p><strong>Results: </strong>Results showed that the final body weight, initial and final dry matter intake, and average daily gain increased linearly and quadratically with the increasing ML content (p<0.05). The serum concentrations of total antioxidant capacity (T-AOC) increased linearly, while immunoglobulin G (IgG) increased quadratically with the increasing ML content (p<0.05). Conversely, the saturated fatty acids (SFA) content in meat decreased linearly with the increasing ML content (p<0.05). Compared to goats without ML supplementation, goats fed with 15% ML showed significant increases in serum concentrations of T-AOC, superoxide dismutase, catalase, and IgG (p<0.05). Furthermore, goats fed with 20% ML displayed significant decreases in SFA (C18:0) content, compared to goats without ML supplementation (p<0.05).</p><p><strong>Conclusion: </strong>These results suggest that ML supplementation promotes the growth performance of goats. A diet containing 15% ML showed better effects in promoting antioxidant and immunomodulatory activities, while a diet with 20% ML was more effective in enhancing meat flavor in Xiangdong black goats.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"466-474"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cupric citrate supplementation improves growth performance, nutrient utilization, antioxidant capacity, and intestinal microbiota of broilers.","authors":"Xuezhuang Wu, Yahao Zhou, Zhentao Lu, Yunting Zhang, Tietao Zhang, Qingkui Jiang","doi":"10.5713/ab.24.0382","DOIUrl":"10.5713/ab.24.0382","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to examine the impact of cupric citrate on broilers and compare it with the copper sulfate groups and a control group.</p><p><strong>Methods: </strong>A total of 360 1-day-old Ross 308 broilers were randomly assigned into 5 groups, each with 6 replicates of 12 broilers per treatment. The control group was fed a basal diet without any copper supplementation. In contrast, the other groups received basal diets supplemented with either 50 mg/kg (CS-50) or 100 mg/kg (CS-100) of copper in the form of copper sulfate, or 50 mg/kg (CC-50) or 100 mg/kg (CC-100) of copper in the form of cupric citrate, for a period of 42 days.</p><p><strong>Results: </strong>The results showed that copper supplementation affected the average daily gain (ADG) from day 1 to 21 (p = 0.026) and day 1 to 42 (p = 0.025) in a source-dependent manner. Copper source also influenced the energy digestibility (p = 0.004), with the CC-100 being the most effective treatment. Notably, birds in the CC-100 groups had significantly reduced concentrations of Escherichia coli (p<0.05) in the cecum, and the Lactobacillus in the ileum, compared to the control group. Dietary copper supplementation also increased the pH in the duodenum (p<0.05) irrespective of the sources and levels. In addition, the source of copper affected the activities of ceruloplasmin (p = 0.014) and CuZn superoxide dismutase (p = 0.025) in the serum, with the CC-100 group showing the highest levels of both enzymes.</p><p><strong>Conclusion: </strong>Copper supplementation generally improves the growth, nutrient utilization, intestinal microflora, gastrointestinal pH, and antioxidant defences of broilers. Moreover, cupric citrate is as effective as copper sulfate even at equal or lower concentrations.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"530-538"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How do crate materials impact the winter transport of broilers?","authors":"Myunghwan Yu, Elijah Ogola Oketch, Nuwan Chamara Chathuranga, Shan Randima Nawarathne, Venuste Maniraguha, Bernadette Gerpacio Sta Cruz, Eunsoo Seo, Jeseok Lee, Jung Min Heo","doi":"10.5713/ab.24.0344","DOIUrl":"10.5713/ab.24.0344","url":null,"abstract":"<p><strong>Objective: </strong>Pre-slaughter transportation adversely impacts the welfare, meat yield, and quality of broilers, yet the effects of different crate types on broiler chickens during winter remain underexplored. The goal of this study was to investigate the effect of plastic and iron crates in transit on meat quality, carcass, and physiological traits of broiler chickens during winter.</p><p><strong>Methods: </strong>A total of 175 (35-day-old) Ross 308 male broilers with an average body weight of about 1,708±33.3 g (mean±standard error of the mean) were picked after 4 hours of feed withdrawal before transport. The control group comprises birds in the farm (n = 15) without transportation at 173 cm2/kg density. The birds were transported into fixed iron (25 birds per crate) and plastic crates (15 birds per crate) with four replicates per crate type at the same 173 cm2/kg densities. The transportation distance was 20 km for 40 min at an average speed of 30-50 km/h early morning at 8:00 am under -1°C and 47% relative humidity.</p><p><strong>Results: </strong>There was no difference (p>0.05) in carcass traits among the treatments. Concerning meat quality, broilers transported in both crate types exhibited lower (p<0.01) a* values compared to the control group. Additionally, the iron crate group demonstrated higher (p<0.05) b* values for the breast meat compared to the other groups. In terms of blood metabolites, the iron crate group had higher (p<0.05) cortisol, glucose, and lactate levels compared to the control group that did not transport.</p><p><strong>Conclusion: </strong>Broilers transported in the iron crates increase stress levels in terms of higher cortisol, glucose, and lactate contents in the blood plasma compared to untransported broilers during the winter. Therefore, employing plastic crates, which induce significantly reduced cortisol and numerically lower glucose levels compared to iron crates, appears more favorable for animal welfare by mitigating stress.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"568-574"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inclusion of imputed genotypes from non-genotyped dairy cattle in a Thai multibreed genomic-polygenic evaluation.","authors":"Danai Jattawa, Thanathip Suwanasopee, Mauricio A Elzo, Skorn Koonawootrittriron","doi":"10.5713/ab.24.0317","DOIUrl":"10.5713/ab.24.0317","url":null,"abstract":"<p><strong>Objective: </strong>This study assessed the impact of incorporating imputed single nucleotide polymorphism (SNP) information from non-genotyped animals on genomic-polygenic evaluations in a Thai multibreed dairy population under various levels of imputation accuracy.</p><p><strong>Methods: </strong>Data encompassed pedigree and phenotypic records for 305-day milk yield (MY), 305-day fat (Fat), and age at first calving (AFC) from 12,859 first-lactation cows, and genotypic records of various densities from 4,364 animals. A set of 64 animals genotyped with GeneSeek Genomic Profiler 80K and with four or more genotyped progenies was defined as target animals to simulate imputation scenarios for non-genotyped individuals. Actual and imputed genotypes were utilized to construct three SNP sets. All SNP Sets contained actual and imputed SNP markers from genotyped animals. SNP Set 1 contained no SNPs from target animals, whereas SNP Set 2 incorporated imputed SNPs from target animals, and SNP Set 3 added actual SNPs from target animals. Genomic-polygenic evaluations were conducted using a 3-trait single-step model that included contemporary group, calving age, and heterozygosity as fixed effects and animal additive genetic and residual as random effects.</p><p><strong>Results: </strong>The imputation accuracy was similar across non-genotyped animals irrespective of the number of genotyped progenies (average: 40.55%; range: 34.68% to 53.82%). Estimates of additive genetic and environmental variances and covariances for MY and AFC varied across SNP sets. SNP Sets 1 and 2 had slightly higher additive genetic and lower environmental variances and covariances than SNP Set 3. Heritabilities and additive genetic, environmental, and phenotypic correlations between MY, Fat, and AFC were similar across all SNP Sets. Spearman rank correlations between genomic-polygenic estimated breeding values from SNP Sets 2 and 3 were high for all traits (0.9990±0.0003).</p><p><strong>Conclusion: </strong>Utilization of phenotypic and pedigree data from imputed non-genotyped animals enhanced the efficiency and cost-effectiveness of the genetic improvement program in the Thai multibreed dairy cattle population.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"419-430"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial morphology and energy metabolism in reprogrammed porcine expanded potential stem cells.","authors":"Yun Ju Lee, Jae Hoon Song, Je Woo Lee, Tae Kyung Hong, Sang Jun Uhm, Kwonho Hong, Jeong Tae Do","doi":"10.5713/ab.24.0521","DOIUrl":"10.5713/ab.24.0521","url":null,"abstract":"<p><strong>Objective: </strong>Expanded potential stem cells (EPSCs) are stem cells that can differentiate into embryonic and extraembryonic lineages, including extraembryonic endoderm and trophoblast lineages. Therefore, EPSCs have great potential in advancing regenerative medicine, elucidating disease mechanisms, and exploring early embryonic development. However, the generation and characterization of EPSCs in pigs have not been thoroughly explored. In this study, we successfully generated porcine EPSCs (pEPSCs).</p><p><strong>Methods: </strong>We reprogrammed porcine fetal fibroblasts (PFFs) using an integration-free method with Sendai virus vectors.</p><p><strong>Results: </strong>The resulting pEPSCs expressed key pluripotency markers and demonstrated the ability to differentiate between embryonic and extraembryonic lineages. Notably, reprogramming into pEPSCs was associated with a transformation of mitochondrial morphology from the elongated form observed in PFFs to a globular shape, reflecting potential alterations in energy metabolism. We observed significant remodeling of mitochondrial morphology and a subsequent shift towards glycolytic energy dependence during the reprogramming of PFFs into pEPSCs.</p><p><strong>Conclusion: </strong>Our findings provide valuable insights into the characteristics of EPSCs in pigs and highlight their potential applications in regenerative medicine, disease modeling, and emerging fields such as cell-based meat production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"444-453"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of processing techniques on energy content and amino acid digestibility in corn germ meal fed to growing pigs.","authors":"Jinbiao Zhao, Zhaoyu Liu, Xiaoming Song, Meiyu Yang, Zhongchao Li, Ling Liu","doi":"10.5713/ab.24.0581","DOIUrl":"https://doi.org/10.5713/ab.24.0581","url":null,"abstract":"<p><strong>Objective: </strong>The study was conducted to determine energy contents, apparent total tract digestibility (ATTD) of nutrient, the apparent (AID) and standardized (SID) ileal digestibility of amino acid (AA) in corn germ meals (CGM) produced by processing technologies of wet milling (CGM-CV1 and CGM-CV2), heating-dried CGM with steep liquor (CGMSL-DH) and dried using indirect heat (CGMSL-IH), corn germ expellers (CGE) and dry-grind processing method (CGM-DG).</p><p><strong>Methods: </strong>In Exp.1, Forty-two crossbred male barrows with an initial body weight (BW) of 51.2 ± 4.5 kg were assigned to 1 of 7 diets in a randomized complete block design. In Exp,2, Seven cannulating barrows with an initial BW of 35.3 ± 1.3 kg were assigned to 7 diets in each period according to a Latin square design. Each diet included 6 replicates.</p><p><strong>Results: </strong>The ATTD of acid detergent fiber (ADF) and gross energy (GE), and SID of most AA in CGMSL-IH was greater (p<0.05) than those in CGM-CV2. The ATTD of organic matter (OM), crude protein (CP) and GE, and SID of most AA in CGMSL-DH was greater (p<0.05) than those in CGM-CV1 for pigs. The ATTD of GE and acid-hydrolyzed ether extract (AEE), contents of digestible (DE) and metabolizable energy (ME), and SID of most AA in CGE were greater (p<0.05) than those in CGM-CV1. The ATTD of OM and GE, and SID of all AA in CGM-DG were greater (p<0.05) than those in CGM-CV1. Compared with the CGM-CV1, CGM-CV2 showed a greater (p<0.05) ATTD of GE and SID of some AA for growing pigs.</p><p><strong>Conclusion: </strong>Different processing technologies lead to large variations on energy contents and amino acids digestibility of corn germ meal for growing pigs. The CGE contains more available energy and CGMSL-DH, CGMSL-IH and CGM-DG provides more ileal digestible AA for growing pigs compared with the CGM-CV1 and CGM-CV2.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of bacteriophage cocktail supplementation in gestation diet on reproductive performance, blood profile, milk composition, and fecal microflora of sows.","authors":"Jinsu Hong, Geon Il Lee, Jae-Cheol Jang, Yoo Yong Kim","doi":"10.5713/ab.24.0796","DOIUrl":"https://doi.org/10.5713/ab.24.0796","url":null,"abstract":"<p><strong>Objective: </strong>The present study aimed to evaluate the effects of bacteriophage cocktail supplementation in gestation sow's diet on gestating sows and their progeny.</p><p><strong>Methods: </strong>A total of 57 F1 multiparous sows (Yorkshire × Landrace) were allotted to one of three treatment groups in a completely randomized design. The sows were fed corn-soybean meal-based diet supplemented with 0, 0.05, or 0.10% of bacteriophage cocktail during the gestation period and they were fed a common diet during the lactation period. Body weight and bacfact thickness were measured throughout the trial along with blood collection for immunoglobulin analysis. Fecal samples were collected on 4, 6, 8, and 10 weeks of gestation period for fecal microflora. Litter performance and milk composition were investigated on 24 hrs postpartum and d 21 of location.</p><p><strong>Results: </strong>Increasing bacteriophage cocktail supplementation levels in gestation diets linearly increased (P < 0.05) feed intake of lactating sows and alive litter weight, and quadratically increased (P < 0.05) litter weight gain from d 0 to 21 of lactation. Dietary treatment did not affect the serum concentrations of immunoglobulin G and immunoglobulin M in the sows. Increasing dietary bacteriophage cocktail levels in gestation diets linearly increased (P < 0.05) the fat content in colostrum. In fecal microflora of sows, the dietary bacteriophage cocktail significantly increased (P < 0.05) the counts of Lactobacillus for d 63 and 105 of the gestation period and decreased the counts of E. coli (P < 0.05) and Salmonella (P < 0.10) for the late gestation period.</p><p><strong>Conclusion: </strong>Supplementation of bacteriophage cocktail at 0.05% in the diet for gestating sows could improve total litter weight and alive litter weight at birth and litter weight gain during the lactation period through a positive change in fecal microflora with an increase of Lactobacillus and decrease of E. coli, and Salmonella.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CIDEB promotes lipid deposition in goat intramuscular adipocytes.","authors":"Zhuohan Huang, Qi Li, Changheng Yang, Changhui Zhang, Lian Huang, Yaqiu Lin, Yong Wang, Hua Xiang, Jiangjiang Zhu","doi":"10.5713/ab.24.0584","DOIUrl":"https://doi.org/10.5713/ab.24.0584","url":null,"abstract":"<p><strong>Objective: </strong>CIDEB (Cell death-inducing DNA fragmentation factor alpha-like effector B), a family member of CIDEs (Cell death-inducing DFF45-like effectors), is well known as a crucial regulator for lipid metabolic signaling pathways in various metabolic tissues and secretory glands. However, its role in regulating intramuscular fat (IMF) deposition in goat remains unclear.</p><p><strong>Methods: </strong>The expression vector pcDNA3.1-CIDEB was constructed and transfected into goat intramuscular preadipocytes; the overexpression and interference efficiency and expression of genes related to lipid metabolism were measured by Real-time PCR; the effect of overexpression of CIDEB and interfering with CIDEB on lipid droplet formation was observed by Oil Red O staining and GPO-Trinder enzymatic reaction. Then RNA-Seq was used to investigate the metabolic pathway of CIDEB affecting adipocyte deposition in goat intramuscular preadipocytes.</p><p><strong>Results: </strong>Overexpression of CIDEB significantly promoted the lipid droplets accumulation and the triglyceride deposition, and significantly upregulated the expression of genes related to lipid metabolism. After overexpression of CIDEB in goat intramuscular preadipocytes, in total 171 differentially expressed genes (DEGs) were found, including 122 up-regulated and 49 down-regulated DEGs, and the top three significantly changed pathways filtered by Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were Cocaine addiction, Amphetamine addiction and Malaria pathways. Conversely, the silencing of CIDEB significantly reduced lipid accumulation in goat intramuscular preadipocytes, meanwhile changed the expression of lipid metabolism genes. For CIDEB silencing, a total of 2140 DEGs were found, including 1252 up-regulated and 888 down-regulated DEGs, and the top three significantly changed pathways filtered by KEGG analysis were Ribosome, Thyroid hormone signaling pathway and Alzheimer disease.</p><p><strong>Conclusion: </strong>The expression of CIDEB can significantly promote lipid deposition of intramuscular adipocytes in goats, and these results provide important data to support further clarifying the mechanism of CIDEB gene on the regulation of intramuscular adipogenesis, and the IMF formation in goat.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular regulatory mechanisms of dietary supplementation with Allium mongolicum Regel powder to improve muscle development and meat quality in Angus calves.","authors":"Wangjing Liu, Chenxu Sun, Huixia Gao, Jianjian He, Aihuan Yu, Yaodi Xie, Haibo Yao, Jiang Hu, Zhaomin Lei","doi":"10.5713/ab.24.0809","DOIUrl":"https://doi.org/10.5713/ab.24.0809","url":null,"abstract":"<p><strong>Objective: </strong>Supplementing animal feed with Allium mongolicum Regel powder (AMRP) additives can promote muscle production and improve meat quality. Here, we explored the effects of dietary AMRP supplementation on the performance, meat quality, and muscle transcriptome profile of Angus calves.</p><p><strong>Methods: </strong>Twelve healthy black Angus calves (female, average body weight = 280.4 ± 15.74 kg, average age = 14 ± 0.6 months) of the same genetic background were randomly assigned to two feed groups: control (CON), basal diet without any supplementation, and AMRP, basal diet supplemented with 20 g of AMRP per calf per day.</p><p><strong>Results: </strong>In general, compared with the control group, dietary AMRP supplementation significantly increased the longissimus thoracis muscle area (p=0.027) and pH24h (p= 0.027) but significantly reduced Warner-Bratzler shear force (p=0.009) and cooking loss (p<0.001). Moreover, 1,284 differentially expressed genes (DEGs) were identified in AMRP-supplemented Angus calves. Pathway analysis revealed that the DEGs were involved in multiple pathways related to muscle development and fat deposition, such as the focal adhesion and MAPK pathways.</p><p><strong>Conclusion: </strong>Dietary supplementation of AMRP improved muscle growth and development in Angus beef cattle, as well as significantly modulated meat quality, and this modulation may affect signaling pathways by influencing the expression of key genes. Our results provide new insights into the development of the meat industry and, most importantly, analyze the mechanism by which AMRP regulates muscle development and improves meat quality at the molecular level.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}