Animal Bioscience最新文献

{"title":"Effects of dietary rumen undegradable protein:rumen degradable protein ratio on nitrogen metabolism in Hanwoo steers.","authors":"Sang Yeob Kim, Rajaraman Bharanidharan, Seyun Im, Kyoung Hoon Kim, Joonpyo Oh, Hyun Jin Kim, Jaesung Lee, Kamburawala Kankanamge Tharindu Namal Ranaweera, Jin Woo Jeong, Jun Seok Oh, Sang Hyun Lee, Myunggi Baik","doi":"10.5713/ab.24.0592","DOIUrl":"10.5713/ab.24.0592","url":null,"abstract":"<p><strong>Objective: </strong>We investigated the effects of dietary rumen undegradable protein (RUP): rumen degradable protein (RDP) ratio on growth performance, nitrogen (N) metabolism, and rumen and blood parameters in Hanwoo (Korean cattle) steers.</p><p><strong>Methods: </strong>Eight Hanwoo steers (average body weight, 393 kg) were allocated to two groups and fed with a high RUP:RDP ratio (46.9:53.1 based on crude protein) or a low RUP:RDP ratio (30.6:69.4) concentrate with iso-crude protein content in a 2×2 Latin square design in two successive periods.</p><p><strong>Results: </strong>The high RUP:RDP group had higher (p<0.01) average daily gain, and lower (p<0.05) ruminal ammonia and plasma urea N concentrations than the low RUP:RDP group. The high RUP:RDP group had lower (p<0.05) urinary N excretion and urinary N per N intake and higher (p<0.1) tendency of retained N than the low RUP:RDP group. The high RUP:RDP group had higher (p<0.1) tendency of N utilization efficiency (retained N per N intake: 28.7% vs. 25.5%) than the low RUP:RDP group. The high RUP:RDP group had a lower (p<0.1) tendency of urinary N per total N excretion, but a higher tendency of fecal N per total N excretion.</p><p><strong>Conclusion: </strong>A high ratio of dietary RUP:RDP may improve N utilization efficiency by reducing urinary N excretion, which may be beneficial for the environment through reducing atmospheric ammonia emissions.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1182-1193"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-21-5p enhances differentiation and mitigates oleic acid-induced lipid droplet accumulation in C2C12 myoblasts by targeting FBXO11.","authors":"Yi Li, Jiayuan Fang, Yingying Jiao, Qinchuan Lv, Xingyu Xiao, Shuo Zheng, Xi Chen, Jie Song, Xunming Zhang, Libo Zhang, Ze Ma, Changhong Li, Linlin Hao","doi":"10.5713/ab.24.0665","DOIUrl":"10.5713/ab.24.0665","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the role of miRNA 21-5p in regulating the differentiation of C2C12 myoblasts and intramuscular lipid droplets accumulation in myotubes.</p><p><strong>Methods: </strong>The role of miR-21-5p in the proliferation and differentiation of myofibroblasts and intracellular lipid accumulation was analyzed using bioinformatics, CCK-8 assay, quantitative real-time polymerase chain reaction, immunoblotting, immunofluorescence staining, and Oil Red O staining.</p><p><strong>Results: </strong>The analysis of porcine BodyMap transcriptome data revealed differential expression of miRNA 21-5p in skeletal muscle and adipose tissue. Bioinformatics analysis combined with a dual-luciferase reporter assay demonstrated that FBXO11 serves as a direct target of miR-21-5p. Transfection experiments involving a miR-21-5p mimic, miR-21-5p inhibitor, and si-FBXO11 in C2C12 cells showed that overexpression of miR-21-5p or silencing of FBXO11 significantly enhanced the proliferation of C2C12 cells, upregulated myogenesis-related factors, and promoted myotube formation. Furthermore, oleic acid-induced lipid accumulation in myotubes was suppressed, accompanied by reduced expression of adipogenesis-related genes. Conversely, inhibition of miR-21-5p expression produced opposite effects.</p><p><strong>Conclusion: </strong>These findings indicate that miR-21-5p promotes proliferation and differentiation while inhibiting intramyocellular lipid deposition by targeting the 3'-untranslated region of FBXO11 in myogenic cell. The results suggest that miR-21-5p could serve as a potential miRNA biomarker for regulating intramuscular adipogenesis.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1279-1290"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of individual and combined doses of added nitrite and nitrate on the quality of dry-fermented sausage.","authors":"Suk Nam Kang, Donggyun Yim","doi":"10.5713/ab.24.0561","DOIUrl":"10.5713/ab.24.0561","url":null,"abstract":"<p><strong>Objective: </strong>We aimed to investigate the impacts of nitrate and nitrite on the salami fermentation process.</p><p><strong>Methods: </strong>Experimental groups of dry-fermented sausage were prepared with 100 ppm nitrite (T1), 150 ppm nitrite (T2), and 100 ppm nitrite+50 ppm nitrate (T3). Salami quality was evaluated after a 30-day fermentation-drying process.</p><p><strong>Results: </strong>There were no significant differences in residual nitrite levels between T1 and T2, while the T3 samples exhibited the highest residual nitrite. T2 (150 ppm nitrite) showed a significantly higher pH value compared to T1 (100 ppm nitrite). Water activity was lowest in the T2 sample. T1 had a higher thiobarbituric acid reactive substances value than other treatments. In the color analysis, the L* values of T1 and T2 were significantly higher than those of T3, whereas the a* values were significantly higher in T3. In the texture analysis, T3 exhibited a higher shear force than T1 and T2. In the microbial analysis, adding 50 and 100 ppm of nitrite did not reduce the total plate count (TPC) and lactic acid bacteria count. However, T3 had a lower TPC than T1 and T2.</p><p><strong>Conclusion: </strong>The results suggest that different levels of nitrite/nitrate affect salami quality. This study is a valuable resource for understanding how using nitrite and nitrate alone or in combination affects quality in salami production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1272-1278"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of high-protein concentrates with different rumen undegradable-to-degradable protein ratios on performance and carcass traits in 26-month-slaughtered Hanwoo steers.","authors":"Woo Hyeong Hong, Rajaraman Bharanidharan, Tae Hoon Kim, Jun Suk Byun, Geum Hwi Bang, Krishnaraj Thirugnanasambantham, Joonpyo Oh, Ridha Ibidhi, Sun Sik Jang, Kyoung Hoon Kim","doi":"10.5713/ab.24.0728","DOIUrl":"10.5713/ab.24.0728","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the effects of high-protein concentrates with two different rumen undegradable protein (RUP)-to-degradable protein ratios on the performance and carcass characteristics of Hanwoo steers reared for 26 months.</p><p><strong>Methods: </strong>Thirty-two Hanwoo steers (217±13 kg) were randomly allocated to eight pens (four animals/pen) and assigned to one of two concentrate treatments (four pens/treatment), low-RUP concentrate (LRUP; RUP ratio of 37:63) or high-RUP concentrate (HRUP; RUP ratio of 47:53), with a dietary crude protein (CP) content of 20% dry matter (DM) during the growing stage (8 to 17 months) and 18% DM during the fattening stage (18 to 26 months).</p><p><strong>Results: </strong>Increasing the RUP ratio in the concentrate reduced (p = 0.062) rumen ammonia (NH3-N) levels during the growing stage. A decrease (p<0.05) in the molar proportions of acetate and valerate, and an increase (p = 0.097) in the proportions of iso-valerate, was observed in the HRUP group. Significant improvements (p<0.05) in the average daily gain and the feed conversion ratio were observed in the HRUP group during the fattening stage. Although there were no significant group differences in carcass yield or characteristics in steers slaughtered at 26 months, a trend (p = 0.081) toward an increased rib-eye area was observed in the HRUP group. Relative mRNA expression profiling revealed higher lipid biosynthesis and lipolysis in the HRUP group at slaughter. However, no effects on intramuscular fat content was detected.</p><p><strong>Conclusion: </strong>Raising the RUP level improved performance and intramuscular fat metabolism in Hanwoo steers. The final body weight of the steers in the HRUP group was comparable to that of Hanwoo steers raised under the conventional 30-month fattening period. Overall, feeding a high-CP concentrate with a higher RUP proportion (47% CP) may be a beneficial strategy to shorten the feeding period.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1206-1218"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leucyl-tRNA synthetase regulates casein synthesis in dairy cows via the mTORC1-LAT1 pathway.","authors":"Yongding Ke, Ximeng Du, Binglan Chen, Xi Chen, Chengchuang Song, Xingtang Fang, Yanhong Wang, Chunlei Zhang","doi":"10.5713/ab.24.0711","DOIUrl":"10.5713/ab.24.0711","url":null,"abstract":"<p><strong>Objective: </strong>Leucyl-tRNA synthetase (LARS) is an essential multifunctional enzyme in mammals, pivotal in maintaining cellular protein and amino acid balance. It facilitates tRNA aminoacylation, initiating intracellular protein synthesis, and serves as an intracellular leucine sensor. The sensor function enables LARS to activate the mTORC1 pathway via Rag GTPase binding, playing a critical role in the regulation of protein synthesis. Despite its significance, the precise mechanisms of these functions are yet to be fully delineated. This study examines LARS and its role in modulating milk protein synthesis.</p><p><strong>Methods: </strong>This study utilizes stable bovine mammary epithelial cell lines, LARS overexpression and LARS knockdown, validated by using Cell Counting Kit-8, Click-iT EdU, Western blot, real-time quantitative polymerase chain reaction, and immunoconfocal techniques.</p><p><strong>Results: </strong>Our findings show that LARS overexpression in bovine mammary epithelial cells (MAC-T) enhances cell proliferation and resultes intracellular leucine levels, thereby increasing casein production through the mTORC1 pathway. LARS enhances casein expression via the mechanistic Target of Rapamycin Complex 1, L-type Amino Transporters 1 (mTORC1-LAT1) pathway. This interaction is supported by a positive feedback mechanism from LAT1, enhancing the activation of the mTORC1 pathway. Additionally, LARS overexpression leads to increased LAT1 expression, improved LAT1 stability, and augmented its localization at the membrane. Our research indicates that LARS's enhancement of LAT1 expression is contingent on its dual roles in translation and leucine sensing, whereas its impact on LAT1 localization is exclusively dependent on its leucine sensing function.</p><p><strong>Conclusion: </strong>LARS regulates LAT1 expression and membrane positioning through the mTORC1 pathway by detecting intracellular leucine levels, thereby influencing casein synthesis. These insights lay a theoretical groundwork for enhancing milk protein production and offer novel strategies for improving the quality of dairy products.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1259-1271"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rumen by-pass soybean meal reduced ruminal ammonia but did not improve growth performance and nitrogen utilization in growing Hanwoo heifers.","authors":"Joonpyo Oh, Dae Kyum Yoo, Si Woo Jeong, Hyun Wook Shin, Jong Min Kim, Ja-Kyeom Seo","doi":"10.5713/ab.24.0860","DOIUrl":"10.5713/ab.24.0860","url":null,"abstract":"<p><strong>Objective: </strong>The effects of heat-treated soybean meal (SBM) and citric acid (HCSBM) on the growth performance, rumen fermentation, blood metabolites, nutrient digestibility, and nitrogen (N) utilization were evaluated in growing Hanwoo heifers.</p><p><strong>Methods: </strong>Eight growing Hanwoo heifers (initial body weight: 228.5±11.3 kg; age: 9.3±0.3 months) were allocated to a crossover design with two dietary treatments: a control diet containing untreated SBM (Control) and a diet containing HCSBM. There were two 28-day phases in the trial, each containing 14-day measurements and an adaptation period. Growth performance, rumen fermentation parameters, blood metabolites, hematological parameters, apparent digestibility, energy, and N utilization were measured.</p><p><strong>Results: </strong>No significant differences were observed in the growth performance or energy utilization between the diets. The digestibility of dry matter and crude protein (CP) did not differ between the two diets. HCSBM supplementation increased neutral detergent fiber digestibility (p = 0.0874) and acetate molar proportions (p = 0.0748). The HCSBM diet resulted in lower ruminal ammonia nitrogen concentrations and iso-valerate proportions (p<0.05), indicating reduced ruminal protein degradation. Blood metabolites related to protein metabolism showed no significant differences between treatments. The control group exhibited higher red blood cell counts and hemoglobin and hematocrit levels (p<0.05). N excretion or retention did not significantly differ between the dietary groups.</p><p><strong>Conclusion: </strong>Despite enhanced protection against ruminal protein degradation, HCSBM supplementation did not improve N utilization efficiency or growth performance in growing Hanwoo heifers, possibly because of a sufficient N supply from the high CP content in both diets.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":"38 6","pages":"1219-1227"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Brix values for estimating nitrogen and gross energy concentrations of pig urine: a technical note.","authors":"Jeonghyeon Son, Hyunseok Do, Beob Gyun Kim","doi":"10.5713/ab.24.0684","DOIUrl":"10.5713/ab.24.0684","url":null,"abstract":"<p><p>The present study aimed to develop equations for estimating the dry matter (DM), nitrogen (N), and gross energy (GE) concentrations in pig urine using Brix values. A total of 96 urine samples were obtained from pigs of 6.0 to 62.2 kg initial body weight fed 12 diets in three experiments. The animals were fed a corn-soybean meal-based diet or a corn diet. The N concentrations in the urine samples ranged from 0.12% to 2.06% and the GE concentrations ranged from 2 to 187 kcal/kg. The urine Brix values were positively correlated with urinary DM, N, and GE concentrations (r = 0.94, 0.87, and 0.93, respectively; p<0.001). The models for estimating urinary DM, N, and GE concentrations were: urinary DM (%) = 0.590×Brix+0.315 (r2 = 0.91; p<0.001); urinary N (%) = 0.0798×Brix+0.148 (r2 = 0.84; p<0.001); urinary GE (kcal/kg) = 11.96×Brix+2.33 (r2 = 0.87; p<0.001), where the Brix value of urine is expressed as %. Overall, the Brix values correlated positively with the DM, N, and GE concentrations in pig urine and can thus be used for estimating the concentrations of these urinary components.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1242-1246"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fisetin alleviates oxidative stress and promotes porcine early embryonic development via activation of the NRF2-ARE signalling pathway.","authors":"Hua-Kai Wei, Jia-Jia Qi, Yan-Qiu Wang, He-Xuan Qu, Chen-Xuan Yan, Tian-Tian Li, Yu Wang, Hao Sun, Bo-Xing Sun, Shuang Liang","doi":"10.5713/ab.24.0691","DOIUrl":"10.5713/ab.24.0691","url":null,"abstract":"<p><strong>Objective: </strong>We improved the developmental capacity of porcine early embryos via supplementation with fisetin during in vitro culture (IVC). In addition, we investigated the antioxidant mechanism of fisetin via activation of the NRF2-ARE signalling pathway in porcine early embryos.</p><p><strong>Methods: </strong>Fisetin (0, 1, 2.5 and 5 μM) was supplemented during IVC to observe its effects on the developmental ability of porcine parthenogenetic activation (PA), in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) embryos. The effects of fisetin supplementation on the antioxidant capacity, mitochondrial function, cell proliferation and apoptosis levels of porcine PA embryos were detected via fluorescence staining, and the expression levels of genes related to apoptosis, pluripotency and the NRF2 pathway were also examined.</p><p><strong>Results: </strong>Compared with the control, 1 μM fisetin during IVC increased the developmental ability of porcine PA, IVF and SCNT embryos. Additionally, fisetin significantly decreased reactive oxygen species (ROS) and apoptosis levels; increased pluripotency during embryonic development, cell proliferation and glutathione levels; and improved mitochondrial function in PA embryos. Moreover, the levels of Kelch-like ECH-associated protein 1 (KEAP1) significantly decreased, and the levels of NFE2-like bZIP transcription factor 2 (NRF2) and its downstream antioxidant enzymes significantly increased after fisetin supplementation.</p><p><strong>Conclusion: </strong>Our data reveal that fisetin protects porcine early embryos from oxidative stress during IVC by activating the NRF2-ARE signalling pathway, thereby improving the success of in vitro embryo production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1160-1174"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of yam bean pulp on growth performance, gut morphology, digestive development, and digestibility in broilers raised in hot environments.","authors":"Kedsirin Sakwiwatkul, Wannisa Ojan, Purinut Treehera, Jessada Pidtathasa, Theeranon Pomwong, Anut Chantiratikul, Siriporn Lawan, Manisa Sangkaew","doi":"10.5713/ab.25.0057","DOIUrl":"https://doi.org/10.5713/ab.25.0057","url":null,"abstract":"<p><strong>Objective: </strong>Rearing broilers in high-temperature environments can impair growth performance, affecting the economic efficiency of broiler production. Dietary fiber sources like yam bean pulp (YBP) may mitigate these effects by promoting digestive organ development and gut integrity, enhancing nutrient absorption and growth. This study aimed to evaluate the impact of YBP inclusion on digestive development, gut morphology, nutrient digestibility, serum lipid profiles, and growth performance in broilers raised under high environmental temperatures.</p><p><strong>Methods: </strong>Since YBP has not previously been used in poultry diets, its optimal inclusion level was determined using in vitro digestibility measurements, which identified 4% YBP as the optimal initial inclusion level for further in vivo study. Subsequently, the in vivo study was conducted with 200 one-day-old ROSS broiler chicks, randomly assigned to four dietary treatments (0%, 4%, 8%, and 12% YBP; 5 replicates of 10 birds each), and housed in an open system at an average temperature of 32.1 ± 3.7°C. Digestive traits, nutrient digestibility, serum lipid profiles, and growth performance were assessed over 21 days.</p><p><strong>Results: </strong>Results showed that YBP inclusion (up to 12%) improved gut morphology by increasing villi height and the villi height-to-crypt depth ratio in the duodenum and ileum, alongside dose-dependent improvements in dry matter digestibility. However, crude protein digestibility declined when YBP levels exceeded 8%. Despite these benefits, YBP inclusion did not alleviate heat stress effects on growth performance, digestive organ development, or serum lipid profiles, even at the highest inclusion level.</p><p><strong>Conclusion: </strong>Dietary YBP can enhance gut morphology and nutrient digestibility in broilers from day 1 to 21, with optimal inclusion levels not exceeding 8%. However, its use does not mitigate the negative effects of heat stress on broiler growth in high-temperature environments.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phuphan Chicken Breeds: Classification as Varieties or Distinct Breeds with Three Derivative Groups using Microsatellite Genotyping.","authors":"Ekerette Ekerette, Nivit Tanglertpaibul, Trifan Budi, Wisut Auekingpetch, Chien Phuoc Tran Nguyen, Worapong Singchat, Wongsathit Wongloet, Nichakorn Kumnan, Piangjai Chalermwong, Anh Huynh Luu, Thitipong Panthum, Aingorn Chaiyes, Kanithaporn Vangnai, Chotika Yokthongwattana, Chomdao Sinthuvanich, Narongrit Muangmai, Prateep Duengkae, Kornsorn Srikulnath","doi":"10.5713/ab.24.0579","DOIUrl":"https://doi.org/10.5713/ab.24.0579","url":null,"abstract":"<p><strong>Objective: </strong>Indigenous and local breeds, such as Phuphan chickens, are vital owing to their adaptability and nutritional content. However, precise origin, historical records, and genetic diversity of Phuphan chickens remain unclear. Thus, this study aimed to assess origin and genetic diversity of four Phuphan chicken groups from the Phuphan Royal Development Study Centre.</p><p><strong>Methods: </strong>This study assess four Phuphan chicken groups: Phuphan black 1 (SK-B1), Phuphan black 2 (KU-BM/F), Phuphan white (KU-WM/F), and Phuphan color (KU-VM/F) using 28 microsatellite markers and compared with those of other Thai chicken breeds under \"The Siam Chicken Bioresource Project\" database.</p><p><strong>Results: </strong>The results highlighted significant genetic diversity among these groups (mean expected heterozygosity (He)=0.623 ± 0.014; Allelic richness (AR)=4.594 ± 0.124), indicating effective management through the breeding program of the Phuphan Royal Development Study Centre. Distinct gene pools were identified through population structure analyses, emphasizing that SK-B1 is genetically unique compared to the other three groups. Bayesian inference confirmed historical genetic exchanges, primarily between KU-BM/F, KU-WM/F, and KU-VM/F, with minimal exchanges involving SK-B1. This suggests that the Phuphan chicken groups share a common lineage, differentiated mainly by plumage color, through remnant selection processes. Microsatellite markers pinpointed the loci LEI0234, MCW206, MCW0016, MCW0222, MCW0098, MCW0165, and ADL0278 as potentially under directional selection and linked to plumage color variation among the Phuphan chicken groups. Comparative evaluations with other Thai indigenous and local chickens and red junglefowl revealed a closer affinity of SK-B1 to existing Thai chicken breeds, suggesting that it may represent a variant of Thai chicken breeds. Alternatively, KU-BM/F, KU-WM/F, and KU-VM/F, which maintained similar outward appearances, may constitute a new Phuphan chicken breed.</p><p><strong>Conclusion: </strong>These findings could improve understanding on genetic architecture of Phuphan chicken groups and could enhance Thailand's economic growth while conserving the genetic diversity of these indigenous chickens.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}