Animal Bioscience最新文献

{"title":"A practical approach for the stable isolation and cultivation of chicken gonadal primordial germ cells with mitotically inactivated STO feeder cells.","authors":"Hyeon Yang, Bo Ram Lee, Jae-Yong Lee, Keon Bong Oh, Poongyeon Lee, Seunghoon Lee, Yong Jin Jo, Haesun Lee, Seokho Kim, Jingu No, Jae Yong Han, Sung June Byun","doi":"10.5713/ab.25.0192","DOIUrl":"https://doi.org/10.5713/ab.25.0192","url":null,"abstract":"<p><strong>Objective: </strong>Establishing chicken primordial germ cells (PGCs) in vitro is critical for producing genetically modified (GM) chickens. Efficient and reliable isolation and cultivation of PGCs remain significant challenges in advancing avian genetic modifications. To address these challenges, we employed a streamlined and practical approach for the efficient isolation and stable cultivation of chicken gonadal PGCs.</p><p><strong>Methods: </strong>Chicken gonadal PGCs were isolated from embryonic gonads, surgically removed and dissociated using trypsin. The PGCs were isolated by exploiting differential adhesion properties, allowing fibroblasts to attach while PGCs remained suspended. Cultivation was performed with mitotically inactivated STO feeder cells under optimized culture conditions.</p><p><strong>Results: </strong>PGCs proliferated robustly, reaching over 105 cells within one month, which is comparable to previously reported methods. Characterization assays confirmed the expression of PGC-specific markers, including SSEA-1 and DAZL, along with pluripotency-related genes such as OCT4 and NANOG. Additionally, labeled PGCs successfully migrated to recipient embryonic gonads, where they were identified through fluorescence analysis.</p><p><strong>Conclusion: </strong>This study highlights the effectiveness of the proposed method in avian germ cell research, contributing to progress in the production of germline chimeric and GM chickens.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Grazing Practice Affect the Growth Performance, Meat Quality and Nutritional Composition and Fecal Microbiota of Fattening Yaks.","authors":"KeWei Hu, Qi Wu, Tianxiang Chen, Jiakun Wang, Zhixianghi Zhu, Yulei Shen, Chong Wang, Xiaoshi Wei","doi":"10.5713/ab.25.0052","DOIUrl":"https://doi.org/10.5713/ab.25.0052","url":null,"abstract":"<p><strong>Objective: </strong>Livestock grazing is the primary practice in alpine meadows, which is closely related to animal performance and ecosystem functions. This study aimed to evaluate the effects of grazing practice on the growth performance, meat nutritional composition and shelf life, and fecal nutrient and microbiota of yaks.</p><p><strong>Methods: </strong>Twenty-four male yaks (217.62 ± 5.74 kg) were randomly divided into 2 groups for a 60 d fattening experiment: grazing (G) group and grazing and supplementary feeding (GS) group. The yaks in the G group were grazed only on natural pastures, without any supplements. The yaks in the GS group were not only grazed on natural pastures, also supplemented with the concentrate mix based on the body weight after grazing.</p><p><strong>Results: </strong>Supplementary feeding concentrate mix after grazing significantly increased the body weight (p < 0.01) and average daily gain (p < 0.01) of yaks. The results indicated that supplementary feeding reduced meat shear force (p = 0.04), increased the a* value (p < 0.01), the b* value (p = 0.04) and the ether extract content (p = 0.03), and extended the shelf life by 3.4 h. The total amino acid content increased (p < 0.01) and promoted the deposition of monounsaturated fatty acids (p < 0.01) and polyunsaturated fatty acids (p < 0.01). The output concentration of nitrogen in feces was increased (p = 0.04), and the 16S rRNA sequencing results showed that grazing with supplementary feeding significantly increased the relative abundance of key genera, including Alistipes, UCG-009, Tuzzerella, Family_XIII_UCG-001, and Erysipelatoclostridium, which are associated with nutrient absorption, fiber degradation, and metabolism.</p><p><strong>Conclusion: </strong>Post-grazing concentrate mix supplementation improved yak growth, meat quality, and shelf life, likely via enhanced amino acid and fatty acid deposition, nitrogen retention, and gut microbial shifts-offering new insights into nutrient metabolism and feeding strategies for high-altitude livestock.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GATAD2B regulates spindle assembly by affecting protein deacetylation during oocyte meiotic maturation.","authors":"Qian Xu, Lina Yu, Yuling Lin, Aolei Guo, Yang Zhang, Zhe Zhang, Guijun Yan, Haixiang Sun, Guangyi Cao","doi":"10.5713/ab.25.0013","DOIUrl":"https://doi.org/10.5713/ab.25.0013","url":null,"abstract":"<p><strong>Objective: </strong>Oocyte quality is critical for the stable transmission of genetic information and affects early embryonic development. But the precise mechanisms governing oocyte meiotic progression remains largely unclear. Transcription regulation through chromatin compaction and decompaction is regulated through various chromatin-remodeling complexes such as nucleosome remodeling and histone deacetylation (NuRD) complex. GATAD2B is known to be a component of the NuRD complex but whether GATAD2B regulates chromatin modification in mouse oocyte meiosis remains unclear. We hope to explore the role of GATAD2B in mouse oocyte meiosis.</p><p><strong>Methods: </strong>In this study, we initially utilized western blot and immunofluorescence to delineate the expression and subcellular localization of GATAD2B during oocyte meiotic maturation. To further elucidate the role of GATAD2B in regulating oocyte meiotic division, we employed the method of microinjection of Gatad2b-specific siRNA to knock down the protein expression of GATAD2B. Subsequently, dynamic changes in oocyte meiotic division were captured in real-time using live-cell imaging with Geri. Additionally, spindle staining, DNA staining, spread analysis, and reanalysis of RNA-seq data were performed to investigate the mechanisms through which GATAD2B regulates oocyte meiotic division.</p><p><strong>Results: </strong>GATAD2B was stably expressed during oocyte meiosis and was significantly increased during the MII stage. To further explore the effect of GATAD2B on oocyte meiotic maturation, we observed increased abnormal spindle, severe chromosome misalignment and MI block in GATAD2B knocked-down oocytes. Interestingly, the distribution of microtubule organizing center was abnormal and aneuploidy was significantly increased in Gatad2b-KD oocytes. In addition, some deacetylation-related genes were significantly downregulated and acetylated proteins accumulated abnormally in Gatad2b-KD oocytes.</p><p><strong>Conclusion: </strong>These findings implicate GATAD2B as a novel regulator of histone deacetylation during oocyte maturation and provide evidence that such deacetylation is required for proper spindle assembly.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacillus rhamnosus GR-1 alleviates Escherichia coli-triggered bovine endometrial epithelial cells damage via the ROS-mitochondrial pathway.","authors":"Xiaowei Feng, Yan Li, Xiangfu Wen, Mingque Feng, Tianxiong Jin, Muhammad Shahid, Yan Sun, Jiawei Liu, Bei Liu, Jia Cheng, Mingchao Liu","doi":"10.5713/ab.25.0031","DOIUrl":"https://doi.org/10.5713/ab.25.0031","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to evaluate the function of the reactive oxygen species (ROS)-mitochondrial pathway in attenuating Escherichia coli (E. coli) induced apoptosis in bovine endometrial epithelial cells (BENDs) by Lactobacillus rhamnosus (L. rhamnosus) GR-1.</p><p><strong>Methods: </strong>The BENDs were exposed to preincubation with and without L. rhamnosus GR-1 for 3 hours (h) and they were later subject to E. coli for 6 h. The release of lactate dehydrogenase (LDH), the expression of oxidative factors, the expression of mitochondrial membrane potential (MMP), apoptotic rate and apoptosis-associated protein expression were observed. Then, ROS expression, MMP level and cell rate apoptosis rate were further detected after the intervention of antioxidant n-acetyl-l-cysteine (NAC).</p><p><strong>Results: </strong>L. rhamnosus GR-1 was capable of obviously alleviating the content of LDH, ROS expression, the apoptotic rate and MDA concentrations in BENDs induced by E. coli (p<0.01). In addition, L. rhamnosus GR-1 could notably promote the levels of antioxidant factors (SOD, GSH, T-AOC) (p<0.01), inhibit the depolarization of MMP (p<0.01), and levels of apoptosis-related factors (Bax, Caspase-3 and Cyt-c), and promote anti-apoptosis factor (Bcl-2) (p<0.01). Moreover, after the intervention of antioxidant NAC, ROS expression, MMP depolarization, and apoptotic rate induced by E. coli in BENDs were decreased (p<0.01).</p><p><strong>Conclusion: </strong>In E. coli-induced apoptosis in BENDs, therapeutic strategies aimed at down-regulating ROS and MDA and up-regulating antioxidant factors may maintain mitochondrial function and provide theoretical support for the use of probiotics in bovine endometritis.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a multi-strain probiotic on productive traits, antioxidant defence, caecal microbiota and short‑chain fatty acid profile, and intestinal histomorphology in rabbits.","authors":"Vincenzo Tufarelli, Caterina Losacco, Gianluca Pugliese, Alessandra Tateo, Michele Schiavitto, Fabrizio Iarussi, Vito Laudadio, Letizia Passantino","doi":"10.5713/ab.24.0716","DOIUrl":"10.5713/ab.24.0716","url":null,"abstract":"<p><strong>Objective: </strong>This paper aimed to evaluate the effects of a multi-strain probiotic (MS-Prob) as natural feed additives on the productive performance, blood parameters, antioxidant defence, caecal short-chain fatty acid profile as well as the effectiveness on the intestinal morphology and on the equilibrium modification of caecal microbiota of growing rabbits.</p><p><strong>Methods: </strong>Eighty-six-week-old Italian White rabbits were assigned into two dietary groups: the control group was fed without any additive, while the test group received a diet supplemented with a MS-Prob (Slab51) at a dosage of 250 mg/kg diet. The feeding trial lasted up to 12 weeks of age.</p><p><strong>Results: </strong>Rabbits MS-Prob diet had significantly higher daily and final body weight recording also the best of feed efficiency compared to control group. Rabbits in MS-Prob group showed improved slaughter weight and carcass dressing yield. No significant effect was found on meat protein, lipids and ash contents. Serum total cholesterol, low-density lipoprotein, high-density lipoprotein and triglycerides decreased significantly in rabbits fed the test-diet. In rabbits fed MS-Prob, the activity of glutathione peroxidase, glutathione S-transferase, catalase and superoxide dismutase increased significantly, whereas the level of thiobarbituric acid-reactive substance decreased significantly. Caecal pH, ammonia-N and total volatile fatty acids (VFAs) were not significantly influenced by dietary treatments. Conversely, VFAs molar proportions were statistically affected by diets, with higher acetic and butyric acid concentrations in caecum of rabbits fed MS-Prob. Feeding of MS-Prob reduced harmful bacterial growth (Escherichia coli, Bacillus spp., Clostridium spp.) while promoting beneficial bacteria (Lactobacillus spp., Bacteroides spp.). Moreover, MS-Prob positively influences rabbit gut histomorphology, showing higher villus height, villus width, and crypt depth.</p><p><strong>Conclusion: </strong>This study indicated that MS-Prob (Slab51) supplementation stimulated the performance of growing rabbits and positively affected blood serum parameters, intestinal morphology, and caecal environment and microbiota.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1247-1258"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of genetic diversity and inbreeding in the Okinawa indigenous Agu pig through pedigree analysis.","authors":"Shihei Touma, Kyota Fusho, Aisaku Arakawa","doi":"10.5713/ab.24.0646","DOIUrl":"10.5713/ab.24.0646","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to clarify the changes in genetic diversity and inbreeding over the years as well as to identify the causes of genetic diversity loss using the pedigree of Okinawa indigenous Agu pigs.</p><p><strong>Methods: </strong>The pedigree of the Agu population consisted of 1,997 animals from 1997 to 2020. The equivalent complete generations (ECG), inbreeding coefficient (F), and effective population size (Ne) were computed using ENDOG v4.8. Genetic diversity parameters, including founders (f), the effective number of founders (fe), the effective number of ancestors (fa), and the founder genome equivalent (fge), were derived from the probability of gene origin. The study also investigated changes in genetic diversity indices over time.</p><p><strong>Results: </strong>ECG increased from 0.1 in 2003 to 3.87 by 2020, indicating an improved pedigree depth. The F has been on a downward trend since it peaked at 10.5% in 2015. However, the Ne in the reference population was 14.6, which is below the recommended levels. The f, fe, fa and fge of the reference population were 86, 26, 19, and 11.5, respectively. These values decrease in the order of f>fe>fa>fge. This indicates that all factors of genetic diversity loss played a role, including the unbalanced contributions of founders, bottlenecks, and random gene losses. Although in recent years efforts have been made to maintain genetic diversity and reduce inbreeding, the Agu pig population exhibited a 5% decrease in genetic diversity over the past 18 years, with both unequal founder contributions and genetic drift identified as significant contributing factors.</p><p><strong>Conclusion: </strong>This study reveals that more favorable breeding strategies (such as optimal contribution and population size expansion) are required to maintain genetic diversity and increase the Ne of the Agu pig population.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1105-1115"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of hormonal treatments on progesterone levels to enhance embryo survival and kidding rates in goats.","authors":"Manita Wittayarat, Navapol Kupthammasan, Hakim Jehdo, Ratree Jintana, Sopita Suttikrai, Niraporn Tongkumtae, Nantarat Chutijiratthitkan, Pokchon Khirilak, Sirirat Norsoongnern, Supitcha Kaewma, Chaiyawan Wattanachant, Saritvich Panyaboriban","doi":"10.5713/ab.24.0578","DOIUrl":"10.5713/ab.24.0578","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the efficiency of different estrous synchronization programs and hormonal treatments in improving reproductive outcomes in goats. Conducted on a commercial farm in southern Thailand, the study used multiparous Shami and Anglo-Nubian breed goats.</p><p><strong>Methods: </strong>In experiment 1, goats were randomly allocated to two estrous synchronization treatments: 11-day (CI11D) and 13-day (CI13D) intravaginal progesterone implants, followed by artificial insemination (AI) with frozen-thawed semen. Various hormonal treatments (human chorionic gonadotropin [hCG], gonadotropin-releasing hormone [GnRH] analogue, progestogen) were administered on day 5 post-AI to elevate progesterone levels. Experiment 2 assessed embryo survival after transferring early- and late-stage embryos, using GnRH analogue to increase progesterone levels in the recipient goats.</p><p><strong>Results: </strong>Results showed that GnRH analogue significantly increased pregnancy rates, kidding rates, and the number of kids born in the CI13D group. Progesterone levels were higher in treated groups, particularly with GnRH analogue, though estradiol levels varied between synchronization protocols. Late-stage embryo transfers significantly improved pregnancy rates and reduced embryonic loss compared to early-stage transfers. GnRH analogue supplementation reduced early embryonic mortality, enhancing embryo survival and overall reproductive performance.</p><p><strong>Conclusion: </strong>This study demonstrates the efficacy of tailored estrous synchronization and hormonal treatments in optimizing goat reproductive outcomes, with significant implications for improving fertility management in commercial goat farming. Although no significant differences were observed in progesterone levels, the administration of GnRH analogue reduced early embryonic mortality and improved reproductive outcomes, demonstrating its potential to enhance embryo survival and reproductive performance in goats.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1140-1149"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Milk profiles of selenoamino acids, selenoenzymes, and selenoproteins of peripartal dairy cows fed with different selenium sources.","authors":"Xiaoyu Ji, Xuejuan Deng, Ning Liu, Jianping Wang","doi":"10.5713/ab.24.0571","DOIUrl":"10.5713/ab.24.0571","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the effect of dietary nanoselenium (nanoSe) and sodium selenite (SS) on the concentrations of selenoamino acids, the activities of selenoenzymes, and the mRNA expressions of selenoproteins in the milk of peripartal dairy cows.</p><p><strong>Methods: </strong>Three diets included a control (basal diet) with background selenium at 0.06 mg/kg and treatments with either SS or nanoSe added at the same selenium concentration of 1.00 mg/kg of diet. A total of 45 dairy cows were randomly allocated to three groups. The feeding trial lasted for 42 days from prenatal 21 days to postnatal 21 days.</p><p><strong>Results: </strong>NanoSe increased (p<0.05) milk yield compared to the control and SS. In milk, both SS and nanoSe increased (p<0.05) the concentrations of selenium, selenocysteine, and selenomethionine, and the activities of glutathione peroxidase 4, glutathione reductase, thioredoxin reductase, glutathione S-transferase, and iodothyronine deiodinases (type 2 and 3). The nanoSe showed higher (p<0.05) effects on these parameters than SS. Also, dietary nanoSe upregulated (p<0.05) the mRNA expressions of selenoproteins P, W, S, F, M, N, K, O, H, and I in milk compared to the control. For most selenoproteins, there was no difference between SS and nanoSe, only selenoprotein K was higher (p<0.05) in nanoSe than in SS.</p><p><strong>Conclusion: </strong>In conclusion, dietary nanoSe increased milk yield, milk selenoamino acids and selenoproteins in peripartal dairy cows.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1175-1181"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal dynamics of plasma bile acids and their associations with physiological parameters and fecal microbiome during the transition period in dairy cows.","authors":"Feixiang Fan, Liang Chen, Huizeng Sun, Jianxin Liu, Kailun Yang, Fengfei Gu","doi":"10.5713/ab.24.0628","DOIUrl":"10.5713/ab.24.0628","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study is to investigate the dynamic changes of plasma bile acids (BA) and their correlations with physiological parameters and fecal microbiome in transitional dairy cows.</p><p><strong>Methods: </strong>Twenty multiparous dairy cows were selected, the blood and fecal samples were collected on d -21, -7, +7, and +21 of calving. The targeted metabolome and 16s rDNA ampicon sequencing were utilized to identify BA profiles and fecal microbial composition, respectively.</p><p><strong>Results: </strong>A total of 32 BAs were found, comprising 9 primary BAs (PBA) and 23 secondary BAs (SBA). Majority of the PBAs (7 out to 9) and SBAs (15 out to 23) exhibited significant increases postpartum compared to prepartum levels. Notably, ursodeoxycholic acid, taurocholic acid and 7-ketodeoxycholic acid showed higher importance. Correlation analysis showed the BAs concentrations positively correlated with the concentrations of aspartate aminotransferase, total antioxidant capacity, and glutathione peroxidase, while exhibiting substantial negative correlation with triglyceride concentrations. A decline in bacterial alpha diversity in postpartum and significantly different β-diversity were observed. Furthermore, 30 distingtive genera were identified over the transition period. Among these, six and eleven biomarkers such as Alistipes and Ruminococcaceae_UCG_014 were identified at +7 d and +21 d, respectively. Furthermore, the abundances of choloylglycine hydrolase and 7-alpha-hydroxysteroid dehydrogenase which are involved in SBA biosynthesis were significantly higher postpartum as determined by PICRUSt2 analysis over the transition period.</p><p><strong>Conclusion: </strong>The BA profile and concentrations underwent significant changes during the transition period in dairy cows and these changes are closely related to the periparturient health of the cows. Ursodeoxycholic acid and Alistipes was identified as the pivotal BA and microbial genus. Our study elucidates these metabolic processes, providing useful insights into strategies for enhancing the nutrition and well-being of perinatal dairy cows.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1194-1205"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of calcium lactate on in vitro fertilization and embryonic development in cattle.","authors":"Bo-Myeong Kim, Song-Hee Lee, Geun Heo, Ji-Dam Kim, Gyu-Hyun Lee, Jae-Min Sim, Kwang Taek Lim, Xiang-Shun Cui","doi":"10.5713/ab.24.0636","DOIUrl":"10.5713/ab.24.0636","url":null,"abstract":"<p><strong>Objective: </strong>Growing demand for embryo transfer is steadily expanding and further studies on in vitro fertilization of cattle. To assess the effect of calcium lactate by replacing Tyrode's albumin lactate pyruvate (TALP) medium composition during fertilization and embryonic development.</p><p><strong>Methods: </strong>Sodium lactate and CaCl2 were replaced with 2.0, 3.0, 4.5 mM calcium lactate for TALP medium during fertilization in Experiment 1. In Experiment 2, the concentrations of sodium lactate and CaCl2 were re-modified as control, in comparison with the same concentration of calcium lactate at 4.5 mM. Zygotes were moved to sequential media to match early-and late-stage environments. Embryonic development was examined on day 8 after insemination.</p><p><strong>Results: </strong>A 4.5 mM calcium lactate enhanced the rate of fertilization and blastocyst formation (p<0.0001, p<0.01, respectively). It represented differences in the reactive oxygen species (ROS) (p<0.01) and glutathione (GSH) levels (p<0.05) and increased blastocyst diameter and total cell number (p<0.05). In Experiment 2, fertilization (p<0.05) and blastocyst formation rates (p<0.01) were increased in 4.5mM calcium lactate under same concentration effect of sodium lactate and CaCl2. Additionally, it reduced the ROS (p<0.01) and increased the GSH levels (p<0.05), leading increase embryo quality.</p><p><strong>Conclusion: </strong>The replacement of calcium lactate in TALP medium enhances fertilization and embryonic development while also improving oxidative stress. Specifically, it has been determined that a concentration of 4.5 mM calcium lactate is the most effective, irrespective of the varying concentrations of sodium lactate and CaCl2. This study presents a novel formulation of a modified TALP medium intended for implantation withing the bovine embryo industry. The current implications of the study are discussed in relation to previously stated objectives and hypotheses.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1150-1159"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061575/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}