Animal Bioscience最新文献

{"title":"Comparative study on the effects of glutamic acid and glutamine in promoting intestinal development in chicks through energy metabolism.","authors":"Peiyu Huang, Yaoming Cui, Wenjing Liang, Li Zhang, Junquan Tian, Liping Gan, Linna Guo, Weiyu Chen, Guohao Yang, Junjun Guan","doi":"10.5713/ab.25.0445","DOIUrl":"https://doi.org/10.5713/ab.25.0445","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the effects of glutamic acid (Glu) and glutamine (Gln) on the intestinal development of layer chicks with lipopolysaccharide (LPS)-induced damage.</p><p><strong>Methods: </strong>A total of 240 healthy 0-d-old Hy-Line Brown chicks were randomly assigned to 4 treatments, each with 6 replicates. At 8 and 11 d of age, all birds (except for the control group) received two administrations of LPS. The LPS-challenged birds were divided into three dietary treatment groups: a basal diet (without additives), a 0.05% Glu-supplemented diet, and a 0.20% Gln-supplemented diet.</p><p><strong>Results: </strong>The LPS challenge induced intestinal injury and suppressed intestinal development in layer chicks, as evidenced by significantly reduced growth performance, poor intestinal parameters, and morphology (p<0.05). Compared to the LPS group, dietary supplementation with 0.05% Glu and 0.20% Gln significantly enhanced average daily gain (ADG), average daily feed intake (ADFI), body weight (BW), and intestinal development parameters (including length, weight, villus height, villus height/crypt depth) of duodenum, jejunum and ileum (p<0.05). These results could be attributed to upregulated mRNA expression levels of Mucin-2, E-cadherin, Dclk-1, Vil-1, Lysozyme, ChgA, Lgr-5, Bmi-1, ATP5F1AZ, and β-catenin (p<0.05). Furthermore, dietary supplementation with 0.20% Gln outperformed 0.05% Glu in enhancing BW, ADG, and ileum parameters (weight, length, epithelial cell count, and energy metabolism) (p<0.05). Additionally, intestinal organoids supplemented with 10 μM Gln had significantly higher mean area, E-cadherin gene expression, and ATP content compared with those treated with 5 μM Glu in vitro (p<0.05).</p><p><strong>Conclusion: </strong>Dietary supplementation with 0.05% Glu and 0.20% Gln could improve growth performance, intestinal development, and repair intestinal damage in layer chicks through enhanced epithelial proliferation and differentiation. Moreover, 0.20% Gln performed better than 0.05% Glu, which may be attributed to superior energy metabolism.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction models established for net energy and standardized ileal digestible amino acids in regionally sourced fermented soybean meal for growing pigs.","authors":"Wenjun Gao, Qile Hu, Yingying Li, Hongrui Cao, Xue Bao, Renjie Wang, Shuai Zhang","doi":"10.5713/ab.25.0485","DOIUrl":"https://doi.org/10.5713/ab.25.0485","url":null,"abstract":"<p><strong>Objective: </strong>The study was conducted to determine the available energy and standardized ileal digestibility (SID) of amino acid (AA) in fermented soybean meal (FSBM), and establish prediction equations for growing pigs.</p><p><strong>Methods: </strong>In Exp. 1 to determine available energy, twenty-four growing barrows (initial body weight = 35.3 ± 3.2 kg) were randomly assigned to two replicated 6 × 6 Latin square designs, each comprising one corn-based basal diet (used in both squares) and five test diets. In Exp. 2 on AA digestibility, twenty-two growing barrows (initial body weight: 48.8 ± 2.8 kg) underwent distal ileal T-cannulation and were arranged to a 3-period crossover design with one nitrogen-free diet and 10 test diets. The inclusion levels of FSBM in the test diets of Exp. 1 and Exp. 2 were 27.49% and 40%, respectively.</p><p><strong>Results: </strong>The coefficients of variation among FSBM for ether extract (EE), crude fiber (CF), neutral detergent fiber (NDF), and acid detergent fiber (ADF) all exceeded 10%. The digestible energy, metabolizable energy, and net energy (NE) values of FSBM ranged from 15.50 to 18.44 MJ/kg dry matter (DM), 13.98 to 16.72 MJ/kg DM, and 10.10 to 11.05 MJ/kg DM, respectively. The SID values of AA demonstrated variation (p<0.05) for most AA, with the exception of Arg and Lys among indispensable AA, and Glu, Ser, and Tyr among dispensable AA. The best-fitted prediction equation for NE was a model incorporating EE, NDF, and GE (R² = 0.92, p<0.01), while the best-fitted equations for SIDCrude Protein and SIDLys incorporated DM, NDF, and ADF (R² = 0.71, p = 0.097) and CF, ADF, and Ash (R² = 0.83, p = 0.022), respectively.</p><p><strong>Conclusion: </strong>The NE values of FSBM ranged from 10.10 to 11.05 MJ/kg DM. The NE value, SIDCrude Protein, and SIDLys of FSBM can be well predicted based on nutritional parameters.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplementation of black soldier fly larvae (Hermetia illucens) as a sustainable protein source on growth performance, blood profiles, immune response, and diarrhea incidence in weaning pigs.","authors":"Noh Soo Duc, Jin Xing Hao, Jang Min Hyuk, Park Min Soo, Kim Yoo Yong","doi":"10.5713/ab.25.0425","DOIUrl":"https://doi.org/10.5713/ab.25.0425","url":null,"abstract":"<p><strong>Purpose: </strong>This study investigates the potential of replacing fishmeal with black soldier fly larvae (BSF) in weaning pig diets, aiming to overcome the limitations of traditional animal protein sources and explore BSF larvae as a sustainable alternative protein.</p><p><strong>Methods: </strong>A total of 192 weaning ([Yorkshire × Landrace]) × Duroc) pigs (8.12 ± 0.01 kg body weight [BW]) were assigned to four treatments based on sex and initial body weight, with six replicates of eight pigs per pen in a randomized complete block design (RCBD). Experimental diets with different levels of black soldier fly (BSF) larvae were as follows: 1) Control: corn‒soybean-based diet, 2) BSF25: corn‒soybean-based diet containing black solder fly larvae as a replacement for 25% of plasma protein, 3) BSF50: corn‒soybean-based diet containing black solder fly larvae as a replacement for 50% of plasma protein, 4) BSF100: corn‒soybean-based diet containing black solder fly larvae as a replacement for 100% of plasma protein.</p><p><strong>Results: </strong>During phase I, the average daily gain (ADG) and gain: feed (G:F) ratio were decreased as the BSF larvae level increased (linear, p=0.03; p=0.03), and the BSF25 group had higher body weight compared to other groups (anova, p=0.01). In blood profiles, increasing levels of BSF larvae in the diet linearly decreased albumin levels at 28 d after weaning (linear, p=0.01). For immune response, there was a linear decrease in IgG concentration by increasing levels of BSF larvae (linear, p=0.05), and the BSF25 group had the highest value among all treatments only in phase I (anova, p=0.05).</p><p><strong>Conclusion: </strong>The results of this study demonstrate that BSF larvae meal can be a viable alternative protein source in diets for weaned pigs. The optimal ratio of BSF larvae replacing plasma protein is up to 25%, which leads to improved growth performance and immune response.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary 25-hydroxycholecalciferol supplementation on growth performance, blood vitamin D and antioxidant status in nursery pigs.","authors":"Chan Ho Kwon, Eva S Safaie, Savannah L Locke, Jannell A Torres, Zhaohui Yang, Xi Chen, Young Dal Jang","doi":"10.5713/ab.25.0029","DOIUrl":"10.5713/ab.25.0029","url":null,"abstract":"<p><strong>Objective: </strong>This study was conducted to evaluate the effect of dietary 25-hydroxycholecalciferol (25-OHD3) supplementation on growth performance, blood 25-OHD3 level, and antioxidant parameters in nursery pigs.</p><p><strong>Methods: </strong>A total of 48 newly weaned piglets (5.27±1.42 kg initial body weight) were allotted to 3 treatments in 4 replicates with 4 pigs per pen for a 28-d feeding trial in two phases for d 0-14 (Phase 1) and d 14-28 (Phase 2) postweaning with basal diets without supplemental vitamin D3 (VD3). Treatments were: 1) basal diet+2,000 IU/kg VD3 supplementation, 2) basal diet+1,000 IU/kg 25-OHD3 supplementation, and 3) basal diet+2,000 IU/kg 25-OHD3 supplementation. Growth performance, plasma 25-OHD3 and malondialdehyde (MDA) levels, total antioxidant capacity, and superoxide dismutase activity were measured.</p><p><strong>Results: </strong>There was no significant difference in growth performance among dietary treatments until d 21 postweaning. Dietary 25-OHD3 supplementation increased feed intake (p<0.07) and growth rate (p<0.05) greater than the VD3 treatment in d 21-28 and d 14-28 postweaning, resulting in a greater growth rate in the overall period (p = 0.10). Increasing levels of 25-OHD3 supplementation resulted in greater plasma 25-OHD3 concentrations at d 14 and 28 postweaning (p<0.05), while decreasing plasma MDA levels at d 28 postweaning (p<0.05) with no differences in plasma superoxide dismutase activity and total antioxidant capacity. In the broken-line analysis, the estimated plasma 25-OHD3 concentration for plasma MDA concentration to reach the minimum level was 23.7 ng/mL (p<0.05).</p><p><strong>Conclusion: </strong>Supplementing 25-OHD3 in nursery diets increased blood vitamin D status and had potential to enhance feed intake and growth rate in the late nursery period, while reducing oxidative stress compared with VD3 supplementation.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1943-1952"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of sodium alginate active films containing different lotus rhizome root powders on physicochemical properties and shelf-life of pork patties.","authors":"Zhuangzhuang Qiu, Koo Bok Chin","doi":"10.5713/ab.25.0028","DOIUrl":"10.5713/ab.25.0028","url":null,"abstract":"<p><strong>Objective: </strong>This study was done to investigate the film properties and antioxidant activities of sodium alginate films (SAFs) added with different levels (0.5, 1, and 2%) of oven-dried (100°C) lotus rhizome root powder (ODLRRP).</p><p><strong>Methods: </strong>After pork patties were wrapped with different SAFs, the physiochemical properties and antimicrobial and antioxidant activities of pork patties were determined.</p><p><strong>Results: </strong>SAFs containing ODLRRP decreased the pH, lightness (L*), and equilibrium water content but increased the redness (a*), yellowness (b*), transparency, moisture content, and antioxidant activities. SAFs containing ODLRRP increased the color values, but L*, 2-thiobarbituric acid-reactive substances, microbial counts, and water content decreased. In pork patties wrapped with SAFs containing ODLRRP at levels greater than 1% ODLRRP, the L*, volatile basic nitrogen, and total bacterial counts decreased, whereas b* increased.</p><p><strong>Conclusion: </strong>These results indicated that pork patties wrapped with SAFs containing more than 1% ODLRRP could inhibit microbial growth and reduce protein denaturation.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2021-2032"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA ST6GALNAC3 inhibits dermal fibroblast proliferation and migration in cashmere goat hair follicles via the chi-miR-24-3p/ID4 axis.","authors":"Rong Ma, Qing Ma, Lu Zhang, Bingjie Ma, Xuxu Bao, Yiming Zhang, Le Wang, Qi Lv, Zhiying Wang, Ruijun Wang, Rui Su, Yanhong Zhao, Fangzheng Shang, Yu Wang, Yanjun Zhang","doi":"10.5713/ab.25.0115","DOIUrl":"10.5713/ab.25.0115","url":null,"abstract":"<p><strong>Objective: </strong>Dermal papilla is developed from the continuous proliferation and differentiation of dermal fibroblasts, which is the key to the normal development of hair follicles. This study aims to elucidate the role of lncRNA ST6GALNAC3, which is significantly differentially expressed during the secondary hair follicle development stage in cashmere goats, on dermal fibroblasts, and to analyze the regulatory mechanism of this lncRNA thoroughly.</p><p><strong>Methods: </strong>We conducted a screening process and characterization for lncRNAs associated with the development of secondary hair follicles. The effects of lncRNA ST6GALNAC3 on cell proliferation and migration were assessed using CCK8, EdU, and flow cytometry. Subsequently, we employed bioinformatics analysis to identify the target miRNAs of lncRNA ST6GALNAC3 and the corresponding target genes of these miRNAs, respectively, and initially constructed the regulatory axis of lncRNA ST6GALNAC3-chi-miR-24-3p-ID4. Luciferase reporter assays and rescue experiments were performed to confirm the regulatory axis at both molecular and cellular levels, thus elucidating the mechanism by which lncRNA ST6GALNAC3 regulates dermal fibroblasts.</p><p><strong>Results: </strong>One hundred fifty-eight lncRNAs related to secondary hair follicle morphogenesis were identified. Among them, lncRNA ST6GALNAC3 was significantly differentially expressed on embryonic day 75 and significantly inhibited the proliferation and migration of dermal fibroblasts. The results showed that lncRNA ST6GALNAC3 can target chi-miR-24-3p, which in turn can target the ID4 gene. The results of the luciferase reporter assay and rescue assay showed that chi-miR-24-3p binds to both lncRNA ST6GALNAC3 and ID4. Furthermore, lncRNA ST6GALNAC3 can indirectly regulate the proliferation and migration of dermal fibroblasts through chi-miR-24-3p/ID4 axis.</p><p><strong>Conclusion: </strong>LncRNA ST6GALNAC3 inhibits the proliferation and migration of dermal fibroblasts through the chi-miR-24-3p/ID4 axis, thus suppressing the formation of dermal papilla structures and influencing the morphogenesis of secondary hair follicles during embryonic development.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1869-1880"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parental betaine supplementation promotes hepatic conversion of cholesterol to bile acids in offspring goslings with epigenetic modulation of CYP7A1 gene.","authors":"Shuai Ma, Liang Chen, Yan Wang, Lei Wu, Ruqian Zhao","doi":"10.5713/ab.24.0857","DOIUrl":"10.5713/ab.24.0857","url":null,"abstract":"<p><strong>Objective: </strong>Maternal betaine supplementation has been shown to affect offspring metabolism through epigenetic mechanisms in mammals. This study aimed to investigate whether parental betaine supplementation can exert intergenerational effects on hepatic cholesterol metabolism in offspring goslings, and the possible epigenetic modification associated with such effects.</p><p><strong>Methods: </strong>In this study, 450 female and 90 male Jiangnan White goose breeders, aged 39 weeks, were randomly assigned to three groups receiving basal (control, CON), or betaine-supplemented diets at low (LBT, 2.5 g/kg) or high (HBT, 5 g/kg) levels for 7 weeks. The breeder eggs laid in the last week were collected for incubation. Offspring goslings were slaughtered at 35 and 63 days of age to collect blood, bile, and liver samples for biochemical analysis and gene and protein expression studies.</p><p><strong>Results: </strong>The body weight of goslings in the LBT and HBT groups was significantly higher than that in the CON group (p<0.05). Serum and liver total cholesterol contents were significantly decreased in the HBT group (p<0.05), while the total bile acids contents in the liver and bile were significantly increased (p<0.05). These changes were associated with the upregulation of cholesterol-7 alpha-hydroxylase (CYP7A1) and bile salt export protein at both mRNA and protein levels (p<0.05). Additionally, parental betaine supplementation significantly decreased the DNA methylation level on the promoter region of the CYP7A1 gene (p<0.05).</p><p><strong>Conclusion: </strong>These results indicate that parental betaine supplementation decreases hepatic cholesterol content in offspring goslings through epigenetic modulation of the CYP7A1 gene.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1973-1983"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of probiotic supplementation and rabbit line on growth performance, carcass yield, blood biochemistry and immune response under hot weather.","authors":"Magdy Abdelsalam, Moataz Fathi, Alaa El-Raffa, Ghada Abd El-Latif, Osama Abou-Emera, Mohamed Abd El-Fatah, Gamal Rayan","doi":"10.5713/ab.24.0904","DOIUrl":"10.5713/ab.24.0904","url":null,"abstract":"<p><strong>Objective: </strong>An experiment was conducted to investigate the beneficial effect of probiotic supplementation via drinking water on growth performance, carcass yield, blood parameters, and immunity of two rabbit lines during the summer season.</p><p><strong>Methods: </strong>In a 6-week probiotic supplementation trial, a total of 90 healthy growing rabbits having a similar weight (590.3±16 g) representing two lines (V-Line and Alexandria) were randomly allocated into three equal groups. Three levels of probiotics (0, 6.67×109 colony forming unit [CFU]/L and 13.35×109 CFU/L of Bacillus licheniformis) were administered through drinking water as non-supplemented, low, and high levels.</p><p><strong>Results: </strong>A linearly increased body weight at 5 and 6 weeks of the experiment (p = 0.01 and p<0.01, respectively) were noticed with the probiotic supplementation at high level. The same trend was noticed for the overall body weight gain (p = 0.05). Probiotic supplementation did not affect carcass traits, except for heart and lungs, where the rabbits that received the high level recorded higher weights (p = 0.01 and p<0.01, respectively). Probiotic supplementation showed a linearly effect (p = 0.02) on the cell-mediated index after 24 h of phytohemagglutinin injection. Lower triglyceride concentration (p = 0.04) was observed in the high group compared to the other groups. Concerning the line rabbit, although the Alexandria rabbits (Alex) recorded lighter body weight, they exhibited a better gain-to-feed ratio (p = 0.05) compared to the V-line ones. Also, an increase in heart weight and spleen index (p<0.01) was found in V-Line rabbits compared to the Alex ones. Contrary, the Alex rabbits recorded a higher kidney weight (p<0.01) than that of V-Line. Overall, no difference was recorded between the rabbit lines in immune response.</p><p><strong>Conclusion: </strong>The beneficial effects of probiotic supplementation via drinking water on rabbit performance under hot weather were identified as improved growth performance, an enhanced lipid profile, and an early increase in cell-mediated immunity in both rabbit lines that received a high level of the probiotics.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2033-2042"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiRNA-24 downregulates KLF6 affecting STAT3 protein expression and phosphorylation regulating melanogenesis in cashmere goat coat.","authors":"Baoyu Zhang, Runlai Liu, Yuxin Zhao, Xinyu Li, Huixian Su, Shufang Li, Jianping Li, Huaizhi Jiang, Qiaoling Zhang","doi":"10.5713/ab.24.0824","DOIUrl":"10.5713/ab.24.0824","url":null,"abstract":"<p><strong>Objective: </strong>This study revealed that miRNA-24 downregulates KLF6, regulating melanogenesis in the coat of Cashmere goats.</p><p><strong>Methods: </strong>The correlation between miRNA-24 and coat color was determined by quantitative reverse transcription polymerase chain reaction (RT-qPCR). GO/KEGG analysis and bioinformatics tools were used to screen for target genes. Targeted interactions were confirmed using the dual luciferase assay. The expressions of miRNA-24 and KLF6 on different colored skins of Cashmere goats, and the KLF6 expression in B16-F10 cells transfected with miRNA-24 mimics/inhibitors/NC were detected by RT-qPCR, Western Blot, and Immunofluorescence. To investigate the pathway that KLF6 influenced, KLF6 was silenced by RNA interference (RNAi), and the melanogenesis pathway's gene expression was examined. The expression and phosphorylation of STAT3 protein in the pathway were inhibited. RNAi and Stattic were utilized to downregulate its protein expression and phosphorylation for detecting tyrosine expression. BALB/c mice were subcutaneously injected with antagomiRNA-24, a miRNA-24 inhibitor. The influence of miRNA-24 on the downregulation of KLF6 in relation to melanogenesis was examined.</p><p><strong>Results: </strong>MiRNA-24 is related to coat color. KLF6 was chosen as the target gene, and the targeted interaction was validated. The expression trends of miRNA-24 and KLF6 in the skin inversely correlated. The mRNA and protein expression of KLF6 were altered in B16-F10 cells after transfected. These suggested that miRNA-24 downregulates KLF6. Silencing of KLF6 resulted in the downregulation of genes linked to melanogenesis, including WNT1, WNT2, PRKACA, MAPK1, TYR, and STAT3. The suppression of protein and phosphorylation levels of STAT3 decreaseed the mRNA level of TYR, suggesting that KLF6 influences TYR expression by modulation of STAT3 expression and phosphorylation. BALB/c mice injected with antagomiRNA-24 showed increased melanin content and decreased KLF6 expression.</p><p><strong>Conclusion: </strong>MiRNA-24 regulates melanogenesis in the Cashmere goat coat by downregulation of KLF6, thus mediating STAT3 expression and phosphorylation.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1984-1995"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of imputation performance based on the single nucleotide polymorphism panel density and the reference population size in Korean native chicken.","authors":"Minjun Kim, Hyo Jun Choo, Sunghyun Cho, Doo Ho Lee, Jun Heon Lee, Dongwon Seo","doi":"10.5713/ab.24.0815","DOIUrl":"10.5713/ab.24.0815","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to identify the optimal single nucleotide polymorphism (SNP) panel density for accurate imputation in the Korean native chicken (KNC) and Yeonsan Ogye (YO) populations. The primary focus was on evaluating how the reference population size and SNP density influence imputation performance and accuracy.</p><p><strong>Methods: </strong>Data were collected from five purebred lines of KNC and the YO population, comprising a total of 256 KNC and 199 YO chickens. Imputed genotype ratio and accuracy were evaluated across various scenarios using SNP densities of 2.5K, 5K, 10K, and 50K in both populations. Additionally, for the YO dataset, reference population sizes of 50, 100, and 150 were analyzed to assess their impact on imputation outcomes.</p><p><strong>Results: </strong>Higher SNP densities notably improved imputation performance. Specifically, when SNP panel density reached 10K or greater, the ratio of imputed SNPs exceeded 70% and the accuracy increased substantially, regardless of the reference population size. However, imputation efficiency decreased markedly when either the reference or test population size was around 50 individuals.</p><p><strong>Conclusion: </strong>A test SNP density of at least 10K was determined to be essential for accurate genotype imputation. Additionally, imputation efficiency was observed to decline when either the reference or test population included around 50 individuals. These findings provide important data that can guide the genetic improvement of indigenous livestock populations.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"1858-1868"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}