Animal Bioscience最新文献

{"title":"- Invited Review - Challenges and constraints to the sustainability of poultry farming in Thailand.","authors":"Sawitree Wongtangtintharn, Sudpradthana Chakkhambang, Padsakorn Pootthachaya, Anusorn Cherdthong, Metha Wanapat","doi":"10.5713/ab.24.0685","DOIUrl":"10.5713/ab.24.0685","url":null,"abstract":"<p><p>The poultry farming industry in Thailand plays a crucial role in the nation's economy and food security. However, its long-term sustainability is challenged by disease outbreaks, environmental concerns, rising feed costs, market fluctuations, and shifting consumer preferences. This review examines the current state of poultry farming in Thailand, focusing on broilers, layers, and ducks. Disease control requires strict biosecurity measures and government interventions. Additionally, the industry must address environmental concerns by reducing greenhouse gas emissions and optimizing resource efficiency to align with Thailand's net-zero targets. The rising demand for organic and ethically produced poultry products presents both market opportunities and production challenges, necessitating significant adjustments in farming practices. Regulatory compliance, particularly in export markets, adds another layer of complexity, requiring Thai poultry producers to meet increasingly stringent international standards. The adoption of new technologies, such as precision farming and artificial intelligence-driven systems, presents both opportunities and challenges, especially for smaller producers who may struggle to keep pace with advancements. Rising feed costs, driven by global supply chain disruptions and dependence on imported raw materials, pose a significant economic burden, emphasizing the need for alternative protein sources such as insect-based feed and agro-industrial by-products. Furthermore, regulatory compliance with international standards and consumer-driven trends toward antibiotic-free and organic poultry products further shape the industry's transformation. To ensure sustainable development, Thailand's poultry sector must integrate innovative technologies, adopt environmentally friendly practices, and strengthen collaboration between the government, industry stakeholders, and researchers. The industry can maintain its global competitiveness by addressing these challenges while contributing to sustainable food production and environmental conservation.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"845-862"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969231/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guanidinoacetic acid regulated postmortem muscle glycolysis associated with AMPK signaling and protein acetylation.","authors":"Ning Liu, Bolin Zhang, Shubai Wang, Qingzhen Zhong, Zewei Sun","doi":"10.5713/ab.24.0418","DOIUrl":"10.5713/ab.24.0418","url":null,"abstract":"<p><strong>Objective: </strong>Antemortem stress accelerated muscle energy consumption in postmortem muscle. The objective of our study was to investigate the regulation of guanidinoacetic acid (GAA) administration on the postmortem glycolysis and protein acetylation in postmortem muscle of antemortem stress.</p><p><strong>Methods: </strong>Forty C57BL/6 male mice were chosen and randomly assigned to four treatment groups (A, B, C and D), each treatment consisted of 10 replicates. Mice in group B, C and D were treated with 0.05% GAA oral administration for 6 days. On the 7th day of the experiment, the mice in group A and B were injected with saline, and mice in group C and D were injected with 5-aminoimidazole-4-carboxamide1-β-D-ribofuranoside (AICAR; 50 μg/g body weight) and a combined injection with AICAR (50 μg/g body weight) and histone acetylase inhibitor II (HAT II; 185 μg/g body weight), respectively.</p><p><strong>Results: </strong>The results showed that the values of pH45min and pH24h of postmortem muscle in GAA administration were higher than those in the control group. However, the opposite result was observed in AICAR group. Moreover, the activities of pyruvate kinase, hexokinase and fruc-tose-2,6-diphosphatase, combined with the protein abundance of phosphorylated liver kinase, phosphorylated AMPKα2 and total acetylated protein were all decreased by GAA administration and HAT II treatment.</p><p><strong>Conclusion: </strong>Taken together, adenosine 5'-monophosphate-activated protein kinase signaling and protein acetylation could mediate the regulation of GAA administration on postmortem glycolysis of antemortem stress-muscle.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"765-774"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"- Invited Review - Challenges and constraints to the sustainability of poultry farming in Indonesia.","authors":"Sumiati, Roni Fadilah, Arif Darmawan, Rizky Nadia","doi":"10.5713/ab.24.0678","DOIUrl":"10.5713/ab.24.0678","url":null,"abstract":"<p><p>Although the demand for poultry products in Indonesia, one of the most significant poultry-producing countries in Southeast Asia, continues to increase, the poultry farming sector continues to face various challenges. This paper provides an overview of the prospects for and challenges faced by the poultry farming sector in Indonesia. Broilers comprise the largest portion of Indonesia's poultry population, with other commodities contributing less than 10% of the total. During the COVID-19 pandemic, the consumption of chicken meat and eggs increased. The declining national economy led people to consume affordable food for their daily meals, favoring chicken and eggs because their retail prices were four times cheaper than that of beef. In terms of imports to Indonesia, compared with other agricultural commodities, poultry and eggs account for only approximately 1.26% and 0.41%, respectively. Meanwhile, some feed ingredients, such as soybean meal and corn, continue to be imported into Indonesia. According to data from the United States Department of Agriculture, Indonesia imported 4.4 million tons of soybean meal to meet the needs of its poultry industry. In recent years, feed mill companies have rapidly been developing in Indonesia, with an estimated average production of 572,000 metric tons. In terms of scale, poultry farming in Indonesia comprises 60% large-scale farming and 40% small- and medium-scale farming. Many small-scale farms have yet to implement strict biosecurity measures, making climate change a significant challenge for poultry farming in Indonesia. After implementing the ban on antibiotic growth promoters, Indonesia's poultry farming sector began to stabilize and look for alternative methods to maintain productivity. The use of technology, improved regulations, and the enhancement of farmers' capacity to manage challenges are key toward sustaining and increasing poultry farming productivity in Indonesia in the future.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"802-817"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oleanolic acid improves the in vitro developmental competence of early porcine embryos by reducing oxidative stress and ameliorating mitochondrial function.","authors":"Yan-Wei Dong, He-Xuan Qu, Yan-Qiu Wang, Jia-Jia Qi, Hua-Kai Wei, Bo-Xing Sun, Hao Sun, Shuang Liang","doi":"10.5713/ab.24.0307","DOIUrl":"10.5713/ab.24.0307","url":null,"abstract":"<p><strong>Objective: </strong>Oleanolic acid (OA) is a pentacyclic triterpenoid with antioxidant activity that can be an effective scavenger of free radicals in cells. This study was designed to investigate the effects of OA on porcine early embryo developmental competence in vitro and its possible mechanisms of action.</p><p><strong>Methods: </strong>In the present study, parthenogenetically activated porcine embryos were used as models to assess the effects of OA on the in vitro developmental capacity of early porcine embryos in vitro. Zygotic genome activation, mitochondrial function, oxidative stress, cell proliferation and apoptosis in early porcine embryos were examined after supplementing the culture medium with 5 μM OA.</p><p><strong>Results: </strong>The results showed that 5 μM OA supplementation not only significantly increased the blastocyst diameter in early embryos on day 6 but also increased the total cell number of blastocysts. Furthermore, OA supplementation increased the blastocyst proliferation rate and decreased blastocyst apoptosis. Moreover, OA supplementation significantly increased the proportion of embryos that developed to the 4-cell stage after 48 h of in vitro culture and upregulated the expression of genes associated with zygotic genome activation (DPPA2 and ZSCAN4). Notably, OA alleviated oxidative stress by reducing the intracellular levels of reactive oxygen species and increasing the intracellular levels of reduced glutathione at the 4-cell stage and increased the activities of superoxide dismutase and catalase. Concurrently, OA significantly increased the mitochondrial membrane potential and intracellular adenosine 5'-triphosphate content.</p><p><strong>Conclusion: </strong>These results suggest that OA promotes the in vitro developmental competence of parthenogenetically activated porcine embryos by reducing oxidative stress and improving mitochondrial function during in vitro culture and that OA may contribute to the efficiency of in vitro embryo production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"431-443"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advantageous effects of rumen-protected phytonutrients from tropical plant extracts on rumen fermentation efficiency and methane mitigation using in vitro fermentation technique.","authors":"Maharach Matra, Chaichana Suriyapha, Gamonmas Dagaew, Rittikeard Prachumchai, Srisan Phupaboon, Sukruthai Sommai, Metha Wanapat","doi":"10.5713/ab.24.0576","DOIUrl":"10.5713/ab.24.0576","url":null,"abstract":"<p><strong>Objective: </strong>Tropical plants are composed of phytonutrients (PTNs) and are utilized for their capacity to manipulate rumen fermentation characteristics and methane production. The aim of this experiment was to determine the impact of microencapsulated PTNs-extracted from lemongrass and mangosteen peel (M-LEMANGOS), as well as crude protein (CP) levels on nutrient degradability, rumen ecology, microbial population, and methane emission in an in vitro study.</p><p><strong>Methods: </strong>The treatments were randomly assigned in a 2×4 Factorial arrangement in a completely randomized design. The two factors consisted of CP percentage in the concentrate diet (16% and 18% CP) and the levels of M-LEMANGOS addition (0%, 2%, 4%, and 6% of the total substrate).</p><p><strong>Results: </strong>The results showed that nutrient degradability both 12 and 24 h were significantly increased with M-LEMANGOS at 4% total substate. In part of volatile fatty acids (VFAs), particularly propionate and total VFA, these were enhanced by %CP and M-LEMANGOS combination. The %CP increased ruminal ammonia-nitrogen concentration (NH3-N), while M-LEMANGOS supplementation reduced such concentration. Methane production and Methanobacteriales population at 12 and 24 h were reduced when supplemented with M-LEMANGOS at 4% total substate. The population of Fibrobacter succinogenes, Ruminococcus flavefaciens, and Megasphaera elsdenii were increased with the interaction between %CP and M-LEMANGOS addition.</p><p><strong>Conclusion: </strong>M-LEMANGOS indicates promising potential as a plant-based PTN for dietary modulation of rumen fermentation and mitigation of methane production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"489-500"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The decrease in Rad51 and DNA ligase IV nuclear protein expression in Msh2 knockdown HC11 cells induced the low CRISPR/Cas9-mediated knock-in efficiency at the β-casein gene locus.","authors":"Ga-Yeon Kim, Man-Jong Kang","doi":"10.5713/ab.24.0206","DOIUrl":"10.5713/ab.24.0206","url":null,"abstract":"<p><strong>Objective: </strong>Successful gene editing technology is crucial in molecular biology and related fields. An essential part of an efficient knock-in system is increasing homologous recombination (HR) efficiency in the double-strand break (DSB) repair pathways. Interestingly, HR is closely related to the DNA mismatch repair (MMR) pathway, whereby MMR-related gene Msh2 recognizes a mismatch of nucleotides in recombinant intermediates or gene conversion formed during HR. This study aimed to investigate how the knockdown of Msh2 affects HR-mediated knock-in efficiency at the mouse β-casein locus. Therefore, we investigated the effect of inhibiting Msh2 expression on the expression of the HR-related gene Rad51 and the key enzyme DNA ligase IV involved in non-homologous end joining (NHEJ).</p><p><strong>Methods: </strong>The knock-in vector targeting the mouse β-casein gene locus, programmed guide RNA, and Msh2 siRNA expression vector were co-transfected in HC11 cells, or only the Msh2 siRNA expression vector was transfected. Knock-in efficiency was confirmed by polymerase chain reaction (PCR). The mRNA and protein expression of Msh2, HR-related gene Rad51, and NHEJ-related gene DNA ligase IV were evaluated by quantitative reverse transcription PCR (RT-qPCR) and Western blot analysis.</p><p><strong>Results: </strong>The knock-in vector efficiency at the mouse β-casein gene locus significantly decreased upon Msh2 knockdown in HC11 mouse mammary epithelial cells (HC11 cell). Additionally, the knockdown of the DNA MMR-related gene Msh2 protein significantly downregulated the nuclear protein expression of the HR-related Rad51 and NHEJ-related DNA ligase IV genes.</p><p><strong>Conclusion: </strong>The decreased Msh2 protein expression in the nucleus downregulated the Rad51 and ligase IV protein expressions. Consequently, reduced Rad51 expression results in decreased knock-in efficiency.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"560-567"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased apoptosis in late-developing in vitro fertilized bovine blastocysts decreases successful pregnancy.","authors":"Tae-Gyun Kim, Yong-Ho Choe, Sung-Ho Kim, Sang-Yup Lee, Min Jang, Sung-Ho Yun, Seung-Joon Kim, Sung-Lim Lee, Won-Jae Lee","doi":"10.5713/ab.24.0454","DOIUrl":"10.5713/ab.24.0454","url":null,"abstract":"<p><strong>Objective: </strong>Pregnancy in cattle after embryo transfer (ET) is influenced by several factors, including embryo quality. Therefore, preparing high-quality embryos with the greatest developmental potential is essential for achieving a successful pregnancy after ET. Meanwhile, blastocysts produced by in vitro fertilization (IVF) procedure have different developmental speed during in vitro culture (IVC) and they exhibited different competence in the establishment of pregnancy.</p><p><strong>Methods: </strong>This study aimed to identify the comparative features of early-, mid-, and late-developing bovine IVF blastocysts, when they first appeared at Day 7, 8, and 9 during IVC, respectively. In addition, the correlations between their molecular features and pregnancy ability were analyzed.</p><p><strong>Results: </strong>The results showed no difference in the morphological characteristics, including total cell count and diameter, between the Day 7, 8, and 9 blastocysts. However, the pregnancy rate post-ET was significantly different between the groups at 51.7%, 36.7%, and 17.8% for Day 7, 8, and 9 blastocysts, respectively. During early embryo development, late-developing blastocysts demonstrated a reduced cell count in the inner cell mass and decreased expression of the early embryo developmental genes (Oct4 and Sox2) compared with the early- and mid-developing blastocysts. In addition, the number of apoptotic cells and apoptosis-related gene expression (increased Bax and decreased Bcl2) gradually elevated from the Day 7 to Day 9 blastocysts. However, there was no difference in mitochondrial activity and mitochondria-relevant gene expression (Tfam and Cox1) between the groups. Correlation analysis identified a significantly negative correlation between the pregnancy rate and the blastocysts' degree of apoptosis, indicating that the low pregnancy ability of late-developing blastocysts was mainly caused by increased apoptosis.</p><p><strong>Conclusion: </strong>This study's results may contribute to the field of animal biotechnology by assisting in establishing an improved strategy for bovine ET with IVF embryos.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"454-465"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CORRIGENDUM.","authors":"","doi":"10.5713/ab.23.0367C","DOIUrl":"10.5713/ab.23.0367C","url":null,"abstract":"","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":"38 3","pages":"575"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apparent metabolizable energy concentration and ileal amino acid digestibility in cereal grains fed to broiler chickens.","authors":"June Hyeok Yoon, Changsu Kong","doi":"10.5713/ab.24.0398","DOIUrl":"10.5713/ab.24.0398","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to determine apparent metabolizable energy concentrations and ileal amino acid (AA) digestibility in cereal grains and to compare those metabolizable energy values between the total collection and index methods for 21-day-old broilers.</p><p><strong>Methods: </strong>On day 17 post-hatch, a total of 336 Ross 308 male broilers were assigned to 6 dietary treatments with 8 replicate cages (7 birds/cage). Five experimental diets were formulated to incorporate non-extruded corn, extruded corn, wheat, wheat flour, and barley as the sole source of AA and energy.</p><p><strong>Results: </strong>Retention of dry matter and nitrogen, and energy concentrations in cereal grains determined by the total collection method were greater (p<0.05) than those determined by the index method. Energy concentrations of non-extruded and extruded corn were greater (p<0.05) than those of wheat, wheat flour, and barley. Wheat flour exhibited greater (p<0.05) ileal AA digestibility than non-extruded and extruded corn. Extruded corn and wheat showed comparable ileal AA digestibility values, whereas barley had the lowest among cereal grains.</p><p><strong>Conclusion: </strong>Energy concentrations of cereal grains determined by the total collection method were greater than those determined by the index method. Energy concentrations of non-extruded and extruded corn were greater compared to wheat, wheat flour, and barley, irrespective of the method used. The ileal AA digestibility in wheat flour was the greatest, followed by non-extruded corn, extruded corn, wheat, and barley in broilers.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"511-521"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of treated dried cassava stem replacement on feed intake, digestibility, rumen fermentation, and blood metabolites of Thai native cattle.","authors":"Sokchea Vong, Thearak Yi, Savdy Net, Sophany Morm, Areerat Lunpha, Chittraporn Yeanpet, Ruangyote Pilajun","doi":"10.5713/ab.24.0577","DOIUrl":"10.5713/ab.24.0577","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the use of cassava stems as an alternative feedstuff for ruminants, a study was conducted measuring the effect of replacing rice straw with untreated and treated dried cassava stems. The study assessed its impact on feed intake, nutrient digestibility, blood metabolites, and the growth performance of Thai native cattle.</p><p><strong>Methods: </strong>Six male cattle were arranged in a 3×3 replicated Latin square design to receive three treatments. All animals were provided with rice straw ad libitum and 14% crude protein (CP) concentrate at 0.5% body weight. Treatment variations consisted of a control group (Ctrl), one group that replaced rice straw with dried cassava stem (DCS), and another group that replaced rice straw with alkali, urea, and Ca(OH)2, treated dried cassava stem (tDCS) at 15% of rice straw intake. The experiment spanned three 21-day periods.</p><p><strong>Results: </strong>Feed intake, body weight gain, and feed conversion ratio (FCR) were similar across treatments. Total tract apparent digestibility for the cattle, which included dry matter, organic matter, CP, neutral detergent fiber, and acid detergent fiber, was higher than those on the tDCS treatment compared to the other groups (p<0.05). Ruminal pH, volatile fatty acid concentration, and blood metabolites remained unaffected by dietary treatments, except for BUN which was increased with tDCS replaced rice straw (p<0.05). Alkali-tDCS helped reduce rectum temperature compared to the control and DCS group (p<0.05).</p><p><strong>Conclusion: </strong>Replacing rice straw with tDCS improved nutrient digestibility but did not significantly impact feed intake, growth rate, FCR, rumen fermentation, and the blood metabolite of Thai native cattle.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"501-510"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}