Animal Bioscience最新文献

{"title":"Growth performance of pigs fed low-protein diets supplemented with crystalline amino acids at different growth stages.","authors":"Inho Cho, Changsu Kong","doi":"10.5713/ab.24.0339","DOIUrl":"10.5713/ab.24.0339","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the impact of reducing dietary crude protein (CP) coupled with supplementation of indispensable amino acids (AA) on growth performance of pigs at different growth stages.</p><p><strong>Methods: </strong>A total of 126 (63 barrows and 63 gilts), 90 (45 barrows and gilts), and 72 (36 barrows and 36 gilts) pigs with average weights of 9.8±1.62, 30.6±2.31, and 58.3±2.95 kg in the nursery, growing, and finishing stages, respectively, were assigned to three dietary treatments with six replicates in a randomized complete block design. The pigs had ad libitum access to water and fed three experimental diets, each supplemented with all indispensable AA and subjected to a 2% reduction in CP from the upper limits of 18%, 16%, and 16% established for the nursery, growing, and finishing stages, respectively.</p><p><strong>Results: </strong>In the nursery stage, from 0 to 2 weeks, reducing dietary CP concentrations decreased average daily feed intake (ADFI; linear, p = 0.04). From 2 to 4 weeks, dietary CP reduction decreased average daily gain (ADG; linear, p<0.01; quadratic, p = 0.02), ADFI (linear, p = 0.04), and gain-to-feed ratio (G:F; linear, p = 0.01). From 0 to 4 weeks, reduction in dietary CP concentrations decreased ADG (linear p<0.01), and G:F (linear, p = 0.01). In the growing stage, the dietary CP reduction did not affect growth performance. During the finishing stage, decrease in dietary CP concentrations decreased ADFI from 3 to 6 weeks (quadratic, p<0.01) and 0 to 6 weeks (quadratic, p = 0.01).</p><p><strong>Conclusion: </strong>Dietary CP reduction with indispensable AA supplementation potentially decreases the growth performance of nursery pigs but may not decrease the growth performance of growing and finishing pigs.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"316-324"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced aggregation of the leghorn male hepatoma cell line in suspension by supplementing dextran sulfate in the media.","authors":"Jung Soo Lim, Ji Soo Kim, Yugyeong Cheon, Tae Sub Park, Jong Kwang Hong","doi":"10.5713/ab.24.0372","DOIUrl":"10.5713/ab.24.0372","url":null,"abstract":"<p><strong>Objective: </strong>The study aimed to improve the efficiency of leghorn male hepatoma (LMH) cells for animal virus vaccine production by transitioning from adherent to suspension culture and evaluating the effects of dextran sulfate (DS) on preventing cell aggregation. The goal was to enhance cell growth, viability, and glucose metabolism and to develop efficient suspension-adapted LMH cells for large-scale vaccine production.</p><p><strong>Methods: </strong>LMH cells previously cultured in an adherent state were transferred to 125 mL Erlenmeyer flasks to conduct suspension culture. Cell culture performance, including cell density, viability, and glucose metabolism, during the cultures was measured, along with an assessment of cell aggregation. Additionally, mRNA expression levels of genes associated with cell adhesion and apoptosis were monitored.</p><p><strong>Results: </strong>DS supplementation in suspension culture enhanced cell viability and growth, with higher cell densities and viabilities compared to control media. Additionally, DS supplementation reduced glucose consumption and waste production, indicating improved metabolic efficiency. DS also delayed cell aggregation, possibly by downregulating integrin expression and promoting anti-apoptotic gene expression. However, even after 2 months, cell aggregation persisted in both control and DS-supplemented cultures, suggesting further optimization is needed for LMH cell adaptation to suspension culture.</p><p><strong>Conclusion: </strong>DS supplementation in LMH cell suspension cultures led to notable improvements in cell growth, viability, and glucose metabolism, while also decreasing the cell aggregation.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"350-359"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding molecular mechanisms of vertebral number of variations on Mongolian sheep using candidate genes analysis.","authors":"Chimgee Purev, Huiguang Wu, Khosbayar Lkhagva, Odbayar Tumendemberel","doi":"10.5713/ab.24.0212","DOIUrl":"10.5713/ab.24.0212","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the genetic link between variations in vertebral number and meat production traits, such as body weight and body measurements (body length, body height, heart girth, and shin width) in Mongolian (Bayantsagaan) sheep. Additionally, we examined the association of single-nucleotide polymorphisms (SNPs) in candidate genes, particularly vertnin (VRTN), nuclear receptor subfamily 6, group A, member 1 (NR6A1), and synapse differentiation-inducing 1-like (SYNDIG1L), with vertebral number variations and their potential impact on meat production traits.</p><p><strong>Methods: </strong>The study involved 220 Bayantsagaan sheep from Bayantsagaan soum, Tov province, Mongolia, including 104 sheep with extra vertebrae group and 116 individuals with typical vertebral number as the control group. Morphological data, including body weight and body measurements, were collected, and genetic samples were obtained. The impact of vertebral number on morphological traits was estimated using a general linear model. The SNPs in the VRTN, NR6A1, and SYNDIG1L genes were sequenced, and their association with vertebral number was analyzed using one-way analysis of variance.</p><p><strong>Results: </strong>Bayantsagaan sheep with extra vertebrae were, on average, 4.45 kg heavier and exhibited higher variability in body size traits compared to the control group. Four polymorphic sites were identified at the VRTN gene, with one polymorphic locus (VRTN1716) showing a significant association with vertebrae number and body size. Sheep with C/C genotype at VRTN1716 locus, had more vertebrae and larger body size compared to other genotypes.</p><p><strong>Conclusion: </strong>The findings suggest that variations in vertebral number and VRTN gene polymorphisms are linked to favorable meat production traits in Bayantsagaan sheep. The identified SNP (VRTN1716) associated with vertebral number and body size offers the potential for marker-assisted selection in breeding programs. These results provide valuable insights into the genetic basis of meat production traits in Bayantsagaan sheep and may contribute to the development of more efficient breeding strategies.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"247-254"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725747/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feeding levels during early gestation in a group-housing system for primiparous sows: impact on piglet birthweight and litter uniformity.","authors":"Natchanon Dumniem, Thomas D Parsons, Padet Tummaruk","doi":"10.5713/ab.24.0415","DOIUrl":"10.5713/ab.24.0415","url":null,"abstract":"<p><strong>Objective: </strong>The current study investigated the impacts of different feeding regimes during early gestation on conception rate, litter traits, piglet birthweight, and litter uniformity in primiparous sows.</p><p><strong>Methods: </strong>In total, 108 primiparous sows were inseminated and assigned to either a standard (1.9±0.5 kg/d, S) or high (2.9±0.8 kg/d, H) feeding levels during the first 35 days of gestation. The feeding regimes were categorized based on periods of gestation: 1 to 3, 4 to 15, and 16 to 35 days, resulting in four groups: standard-standard-standard (SSS, n = 26), standardstandard-high (SSH, n = 28), standard-high-high (SHH, n = 28), and high-high-high (HHH, n = 26). Afterwards, sows were placed into a group-housed system equipped with electronic sow feeders. The sows were weighed and assessed for backfat thickness and loin muscle depth at 0 and 35 days of gestation. At farrowing, data were collected on the total number of piglets born per litter, piglet birthweights, and the coefficient of variation of piglet birthweights.</p><p><strong>Results: </strong>On average, sows gained 22.5±21.6 kg during the first 35 days of gestation, showing a positive correlation with backfat gain (r = 0.954; p = 0.006). The backfat gain in the HHH group was higher than in the SSS (p = 0.016) and the SSH groups (p = 0.023), but did not differ from the SHH group (p = 0.684). Conception rates did not show differences among the feeding regimes (p>0.05). Individual piglet birthweights in the HHH group were higher than those in the SSH group (p<0.001). Likewise, the percentage of piglets with birthweights <1,000 g in the HHH group was lower than that in the SSH group (p<0.001). However, the variation of piglet birthweight did not differ among the groups (p>0.05).</p><p><strong>Conclusion: </strong>Increasing feeding levels in primiparous sows in a group-housed system during early pregnancy can effectively restore their body condition without any detrimental effects on subsequent litters.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"360-370"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New management grading for pig farms: management grading system using pig carcass weight, back fat thickness and k-means algorithm.","authors":"Youngho Lim, Jaeyoung Kim, Gwantae Kim, Jungseok Choi","doi":"10.5713/ab.24.0350","DOIUrl":"10.5713/ab.24.0350","url":null,"abstract":"<p><strong>Objective: </strong>This study categorized farm management levels to improve the productivity and uniformity of pork from pigs shipped from farms.</p><p><strong>Methods: </strong>A total of 48,298 pigs were grouped (A, B, C, D group) using the k-means algorithm, carcass weight and backfat thickness. The results of the grouping were used to classify Farm Management Grades (A, B, C, D grade).</p><p><strong>Results: </strong>The proportion of primal cuts in pigs, according to the new classification method, increased from group A to group D for shoulder blade, shoulder picnic, and ham, but decreased for loin and belly. In the regression analysis of the five primal cuts (shoulder blade, shoulder picnic, loin, belly, and ham) production (kg) for each group, all regression equations showed low errors (MAE<0.7), indicating that the model can predict the production of primal cuts by group. As the Farm Management Grade decreased, the proportion of pigs in the group with large differences from the mean of carcass weight and backfat thickness of the whole pig increased.</p><p><strong>Conclusion: </strong>The results of this study confirmed the differences in primal cut traits by pig grouping and created a method to classify farms who ship non-uniform pigs. This is expected to provide indicators for improvement and supplementation to farms that ship uneven pigs, helping to enhance the production of standardized pigs at the farm level.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"371-380"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of corn straw-based fermented total mixed rations supplemented with exogenous cellulase on growth performance, digestibility, and rumen fermentation in growing beef cattle.","authors":"Junzhao Xu, Xiaoni Wang, Huaxin Niu","doi":"10.5713/ab.24.0246","DOIUrl":"10.5713/ab.24.0246","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to assess the impact of corn straw-based unfermented and fermented total mixed rations (TMR) supplemented with exogenous cellulase on the in vitro fermentation characteristics, growth performance, feeding behavior, apparent digestibility, rumen fermentation and digestive enzyme activities of Chinese Simmental bulls.</p><p><strong>Methods: </strong>Unfermented (direct spraying of exogenous cellulase onto TMR, TMR) and fermented (exogenous cellulase fermentation for more than 7 d, fermented total mixed rations [FTMR]) TMR were collected, dried, powdered and used as fermentation substrates. The fermentation liquid was ruminal fluid collected from Chinese Simmental bulls. The artificial rumen culture fluid were continuously cultured in vitro for 48 h. Based on the diets they were fed, 24 healthy Chinese Simmental bulls (average weight of 495.93±10.89 kg) were randomly divided into two groups, with 12 bulls in each group, which were fed TMR or FTMR. The study lasted 56 d.</p><p><strong>Results: </strong>In in vitro experiments, the neutral detergent fiber (NDF) degradability and total volatile fatty acid, propionate, iso-butyrate, iso-valerate and valerate concentrations were greater in the FTMR group (p<0.05) than in the TMR group. However, the methane production, pH and acetate/propionate (A/P) of the FTMR group tended to be lower (p<0.05) than those of the TMR group. In the in vivo experiments, the average daily gain, eating rate, and feed efficiency of the FTMR groups were greater (p<0.05) than those of the TMR group. Similarly, the NDF degradability of the FTMR group was greater (p<0.05) than that of the TMR group. Compared to those in the TMR group, the concentrations of total volatile fatty acids, iso-butyrate, propionate and butyrate were greater in the FTMR group (p<0.05), and the A/P ratio was lower (p<0.05). Similarly, cellulase, xylanase, and β-glucosidase activities were greater (p<0.05) in the FTMR group than in the TMR group.</p><p><strong>Conclusion: </strong>Corn straw-based FTMR supplemented with exogenous cellulase play a vital role in decreasing the structural carbohydrate content of TMR and ruminal methane production in vitro, improving nutrient digestion and absorption, optimizing rumen fermentation, and improving the growth performance of beef cattle.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"293-302"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomal miR-222-3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting SOX10 in rabbits.","authors":"Yang Chen, Tingting Lu, Yingying Dai, Yu Xue, Bohao Zhao, Xinsheng Wu","doi":"10.5713/ab.24.0182","DOIUrl":"10.5713/ab.24.0182","url":null,"abstract":"<p><strong>Objective: </strong>Dermal papilla cells (DPCs) play a pivotal role in hair follicle development and can modulate melanogenesis in melanocytes (MCs) through their microenvironment. Our previous studies have demonstrated that the levels of exosomal miR-222-3p derived from DPCs of white Rex rabbits are significantly higher than those of black Rex rabbits. However, the specific role and underlying molecular mechanisms of exosomal miR-222-3p in melanogenesis remain elusive.</p><p><strong>Methods: </strong>DPCs and MCs were isolated from hair follicles of Rex rabbits and identified using western blotting (WB) and immunofluorescent staining. Exosomes derived from DPCs (DPCs-exos) were characterized using nanoparticle tracking analysis, transmission electron microscopy, and WB. To investigate cell-cell crosstalk mediated by exosomes, MCs were co-cultured with CM-Dil-labeled DPCs-exos. The expression of miR-222-3p in skin tissue and exosomes was quantitatively assessed using quantitative real-time polymerase chain reaction. The transmission of DPCs-secreted exosomal miR-222-3p to MCs was demonstrated using Cy3-labeled miR-222-3p in conjunction with transwell assays. The impact of miR-222-3p on melanin synthesis was evaluated using the NaOH method, cell counting kit-8, and annexin V-fluorescein isothiocyanate/propidium iodide assays. Sex determining region Y-box 10 (SOX10), a potential target gene regulated by miR-222-3p, was validated using a dual-luciferase reporter assay, site-specific mutation, and WB.</p><p><strong>Results: </strong>Increased levels of miR-222-3p were observed in the skin and DPCs-exos of white Rex rabbits compared to those of black Rex rabbits. Effective internalization of CM-Dillabeled DPCs-exos by MCs was observed. Furthermore, exosomal miR-222-3p derived from DPCs was transferred to MCs. Functionally, miR-222-3p significantly inhibited MCs proliferation, induced apoptosis and inhibited melanin synthesis. SOX10 was confirmed as a direct target of miR-222-3p in this regulatory cascade.</p><p><strong>Conclusion: </strong>The findings demonstrate that exosomal miR-222-3p, derived from DPCs, suppresses melanogenesis in MCs by targeting SOX10, thus unveiling a novel mechanism of exosome involvement in melanogenesis.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"236-246"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and phenotypic selection indices for decreased birth weight, shortening fattening period, and regulating total weight gain in beef cattle.","authors":"Kenji Togashi, Atsushi Ogino, Toshio Watanabe, Masakazu Shinomiya, Masashi Kinukawa, Kazuhito Kurogi, Masanobu Nurimoto","doi":"10.5713/ab.24.0171","DOIUrl":"10.5713/ab.24.0171","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to develop genomic and phenotypic indices for beef cattle selection that afford progeny with reduced birth weight and fattening period. The indices should also allow regulating total weight gain during the fattening period and avoid marked increases of inbreeding.</p><p><strong>Methods: </strong>Whether addition of constraint on total weight gain to constraints on body weight gain at up to four specific time points during the fattening process was effective for the weight gain until the end of the fattening period was examined in two selection indices with the selection trait being a phenotypic or a genomic breeding value random regression coefficient.</p><p><strong>Results: </strong>Both indices afforded cattle with desired weight gains at specific time points and desired total weight gains. One cycle of index-based selection made it possible to shorten the fattening period two weeks compared with that before selection while maintaining the same final fattening weight as before selection. The impact of constraining total weight gain was smallest when the number of weight gain constraints at specific time points was three or four. However, constraining total weight gain was necessary to avoid poor total weight gain when the number of weight gain constraints at specific time points was only one or two.</p><p><strong>Conclusion: </strong>The developed indices make it possible to regulate total weight gain during the fattening process and to achieve desired weight gains at specific time points. Importantly, the indices bring about genetic improvement without an excessive increase of inbreeding. Thus, we expect that these indices will contribute to sustainable genetic improvement in cattle while maintaining genetic diversity.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"223-235"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porcine granulosa cell transcriptomic analyses reveal the differential regulation of lncRNAs and mRNAs in response to all-trans retinoic acid in vitro.","authors":"Jinzhu Meng, Xiuwen Chen, Huabiao Wang, Yixuan Mi, Runsheng Zhou, Hongliang Zhang","doi":"10.5713/ab.24.0363","DOIUrl":"10.5713/ab.24.0363","url":null,"abstract":"<p><strong>Objective: </strong>The active metabolite of vitamin A, all-trans retinoic acid (ATRA), is involved in the proliferation and differentiation of granulosa cells, and promotes the follicular development, oocyte maturation, and ovulation in mammals. This study aims to investigate the ATRA induced potential long noncoding RNAs (lncRNAs) that regulate the expression of genes associated with granulosa cell proliferation and follicular development.</p><p><strong>Methods: </strong>The lncRNA and mRNA profiles of porcine granulosa cells from ATRA treatment and control group in vitro were constructed through RNA sequencing. Meanwhile, the sequencing data were verified using quantitative polymerase chain reaction (qPCR).</p><p><strong>Results: </strong>A total of 86 differentially expressed lncRNAs and 128 differentially expressed genes (DEGs) were detected in granulosa cells after ATRA treatment. The quantitative real-time PCR (qRT-PCR) results were consistent with the RNA-seq data. Functional annotation analysis revealed that the DEGs were remarkably enriched in ovary function and reproduction which contained FoxO, Hippo, Oocyte meiosis, mammalian target of rapamycin signaling pathway, as well as several pathways associated with hormone regulation like oxytocin signaling pathway and steroid hormone biosynthesis. Moreover, an interaction network of lncRNAs and their cis-target DEGs was constructed, and 7 differentially expressed lncRNAs and 6 cis-target DEGs were enriched in ovarian steroidogenesis and reproduction.</p><p><strong>Conclusion: </strong>These findings expand the lncRNA catalogue and provide a basis for further studies on the mechanism of ATRA-mediated lncRNA regulation of follicular development in pigs.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"267-277"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"shRNA-interfered of Nrf2 reveals a critical role for Keap1-Nrf2 signaling pathway during effects of zearalenone induced oxidative stress in IPEC-J2 cells.","authors":"Qun Cheng, Shu Zhen Jiang, Li Bo Huang, Wei Ren Yang","doi":"10.5713/ab.24.0368","DOIUrl":"10.5713/ab.24.0368","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to verify the protective effect of the Kelch-like ECH-associated protein1 (Keap1)-nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathways by studying the effect of plasmids containing Nrf2-small hairpin RNA (shRNA) interference down-regulation of Nrf2 on zearalenone (ZEA)-induced intestinal porcine epithelial cells (IPEC-J2) oxidative stress.</p><p><strong>Methods: </strong>We constructed an IPEC-J2 model that interferes with Nrf2 expression, set blank (control), negative control group (Sh-control), positive control group (Sh-Nrf2), and added 10, 20, and 40 μmol/L ZEA experimental group (Sh-Nrf2+ZEA10, Sh-Nrf2+ZEA20, and Sh-Nrf2+ZEA40).</p><p><strong>Results: </strong>The study results showed that, compared with the Sh-Nrf2 group, ZEA significantly increased the apoptosis rate of IPEC-J2 in a time- and dose-dependent manner. Compared with the Sh-Nrf2 group, the activities of total superoxide dismutase and glutathione peroxidase and relative expressions of Keap1 at mRNA and protein level in the Sh-Nrf2+ZEA20 and Sh-Nrf2+ZEA40 groups were significantly reduced, the malondialdehyde level, and the fluorescence intensity around and within the nucleus of reactive oxygen species and Nrf2, and the relative expressions of Nrf2, quinone oxidoreductase 1, and hemeoxygenase 1 at mRNA and protein level significantly increased.</p><p><strong>Conclusion: </strong>These results further prove that interfering with the expression of Nrf2 in IPEC-J2 cells affected the activation of the Keap1-Nrf2 signaling pathway and reduced the ability of cells to resist ZEA-induced oxidative stress. Therefore, the Keap1-Nrf2 signaling pathway had an important protective effect in ZEA-induced intestinal oxidative stress.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"303-315"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}