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Evaluation of feeding effects of pelletized total mixed ration in Hu sheep: growth performance, bacterial community and rumen fermentation. 评价颗粒状全混合日粮对湖羊的饲养效果:生长、瘤胃发酵和瘤胃细菌群落。
IF 2.5 2区 农林科学
Animal Bioscience Pub Date : 2025-10-01 Epub Date: 2025-05-19 DOI: 10.5713/ab.24.0852
Chuankai Zhang, Xiaohui Kong, Peijun Hou, Tengyun Gao, Huaijun Zheng, Wenqing Li, Tong Fu, Liyang Zhang
{"title":"Evaluation of feeding effects of pelletized total mixed ration in Hu sheep: growth performance, bacterial community and rumen fermentation.","authors":"Chuankai Zhang, Xiaohui Kong, Peijun Hou, Tengyun Gao, Huaijun Zheng, Wenqing Li, Tong Fu, Liyang Zhang","doi":"10.5713/ab.24.0852","DOIUrl":"10.5713/ab.24.0852","url":null,"abstract":"<p><strong>Objective: </strong>The study aimed to compare the growth performance, rumen fermentation, and rumen bacterial community of fattening Hu sheep fed either total mixed ration (TMR) or pelleted total mixed ration (PTMR) and to assess the feeding efficiency.</p><p><strong>Methods: </strong>In a 58-day feeding experiment, forty-eight Hu sheep were randomly assigned to two groups (TMR and PTMR), with six pens per group and four sheep per pen. Body weight and feed intake were measured throughout the experiment to assess growth performance. On the final day of the experiment, rumen fluid was collected from sheep using a rumen fluid collector two hours post-feeding.</p><p><strong>Results: </strong>The sheep in the PTMR group exhibited significantly higher body weight (p<0.05) and average daily gain (p<0.01) compared to those in the TMR group, although the effect of PTMR on dry matter intake was not statistically significant (p>0.05). No statistically significant differences were observed in rumen fermentation parameters between the TMR and PTMR groups. However, analysis of rumen bacteria revealed that the Sob, Ace, Bootstrap, Shannon, and Chao indices were significantly lower in the PTMR group compared to the TMR group (p<0.05). Bacteroidetes and Firmicutes were the predominant bacteria in all groups, with the abundance of Succinivibrionaceae_UCG-001 significantly lower in the PTMR group. The relative abundance of Rikenellaceae_RC9_gut_group, NK4A214_group, and Streptococcus was significantly higher in the TMR group than in the PTMR group (p<0.05). Bacterial function prediction showed downregulation of the energy production and conversion pathway in the PTMR group (p<0.05). Correlation analysis indicated that norank_f_Bacteroidales_RF16 positively correlated with butyric acid (p<0.05), while Anaerovibrio negatively correlated with acetic acid, propionic acid, butyric acid, and total volatile fatty acids (p<0.05).</p><p><strong>Conclusion: </strong>In summary, the results demonstrate that PTMR significantly enhances average daily gain in Hu sheep while maintaining rumen fermentation parameters, although accompanied by modifications in rumen bacterial community structure.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2125-2135"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bacillus subtilis: applications in the livestock and poultry industry in recent years: review. 枯草芽孢杆菌:近年来在畜禽工业中的应用。
IF 2.5 2区 农林科学
Animal Bioscience Pub Date : 2025-10-01 Epub Date: 2025-05-19 DOI: 10.5713/ab.25.0084
Xin Zhang, Ji Cao, Shi Zhe Han, Zhi Lin Liu, Ze Lin Jia, Jia Yu Cui, Yu Hang Zhang, Hui Sheng Xiong, Xue Li Wang
{"title":"Bacillus subtilis: applications in the livestock and poultry industry in recent years: review.","authors":"Xin Zhang, Ji Cao, Shi Zhe Han, Zhi Lin Liu, Ze Lin Jia, Jia Yu Cui, Yu Hang Zhang, Hui Sheng Xiong, Xue Li Wang","doi":"10.5713/ab.25.0084","DOIUrl":"10.5713/ab.25.0084","url":null,"abstract":"<p><p>Probiotics, as an alternative to antibiotics, are one of the better choices for ensuring product quality and food safety. Bacillus subtilis is often used in different forms, such as powder, granule, and liquid, in the livestock and poultry industry to produce feed and food additives and other products due to its advantages of high stability, low pathogenicity, high protein secretion capacity, and developed fermentation technology. This article reviews the biological characteristics, physiological functions, and effects on livestock and poultry production, as well as their mechanisms of action, to enhance the positive impact of the rational use of Bacillus subtilis as an additive in animal husbandry, such as feed and livestock food. In addition, this article also evaluates the potential of Bacillus subtilis to mitigate the negative effects of livestock and agricultural cultivation on the environment.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2043-2054"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Discovery and functional study of lncRNAs associated with fat deposition in Kele pigs based on whole-transcriptome RNA sequencing. 基于全转录组RNA测序的克勒猪脂肪沉积相关lncrna的发现和功能研究
IF 2.5 2区 农林科学
Animal Bioscience Pub Date : 2025-10-01 Epub Date: 2025-04-28 DOI: 10.5713/ab.24.0900
Xiong Zhang, Yong Guo, Zhonglong Zhao, Tiansong Wang, Qingshi Wang, Runqian Yang, Zhaobi Ai, Yong Zhang
{"title":"Discovery and functional study of lncRNAs associated with fat deposition in Kele pigs based on whole-transcriptome RNA sequencing.","authors":"Xiong Zhang, Yong Guo, Zhonglong Zhao, Tiansong Wang, Qingshi Wang, Runqian Yang, Zhaobi Ai, Yong Zhang","doi":"10.5713/ab.24.0900","DOIUrl":"10.5713/ab.24.0900","url":null,"abstract":"<p><strong>Objective: </strong>This study was conducted to identify lncRNAs associated with fat deposition in Kele pigs and investigate their molecular regulatory mechanisms.</p><p><strong>Methods: </strong>Six healthy 10-month-old male Kele pigs were selected as the experimental subjects and divided into high backfat thickness (>5%, H) and low backfat thickness group (<5%, L). Subcutaneous adipose tissue of each group was collected for whole-transcriptome RNA sequencing (RNA-seq). Subsequently, the biological functions of identified lncRNAs were investigated to explore their regulatory mechanisms on fat deposition in Kele pigs.</p><p><strong>Results: </strong>RNA-seq revealed 493 differentially expressed lncRNAs (p<0.05), with TCONS_00185937 and TCONS_00161198 implicated in fat deposition. Correlation analysis showed that the expression of TCONS_00185937 was significantly negatively correlated with backfat thickness, and the expression of TCONS_00161198 was significantly positively correlated with backfat thickness, and negatively correlated with eye muscle area. The pEGFP-TCONS_00185937 was transfected into subcutaneous fat precursor cells, it was found that the expression levels of TCONS_00185937 and its target genes MOGAT2 and ATGL were significantly higher than control group (p<0.05). Similarly, pEGFP-TCONS_00161198 transfected cells found that the expression levels of TCONS_00161198 and its target genes CSF3R and ACC were significantly higher than control group (p<0.05). The induced differentiation of transfected cells found that the OD510 absorption value of pEGFP-TCONS_00161198 group was higher than that of the control group. Triglyceride content was significantly higher than that of control group (p<0.01), while OD510 absorption value and triglyceride content of pEGFP-TCONS_00185937 group were lower than that of control group.</p><p><strong>Conclusion: </strong>The above results indicate that TCONS_00161198 and TCONS_00185937 may have regulatory effects on their target genes, thereby affecting fat deposition in pig subcutaneous fat precursor cells. This work provides a theoretical basis for the regulation of fat deposition in Kele pigs.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2079-2093"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential analysis of milk fatty acids in human, Saanen goat, Holstein cow, and Jersey cow milk at different stages of lactation. 人、萨宁奶山羊、中国荷斯坦奶牛和泽西奶牛不同泌乳期牛奶脂肪酸的差异分析。
IF 2.5 2区 农林科学
Animal Bioscience Pub Date : 2025-10-01 Epub Date: 2025-03-31 DOI: 10.5713/ab.24.0528
Yanni Wu, Xiang Cao, YuHao Wang, Rui Gao, Kun Wang, Yuan Yuan, YongJiang Mao, Xiang Chen, ZhangPing Yang, Zhi Chen
{"title":"Differential analysis of milk fatty acids in human, Saanen goat, Holstein cow, and Jersey cow milk at different stages of lactation.","authors":"Yanni Wu, Xiang Cao, YuHao Wang, Rui Gao, Kun Wang, Yuan Yuan, YongJiang Mao, Xiang Chen, ZhangPing Yang, Zhi Chen","doi":"10.5713/ab.24.0528","DOIUrl":"10.5713/ab.24.0528","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to investigate the differences in fatty acid composition of milk from Holstein cows, Jersey cows, Saanen goats, and humans at different lactation period and to find possible milk that is more suitable as a base for infant milk powder.</p><p><strong>Methods: </strong>Human breast milk, Saanen milk, Holstein milk, and Jersey milk were collected at different lactation stages. Gas chromatography was used to determine the fatty acid composition of breast milk, Saanen goat's milk, Holstein cow's milk and Jersey cows at different stages of lactation. Pearson correlation analysis was used to analyze the correlation between different fatty acids.</p><p><strong>Results: </strong>The results show that the types and relative contents of fatty acids varied among the different varieties of milk. The main fatty acids in breast milk are palmitic acid, oleic acid, and linoleic acid, which oleic acid is the most abundant. Saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) differed between lactation stages, while polyunsaturated fatty acids (PUFAs) did not differ significantly. Cow's milk and goat's milk were mainly dominated by SFAs, and Chinese Holstein cow's milk had the highest SFA content (72.54%). Caprylic and capric acids in SFAs were the characteristic fatty acids of goat's milk, and their contents were significantly higher than those of breast milk and cow's milk (p<0.05). The ratio of SFA:MUFA:PUFA breast milk was 1.72:1.45:1, Saanen goat's milk was 14.47:6:1, Chinese Holstein cow's milk was 14.98:4.84:1, and Jersey cow's milk was 13.32:4.47:1.</p><p><strong>Conclusion: </strong>None of the ruminant milk has the same proportion of fatty acids as breast milk, so none of them can completely replace breast milk. From the perspective of fatty acids, it is a better choice to choose Saanen goat milk or Jersey milk as a base and then add other ingredients to form infant milk powder.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2233-2249"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144061592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Use of cornstarch or fermented soybean meal in lactation diet improved sows' nutrient utilization and litter performance during lactation. 在泌乳期饲粮中添加玉米淀粉或发酵豆粕可提高母猪的养分利用率和产仔性能。
IF 2.5 2区 农林科学
Animal Bioscience Pub Date : 2025-10-01 Epub Date: 2025-06-10 DOI: 10.5713/ab.25.0106
Li Zhe, Hongmei Wen, Fangyuan Chen, Yong Zhuo, Yan Lin, Shengyu Xu, Xuemei Jiang, Lingjie Huang, Lianqiang Che, Bin Feng, De Wu, Takele Feyera, Zhengfeng Fang
{"title":"Use of cornstarch or fermented soybean meal in lactation diet improved sows' nutrient utilization and litter performance during lactation.","authors":"Li Zhe, Hongmei Wen, Fangyuan Chen, Yong Zhuo, Yan Lin, Shengyu Xu, Xuemei Jiang, Lingjie Huang, Lianqiang Che, Bin Feng, De Wu, Takele Feyera, Zhengfeng Fang","doi":"10.5713/ab.25.0106","DOIUrl":"10.5713/ab.25.0106","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the effects of cornstarch (CS) and fermented soybean meal (FSM) substitutions for conventional corn and soybean meal, respectively, on sow's performance, nutrient digestibility, milk composition, and oxidative status during lactation.</p><p><strong>Methods: </strong>Twenty-four lactating sows (8 sows/treatment) were assigned to either a standard lactation diet (CON), a CS diet in which pure CS substituted 60% of starch provided by conventional corn, or FSM diet in which FSM substituted 60% of crude protein provided by soybean meal. The experiment lasted from day 2 to 28 of lactation.</p><p><strong>Results: </strong>Compared with CON, the CS and FSM groups increased piglet live weight (p<0.05) and average daily gain (p<0.10), elevated milk superoxide dismutase and glutathione peroxidase (p<0.05), respectively, on day 28, and reduced (p<0.05) milk somatic cell counts on day 28; the CS group had higher (p<0.05) coefficient of apparent total tract digestibility of dry matter, gross energy, and ash but lower (p<0.05) serum β-hydroxybutyric acid and non-esterified fatty acids on day 28.</p><p><strong>Conclusion: </strong>In conclusion, the use of CS is much more robust in relieving body mobilization although both CS and FSM diets had beneficial effects on piglet performance by improving nutrient digestibility and milk quality of lactating sows.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2185-2195"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of dietary medium-chain fatty acid supplementation levels on growth performance, blood parameters, fecal score and metabolites in nursery pigs. 饲粮中链脂肪酸添加水平对苗猪生长性能、血液参数、粪便溃疡及代谢物的影响
IF 2.5 2区 农林科学
Animal Bioscience Pub Date : 2025-10-01 Epub Date: 2025-05-19 DOI: 10.5713/ab.25.0099
Chan Ho Kwon, Jannell A Torres, Madison J Mejia, Eva S Safaie, Joseph G Usack, Young Dal Jang
{"title":"Effects of dietary medium-chain fatty acid supplementation levels on growth performance, blood parameters, fecal score and metabolites in nursery pigs.","authors":"Chan Ho Kwon, Jannell A Torres, Madison J Mejia, Eva S Safaie, Joseph G Usack, Young Dal Jang","doi":"10.5713/ab.25.0099","DOIUrl":"10.5713/ab.25.0099","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the effects of increasing medium-chain fatty acid (MCFA) supplementation levels on growth performance, blood parameters, fecal score, and metabolites in nursery pigs.</p><p><strong>Methods: </strong>A total of 100 newly weaned pigs (initial body weight: 6.68±0.97 kg) were allotted to 5 treatments in 5 replicates, with 4 pigs per pen for a 28-d feeding trial. Treatments were MCFA supplementation levels at 0.0%, 0.4%, 0.8%, 1.2%, and 1.6%. The 2% soybean oil in the basal diet was replaced with MCFA (a 50:50 blend of free caproic and caprylic acids) on a weight-to-weight basis.</p><p><strong>Results: </strong>Increasing MCFA levels tended to linearly increase the final body weight (p = 0.06) and average daily gain in the overall period (p = 0.06), with no difference in overall average daily feed intake. The gain-to-feed ratio increased linearly in d 7-14 (p = 0.06, tendency) and 14-21 postweaning (p<0.05). Fecal score linearly decreased in d 0-7, 7-14, and 14-21 (p<0.05) postweaning, resulting in a linear reduction in the overall period (p<0.05). Plasma malondialdehyde levels at d 28 postweaning showed a quadratic decrease with increasing MCFA levels with the lowest value at 0.8% (p<0.05). Plasma free fatty acid levels at d 14 postweaning linearly decreased with increasing MCFA levels (p<0.05). Fecal total short-chain fatty acid (SCFA) concentrations tended to be lower (p = 0.09, tendency) in the MCFA treatments (0.4%-1.6%) than the control treatment (0.0%). There were no differences in plasma superoxide dismutase activity and fecal ammonia concentrations among dietary treatments.</p><p><strong>Conclusion: </strong>Increasing MCFA supplementation levels up to 1.6% could improve growth rate, feed efficiency, and fecal consistency without affecting feed intake and have the potential to reduce oxidative stress and free fatty acid levels in weaning pigs, while fecal SCFA concentrations could be slightly reduced by MCFA supplementation.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2173-2184"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploitation of pig by-product: physicochemical properties and flavor of Dachang Tang: effect of cooking time. 猪副产品的开发:大厂汤的理化性质及风味——蒸煮时间的影响。
IF 2.5 2区 农林科学
Animal Bioscience Pub Date : 2025-10-01 Epub Date: 2025-04-28 DOI: 10.5713/ab.24.0894
Tongxiang Yang, Jiayin Wei, Xiangtian Wu, Yunfei Wang, Kongyang Wu, Xu Duan, Wenchao Liu, Weiwei Cao, Linlin Li, Chung Lim Law, Junliang Chen, Guangyue Ren
{"title":"Exploitation of pig by-product: physicochemical properties and flavor of Dachang Tang: effect of cooking time.","authors":"Tongxiang Yang, Jiayin Wei, Xiangtian Wu, Yunfei Wang, Kongyang Wu, Xu Duan, Wenchao Liu, Weiwei Cao, Linlin Li, Chung Lim Law, Junliang Chen, Guangyue Ren","doi":"10.5713/ab.24.0894","DOIUrl":"10.5713/ab.24.0894","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to determine the optimal cooking time for Dachang Tang in order to promote Chinese cuisine culture and to improve the utilization of pig large intestines, thereby reducing resource waste.</p><p><strong>Methods: </strong>The raw pig large intestines were cleaned with water and flour, then boiled with selected spices for 40 mins to reduce off-odors. Subsequently, the large intestines were stir-fried with oil, garlic, and Zanthoxylum bungeanum for a specified duration, before being added to the original broth. The pig large intestines were then cooked for 10 mins, 20 mins, 40 mins, and 60 mins, respectively. Samples were collected at each cooking times to analyze their basic nutritional components (moisture, protein, fat), texture, color, water state, fatty acids, volatile compounds, microstructure, and sensory characteristics.</p><p><strong>Results: </strong>With the increase in cooking time, the moisture and fat content decreased significantly, and the ratio of unsaturated to saturated fatty acids increased (p<0.05). At 20 mins, pig large intestines exhibited optimal chewiness, color, surface integrity and sensory scores. Meanwhile, volatile flavor compounds mainly derived from spices, such as 4-methoxybenzaldehyde, benzaldehyde, 3-ethyltoluene, estragole, and ethyl decanoate effectively masking the off-flavor caused by p-cresol and indole in pig large intestines, and provided fruity, spicy, fennel and citrus aromas to Dachang Tang.</p><p><strong>Conclusion: </strong>A comprehensive analysis concluded that 20 mins was the optimal cooking time.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2266-2279"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vitamin C and N-acetylcysteine promote bovine adipose-derived mesenchymal stem cell proliferation and differentiation via Akt/mTOR/P70S6K signalling pathway for cultured meat production. 维生素C和n -乙酰半胱氨酸通过Akt/mTOR/P70S6K信号通路促进牛AD-MSCs的增殖和分化。
IF 2.5 2区 农林科学
Animal Bioscience Pub Date : 2025-10-01 Epub Date: 2025-05-19 DOI: 10.5713/ab.24.0776
Sajida Naseem, Mei-Fu Xuan, Huan Hua, Sungkwon Park, Abid Manzoor, Hailong Teng, Huaina Jin, Xiangzi Li, Qiang Li
{"title":"Vitamin C and N-acetylcysteine promote bovine adipose-derived mesenchymal stem cell proliferation and differentiation via Akt/mTOR/P70S6K signalling pathway for cultured meat production.","authors":"Sajida Naseem, Mei-Fu Xuan, Huan Hua, Sungkwon Park, Abid Manzoor, Hailong Teng, Huaina Jin, Xiangzi Li, Qiang Li","doi":"10.5713/ab.24.0776","DOIUrl":"10.5713/ab.24.0776","url":null,"abstract":"<p><strong>Objective: </strong>Traditional meat production is insufficient to meet the increasing protein requirements, necessitating cultured meat, which is safe, worthwhile, and scalable. Fat is essential for making cultured meat more acceptable to consumers by enhancing flavour and providing a natural appearance. Mesenchymal stem cells from adipose tissue are a promising source for this purpose, but in vitro expansion of cells decreases their proliferation ability and increases cellular senescence. The objective of this study was to improve the proliferation and differentiation abilities of adipose-derived mesenchymal stem cells (AD-MSCs).</p><p><strong>Methods: </strong>In this study, vitamin C (VC) and N-acetylcysteine (NAC) antioxidants were used to treat AD-MSCs from Yanbian cattle testicles. Cell counting kit, 5-ethynyl-2'-deoxyuridine staining, reverse transcription quantitative polymerase chain reaction, and western blot were used to test the cell viability and proliferation ability of AD-MSCs, ORO staining, triglycerides assay, and adipogenic specific markers expression were determined to analyse the adipogenic differentiation ability. Furthermore, oxidative stress parameters and activation of the Akt/mTOR/P70S6K signaling pathway were also studied.</p><p><strong>Results: </strong>Results showed that VC and NAC both increased proliferation and differentiation ability of AD-MSCs by increasing the expression of cell cycle regulatory and differentiation genes and proteins expression, and decreasing the expression of cell cycle inhibitory factors, and up-regulating stemness markers expression, while co-treatment showed enhanced effect. Oxidative stress was reduced by decreased reactive oxygen species production, malondialdehyde content, and enhanced glutathione activity, as well as declined cellular senescence. Subsequently, the Akt/mTOR/P70S6K signaling pathway was activated by VC and NAC+VC treatment in AD-MSCs, while NAC only activated Akt expression, indicating its role in controlled cell growth.</p><p><strong>Conclusion: </strong>This research concludes that NAC (2 mM) and VC (200 μM) improved the proliferation, differentiation potential, and stemness by decreasing oxidative stress and senescence, parallelly activating Akt/mTOR/P70S6K signaling pathway, while combined treatment (NAC+VC) enhanced these effects, providing bases for their utilisation to culture fat in cultivated meat production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2250-2265"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Estimating fecal output and nutrient digestibility using acid detergent insoluble ash as an intrinsic marker in lactating dairy cows: technical note. 用酸性洗涤不溶性灰分作为内在指标估计泌乳奶牛粪便排出量和营养物质消化率。
IF 2.5 2区 农林科学
Animal Bioscience Pub Date : 2025-10-01 Epub Date: 2025-05-12 DOI: 10.5713/ab.25.0130
Jungeun Kim, Kiyeon Park, Houhua Hu, Chanhee Lee
{"title":"Estimating fecal output and nutrient digestibility using acid detergent insoluble ash as an intrinsic marker in lactating dairy cows: technical note.","authors":"Jungeun Kim, Kiyeon Park, Houhua Hu, Chanhee Lee","doi":"10.5713/ab.25.0130","DOIUrl":"10.5713/ab.25.0130","url":null,"abstract":"<p><p>The current study was part of large experiments in a replicated 4×4 Latin square design where 4 dietary treatments (feed supplements) were examined. Fecal samples were obtained from the experiments via total collection (Experiment 1, n = 30; Experiment 2, n = 16). Feed and fecal samples were analyzed for acid detergent insoluble ash (ADIA) and nutrients, and those were used to estimate fecal output (FO) and nutrient digestibilites. Data within each experiment were analyzed using the Mixed procedure of SAS to determine diet, method (actual vs. estimated by ADIA), and their interaction on FO and apparent total tract digestibility of nutrients. A linear regression for FO between actual and estimated by ADIA was also conducted. The recovery of ADIA was 106% in both experiments. The use of ADIA tended to underestimate FO by 5% (Exp. 2, p = 0.09) and overestimate nutrient digestibilities by 2% to 5% (Exp. 1, p≤0.08). The linear regression resulted in the slope of 0.80 (against 1, p = 0.02) and the intercept of 1.93 (against 0, p = 0.21). In conclusion, ADIA is a potential marker for lactating cows in estimating FO when corrected by the factor obtained from the regression analysis.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2149-2154"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Policy recommendations for sustainable livestock farming in South Korea. 韩国可持续畜牧业的政策建议。
IF 2.5 2区 农林科学
Animal Bioscience Pub Date : 2025-09-30 DOI: 10.5713/ab.25.0307
Inkuk Yoon, Jong Hyun Jung, Sung Woo Kim, Sang Hyon Oh
{"title":"Policy recommendations for sustainable livestock farming in South Korea.","authors":"Inkuk Yoon, Jong Hyun Jung, Sung Woo Kim, Sang Hyon Oh","doi":"10.5713/ab.25.0307","DOIUrl":"https://doi.org/10.5713/ab.25.0307","url":null,"abstract":"<p><p>This study explores the transition towards sustainable livestock farming, emphasizing the role of policy instruments, challenges in implementation, and future directions. Major OECD member countries predominantly employ regulations, economic incentives, and promotional measures to promote sustainable livestock practices. Regulatory instruments, such as taxes and compliance obligations, are effective in addressing negative externalities, while economic incentives, like direct payment systems, are more accepted by stakeholders. Achieving policy effectiveness requires establishing consistent principles, ensuring stakeholder cooperation, and balancing regulatory and economic support. In South Korea, current policies include financial support for smart facilities, manure treatment, and eco-friendly products. However, there are gaps in greenhouse gas regulations and fragmented support measures. This study advocates comprehensive support systems and integrated manure management to address challenges like manure and odor management, which are critical for reducing greenhouse gas emissions. The integration of ICT technologies into livestock farming is highlighted as a means to improve management efficiency and competitiveness. Furthermore, the introduction of a \"Sustainable Livestock Public Subsidy System\" is proposed to encourage sustainable practices. This includes a certification system covering all production stages, ensuring products meet environmental and welfare standards. This study emphasizes the need for a decentralized subsidy management approach, tailored to regional conditions, to enhance policy effectiveness. It also addresses the importance of fostering a new workforce in the livestock sector, advocating for support for young and female farmers. Finally, the establishment of public-private governance is suggested to facilitate voluntary participation and consensus among stakeholders, ensuring a successful transition to sustainable livestock farming.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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