Animal Bioscience最新文献

{"title":"Effects of supplementing rumen-protected rubber seed oil to dairy cattle on feed digestibility and milk production.","authors":"Pongsatorn Gunun, Norakamol Laorodphan, Warachit Phayom, Walailuck Kaewwongsa, Chatchai Kaewpila, Waroon Khota, Nirawan Gunun","doi":"10.5713/ab.24.0287","DOIUrl":"10.5713/ab.24.0287","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to determine the effect of rumen-protected rubber seed oil (RPRSO) supplementation on feed digestibility, milk yield, and milk composition in tropical dairy cows.</p><p><strong>Methods: </strong>Twelve crossbred Holstien-Friesian dairy cows (75% Holstein-Friesian, 25% Thai native breed) with a mean body weight of 460±30 kg and 20±5 days in milk were randomly assigned to 1 of 3 treatments according to a completely randomized design. The treatments were as follows: a basal diet without rumen-protected fat (RPF) (control) or supplementation of rumen-protected palm oil (RPPO) at 300 g/h/d and RPRSO at 300 g/h/d. Each cow was fed a total mixed ration ad libitum.</p><p><strong>Results: </strong>The nutrient intake was similar among treatments (p>0.05). Adding RPF did not affect nutrient digestibility, while organic matter (OM) digestibility increased in dairy cows receiving RPRSO (p<0.01). Blood urea nitrogen (BUN), total protein, or glucose did not alter among treatments (p>0.05), while triglycerides and cholesterol were increased when cows were fed RPPO (p<0.01). Adding RPF increases milk yield in cows (p<0.01). The supplementation of RPRSO increased milk fat (p = 0.04). Milk fat yield was higher in RPPO and highest in RPRSO (p<0.01). The addition of RPF increased the oleic acid (OA, C18:1 cis-9) in milk (p = 0.01). In addition, cows fed RPRSO increased linoleic acid (LA; C18:2ω6 cis-9,12+trans-9,12) and α-linolenic acid (ALA; C18-3ω3 cis-9,12,15) in milk (p<0.01). The addition of RPF increased milk unsaturated fatty acids and monounsaturated fatty acids (p≤0.04). The polyunsaturated fatty acid (PUFA) in milk increased with RPRSO supplementation (p<0.01).</p><p><strong>Conclusion: </strong>Supplementation of RPRSO during early lactation can increase feed digestibility and the concentration of milk fat with PUFA (LA and ALA) in tropical dairy cows.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"665-672"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"- Invited Review - Challenges and constraints to the sustainability of poultry farming in China.","authors":"Fei Zhang, Zhuting Chen, Jiayi Shi, Chenglong Han, Qinyi Zhan, Zhouzheng Ren, Xiaojun Yang","doi":"10.5713/ab.24.0794","DOIUrl":"10.5713/ab.24.0794","url":null,"abstract":"<p><p>China's poultry industry is characterized by large-scale production and rich breeds, presenting both opportunities and challenges. In 2023, the industry produced 10.79 billion broilers, 28.38 million tons of eggs, and 4.88 billion waterfowl. The foundation of a thriving poultry industry lies in the continuous improvement of breeds. For instance, new lines of Lueyang black-boned chickens have been developed using genomic selection breeding, with a focus on improving production performance and unlocking their high-quality genetic potential. Precision nutrition programs enhance the expression of poultry's genetic potential and improve feed utilization efficiency. The five-dimensional feed evaluation system and the comprehensive National Feed Database provide formulators with accurate nutritional parameters of feed. Additionally, the concept of \"nutrition power\" and the \"five-ring gold standard\" enable researchers to analyze poultry's digestive physiology more effectively. Feeding management plays a crucial role in optimizing genetic potential and the effectiveness of precision nutrition. To further boost production efficiency, smart farming systems have been implemented, incorporating intelligent management of environmental factors, animal parameters, and poultry health tracking. Meanwhile, in order to improve material utilization efficiency across the entire poultry production chain and support the sustainable development of the poultry industry, it is essential to optimize and promote the application of the Poultry-Crop interaction systems. In summary, strengthening fundamental research in poultry, optimizing smart poultry farm platforms, and implementing Poultry-Crop Interacting systems will drive the sustainable development of China's poultry industry.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"789-801"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of fig and pineapple powder on metabolite compounds and health-promoting properties in fermented goat meat sausage.","authors":"Shine Htet Aung, Rupasinghe Ramesh Nimantha, Young-Sun Choi, Aera Jang, Jun Heon Lee, Ki-Chang Nam","doi":"10.5713/ab.24.0526","DOIUrl":"10.5713/ab.24.0526","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this research was to identify the metabolite compounds and health-promoting properties of goat meat fermented sausages containing different amounts of fig and pineapple powder (0.1%, 0.25%, and 0.5%), which are presented by F1, F2, F3 and P1, P2, P3, separately.</p><p><strong>Methods: </strong>Fermented sausages were manufactured from the lean meat of female goats. The samples extracted from the goat meat fermented sausages were evaluated for their metabolite compounds, antioxidants, and angiotensin-converting enzyme inhibitory activity.</p><p><strong>Results: </strong>The results showed that the diphenyl picrylhydrazyl radical scavenging activity, azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity, and angiotensin-converting enzyme inhibitory activity were the highest in F3 (p<0.05), whereas the hydroxyl radical scavenging capacity was the highest in P3 (p<0.05). In addition, F3 and F2 showed the highest superoxide anion radical scavenging activity (p<0.05), whereas F3 and P3 showed the highest copper chelating activity (p<0.05). Based on the metabolite compounds, hydrophobic amino acids, aromatic amino acids, and bitter amino acids were abundant in F3. Both F3 and P3 contained high concentrations of umami-producing compounds.</p><p><strong>Conclusion: </strong>The incorporation of 0.5% fig powder into goat meat fermented sausage showed improved biological activities based on metabolite compounds.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"752-764"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of host-specific and locally isolated multi-strain probiotics on piglet performance, mortality, inflammatory response, and gut microbiome.","authors":"Katatikarn Sahatsanon, Panneepa Sivapirunthep, Korawan Sringarm, Chaiwat Arjin, Patipan Hnokaew, Kamon Chaweewan, Chanporn Chaosap","doi":"10.5713/ab.24.0556","DOIUrl":"10.5713/ab.24.0556","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to assess the impact of host-specific and locally isolated multistrain probiotics on piglet performance, mortality, inflammatory responses, and gut microbiome.</p><p><strong>Methods: </strong>A total of 52 piglet litters-34 from Landrace sows and 18 from Large White sows-were allocated to two groups: a control group and a multi-strain probiotic group. The probiotic group comprised seven strains of lactic acid bacteria (MLAB): Lactobacillus brevis, Lactobacillus reuteri, Lactobacillus paraplantarum, Lactococcus lactis, Lactobacillus pentosus, Weissella cibaria, and Pediococcus pentosaceus. Each strain was included in equal concentrations, resulting in a final liquid mixture containing 109 colony forming units/mL. The MLAB group received the probiotics orally starting from 7 days of age until weaning at four weeks. Following weaning, supplementation continued via feed spraying for an additional four weeks.</p><p><strong>Results: </strong>MLAB supplementation did not significantly affect piglet performance but showed a trend towards reducing the mortality rate (p = 0.06). It influenced the inflammatory response by upregulating the expression of anti-inflammatory cytokines interleukin (IL)-4 and IL-10 (p<0.05). Microbial community analysis indicated that MLAB supplementation increased both microbial diversity (Simpson index: p = 0.06) and species richness (Chao1 index: p = 0.02). Piglets receiving MLAB had a significantly higher abundance of the phylum Firmicutes (p<0.01) compared to the control group, while the abundance of the phylum Bacteroidota was markedly reduced (p<0.01). In addition, the relative abundance of the bacterial genera Prevotellaceae_NK3B31 (p<0.01) and Chlamydia (p = 0.03) was lower in the MLAB group.</p><p><strong>Conclusion: </strong>Overall, these results suggest that while MLAB supplementation does not directly improve piglet growth performance, it has the potential to improve immune function and promote a healthier gut microbiota in weaning piglets, which could ultimately reduce mortality rates.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"717-727"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intermittent cold stimulation acclimates broilers to acute cold stress by affecting cardiac lipid metabolism.","authors":"Yong Zhang, Minghang Chang, Qiang Xue, Hongyu Wang, Yuanyuan Liu, Haidong Wei, Jianhong Li","doi":"10.5713/ab.24.0389","DOIUrl":"10.5713/ab.24.0389","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate whether intermittent cold stimulation can induce adaptation in broilers to acute cold stress (ACS) by regulating the lipid metabolism of hearts.</p><p><strong>Methods: </strong>CS0 were kept at normal rearing temperature, while CS3 and CS5 were exposed to 3°C for 3 and 5 hours, respectively, on alternate days lower than CS0 from 15d to 35d. On 50d, broilers in three groups were exposed to ACS at 10°C for 12 hours (Y12). The levels of corticosterone (CORT) and liothyronine (T3), mRNA and protein levels of heart adenosine monophosphate (AMP)-activated protein kinase/mammalian target of rapamycin (AMPK/mTOR) pathway genes were assessed at 36 d, 50 d and Y12.</p><p><strong>Results: </strong>At 36d, mRNA levels of AMPKα, acyl-CoA oxidase (ACO), mTOR, sterolregulatory element binding protein (SREBP), stearoyl-coA desaturase (SCD), acetyl-coA carboxylase (ACC), fatty acid synthase (FAS) and protein level of peroxisome proliferatorsactivated receptor α (PPARα) in CS3 and CS5 were significantly lower than those in CS0 (p<0.05). At 50d, compared to CS0, mRNA levels of PPARα, carnitine palmitoyltransferase1 (CPT1), ACO, tuberous sclerosis complex (TSC), SREBP and SCD, as well as protein levels of p-AMPKα/AMPKα, PPARα and SREBP were significantly increased in CS5 (p<0.05). At Y12, the levels of T3 in CS3 and CS5 were significantly higher than those in CS0 (p<0.05), mRNA levels of CPT1, ACO, SREBP, SCD and protein levels of p-AMPKα/AMPKα, SREBP, and FAS were significantly higher in CS5 than in CS0 and CS3 (p<0.05). However, compared to 50d, at Y12, mRNA levels of AMPKα, CPT1 and ACO in CS3 and CS5 significantly decreased (p<0.05), while protein levels of p-AMPKα/AMPKα significantly increased (p<0.05).</p><p><strong>Conclusion: </strong>This study suggested that intermittent cold stimulation at 3°C lower than normal rearing temperature for 5h could help broilers adapt to the ACS by promoting heart lipid metabolism.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"775-787"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rumen fermentation shifts and microbial dynamics in mid-lactating Holstein dairy cows experiencing heat stress and subsequent recovery periods.","authors":"Ye Pyae Naing, Seon-Ho Kim, A-Rang Son, Michelle Miguel, Joonpyo Oh, Sang-Suk Lee","doi":"10.5713/ab.24.0569","DOIUrl":"10.5713/ab.24.0569","url":null,"abstract":"<p><strong>Objective: </strong>In this study, we investigated the effects of heat stress (HS) on rumen fermentation, blood parameters, and ruminal microbial communities in mid-lactating Holstein dairy cows in Korea.</p><p><strong>Methods: </strong>Our study involved 12 mid-lactation Holstein dairy cows aged 55.54 months with 2.5±0.65 parities and 100 to 200 days in milking (DIM), fed a total mixed ratio diet. Samples were collected during HS (temperature-humidity index [THI] = 81.69) and recovery (RC) period (THI 69.84). The samples were analyzed for rumen fermentation, blood parameters, heat shock proteins, and microbial communities in dairy cows.</p><p><strong>Results: </strong>The milk yield, milk fat, milk protein, and milk urea nitrogen levels differed significantly between two-time points (p<0.05). Rumen pH and volatile fatty acid concentrations, the pH was not significantly different (p = 0.619) between HS and RC periods; however, the ammonia nitrogen (NH3-N) levels increased during HS period ), however, there was no significant difference (p>0.05). Blood total protein significantly increased during HS period compared with that during RC period (p<0.05), while no significant differences were observed in other parameters between the two periods. HSP27, HSP70, and HSP90 increased in dairy cows under HS conditions compared with those during the RC period. Taxonomic classification revealed that Firmicutes and Bacteroidetes dominated the bacterial community. PERMANOVA and PERMDISP showed significant differences in rumen bacterial diversity between HS and RC periods, based on Unifrac metrics (p = 0.044 and p = 0.015, respectively), indicating taxonomic variations. Microbial networks with correlations of >0.8 (p<0.05) showed a complex structure with equal positive and negative connections, indicating Anaerohabdus furcosa and Ruminiclostridium cellobioparum as key species during the HS and RC periods respectively.</p><p><strong>Conclusion: </strong>HS significantly impacts Holstein dairy cows' physiological and metabolic processes, altering rumen fermentation, blood biochemistry, and gut microbiota during mid-lactation.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"641-654"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of replacing soybean meal with processed soybean meal on intestinal health and growth of nursery pigs challenged with F18+ Escherichia coli.","authors":"Zixiao Deng, Hyunjun Choi, Sung Woo Kim","doi":"10.5713/ab.24.0566","DOIUrl":"10.5713/ab.24.0566","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the impact of different level of soybean meal (SBM) replaced by soy protein concentrate on intestinal health and growth performance of nursery pigs under F18+ Escherichia coli (E. coli).</p><p><strong>Methods: </strong>Forty-eight newly weaned pigs (6.6±0.3 kg) were randomly allotted to 4 treatments arranged by 2×2 factors using randomized complete block design with initial body weight and sex as blocks. Two factors were F18+ E. coli challenge (0 or 2.1×1010 colony-forming units [CFU]) and the level of SBM (24% or 12% in phase 1 and 26% or 14% in phase 2). Pigs were fed for 25 d in 2 phases (phase 1 for 11 d and phase 2 for 14 d). At the end of study, all pigs were euthanized to collect jejunal mucosa and tissues.</p><p><strong>Results: </strong>The F18+ E. coli challenge decreased (p<0.05) overall average daily gain (ADG) and average daily feed intake (ADFI) and decreased (p<0.05) gain to feed ratio on d 7 to 11. The high SBM tended to have a greater overall ADG (p = 0.054) and ADFI (p = 0.078) compared with low SBM under F18+ E. coli challenge, but not in unchallenged conditions. The F18+ E. coli challenge increased (p<0.05) fecal score on d 7 to 18. The tumor necrosis factor-α and interleukin-1β in jejunal mucosa were decreased (p<0.05) in high SBM treatments. The high SBM tended to increase (p = 0.085) occludin expression in jejunum. high SBM increased crypt depth in jejunum under F18+ E. coli challenge, but not in unchallenged conditions (p<0.05).</p><p><strong>Conclusion: </strong>High SBM in nursery diets could alleviate the detrimental effects of F18+ E. coli challenge on growth performance of pigs under compared to low SBM inclusion, which might be attributed to decreased intestinal inflammation and improved intestinal integrity.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"728-738"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of liver and ileum reveals potential regulation of long non-coding RNA in pigs with divergent feed efficiency.","authors":"Zhenjian Zhao, Shujie Wang, Kai Wang, Xiang Ji, Dong Chen, Qi Shen, Yang Yu, Shendi Cui, Junge Wang, Ziyang Chen, Guoqing Tang","doi":"10.5713/ab.24.0434","DOIUrl":"10.5713/ab.24.0434","url":null,"abstract":"<p><strong>Objective: </strong>Long non-coding RNA (LncRNA) plays a significant role in regulating feed efficiency. This study aims to explore the key lncRNAs, associated genes, and pathways in pigs with extreme feed efficiencies.</p><p><strong>Methods: </strong>We screened pigs with extremely high and low residual feed intake through a 12-week animal growth trial and then conducted transcriptome analysis on their liver and ileum tissues. We analyzed the differential expressed lncRNAs, microRNAs (miRNAs), and messenger RNAs through target gene prediction and functional analysis. And we identified key lncRNAs and their potential regulatory genes associated with feed efficiency through the construction of competitive endogenous RNA network.</p><p><strong>Results: </strong>Differentially expressed lncRNAs were pinpointed in the liver, revealing 23 crucial target genes primarily associated with guanosine triphosphate metabolism and glycolipid biosynthesis. In the ileum, a screening identified 92 pivotal target genes, mainly linked to lipid and small molecule metabolism. Moreover, LOC106504303 and LOC102160805 emerged as potentially significant lncRNAs respectively, playing roles in mitochondrial oxidative phosphorylation in the liver, and lipid and cholesterol metabolism in the ileum.</p><p><strong>Conclusion: </strong>The lncRNAs regulate energy metabolism and biosynthesis in the liver, and the digestive absorption capacity in the small intestine, affecting the feed efficiency of pigs.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"588-599"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chi-circ_0009659 modulates goat intramuscular adipocyte differentiation through miR-3431-5p/STEAP4 axis.","authors":"Xin Li, Hao Zhang, Yong Wang, Yanyan Li, Youli Wang, Yan Xiong, Wei Liu, Yaqiu Lin","doi":"10.5713/ab.24.0322","DOIUrl":"10.5713/ab.24.0322","url":null,"abstract":"<p><strong>Objective: </strong>Circular RNAs (circRNAs) are widely involved in the regulation of lipid deposition in animals, but there are few reports on key circRNAs regulating intramuscular adipocyte differentiation in goats. Therefore, this study took an abundantly expressed in goat adipocytes chi-circ_0009659 as the object.</p><p><strong>Methods: </strong>Based on the identification of back splicing site in chi-circ_0009659, its expression level during the goat intramuscular preadipocyte differentiation was detected by quantitative polymerase chain reaction (qPCR) . The chi-circ_0009659 loss-of-function and gain-of-function cell models were obtained by adenovirus and smarter silencer, respectively. and the adipocyte differentiation were explored by Oil Red O staining, Bodipy staining and qPCR. Its major cytoplasmic localization was determined by fluorescence in situ hybridization (FISH), nucleocytoplasmic separation and qPCR. The interaction between chi-circ_0009659, miR-3431-5p, and STEAP family member 4 (STEAP4) was verified by bioinformatics, RNA pull down and dual luciferase reporter assay.</p><p><strong>Results: </strong>Silencing chi-circ_0009659 inhibited lipid droplet accumulation and the expression of differentiation-determining genes in goat intramuscular adipocytes, while overexpression of chi-circ_0009659 reversed these results. chi-circ_0009659 was predominantly localized to the cytoplasm and could regulate miR-3431 expression which in turn affects STEAP4. Consistent with expectations, miR-3431-5p acted as a negative regulator of GIMPA differentiation, while STEAP4 promoted differentiation.</p><p><strong>Conclusion: </strong>We demonstrated chi-circ_0009659 positively regulates goat intramuscular preadipocyte differentiation by sponging miR-3431-5p to further regulate the expression of STEAP4. This research provides a new reference for in-depth understanding of the effects of circRNA on adipocyte differentiation.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"577-587"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intracellular domain of CATSPER1 could serve as a cytoplasmic platform for redox processes in mammalian sperm.","authors":"Jingon Kim, Jae Yeon Hwang","doi":"10.5713/ab.24.0631","DOIUrl":"10.5713/ab.24.0631","url":null,"abstract":"<p><strong>Objective: </strong>Mammalian sperm acquire fertilizing ability in the female reproductive tract and develop hyperactivated motility, which is indispensable for male fertility. Hyperactivated motility is initiated by Ca2+ influx via the sperm-specific ion channel, CatSper. CATSPER1, a CatSper pore subunit, possesses a long N-terminal intracellular domain and its degradation correlates with unsuccessful sperm migration in the female tract. However, the cellular function and molecular significance of the CATSPER1 N-terminal domain are not well understood. Here, we identify the interactome of the CATSPER1 N-terminal domain and propose a function for the intracellular domain in mammalian sperm.</p><p><strong>Methods: </strong>To identify CATSPER1 N-terminus interactome, we produced recombinant CATSPER1-N-terminus in bacterial system. The purified protein was incubated with testicular lysates and eluted together with testicular interacting proteins. The elutes were subjected to proteomic analysis and CATSPER1-N-terminus interactome was profiled. Identified proteins were further analyzed by functional annotation.</p><p><strong>Results: </strong>We purified the partial CATSPER1 N-terminal domain and identified 57 testicular proteins as domain interactomes using mass spectrometry analysis. Functional annotation analysis revealed that 106 gene ontologies were significantly enriched, 16 of which were related to redox processes. We found that antioxidant enzymes, such as PARK7 and PRDX2, 4, and 6, were included in the enriched redox-related gene ontologies.</p><p><strong>Conclusion: </strong>These results suggest that the CATSPER1 N-terminus could function in defending against oxidative stress to support the successful migration of mammalian sperm to fertilizing sites in the female reproductive tract.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"655-664"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}