Phuphan chicken breeds: classification as varieties or distinct breeds with three derivative groups using microsatellite genotyping.

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Bioscience Pub Date : 2025-10-01 Epub Date: 2025-05-19 DOI:10.5713/ab.24.0579
Ekerette Ekerette, Nivit Tanglertpaibul, Trifan Budi, Wisut Auekingpetch, Chien Phuoc Tran Nguyen, Worapong Singchat, Wongsathit Wongloet, Nichakorn Kumnan, Piangjai Chalermwong, Anh Huynh Luu, Thitipong Panthum, Aingorn Chaiyes, Kanithaporn Vangnai, Chotika Yokthongwattana, Chomdao Sinthuvanich, Narongrit Muangmai, Prateep Duengkae, Kornsorn Srikulnath
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引用次数: 0

Abstract

Objective: Indigenous and local breeds, such as Phuphan chickens, are vital due to their adaptability and nutritional value. However, the precise origin, historical records, and genetic diversity of Phuphan chickens remain unclear. This study aimed to evaluate origin and genetic diversity of four Phuphan chicken groups from the Phuphan Royal Development Study Centre.

Methods: This study assesses four groups of Phuphan chicken: Phuphan black 1 (SK-B1), Phuphan black 2 (KU-BM/F), Phuphan white (KU-WM/F), and Phuphan color (KU-VM/F) using 28 microsatellite markers and comparing them with those of other Thai chicken breeds within "The Siam Chicken Bioresource Project" database.

Results: The results highlighted significant genetic diversity among these groups (mean expected heterozygosity [He] = 0.623±0.014; Allelic richness [AR] = 4.594±0.124), indicating effective management through the breeding program of the Phuphan Royal Development Study Centre. Population structure analyses revealed distinct gene pools, emphasizing the genetic uniqueness of SK-B1 relative to the other three groups. Bayesian inference validated historical genetic exchanges, primarily among KU-BM/F, KU-WM/F, and KU-VM/F, with limited exchanges involving SK-B1. This suggests that the Phuphan chicken groups share a common lineage, primarily distinguished by variations in plumage color, resulting from residual selection processes. Microsatellite markers pinpointed the loci LEI0234, MCW206, MCW0016, MCW0222, MCW0098, MCW0165, and ADL0278 as potentially subject to directional selection and associated with plumage color variation among the Phuphan chicken groups. Comparative evaluations with other Thai indigenous local chickens and red junglefowl revealed a closer affinity of SK-B1 to existing Thai chicken breeds, suggesting it may represent a variant of these breeds. Alternatively, KU-BM/F, KU-WM/F, and KU-VM/F, which exhibited comparable external characteristics, may constitute a novel breed of Phuphan chicken.

Conclusion: The findings may enhance understanding on genetic architecture of Phuphan chicken groups and contribute to Thailand's economic growth while preserving the genetic diversity of the indigenous chickens.

phphan鸡品种:用微卫星基因分型技术将其分类为品种或不同品种。
目标:土著和地方品种,如phphan鸡,因其适应性和营养成分而至关重要。然而,phphan鸡的确切起源、历史记录和遗传多样性仍不清楚。因此,本研究旨在评估来自Phuphan皇家发展研究中心的四个Phuphan鸡群的起源和遗传多样性。方法:利用28个微卫星标记对phphan黑1 (SK-B1)、phphan黑2 (KU-BM/F)、phphan白(KU-WM/F)和phphan彩色(KU-VM/F) 4个phphan鸡类群进行评价,并与“暹罗鸡生物资源项目”数据库中的其他泰国鸡品种进行比较。结果:各组间遗传多样性显著(平均期望杂合度(He)=0.623±0.014;等位基因丰富度(AR)=4.594±0.124),表明通过Phuphan皇家发展研究中心的育种计划进行了有效的管理。通过群体结构分析确定了不同的基因库,强调SK-B1与其他三个群体相比具有遗传独特性。贝叶斯推断证实了历史遗传交换,主要发生在KU-BM/F、KU-WM/F和KU-VM/F之间,涉及SK-B1的交换很少。这表明phphan鸡群有一个共同的血统,主要通过羽毛颜色区分,通过残余选择过程。微卫星标记确定了基因座LEI0234、MCW206、MCW0016、MCW0222、MCW0098、MCW0165和ADL0278可能存在定向选择,并与phphan鸡群体的羽毛颜色变化有关。与其他泰国本土鸡和地方鸡以及红丛林雉的比较评估显示,SK-B1与现有的泰国鸡品种有更密切的亲缘关系,表明它可能是泰国鸡品种的一个变体。另外,外观相似的KU-BM/F、KU-WM/F和KU-VM/F可能构成一个新的phphan鸡品种。结论:这些发现有助于提高对phphan鸡类群遗传结构的认识,在保护phphan鸡遗传多样性的同时促进泰国经济增长。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
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