基于全转录组RNA测序的克勒猪脂肪沉积相关lncrna的发现和功能研究

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Bioscience Pub Date : 2025-10-01 Epub Date: 2025-04-28 DOI:10.5713/ab.24.0900
Xiong Zhang, Yong Guo, Zhonglong Zhao, Tiansong Wang, Qingshi Wang, Runqian Yang, Zhaobi Ai, Yong Zhang
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引用次数: 0

摘要

目的:筛选克勒猪脂肪沉积相关lncrna,并通过生物学功能验证探索其分子调控机制,有望为克勒猪的育种、开发利用提供理论依据。方法:选取6头10月龄健康克勒猪作为实验对象,分为高、低背膘厚组。收集各组皮下脂肪组织进行全转录组RNA测序(RNA-seq),随机选取与背膘厚度相关的lncrna进行实时定量PCR (RT-qPCR)分析。进行lncRNAs表达与生产数据的相关性分析,鉴定lncRNAs的生物学功能。结果:通过RNA-seq共发现493个表达差异显著的lncrna (p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Discovery and functional study of lncRNAs associated with fat deposition in Kele pigs based on whole-transcriptome RNA sequencing.

Objective: This study was conducted to identify lncRNAs associated with fat deposition in Kele pigs and investigate their molecular regulatory mechanisms.

Methods: Six healthy 10-month-old male Kele pigs were selected as the experimental subjects and divided into high backfat thickness (>5%, H) and low backfat thickness group (<5%, L). Subcutaneous adipose tissue of each group was collected for whole-transcriptome RNA sequencing (RNA-seq). Subsequently, the biological functions of identified lncRNAs were investigated to explore their regulatory mechanisms on fat deposition in Kele pigs.

Results: RNA-seq revealed 493 differentially expressed lncRNAs (p<0.05), with TCONS_00185937 and TCONS_00161198 implicated in fat deposition. Correlation analysis showed that the expression of TCONS_00185937 was significantly negatively correlated with backfat thickness, and the expression of TCONS_00161198 was significantly positively correlated with backfat thickness, and negatively correlated with eye muscle area. The pEGFP-TCONS_00185937 was transfected into subcutaneous fat precursor cells, it was found that the expression levels of TCONS_00185937 and its target genes MOGAT2 and ATGL were significantly higher than control group (p<0.05). Similarly, pEGFP-TCONS_00161198 transfected cells found that the expression levels of TCONS_00161198 and its target genes CSF3R and ACC were significantly higher than control group (p<0.05). The induced differentiation of transfected cells found that the OD510 absorption value of pEGFP-TCONS_00161198 group was higher than that of the control group. Triglyceride content was significantly higher than that of control group (p<0.01), while OD510 absorption value and triglyceride content of pEGFP-TCONS_00185937 group were lower than that of control group.

Conclusion: The above results indicate that TCONS_00161198 and TCONS_00185937 may have regulatory effects on their target genes, thereby affecting fat deposition in pig subcutaneous fat precursor cells. This work provides a theoretical basis for the regulation of fat deposition in Kele pigs.

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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
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