Discovery and functional study of lncRNAs associated with fat deposition in Kele pigs based on whole-transcriptome RNA sequencing.

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Bioscience Pub Date : 2025-10-01 Epub Date: 2025-04-28 DOI:10.5713/ab.24.0900
Xiong Zhang, Yong Guo, Zhonglong Zhao, Tiansong Wang, Qingshi Wang, Runqian Yang, Zhaobi Ai, Yong Zhang
{"title":"Discovery and functional study of lncRNAs associated with fat deposition in Kele pigs based on whole-transcriptome RNA sequencing.","authors":"Xiong Zhang, Yong Guo, Zhonglong Zhao, Tiansong Wang, Qingshi Wang, Runqian Yang, Zhaobi Ai, Yong Zhang","doi":"10.5713/ab.24.0900","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study was conducted to identify lncRNAs associated with fat deposition in Kele pigs and investigate their molecular regulatory mechanisms.</p><p><strong>Methods: </strong>Six healthy 10-month-old male Kele pigs were selected as the experimental subjects and divided into high backfat thickness (>5%, H) and low backfat thickness group (<5%, L). Subcutaneous adipose tissue of each group was collected for whole-transcriptome RNA sequencing (RNA-seq). Subsequently, the biological functions of identified lncRNAs were investigated to explore their regulatory mechanisms on fat deposition in Kele pigs.</p><p><strong>Results: </strong>RNA-seq revealed 493 differentially expressed lncRNAs (p<0.05), with TCONS_00185937 and TCONS_00161198 implicated in fat deposition. Correlation analysis showed that the expression of TCONS_00185937 was significantly negatively correlated with backfat thickness, and the expression of TCONS_00161198 was significantly positively correlated with backfat thickness, and negatively correlated with eye muscle area. The pEGFP-TCONS_00185937 was transfected into subcutaneous fat precursor cells, it was found that the expression levels of TCONS_00185937 and its target genes MOGAT2 and ATGL were significantly higher than control group (p<0.05). Similarly, pEGFP-TCONS_00161198 transfected cells found that the expression levels of TCONS_00161198 and its target genes CSF3R and ACC were significantly higher than control group (p<0.05). The induced differentiation of transfected cells found that the OD510 absorption value of pEGFP-TCONS_00161198 group was higher than that of the control group. Triglyceride content was significantly higher than that of control group (p<0.01), while OD510 absorption value and triglyceride content of pEGFP-TCONS_00185937 group were lower than that of control group.</p><p><strong>Conclusion: </strong>The above results indicate that TCONS_00161198 and TCONS_00185937 may have regulatory effects on their target genes, thereby affecting fat deposition in pig subcutaneous fat precursor cells. This work provides a theoretical basis for the regulation of fat deposition in Kele pigs.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"2079-2093"},"PeriodicalIF":2.5000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415382/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Bioscience","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.5713/ab.24.0900","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/28 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0

Abstract

Objective: This study was conducted to identify lncRNAs associated with fat deposition in Kele pigs and investigate their molecular regulatory mechanisms.

Methods: Six healthy 10-month-old male Kele pigs were selected as the experimental subjects and divided into high backfat thickness (>5%, H) and low backfat thickness group (<5%, L). Subcutaneous adipose tissue of each group was collected for whole-transcriptome RNA sequencing (RNA-seq). Subsequently, the biological functions of identified lncRNAs were investigated to explore their regulatory mechanisms on fat deposition in Kele pigs.

Results: RNA-seq revealed 493 differentially expressed lncRNAs (p<0.05), with TCONS_00185937 and TCONS_00161198 implicated in fat deposition. Correlation analysis showed that the expression of TCONS_00185937 was significantly negatively correlated with backfat thickness, and the expression of TCONS_00161198 was significantly positively correlated with backfat thickness, and negatively correlated with eye muscle area. The pEGFP-TCONS_00185937 was transfected into subcutaneous fat precursor cells, it was found that the expression levels of TCONS_00185937 and its target genes MOGAT2 and ATGL were significantly higher than control group (p<0.05). Similarly, pEGFP-TCONS_00161198 transfected cells found that the expression levels of TCONS_00161198 and its target genes CSF3R and ACC were significantly higher than control group (p<0.05). The induced differentiation of transfected cells found that the OD510 absorption value of pEGFP-TCONS_00161198 group was higher than that of the control group. Triglyceride content was significantly higher than that of control group (p<0.01), while OD510 absorption value and triglyceride content of pEGFP-TCONS_00185937 group were lower than that of control group.

Conclusion: The above results indicate that TCONS_00161198 and TCONS_00185937 may have regulatory effects on their target genes, thereby affecting fat deposition in pig subcutaneous fat precursor cells. This work provides a theoretical basis for the regulation of fat deposition in Kele pigs.

基于全转录组RNA测序的克勒猪脂肪沉积相关lncrna的发现和功能研究
目的:筛选克勒猪脂肪沉积相关lncrna,并通过生物学功能验证探索其分子调控机制,有望为克勒猪的育种、开发利用提供理论依据。方法:选取6头10月龄健康克勒猪作为实验对象,分为高、低背膘厚组。收集各组皮下脂肪组织进行全转录组RNA测序(RNA-seq),随机选取与背膘厚度相关的lncrna进行实时定量PCR (RT-qPCR)分析。进行lncRNAs表达与生产数据的相关性分析,鉴定lncRNAs的生物学功能。结果:通过RNA-seq共发现493个表达差异显著的lncrna (p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信