{"title":"饲粮中添加单宁酸对浏阳黑山羊甲烷产量、瘤胃发酵、瘤胃菌群及营养物质消化的影响","authors":"Ying Yun, Ting Liu, Hui Hui Liu, Hui Zhang, Faming Pan, Lijing An, Xinji Wang, Guoyan Xu, Qiangwen Gu, Chen Zheng","doi":"10.5713/ab.25.0114","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the effects of dietary tannic acid on methane production, nutrient digestibility, rumen fermentation, and rumen microbiota in Liuyang black goats.</p><p><strong>Methods: </strong>Twelve adult goats were randomly assigned to two groups: a control group (CON) and a treatment group (TAN) that received 2 % tannic acid in their diet. The experiment had two stages, each with a 10 - day adaptation and a 5 - day sampling phase. Methane emission was measured using a mobile open-circuit respirometry system, while rumen fluid samples were analyzed for volatile fatty acids (VFAs), ammonia nitrogen, and microbial composition via 16S rRNA sequencing.</p><p><strong>Results: </strong>The results indicated that the tannic acid significantly reduced overall methane emission (p < 0.05), methane per voluntary feed intake (VFI, p < 0.05), dry matter intake (DMI, p < 0.05), acid detergent fiber intake (ADFI, p < 0.05), and neutral detergent fiber intake (NDFI, p < 0.05). Microbial analysis showed increased relative abundance of Firmicutes (p < 0.05) and decreased Methanobrevibacter and Prevotella. Tannic acid also improved ADF digestibility (p < 0.05) and altered VFA profiles by increasing acetic acid to propionic acid ratios and valeric and butyric acids (p < 0.05). Tannic acid supplementation also increases the ratios of acetic acid to propionic acid, as well as valeric and butyric acids (p < 0.05), while increasing the digestibility of ADF (p < 0.05).</p><p><strong>Conclusion: </strong>Dietary tannic acid effectively reduced methane emission and enhanced feed efficiency in Liuyang black goats by modifying rumen fermentation and microbial activity. These findings suggest tannic acid's potential as a sustainable feed additive for ruminants, although long-term impacts on health and productivity require further investigation.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of dietary supplementing tannic acid on methane production, rumen fermentation, rumen microflora and nutrient digestion of Liuyang black goats.\",\"authors\":\"Ying Yun, Ting Liu, Hui Hui Liu, Hui Zhang, Faming Pan, Lijing An, Xinji Wang, Guoyan Xu, Qiangwen Gu, Chen Zheng\",\"doi\":\"10.5713/ab.25.0114\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>This study investigated the effects of dietary tannic acid on methane production, nutrient digestibility, rumen fermentation, and rumen microbiota in Liuyang black goats.</p><p><strong>Methods: </strong>Twelve adult goats were randomly assigned to two groups: a control group (CON) and a treatment group (TAN) that received 2 % tannic acid in their diet. The experiment had two stages, each with a 10 - day adaptation and a 5 - day sampling phase. Methane emission was measured using a mobile open-circuit respirometry system, while rumen fluid samples were analyzed for volatile fatty acids (VFAs), ammonia nitrogen, and microbial composition via 16S rRNA sequencing.</p><p><strong>Results: </strong>The results indicated that the tannic acid significantly reduced overall methane emission (p < 0.05), methane per voluntary feed intake (VFI, p < 0.05), dry matter intake (DMI, p < 0.05), acid detergent fiber intake (ADFI, p < 0.05), and neutral detergent fiber intake (NDFI, p < 0.05). Microbial analysis showed increased relative abundance of Firmicutes (p < 0.05) and decreased Methanobrevibacter and Prevotella. Tannic acid also improved ADF digestibility (p < 0.05) and altered VFA profiles by increasing acetic acid to propionic acid ratios and valeric and butyric acids (p < 0.05). Tannic acid supplementation also increases the ratios of acetic acid to propionic acid, as well as valeric and butyric acids (p < 0.05), while increasing the digestibility of ADF (p < 0.05).</p><p><strong>Conclusion: </strong>Dietary tannic acid effectively reduced methane emission and enhanced feed efficiency in Liuyang black goats by modifying rumen fermentation and microbial activity. These findings suggest tannic acid's potential as a sustainable feed additive for ruminants, although long-term impacts on health and productivity require further investigation.</p>\",\"PeriodicalId\":7825,\"journal\":{\"name\":\"Animal Bioscience\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal Bioscience\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.5713/ab.25.0114\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Bioscience","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.5713/ab.25.0114","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
摘要
目的:研究饲粮中添加单宁酸对浏阳黑山羊甲烷产量、营养物质消化率、瘤胃发酵及瘤胃微生物群的影响。方法:将12只成年山羊随机分为对照组(CON)和治疗组(TAN),治疗组在日粮中添加2%的单宁酸。试验分为两个阶段,每个阶段为10天的适应期和5天的采样期。使用移动开路呼吸测量系统测量甲烷排放,同时通过16S rRNA测序分析瘤胃液样品的挥发性脂肪酸(VFAs)、氨氮和微生物组成。结果:结果表明,单宁酸显著降低了总甲烷排放量(p < 0.05)、每自愿采食量甲烷量(VFI, p < 0.05)、干物质采食量(DMI, p < 0.05)、酸性洗涤纤维采食量(ADFI, p < 0.05)和中性洗涤纤维采食量(NDFI, p < 0.05)。微生物学分析显示厚壁菌门相对丰度增加(p < 0.05),甲烷杆菌和普雷沃氏菌相对丰度降低。单宁酸还能提高ADF的消化率(p < 0.05),并通过提高乙酸/丙酸比率、戊酸和丁酸来改变VFA分布(p < 0.05)。添加单宁酸还提高了乙酸/丙酸、戊酸和丁酸的比值(p < 0.05),提高了ADF的消化率(p < 0.05)。结论:饲粮单宁酸通过改变瘤胃发酵和微生物活性,有效减少浏阳黑山羊甲烷排放,提高饲料效率。这些发现表明单宁酸作为反刍动物可持续饲料添加剂的潜力,尽管对健康和生产力的长期影响需要进一步研究。
Effects of dietary supplementing tannic acid on methane production, rumen fermentation, rumen microflora and nutrient digestion of Liuyang black goats.
Objective: This study investigated the effects of dietary tannic acid on methane production, nutrient digestibility, rumen fermentation, and rumen microbiota in Liuyang black goats.
Methods: Twelve adult goats were randomly assigned to two groups: a control group (CON) and a treatment group (TAN) that received 2 % tannic acid in their diet. The experiment had two stages, each with a 10 - day adaptation and a 5 - day sampling phase. Methane emission was measured using a mobile open-circuit respirometry system, while rumen fluid samples were analyzed for volatile fatty acids (VFAs), ammonia nitrogen, and microbial composition via 16S rRNA sequencing.
Results: The results indicated that the tannic acid significantly reduced overall methane emission (p < 0.05), methane per voluntary feed intake (VFI, p < 0.05), dry matter intake (DMI, p < 0.05), acid detergent fiber intake (ADFI, p < 0.05), and neutral detergent fiber intake (NDFI, p < 0.05). Microbial analysis showed increased relative abundance of Firmicutes (p < 0.05) and decreased Methanobrevibacter and Prevotella. Tannic acid also improved ADF digestibility (p < 0.05) and altered VFA profiles by increasing acetic acid to propionic acid ratios and valeric and butyric acids (p < 0.05). Tannic acid supplementation also increases the ratios of acetic acid to propionic acid, as well as valeric and butyric acids (p < 0.05), while increasing the digestibility of ADF (p < 0.05).
Conclusion: Dietary tannic acid effectively reduced methane emission and enhanced feed efficiency in Liuyang black goats by modifying rumen fermentation and microbial activity. These findings suggest tannic acid's potential as a sustainable feed additive for ruminants, although long-term impacts on health and productivity require further investigation.