Protection of bovine mammary epithelial cells against lipopolysaccharide-induced inflammatory responses using Centella asiatica through its antioxidant and anti-inflammatory activities.

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Do Hyun Kim, Hyuk Cheol Kwon, Jong Hyeon Han, Hyun Su Jung, Dong-Min Shin, Sung Gu Han
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引用次数: 0

Abstract

Background: : Bovine mastitis, an inflammatory condition affecting dairy cow udders, results in decreased milk quantity and quality, posing significant economic losses in the dairy industry. With increasing interest in natural products, Centella asiatica has garnered attention for its potent anti-inflammatory and antioxidant properties. Objective: This study aimed to investigate the potential protective effects of C. asiatica extracts (CE) against lipopolysaccharide (LPS)-induced inflammation in bovine mammary epithelial cells (MAC-T).

Methods: : CEs were prepared by extracting C. asiatica leaf powder using ethanol at concentrations of 60%, 70%, 80%, 90%, and 100%. In LPS-stimulated MAC-T cells, the study investigated the ability of CE to reduce oxidative stress and inflammation and its impact on casein protein synthesis.

Results: : C. asiatica extract with 60% ethanol (CE60) exhibited the highest radical scavenging activity and total phenolic content among the tested extracts. In MAC-T cells, CE60 significantly attenuated LPS-induced inflammation by down-regulating pro-inflammatory cytokines such as cyclooxygenase-2, tumor necrosis factor-α, interleukin (IL)-1β, and IL-6, as well as inhibiting nuclear factor kappa B activation. CE60 also reduced intracellular reactive oxygen species through upregulating nuclear factor erythroid-2-related factor 2 and associated antioxidant enzymes, including glutathione peroxidase (GPx) 1, GPx4, superoxide dismutase (SOD) 1, SOD2, and catalase. Moreover, CE60 restored the synthesis of casein proteins (CSN1S1, CSN1S2, and CSN2) in LPS-treated MAC-T cells, indicating a protective effect on lactation function under inflammatory conditions.

Conclusion: : Taken together, CE60 has potential as a natural substance for the prevention of bovine mastitis by reducing oxidative stress and inflammation in mammary epithelial cells.

积雪草抗氧化和抗炎作用对脂多糖诱导的牛乳腺上皮细胞的保护作用。
背景:牛乳腺炎是一种影响奶牛乳房的炎症,会导致牛奶数量和质量下降,给乳制品行业造成重大经济损失。随着人们对天然产品的兴趣日益浓厚,积雪草因其有效的抗炎和抗氧化特性而受到关注。目的:探讨积山草提取物(CE)对脂多糖(LPS)诱导的牛乳腺上皮细胞(MAC-T)炎症的保护作用。方法:用浓度分别为60%、70%、80%、90%、100%的乙醇提取积雪草叶粉制备ce。在lps刺激的MAC-T细胞中,本研究考察了CE降低氧化应激和炎症的能力及其对酪蛋白合成的影响。结果:60%乙醇浓度(CE60)的积雪草提取物对自由基的清除能力和总酚含量最高。在MAC-T细胞中,CE60通过下调环氧化酶-2、肿瘤坏死因子-α、白细胞介素(IL)-1β和IL-6等促炎细胞因子以及抑制核因子κ B的活化,显著减轻lps诱导的炎症。CE60还通过上调核因子红细胞2相关因子2和相关抗氧化酶,包括谷胱甘肽过氧化物酶(GPx) 1、GPx4、超氧化物歧化酶(SOD) 1、SOD2和过氧化氢酶,减少细胞内活性氧。此外,CE60恢复了lps处理的MAC-T细胞中酪蛋白(CSN1S1、CSN1S2和CSN2)的合成,表明在炎症条件下对泌乳功能有保护作用。综上所述,CE60有可能作为一种天然物质,通过减少乳腺上皮细胞的氧化应激和炎症来预防牛乳腺炎。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
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