The mechanism of m6A methylation analysis of the transcriptome to regulate the diameter of Alpine Merino wool fiber.

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Lin Yue, Ting Ting Guo, Bo Wen Chen, Jian Bin Liu, Zeng Kui Lu, Chao Yuan
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Abstract

Objective: Wool is an important textile raw material, and fiber diameter is a major determinant of the economic value and quality of wool products. Analyses of the regulatory mechanisms underlying wool fiber diameter are necessary for the development of strategies to improve wool fineness. Therefore, we used methylationomics to analyze the skin tissue of individuals with different fiber diameters, and analyzed the apparent regulation mechanism of wool fiber diameter.

Methods: In this study, we jointly analyzed the transcriptome and m6A methylome of skin tissues from individual Alpine Merino sheep with different wool fiber diameters (classified into three groups) to mine key methylated RNAs and explore the significance of m6A methylation in the regulation of this trait.

Result: In total, 54,057 methylated peaks, 4,273 differentially methylated genes, 139 differentially methylated lncRNAs, and 2,992 differentially methylated circRNAs were found in the three comparisons. These loci were enriched in the Wnt, Notch, and TGF-β signaling pathways, as determined through GO and KEGG pathway analyses. RNA correlation analyses revealed key RNAs, such as CACNA1E, FOS, CAMK2B, RNF43, circ-0317, circ-4794, TCONS-00020832, and TCONS-00020845, indicating that hypermethylation may be an important factor affecting wool fiber diameter.

Conclusion: These findings provide insight into the molecular regulatory mechanism underlying wool fiber diameter and provide a theoretical basis for the development of the wool industry.

m6A甲基化转录组调控高山美利奴羊毛纤维直径的机制分析。
目的:羊毛是重要的纺织原料,纤维直径是决定羊毛产品经济价值和质量的主要因素。分析羊毛纤维直径的调节机制对于制定提高羊毛细度的策略是必要的。因此,我们利用甲基化组学对不同纤维直径个体的皮肤组织进行分析,分析羊毛纤维直径的明显调控机制。方法:在本研究中,我们联合分析阿尔卑斯美利奴羊不同羊毛纤维直径个体(分为三组)皮肤组织的转录组和m6A甲基组,挖掘关键甲基化rna,并探讨m6A甲基化在该性状的调控中的意义。结果:三种比较共发现54057个甲基化峰、4273个差异甲基化基因、139个差异甲基化lncrna和2992个差异甲基化环状rna。通过GO和KEGG通路分析发现,这些基因座在Wnt、Notch和TGF-β信号通路中富集。RNA相关分析揭示了CACNA1E、FOS、CAMK2B、RNF43、circ-0317、circ-4794、TCONS-00020832、TCONS-00020845等关键RNA,表明高甲基化可能是影响羊毛纤维直径的重要因素。结论:研究结果揭示了羊毛纤维直径的分子调控机制,为羊毛产业的发展提供了理论依据。
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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
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