Association between Weaning Stress and Rumen Microbiota in Goat Kids: Evidence from Granger Causality and Randomized Controlled Trial Validation.

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Ziwei Peng, Hanjie Xiao, Weiwei Yang, Tong Wang, Haojiong Xie, Hui Yan, Shoukun Ji
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引用次数: 0

Abstract

Objective: Weaning stress is frequently associated with disrupted gastrointestinal microbiota and decreased growth performance, the association between them remains unclear.

Methods: In this study, we employed Granger causality inference to compare the dynamic changes in gastrointestinal microbiota between stressed and non-stressed goat kids, followed by validation through a randomized controlled trial involving rumen microbiota transplantation.

Results: Our findings indicated that the alpha diversity and microbial maturity of the rumen microbiota in stressed kids were significantly lower than those in non-stressed kids during the weaning period (p<0.05). Furthermore, the rumen microbiota at pre-weaning could accurately predict the body weight changes during weaning stress period (R2=0.99, RMSPE=0.19). We demonstrated that the cultured rumen microbiota retained most high-abundance rumen bacteria (89.22%), and its transplantation effectively altered the rumen microbiota (ANOSIM, p<0.05), enhanced microbial maturity (p<0.05), and improved growth performance (p<0.05) during weaning, without affecting the gut microbiota (p>0.05). Additionally, transplantation of the cultured rumen microbiota reduced intestinal permeability and inflammation while increasing antioxidant levels in weaned kids(p<0.05).

Conclusion: These findings provide evidence for the association between rumen microbiota and weaning stress, demonstrating that manipulating rumen microbiota is an effective strategy for alleviating weaning stress in goat kids.

断奶应激与山羊瘤胃微生物群的关系:来自格兰杰因果关系和随机对照试验验证的证据。
目的:断奶应激通常与胃肠道微生物群紊乱和生长性能下降有关,两者之间的关系尚不清楚。方法:在本研究中,我们采用格兰杰因果推理来比较应激和非应激山羊羔羊胃肠道微生物群的动态变化,然后通过随机对照试验进行瘤胃微生物群移植验证。结果:断奶期应激组瘤胃微生物群α多样性和微生物成熟度显著低于非应激组(p0.05)。此外,移植培养的瘤胃微生物群降低了断奶山羊的肠道通透性和炎症,同时增加了抗氧化水平。结论:这些发现为瘤胃微生物群与断奶应激之间的关联提供了证据,表明调节瘤胃微生物群是缓解断奶应激的有效策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
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