Longitudinal dynamics of plasma bile acids and their associations with physiological parameters and fecal microbiome during the transition period in dairy cows.

IF 2.4 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Bioscience Pub Date : 2025-06-01 Epub Date: 2025-02-27 DOI:10.5713/ab.24.0628
Feixiang Fan, Liang Chen, Huizeng Sun, Jianxin Liu, Kailun Yang, Fengfei Gu
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引用次数: 0

Abstract

Objective: The aim of this study is to investigate the dynamic changes of plasma bile acids (BA) and their correlations with physiological parameters and fecal microbiome in transitional dairy cows.

Methods: Twenty multiparous dairy cows were selected, the blood and fecal samples were collected on d -21, -7, +7, and +21 of calving. The targeted metabolome and 16s rDNA ampicon sequencing were utilized to identify BA profiles and fecal microbial composition, respectively.

Results: A total of 32 BAs were found, comprising 9 primary BAs (PBA) and 23 secondary BAs (SBA). Majority of the PBAs (7 out to 9) and SBAs (15 out to 23) exhibited significant increases postpartum compared to prepartum levels. Notably, ursodeoxycholic acid, taurocholic acid and 7-ketodeoxycholic acid showed higher importance. Correlation analysis showed the BAs concentrations positively correlated with the concentrations of aspartate aminotransferase, total antioxidant capacity, and glutathione peroxidase, while exhibiting substantial negative correlation with triglyceride concentrations. A decline in bacterial alpha diversity in postpartum and significantly different β-diversity were observed. Furthermore, 30 distingtive genera were identified over the transition period. Among these, six and eleven biomarkers such as Alistipes and Ruminococcaceae_UCG_014 were identified at +7 d and +21 d, respectively. Furthermore, the abundances of choloylglycine hydrolase and 7-alpha-hydroxysteroid dehydrogenase which are involved in SBA biosynthesis were significantly higher postpartum as determined by PICRUSt2 analysis over the transition period.

Conclusion: The BA profile and concentrations underwent significant changes during the transition period in dairy cows and these changes are closely related to the periparturient health of the cows. Ursodeoxycholic acid and Alistipes was identified as the pivotal BA and microbial genus. Our study elucidates these metabolic processes, providing useful insights into strategies for enhancing the nutrition and well-being of perinatal dairy cows.

奶牛过渡期血浆胆汁酸的纵向动态及其与生理参数和粪便微生物群的关系
目的:研究过渡期奶牛血浆胆汁酸(BA)的动态变化及其与生理代谢和粪便微生物群的关系。方法:选取20头产犊奶牛,分别于产犊d -21、-7、+7和+21采集血液和粪便样本。应用靶向代谢组和16s rDNA基因测序分别检测BA谱和粪便微生物组成。结果:共鉴定出32种BA,其中一级BA (PBA) 9种,二级BA (SBA) 23种。大多数PBAs(7 / 9)和SBAs(15 / 23)产后与产前相比显著增加。其中,熊去氧胆酸、牛磺胆酸和7-酮去氧胆酸的重要性较高。相关分析显示,BAs浓度与天冬氨酸转氨酶、总抗氧化能力和谷胱甘肽过氧化物酶浓度呈正相关,与甘油三酯浓度呈显著负相关。产后细菌α多样性下降,β多样性差异显著。此外,在过渡时期鉴定出30个显著不同的属。其中,在+7d和+21d分别鉴定出6个和11个生物标志物,如Alistipes和Ruminococcaceae_UCG_014。此外,PICRUSt2分析表明,参与SBA生物合成的胆酰甘氨酸水解酶和7- α -羟基类固醇脱氢酶的丰度在产后显著高于过渡期。结论:这些发现强调了产后奶牛对ba的需求大幅增加,并强调了ba对牛健康的潜在影响。通过揭示这些代谢动力学,我们的研究为优化围产期奶牛的营养和健康提供了有价值的见解。
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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
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