Aquaculture最新文献

{"title":"Corrigendum to “Effects of different LED lights colors on the feeding behavior of Chinese longsnout catfish (Leiocassis longirostris)” [Volume 612, Part 1, 15 January 2026, 743124]","authors":"Huiying Ma , Meijie Tan , Weiwei Li , Qiyou Xu , Ming Duan","doi":"10.1016/j.aquaculture.2025.743241","DOIUrl":"10.1016/j.aquaculture.2025.743241","url":null,"abstract":"","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743241"},"PeriodicalIF":3.9,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined amino acid mutations in position 67 and 438 in the glycoprotein of infectious hematopoietic necrosis virus can further reduce the pathogenicity","authors":"Shuaibo Li,&nbsp;Rui Ao,&nbsp;Yulu Ma,&nbsp;Xinjia Lv,&nbsp;Min Liu","doi":"10.1016/j.aquaculture.2025.743255","DOIUrl":"10.1016/j.aquaculture.2025.743255","url":null,"abstract":"<div><div>The outbreak of infectious hematopoietic necrosis (IHN), driven by the severe acute IHN virus (IHNV), poses a significant risk to rainbow trout farming in China. As such, the development of effective vaccines and an understanding of their mechanisms are essential. Our previous research demonstrated that mutations at sites 67 and 438 respectively in the G protein of IHNV could reduce its pathogenicity and enhance the survival rate (SR) of rainbow trout post-infection. In this study, we mutated at both amino acid positions 67 and 438 simultaneously in the G protein of IHNV. The resulting recombinant IHNVs (rIHNVs) exhibited similar growth patterns to the parental strain. Additionally, rIHNV-G₆₇_–₄₃₈ induced reduced apoptosis and lower activity of caspase-3 and caspase-9 compared to other groups in vitro. Rainbow trout vaccinated with rIHNV-G₆₇_–₄₃₈ showed significant protection against IHNV, with a relative percent survival (RPS) of approximately 88.9 %, outperforming other groups. Furthermore, vaccination with rIHNV-G₆₇_–₄₃₈ triggered a notable increase in specific IgM antibodies. Notably, an antiviral immune response was rapidly activated early in the vaccination process, evidenced by the upregulation of <em>IL1β</em>, <em>IFN1</em>, <em>IL8</em>, <em>TNF-α</em>, <em>MX1</em>, and <em>TLR3</em>. The viral genomic load in fish vaccinated with rIHNV-G₆₇_–₄₃₈ was significantly lower compared to the other groups. These findings indicate that this vaccine enhances immune responses while reducing viral virulence, making it a promising candidate for the prevention of IHN outbreaks. Additionally, the study provides new insights into the virulence mechanisms of rhabdoviruses and the development of attenuated vaccines.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743255"},"PeriodicalIF":3.9,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Health, microbiota, and water quality analysis: A case study in a commercial recirculating aquaculture system for yellowtail kingfish (Seriola lalandi)” Aquaculture, Volume 610, January 2026, 742851","authors":"Katerina Loufi ,&nbsp;Julia Hassa ,&nbsp;Eric Hernández ,&nbsp;Marit Stormoen ,&nbsp;Deni Deni Ribičić ,&nbsp;Stamatios Kapasakis ,&nbsp;Tobias Busche ,&nbsp;Jörn Kalinowski ,&nbsp;Pavlos Makridis ,&nbsp;Roman Netzer ,&nbsp;Elia Ciani","doi":"10.1016/j.aquaculture.2025.743240","DOIUrl":"10.1016/j.aquaculture.2025.743240","url":null,"abstract":"","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743240"},"PeriodicalIF":3.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural lipid profile mimicking diets to enhance survival and fitness of restocked salmon parr in river ecosystems?","authors":"Valérie Cornet,&nbsp;Anna Wanderscheid,&nbsp;Camille Carpentier,&nbsp;Alexandre Erraud,&nbsp;Yvan Larondelle,&nbsp;Christel Lefrançois,&nbsp;Patrick Kestemont","doi":"10.1016/j.aquaculture.2025.743259","DOIUrl":"10.1016/j.aquaculture.2025.743259","url":null,"abstract":"<div><div>Atlantic salmon parr (<em>Salmo salar</em>) reared in hatcheries for restocking in rivers are typically fed high-lipid commercial diets that diverge from natural prey profiles. This may have a detrimental effect on their ecological adaptation. This study evaluated whether diets mimicking natural lipid levels (12.7 % vs. 20 %) and fatty acid compositions (plant-derived α-linolenic acid ALA vs. marine eicosapentaenoic acid EPA), or with partial chironomid supplementation, enhance fitness traits essential for wild survival. Six dietary treatments were evaluated over a 47-day period, with parameters including growth, lipid metabolism, swimming performance, and immune responses pre- and post-bacterial challenge with <em>Aeromonas salmonicida salmonicida</em>. The results demonstrated that reducing dietary lipids to 12.7 % led to a significant decrease in fat accumulation while maintaining optimal growth rates. EPA12.7 % diets elevated citrate synthase activity, indicating enhanced aerobic capacity, whereas ALA20% diets increased lactate dehydrogenase activity, suggesting earlier anaerobic reliance during sustained swimming. Innate immune gene (<em>c3, lysozyme, igm, mhc, cox2, mpo</em>) expression remained unaffected post-infection across diets, with no mortality observed. Chironomid supplementation resulted in a reduction in lipid content compared to the commercial diet but also led to impaired feed conversion efficiency due to feed intake reduction. The findings demonstrate that 12.7 % lipid diets with EPA enrichment optimize metabolic capacity and reduce adiposity, aligning salmon parr physiology closer to wild counterparts. While plant-based oils did not compromise immunity, the inclusion of chironomid necessitated dosage adjustments to balance predation training and energy intake. This dietary strategy could enhance restocking success by mitigating the effects of feeding-related domestication.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743259"},"PeriodicalIF":3.9,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic prediction of scale drop disease resistance in barramundi Lates calcarifer under controlled laboratory conditions","authors":"Zhi Weng Josiah Poon ,&nbsp;Nguyen Thanh Vu ,&nbsp;Xueyan Shen ,&nbsp;Susan Gibson-Kueh ,&nbsp;Maura Carrai ,&nbsp;Sarah Priyanka Nelson ,&nbsp;Celestine Terence ,&nbsp;Ying Quan Tan ,&nbsp;Sunita Awate ,&nbsp;Ha Thanh Dong ,&nbsp;Saengchan Senapin ,&nbsp;Marie R. Tan ,&nbsp;Shubha Vij ,&nbsp;David B. Jones ,&nbsp;Dean R. Jerry ,&nbsp;Jose A. Domingos","doi":"10.1016/j.aquaculture.2025.743250","DOIUrl":"10.1016/j.aquaculture.2025.743250","url":null,"abstract":"<div><div>Barramundi (<em>Lates calcarifer</em>) is a tropical aquaculture species principally farmed across Southeast Asia and Australia. However, outbreaks of scale drop disease virus (SDDV) have caused substantial economic losses due to mass mortalities of 40 % to 90 % on farms in Singapore. While vaccines against SDDV are under development, selective breeding for resistance offers a promising avenue for long term disease management. Although moderate heritability against SDDV has been observed in farm environments, the development of controlled laboratory studies, free of interference from external factors and co-infections, is necessary to provide a clearer understanding of genetic resistance and accelerate the development of SDDV-resistant barramundi strains. This study established a laboratory infection model for juvenile barramundi to determine the appropriate SDDV dose via intraperitoneal injections required to induce approximately 50 % mortality over 21 days. Subsequently, two challenge trials involving 654 (80.1 ± 16.1 g) and 705 (68.6 ± 12.0 g) juveniles were conducted under controlled conditions in a single 6000 L recirculating aquaculture system, with survival rates of 5.8 % and 34.2 % respectively. Three SDDV resistant traits were assessed: survival time (days survived), survival status (alive or dead at day 21), and survival 50 % (alive or dead on the day closest to 50 % survival). Genomic prediction models were developed using GBLUP and bayesR approaches based on ∼48 k SNPs from a 70 K barramundi SNP Array. Heritability estimates ranged from 0.16 to 0.42 for survival time, 0.06 to 0.33 for survival status, and 0.26 to 0.35 for survival 50 %. Prediction accuracies were high across traits, from 0.75 to 0.91 for survival time, 0.51 to 0.63 for survival status, and 0.72 to 0.90 for survival 50 %, confirming the feasibility of genomic selection for developing SDDV-resistant barramundi lines. These findings provide a strong foundation for integrating disease resistance into selective breeding programmes, offering a strategy to mitigate SDDV-related losses and strengthening the resilience of barramundi aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743250"},"PeriodicalIF":3.9,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From recognition to defense: the multifaceted functions of PoPGRP-2 and PoPGRP-5 in Paralichthys olivaceus immunity","authors":"Yue Fang ,&nbsp;Xiao-Dong Xu ,&nbsp;Sheng-Han Gao ,&nbsp;Jian Zhang ,&nbsp;Xue-Peng Li","doi":"10.1016/j.aquaculture.2025.743252","DOIUrl":"10.1016/j.aquaculture.2025.743252","url":null,"abstract":"<div><div>Peptidoglycan recognition proteins (PGRPs) are an indispensable constituent in innate immunity, capable of identifying microbial peptidoglycan (PGN) and exert crucial effects in antibacterial defense. In this paper, we explored the immunological characteristics of two types of PGRP in <em>Paralichthys olivaceus</em>, i.e., the long PGRP, PoPGRP-2 and the short PGRP, PoPGRP-5. Both PoPGRP-2 (484 amino acid) and PoPGRP-5 (167 aa) possess a conserved PGRP domain and four conserved Zn²⁺-binding residues which determines their amidase capacity. The <em>PoPGRP-2</em> and <em>PoPGRP-5</em> were constitutive present in various tissues, and markedly regulated by bacterial infection. rPoPGRP-2 and rPoPGRP-5 were capable of binding to and clumping bacteria, and exhibited inhibitory and bactericidal properties in a Zn²⁺-dependent manner. Furthermore, in vitro both rPoPGRP-2 and rPoPGRP-5 could induce peripheral blood leukocytes' (PBLs)’ proliferation, phagocytosis, and ROS production via their attachment to the membrane of PBLs. Finally, in vivo both rPoPGRP-2 and rPoPGRP-5 could suppressed the microbial infection in immune related tissues. In conclusion, our observations deliver the initial proof that both PoPGRP-2 and PoPGRP-5 are vital in antimicrobial immunity of flounder.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743252"},"PeriodicalIF":3.9,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivation of the polychaete worm Sabella spallanzanii (Gmelin, 1791) in a novel multi-species IMTA (integrated multi-trophic aquaculture) system in the Mediterranean Sea","authors":"Daniele Arduini ,&nbsp;Sergio Rossi ,&nbsp;Danilo Migoni ,&nbsp;Adriana Giangrande","doi":"10.1016/j.aquaculture.2025.743248","DOIUrl":"10.1016/j.aquaculture.2025.743248","url":null,"abstract":"<div><div>The cultivation and biomass production of the worm <em>Sabella spallanzanii</em> (Gmelin, 1791) through local macrofouling recruitment on natural fiber ropes was investigated in an IMTA-converted fish farm in the Mediterranean Sea. Over a 19-month cultivation cycle, a species-rich macrofouling assemblage was obtained, reaching a final biomass of 19–24 kg per rope, with only four species (<em>S. spallanzanii</em>, <em>Mytilus galloprovincialis</em> Lamarck, 1819, <em>Phallusia mammillata</em> (Cuvier, 1815), and <em>Paraleucilla magna</em> Klautau, Monteiro &amp; Borojevic, 2004) contributing to 86 ± 7 % of total biomass. Each species likely found favorable settlement conditions, exploiting available food resources and potentially helping to reduce/recycle excess organic matter. Despite high mortality, <em>S. spallanzanii</em> accounted for 66 ± 7 % of final macrofouling biomass, with about 210,000 worms (≈1-ton live biomass) produced at IMTA farm scale using 196 10-m-long ropes. The observed decline in abundance during the cultivation cycle (from 3393 to 1090 individuals per rope) followed an exponential decay model, while individual growth (0.25–5.06 g) was described by a logistic curve, projecting the optimal harvest time between months 22–30, with a biomass peak of 1.57 ± 0.85 tons. Chemical analyses showed elevated heavy metal concentrations in worm tubes and crowns, but body tissues remained below EU regulatory thresholds, supporting their safe use in fish feed. Additionally, due to their antimicrobial mucus and aesthetic features, worms show potential for both pharmaceutical and ornamental aquarium markets. These findings support <em>S. spallanzanii</em> as a valuable species for cultivation within IMTA systems, further highlighting the potential of macrofouling exploitation for integrated biomass valorization.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743248"},"PeriodicalIF":3.9,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary ferulic acid improved growth performance, health status, digestive capacity, and glycolipid metabolism of juvenile Chinese Mitten Crab (Eriocheir sinensis) fed with low fish meal diets","authors":"Jiaqi He,&nbsp;Xuming Lu,&nbsp;Yuanyi Xin,&nbsp;Jinquan Fan,&nbsp;Wei Liu,&nbsp;Song Wang,&nbsp;Liqiao Chen,&nbsp;Erchao Li,&nbsp;Xiaodan Wang","doi":"10.1016/j.aquaculture.2025.743246","DOIUrl":"10.1016/j.aquaculture.2025.743246","url":null,"abstract":"<div><div>High plant protein diets can induce oxidative stress and tissue damage, disrupt glucose and lipid metabolism, and ultimately impair the growth of aquatic animals. This study examined the effects of ferulic acid on growth performance, health status, digestive function and glycolipid metabolism in juvenile <em>Eriocheir sinensis</em> fed a high plant protein diet. A total of 960 juvenile crabs (mean weight: 0.50 ± 0.02 g) were randomly allocated to six experimental groups and fed either a control diet (35 % fishmeal) or low fishmeal diets (15 % fishmeal, with concentrations of 0, 40, 80, 160, and 320 mg/kg ferulic acid) for 56 days. Compared with juvenile crabs fed high-fishmeal diets, those fed low-fishmeal diets exhibited reduced weight gain rates and impaired antioxidant capacity, digestive function, and glucose-lipid metabolism. But compared to the low-fishmeal control group, the inclusion of 80–160 mg/kg ferulic acid showed the higher weight gain rate and higher specific growth rate of juvenile crabs, and lowered the feed conversion ratio. Ferulic acid supplementation at levels of 40–160 mg/kg enhanced total antioxidant capacity (T-AOC) in the hepatopancreas, reduced aspartate aminotransferase (AST) levels in hemolymph, and decreased malondialdehyde (MDA) levels in the hepatopancreas. Additionally, a 160 mg/kg ferulic acid supplementation improved the histology of intestinal tissues. Ferulic acid also promoted lipid biosynthesis, causing a substantial rise in the crude lipid content of the whole crab, as well as elevated hemolymph triglyceride (TG), cholesterol (TCHO), and high-density lipoprotein (HDL) levels. Furthermore, ferulic acid upregulated the expression and activity of key genes and enzymes involved in glycolysis, gluconeogenesis, and the pentose phosphate pathway. Conversely, the expression of glycogen synthase (<em>gs</em>) and glycogen phosphorylase (<em>gp</em>) was downregulated. In conclusion, supplementation with ferulic acid in a high plant protein diet significantly enhanced the growth, health, and lipid accumulation in juvenile crabs, while also promoting the utilization of carbohydrates for energy production. According to the regression analysis of weight gain rate and crude lipid content, the optimal supplementation range of ferulic acid was determined to be 123.90–126.09 mg/kg.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743246"},"PeriodicalIF":3.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic regions and candidate genes associated with feed efficiency traits in large yellow croaker (Larimichthys crocea) across different thermal environments","authors":"Pengxin Jiang ,&nbsp;Miaosheng Feng ,&nbsp;Yuxuan Liu ,&nbsp;Junjia Zeng ,&nbsp;Junzhu Xiao ,&nbsp;Qiaozhen Ke ,&nbsp;Longyu Chen ,&nbsp;Jiaying Wang ,&nbsp;Shuimu Hu ,&nbsp;Zongbao Zhang ,&nbsp;Yuxiang Zhang ,&nbsp;Peng Xu","doi":"10.1016/j.aquaculture.2025.743227","DOIUrl":"10.1016/j.aquaculture.2025.743227","url":null,"abstract":"<div><div>Understanding the biological mechanisms underlying feed efficiency (FE) traits is an important issue in addressing environmental challenges and improving farm economics. This study aimed to identify genomic regions and signaling pathways associated with two indicator traits of FE, feed conversion ratio (FCR) and residual feed intake (RFI), in large yellow croaker (<em>Larimichthys crocea</em>). In this study, we evaluated the FE phenotypes of the same group of fish under three different thermal environments (15 °C, 19 °C, and 24 °C). At 15 °C, 6 single nucleotide polymorphisms (SNPs) were associated with FCR_{15 °C}, 3 with RFI_{15 °C}, and 1 with both traits. A total of 31 genes, including <em>slc12a7</em>, <em>slc45a4</em>, <em>apn</em>, <em>dgat1</em>, <em>far1</em>, <em>chka</em>, <em>pappa2</em>, and <em>neurod1</em>, were identified as candidate genes that may affect FE traits through biological processes such as nutrient transport, lipid synthesis and metabolism, and insulin and insulin-like growth factor 1 (IGF-1) signaling pathway. At 19 °C, no SNPs were associated with FCR_{19 °C}, while 7 SNPs with RFI_{19 °C}. A total of 23 genes, including <em>myc</em>, <em>slc25a29</em>, <em>wars</em>, <em>dio3</em>, <em>col2a1</em>, and <em>col5a2</em>, were identified as candidate genes that may affect FE traits through biological processes such as amino acid metabolism, protein synthesis, and cellular proliferation and differentiation. At 24 °C, 7 SNPs were associated with FCR_{24 °C}, 6 with RFI_{24 °C}, and 5 with both traits. A total of 14 genes, including <em>pqlc2</em>, <em>golim4</em>, <em>zbbx</em>, <em>kmt2d</em>, <em>alkbh8</em>, <em>che</em>, <em>aasdhppt</em>, <em>gucy1a2</em>, and <em>serpini1</em>, were identified as candidate genes that may affect FE traits through biological processes such as amino acid and protein transport, DNA repair and transcription regulation, signal transduction and neural function. In addition, all of these candidate genes were enriched in several GO terms, including melanocyte-stimulating hormone receptor activity, transcription cis-regulatory region binding, and microtubule. This study provides valuable insights into the genetic basis of FE traits and identifies SNPs involved in marker-assisted breeding selection of FCR and RFI in large yellow croaker.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743227"},"PeriodicalIF":3.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative genomic and functional analyses identify NFKBIA as a key regulator of salinity tolerance in Corbicula mortoni","authors":"Yu Wang ,&nbsp;Yue Min ,&nbsp;Tianyu Yan ,&nbsp;Jianqi Yang ,&nbsp;Shikai Liu ,&nbsp;Qi Li","doi":"10.1016/j.aquaculture.2025.743221","DOIUrl":"10.1016/j.aquaculture.2025.743221","url":null,"abstract":"<div><div>Salinity tolerance is critical for the aquaculture of bivalves, especially for species inhabiting both freshwater and estuarine environments. Enhanced understanding of genetic mechanisms underlying salinity tolerance will support precision breeding and targeted genetic improvement in aquaculture species. In this study, we conducted a comprehensive comparative genomic analysis of <em>Corbicula mortoni</em>, a euryhaline representative of the <em>Corbicula</em> species complex. Our results revealed that immune- and stress-related pathways, particularly the NF-κB signaling pathway, have undergone gene family expansion. Population genomic analysis based on whole-genome resequencing showed that freshwater and estuarine populations formed two distinct clusters, with highly differentiated genomic regions harboring the <em>NFKBIA</em> gene, which is potentially involved in salinity tolerance. Functional validation using RNA interference (RNAi) targeting <em>NFKBIA</em> in the estuarine population under different salinity conditions (5 and 25 psu) showed that knockdown of <em>NFKBIA</em> significantly affected the expression of salinity-related genes and induced marked histological changes in gill tissues. These findings highlight the pivotal role of the NF-κB pathway in the salinity stress of <em>C. mortoni</em> and provide valuable molecular targets for the genetic improvement of salinity tolerance in bivalve aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743221"},"PeriodicalIF":3.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}