Aquaculture最新文献

{"title":"Effects of pathogenic yeast Metschnikowia bicuspidata infection on immune function and intestinal microflora of the Chinese mitten crab Eriocheir sinensis","authors":"Shuang Hao ,&nbsp;Zhang Luo ,&nbsp;Xiaohui Bai ,&nbsp;Hongzheng You ,&nbsp;Xiaolian Liu ,&nbsp;Houfu Liu ,&nbsp;Hongbo Jiang ,&nbsp;Xiaoli Xu","doi":"10.1016/j.aquaculture.2025.742706","DOIUrl":"10.1016/j.aquaculture.2025.742706","url":null,"abstract":"<div><div>Chinese mitten crab (<em>Eriocheir sinensis</em>) aquaculture has been significantly impeded by “milky disease,” which is caused by the pathogenic yeast <em>Metschnikowia bicuspidata.</em> To investigate the effects of infection by this parasite on the Chinese mitten crab, a suspension of <em>M. bicuspidata</em> (1 × 10⁷ CFU/mL) was injected into healthy crabs at the membrane of the fourth dactyl joint. The changes of non-specific immune-related enzyme activities and the expression levels of related genes in the hemolymph and hepatopancreas of crabs were monitored after infection, as were the effects on the structure of intestinal microflora. In the serum and hepatopancreas, activities of superoxide dismutase and catalase gradually decreased; those of glutathione peroxidase, alkaline phosphatase, and acid phosphatase gradually increased; that of lysozyme increased first and then decreased; and total antioxidant capacity fluctuated over time. Post-infection, the expression levels of several Toll signaling pathway-related genes, including <em>Toll1</em>, <em>Toll2</em>, <em>MyD88</em>, and <em>Dorsal</em>, in the hemocytes and hepatopancreas of crabs were mainly upregulated between 6 and 96 h, with significant downregulation at 192 h. The expression of <em>LZM</em> and <em>Crustin</em> was induced by <em>M. bicuspidata</em> and reached peak levels at 48 or 96 h. The <em>proPO</em> gene was continuously upregulated throughout the experimental period and still exhibited a high expression level at 192 h. The expression trends of <em>Toll1</em>, <em>Toll2,</em> and <em>proPO</em> were similar in hemocytes and the hepatopancreas. The intestinal microbial community composition also changed. At the phylum level, the relative abundance of Firmicutes decreased and the relative abundance of Proteobacteria increased after infection. At the genus level, Gram-negative bacteria such as <em>Aeromonas</em> and <em>Bacteroides</em> were abundant at 96 h post-infection. As infection time increased, the abundance of conditional pathogens such as <em>Shewanella</em>, <em>Vibrio</em>, and <em>Mycobacterium</em> increased. Overall, the results showed that <em>M. bicuspidata</em> induced oxidative stress and inflammatory immune responses in crabs. Infection also caused changes in the intestinal microflora structure, which affected the host's intestinal microecological balance and immune function. These results provided a reference for understanding the pathogenesis of “milky disease” in <em>E. sinensis</em> and devising prevention strategies.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"608 ","pages":"Article 742706"},"PeriodicalIF":3.9,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consistent color preference of largefin longbarbel catfish (Mystus macropterus) across life stages: Implications for aquaculture","authors":"Miao Xiang ,&nbsp;Haoran Liu ,&nbsp;Nian Wei ,&nbsp;Mulan Liao ,&nbsp;Siqi Chen ,&nbsp;Zihao Meng ,&nbsp;Yongjiu Zhu ,&nbsp;Deguo Yang ,&nbsp;Xiaoli Li ,&nbsp;Xuemei Li","doi":"10.1016/j.aquaculture.2025.742705","DOIUrl":"10.1016/j.aquaculture.2025.742705","url":null,"abstract":"<div><div>This study systematically investigated the background color preferences of largefin longbarbel catfish (<em>Mystus macropterus</em>) at different life-history stages and assessed their potential applications in aquaculture practices. The study examined three key developmental stages: 0+, 1+ and 2+ years. It analyzed the selection behavior of <em>M. macropterus</em> in response to six distinct background colors (black, blue, red, white, yellow, and green). The results showed that <em>M. macropterus</em> exhibited a significant preference for black and blue backgrounds at all life-history stages, with this preference remaining consistent across different age groups. This finding challenges the prevailing view that fish color preferences change with life-history stage. Instead, it suggests that the color preferences of <em>M. macropterus</em> are closely related to their stable growth and reproductive strategies, supporting the idea that these preferences constitute a stable “fish personality” across life-history stages. Modulating environmental color is hypothesized to reduce intraspecific aggression in aquaculture environments, thereby providing a direct basis for mitigating aggression and facilitating high-density aquaculture through environmental design. These findings provide novel insights into the behavioral adaptations of <em>M. macropterus</em> and offer practical guidance for optimizing aquaculture environments. This study provides a scientific foundation for future aquaculture management strategies, suggesting that adjusting the background color of aquaculture ponds can simultaneously enhance fish welfare, production efficiency, and economic benefits.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"608 ","pages":"Article 742705"},"PeriodicalIF":3.9,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the effects of low temperature stress on the intestine-hepatopancrea axis and serum metabolites in common carp (Cyprinus carpio)","authors":"Liang Li ,&nbsp;Yi Wang ,&nbsp;Jia Liu ,&nbsp;Zhiyuan Xue ,&nbsp;Peizhong Yu ,&nbsp;Jiawei Dong ,&nbsp;Shengqiang Tao ,&nbsp;Zhiyong Yang ,&nbsp;Yuhong Yang","doi":"10.1016/j.aquaculture.2025.742699","DOIUrl":"10.1016/j.aquaculture.2025.742699","url":null,"abstract":"<div><div>Global climate change has intensified extreme cold events, seriously threatening aquaculture productivity. Although it is recognized that low temperature stress adversely affect the growth and immune function of fish, the specific mechanisms governing the communication between multiple organs, especially the interaction between intestines and hepatopancreas, are still not clear. To reveal the effects of low temperature stress on the intestine-hepatopancrea axis and serum metabolites in common carp (<em>Cyprinus carpio</em>), 120 common carp were divided into 2 groups, including normal temperature group (NT, 25 ± 1 °C) and low temperature group (LT, 5 ± 1 °C). After 48 h low temperature stress, histomorphological, serum biochemical indexes, 16S rDNA sequencing and metabolomics analysis were executed. These results showed that low temperature stress led to intestinal morphological changes (decrease in the number of goblet cells and villus erosion), increased permeability, and triggered multiple stress-related responses including oxidative stress, inflammation, apoptosis, and pyroptosis, led to microbiota dysbiosis with reduced beneficial bacteria and increased pathogenic bacteria. Hepatopancreatic damage included blurred hepatocytes boundaries, nucleus pushed to the periphery, lipid aggregates depletion, elevated the levels of ALT, AST and LDH in serum, the occurrence of oxidative stress, inflammation, apoptosis and pyroptosis. Serum metabolomics analysis showed that ABC transporters, cholesterol metabolism, primary bile acid biosynthesis, nucleotide metabolism and efferocytosis were enriched, with reduced lysophospholipids and increased taurochenodeoxycholic acid. Compensatory responses to low temperature stress included the changes in tight junction protein (<em>ZO-1</em>, <em>Occludin</em>, <em>Claudin-3</em> and <em>Claudin-7</em> mRNA), anti-inflammatory (<em>TGF-β</em> and <em>IL-10</em> mRNA) and anti-apoptotic (<em>Bcl-2</em> mRNA), and probiotics (<em>Caulobacter</em>, <em>Akkermansia</em> and <em>Phenylobacterium</em>). In conclusion, low temperature stress disrupted intestinal barrier integrity and microbiota balance, induced hepatopancreatic structural damage and disorder of cholesterol and bile acid metabolism, ultimately establishing a “intestine leakage-hepatopancreatic injury-metabolic dysregulation” vicious cycle. These findings provide novel insights into the effects of low temperature on the intestine-hepatopancrea axis.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742699"},"PeriodicalIF":3.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"YOLO-feed: An advanced lightweight network enabling real-time, high-precision detection of feed pellets on CPU devices and its applications in quantifying individual fish feed intake","authors":"Miaosheng Feng ,&nbsp;Pengxin Jiang ,&nbsp;Yuhang Wang ,&nbsp;Shuimu Hu ,&nbsp;Sijing Chen ,&nbsp;Rui Li ,&nbsp;Haibin Huang ,&nbsp;Ning Li ,&nbsp;Boyu Zhang ,&nbsp;Qiaozhen Ke ,&nbsp;Yu Zhang ,&nbsp;Peng Xu","doi":"10.1016/j.aquaculture.2025.742700","DOIUrl":"10.1016/j.aquaculture.2025.742700","url":null,"abstract":"<div><div>With the rapid advancement of deep learning, its application in aquaculture has grown significantly. However, the widespread use of deep learning models in this field is hindered by the large number of parameters and high computational power requirements. Moreover, accurately quantifying individual feed intake is essential for genetic improvement of feed efficiency, scientific feeding practices, and increasing aquaculture yield. Unfortunately, existing methods struggle to operate in real-time on low-performance hardware. To address these challenges, this study proposes YOLO-Feed, a cost-effective and highly efficient deep learning network. YOLO-Feed significantly reduces model size by eliminating the large object detection head and its corresponding branch network from You Only Look Once (YOLO) v8s, while introducing a powerful intersection-over-union (IoU) loss to enhance performance. We trained the model on over 9000 labeled feed pellet images. The YOLO-Feed model achieved a precision of 0.966, comparable to YOLOv8s, while reducing the number of parameters to less than 1 million— a 12.3-fold compression. In comparative experiments, YOLO-Feed maintained near-optimal precision with minimal parameters and was the only model capable of processing a single image on a CPU in just 16.9 ms. Furthermore, in an indoor aquaculture experiment involving 1027 fish, YOLO-Feed was successfully applied to measure individual feed intake, achieving an average accuracy of 90.3 % over a 733-min experimental period. These results demonstrate the superior performance and scalability of the YOLO-Feed model. To expedite research on the genetic improvement of feeding efficiency, we have open - sourced this project on GitHub (<span><span>https://github.com/miaomiaoge/YOLO-Feed</span><svg><path></path></svg></span>).</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"608 ","pages":"Article 742700"},"PeriodicalIF":3.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of a new strain of Ictalurid herpesvirus 1 isolated from channel catfish in China","authors":"Fei Yu ,&nbsp;Jiehua Xu ,&nbsp;Sheng Yuan ,&nbsp;Yi Yang ,&nbsp;Siyang Song ,&nbsp;Kai Hao ,&nbsp;Rui Yuan ,&nbsp;Yu Wang ,&nbsp;Xiaohui Chen ,&nbsp;Zhe Zhao","doi":"10.1016/j.aquaculture.2025.742703","DOIUrl":"10.1016/j.aquaculture.2025.742703","url":null,"abstract":"<div><div>Ictalurid herpesvirus poses a significant threat to global catfish aquaculture. In this study, an Ictalurid herpesvirus 1 (IcHV1) strain, isolated from diseased channel catfish in China, was investigated. Initially, PCR assays using IcHV1 gene primers detected viral gene fragments in the tissues of diseased catfish. Meanwhile, transmission electron microscopy (TEM) confirmed the presence of herpesvirus-like virions. This virus, designated IcHV1-JS01, was isolated through incubation with CCO/BB cells, which was verified via PCR and immunoblotting; and infections with this virus triggered cytopathic effects and syncytia formation in CCO/BB cells. Subsequently, a catfish-challenge experiment proved its high virulence, with extremely high viral loads in dead fish. High-throughput sequencing of IcHV1-JS01 revealed a 133,530-bp genome, which shared high similarity with known viral genome, but harboured numerous variations. Phylogenetic analysis classified IcHV1-JS01 as a member of the genus <em>Ictavirus</em>, representing a unique Chinese isolate. Collectively, these findings will facilitate the development of epidemiological investigations and disease control strategies, protecting the catfish farming industry.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742703"},"PeriodicalIF":3.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoxic stress divergence time and metabolism-associated gut microbiome signatures — The strategy is to oxygenate for fat greenling (Hexagrammos otakii)","authors":"Lei Peng ,&nbsp;Yu Zhan ,&nbsp;Dongxu Gao ,&nbsp;Yiting Wu ,&nbsp;Junru Su ,&nbsp;Ling Zhao ,&nbsp;Yuan Liu ,&nbsp;Xinying Zhang ,&nbsp;Rangman Liu ,&nbsp;Wei Wang ,&nbsp;Zhuang Xue","doi":"10.1016/j.aquaculture.2025.742693","DOIUrl":"10.1016/j.aquaculture.2025.742693","url":null,"abstract":"<div><div>Hypoxic stress has always been closely related to the physiology of fishes, but the impacts on the gut microbiota and metabolism have not been fully resolved. In this study, we analyzed the interaction between gut microbiota and host metabolism during hypoxic stress, and reveal the mechanism of growth retardation in fat greenling (<em>Hexagrammos otakii</em>) subjected to hypoxic stress over time. During ongoing hypoxic stress, the oxidative stress and inflammation responses significantly increased over three days compared to the control group, and these changes were restored over fifteen days. Next, we characterized changing microbial communities at different periods of hypoxic stress, and validated the communities for different fish groups subjected to hypoxic stress, based on which we found the temporal dynamics of gut microbiota. Among groups subjected to hypoxic stress, increased inflammatory (<em>IL-1β</em> and <em>TNF-α</em>) response was associated with sphingosine-1-phosphate (metabolites) and <em>Aliivibrio</em> (microbiota) through three days of hypoxia, as compared to the control group and individuals with shorter hypoxic stress (12<em>h</em>). This pattern under extended hypoxic stress (15 days) suggested that arginine and <em>Exiguobacterium</em> play regulating roles during the adaptation process (7–9 days) in <em>H. otakii</em>. These results have important implications for the understanding of the effects of aquaculture and environmental conditions upon <em>H. otakii</em> and offer potential contributions to an ongoing assessment of hypoxic effects upon fishery resources.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742693"},"PeriodicalIF":3.9,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Broodstock genetics, more than ploidy alone, explains oyster resilience at farm sites impacted by sudden unusual mortality syndrome","authors":"Tal Ben-Horin ,&nbsp;Corinne Audemard ,&nbsp;Mark Ciesielski ,&nbsp;Tom Clerkin ,&nbsp;Nicole Krogman ,&nbsp;Jonathan Lucas ,&nbsp;Rachel T. Noble ,&nbsp;Jessica Moss Small ,&nbsp;Kimbery Reece ,&nbsp;Ami Wilbur","doi":"10.1016/j.aquaculture.2025.742691","DOIUrl":"10.1016/j.aquaculture.2025.742691","url":null,"abstract":"<div><div>Oyster (<em>Crassostrea virginica</em>) aquaculture expansion has been met with frequent mortality, particularly in the southeastern United States during warm months from the spring through early fall. This phenomenon has come to be known as Sudden Unusual Mortality Syndrome (SUMS), and observations have implicated triploid oysters as particularly susceptible to SUMS. To better understand the relative contribution of broodstock genetics and ploidy on the susceptibility of oysters to both SUMS-associated pathology and mortality, we produced diploid and triploid oysters from North Carolina and Virginia broodstock and deployed these oysters to seven field sites across North Carolina and Virginia. Dramatic mortality across commercial oyster farms in North Carolina was reported while this field deployment was underway, with farmer-reported mortalities exceeding 90 % of near market-sized oysters. We observed oyster mortality in line with these grower-reported observations, particularly in Virginia diploids deployed to sites in North Carolina coastal sounds. We found the effects of ploidy to depend on the genetic background of deployed oysters, with North Carolina triploid lines experiencing greater mortality than North Carolina diploids, but with Virginia triploids experiencing less mortality than Virginia diploids. Impacted oysters presented with characteristic digestive diverticula pathology that has been consistently associated with SUMS in North Carolina. These results provide unequivocal evidence that pathology and mortality attributed to triploid oysters must be evaluated within the broader context of the genetic background deployed oysters, rather than ploidy alone. Expanded research into the growing areas impacted by SUMS and the commercial oyster stocks widely grown in these areas should be a foremost priority for future research, as is ongoing understanding of variation in SUMS resistance across commercially available oyster lines.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742691"},"PeriodicalIF":3.9,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of spawning season on sperm production and motility in northern pike (Esox lucius): A comparison of stripped and testicular sperm","authors":"Beata Irena Cejko ,&nbsp;Sławomir Krejszeff ,&nbsp;Agata Anna Cejko ,&nbsp;Michał Dubrowski","doi":"10.1016/j.aquaculture.2025.742695","DOIUrl":"10.1016/j.aquaculture.2025.742695","url":null,"abstract":"<div><div>We previously demonstrated that, although there were significant differences in reproductive efficiency (semen volume, sperm concentration, total sperm count) between wild northern pike (<em>Esox lucius</em>) stripped semen (SS) and testicular semen (TS), their motility and kinematic parameters remained similar immediately after collection and after 24 h of short-term storage in an undiluted state during the spawning season (i.e., mid-March). However, longer semen storage of up to 72 h led to decreases in motility and kinematic parameters in SS compared to TS. This indicated that northern pike semen obtained from spermatic ducts lost its biological value (fertilization capacity) much faster than that obtained from testes. Here, for the first time, we examined the effect of the spawning season at the beginning (March 22), in the middle (March 30), and at the end (April 13) on the quantity, quality, and motility of wild northern pike SS and TS. The SS total volume (TVS; ml), volume per kg of body weight (VOS; ml kg⁻¹ b.w.), sperm concentration (SC; ×10⁹ ml⁻¹), and sperm count, i.e., total sperm production (TSP; ×10⁹), and total number of sperm per kg of body weight (TNS; ×10⁹ kg⁻¹b.w.) remained constant throughout the spawning season, suggesting stable production regardless of the stage of the season. In contrast, TS exhibited significant decreases in VOS, SC, TSP, and TNS as spawning progressed. The highest values were recorded at the beginning of the season, while no differences between SS and TS were noted at the end of the season. SS had higher motility values at the beginning of the season, but these parameters deteriorated rapidly as the season progressed. In contrast, TS showed greater stability in sperm motility and velocity parameters throughout the spawning season. This stability may be important for management strategies of northern pike reproduction, both in the wild and in breeding programs.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742695"},"PeriodicalIF":3.9,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combating WSSV based crustacean loss through early pond site detection: A review","authors":"Jinsa Elizebeth Paul ,&nbsp;Saisubramanian Nagarajan ,&nbsp;John Bosco Balaguru Rayappan ,&nbsp;Arockia Jayalatha Kulandaisamy","doi":"10.1016/j.aquaculture.2025.742696","DOIUrl":"10.1016/j.aquaculture.2025.742696","url":null,"abstract":"<div><div>White Spot Syndrome Disease (WSD) is a formidable infectious disease that causes substantial instability and presents a major challenge to achieving sustainable aquaculture, especially in shrimp farming. This review emphasizes the need for early detection of WSSV in shrimp, as no effective therapeutics or vaccines have been developed in the past three decades. Early diagnosis of the disease within a short period is vital in reducing its spread and lowering mortality rates. Insights into viral genomics and the identification of infection mechanisms, supported by in-silico studies, have strengthened disease diagnosis and drug development. Beginning with an overview of traditional diagnostic methods such as histopathology and electron microscopy, this review highlights the importance of sensitivity and specificity in diagnostic tools, particularly for on-site applications. Considering the limitations of DNA amplification and serological methods, <em>viz.</em> lengthy detection times, the need for expertise, and variability in results, of late, several combinatorial approaches have been developed. WSSV biosensors will emerge as promising candidates for portable, user-friendly, rapid, and modifiable diagnostic tools soon. Finally, this review discusses the challenges and limitations of current methods, offering future research directions and recommendations aimed at achieving early-stage WSSV detection by integrating the advantages of various techniques.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742696"},"PeriodicalIF":3.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A DNAzyme biosensor for highly sensitive and rapid detection of decapod iridovirus 1","authors":"Shuai Zhang ,&nbsp;Siying Li ,&nbsp;Huili Tian ,&nbsp;Ming'ao Wang ,&nbsp;Mingsheng Lyu ,&nbsp;Shujun Wang","doi":"10.1016/j.aquaculture.2025.742688","DOIUrl":"10.1016/j.aquaculture.2025.742688","url":null,"abstract":"<div><div>Decapod Iridescent Virus 1 (DIV1) leads to high mortality rates in shrimp farming, significantly impacting the aquaculture industry economically. Currently, there are no effective treatments for controlling DIV1 infections, and management primarily relies on preventative measures. Rapid and precise diagnosis of DIV1 is crucial. In this study, we identified two DNA enzymes, MCP-1 and MCP-8, that can specifically recognize DIV1 via in vitro selection. The detection limit of MCP-1 was 6.89 copies/μL of DIV1. We developed a DNAzyme-based “turn off” biosensor for quick detection of DIV1, which is both highly specific and sensitive, by integrating SYBR Green I to enhance the signal. The detection limit of biosensor could reach 2.01 copies/μL of DIV1. Compared to conventional detection methods, this sensor delivers results in 5 min without the need for complex procedures, greatly streamlining the detection process.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742688"},"PeriodicalIF":3.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}