Aquaculture最新文献

{"title":"Assessing the impact of Lactiplantibacillus plantarum feed additive on Vibrio parahaemolyticus crosstalk and the gill and gut microbiota compositions in Penaeus vannamei","authors":"John Paul Matthew Domingo Guzman ,&nbsp;Samuel Mwakisha Mwamburi ,&nbsp;Kayo Konishi ,&nbsp;Mikio Aoki ,&nbsp;Hiroshi Kuwahara ,&nbsp;Kazuki Mikata ,&nbsp;Keiichiro Koiwai ,&nbsp;Hidehiro Kondo ,&nbsp;Ikuo Hirono","doi":"10.1016/j.aquaculture.2024.742092","DOIUrl":"10.1016/j.aquaculture.2024.742092","url":null,"abstract":"<div><div>Quorum sensing (QS) regulates the expression of genes involved in biofilm formation and production of virulence factors, including the toxin responsible for the acute hepatopancreatic necrosis disease (AHPND). This communication system also influences the structure of bacterial communities. In light of the concept of a “trained microbiome,” which entails host enhanced readiness to respond to subsequent infections, we explored the effects of freeze-dried whole culture of <em>Lactiplantibacillus plantarum</em> (FD-LAB) on the QS system of <em>Vibrio parahaemolyticus</em> AHPND strain as well as on the gill, stomach, and intestinal bacterial communities of <em>P. vannamei</em>. Phenotypic and qPCR analyses showed that FD-LAB inhibits the growth and biofilm formation of <em>V. parahaemolyticus</em> and downregulates key QS genes <em>luxS</em>, <em>opaR</em>, and <em>aphA</em>. Using a high-throughput Illumina sequencing targeting the V4 region of the 16S rRNA gene, we found out that FD-LAB, when used as a dietary supplement, induces specific changes in the gill and gut microbiota. While the changes in alpha and beta diversity indices were mostly statistically nonsignificant, the relative abundances of specific taxa varied upon FD-LAB supplemented feed intake. Specifically, a number of taxa previously reported to be beneficial on shrimp were differentially abundant in the gills. These include members of the families <em>Rhodobacteraceae</em>, <em>Rubritaleaceae</em>, and <em>Pseudoalteromonadaceae</em>, to name a few. Meanwhile, actinomycetes, lactic acid bacteria, and bacilli were some of the beneficial bacterial groups which were enriched in the stomach and intestinal tissues of <em>P. vannamei</em> fed with FD-LAB supplemented diet. This study demonstrated how FD-LAB can interfere with the virulence and pathogenicity of <em>V. parahaemolyticus</em>, as well as its influence in shaping the gill and gut bacterial communities of <em>P. vannamei</em>. These findings reinforce the potential of FD-LAB as a dietary supplement for shrimp.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742092"},"PeriodicalIF":3.9,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of trimethylamine N-oxide supplementation on low-salinity tolerance of the mud crab Scylla paramamosain relative to Na+/K+-ATPase activity and haemolymph osmolality","authors":"Jiaxin Jiang ,&nbsp;Runze Jin ,&nbsp;Ce Shi ,&nbsp;Yangfang Ye ,&nbsp;Shujian Chen ,&nbsp;Changkao Mu ,&nbsp;Ronghua Li ,&nbsp;Lei Liu ,&nbsp;Xiaopeng Wang ,&nbsp;Yueyue Zhou ,&nbsp;Chunlin Wang","doi":"10.1016/j.aquaculture.2024.742096","DOIUrl":"10.1016/j.aquaculture.2024.742096","url":null,"abstract":"<div><div>Mud crabs (<em>Scylla paramamosain</em>) are important marine organisms. Typhoons and other events lead to sudden decreases in salinity, which substantially influence the survival and growth of mud crabs. This study aimed to enhance the low-salinity tolerance capacity of mud crabs through nutritional regulation of trimethylamine N-oxide (TMAO). A seven-day experiment explored the impact of dietary supplementation with five concentrations of TMAO, 0 mg/kg (CK), 100 mg/kg (T1), 200 mg/kg (T2), 400 mg/kg (T4), and 800 mg/kg (T8), on the survival rate and physiological response of juvenile mud crabs under acute low-salinity conditions. The results showed that a diet supplemented with TMAO significantly impacted the survival rate, structural integrity of the gill tissue, osmolality of haemolymph, ion content (Na⁺, Cl⁻, K⁺, and Ca²⁺), activity of Na⁺/K⁺-ATPase, and gene expression of crucial transporters and enzymes (such as <em>nkcc</em>, <em>nka</em>, <em>clc</em>, and <em>gdh</em>) in crabs following acute exposure to low-salinity conditions. In particular, 400 mg/kg TMAO supplementation, resulted in a significant improvement in the survival rate of crabs after 168 h of low-salinity exposure, and a survival rate of 89 %, in comparison to the CK group, which only achieved a survival rate of 27 % (<em>P</em> &lt; 0.01). Additionally, 400 mg/kg TMAO supplementation mitigated damage to posterior gill tissue during low-salinity stress, with no observed difference in gill filament diameter before and after low-salinity stress. Furthermore, crabs treated with TMAO manifested enhanced haemolymph osmolality, increased Na⁺/K⁺-ATPase activity in the posterior gills, increased TMAO accumulation within the haemolymph and muscle tissues, and upregulated gene expression associated with ion transporters and enzymes under low salinity. Overall, these results suggest that dietary supplementation with 400 mg/kg TMAO increases the survival rate of mud crabs when acutely exposed to low-salinity stress.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742096"},"PeriodicalIF":3.9,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological and transcriptome analysis of gonadal development provides new insights into the characteristics of sex differentiation and the screening of key genes in the golden pompano (Trachinotus blochii)","authors":"Feibiao Song ,&nbsp;Huan Liang ,&nbsp;Zhi Li ,&nbsp;Da Zheng ,&nbsp;Huapeng Chen ,&nbsp;Xinxin Wang ,&nbsp;Chaoyue Deng ,&nbsp;Wenyan Lin ,&nbsp;Changlin Chen ,&nbsp;Junlong Sun ,&nbsp;Li Zhou ,&nbsp;Jian Luo","doi":"10.1016/j.aquaculture.2024.742083","DOIUrl":"10.1016/j.aquaculture.2024.742083","url":null,"abstract":"&lt;div&gt;&lt;div&gt;&lt;em&gt;Trachinotus blochii&lt;/em&gt; is one of the important mariculture fish species in China and is a key driver of offshore aquaculture development. The growth of golden pompano is sexually dimorphic, with females growing faster than males, suggesting that monosex farming could significantly improve economic efficiency. Therefore, it is crucial to study the early gonadal development, differentiation characteristics, and the regulatory genes involved to understanding the molecular mechanisms of sex determination and differentiation in &lt;em&gt;T. blochii&lt;/em&gt;. In this study, juvenile &lt;em&gt;T. blochii&lt;/em&gt; were sampled continuously from 0 to 186 days post-hatching (dph). Determine its genetic sex through sex molecular markers, and histological sections were used to observe the gonadal development of males and females at different ages. Transcriptomic analysis of the gonads at different stages of sex differentiation was also conducted to screen for sex-determining genes. The results showed that a single primordial germ cell (PGC) first appeared on one side of the primordial gonad at 8 dph. At 12 dph, a small number of somatic cells surrounded the PGCs, indicating the gonadal primordium had formed. By 15 dph, a single layer of somatic cells completely enveloped the PGCs, forming the primordial gonad. At 41 dph, a narrow slit-like ovarian cavity was observed in the gonadal section, marking the beginning of ovarian development. At 78 dph, a distinct testicular primordium was observed, signaling the onset of testicular development. By 105 dph, a small number of oocytes were present, indicating cytological ovarian differentiation. By 186 dph, a large number of primary spermatocytes and a small number of secondary spermatocytes were present, marking cytological testicular differentiation. In summary, based on histology, ovarian differentiation in &lt;em&gt;T. blochii&lt;/em&gt; occurred at 41 dph, while testicular differentiation occurred at 78 dph. Transcriptomics revealed a total of 22,278 genes across 36 cDNA libraries, with 125, 119, 191, 130, 119, and 1745 differentially expressed genes (DEGs) at the six respective developmental stages (10, 20, 30, 45, 60, and 90 dph). Enrichment analysis identified several GO terms significantly associated with sex, including follicle development, regulation of oogenesis, reproductive development, sperm motility, and oocyte maturation. KEGG enrichment revealed key pathways related to gonadal development and sex differentiation, such as estrogen signaling, ovarian steroidogenesis, steroid hormone biosynthesis, progesterone-mediated oocyte maturation, and oocyte meiosis. From the DEGs related to sex, 100 genes were screened through GO and KEGG enrichment analyses. Protein-protein interaction network analysis indicated that genes such as &lt;em&gt;cyp19a1&lt;/em&gt;, &lt;em&gt;hsd17β7&lt;/em&gt;, &lt;em&gt;hsd17β1&lt;/em&gt;, &lt;em&gt;hsd17β3&lt;/em&gt;, &lt;em&gt;hsd3β&lt;/em&gt;, &lt;em&gt;ugt1a1&lt;/em&gt;, &lt;em&gt;hsd17β12b&lt;/em&gt;, &lt;em&gt;cyp7a1&lt;/em&gt;, &lt;em&gt;esr2&lt;/em&gt;, and &lt;em&gt;bmp15&lt;/em&gt; play important roles i","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742083"},"PeriodicalIF":3.9,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Genetic diversity study of self-fertilized and gynogenetic Japanese flounder (Paralichthys olivaceus) in whole genomic level” [Aquaculture, Volume 598, 15 March 2025, 742019]","authors":"Lize San ,&nbsp;Guixing Wang ,&nbsp;Xiaoyan Zhang ,&nbsp;Yitong Zhang ,&nbsp;Wei Cao ,&nbsp;Zhongwei He ,&nbsp;Yufeng Liu ,&nbsp;Yucong Yang ,&nbsp;Mingyang Liu ,&nbsp;Yuqin Ren ,&nbsp;Yufen Wang ,&nbsp;Jilun Hou","doi":"10.1016/j.aquaculture.2024.742057","DOIUrl":"10.1016/j.aquaculture.2024.742057","url":null,"abstract":"","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742057"},"PeriodicalIF":3.9,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of two runt genes (MnRunt1, MnRunt2) and their regulation of prophenoloxidase-related genes expression in Macrobrachium nipponense","authors":"Qian Ren ,&nbsp;Ying Huang ,&nbsp;Wei Qin","doi":"10.1016/j.aquaculture.2024.742086","DOIUrl":"10.1016/j.aquaculture.2024.742086","url":null,"abstract":"<div><div>Runt-related transcription factors (RUNX) exist in vertebrates and invertebrates and play critical roles in development, metabolism, and diseases. Runt is a kind of RUNX. In this study, two <em>Runt</em> genes were cloned from the oriental river prawn <em>Macrobrachium nipponense</em>, named <em>MnRunt1</em> and <em>MnRunt2</em>. The coding region of <em>MnRunt1</em> is 1302 bp, encoding a protein of 433 amino acids. The full-length cDNA sequence of the <em>MnRunt2</em> gene is 2498 bp, including an open reading frame of 1536 bp encoding 511 amino acids. <em>MnRunt2</em> produces another seven isoforms by alternative splicing. MnRunt1 and MnRunt2 both contain a Runt domain. Phylogenetic tree analysis showed that MnRunt1 and MnRunt2 are located in two different clades. <em>MnRunt1</em> and <em>MnRunt2</em> are widely distributed in all tested tissues and highly expressed in the hepatopancreas of <em>M. nipponense</em>. The expression levels of <em>MnRunt1</em> and <em>MnRunt2</em> in the hemocytes of <em>M. nipponense</em> are upregulated at various levels at multiple time points after infection with <em>Vibrio parahaemolyticus</em> or <em>Staphylococcus aureus</em>. The expression levels of <em>clip domain serine protease 1</em> (<em>MncSP1</em>), <em>MncSP1-isoform</em>, <em>MncSP2</em>, <em>SP homolog</em> (<em>MnSPH</em>), and <em>phenoloxidase</em> (<em>MnPO</em>) are inhibited in <em>MnRunt1</em> or <em>MnRunt2</em> knockdown prawns with <em>V. parahaemolyticus</em> or <em>S. aureus</em> challenge. Moreover, the survival rate of prawns is significantly reduced after <em>MnRunt1</em> or <em>MnRunt2</em> knockdown. Therefore, it can be speculated that <em>MnRunt1</em> and <em>MnRunt2</em> are involved in the innate immune defense of <em>M. nipponense</em> by regulating the expression of genes related to the prophenoloxidase pathway.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742086"},"PeriodicalIF":3.9,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term trends in the economic viability of pond aquaculture in Central Europe – The example of Czechia","authors":"Radek Zdeněk ,&nbsp;Jana Lososová ,&nbsp;Jan Mráz","doi":"10.1016/j.aquaculture.2024.742069","DOIUrl":"10.1016/j.aquaculture.2024.742069","url":null,"abstract":"<div><div>In Czechia, aquaculture is one of the smallest sectors in terms of employment and added value. However, it is of considerable importance, primarily because of the ecosystem services it provides and the beneficial effects of consuming fish products on human health. This paper aims to assess the economic viability of Czech aquaculture, specifically by evaluating the development of employment, production, and labour productivity in the aquaculture sector from 1993 to the present, in comparison with agriculture and the overall economy. The production of Czech aquaculture depends on the production of common carp in ponds and the conditions for its monetisation. A fundamental factor is the price of common carp, which has remained almost constant over the last two decades. The consequence of this is stagnating production, with labour productivity lagging behind other sectors. Aquaculture companies are not competitive in the labour market and must contend with the outflow of qualified workers. Current trends could lead to the liquidation of aquaculture companies and the deterioration of production ponds, with all the negative implications that this entails.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742069"},"PeriodicalIF":3.9,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of genetic parameters for upper thermal tolerance and growth traits in grass carp (Ctenopharyngodon idella)","authors":"Heping Tang ,&nbsp;Wei Zhang ,&nbsp;Chendong Tao ,&nbsp;Chenyang Zhang ,&nbsp;Lang Gui ,&nbsp;Nan Xie ,&nbsp;Xiaoyan Xu ,&nbsp;Jiale Li ,&nbsp;Yubang Shen","doi":"10.1016/j.aquaculture.2024.742073","DOIUrl":"10.1016/j.aquaculture.2024.742073","url":null,"abstract":"<div><div>As an ectothermic animal that lives in water for a lifetime, grass carp (<em>Ctenopharyngodon idella</em>) has a strong dependence on environmental temperature. The objective of this study was to estimate the genetic parameters for heat tolerance and growth traits in grass carp using reconstructed pedigree and molecular relatedness. The experimental population consisted of 800 offspring at 60 days old and 55 parents (♀, 32; ♂, 23) from selected line F4 and 539 at 6 months old offspring from selected line F5 with 87 parents (♀, 44; ♂, 43). Genetic genotyping of the experimental population was conducted using 10 pairs of microsatellite markers, and all 10 markers exhibited a high degree of genetic polymorphism across all 1394 grass carp (<em>H</em>_<em>o</em> = 0.751–0.981; <em>H</em>_<em>e</em> = 0.783–0.942; <em>PIC</em> = 0.759–0.938). Parentage assignment divided F4 individuals into 203 full-sib, and the Pearson correlation coefficient between reconstructed pedigree relatedness and molecular relatedness was 0.475. For the selected line F4, the heritability for each trait based on reconstructed pedigree relatedness (PR) were 0.320 ± 0.092 for body weight (BW), 0.303 ± 0.093 for standard length (SL), 0.246 ± 0.082 for body height (BH), 0.193 ± 0.074 for body thickness (BT), and 0.131 ± 0.063 for upper thermal tolerance (UTT) was 0.131 ± 0.063. Similarly, the heritability for each trait based on molecular relatedness (MR) were 0.330 ± 0.092 for body weight (BW), 0.348 ± 0.097 for standard length (SL), 0.248 ± 0.080 for body height (BH), 0.197 ± 0.073 for body thickness (BT) and the heritability for upper thermal tolerance (UTT) was 0.260 ± 0.037. For the F5 generation, the heritability of body weight (BW) and standard length (SL) estimated by molecular relatedness (MR) was 0.281 ± 0.045 and 0.283 ± 0.044. These results indicate that molecular relatedness is a viable method for estimating genetic parameters when pedigree information is inaccurate or missing. The findings of this study can provide theoretical support for the optimization of breeding programs aimed at developing productive and heat tolerant grass carp strains.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742073"},"PeriodicalIF":3.9,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosome-mediated ACSL4 mRNA delivery suppresses WSSV infection by promoting ferroptosis in mud crab","authors":"Xinshan Zhao ,&nbsp;Mingming Sun ,&nbsp;Yi Gong","doi":"10.1016/j.aquaculture.2024.742078","DOIUrl":"10.1016/j.aquaculture.2024.742078","url":null,"abstract":"<div><div>As a novel immunomodulator, exosomes can deliver molecules such as mRNA and proteins between cells, providing resistance against pathogen infections. ACSL4, a lipid metabolizing enzyme, has been extensively studied as a well-established regulator of ferroptosis in the immune defense mechanism of vertebrates. However, the role of exosomes and ACSL4-mediated ferroptosis in the antiviral immune response has not been addressed in invertebrates. This study found that exosomes secreted by WSSV-infected mud crabs (<em>Scylla paramamosain</em>) can inhibit viral replication by activating ferroptosis. In addition, ACSL4 mRNA was found to be more packaged in exosomes secreted by hemocytes during viral infection in mud crabs. Then, the role of exosome-mediated ACSL4 mRNA in antiviral immunomodulation was further explored by RNAi methods. The results showed that WSSV-infected exosomes encapsulated with more <em>Sp</em>ACSL4 mRNA could lead to increased Fe²⁺, reactive oxygen species (ROS), and malondialdehyde (MDA) contents, as well as decreased GPX4 expression, so as to activate ferroptosis and further suppress WSSV replication in mud crabs. This study provides novel insights into the innate defense mechanisms of crustaceans.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742078"},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into feeding behavior and digestive physiology of the Chinese mitten crab (Eriocheir sinensis) zoea 5 (Z5) and megalopa (M)","authors":"Cheng-Yi Weng ,&nbsp;Jian-Yang Sun ,&nbsp;Yu-Chen Chen ,&nbsp;Yu-Hai You ,&nbsp;Ji-Tao Zheng ,&nbsp;Shamoona Arshad ,&nbsp;Yong-Xu Cheng ,&nbsp;Xiao-Zhen Yang","doi":"10.1016/j.aquaculture.2024.742037","DOIUrl":"10.1016/j.aquaculture.2024.742037","url":null,"abstract":"<div><div>The metamorphosis from zoea 5 (Z5) to megalopa (M) stage represents a critical transit in the larval development of the Chinese mitten crab (<em>Eriocheir sinensis</em>). This study investigated the feeding behaviors and digestive physiology of Z5 and M stages through behavioral, morphological, and physiological analyses. The results of feeding behavior have showed: 1) M stage had significantly higher average swimming speed (3.42 ± 0.22 cm/s) than Z5 (1.22 ± 0.15 cm/s) at 07:00 am, 12:00 pm, and 17:00 pm (<em>p</em> &lt; 0.05). 2) Only M stage preferred <em>Artemia salina</em> in mixed feed trials. 3) M exhibited a distinct feeding rhythm with intake at 07:00 am and 17:00 pm being 1.15 and 1.21 times higher than at 12:00 pm, respectively (<em>p</em> &lt; 0.05). 4) M consumed approximately 1.8 times more food than Z5 (<em>p</em> &lt; 0.05). The results of digestive physiology have showed: 1) Food accumulation in the hepatopancreas peaked at 60 min in Z5 and 120 min in M by situ observations of accumulation in the hepatopancreas after ingesting red algal powder, with faster food transit time in Z5 (25–50 min) compared to M (80–120 min) (<em>p</em> &lt; 0.05). 2) Significant rhythmic changes in digestive enzyme activities (amylase, trypsin, lipase) and gene expression were observed during the Z5 and M stages, with peak levels at 07:00 am and 17:00 pm for most enzymes. These findings provide crucial theoretical insights for optimizing feeding strategies and enhancing the healthy cultivation of <em>E.sinensis</em> larvae, potentially leading to improved yield and economic value of megalopae in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742037"},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aquaculture sector in Colombia: Uncovering sustainability, transformative potential, and trends through bibliometric and patent analysis","authors":"Luis Andrés Leal,&nbsp;Astrid C. Ángel-Ospina,&nbsp;José Antonio Lara Ramos,&nbsp;Fíderman Machuca-Martínez","doi":"10.1016/j.aquaculture.2024.742068","DOIUrl":"10.1016/j.aquaculture.2024.742068","url":null,"abstract":"<div><div>This research presents a bibliometric and patent analysis of the development of aquaculture in Colombia. An exhaustive search for data and patents specifically related to aquaculture and aquaponics in Colombia was conducted, excluding non-relevant data. Databases such as Scopus, Web of Science, SCIELO, ProQuest, and Orbit Intelligence were used. Notably, the industry has experienced consistent growth, driven by genetic improvements, innovations in production systems, and the optimization of growing conditions. However, challenges persist, including limited access to quality water and inadequate wastewater treatment. This situation generates concern about food security. The analysis reveals that the National University of Colombia leads in publications on aquaponics, while the University of Magdalena leads collaboration networks in the country. In contrast to these advances, private sector participation is still low in research and development (R&amp;D) in aquaponics, indicating the need for greater integration between the public and private sectors. Finally, significant progress has been made in aquaponics, but Colombia still faces very important challenges in terms of water treatment and private industry participation in R&amp;D. Therefore, it is important to address these challenges comprehensively to guarantee sustainable development in Colombian aquaculture and in other regions of Latin America.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742068"},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}