Aquaculture最新文献

{"title":"Comparative study on the role of various sterols in enhancing ovarian development and metabolic functions in Pacific white shrimp (Litopenaeus vannamei) broodstock","authors":"Xiaolong Liang ,&nbsp;Xianming Xiao ,&nbsp;Xiuju Gao ,&nbsp;Muchun Wen ,&nbsp;Yongqiang Song ,&nbsp;Chang Xu ,&nbsp;Fenglu Han ,&nbsp;Erchao Li","doi":"10.1016/j.aquaculture.2024.741934","DOIUrl":"10.1016/j.aquaculture.2024.741934","url":null,"abstract":"<div><div>In this study, the Pacific white shrimp (<em>Litopenaeus vannamei</em>) broodstock was considered to rely on dietary sterols for ovarian development because of the inability of the animals to synthesize sterols endogenously. Therefore, we investigated the effects of cholesterol, ergosterol, β-sitosterol, and fucosterol as sterol sources, compared with a control group lacking sterols, on the growth, reproduction, and metabolism of shrimp for a 28-day feeding trial, with an average weight of 32.28 ± 2.00 g in female L. <em>vannamei</em> broodstock. The results demonstrated that cholesterol supplementation significantly enhanced shrimp growth, the gonadosomatic index, and yolk particle deposition, outperforming the other sterol groups and the control group. Notably, while broodstock in the ergosterol group presented the greatest weight gain and yolk granule deposition, they did not achieve the same level of gonadal development or metabolic efficiency as those receiving cholesterol. Additionally, the β-sitosterol group demonstrated a favorable hepatosomatic index but still fell short of the benefits provided by cholesterol. Cholesterol has unique advantages in promoting growth and ovarian development, which may be attributed to its more efficient metabolic utilization and enhanced hormone synthesis, as indicated by the significantly higher serum levels of 17β-estradiol and progesterone in the cholesterol group. Furthermore, cholesterol supplementation significantly upregulated vitellogenin mRNA expression in the hepatopancreas and ovaries, thereby promoting ovarian maturation. Additionally, γ-linolenic acid and 9,10-epoxyoctadecenoic acid were significantly upregulated in the cholesterol group, facilitating ovarian lipid accumulation. In conclusion, the use of cholesterol as a sterol source in the diet significantly promoted the growth and ovarian development of L. <em>vannamei</em> by regulating lipid metabolism and hormone synthesis.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"597 ","pages":"Article 741934"},"PeriodicalIF":3.9,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recirculating aquaculture mode has high sustainability and environmental accommodation capacity via better energy-saving and water-saving effects in Cynoglossus semilaevis production","authors":"Feng Wang ,&nbsp;Xiao Mei ,&nbsp;Bo Liu ,&nbsp;YuMing Zhao ,&nbsp;Juan Li ,&nbsp;WenSheng Li ,&nbsp;Shiyong Chen","doi":"10.1016/j.aquaculture.2024.741904","DOIUrl":"10.1016/j.aquaculture.2024.741904","url":null,"abstract":"<div><div>Recirculating aquaculture mode is a promising direction for future aquaculture development due to its energy, water, and land-saving advantages, high efficiency, and lack of seasonal restrictions. This study evaluated and compared the economic efficiency and environmental sustainability of <em>Cynoglossus semilaevis</em> production in flow-through and recirculating aquaculture modes using the economic methodology and the emergy analysis method. The results showed that: 1) The fixed investment cost for the recirculating aquaculture system was 2.188 times as that of the flow-through aquaculture system. 2) The recirculating water aquaculture mode saved 54.55 % of electricity, 93 % of coal, and 95 % of water compared with the flow-through aquaculture mode. 3) The recirculating aquaculture mode performed better in all breeding parameters than the flow-through aquaculture mode. In addition, the yield, tax revenue, and profit of the recirculating aquaculture system were 1.71 times, 1.71 times, and 2.98 times that of the flow-through aquaculture mode, respectively, and the break-even point was 4.35 %, indicating that the former has better risk resistance ability compared to the latter. 4) For the recirculating aquaculture mode the renewable energy value consumption was only 3.272 % that of the flow-through aquaculture mode, the non-renewable energy value input was 1.59 times that of the flow-through aquaculture mode, and the output energy value was 1.71 times that of the flow-through aquaculture mode. 5) The EIR value of the recirculating aquaculture mode was 48.89 times that of the flow-through aquaculture mode, and the EYR value was 1.069 times that of the flow-through aquaculture mode, which indicated that the recirculating aquaculture mode was less dependent on the environment and had higher production efficiency. 6) The flow-through aquaculture mode used 34.77 % of the environmental energy value input for aquaculture production. However, the uncontrolled use of groundwater and geothermal resources had caused great damage to the environmental ecosystem. To sum up, the recirculating aquaculture mode had higher sustainability and environmental accommodation capacity and could significantly reduce the pressure of aquaculture industry on the environment.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"597 ","pages":"Article 741904"},"PeriodicalIF":3.9,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142756973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blunt snout bream (Megalobrama amblycephala) circRXRBB regulates antimicrobial immune response against Aeromonas hydrophila infection via miR-155/socs1a axis","authors":"Wenya Zhai ,&nbsp;Junzhao Lin ,&nbsp;Zexia Gao ,&nbsp;Huanling Wang ,&nbsp;Hong Liu","doi":"10.1016/j.aquaculture.2024.741899","DOIUrl":"10.1016/j.aquaculture.2024.741899","url":null,"abstract":"<div><div>Circular RNAs (circRNAs) play an important role in various of human diseases by acting as competing endogenous RNAs (ceRNAs) and can serve as biomarkers and therapeutic targets. Our prior research has demonstrated that the circRNA-miRNA-mRNA regulatory network plays an important role in blunt snout bream (<em>Megalobrama amblycephala</em>) resistance to <em>Aeromonas hydrophila</em> infection<em>.</em> However, the exact regulatory mechanisms still need to be further explored. In this research, we identified a circRNA called circRXRBB that was down regulated following <em>A. hydrophila</em> challenge, suggesting its possible involvement in antimicrobial immune. Subsequently, the present study demonstrated that circRXRBB overexpression suppressed the expression levels of pro-inflammatory factors (<em>il-6, il-1β, tnf-α</em>, and <em>ifn-γ</em>). Dual luciferase assay demonstrated that circRXRBB could directly adsorb miR-155 through sponge action, while miR-155 mimic could restore the inhibitory effect on inflammatory factors caused by circRXRBB overexpression. In addition, mir-155 can target <em>socs1a</em>, and the mir-155/<em>socs1a</em> axis is one of the important pathways for circRXRBB to regulate immune response. In conclusion, this research demonstrated that circRXRBB is critical in regulating the antibacterial defense against <em>A. hydrophila</em> infection, which contributes to a better understanding of the ceRNA regulatory network and offers a foundation for future strategies in fish disease diagnose, prevention, and treatment.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"597 ","pages":"Article 741899"},"PeriodicalIF":3.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification, evolution, diversity and expression of Caspase gene family in Haliotis discus hannai","authors":"Yuping Chen ,&nbsp;Wangying Wu ,&nbsp;Hui Ge ,&nbsp;Zhen Zhao ,&nbsp;Hanling Gan ,&nbsp;Nan Chen ,&nbsp;Jianpeng Huang ,&nbsp;Chao Fan ,&nbsp;Mingyi Cai ,&nbsp;Yilei Wang ,&nbsp;Ziping Zhang","doi":"10.1016/j.aquaculture.2024.741900","DOIUrl":"10.1016/j.aquaculture.2024.741900","url":null,"abstract":"<div><div>Apoptosis is crucial in the immune defense mechanisms of mollusks. In this study, 25 <em>Caspase</em> genes were identified from <em>Haliotis discus hannai’</em> genome. Through structural and evolutionary relationship analysis, highly conserved data were observed between <em>H. discus hannai</em> and other mollusks in the Caspase. Notably, the first discovery of Caspase-9 in abalones in this study. Collinearity analysis detected numerous tandem duplication events within the <em>Caspase</em> genes, implicating these duplications as the principal evolutionary driver of the Caspase family in <em>H. discus hannai</em>. Sequence diversity analysis found 216 missense mutation sites in <em>Caspase</em> genes of <em>H. discus hannai</em>. Alternative splicing analysis revealed multiple splicing sites and events in <em>Caspase</em> of <em>H. discus hannai</em>. Additionally, differential alternative splicing analysis showed the significant induction of <em>Caspase-2</em>, <em>3</em>, and <em>8</em> variants following <em>Vibrio</em> infection. Transcriptome quantitative and qRT-PCR demonstrated that most <em>Caspase</em> genes in <em>H. discus hannai</em> respond to heat and <em>Vibrio</em> inoculation. Our findings offer novel perspectives on Caspase function and immune modulation mechanisms in <em>H. discus hannai</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 741900"},"PeriodicalIF":3.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary resveratrol supplementation on the growth performance, physiological health, transcriptomic response, and intestinal microflora of Litopenaeus vannamei","authors":"Zhibin Fu ,&nbsp;Jian Chen ,&nbsp;Hang Yuan ,&nbsp;Yudong Zheng ,&nbsp;Gongyu Li ,&nbsp;Sheng Ke ,&nbsp;Shuang Zhang ,&nbsp;Lili Shi","doi":"10.1016/j.aquaculture.2024.741927","DOIUrl":"10.1016/j.aquaculture.2024.741927","url":null,"abstract":"<div><div>We evaluated the effects of resveratrol (RES) supplementation on the growth performance, physiological health, transcriptomic response, and intestinal health of <em>Litopenaeus vannamei</em>. <em>L. vannamei</em> (initial body weight: 0.40 ± 0.02 g) were fed using six types of experimental diets (R0, R50, R100, R200, R400, and R800, supplemented with 0, 50, 100, 200, 400 and 800 mg/kg of RES, respectively) for 56 days. The results showed that dietary RES had no significant effect on the growth of L. <em>vannamei</em>. The survival rate (SR) of shrimp in the R400 group was significantly higher than that in the R0 group after exposure to <em>Vibrio parahaemolyticus</em>. RES supplementation dramatically enhanced the immunity of L. <em>vannamei</em>, as indicated by the increased serum levels of important indicators of immune function. The activities of intestinal digestive enzymes increased dramatically with increasing RES supplementation. Transcriptome analysis revealed that adding RES had a considerable effect on various metabolic and immune-related pathways in L. <em>vannamei</em>, including beta-alanine metabolism, ascorbate and aldarate metabolism, and drug metabolism-other enzymes. 16S rDNA analysis indicated that RES supplementation improved the intestinal microflora composition of L. <em>vannamei</em> by enhancing the abundance of beneficial bacteria and reducing the abundance of harmful bacteria. In conclusion, this study indicated that RES supplementation at an appropriate dose (400 mg/kg) could improve the physiological health, regulate the response at the transcriptional level, and ameliorate the intestinal microflora structure of L. <em>vannamei</em>. These results offer novel ideas and theoretical support for using RES in L. <em>vannamei</em> cultures as an immune-boosting supplement.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"597 ","pages":"Article 741927"},"PeriodicalIF":3.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A holo-omics analysis shows how sugar kelp can boost gut health in Atlantic salmon","authors":"Jacob A. Rasmussen ,&nbsp;Annette Bernhard ,&nbsp;Laurène A. Lecaudey ,&nbsp;Varsha Kale ,&nbsp;Germana Baldi ,&nbsp;Lene S. Myrmel ,&nbsp;Martin Hansen ,&nbsp;Jaelle C. Brealey ,&nbsp;Sarah S.T. Mak ,&nbsp;Ida B. Nielsen ,&nbsp;Lorna Richardson ,&nbsp;Lise Madsen ,&nbsp;Karsten Kristiansen ,&nbsp;M. Thomas P. Gilbert ,&nbsp;Michael D. Martin ,&nbsp;Robert D. Finn ,&nbsp;Harald Sveier ,&nbsp;Morten T. Limborg","doi":"10.1016/j.aquaculture.2024.741913","DOIUrl":"10.1016/j.aquaculture.2024.741913","url":null,"abstract":"<div><div>The projected growth of Aquaculture requires continuous optimisation of feeding regimes, especially in enhancing disease resilience through immunostimulating feed supplements. Seaweed has been introduced as a promising supplement due to its proposed immunostimulant effects, while mitigating carbon emissions and marine eutrophication. Two separate growth trials were performed to evaluate the effects of a seaweed supplement, focusing on a closed land-based RAS system with fish growing from ~300–600 g and an open water net-based trial with fish growing from ~500–2500 g. A multi-omics approach was taken to investigate the effect of using up to 2 % fermented sugar kelp (<em>Saccharina latissima</em>) as a supplement on the growth performance and gut health of Atlantic salmon (<em>Salmo salar</em> L.). Standard growth metrics and quality indicators were coupled with multi-omics data for the host intestinal transcriptome, intestinal metabolome, and intestinal microbiome. Levels of iodine and heavy metals in the fish fillets resulting from seaweed inclusion were evaluated. Seaweed additives up to 2 % had no negative impact on the salmon growth performance or other health parameters. While concentration of iodine increased with seaweed addition, all levels remain below recommended thresholds. Furthermore, gut transcriptome analyses of 179 individuals revealed multiple indications for an immunostimulant effect of seaweed, including overexpression of the mucin biosynthesis and pyrimidine metabolism pathways related to mucus production and intestinal homeostasis. Genome-resolved metagenomics revealed a core microbiome dominated by <em>Mycoplasma</em>, <em>Photobacterium</em>, and <em>Brevinema</em> species. Interestingly, the assembled genomes of these three core species had consistently higher levels of genome erosion and gene loss compared to other present species suggesting host adaptation. Further, reduced prevalence of known pathogens such as Aliivibrio highlights a potential protective effect of seaweed by impeding growth of opportunistic pathogens. Results suggest that supplementation of up to 2 % fermented seaweed can increase feed efficiency, stabilise the intestinal microbiome and potentially increase resistance against known pathogenic bacteria including Aliivibrio species.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"597 ","pages":"Article 741913"},"PeriodicalIF":3.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of economic and ecological benefits between factory water exchange model and biofloc model based on meta analysis","authors":"Yuzhen Wang ,&nbsp;Zhao Chen ,&nbsp;Zhiqiang Chang ,&nbsp;Shuangyong Zhang ,&nbsp;Gao Meng ,&nbsp;Jian Li","doi":"10.1016/j.aquaculture.2024.741907","DOIUrl":"10.1016/j.aquaculture.2024.741907","url":null,"abstract":"<div><div>Studies on aquaculture models currently focus on assessing single economic or ecological benefits, while the analysis of the combined economic and ecological benefits of different factory farming models has rarely been reported. Furthermore, there is a lack of comprehensive research on the combined effects of multiple farming models on the environment. Therefore, we used Meta-analysis to quantify and compare the economic and ecological benefits of different factory farming modes and their combinations under multidimensional conditions, including the Indoor super-intensive shrimp culture model (ISIC-S), Indoor super intensive biofloc shrimp culture (ISBIC-S), Indoor super-intensive recirculating shrimp culture (ISIRC-S), and the Indoor super-intensive fish culture model (ISIC-F). The results showed that the economic and ecological benefits of using a combination of multiple factory farming models were significantly increased by 38 % compared to a single factory farming model, with an 11 % increase in economic benefits and an 88 % increase in ecological benefits in a single benefit. Specifically, the economic and ecological benefits increased by 64.65 % for the combination of ISBIC-S, ISIRC-S and ISIC-F modes compared to ISIC-S. The combination of ISIRC-S and ISIC-F increased the economic and ecological benefits by 13.75 % compared to ISBIC-S. In terms of economic benefits, the cost structure of the four factory farming models was similar due to the similar farming technology, and the total cost of the combination of multiple factory farming models increased by only 6 %. However, due to the maturity of the farming technology, the ISIC-S model had the highest net profit of 182,778.75US$ ha⁻¹ as compared to the single model, which was better in terms of economic benefits. Regarding ecological benefits, the multiple factory model showed significant reduction in suspended solids compared to the single model. The analysis of bacterial diversity and carbon sink capacity indicated that the bacterial diversity and abundance in ISBIC-S and ISIRC-S modes were higher than that of ISIC-S, and the carbon emission per unit of production was highest in ISIC-F (1858.14 g kg⁻¹CO₂), followed by ISBIC-S (1732.66 kg⁻¹CO₂). In conclusion, adopting multiple synergistic factory farming models is a feasible and sustainable approach to enhancing economic returns while significantly improving the ecological environment. These findings provide a theoretical foundation for developing factory farming models that promote the sustainable development of aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"597 ","pages":"Article 741907"},"PeriodicalIF":3.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into ER stress mediated by ATF6 and IRE1-XBP1 signals in yellow catfish under hypoxia","authors":"Ying Wan ,&nbsp;Haokun Liu ,&nbsp;Junyan Jin ,&nbsp;Zhimin Zhang ,&nbsp;Yunxia Yang ,&nbsp;Xiaoming Zhu ,&nbsp;Shouqi Xie ,&nbsp;Dong Han","doi":"10.1016/j.aquaculture.2024.741926","DOIUrl":"10.1016/j.aquaculture.2024.741926","url":null,"abstract":"<div><div>In aquaculture, fish are often exposed to the major stressor of hypoxia. However, the mechanism of hypoxia-induced stress remains unclear. The present study aimed to investigate the physiological and molecular responses of yellow catfish (<em>Pelteobagrus fulvidraco</em>) to hypoxia stress and to further elucidate its stress response mechanisms. Each fish (28.14 ± 0.75 g) in the hypoxia group was individually placed in a 1 L transparent plastic tank and transferred to a closed hypoxia environment, where the oxygen and carbon dioxide levels were adjusted to 2 % and 0.5 %, respectively. The results showed that hypoxia stress induced a stress response in yellow catfish, characterized by significant changes in lactate dehydrogenase (LD) and cortisol levels in plasma and liver, as well as increased apoptosis of brain cells and liver damage. Additionally, the HPA axis was activated, playing a crucial role in stress regulation during hypoxia exposure. And under hypoxia conditions, endoplasmic reticulum (ER) stress pathways were activated in yellow catfish liver, specifically showing significant upregulation of the activating transcription factor 6 (ATF6) and inositol-requiring enzyme 1 (IRE1)-X-box binding protein 1 (XBP1) signals, while the activated transcription factor 4 (ATF4) pathway remained inactive. The expression levels of <em>atf6</em>, <em>ire1</em> and <em>xbp1</em> genes, as well as ATF6, IRE1 and XBP1 proteins in the ATF6 and IRE1-XBP1 pathways, were significantly elevated. However, there was no significant differences in the expression levels of protein kinase R-like ER kinase (<em>perk</em>), eukaryotic translation initiation factor 2α (<em>eif2α</em>) and <em>atf4</em> key genes, as well as PERK, EIF2α and ATF4 proteins in the PERK-ATF4 pathway. It is concluded for the first time that hypoxia significantly activates ER stress through the ATF6 and IRE1-XBP1 signaling pathways, but not the PERK-ATF4 pathway in yellow catfish. These findings not only provide new insights into the stress response mechanisms of fish but also offer the accurate molecular target of hypoxia-induced stress for aquaculture management.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"597 ","pages":"Article 741926"},"PeriodicalIF":3.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Largemouth bronze gudgeon (Coreius guichienoti) responds positively to liver damage induced by high feeding rates via enhancing self-repair capability","authors":"Pei Chen ,&nbsp;Xiaofang Liang ,&nbsp;Wei Jiang ,&nbsp;Jing Yang ,&nbsp;Yu Zhao ,&nbsp;Chao Lei ,&nbsp;Huantao Qu","doi":"10.1016/j.aquaculture.2024.741919","DOIUrl":"10.1016/j.aquaculture.2024.741919","url":null,"abstract":"<div><div>Overfeeding intermittently induces metabolic liver diseases in aquaculture practice. Currently, less information regarding the impact of feeding rates on hepatic lipid metabolism was reported. Therefore, this study investigated the effects of feeding rates (2 %, 3 %, 4 %, and 5 % of body weight per day) on growth performance, hepatic lipid metabolism, immunity, inflammation, and apoptosis in juvenile largemouth bronze gudgeon (<em>Coreius guichenoti</em>) over 8 weeks. The results demonstrated that increasing feeding rates significantly enhanced weight gain rate and specific growth rate. Furthermore, the lipid content of the whole body increased progressively, with the highest levels observed in the 5 % group. High feeding rates also resulted in significant increases in hepatic TG, TC, and NEFA levels. This was accompanied by upregulated mRNA levels and enzyme activities of lipid synthesis-related genes (ACC1α and FASN). However, no significant differences were observed in mRNA levels and enzyme activities of lipolysis-related genes (HSL and CPT1). Histological observations identified two distinct hepatic phenotypes: no obvious abnormality liver and fatty liver. Overall, the proportion of fatty liver phenotype increased with the increasing feeding rate. Additionally, feeding rates significantly increased IgG, IgM, and lysozyme levels, and upregulated mRNA levels of inflammatory cytokines (<em>TNFα</em> and <em>IL1β</em>), apoptosis-related genes (<em>CASP3</em>, <em>CASP8</em>, and <em>CASP9</em>), and hepatic repair factors (<em>YAP1</em>, <em>FGF10</em>, <em>FGF13</em>, <em>FGF18</em>, <em>FGF19</em>, and <em>PCNA</em>). Nevertheless, hepatic function indices, including ALT, AST, and AKP levels, remained unchanged. In conclusion, these findings indicated that a feeding rate exceeding 3 % led to hepatic lipid metabolism disorders, characterized by the accumulation of TG and TC, thus promoting the development of fatty liver, inflammation response, and cellular apoptosis in largemouth bronze gudgeon. Moreover, the largemouth bronze gudgeon may exhibit self-repair capability to mitigate liver damage induced by lipid metabolism disorders. Consequently, we infer that largemouth bronze gudgeon possesses a robust capability for lipid accumulation and utilization.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"597 ","pages":"Article 741919"},"PeriodicalIF":3.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic profiling of the Macrobrachium rosenbergii nodavirus infection: A study of early to late-stage infection in vitro","authors":"Ken Fong Chen ,&nbsp;Wen Siang Tan ,&nbsp;Lin Kooi Ong ,&nbsp;Syafiq Asnawi Zainal Abidin ,&nbsp;Iekhsan Othman ,&nbsp;Beng Ti Tey ,&nbsp;Ronald Fook Seng Lee","doi":"10.1016/j.aquaculture.2024.741915","DOIUrl":"10.1016/j.aquaculture.2024.741915","url":null,"abstract":"<div><div>The <em>Macrobrachium rosenbergii</em> nodavirus (<em>Mr</em>NV), belonging to the <em>Nodaviridae</em> family, is responsible for a deadly infection in freshwater prawns, especially impacting the post-larvae of <em>Macrobrachium rosenbergii</em> with a mortality rate reaching 100 %. Recent research has shed light on the typical process of <em>Mr</em>NV trafficking within the host, illustrating how the virus navigates through cells as the infection advances and the subsequent cellular alterations. Yet, the specific cellular pathways disrupted by <em>Mr</em>NV, leading to these alterations, are underexplored. Furthermore, the precise effects of the <em>Mr</em>NV capsid protein, known for its strong immune response, on the host cells are not well understood. This study seeks to clarify these impacts by analysing and comparing the protein expression profiles in healthy, <em>Mr</em>NV virus-like particle (VLP) invaded, and <em>Mr</em>NV-infected Sf9 cells over a 24-h period using a mass spectrometry based proteomics approach. Our findings show that the protein expression in <em>Mr</em>NV VLP-invaded and <em>Mr</em>NV-infected Sf9 cells during the mid-infection stages is similar, involving key signalling pathways like the eukaryotic translation system, cell cycle, actin cytoskeleton regulation, and the mTOR pathway. However, changes in protein expression for key proteins such as 40S and 60S ribosomal subunits, 14–3-3 protein epsilon, and tubulin beta chain persisted only within the <em>Mr</em>NV-infection group whilst the protein expression in the VLP-invasion group reverted to baseline levels over time, underscoring the transient nature of VLP effects due to their inability to replicate. Additionally, a reduction in peroxiredoxin levels was observed in the later stages of <em>Mr</em>NV infection, indicating a potential viral strategy to trigger apoptosis and release virions. Our results suggest that <em>Mr</em>NV increases expression of 40S ribosome activity to boost viral protein synthesis while suppressing 60S ribosome expression, which impedes the synthesis of host proteins. <em>Mr</em>NV also appears to extend the lifespan of host cells by interfering with their cell cycle and blocking apoptotic pathways, thus facilitating viral replication. This research enhances our comprehension of <em>Mr</em>NV's infectious mechanism, delineates the pathways exploited by the virus, and identifies crucial molecular targets for potential therapeutic intervention.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"597 ","pages":"Article 741915"},"PeriodicalIF":3.9,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}