Aquaculture最新文献

{"title":"Effects of feed satiation on energy metabolism in the mussel Mytilus coruscus under ocean acidification and warming and possible hormonal regulation","authors":"Bole Wang ,&nbsp;Fahim Ullah Khan ,&nbsp;Menghong Hu ,&nbsp;Youji Wang","doi":"10.1016/j.aquaculture.2025.742440","DOIUrl":"10.1016/j.aquaculture.2025.742440","url":null,"abstract":"<div><div>Over the past several decades, it is thought that human activities have been a significant factor in bringing about increased atmospheric carbon dioxide concentrations, thereby contributing to elevated ocean acidification and warming. Due to their sessile nature, marine bivalves are considered to be particularly vulnerable to such alterations of the marine environment. Much remains unknown concerning the underlying strategies by which marine bivalves modify energy demand under such conditions. In addition, whether feed availability has an effect and plays a role in this respect is unclear. In the present study, we attempted to address these questions by investigating metabolism and energy pathways in the mussel <em>Mytilus coruscus</em>. Warming, acidification, and food shortage have significantly impacted metabolism, energy pathways, circadian rhythms, hormone activity, and gene expression related to metabolism and circadian rhythms in mussels, leading to energy limitations. Thermal tolerance data indicate that <em>M. coruscus</em> has substantial adaptive capacity to environmental stressors. Bivalves can regulate energy production pathways by modulating serotonin and triiodothyronine hormones, and adequate food availability enhances this regulation. Our findings suggest that ocean warming and acidification synergistically alter energy metabolism in mussels, causing energy limitations, and that food availability is crucial for maintaining mussel condition.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742440"},"PeriodicalIF":3.9,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new type of triploid fish derived from female Carassius auratus red var. × male Gobiocypris rarus","authors":"Fanglei Liu ,&nbsp;Ziyi Huang ,&nbsp;Xuanyi Zhang,&nbsp;Bei Li,&nbsp;Lujiao Duan,&nbsp;Jianming Yu,&nbsp;Qizhi Liu,&nbsp;Siyang Huang,&nbsp;Hongwen Liu,&nbsp;Qiuli Liang,&nbsp;Qingfeng Liu,&nbsp;Shaojun Liu","doi":"10.1016/j.aquaculture.2025.742442","DOIUrl":"10.1016/j.aquaculture.2025.742442","url":null,"abstract":"<div><div>Distant hybridization can combine the genomes of the parental species, leading to changes in both genotypes and phenotypes in the hybrids, often accompanied by the appearance of polyploid progeny. In this study, we obtained an allotriploid hybrid (RR, 3n = 125) through inter-subfamily hybridization between female red crucian carp (<em>Carassius auratus</em> red var., RCC, 2n = 100) and male rare gudgeon (<em>Gobiocypris rarus</em>, RG, 2n = 50). We investigated the fertilization rate, hatching rate, morphological traits, chromosome number, DNA content, 5S rDNA, mitochondrial DNA (mtDNA), growth rate and muscle fibers in RR. The results indicated that RR is an allotriploid fish with 125 chromosomes. RR exhibited a fertilization rate of 70.30 %, a hatching rate of 52.70 %, and displayed morphological traits that were intermediate between those of its parents. The 5S rDNA of RR inherited specific bands from both parents, with recombination and mutation. Through sequence alignment and the NJ evolutionary tree analysis, the mtDNA of RR showed a higher similarity to that of RCC, with some partial base insertions observed, indicating that the mitochondria of RR follows maternal inheritance. At 12 months of age, the mean weight of RR was significantly increased by 18.43 % compared to RCC. Furthermore, the total area and mean area of muscle fiber of RR increased significantly than those of RCC, indicating muscle hypertrophy and hyperplasia. This study provided a new approach to create allotriploid fish.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742442"},"PeriodicalIF":3.9,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143696625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockout of the fih gene by CRISPR/Cas9 enhances the hypoxia tolerance in grass carp (Ctenopharyngodon idella)","authors":"Huimei Zhang ,&nbsp;Ziquan Yang ,&nbsp;Jianjun Fu ,&nbsp;Ziming Yuan ,&nbsp;Jie Chen ,&nbsp;Rengao Shen ,&nbsp;Guodong Zheng ,&nbsp;Shuming Zou ,&nbsp;Zaijie Dong","doi":"10.1016/j.aquaculture.2025.742452","DOIUrl":"10.1016/j.aquaculture.2025.742452","url":null,"abstract":"<div><div>Grass carp (<em>Ctenopharyngodon idella</em>), a key freshwater fish species in China, is threatened by hypoxia in aquaculture due to high stocking densities and climate change. Factor inhibiting hypoxia-inducible factor (FIH), an asparagine hydroxylase that represses the expression of hypoxia-inducible factor (HIF), inhibits the transcription of hypoxia-responsive genes by blocking interactions between HIF and the coactivators CBP and p300. In this study, we used CRISPR-Cas9 genome editing to generate <em>fih</em>-deficient heterozygotes of grass carp. Our study demonstrates that individuals with a deletion of the <em>fih</em> gene exhibit significantly enhanced hypoxia tolerance compared to wild-type grass carp, as evidenced by the critical oxygen tension at which equilibrium is lost (LOE_crit). Furthermore, although the hypoxia tolerance of grass carp has increased, <em>fih</em> gene knockout did not have a significant impact on their growth and development. We performed real-time quantitative PCR and TUNEL staining to assess hypoxic responses in <em>fih</em>-deficient grass carp, and accordingly found that compared with the wild grass carp, certain hypoxia-responsive genes, including <em>hif1α</em>, <em>phd3</em>, and g<em>lut1</em>, were upregulated in <em>fih</em>-deficient individuals. Additionally, we established that, under hypoxic conditions, levels of apoptosis in the gills and brains of <em>fih</em>-deficient fish were significantly lower than those in their wild counterparts. These findings indicate that <em>fih</em> plays an essential role in hypoxia tolerance of grass carp and providing candidate genes for the genetic breeding of hypoxia-tolerant varieties.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742452"},"PeriodicalIF":3.9,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A TaqMan probe-based RT-qPCR for the detection of brine shrimp reovirus 1","authors":"Xuan Li ,&nbsp;Guohao Wang ,&nbsp;Haoyu Lou ,&nbsp;Chengyan Zhou ,&nbsp;Xiangmin Meng ,&nbsp;Xinping Wang ,&nbsp;Xuan Dong","doi":"10.1016/j.aquaculture.2025.742444","DOIUrl":"10.1016/j.aquaculture.2025.742444","url":null,"abstract":"<div><div>Brine shrimp (<em>Artemia</em>) are widely utilized as a vital live feed in the aquaculture industry. Recently, we found a novel reovirus - brine shrimp reovirus 1 (BSR1) in brine shrimp. Considering the potential impact of reovirus on the industry, it is necessary to develop a highly sensitive and specific detection method in advance for identifying BSR1. In this study, we established a real-time quantitative reverse transcription PCR (RT-qPCR) method based on TaqMan probe. The detection limit of this method is as low as 4.4 × 10¹ copies/reaction. The standard curve between 4.4 × 10¹ to 4.4 × 10⁹ copies/reaction showed a high correlation coefficient (R² = 0.997). This newly developed method was used to screen for BSR1 in brine shrimp cyst samples from Africa, Asia, Europe, Oceania, South America and North America, and the results showed that BSR1 was detected in samples from Asia and North America. The brine shrimp cyst samples tested covered seven different species, with BSR1-positive samples found in <em>A. franciscana</em>, <em>A. urmiana</em>, <em>A. sinica</em>, and parthenogenetic <em>Artemia</em> lineage. The diagnostic sensitivity (DSe) and diagnostic specificity (DSp) values of the newly developed RT-qPCR method, when compared to the high-throughput sequencing, were determined to be 100 % and 88 %. This newly developed RT-qPCR method can serve as a crucial tool for detecting BSR1, facilitating early warning and swift response to viruses carried by brine shrimp, thereby effectively mitigating the risk of pathogen transmission to cultured animals.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742444"},"PeriodicalIF":3.9,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptomic and metabolomic analysis reveals molecular mechanisms of infectious myonecrosis virus (IMNV) pathogenesis in Litopenaeus vannamei muscle tissue","authors":"Yan Rong ,&nbsp;Yi Qiao ,&nbsp;Xiaohui Cao ,&nbsp;Ge Jiang ,&nbsp;Jie Cheng ,&nbsp;Huiwen Jiang ,&nbsp;Hui Shen","doi":"10.1016/j.aquaculture.2025.742443","DOIUrl":"10.1016/j.aquaculture.2025.742443","url":null,"abstract":"<div><div>The Infectious myonecrosis virus (IMNV) poses a significant threat to <em>Litopenaeus vannamei</em>, and there is limited research on the transcriptomic and metabolomic changes in shrimp muscle tissue post-infection. This study aims to integrate transcriptomic and metabolomic data to elucidate the gene expression and metabolic alterations in shrimp muscle tissue induced by IMNV. We present a molecular model of IMNV invasion and the mechanisms leading to muscle cell necrosis. Sequencing analyses at 30, 60, and 90 days post-infection identified 695, 2411, and 401 differentially expressed genes (DEGs) and 118, 131, and 68 significantly different metabolites (SDMs), respectively. In the early infection stage (IE, 30 days), we observed alterations in gene expression related to glucose and lipid metabolism, along with upregulation of antioxidant-related genes. The intermediate stage (IM, 60 days) exhibited significant reprogramming of metabolic pathways and signal transduction. In the persistent infection stage (IP, 90 days), we noted significant changes in energy metabolism and cell repair-related gene expression, with upregulation of genes involved in glycolysis and fatty acid biosynthesis to maintain energy supply.</div><div>Our proposed model for IMNV invasion encompasses viral receptor recognition, endosomal escape, replication, and maintenance of the host cell environment. IMNV binds to host cells via the laminin receptor (Lamr), with the SMPD1 gene facilitating endosomal escape through ceramide production. The PI3K-Akt-mTOR pathway provides energy for viral replication, while the JAK-STAT pathway may be hijacked by IMNV. The virus reprograms metabolism by regulating pathways and metabolites such as <span>d</span>-glucose-6-phosphate, D-aspartic acid, and L-glutamic acid, while enhancing autophagy and regulating sphingolipid metabolism and antioxidant mechanisms. During viral particle assembly and release, SMPD1 promotes necroptosis and ceramide production. Additionally, IMNV modulates cytoskeletal remodeling and adhesion by regulating the Act57B, Act5C, and Act88F genes. IMNV leads to host muscle cell necrosis by suppressing the immune response, inducing cytoskeletal remodeling, oxidative stress, and activating cell death pathways such as apoptosis and ferroptosis. These processes disrupt the structure and function of muscle cells, leading to extensive necrosis. This study provides insights into the pathogenic mechanisms of IMNV and establishes a foundation for the development of anti-IMNV strategies.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742443"},"PeriodicalIF":3.9,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteria in land-based larval rearing systems: Sources of bacteria and their quantitative and qualitative influence on the microbiota of rearing water and larvae","authors":"Sujan Khadka ,&nbsp;Olav Vadstein ,&nbsp;Ingrid Bakke ,&nbsp;Yngvar Olsen ,&nbsp;Kari J.K. Attramadal","doi":"10.1016/j.aquaculture.2025.742445","DOIUrl":"10.1016/j.aquaculture.2025.742445","url":null,"abstract":"&lt;div&gt;&lt;div&gt;In aquaculture, mortality and poor performance during early life stages remain major bottlenecks. It is well-documented that detrimental larvae-microbe interactions significantly cause these problems. To develop microbial management strategies, it is crucial to understand where microbes in the rearing tank come from, what determines their community composition, and how microbes from live feed and water affect the gut microbiota of larvae. This study aimed to combine previously published and new data to address these questions for the larval rearing of Atlantic cod (&lt;em&gt;Gadus morhua&lt;/em&gt; L.). Three rearing system designs were analyzed: a flow-through system (FTS), a FTS with a biofilter for microbial maturation (MMS), and a recirculating aquaculture system (RAS). Sources of bacteria in the rearing tank included external sources of bacteria (i.e., incoming water, microalgae, and live feed) and growth of bacteria in the rearing tanks. Our results supported that careful preparation and manipulation of the initial bacterial community can improve rearing conditions, particularly in FTS and MMS, by impacting the initial colonization. A lower bacterial carrying capacity in the inflowing water compared to the tank water was a challenge in FTS and MMS, as the increased access to organic matter led to substantial internal growth of bacteria in the rearing tank. Bacterial production within the rearing tank relative to incoming bacteria from water was measured to be 89 % and 94 % in FTS and MMS, respectively, compared to 49 % in RAS. Quantitatively, the live feed was the dominant source of bacteria to the larval gut (&gt;95 %). The uptake of bacteria by larvae from the rearing water was higher in RAS (3.28 %) than in FTS (0.86 %) and MMS (0.75 %). Although the live feed microbiota dominated the number of microbes entering the gut in this study, previous research has shown that the microbial composition of the larval gut generally has higher similarity to the rearing water than to the bacteria associated with the live feed. This can be explained by the selection pressure created in/by the host. Our results also showed that biofilters significantly affected bacterial composition and density by enhancing microbial richness and evenness in the water. Depending on the organic matter load, biofilters release substantial numbers of bacteria into the water, potentially structuring the microbial environment for larvae. The microbial influence of biofilters has been shown in previous studies to enhance larval survival, i.e. higher survival in RAS and MMS compared to FTS. However, more research is necessary to explain the specific role that bacterial communities play in promoting larval health and survival. This study is the first attempt to make a bacterial budget for various larval-rearing systems. It shows that rearing technology has a tremendous impact on the microbial quality that the reared larvae are exposed to, and this new knowledge can be used to dev","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742445"},"PeriodicalIF":3.9,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetics of honokiol in Trachinotus ovatus following administration of honokiol monomer and Magnolia officinalis extracts","authors":"Weiliang Guo ,&nbsp;Danyi Wei ,&nbsp;Zichen Zhao ,&nbsp;Fengxiao Hu ,&nbsp;Dahao Wang ,&nbsp;Wei Tang ,&nbsp;Yan Wang ,&nbsp;Hengwei Deng ,&nbsp;Jianlong Li ,&nbsp;Dongdong Zhang ,&nbsp;Zhihong Zhong ,&nbsp;Yongcan Zhou","doi":"10.1016/j.aquaculture.2025.742438","DOIUrl":"10.1016/j.aquaculture.2025.742438","url":null,"abstract":"<div><div>Honokiol, an active ingredient in <em>Magnolia officinalis</em>, has a good efficacy in controlling <em>Cryptocaryon irritans</em> infection, which can parasitize in the gill and epidermis of fish and cause mass mortality to marine fish cultures. However, the lack of pharmacokinetic information on honokiol in fish has limited its use. Here, a high-performance liquid chromatography (HPLC) method for detecting honokiol in fish tissues was developed and used to compare the pharmacokinetics of honokiol following oral administration of pure honokiol (HTS group) and <em>M. officinalis</em> extracts (METS group) in <em>Trachinotus ovatus</em> at a dose of 12 mg/kg at 27 °C. Honokiol was rapidly absorbed by fish and showed a bimodal phenomenon in the plasma. The maximum concentration (<em>C</em>_max) in plasma was at 0.5 h in both the HTS (38.92 μg/mL) and METS groups (14.58 μg/mL). The <em>C</em>_max of honokiol in the gill and epidermis in the HTS group (239.69 μg/g and 46.39 μg/g, respectively) were higher than in the METS group (9.44 μg/g and 14.30 μg/g, respectively). However, the elimination half-life (<em>t</em>_1/2) of honokiol in the plasma, gill, and epidermis in the HTS group (0.93 h, 1.26 h, and 1.32 h, respectively) were shorter than the METS group (1.12 h, 3.11 h, and 5.14 h, respectively). In both treatment group, honokiol was completely eliminated from all tissues at 12 h post oral administration. Compared with the HTS group, the bioavailability (<em>F</em>_r) of honokiol in the METS group increased in the plasma (105.26 %), liver (489.58 %), and muscle (796.91 %), but decreased in the gill (10.94 %), kidney (74.22 %), and epidermis (93.69 %). This study revealed the pharmacokinetic profile of honokiol and indicates that improved efficacy for controlling <em>C. irritans</em> infestation could be obtained by oral administration of pure honokiol rather than with <em>M. officinalis</em> extracts.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742438"},"PeriodicalIF":3.9,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal variation in two culture systems and genome-wide association analysis of taurine content in the foot muscles of the Pacific abalone (Haliotis discus hannai)","authors":"Ziheng Yin ,&nbsp;Junyu Liu ,&nbsp;Wenchao Yu ,&nbsp;Yawei Shen ,&nbsp;Yang Gan ,&nbsp;Yexin Chen ,&nbsp;Jinwei Ke ,&nbsp;Xuan Luo ,&nbsp;Caihuan Ke ,&nbsp;Weiwei You","doi":"10.1016/j.aquaculture.2025.742431","DOIUrl":"10.1016/j.aquaculture.2025.742431","url":null,"abstract":"<div><div>Abalone is one of the most economically valuable shellfish in the world's aquaculture industry, and as production has increased, it has led to a diversification of market quality needs. Taurine, an essential amino acid component, has yet to be well investigated for its seasonal variation and genetically associated genes or loci in Pacific abalone (<em>Haliotis discus hannai</em>). In this study, we investigated the variation of taurine content in different seasons under two typical culturing systems in southern China. In addition, we conducted a genome-wide association study (GWAS) with 274 abalone array sequencing and phenotypic data of taurine content. The results revealed that the taurine content showed a trend of significantly decreasing and then increasing with seasonal changes. Individuals farmed in land-based industrial culture system had a greater taurine content than those farmed in sea-based floating raft culture system, with notable differences observed between May and September. A total of 69,530 high-quality SNPs and 274 individuals were identified by quality control. And 32 significantly related SNP loci of taurine content were identified by GWAS, with chromosome 9 hosting the lowest <em>P</em> value. A total of 33 candidate genes, including <em>SLC6A7</em>, <em>EGF1</em>, <em>B3gnt6</em>, and <em>Ptpra</em>, were annotated. These genes may be connected to amino acid synthesis, transmembrane transport, energy production, protein phosphorylation, and other processes. Among them, genes belonging to the <em>SLC6A</em> family may be the key gene taurine content. This study helped us better understand the genetics of the taurine content of Pacific abalone.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742431"},"PeriodicalIF":3.9,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The virulence role of the fliQ gene of Vibrio harveyi and its effect on the immune response of the large yellow croaker (Larimichthys crocea)","authors":"Zhihang Ouyang ,&nbsp;Xiangrong Tian ,&nbsp;Zhenyuan Zhao ,&nbsp;Jing Diao ,&nbsp;Le Li ,&nbsp;Liang Miao ,&nbsp;Yang Du ,&nbsp;Jiong Chen","doi":"10.1016/j.aquaculture.2025.742439","DOIUrl":"10.1016/j.aquaculture.2025.742439","url":null,"abstract":"<div><div>In this study, we focused on <em>Vibrio harveyi</em>, a prevalent pathogen in aquaculture. Previous research has shown that its flagella and the flagellar synthesis protein FliQ play a vital role in adhesion, infection, and virulence. We constructed a <em>fliQ</em> gene deletion mutant strain (<em>ΔfliQ</em>) from the wild-type strain LcV6 (Wt-LcV6) to investigate the function of <em>fliQ</em> in <em>V. harveyi</em> and evaluated its biological characteristics. Through transcriptome analysis, we compared the gene expression levels between Wt-LcV6 and <em>ΔfliQ</em>. Also, we intraperitoneally injected <em>ΔfliQ</em> into large yellow croakers for immunization to assess its protective effect. The results indicated that the growth and biofilm synthesis abilities of <em>ΔfliQ</em> were similar to those of the wild type. However, the motility of <em>ΔfliQ</em> notably decreased. Transcriptome analysis demonstrated that the deletion of the <em>fliQ</em> gene caused a significant reduction in the mRNA levels of <em>gltD</em>, <em>malE</em>, <em>malQ</em>, <em>lamB</em>, and <em>pulA</em>, primarily affecting pathways related to sulfur metabolism, selenium metabolism, monoacyl lactam biosynthesis, and several amino acid and carbohydrate metabolisms. Moreover, after immunization with <em>ΔfliQ</em>, the large yellow croakers had a relative protection rate of 43.3 % against <em>V. harveyi</em>. <em>ΔfliQ</em> effectively induced an increase in the specific IgM antibody level, significantly enhanced the activities of CAT, AKP, and SOD, and promoted the expression of immune-related genes (<em>IgM</em>, <em>IgD</em>, <em>TGF</em>, <em>IL-1</em>, <em>TNF</em>, <em>MHC II</em>, <em>CD4</em>, and <em>CD8</em>) in the liver, spleen, and kidney of the fish. In conclusion, the <em>fliQ</em> gene is a crucial virulence factor of <em>V. harveyi</em>. The <em>ΔfliQ</em> can effectively boost the immunity of immunized fish, making it a promising candidate for a vaccine against <em>V. harveyi</em>. This research provides a new direction for developing attenuated vaccines against this pathogen, which has practical significance for aquaculture disease prevention and control.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"603 ","pages":"Article 742439"},"PeriodicalIF":3.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revisiting occupational health and safety in Norwegian fish farming – Results from an employee survey","authors":"Trine Thorvaldsen ,&nbsp;Trond Kongsvik ,&nbsp;Ingunn Marie Holmen ,&nbsp;Kristine Størkersen ,&nbsp;Siri Holen","doi":"10.1016/j.aquaculture.2025.742441","DOIUrl":"10.1016/j.aquaculture.2025.742441","url":null,"abstract":"<div><div>While researchers have called for a global commitment to occupational health and safety (OHS) in aquaculture, this topic is seldom a part of the sustainability discourse in salmon fish farming.</div><div>To provide updated knowledge regarding OHS in Norwegian salmon farming and identify possible determinants for risk exposure a digital survey was conducted in 2023. It was answered by 1283 employees working in fish-farming companies, for independent shipowners/service vessel companies, or for supplier companies.</div><div>The results showed that most employees consider their health to be good and are satisfied at work. Still, many report worries (62 %) and work-related absences (17 %) due to strain/musculoskeletal injuries or complaints and acute injuries due to accidents. While most agree that their company takes safety seriously (81 %), several respondents report that considerations regarding production are prioritized at the expense of safety (29 %) and that inadequate maintenance has reduced safety (28 %). Moreover, 31 % agree that it is uncomfortable to point out a lack of compliance with safety rules and procedures, and 36 % reported that they did not participate in developing new procedures. Logistic regression analysis shows that risk determinants include being a younger worker, working sea-based and experiencing high efficiency demands. These groups had a higher probability of near-accident exposure.</div><div>Several suggestions for improvement are presented. More can be done to involve employees in risk assessments and procedures. Technological measures can help reduce or eliminate ergonomic and other hazards, and companies may benefit from open dialogue regarding work pressures and feelings of discomfort when pointing out breaches of safety rules. Dedicated coordinators of large operations can ensure the well-being and safety of workers, and increased cooperation across organizations is also suggested. Those who desire to create a sustainable industry must focus on improving OHS. Employee surveys are valuable for systematic OHS work, identifying vulnerable groups, as well as increasing awareness at the company and industry levels.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"603 ","pages":"Article 742441"},"PeriodicalIF":3.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}