Aquaculture最新文献_第10页

Validation of QTL associated with resistance to Lernanthropus kroyeri in European seabass 欧洲鲈鱼对克氏弧菌抗性相关QTL的验证

IF 3.9 1区农林科学

Aquaculture Pub Date : 2025-05-26 DOI: 10.1016/j.aquaculture.2025.742763

Stavroula Oikonomou , Domniki Manousi , Arkadios Dimitroglou , Zoi Kazlari , Dimitrios Loukovitis , Kantham Papanna , Konstantinos Tzokas , Nikos Katribouzas , Leonidas Papaharisis , Costas S. Tsigenopoulos , Dimitrios Chatziplis

{"title":"Validation of QTL associated with resistance to Lernanthropus kroyeri in European seabass","authors":"Stavroula Oikonomou , Domniki Manousi , Arkadios Dimitroglou , Zoi Kazlari , Dimitrios Loukovitis , Kantham Papanna , Konstantinos Tzokas , Nikos Katribouzas , Leonidas Papaharisis , Costas S. Tsigenopoulos , Dimitrios Chatziplis","doi":"10.1016/j.aquaculture.2025.742763","DOIUrl":"10.1016/j.aquaculture.2025.742763","url":null,"abstract":"<div><div><em>Lernanthropus kroyeri</em> constitutes the most commonly encountered parasitic copepod for European seabass (<em>Dicentrarchus labrax</em>). Infection of gills with copepods directly impacts fish survival and growth performance with further economic implications for the aquaculture industry. Earlier studies identified moderate heritability of parasite count and two suggestive candidate QTL that explained 2 % of the total phenotypic variance each. The present study focuses on the two previously identified QTL loci to validate any QTL effect on <em>L. kroyeri</em> parasite resistance. In order to facilitate the validation of healthy and infected fish, individuals were selected based on their resistance to parasites, specifically the presence or absence of L. <em>kroyeri</em> in fish reared in a standard open sea cage. A subset of samples was genotyped using the 30 k MedFISH SNP array and the putative QTL effects were tested for parasite resistance by fitting an animal model in ASREML-R while the AIC criterion was used to assess model fit. The <em>p</em>-values for the two SNP with highest association to parasite resistance AX-373127007 and AX-373218583 were 0.041 (<0.05) and 0.085 (<0.1), respectively. Despite the moderate significance of the second SNP, the lowest AIC score was detected after using both SNPs as fixed effects in the animal model. The findings of the present study, using a different population, validate the important role of the detected QTL loci on parasite resistance, highlighting a possible application of a low-cost Marker Assisted Selection (MAS) breeding program in farmed European seabass populations.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"608 ","pages":"Article 742763"},"PeriodicalIF":3.9,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Removal of residual antibiotics from aquaculture effluents by denitrifying woodchip bioreactors 反硝化木屑生物反应器去除水产养殖废水中的残留抗生素

IF 3.9 1区农林科学

Aquaculture Pub Date : 2025-05-26 DOI: 10.1016/j.aquaculture.2025.742761

Mathis von Ahnen , Kai Tang , Ida van de Ven , Adam C. Hambly , Henrik Rasmus Andersen , Niels Lorenzen , Per Bovbjerg Pedersen

{"title":"Removal of residual antibiotics from aquaculture effluents by denitrifying woodchip bioreactors","authors":"Mathis von Ahnen , Kai Tang , Ida van de Ven , Adam C. Hambly , Henrik Rasmus Andersen , Niels Lorenzen , Per Bovbjerg Pedersen","doi":"10.1016/j.aquaculture.2025.742761","DOIUrl":"10.1016/j.aquaculture.2025.742761","url":null,"abstract":"<div><div>The aquaculture industry lacks effective and economical treatment processes to reduce the risk of antibiotic residues in its effluents. In this study, the removal performance of end-of-pipe denitrifying woodchip bioreactors on four commonly used antibiotics was determined. Laboratory batch experiments were performed to investigate the abiotic adsorption capacity of woodchips, while laboratory continuous-flow tests and monitoring of full-scale woodchip bioreactors in the field were performed to investigate antibiotic removal by woodchips during treatment of aquaculture effluents. Adsorption of oxolinic acid was most effective, with the highest measured adsorption capacity of 21 μg g<sup>−1</sup> achieved at an equilibrium concentration of 15 μg L<sup>−1</sup> in the water. Binding of trimethoprim and sulfadiazine was well described by Langmuir isotherms with maximum adsorption capacities of 106 μg g<sup>−1</sup> and 27 μg g<sup>−1</sup> respectively. In contrast, florfenicol was poorly adsorbed by woodchips. The results from continuous-flow experiments demonstrated the removal efficiencies for trimethoprim, sulfadiazine, and oxolinic acid to be 89 %, 64 %, and 63 %, respectively, while florfenicol was not effectively removed.</div><div>During the monitoring of a full-scale bioreactor, removals of 77 % and 93 % were observed for trimethoprim and oxolinic acid, respectively. In contrast, the concentration of sulfadiazine increased by 141 % in the bioreactor due to hydrolysis/deconjugation of its metabolite acetyl sulfadiazine. Overall, this study demonstrates that end-of-pipe denitrifying woodchip bioreactors can act as safety barriers to the potential discharge of certain antibiotics from recirculated freshwater aquaculture systems, providing substantial reductions, particularly for oxolinic acid and trimethoprim, which are commonly used for disease treatment in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"608 ","pages":"Article 742761"},"PeriodicalIF":3.9,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life cycle assessment of Tilapia production via conventional aquaculture and aquaponic systems 通过传统水产养殖和水培系统生产罗非鱼的生命周期评估

IF 3.9 1区农林科学

Aquaculture Pub Date : 2025-05-26 DOI: 10.1016/j.aquaculture.2025.742760

Sumonrat Chairat , Sujata Regmi , Hsiang-Wei Cheng , Phatchaploy Vongmahadlek , Shabbir H. Gheewala

{"title":"Life cycle assessment of Tilapia production via conventional aquaculture and aquaponic systems","authors":"Sumonrat Chairat , Sujata Regmi , Hsiang-Wei Cheng , Phatchaploy Vongmahadlek , Shabbir H. Gheewala","doi":"10.1016/j.aquaculture.2025.742760","DOIUrl":"10.1016/j.aquaculture.2025.742760","url":null,"abstract":"<div><div>The rapid growth of aquaculture towards global food security presents environmental challenges necessitating the adoption of sustainable practices and technologies. Aquaponics is potentially one of the Nature-based Solutions (NbS), relying on the symbiosis between fish, bacteria, and plants to create a sustainable system that enhances food and water security while purportedly reducing environmental impacts. The study assesses and compares the environmental performance of aquaponics producing tilapia and lettuce with corresponding conventional production using life cycle assessment (LCA). The results revealed that aquaponics tilapia generally performs worse than conventional production in all environmental impact categories, except for eutrophication, terrestrial ecotoxicity, and water consumption. Partially replacing fish meal with plant-based protein (e.g., soybean meal) in tilapia feed can generally reduce the environmental impact of tilapia production in both conventional systems and aquaponics. Using solar energy can improve the environmental performance of tilapia production in aquaponics. Replacing fishmeal with soybean meal in fish feed and using solar energy in fish farming can make the performance of aquaponics tilapia better than conventional production in most categories, except for terrestrial ecotoxicity and human toxicity. From the perspective of lettuce production, even after improvements, aquaponics lettuce still performs worse than conventional production across all impact categories, except for terrestrial acidification and marine eutrophication. This highlights that NbS, such as aquaponics, may not always guarantee better outcomes than conventional production systems. The trade-off between environmental benefits in certain areas and the higher energy demands highlights the challenge of adopting aquaponics as a sustainable alternative to conventional farming.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"608 ","pages":"Article 742760"},"PeriodicalIF":3.9,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ophryotrocha craigsmithi (Wiklund, Glower & Dahlgren, 2009) has a high capacity to modify lipids from aquaculture waste and synthesize long-chain polyunsaturated fatty acids Ophryotrocha craigsmithi （Wiklund, Glower & Dahlgren, 2009）具有从水产养殖废弃物中改性脂类和合成长链多不饱和脂肪酸的高能力

IF 3.9 1区农林科学

Aquaculture Pub Date : 2025-05-25 DOI: 10.1016/j.aquaculture.2025.742746

Signe Gunborg Bentzrød Svensson , Sonnich Meier , Svein A. Mjøs , Tore Strohmeier , Henrice M. Jansen

{"title":"Ophryotrocha craigsmithi (Wiklund, Glower & Dahlgren, 2009) has a high capacity to modify lipids from aquaculture waste and synthesize long-chain polyunsaturated fatty acids","authors":"Signe Gunborg Bentzrød Svensson , Sonnich Meier , Svein A. Mjøs , Tore Strohmeier , Henrice M. Jansen","doi":"10.1016/j.aquaculture.2025.742746","DOIUrl":"10.1016/j.aquaculture.2025.742746","url":null,"abstract":"<div><div>In Norway, <em>Ophryotrocha craigsmithi</em> is being evaluated as an extractive species in integrated multitrophic aquaculture systems (IMTA) and has been suggested as a suitable novel feed ingredient in aquatic feeds due to its high content of polyunsaturated fatty acids (PUFA). It has been hypothesized that <em>O. craigsmithi</em> is capable of trophic up-grading, and that bacterial mats play a crucial role in this species diet, enhancing growth rates and defining FA profiles. We studied the FA uptake and metabolism in <em>O. craigsmithi</em> reared on diets consisting of commercial fish pellets, bacteria incubated fish pellets or sludge collected <em>in situ</em>. We aimed to confirm whether <em>O. craigsmithi</em> performs trophic up-grading, and to elucidate the role of white bacterial mats for this species. FA profiles of diets and polychaetes were determined using GC-FID analysis, and lipid classes were separated by HPLC. Results indicate that <em>O. craigsmithi</em> performs trophic-upgrading and particularly produces high amounts of 20:4n-6 and 20:5n-3. We suggest that <em>O. craigsmithi</em> applies the Δ12 (methyl-end) enzyme to produce 18:2n-6 <em>de novo</em> and further use this precursor to produce 20:4n-6 before the Δ17 (methyl-end) enzyme is used for the final desaturation to 20:5n-3. Analysis of the phospholipids of polychaetes showed that FA profiles remained stable independent of diet, illustrating a high priority for cell membrane functionality. Bacterial FAs were mainly used as energy, although c18:1n-7 was selectively incorporated into cell membranes in polychaetes. Our results highlight this species' value in converting farm waste to high quality marine long-chain PUFA in IMTA systems.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"608 ","pages":"Article 742746"},"PeriodicalIF":3.9,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An SNP for the splice donor variant of i-type lysozyme 2 (lyz-i2) is associated with the resistance of Vibrio harveyi in Pacific white shrimp i型溶菌酶2剪接供体变异（lyz-i2）的SNP与太平洋白虾对哈维弧菌的抗性有关

IF 3.9 1区农林科学

Aquaculture Pub Date : 2025-05-25 DOI: 10.1016/j.aquaculture.2025.742730

Xin Zhang , Peng Luo , Chunhua Ren , Pengying Li , Yehui Tan , Hao Yang , Shuyang Wen , Bo Ma , Jiayue Yin , Yanhong Wang , Xiao Jiang , Jingxuan Liang , Guiling Tan , Chaoqun Hu , Ting Chen

{"title":"An SNP for the splice donor variant of i-type lysozyme 2 (lyz-i2) is associated with the resistance of Vibrio harveyi in Pacific white shrimp","authors":"Xin Zhang , Peng Luo , Chunhua Ren , Pengying Li , Yehui Tan , Hao Yang , Shuyang Wen , Bo Ma , Jiayue Yin , Yanhong Wang , Xiao Jiang , Jingxuan Liang , Guiling Tan , Chaoqun Hu , Ting Chen","doi":"10.1016/j.aquaculture.2025.742730","DOIUrl":"10.1016/j.aquaculture.2025.742730","url":null,"abstract":"<div><div><em>Litopenaeus vannamei</em> is a crucial crustacean for global aquaculture due to its high economic value. However, vibriosis poses a significant threat to shrimp farming. Molecular breeding techniques such as marker-assisted selection and genomic selection offer efficient approaches to enhance disease resistance. The i-type lysozyme 2 (lyz-i2) plays a pivotal role in innate immunity in <em>Penaeid</em> shrimp. This study identified a splice donor variant SNP in <em>Lvlyz-i2</em>, uncovering a novel long-form isoform. Comparative analysis of <em>Lvlyz-i2</em> isoforms across crustaceans revealed widespread alternative splicing of short and long isoforms. A G/A mutation at the intron II/exon III junction facilitated the novel long-form <em>Lvlyz-i2</em> isoform. Compared with the Lvlyz-i2L, Lvlyz-i2S exhibited stronger bacteriolytic activity against Gram-negative bacteria, <em>Vibrio harveyi</em>. Additionally, an amplification-refractory mutation system for detecting SNP 1271 G > A was developed, enabling easy identification of genotypes (625 bp for A allele, 221 bp for G allele, and 835 bp as a control fragment). Association analysis indicated that SNP 1271 G > A significantly influenced the resistance to <em>V. harveyi</em>, with the GG genotype positively affecting anti-<em>V. harveyi</em> traits in L. <em>vannamei</em> (<em>p</em>) < 0.05). In conclusion, this study firstly identified a molecular marker for selecting <em>V. harveyi</em> resistance trait in L. <em>vannamei</em> and provided a rapid, efficient detection method for the SNP locus, thereby accelerating molecular breeding efforts.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"608 ","pages":"Article 742730"},"PeriodicalIF":3.9,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the efficacy of Corbicula fluminea in controlling Ichthyophthirius multifiliis infections 河狸Corbicula fluinea防治多filitis感染的效果评价

IF 3.9 1区农林科学

Aquaculture Pub Date : 2025-05-25 DOI: 10.1016/j.aquaculture.2025.742758

Tian Qin , Yang-Yang Pan , Ke Huang , Wei-Shan Zhao , Ying-Zi Pan , Hong Zou , Wen-Xiang Li , Shan-Gong Wu , Gui-Tang Wang , Ming Li

引用次数: 0

Identification of KASP-SNP markers correlated with both growth and hypoxia tolerance traits in blunt snout bream (Megalobrama amblycephala) 钝口鲷（Megalobrama amblycephala）生长和耐缺氧性状相关KASP-SNP标记的鉴定

IF 3.9 1区农林科学

Aquaculture Pub Date : 2025-05-25 DOI: 10.1016/j.aquaculture.2025.742745

Yanluo Zhuo , Han Wang , Guanghai Dong , Huihu Wang , Guodong Zheng , Shuming Zou

{"title":"Identification of KASP-SNP markers correlated with both growth and hypoxia tolerance traits in blunt snout bream (Megalobrama amblycephala)","authors":"Yanluo Zhuo , Han Wang , Guanghai Dong , Huihu Wang , Guodong Zheng , Shuming Zou","doi":"10.1016/j.aquaculture.2025.742745","DOIUrl":"10.1016/j.aquaculture.2025.742745","url":null,"abstract":"<div><div>The blunt snout bream (<em>Megalobrama amblycephala</em>) is hypoxia-sensitive fish. Molecular selection is an accurate and fast method for cultivating good breeds of fish; however, the key genes or molecular markers related to both growth and hypoxia tolerance used for breeding are relatively few in blunt snout bream. Based on single-nucleotide polymorphism (SNP) sites from four genome resequencing databases of blunt snout bream, 230 SNPs related to hypoxia and growth were screened. Subsequently, the Kompetitive allele-specific PCR (KASP) method was used to genotype SNPs in 248 fish, and 203 KASP-SNP markers were successfully designed, with a success rate of 88.3 % and an average accuracy rate of 90.42 % for each detection site. Moreover, six specific SNPs located in six genes <em>(ho1a</em>, <em>ho1b</em>, <em>pfkfb3</em>, <em>egln2</em>, <em>fzd9b</em> and <em>igf1rb</em>) related to growth and hypoxia tolerance were identified through mixed linear model (MLM) analysis. The body weight of the fish group consisting of the above six dominant genotypes increased by 14.15 % compared to the weight value of the total population, and the loss of equilibrium (LOE<sub>crit</sub>) value decreased by 10.75 % to 0.614. The accuracy of the screened SNPs was verified using an external population of 200 fish. Consistently, the body weight of the fish group with the dominant genotype increased by 15.23 % compared to that of the total population, and the LOE<sub>crit</sub> value decreased by 10.12 % to 0.591. The results of the present study provide a theoretical basis for the molecular breeding of blunt snout bream and help cultivate new varieties with excellent growth and hypoxia tolerance.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"608 ","pages":"Article 742745"},"PeriodicalIF":3.9,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Upregulation of glutamine Fructose-6-phosphate aminotransferase 2 (GFPT2) in response to decapod iridescent virus 1 (DIV1) infection in Cherax quadricarinatus 谷氨酰胺果糖-6-磷酸转氨酶2 （GFPT2）对十足虫彩虹病毒1 （DIV1）感染的响应

IF 3.9 1区农林科学

Aquaculture Pub Date : 2025-05-25 DOI: 10.1016/j.aquaculture.2025.742759

Qin Zheng , Xiaojuan Chen , Furong Zhao , Boyuan Zheng , Wei Wang , Yongmei Zheng , Jianming Chen

{"title":"Upregulation of glutamine Fructose-6-phosphate aminotransferase 2 (GFPT2) in response to decapod iridescent virus 1 (DIV1) infection in Cherax quadricarinatus","authors":"Qin Zheng , Xiaojuan Chen , Furong Zhao , Boyuan Zheng , Wei Wang , Yongmei Zheng , Jianming Chen","doi":"10.1016/j.aquaculture.2025.742759","DOIUrl":"10.1016/j.aquaculture.2025.742759","url":null,"abstract":"<div><div>Glutamine fructose-6-phosphate aminotransferase 2 (GFPT2), the first and rate-limiting enzyme in the hexosamine biosynthetic pathway (HBP), has been implicated in the host's antiviral immune response. Nevertheless, its role in defending against Decapod iridescent virus 1 (DIV1), a pathogen severely threatening the aquaculture industry, remains largely uncharacterized. Here, we systematically investigated the characteristics of GFPT2 in <em>Cherax quadricarinatus</em> and its interplay with DIV1 infection. Employing immunohistochemistry and Western blot analysis, we demonstrated that GFPT2 is ubiquitously expressed across diverse tissues of <em>C. quadricarinatus</em>. Immunofluorescence assays further revealed its cytoplasmic localization within the hematopoietic tissue cells. Notably, our results showed a significant upregulation of GFPT2 expression during DIV1 infection. Significantly, RNA interference-mediated suppression of GFPT2 expression led to a remarkable increase in viral replication, strongly indicating that GFPT2 serves as a critical antiviral factor. These findings not only shed new light on the complex interaction between DIV1 and its hosts, but also identify GFPT2 as a potential target for developing novel strategies to combat DIV1 infection.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"608 ","pages":"Article 742759"},"PeriodicalIF":3.9,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validating a proxy to carrying capacity for finfish offshore aquaculture in the western Mediterranean 验证地中海西部有鳍鱼类近海水产养殖承载能力的代理

IF 3.9 1区农林科学

Aquaculture Pub Date : 2025-05-25 DOI: 10.1016/j.aquaculture.2025.742756

E. Carbonell-Garzon , V. Fernandez-Gonzalez , F. Romero , P. Sanchez-Jerez , Ll. Belda , M.M. Agraso , K. Toledo-Guedes

{"title":"Validating a proxy to carrying capacity for finfish offshore aquaculture in the western Mediterranean","authors":"E. Carbonell-Garzon , V. Fernandez-Gonzalez , F. Romero , P. Sanchez-Jerez , Ll. Belda , M.M. Agraso , K. Toledo-Guedes","doi":"10.1016/j.aquaculture.2025.742756","DOIUrl":"10.1016/j.aquaculture.2025.742756","url":null,"abstract":"<div><div>After developing a proxy for carrying capacity (CC) for marine finfish aquaculture integrating physical, ecological, social, and economic factors (Romero et al.,2023), this study presents an <em>in situ</em> validation of the proxy. To achieve this, we applied the harmonized Environmental Monitoring Program (EMP) developed by Aguado-Giménez et al. (2012) for finfish aquaculture farms in the Mediterranean Sea. We hypothesised that if the proxy indicated CC had been exceeded, some variables would surpass acceptable thresholds. Our findings confirmed that two of the three facilities exceeded thresholds for one or more indicator variables. However, this study focused on environmental CC, lacking defined thresholds for socio-economic indicators. This gap underscores the necessity for developing social and economic indicators to identify exceeded CC in these dimensions. Including these ensures success, reflecting that aquaculture operates within a socio-ecological system and promoting a holistic, participatory process involving stakeholders. Our study emphasizes integrating CC proxies into regulatory frameworks for aquaculture spatial planning and management. This, combined with the GIS-based application of CC calculations to AZAs identified in the Spanish Mediterranean (Fourdain et al., this issue), demonstrates these tools' applicability. For the first time, a CC proxy has been incorporated into a holistic and innovative framework balancing ecological, social, and economic considerations. Its simplicity and effectiveness make it a valuable tool for integrating into marine spatial planning and redefining AZAs with CC in mind. However, broader application necessitates adapting these proxies to various aquaculture systems. The socio-ecological heterogeneity across regions requires tailoring models to local conditions to harmonize spatial planning with environmental and socio-economic objectives effectively.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"608 ","pages":"Article 742756"},"PeriodicalIF":3.9,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plesiomonas-mediated biotransformation of Magnolia officinalis compounds in largemouth bass (Micropterus salmoides): A microbial strategy to optimize Chinese herbal medicine efficacy 银杏单胞菌介导的厚朴化合物在大口黑鲈体内的生物转化：优化中草药疗效的微生物策略

IF 3.9 1区农林科学

Aquaculture Pub Date : 2025-05-24 DOI: 10.1016/j.aquaculture.2025.742754

Xiaozhou Qi , Yongshuai Hu , Kai Shi , Yilin Zhang , Gaoxue Wang , Fei Ling

{"title":"Plesiomonas-mediated biotransformation of Magnolia officinalis compounds in largemouth bass (Micropterus salmoides): A microbial strategy to optimize Chinese herbal medicine efficacy","authors":"Xiaozhou Qi , Yongshuai Hu , Kai Shi , Yilin Zhang , Gaoxue Wang , Fei Ling","doi":"10.1016/j.aquaculture.2025.742754","DOIUrl":"10.1016/j.aquaculture.2025.742754","url":null,"abstract":"<div><div>Chinese herbal medicines (CHM), employed for millennia in therapeutic applications, exhibit complex chemical compositions that frequently compromise the bioavailability of their bioactive constituents. Emerging evidence underscores the pivotal role of gut microbiota in modulating CHM efficacy. Despite this progress, the specific microbial taxa responsible for these interactions—particularly in aquatic species—remain poorly characterized, hindering the development of microbiota-based aquaculture strategies. In this study, we systematically investigated key gut microbes that influence the uptake of magnolol and honokiol by largemouth bass (<em>Micropterus salmoides</em>). We demonstrated that: (i) antibiotic-induced depletion of gut microbiota significantly diminished serum and hepatic concentrations of magnolol and honokiol (<em>P</em> < 0.05), confirming microbial dependency in their pharmacokinetics; (ii) combined 16S rRNA sequencing and machine learning identified <em>Plesiomonas</em>, a core bacterial genus, as the dominant taxon correlated with enhanced absorption; (iii) in vitro fermentation of <em>Magnolia officinalis</em> crude extract with <em>P. shigelloides</em> M2 elevated magnolol and honokiol yields by 31.6 % and 24.4 %, respectively, compared to sterile controls; and (iv) in vivo trials confirmed that fermented extracts enhanced peak blood concentrations of magnolol (7.60 mg/L vs. 5.85 mg/L) and honokiol (2.29 mg/L vs. 1.71 mg/L) compared to non-fermented extracts. These findings demonstrate that <em>Plesiomonas</em> plays a central role in the biotransformation of CHM within the intestinal microbiota of aquaculture species. This discovery provides practical avenues for optimizing CHM bioavailability in aquaculture through strategic manipulation of gut microbial communities.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"608 ","pages":"Article 742754"},"PeriodicalIF":3.9,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144137742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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