Identification of an allotetraploid hybrid (Pengze crucian carp (♀) × Xingguo red carp (♂)) and its transcriptomic responses to herpesvirus infection

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-08-31 DOI:10.1016/j.aquaculture.2025.743138

Shujia Liao , Yingshi Tang , Han Wen , Honghai Liang , Guanghong Song , Jingfeng Liu , Mingqing Zhang , Zixuan E , Danqi Lu , Haoran Lin , Shuisheng Li , Yong Zhang

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引用次数: 0

Abstract

Compared with autopolyploids, allopolyploids derived from interspecific hybridization followed by genome doubling demonstrate superior agronomic performance through synergistic heterosis and polyploidy advantages. In this study, a hybrid was generated from Carassius auratus var. Pengze (♀) × Cyprinus carpio var. Xingguo (♂). Integrated analyses, including DNA content quantification, karyotyping, and single-copy nuclear gene profiling, confirmed that this hybrid was allotetraploid. Head-kidney histopathology at 1, 3, and 5 days post-infection (dpi) with C. auratus herpesvirus (CaHV) revealed that the number of lymphocytes decreased and that granulocyte infiltration intensified as the infection progressed. Transcriptomic analysis revealed that CaTRIM21 (tripartite motif-containing protein 21), CcTRIM21, CcPSCA (prostate stem cell antigen) and Capolyubiquitin were significantly upregulated at 1, 3, and 5 dpi, whereas CcCD209E (CD209 antigen-like protein E), CaCYP4501A1 (cytochrome P450 1A1) and CaCDH1 (cadherin-1) were significantly downregulated at 1, 3, and 5 dpi. Additionally, CccGAS (cyclic GMP-AMP synthase), CcLAAO (L-amino-acid oxidase), CaIL-11 (interleukin-11), CaIFN (interferon), CcIFN and CauPAR (urokinase-type plasminogen activator receptor) were significantly upregulated at 1 and 3 dpi. We suggest that the differentially expressed genes (DEGs) of the C. auratus and C. carpio subgenomes cooperate to resist virus invasion and establish a TRIM21-IFN-mediated defense at the early stage of infection. Trend analysis of the DEGs revealed that the DEGs of the C. auratus subgenome were involved in immune homeostasis (ECM-receptor, PI3K-Akt, MAPK, and PPAR pathways), whereas the DEGs of the C. carpio subgenome were highly involved in viral clearance (NK-cell cytotoxicity, antigen presentation, and complement activation). This study reveals the distinct roles of each subgenome in viral resistance, offering new insights into allopolyploid evolution and defense mechanisms.

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彭泽鲫鱼（♀）与兴国红鱼（♂）异体四倍体杂种的鉴定及其对疱疹病毒感染的转录组反应

与自体多倍体相比，通过种间杂交和基因组加倍获得的异源多倍体具有协同杂种优势和多倍性优势，表现出优越的农艺性能。本实验以鲫鱼（Carassius auratus var. Pengze（♀））与鲫鱼（Cyprinus carpio var. Xingguo（♂））为材料，进行杂交。综合分析，包括DNA含量定量，核型和单拷贝核基因分析，证实该杂种为异源四倍体。感染金弧菌疱疹病毒（CaHV）后1、3和5天的头肾组织病理学显示，随着感染的进展，淋巴细胞数量减少，粒细胞浸润增强。转录组学分析显示，CaTRIM21 （tripartite motif-containing protein 21）、CcTRIM21、CcPSCA（前列腺干细胞抗原）和Capolyubiquitin在1、3和5 dpi时显著上调，而CcCD209E （CD209抗原样蛋白E）、CaCYP4501A1（细胞色素P4501A1）和CaCDH1 （cadherin-1）在1、3和5 dpi时显著下调。此外，CccGAS（环GMP-AMP合成酶）、CcLAAO （l -氨基酸氧化酶）、CaIL-11（白细胞介素-11）、cafin（干扰素）、CcIFN和CauPAR（尿激酶型纤溶酶原激活物受体）在1和3 dpi时显著上调。我们认为，C. auratus和C. carpio亚基因组的差异表达基因（DEGs）协同抵抗病毒入侵，并在感染早期建立了trim21 - ifn介导的防御机制。deg趋势分析显示，C. auratus亚基因组的deg参与免疫稳态（ecm受体、PI3K-Akt、MAPK和PPAR通路），而C. carpio亚基因组的deg则高度参与病毒清除（nk细胞毒性、抗原呈递和补体活化）。这项研究揭示了每个亚基因组在病毒抗性中的独特作用，为异源多倍体的进化和防御机制提供了新的见解。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.