Aquaculture最新文献

{"title":"Augmentation of dmrt1 leads to gonadal differentiation towards testis in cyp17a1−/−;XX common carp","authors":"Shengchi Shi ,&nbsp;Xinyi Wang ,&nbsp;Jingyi Jia ,&nbsp;Guanghui Chen ,&nbsp;Hongfei Bao ,&nbsp;Xuehui Li ,&nbsp;Qiyong Lou ,&nbsp;Qingyun Yuan ,&nbsp;Jiangyan He ,&nbsp;Jian Wang ,&nbsp;Xueqiao Qian ,&nbsp;Gang Zhai ,&nbsp;Zhan Yin","doi":"10.1016/j.aquaculture.2026.743764","DOIUrl":"10.1016/j.aquaculture.2026.743764","url":null,"abstract":"<div><div>Although the essential role of estrogen in ovarian differentiation has been documented in several species of fish, this remains unknown in allotetraploid common carp (<em>Cyprinus carpio</em>). Here, the gonads from common carp with the <em>cytochrome P450 family 17 subfamily A member 1</em> (<em>cyp17a1</em>)^−/− genotype (knockout, KO), which exhibited complete testicular differentiation regardless of their genetic sex determination (GSD) system (XX or XY), were analyzed for transcriptional profiles at various stages during gonadal differentiation. At 25, 32 and 60 days post-hatching (dph), the <em>doublesex and mab-3 related transcription factor 1</em> (<em>dmrt1</em>)-<em>ovarian aromatase</em> (<em>cyp19a1a</em>) loop pattern could be clustered into high <em>dmrt1</em>-low <em>cyp19a1a</em> and <em>low dmrt1</em>-high <em>cyp19a1a</em> clusters, depending on the presence or absence of the <em>male-determining gene on the Y chromosome</em> (<em>mdy</em>) in genetic male and female <em>cyp17a1</em>^+/+ carp (Control, Con), respectively. The transcriptional profile of KO;XY carp continuously exhibited similar patterns to those of Con;XY carp in the <em>dmrt1</em>-<em>cyp19a1a</em> loop (high <em>dmrt1</em>-low <em>cyp19a1a</em>). However, the gene expression profiles of KO;XX carp deviated from those of Con;XX carp and skewed towards those of Con;XY and KO;XY carp from 25 to 60 dph, and clustered with those of Con;XY carp at 90 dph. At the same stage from 25 to 60 dph, Con;XX exhibited upregulated meiosis-related genes, including <em>synaptonemal complex central element protein 2</em> (<em>syce2</em>), <em>REC8 meiotic recombination protein b</em> (<em>rec8b</em>), and <em>DNA meiotic recombinase 1</em> (<em>dmc1</em>), among which only <em>dmc1</em> was observed to be upregulated in KO;XX carp. Administering 17β-estradiol restored ovarian differentiation in KO;XX carp. Antagonism between Dmrt1 and <em>cyp19a1a</em>/17β-estradiol was observed in fish gonads due to a direct interaction between Dmrt1 and Steroidogenic factor 1 (Sf-1), whereby Dmrt1 repressed Sf-1's ability to activate the <em>cyp19a1aA</em> and <em>cyp19a1aB</em> promoters. The Con;XX fish at 90 dph exhibited comprehensively advanced meiotic processes. We uncovered the genetic basis of a two-phase checkpoint regulatory mechanism of the putative <em>mdy</em> and estrogen sufficiency and elucidated the association between <em>dmrt1</em> and estrogen signaling in sustaining gonadal differentiation and the meiotic process in juvenile common carp during the period of 32–90 dph.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743764"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reconstructing Brazilian farmed shrimp export prices: A comparative time-series modelling approach","authors":"Vinícius Fellype Cavalcanti de França,&nbsp;Matheus Lourenço Soares Silva,&nbsp;Humber Agrelli Andrade","doi":"10.1016/j.aquaculture.2026.743753","DOIUrl":"10.1016/j.aquaculture.2026.743753","url":null,"abstract":"<div><div>Global seafood trade has expanded substantially in recent decades, making price dynamics a central element of fisheries and aquaculture economics. In Brazil, however, the shrimp farming sector faces structural constraints that have led to discontinuities in export price series, limiting empirical analyses and decision-support tools for producers, exporters, and policymakers. This study compares classical autoregressive models (ARIMA and ARIMAX) with machine learning approaches (Feedforward and Long Short-Term Memory neural networks) in reconstructing Brazilian farmed shrimp export prices. Model performance was evaluated using a rolling-origin, out-of-sample forecasting framework. Results indicate that the ARIMAX model, incorporating domestic farmed shrimp wholesale prices as an exogenous regressor, consistently outperformed all alternatives. Beyond lower forecast errors, ARIMAX effectively captured the dynamic interaction between domestic and export prices. In contrast, neural networks exhibited higher error variability and struggled to reproduce sharp price adjustments, particularly under limited training data. These findings suggest that parsimonious linear models enriched with economically meaningful market signals can outperform more complex machine learning architectures in data-constrained fisheries applications. The reconstructed export price series provides a valuable reference for market monitoring, risk assessment, and strategic planning, supporting price formation analysis, export competitiveness evaluation, and informed decision-making across the Brazilian shrimp supply chain.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743753"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Density-dependent effects of net-chasing training on aggression, stress, and metabolic load in the freshwater prawn Macrobrachium rosenbergii","authors":"Jiangtao Li,&nbsp;Jiale Zhou,&nbsp;Zhongjie You,&nbsp;Zheng Xiao,&nbsp;Licheng Zeng,&nbsp;Li Lin,&nbsp;Xiaojing Lv","doi":"10.1016/j.aquaculture.2026.743762","DOIUrl":"10.1016/j.aquaculture.2026.743762","url":null,"abstract":"<div><div>High culture density often intensifies social interactions, leading to frequent fighting, elevated physiological stress, and increased energy expenditure. In this study, we examined whether net-chasing training can alleviate these density-induced effects in freshwater prawn <em>Macrobrachium rosenbergii</em>. Prawns were reared in three stocking densities (40, 80, 120 individuals m⁻²) and two training modes (no chasing vs. 25%-exhausted net-chasing), with three replicates per treatment. Fighting behavior, inter-individual distance, appendage (second pereiopods, pleopods, uropods) activity, hemolymph lactate and cortisol, and key metabolic enzymes in locomotory muscles were quantified after 45 days, based on nine and three individuals per replicate for behavioral and physiological analyses, respectively. The results showed that, without net-chasing, increasing density revised in marked increases in fighting frequency, second pereiopods and pleopods beating, lactate and cortisol concentrations, and activities of glycolytic and aerobic enzymes in locomotory muscles, while inter-individual distance increased at 80 individuals m⁻² and collapsed at 120 individuals m⁻². Fighting indices were positively correlated with stress and metabolic indicators, indicating a close association among behavior, stress, and energy metabolism. At 80 individuals m⁻², net-chasing training significantly reduced aggression, appendage activity, stress indicators, and metabolic enzyme activities toward levels observed at low density (40 individuals m⁻²). In contrast, although training effects were still detectable at 120 individuals m⁻², they were markedly attenuated, and prawns remained in a high-stress, high-metabolism state. These results demonstrate that the mitigating effects of net-chasing training on density-induced responses are strongly density-dependent, with the strongest effects observed at high (but not extreme) stocking density.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743762"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apoptosis and FoxO signaling axis form the core antiviral defense in mud crab (Scylla paramamosain) against MCRV","authors":"Xiaoyu Wang ,&nbsp;Liangyu Chen ,&nbsp;Jing Peng ,&nbsp;Zixuan Luo ,&nbsp;Ming Zhang ,&nbsp;Qian Sun ,&nbsp;Ngoc Tuan Tran ,&nbsp;Xianliang Zhao ,&nbsp;Yueling Zhang ,&nbsp;Zhixun Guo ,&nbsp;Shengkang Li","doi":"10.1016/j.aquaculture.2026.743768","DOIUrl":"10.1016/j.aquaculture.2026.743768","url":null,"abstract":"<div><div>The mud crab (<em>Scylla paramamosain</em>) is an important economically farmed crustacean species in Southeast Asia, with continually increasing annual yields and high economic value. However, in recent years, the mud crab aquaculture industry has been afflicted by major disease outbreaks, among which Mud Crab Reovirus (MCRV) is the principal pathogen causing mass mortality of the species and posing a severe threat to industry development. In this study, transcriptomic analysis of mud crab hemocytes following MCRV infection was conducted to elucidate the molecular mechanisms underlying antiviral responses. The results revealed that MCRV infection significantly enriches apoptosis-related pathways, with widespread upregulation of genes, such as <em>p53</em>, <em>caspase-3</em>, <em>bax</em>, and <em>bag-1</em>. Functional experiments showed that promoting apoptosis effectively reduces viral loads, while inhibiting apoptosis accelerates viral replication. In addition, screening of immune signaling pathways showed that the transcription factor <em>SpFoxO</em> in the AMPK-FoxO pathway is markedly upregulated, and <em>Sp</em>FoxO regulates key genes of the RNAi pathway (including <em>dicer-2</em>, <em>ago2</em>, and <em>draper</em>) to enhance antiviral capacity. Silencing the <em>SpFoxO</em> gene led to increased viral load and decreased survival rate in mud crabs. In contrast, classical immune pathways such as TLR-Dorsal, IMD-Relish, and JAK-Stat showed weaker or reduced responses. This study provided evidence that mud crabs primarily rely on apoptosis and FoxO-mediated antiviral responses to combat MCRV infection, offering a theoretical basis for future antiviral intervention and disease management in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743768"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative analysis of transcriptome dynamics and Notch signaling pathways reveals molecular basis of egg quality in Plectropomus leopardus","authors":"Mingjian Liu ,&nbsp;Mengya Wang ,&nbsp;Chaofan Jin ,&nbsp;Jiahang Li ,&nbsp;Da Zheng ,&nbsp;Haizhan Tang ,&nbsp;Zhongyi Zhai ,&nbsp;Zhenmin Bao ,&nbsp;Bo Wang ,&nbsp;Jingjie Hu","doi":"10.1016/j.aquaculture.2026.743754","DOIUrl":"10.1016/j.aquaculture.2026.743754","url":null,"abstract":"<div><div>Egg quality is a critical determinant of embryonic survival and developmental competence in marine fish, directly affecting larval yield and aquaculture productivity. However, a comprehensive understanding of the molecular differences between eggs of varying quality during embryogenesis is still lacking. In this study, <em>Plectropomus leopardus</em> was selected as the research species to systematically compare high- and low-quality eggs (GE and LE) across three key embryonic stages, using a combination of microscopic phenotypic evaluation and time-series transcriptomic analysis. The results revealed a clear divergence between GE and LE in both morphological characteristics and transcriptomic profiles, with the most pronounced differences observed at the tailbud stage. GO and KEGG enrichment analyses indicated that inflammation- and immunity-related pathways (such as MAPK, TNF, IL-17, and Notch) were persistently activated in LE eggs, whereas GE eggs were predominantly enriched in pathways associated with lipid metabolism and energy supply. Weighted Gene Co-expression Network Analysis (WGCNA) analysis further identified co-expression modules strongly associated with egg quality and highlighted several key regulatory genes, including <em>ddit4</em>, <em>dusp1</em>, and <em>cebpd</em>. The validation of the Notch signaling pathway demonstrated that its downstream effectors exhibited dose-dependent expression responses to resveratrol treatment between 20 and 80 μM. Notably, moderate intervention effectively improved embryonic developmental status and enhanced both egg quality and embryo survival, further supporting the pivotal role of the Notch pathway in the regulation of egg quality. This study reveals the transcriptional regulatory landscape underlying egg quality differences, offering mechanistic insights into early marine fish development and supporting the identification of molecular markers for broodstock optimization and precision breeding.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743754"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suspect screening strategy reveals that illegal addition of drugs to fishery inputs is the main cause of drug residue problems in quality and safety of aquatic products in China","authors":"Hongyu Li ,&nbsp;Haiyan Zhang ,&nbsp;Zhongquan Jiang ,&nbsp;Guangxin Yang ,&nbsp;Cong Kong ,&nbsp;Yu Li ,&nbsp;Xiaosheng Shen","doi":"10.1016/j.aquaculture.2026.743741","DOIUrl":"10.1016/j.aquaculture.2026.743741","url":null,"abstract":"<div><div>Chinese-farmed aquatic products remain unqualified because of drug residues, even under standardized medication guidelines. This study examines actual aquaculture production activities in China and suspects that fishery inputs might be the main cause of this problem. Therefore, this study used ultrahigh-performance liquid chromatography/quadrupole–orbitrap mass spectrometry to screen and analyze drugs in fishery inputs, along with water, sediment, and organisms, which are used in 70 major aquaculture farms in Shanghai. The ecological risk of drug contamination in the aquaculture pond system was evaluated using the risk quotient (RQ) method to determine the relationship between the drug compositions of fishery inputs and drug residues in aquaculture organisms and the environment. The results showed that 18 drugs were screened out of the 489 samples. The number of drug species screened for fishery inputs, water, sediment, and organisms were 13, 16, 6, and 16, respectively. The highest concentrations were 1093.0 mg/kg, 1.245 μg/L, 37.45 μg/kg, and 29.47 μg/kg for fishery inputs, water, sediment, and organisms, respectively. Eleven of the sixteen drugs detected in the water-sediment-organism system were consistent with the detected species of fishery inputs, with an overlap rate of 68.8%, indicating that drug residues in aquaculture are closely related to the illegal addition of drugs to fishery inputs. The RQ method revealed that 47 (42.7%) were high-risk ponds, indicating that drug residues pose varying degrees of risk potential to the aquaculture organisms and environment. The study provides insight into the underlying causes of drug residues in aquatic products.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743741"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of sex inversion and feminization in the protandrous gilthead seabream (Sparus aurata) with 17β-estradiol implants, and its effects on egg production and quality","authors":"Katerina Samiotaki ,&nbsp;Maria Papadaki ,&nbsp;Irini Sigelaki ,&nbsp;Evangelia Manini ,&nbsp;Sabrin Hmida ,&nbsp;Pantelis Katharios ,&nbsp;Maja Ruetten ,&nbsp;Stelios Karapanagiotis ,&nbsp;Constantinos C. Mylonas","doi":"10.1016/j.aquaculture.2026.743740","DOIUrl":"10.1016/j.aquaculture.2026.743740","url":null,"abstract":"<div><div>The Mediterranean gilthead seabream (<em>Sparus aurata</em>) exhibits protandrous hermaphroditism, with all fish maturing first as functional 2-year-old males, and then 15–80% of these males invert to females prior to their second reproductive year, when 3 years old. For selective breeding programs, fish are selected to contribute to the next generation when they reach harvest size at 18–20 months of age (1+ years old, 400–600 g body weight, b.w.). At this time, all fish are males and cannot be predicted how many and which individuals will invert to females as 3-years old, complicating the management of selective breeding programs. Controlled-release 17β-Estradiol (E2) implants of 0.5 and 1 mg E2 kg⁻¹ b.w., administered in three monthly treatments (July–September) induced 89–100% feminization of 2 + −year-old males, compared to spontaneous feminization of only 20–25% in Controls. The resulting 3-year-old E2-feminized females underwent vitellogenesis successfully during the reproductive season and spawned eggs with slightly lower mean relative fecundities (15,200–27,100 eggs day⁻¹ kg⁻¹ b.w.), but equal fertilization (81–90%) and larval survival (85–94%) compared to the Controls (21,000–32,400 day⁻¹ kg⁻¹ b.w., 83–85% and 93–97%, respectively). Furthermore, fecundity, fertilization and larval survival of the produced eggs from these E2-feminized females a year later (4-year-old females) were similar to the previous year, and again within the published range of values for the species. The study produced an efficient E2-induced feminization protocol for gilthead seabream, which may facilitate breeding selection programs by eliminating the uncertainty for sex inversion success, thus allowing the reproduction of any selected 3-year-old gilthead seabream as a female.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743740"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review of the quantitative synthesis of shrimp farming benefits based on structural equation modeling","authors":"Yuzhen Wang ,&nbsp;Zhao Chen ,&nbsp;Jiajia Wang ,&nbsp;Zhiqiang Chang ,&nbsp;Zhitao Huang ,&nbsp;Shuangyong Zhang ,&nbsp;Gao Meng ,&nbsp;Jian Li","doi":"10.1016/j.aquaculture.2026.743765","DOIUrl":"10.1016/j.aquaculture.2026.743765","url":null,"abstract":"<div><div>Current studies on aquaculture systems predominantly emphasize the assessment of isolated economic or ecological indicators. This study employed an integrated framework combining life cycle assessment (LCA) and structural equation modeling (SEM) to evaluate the multidimensional sustainability of shrimp farming, including economic, ecological, and social dimensions, and conducted a comparative analysis of comprehensive benefit profiles across distinct aquaculture production models. The data were sourced from peer-reviewed literature published between 2006 and 2024, including empirical studies on <em>Litopenaeus vannamei</em> aquaculture systems that possessed quantifiable benefit indicators and complete data. Key indicators were extracted and coded for quantitative analysis. SEM was constructed, with economic, ecological, and social benefits as latent variables measured by observed indicators, and the standardized path coefficients from these latent variables to comprehensive benefits were estimated. Results indicated that economic benefits contributed the highest value to comprehensive benefits at 0.833, followed by social benefits (0.096), with the ecological benefits being the lowest (0.044). In terms of economic benefits, variable costs showed a negative correlation with the degree of intensification, whereas fixed costs were positively correlated, thus significantly correlating with the economic benefits. Among the ecological benefits, microbial diversity had a significant positive effect, whereas water quality factors and carbon dioxide emissions negatively affected the ecological benefits. Additionally, carbon sequestration has a significant positive effect on the ecological benefits (0.864), whereas carbon dioxide emissions are significantly negatively correlated with the ecological benefits (−0.129). In terms of social benefits, aside from the wages of temporary employees, the supply of shrimp, wages of permanent employees, and overall protein contribution rate all contribute positively. However, the microplastic content in shrimp (MPs-S) in the pond monoculture (PMC) model had a significant negative effect on the comprehensive benefits. In the indoor super-intensive culture (ISIC) model, carbon emissions had a significant negative effect on the ecological benefits, whereas microbial diversity had the greatest positive effect on the ecological benefits. This study proposes a method for objectively assessing and quantifying the comprehensive benefits of shrimp farming and provides a theoretical foundation for the green and sustainable development of the shrimp farming industry.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743765"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stocking density affects survival and gonad indices of sea urchins during commercial scale roe-enhancement","authors":"Rick Verkooijen,&nbsp;Fletcher Warren-Myers,&nbsp;Tim Dempster","doi":"10.1016/j.aquaculture.2026.743756","DOIUrl":"10.1016/j.aquaculture.2026.743756","url":null,"abstract":"<div><div>Roe-enhancement aquaculture can transform overabundant, malnourished sea urchins from barren reefs into a valuable seafood product. Numerous experimental scale studies show roe-enhancement is viable, but there are few commercial scale experiments, making commercial viability uncertain. We tested the effects of two diets and three density levels on urchin survival, and roe quality and quantity, during a 13-week commercial scale roe-enhancement trial. 6750 adult purple sea urchins (<em>Heliocidaris erythrogramma</em>) were hand collected from an urchin barren by divers into crates on the seafloor at three different densities: 40, 65 and 100 urchins m⁻². The crates were transported in seawater to an onshore rearing facility and placed in 3000 L tanks for the 13-week roe enhancement trial. Urchin mortality was low at 40 and 65 urchins m⁻² (3.3 and 5.4%, respectively), but significantly higher at 100 urchins m⁻² (11.5%). Densities above 40 urchins m⁻² reduced gonad index (GI) by 17%. The proportion of marketable urchins at 40 urchin m⁻² (63.2%) was higher than at 65 urchins m⁻² (46.5%), but not statistically different from 100 urchins m⁻² (49.2%). Diet type had no significant effect on gonad index, gonad quality or the marketability of the urchins. GIs and roe quality in all treatments exceeded market standards but were low compared to laboratory trials. Through careful transport and culture techniques, commercial scale roe-enhancement while ensuring acceptable mortality rates is viable, even at high densities, but differences observed in the GIs achieved by laboratory and commercial scale trials indicate substantial room for improvement.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743756"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecological safety evaluation of antibiotics of aquaculture importance through microalgal bioassay with Chlorella marina","authors":"Satheesha Avunje ,&nbsp;Cherian Tijo,&nbsp;Kizhakkekarammal Puthiyedathu Sandeep,&nbsp;Thangaraj Sathish Kumar,&nbsp;Priyadharshini Raju,&nbsp;Prasanna Kumar Patil","doi":"10.1016/j.aquaculture.2026.743734","DOIUrl":"10.1016/j.aquaculture.2026.743734","url":null,"abstract":"<div><div>Antibiotics are used for the effective management of bacterial infections in aquaculture. Judicious use of these substances is essential for ensuring environmental safety and sustainability. The present study reports the effect of important aquaculture antibiotics, oxytetracycline (OTC), sulfadimethoxine (SDM), florfenicol (FF), chloramphenicol (CP), furazolidone (FZ) and nitrofurazone (NF) on growth, photosynthetic activity and antioxidant enzyme levels in the non-target organism, <em>Chlorella marina</em>. The microalgae were exposed to 10, 30, 60, 90, 120 and 150 mg L⁻¹ and evaluated for growth, chlorophyll content, levels of antioxidants (catalase, superoxide dismutase and glutathione) and malondialdehyde at 24, 48, 72 and 96 h. The cytological damage following the exposure to IC₅₀ concentrations was evaluated under Scanned Electron Microscopy (SEM). The studied antibiotics are classified by toxicity level using the IC₅₀ values as follows: SDM &gt; NF &gt; FZ &gt; OTC &gt; CP &gt; FF. Growth inhibition of microalgal biomass, the content of chlorophyll <em>a</em> and b and anti-oxidative enzyme activity was dependent on the type of antibiotic and the proportion to the concentration and period of exposure. SEM images of microalgal cells exposed to antibiotics at IC₅₀ levels showed severe cellular deformation, aggregation and agglomeration. Results of the study suggest that at environmentally relevant concentrations, aquaculture important antibiotics have a little adverse impact on the growth, photosynthetic activity and antioxidant enzymes in the non-target organism, <em>Chlorella marina</em>. The variation in the effect of different antibiotics suggests the need for regulated monitoring on usage and environmental impact of these substances in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743734"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}