Aquaculture最新文献

{"title":"Effects of dietary bay leaf (Laurus nobilis) aqueous extract on growth performance, feed utilization, antioxidant activity, immunity, and gene expression in Nile tilapia (Oreochromis niloticus)","authors":"Akram Ismael Shehata ,&nbsp;Sara Ahmed Taha ,&nbsp;Ayaat M. Elmaghraby ,&nbsp;Ashraf I.G. Elhetawy ,&nbsp;Tarek Mohamed Srour ,&nbsp;Mohammed F. El Basuini ,&nbsp;Shimaa A. Shahin","doi":"10.1016/j.aquaculture.2025.742155","DOIUrl":"10.1016/j.aquaculture.2025.742155","url":null,"abstract":"<div><div>This study evaluated the effects of dietary <em>Laurus nobilis</em> leaf extract (LNLE) on growth performance, feed utilization, organ indices, histological structure, biochemical parameters, immune responses, and gene expression in Nile tilapia (<em>Oreochromis niloticus</em>). The experimental diets were nutritionally balanced, containing 31.27 % crude protein and 7.25 % crude lipid. After acclimatization, tilapia juveniles were randomly distributed into 18 hapas (6 treatments in triplicate) with 20 fish per hapa (initial weight: 6.04 ± 0.02 g). Over an 84-day trial, fish were fed diets containing LNLE at 0, 100, 200, 300, 400, and 500 mg/kg. Results showed significant improvements in growth metrics, including weight gain, specific growth rate, and feed conversion ratio, with optimal performance observed at 300 mg/kg LNLE. Histological analysis revealed dose-dependent changes in intestinal villi structure, facilitating nutrient absorption, while liver histology demonstrated better hepatic architecture and pancreatic acini at higher LNLE levels (400–500 mg/kg). Biochemical analyses indicated increased protein and globulin levels, reduced cholesterol, and enhanced immune responses, evidenced by elevated lysozyme activity and bacterial inhibition at a dosage of 200 mg/kg LNLE. Antioxidant enzyme activities (superoxide dismutase, catalase, and glutathione peroxidase) were significantly higher in LNLE-supplemented groups (200 mg/kg), with reduced lipid peroxidation. Gene expression analysis showed upregulation of <em>IGF-1</em> and <em>GLUT4</em> and downregulation of <em>TNF-α</em>, highlighting LNLE's role in modulating growth and immune-related pathways. In conclusion, LNLE supplementation enhances growth, feed efficiency, immune function, and antioxidant activity in Nile tilapia, demonstrating its potential as a natural growth promoter in aquaculture. Future research should optimize LNLE dosage, assess its long-term effects on health and sustainability, and explore its potential in enhancing disease resistance in commercial aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742155"},"PeriodicalIF":3.9,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143292593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xanthosine-mediated modulation of Haematococcus pluvialis growth and stress response under high light: Insights from physiological and transcriptome analysis","authors":"Kai Liu,&nbsp;Rongrong Lv,&nbsp;Hailiang Xing,&nbsp;Xue Sun,&nbsp;Nianjun Xu,&nbsp;Chaoyang Hu,&nbsp;Liuquan Zhang","doi":"10.1016/j.aquaculture.2025.742156","DOIUrl":"10.1016/j.aquaculture.2025.742156","url":null,"abstract":"<div><div><em>Haematococcus pluvialis</em> always forms a thick and rigid secondary cell wall (SCW) during astaxanthin accumulation under high light conditions, which poses significant challenges for the efficient recovery of bioactive products. Cultivating motile cells that lack SCW offers a potential solution to this issue. In this study, it was demonstrated that enhanced purine metabolism can inhibit SCW formation in <em>H. pluvialis</em>. Exogenous supplementation with 1 mM xanthosine increased the proportion of motile cells from nearly 0 % to 93.2 % after nine days of high light exposure. Additionally, xanthosine treatment led to increased cell density, enhanced chlorophyll fluorescence parameters, elevated starch, and protein content, and reduced reactive oxygen species (ROS), carbohydrate, and lipid levels. Moreover, xanthosine increased the contents of unsaturated and total fatty acids on day 3 but decreased these levels on day 9. Comparative transcriptome analysis revealed that xanthosine significantly upregulated the expression of genes involved in purine metabolism, cell cycle regulation, carbon fixation in photosynthesis, photosynthesis-antenna proteins, glycolysis, TCA cycle, amino acid biosynthesis, unsaturated fatty acid biosynthesis, starch biosynthesis, and astaxanthin biosynthesis. Conversely, it significantly downregulated the expression of genes associated with mannan biosynthesis, algaenan biosynthesis, and the methylerythritol 4-phosphate (MEP) pathway. Additionally, xanthosine significantly altered the expression of genes involved in the antioxidant system and the MAPK signaling pathway. Collectively, this study establishes a link between purine metabolism and SCW synthesis in <em>H. pluvialis</em>, and provides insights into the mechanism by which xanthosine influences growth and SCW synthesis under high light conditions.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742156"},"PeriodicalIF":3.9,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginseng extract alleviates growth reduction, intestinal barrier damage, and oxidative stress in Lithobates catesbeiana induced by soybean meal","authors":"Xiongqi Wu ,&nbsp;Yongquan Han ,&nbsp;Bo Zhu ,&nbsp;Xu Shi ,&nbsp;Yi Hu ,&nbsp;Jihong Dai","doi":"10.1016/j.aquaculture.2025.742154","DOIUrl":"10.1016/j.aquaculture.2025.742154","url":null,"abstract":"<div><div>The present study investigated the effects of ginseng extract on growth performance, antioxidant capacity, and intestinal health in bullfrogs fed high soybean meal diets. A total of four iso‑nitrogenous diets (crude protein, 40.00 %) and isolipidic diets (crude lipid, 8.00 %) were formulated and administered over a period of 8 weeks. A basal diet containing 10 % fishmeal (FM), a diet replacing all fishmeal with soybean meal (SBM), SBM supplemented with 0.05 % (GSP1) and 0.10 % (GSP2) ginseng extract. Compared to the SBM diet, the inclusion of dietary GSP2 notably alleviated the decline in growth performance and feed efficiency caused by soybean meal (<em>P</em> &lt; 0.05). The levels of total protein and albumin in the serum of the GSP2 group were significantly increased, while the activities of alanine aminotransferase and aspartate aminotransferase showed a marked decrease (<em>P</em> &lt; 0.05); The GSP2 group exhibited a significant increase in the activities of digestive enzymes, particularly lipase and trypsin, when compared to the SBM group. (<em>P</em> &lt; 0.05). Antioxidant indices within the liver, encompassing catalase, superoxide dismutase, reduced glutathione exhibited significant improvements in both the GSP1 and GSP2 groups (<em>P</em> &lt; 0.05). Diet with ginseng extract supplementation significantly improved intestinal morphology, decreased the gene expression of intestinal pro-inflammatory cytokines (<em>P</em> &lt; 0.05). There was a significant shift in microbial populations, favoring beneficial bacteria such as <em>Akkermansia</em> and <em>Sphinomonas</em> in the GSP groups. In conclusion, the supplementation of ginseng extract effectively mitigates the adverse impacts of high soybean meal diets on bullfrog growth, liver health, and intestinal function, while simultaneously enhancing immune response and antioxidant capacity. Notably, optimal effects were observed at a supplementation level of 0.10 %.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742154"},"PeriodicalIF":3.9,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth and survival of larvae of the threatened estuarine clam, Polymesoda arctata, under different hatchery conditions","authors":"Cindi Guete-Salazar ,&nbsp;Judith M. Barros ,&nbsp;Katherina Brokordt ,&nbsp;Luz Adriana Velasco","doi":"10.1016/j.aquaculture.2025.742158","DOIUrl":"10.1016/j.aquaculture.2025.742158","url":null,"abstract":"<div><div>The estuarine clam <em>Polymesoda arctata</em> is one of the most important artisanal fisheries mollusks in the Caribbean Sea and is currently threatened by overexploitation and habitat loss. In order to provide a basis for aquaculture production of this species as a tool for its conservation and sustainable use, the growth and survival of its embryos and larvae were evaluated under different hatchery culture conditions. An embryo culture experiment was conducted at different incubation densities (1, 5, 10, or 15 zygotes mL⁻¹). In addition, five larval culture experiments were performed testing different culture densities (1 or 5 larvae mL⁻¹), food rations (20 or 40 cells of <em>Isochrysis galbana</em> μL⁻¹ d⁻¹), temperatures (25, 27, or 29 °C), salinities (5, 15, or 20 ppt), and water exchange (total or partial, every 48 h). Density affected the survival and growth of embryos, with the highest values observed at intermediate densities. Density, food ration, and water exchange did not affect larval survival but did affect their growth. Growth was higher in larvae maintained at low density levels, low food rations, and with total water exchange. Larvae maintained at intermediate and high temperatures also showed higher survival and growth rates. Finally, medium and low salinities favored larval survival, while intermediate and high salinities benefited growth. The high average values of survival (50 %) and growth rate (9 μm d⁻¹) obtained in this study, along with reaching the competent larval stage in just 11 days, allow us to affirm that this clam has a high potential for hatchery production, especially when maintained at intermediate or low culture densities (5 zygotes per mL⁻¹ and 1 larva per mL⁻¹), low food rations (20 cells of <em>I. galbana</em> μL⁻¹ d⁻¹), intermediate to high temperatures (between 27 and 29 °C), intermediate salinity (15 ppt), and with total water exchange. This work lays the foundational work framework for larval production of <em>P. arctata</em> in hatcheries, a crucial step for its cultivation and restocking efforts.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742158"},"PeriodicalIF":3.9,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143292600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SHP-1 interacts with NFκB1 to inhibit its phosphorylation and nuclear translocation to exert its anti-bacterial function","authors":"Ningning Wang ,&nbsp;Suxu Tan ,&nbsp;Muyuan Wang ,&nbsp;Hongning Liu ,&nbsp;Sen Han ,&nbsp;Zhendong Wu ,&nbsp;Jie Ma ,&nbsp;Songlin Chen ,&nbsp;Zhenxia Sha","doi":"10.1016/j.aquaculture.2025.742148","DOIUrl":"10.1016/j.aquaculture.2025.742148","url":null,"abstract":"<div><div>The protein tyrosine phosphatase SHP-1 plays pivotal roles in maintaining cellular homeostasis, proliferation, and differentiation. Although SHP-1 has been shown to have anti-inflammatory effects in mammals, its potential anti-bacterial effects and regulatory mechanisms have not been elucidated in teleost. Chinese tongue sole (<em>Cynoglossus semilaevis</em>), an economically important aquaculture flatfish, has been widely used as a genomics and disease model. Herein, using this fish species, we demonstrated the function of SHP-1 <em>in vitro</em> and <em>in vivo</em> and further revealed the underlying mechanism in immune response caused by <em>Vibrio anguillarum</em>. Overexpression of SHP-1 decreased mortality and alleviated histopathological deterioration of Chinese tongue sole, while its inhibition exhibited the opposite effects. Accordingly, SHP-1 inhibited the activation/transduction of the toll like receptor 5-myeloid differentiation primary response 88-nuclear factor kappa-B (TLR5-MYD88-NFκB) axis and the secretion of pro-inflammatory cytokines. Most importantly, we revealed for the first time that SHP-1 inhibits Nuclear Factor Kappa B Subunit 1 (NFκB1) phosphorylation and nuclear translocation by interacting with NFκB1, thereby suppressing the activation of NFκB signaling and blocking excessive inflammation caused by bacteria and lipopolysaccharide (LPS). In summary, our findings systematically dissect the functions of SHP-1 in bacterial inflammation using Chinese tongue sole, and provide mechanistic insights into the control of bacterial diseases in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742148"},"PeriodicalIF":3.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of the salmon lice density on wild sea trout from the mean predicted lice density in the sea: A cross-validation of the data in Myksvoll et al. (2018)","authors":"Bjarne Gjerde ,&nbsp;Muhammad Luqman Aslam","doi":"10.1016/j.aquaculture.2025.742149","DOIUrl":"10.1016/j.aquaculture.2025.742149","url":null,"abstract":"<div><div>The coast of Norway is divided into 13 production zones (PZ 1–13) for farmed Atlantic salmon and rainbow trout. In the “Traffic Light System” (TLS) the increased mortality risk due to lice infestation on wild migrating smolt determines whether the production at the grow-out farms in a PZ must be reduced (Red light), can remain at the current level (Yellow), or can be increased (Green). <span><span>Myksvoll et al. (2018)</span></span> analysed observed mean lice burden per gram body weight (OMLB) of wild sea trout caught using traps and nets at 102 locations each of grid size 15 × 15 grid; i.e., 12 km × 12 km =144 km²) along the coast and the predicted mean lice particle density in the sea (PMLD) at these locations.. They concluded, based on a Spearman rank correlation of 0.72 between log of the PMLD and OMLB values, that PMLD provide reliable information about the spatial distribution of salmon lice infestation pressure all along the coast and therefore of importance for the knowledge base of TLS. Cross-validation of their data with linear regression models, bivariate logistic regression models, and a threshold model with three assumed mortality classes (Green, Yellow and Red), do not confirm their conclusions. Rather, the very low predictive power of all models shows that PMLD is such a poor predictor of OMLB that assigning a Green, Yellow or Red colour to a PZ with a sufficiently high statistical power is only possible with PMLD values from many sea areas in sum larger than the area of the majority of the 13 PZs.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742149"},"PeriodicalIF":3.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143292594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of total ammonia and nitrite removal, growth, and vibrio tolerance in whiteleg shrimp (Penaeus vannamei) by Paracoccus marcusii ND07 administration in rearing water","authors":"Wei Wang ,&nbsp;Jian Li ,&nbsp;Siyi Liang ,&nbsp;Mingliang Chen ,&nbsp;Jiabao Guo ,&nbsp;Lu Wang ,&nbsp;Zhenyue Lin ,&nbsp;Jianming Chen","doi":"10.1016/j.aquaculture.2025.742152","DOIUrl":"10.1016/j.aquaculture.2025.742152","url":null,"abstract":"<div><div>Managing nitrogenous pollutants is a critical issue in the field of aquaculture production. A heterotrophic bacterial strain, <em>Paracoccus marcusii</em> ND07, possessing the capability to remove both total ammonia nitrogen (TAN) and nitrite, was isolated from shrimp culture water. The bacterial isolate was found to be non-toxic and susceptible to a broad spectrum of antibiotics. The impact of ND07 bacterial suspensions on the aquaculture of <em>Penaeus vannamei</em> was investigated. Over an eight-week period, shrimp rearing water was treated with three different concentrations of ND07: 10⁴ (T1), 10⁵ (T2), and 10⁶ (T3) CFU/mL, along with a control group devoid of ND07. The introduction of ND07 in all treatment groups resulted in a significant decrease in the concentrations of TAN and nitrite, along with a reduction in the counts of <em>Vibrio</em> spp. in the culture water. When compared to the control, the treated groups exhibited significantly enhanced growth performances, as evidenced by the final weight, weight gain, and specific growth rate. Notably, the feed conversion ratio was substantially improved in T2 and T3 groups. While the application of ND07 did not significantly affect survival rates, it exhibited pronounced improvements in immune and antioxidant parameters. This was evidenced by elevated activities of Phenoloxidase, Lysozyme, and Catalase in the shrimp plasma. Subsequent to the treatment trial, a challenge experiment with <em>Vibrio vulnificus</em> revealed a statistically significant increase in survival rates among the infected shrimp that received ND07 treatments, in comparison to the untreated control group. In addition, shrimp in the treatment groups showed significantly decreased histological changes of the hepatopancreas organ caused by vibrio infection. These results suggest that the administration of ND07 in shrimp rearing water confers beneficial effects for shrimp aquaculture. The application of ND07 at densities of 10⁵ and 10⁶ CFU/mL not only facilitated a reduction in TAN and nitrite concentrations, but also promoted growth performance in shrimp. Furthermore, a concentration of 10⁶ CFU/mL of ND07 was particularly effective in enhancing immune and antioxidant enzymatic activities, as well as boosting resistance to diseases caused by pathogenic <em>Vibrios</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742152"},"PeriodicalIF":3.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-invasive detection method for Bonamia ostreae infected Ostrea edulis","authors":"Tim Regan ,&nbsp;Lavanya Vythalingam ,&nbsp;Jennifer Nascimento-Schulze ,&nbsp;Owen Paisley ,&nbsp;Alain Karmitz ,&nbsp;Nuala M. Hanley ,&nbsp;William G. Sanderson ,&nbsp;Tim P. Bean","doi":"10.1016/j.aquaculture.2025.742153","DOIUrl":"10.1016/j.aquaculture.2025.742153","url":null,"abstract":"<div><div>Populations of the native European flat oyster (<em>Ostrea edulis</em>) have been in decline for the past two centuries through a combination of exploitation, disease and climatic events. Ongoing efforts to restore these animals to suitable habitats throughout Europe are increasing in number and magnitude but have been hindered by pathogens such as the protist parasite <em>Bonamia ostreae</em>. Detecting such pathogens in animals before relocation to disease-free restoration or farm sites remains a major issue. Current detection methods rely on sacrificially dissecting a subset of the animals to perform qPCR and histology. While very sensitive, this method is destructive and the animals which are moved are themselves untested. Here, we present a non-invasive scalable method to detect <em>Bonamia ostrea</em> in <em>O. edulis</em>. Following overnight quarantine in aerated seawater, faeces and pseudofaeces produced by the oysters is collected. The DNA is extracted from this material and analysed by qPCR for presence of the pathogen. This approach proves more sensitive for pathogen detection than eDNA sampling from the water alone. Furthermore, when tested alongside conventional histology and tissue-extracted DNA qPCR results, our method demonstrated comparable sensitivity levels. Future studies aim to adapt this method to detecting other aquatic diseases or invasive species but it should be noted that this may be a pathogen-specific characteristic.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742153"},"PeriodicalIF":3.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic evaluation of nutritional traits in Malabar red snapper (Lutjanus malabaricus): Heritability and genetic correlations of fatty acid composition","authors":"Kathiresan Purushothaman ,&nbsp;Nguyen Thanh Vu ,&nbsp;Saraphina Dianne Tneo Rwei Qing ,&nbsp;Joyce Koh ,&nbsp;Muhammad Hazim Bin Mohamed ,&nbsp;Rachel Ho Jia Wen ,&nbsp;Bing Liang ,&nbsp;Grace Loo ,&nbsp;Jose A. Domingos ,&nbsp;Dean R. Jerry ,&nbsp;Shubha Vij","doi":"10.1016/j.aquaculture.2025.742144","DOIUrl":"10.1016/j.aquaculture.2025.742144","url":null,"abstract":"<div><div>The demand for nutritious seafood highlights aquaculture's role in global food security, with selective breeding now commonplace in many species as an approach to increase productivity and quality of farmed species. The Malabar red snapper (<em>Lutjanus malabaricus</em>) is a high-value marine species where there is interest in selective breeding to improve commercial traits. In this study genetic parameters associated with fatty acid composition were investigated in Malabar red snapper muscle fillet to understand if these traits would respond to genomic selection. The fatty acid profiles of 540 fish from a commercial farm in Singapore were analysed focusing on key components such as omega-3 and omega-6 fatty acids. A genomic relationship matrix, generated using 39,780 high-quality SNPs from a custom 70 K SNPs red snapper array, was employed in a linear mixed model to reveal heritable components of 15 fatty acid-related traits. Heritability for fatty acids ranged from 0.15 to 0.50. Polyunsaturated fatty acids (PUFA), n-3 omega fatty acids such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) also showed moderate heritability of 0.32 and 0.29, respectively. DHA/EPA (<em>h</em>^<em>2</em> = 0.50) and the sum of EPA and DHA (<em>h</em>^<em>2</em> = 0.46) showed especially moderate-high heritability. High genetic correlations between levels of fatty acids were observed, including alpha-linolenic acid (ALA) with arachidonic acid (ARA; <em>r</em>_<em>g</em> = 0.90) and Oleic acid (OA; <em>r</em>_<em>g</em> = 0.82). This study demonstrated that variation in fatty acid levels are heritable and thus would respond to selection. The findings highlight the potential to enhance the nutritional quality of Malabar red snapper fillets through selective breeding, contributing to product quality and better health benefits for consumer.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742144"},"PeriodicalIF":3.9,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143292625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accuracies of genomic prediction accounting for genotype by environment remain high when using small sets of selected SNPs in barramundi Lates calcarifer","authors":"Cecile Massault ,&nbsp;David B. Jones ,&nbsp;Marie Lillehammer ,&nbsp;Holly Cate ,&nbsp;Paul Harrison ,&nbsp;Jan M. Strugnell ,&nbsp;Kyall R. Zenger ,&nbsp;Nicholas A. Robinson ,&nbsp;Dean R. Jerry","doi":"10.1016/j.aquaculture.2025.742138","DOIUrl":"10.1016/j.aquaculture.2025.742138","url":null,"abstract":"<div><div>Barramundi (<em>Lates calcarifer</em>) is an important tropical aquaculture species gaining popularity in farming and benefiting from genetic and genomic tools to achieve genetic improvement. However, the mass spawning behaviour of the species leads to disproportionate broodfish contribution and family representation, requiring genotyping of many fish for selection practices. Genotyping at an industrial-scale remains costly, hindering the development of aquaculture breeding programs, especially if high-density solid-state array-based platforms are applied. One strategy to reduce cost is using lower density panels while maintaining high accuracy of predictions. To assess the feasibility of lower-density SNP genotyping panels for genomic prediction of body weight in barramundi, the prediction accuracy of pedigree and genomic BLUP based on an Axiom panel of ∼52,000 (52 K) SNP was compared to four lower-density SNP scenarios; a) panels ranging from 2000 (2 K) to 25,000 (25 K) SNPs either randomly selected, or selected based on ranked effect size using single step GWAS (ssGWAS) for body weight for two grow-out systems (freshwater RAS and brackish water ponds); b) a custom-made 5000 (5 K) SNP panel, comprising SNPs selected for parentage assignment (1000 SNP), as well as 4000 randomly chosen SNPs from the 52 K after quality control filtering; c) a 5 K SNP panel selected based on largest marker effect size for body weight but used to evaluate genomic predictions in other commercially important traits to assess the possibility to use a single panel for all traits and finally; d) a 5 K SNP panel selected based on effect size from ssGWAS in two grow-out systems.</div><div>Genomic predictions under different scenarios confirmed a distinct relationship between panel size and prediction accuracy estimated with 10-fold cross validation. As the size of the SNP panel decreases from 25 K to 2 K (by selecting only trait-associated SNPs with large effects), prediction accuracy increased from 0.53 (±0.12) to 0.81 (±0.07) within a single trait (body weight) in a single environment. However, these panels did not consistently perform well with other traits, such as body shape and Fulton's condition factor K (0.41 ± 0.17 and 0.27 ± 0.19, respectively), and were only as accurate as the Reference (52 K) SNP panel when individuals from two different environments were included (0.62 ± 0.06).</div><div>In conclusion, using optimized panels is cost-effective and a high accuracy of prediction is retained when focusing on a single trait in a single environment. However, custom-made panels were found to be more versatile when selecting different traits in different environments.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742138"},"PeriodicalIF":3.9,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}