Aquaculture最新文献

{"title":"Dietary protease inclusion with activity after pelleting affects growth performance, digestive enzyme activity, nutrient sensing signaling pathway and intestinal health of larval largemouth bass (Micropterus salmoides)","authors":"Nihe Zhang ,&nbsp;Ye Gong ,&nbsp;Runwei Miao ,&nbsp;Zeqiang Sun ,&nbsp;Sen Zhang ,&nbsp;Xuxiong Huang ,&nbsp;Naisong Chen ,&nbsp;Songlin Li","doi":"10.1016/j.aquaculture.2025.742581","DOIUrl":"10.1016/j.aquaculture.2025.742581","url":null,"abstract":"<div><div>A 32-day feeding trial was conducted to explore the effects of dietary protease (with activity after pelleting) on growth performance, digestive enzyme activity, nutrient-sensing signaling pathways and intestinal health of larval largemouth bass. Four isonitrogenous and isolipidic micro-diets were formulated with graded levels of protease (neutral protease: keratinase = 2:1) at 0 % (PE-0), 1 % (PE-1), 2 % (PE-2), and 3 % (PE-3). The larvae were fed these diets to apparent satiety four times daily with three replicates. In the present study, the exogenous enzymes have been confirmed to function after pelleting. Dietary protease inclusion linearly reduced the deformity rate and enhanced the growth performance of largemouth bass larvae, with no significant difference was observed in growth between the PE-2 and PE-3 groups. The activities of digestive enzymes also increased linearly, with no significant differences between the PE-2 and PE-3 groups. Furthermore, protease supplementation resulted in a linear increase in the gene expressions of gastrointestinal peptides and amino acid transporters, with the highest expression of <em>lat1</em> and <em>lat2</em> observed in the PE-2 group. Meanwhile, dietary protease inclusion linearly elevated the TOR pathway by activating <em>tor</em> and <em>akt1</em>, with no significant differences between the PE-2 and PE-3 groups, while it also restricted <em>4ebp1</em>. Protease inclusion also linearly enhanced the gene expressions of intestinal tight junction proteins (<em>claudin4</em> and <em>ocln</em>). Moreover, dietary protease inclusion linearly inhibited the expression of pro-inflammatory factors (<em>p38β-mapk11</em>, <em>p38α-mapk14</em>, <em>rela</em>, <em>tnf-α</em>, <em>il-1β</em>, and <em>il-8</em>) and increased the expression of the anti-inflammatory factor <em>tgf-β1</em>, with no significant differences observed between the PE-2 and PE-3 groups. Additionally, protease inclusion elevated the expression of the non-specific immune factor <em>lysozyme</em>, with the highest value observed in the PE-2 group. Overall, the inclusion of protease with activity after pelleting improved the growth and development of larval largemouth bass, and the inclusion dose of 2 %–3 % was recommended for the micro-diet.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742581"},"PeriodicalIF":3.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of novel bacteriophage as a promising alternative for controlling Vibrio infections in shrimp","authors":"Hua Xu ,&nbsp;Wenxin Li ,&nbsp;Yijing Zhang ,&nbsp;Mingxu Li ,&nbsp;Xuan Dong ,&nbsp;Song Zhang ,&nbsp;Jie Huang ,&nbsp;Juhee Ahn","doi":"10.1016/j.aquaculture.2025.742579","DOIUrl":"10.1016/j.aquaculture.2025.742579","url":null,"abstract":"<div><div><em>Vibrio parahaemolyticus</em> is a significant pathogen affecting shrimp aquaculture, leading to substantial economic losses. This study aimed to isolate and characterize <em>Vibrio</em> phage with potential applications in controlling <em>V. parahaemolyticus</em> infections in shrimp. We isolated specific bacteriophages targeting <em>V. parahaemolyticus</em> from 61 shrimp samples collected from shrimp ponds. Among them, <em>Vibrio</em> phage P20211219001–1 was selected for characterization of its biological and morphological properties. The isolated <em>Vibrio</em> phage showed high host specificity, forming clear plaques on <em>V. parahaemolyticus</em> P20211219001–1, but no plaques on other tested <em>Vibrio</em> strains. The <em>Vibrio</em> phage P20211219001–1 exhibited an optimal multiplicity of infection (MOI) of 0.001, with a latent period of 20 min, a burst period of 30 min, and a burst size of 23 PFU/cell. Stability tests revealed that <em>Vibrio</em> phage P20211219001–1 remained stable at temperatures between 4 °C and 50 °C and across a broad pH range of 3 to 11. Morphologically, the phage featured a hexagonal head (83 nm) and a short tail (24 nm), belonging to the order <em>Caudoviricetes</em>. Genomic analysis of <em>Vibrion</em> phage revealed a 35,961 bp circular dsDNA genome with 41 predicted protein-coding sequences, including genes involved in phage structure, DNA replication, and host bacterial lysis. Lytic proteins identified include holins and <em>N</em>-acetylmuramoyl-<em>L</em>-alanine amidase. In application trials, <em>Vibrio</em> phage P20211219001–1 demonstrated significant protective effects against <em>V. parahaemolyticus</em> infection in <em>Artemia salina</em> and <em>Litopenaeus vannamei</em>, improving survival rates by 20–37 % compared to control. The results suggest that newly isolated <em>Vibrio</em> phage P20211219001–1 showed great potential for application in seafood safety, providing practical strategy for controlling <em>V. parahaemolyticus</em> infections.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742579"},"PeriodicalIF":3.9,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic selection for hard-to-measure traits in aquaculture: Challenges in balancing genetic gain and diversity","authors":"Ziyi Kang ,&nbsp;Jie Kong ,&nbsp;Qi Li ,&nbsp;Juan Sui ,&nbsp;Ping Dai ,&nbsp;Kun Luo ,&nbsp;Xianhong Meng ,&nbsp;Baolong Chen ,&nbsp;Jiawang Cao ,&nbsp;Jian Tan ,&nbsp;Qiang Fu ,&nbsp;Zhaoxin Wang ,&nbsp;Sheng Luan","doi":"10.1016/j.aquaculture.2025.742576","DOIUrl":"10.1016/j.aquaculture.2025.742576","url":null,"abstract":"<div><div>Genomic selection (GS) is being actively evaluated in aquaculture for its enhanced prediction accuracy over traditional pedigree-based selection (PS). However, the long-term efficiency of GS for the hard-to-measure trait in family-based aquaculture breeding programs remains unclear. This study simulated a typical family-based breeding program for Pacific white shrimp, focusing on a hard-to-measure trait with low heritability (0.10), to investigate genetic gain, genetic diversity, and conversion efficiency (CE) at the genome level. The impacts of panel density, reference group size, and genotype imputation on these metrics were assessed. GS increased genetic gain by 15.57 % to 113.29 % compared to PS when using panel densities of 12 to 1250 SNPs per chromosome. However, GS reduced genetic diversity by 6.36 % to 41.23 %, and decreased CE by 16.54 % to 57.60 % relative to PS, primarily due to poor within-family prediction accuracy, which biased the selection of candidates and the optimization of mating plans. When the within-family prediction accuracy reached 0.7, the CE of GS became comparable to that of PS. Genotype imputation improved CE in GS, particularly at panel densities of 3 to 23 SNPs per chromosome. Genotype imputation increased CE by 23.04 % to 158.72 % compared to non-imputed GS and by 9.70 % relative to PS. The reference group size and panel density exhibited inflection points in their impacts on the genetic gain and CE of GS. Beyond thresholds (panel density: 114 SNPs per chromosome; reference group size: 70 individuals per family), additional resource allocations resulted in significantly diminishing returns. <!--> <!-->Moreover, the inflection point for panel density was more pronounced than that for reference group size, as evidenced by <!--> <!-->the lack of significant CE improvements in GS when reference group sizes expanded from 30 to 200 individuals per family. Our findings highlight that managing within-family diversity in GS poses significant challenges, which negatively affect CE and the sustainability of breeding schemes. Nevertheless, low-density SNP panels combined with genotype imputation offer a cost-effective and practical strategy for achieving superior genetic gains while maintaining sustainability. Breeders should prioritize optimizing panel density when implementing GS under resource-limited conditions.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742576"},"PeriodicalIF":3.9,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating lighting intensity and angle to facilitate feeding in paralarvae of the common Sydney octopus, Octopus tetricus","authors":"Jeffrey Good ,&nbsp;Andrew Jeffs ,&nbsp;Shigeki Dan ,&nbsp;Stefan Spreitzenbarth","doi":"10.1016/j.aquaculture.2025.742560","DOIUrl":"10.1016/j.aquaculture.2025.742560","url":null,"abstract":"<div><div>Efforts to establish sustainable octopus aquaculture are hindered by high mortality of octopus paralarvae during larviculture. The optimum culture conditions in which paralarvae can best visualize and capture food items for nutrient delivery remains undescribed. This study investigated the ingestion of prey by <em>Octopus tetricus</em> paralarvae, aged 2 days post hatch, under different combinations of light intensity (600 lx or 15 lx) and light angle (0°, 45°, or 90°). Groups of ten octopus paralarvae were allowed to feed on 3000 <em>Artemia</em> under one of the six lighting combinations for 6 h, whereafter intact or partially ingested <em>Artemia</em> were counted. The greatest total number of <em>Artemia</em> ingested for all paralarvae in a lighting treatment was 279 <em>Artemia</em> under a high intensity light sourced from 90° (<em>P</em> &lt; 0.001). This total number was 77 % greater than the second most successful light treatment, low intensity light sourced from 90° (<em>P</em> &lt; 0.001) and 675 % greater than the least successful light treatment, low intensity light sourced from 45° (<em>P</em> &lt; 0.001). Prey ingestion by individual paralarva was six times greater under high intensity light sourced from 90° than under low intensity light sourced from 45°. Examination of <em>Artemia</em> remains suggested paralarvae captured <em>Artemia</em> in regions of high body surface area, providing information for improved nutrient delivery. Under the best lighting, paralarvae would have fully ingested more <em>Artemia</em> suggesting that feeding efficiency is highly dependent on improved prey visibility. Future rearing efforts should consider these essential factors of light to optimise feeding success with octopus paralarvae.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742560"},"PeriodicalIF":3.9,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ctenopharyngodon idella (grass carp) SIRT7 negatively regulates anti-GCRV innate immunity by promoting IRF3 and IRF7 degradation","authors":"Yao Bai ,&nbsp;Qian Liao ,&nbsp;Zixuan Wang ,&nbsp;Chunchun Zhu ,&nbsp;Wen Liu ,&nbsp;Wuhan Xiao ,&nbsp;Xing Liu","doi":"10.1016/j.aquaculture.2025.742575","DOIUrl":"10.1016/j.aquaculture.2025.742575","url":null,"abstract":"<div><div><em>Ctenopharyngodon idella</em> (grass carp) is widely regarded as one of the world's most economically important freshwater aquaculture species. Notably, grass carp reovirus (GCRV), classified as a double-stranded RNA (dsRNA) virus, has been identified as the causative pathogen of grass carp hemorrhagic disease (GCHD), which has resulted in substantial economic losses. This study aims to identify key genes involved in anti-GCRV innate immunity, with the ultimate goal of breeding anti-GCRV grass carp strains. In the present study, using zebrafish as a model, we demonstrated that <em>sirt7</em> deficiency enhances antiviral gene expression during GCRV infection. Subsequent cloning and analysis of the grass carp SIRT7 gene revealed its conservation across different species. The <em>Ci</em>-SIRT7 mRNA level was found to be highest in grass carp liver, and following GCRV infection, the <em>Ci</em>-SIRT7 mRNA level in the liver was kept stable. Conversely, the <em>Ci</em>-SIRT7 mRNA level was found to be comparatively low in grass carp intestine, and following GCRV infection, the <em>Ci</em>-SIRT7 mRNA level in the intestine was markedly induced. From a mechanistic perspective, <em>Ci</em>-SIRT7 has been observed to interact with <em>Ci</em>-IRF3 and <em>Ci</em>-IRF7, thereby inhibiting <em>Ci</em>-IRF3/7-mediated IFN activation. Furthermore, the results of this study demonstrated that <em>Ci</em>-SIRT7 promotes the ubiquitination and degradation of <em>Ci</em>-IRF3 and <em>Ci</em>-IRF7. In summary, the results presented here may contribute to the understanding of the role of grass carp SIRT7 in innate immunity against GCRV. In particular, SIRT7 is emerging as a promising target for developing novel grass carp strains resistant to GCRV infection.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742575"},"PeriodicalIF":3.9,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Essential role of RANK-NF-κB signaling pathway in spinal deformities: Insights from largemouth bass and zebrafish models","authors":"Zhuang Li ,&nbsp;Yajing Ouyang ,&nbsp;Xiaoyi Yuan ,&nbsp;Hongfei Zheng ,&nbsp;Jian Wang ,&nbsp;Chunxin Fan","doi":"10.1016/j.aquaculture.2025.742570","DOIUrl":"10.1016/j.aquaculture.2025.742570","url":null,"abstract":"<div><div>Spinal deformities, leading to reduced growth and survival rates, pose a significant challenge in fish aquaculture. However, the molecular mechanisms underlying their occurrence remain poorly understood. In this study, we investigated the role of the RANK-NF-κB signaling pathway in spinal deformities using both largemouth bass (<em>Micropterus salmoides</em>) and zebrafish (<em>Danio rerio</em>) models. We found that the expression of <em>nfkbiaa</em> and <em>nfkbiab</em> encoding NF-kappa-B inhibitor alpha and beta respectively was significantly upregulated in the deformed largemouth bass, indicating the involvement of NF-κB signaling in spinal deformities. Using the transgenic reporter zebrafish line <em>Tg(NFkB:EGFP)</em>, we observed strong NF-κB signaling activity in tissues surrounding the vertebral column. Inhibition of NF-κB signaling via overexpression of IkBSR (a dominant-negative inhibitor of NF-κB) resulted in ventral curvature of the spine in zebrafish which is similar to the phenotype of the deformed largemouth bass. Moreover, we generated <em>tnfrsf11a</em> mutant zebrafish using CRISPR-Cas9 and observed that these mutants exhibited ventral spinal curvature and a significant reduction in osteoclasts. Additionally, <em>tnfrsf11a</em> mutant zebrafish exhibited structural damage in lateral line canals, suggesting that the morphology of lateral line canals could serve as a non-invasive diagnostic marker for spinal deformities. For the first time, this study demonstrates that dysregulation of the RANK-NF-κB signaling pathway as a critical regulator of spinal deformities in fish, providing key insights into the molecular pathology of vertebral malformations and offering novel targets for prevention and therapeutic intervention.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742570"},"PeriodicalIF":3.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of a marine strain: Bacillus inaquosorum, and its feeding effects on Sebastes schlegelii as feed additive","authors":"Xin-Kun Zhu ,&nbsp;Ren-Ge Hu ,&nbsp;Wei Cong ,&nbsp;Yuan-Huan Kang ,&nbsp;Li-Yan Wang ,&nbsp;Tao Yang ,&nbsp;Shu Li","doi":"10.1016/j.aquaculture.2025.742556","DOIUrl":"10.1016/j.aquaculture.2025.742556","url":null,"abstract":"<div><div>The intensive development of industrial-scale aquaculture has led to overreliance on antimicrobial agents, raising global concerns about residual contamination and the proliferation of antimicrobial resistance. Marine-derived <em>Bacillus</em> spp., due to their proven probiotic effects, have emerged as a promising alternative to traditional antibiotics in aquaculture systems. A marine-derived <em>Bacillus inaquosorum</em> strain M1 demonstrating potent <em>Vibrio</em>-inhibitory activity was isolated from coastal sediment samples. Comprehensive assessments demonstrated that M1 exhibited excellent environmental adaptability and stability while maintaining sensitivity to commonly used antibiotics. To explore the potential applications of M1 in aquaculture, animal trials were conducted to evaluate its effects on <em>Sebastes schlegelii</em>, including growth performance, activity of digestive enzymes, intestinal health, and response of the immune system. The results revealed that oral M1 significantly enhanced the growth rate, feed efficiency, and intestinal digestive enzyme activities in <em>S. schlegelii</em> (<em>p</em> &lt; 0.05). Furthermore, improvements in intestinal structure and increased activities of immune and antioxidant enzymes were observed. Notably, levels of <em>IL-2</em> and <em>IFN-γ</em> in various organs of <em>S. schlegelii</em> were significantly elevated following dietary supplementation of M1 (<em>p</em> &lt; 0.05). Conversely, levels of <em>IL-1β</em>, <em>TNF-α</em>, and <em>IL-10</em> were reduced (<em>p</em> &lt; 0.05). Additionally, the fish exhibited enhanced resistance to <em>V. harveyi</em> infections, with a protection rate of up to 73.33 %. These findings suggested that <em>B. inaquosorum</em> M1 can modulate the immune function of <em>S. schlegelii</em>, thereby improving their growth performance and overall health status. Consequently, <em>B. inaquosorum</em> M1 is posited as a potential candidate for application as an aquaculture feed additive and a promising alternative to antibiotics.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742556"},"PeriodicalIF":3.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary bile acids reduced ferroptosis, improved the flesh quality and intestinal health of Pacific white shrimp (Litopenaeus vannamei) fed the high plant protein-based diets","authors":"Kangyuan Qu ,&nbsp;Junwei Zeng ,&nbsp;Junming Deng ,&nbsp;Xiaohui Dong ,&nbsp;Hongyu Liu ,&nbsp;Shuang Zhang ,&nbsp;Shuyan Chi ,&nbsp;Qihui Yang ,&nbsp;Beiping Tan ,&nbsp;Shiwei Xie","doi":"10.1016/j.aquaculture.2025.742562","DOIUrl":"10.1016/j.aquaculture.2025.742562","url":null,"abstract":"&lt;div&gt;&lt;div&gt;Bile acids (BAs) have been shown to improve the growth performance of shrimp and are essential for their lipid metabolism. This study examined the impacts of incorporating BAs into a diet rich in plant-based proteins on growth performance, flesh quality, intestinal health, and ferroptosis of &lt;em&gt;Litopenaeus vannamei.&lt;/em&gt; A total of seven diets were formulated, featuring a 25 % fish meal diet as the positive control (PC) and a 12.5 % fish meal diet designated as the negative control (NC). The remaining five diets were produced by supplementing varying amounts of BAs at 50, 100, 200, 400, and 800 mg/kg in the NC, labeled as NC50 to NC800. In comparison with the PC group, no differences were observed in the final body weight (FBW) and weight gain rate (WGR) of the NC group (&lt;em&gt;P&lt;/em&gt; &gt; 0.05). The FBW and WGR of the shrimp were significantly increased when 400 mg/kg BAs were added to the NC group (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). A comparison of shrimp from the PC and NC groups showed that the latter group had significantly higher crude lipid content (&lt;em&gt;P&lt;/em&gt; &lt; 0.05), which was further increased in shrimp fed the diets supplemented with more than 200 mg/kg BAs in the NC group (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). Immunofluorescence analysis indicated that the protein expression of GPX4 was lower in the NC group than in the PC and NC400 groups (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). Transmission electron microscopy analyses showed that the mitochondrial cristae disappeared, and the lipid peroxidation metabolite (MDA) content was significantly increased in the NC group compared to the PC group (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). Moreover, high-plant protein-based feed significantly increased the expression of genes linked to ferroptosis, including &lt;em&gt;acsl4&lt;/em&gt;, &lt;em&gt;hoi&lt;/em&gt;, and &lt;em&gt;fettrin&lt;/em&gt; (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). However, the inclusion of BAs can significantly diminish those mentioned metrics (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). Furthermore, the inclusion of BAs in high-plant protein diets not only decreased cooking loss but also enhanced the texture properties of shrimp muscle, including hardness, cohesiveness, resilience, and springiness (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). The supplementation of BAs also led to an upregulation of genes related to muscle fiber growth, specifically &lt;em&gt;tgf-β&lt;/em&gt;, &lt;em&gt;tor&lt;/em&gt;, &lt;em&gt;smych1&lt;/em&gt;, and &lt;em&gt;smych2&lt;/em&gt; (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). Results from the Western blot and histological analyses revealed a significant upregulation of myofibrillar proteins including Akt, 4E-BP1, Raptor, and MyoD1, which also led to a marked increase in the diameter of muscle fibers (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). Furthermore, electron microscopy revealed that incorporating BAs into high-plant protein diets led to a marked increase in both the width and height of the intestinal folds of shrimp, along with a growth in the density of microvilli (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). In conclusion, the inclusion of BAs into diets rich in plant-based proteins can enhance the growth performance, flesh quality, resi","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742562"},"PeriodicalIF":3.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"European eel larvae are sensitive to vibration and light","authors":"Fan Yang ,&nbsp;Elisa Benini ,&nbsp;Sune R. Sørensen ,&nbsp;Christoffer Moesgaard Albertsen ,&nbsp;Johanna Kottmann ,&nbsp;Ian A.E. Butts ,&nbsp;Jonna Tomkiewicz ,&nbsp;Sebastian N. Politis","doi":"10.1016/j.aquaculture.2025.742569","DOIUrl":"10.1016/j.aquaculture.2025.742569","url":null,"abstract":"<div><div>European eel (<em>Anguilla anguilla</em>) earliest life stages haven't been encountered in nature, thus, studying behavior of hatchery-reared larvae is crucial for understanding their ecology and developing rearing protocols. Utilizing computer-assisted video tracking we investigated the behavior of hatchery-reared eel larvae from hatching to the first-feeding stage at 12 days post hatch (dph). Trials focused on escape responses linked to mechanical (vibration) and visual (light) stimuli, repeated at 60 s intervals and selected to mirror stimuli encountered in hatchery settings. Variables included distance moved in single-frame intervals, distance moved in 120 ms (3 frames), initial escape speed, total distance moved in 5 s, mean and maximum speed for the entire response. Escape probabilities were estimated by the hidden Markov model. Results revealed that eel larvae's responses to stimuli were stage-specific, corresponding to the development of sensory systems, where the lowest and highest escape probabilities were observed at hatch and 12 dph, respectively. Larvae demonstrated fast reactions to mechanical stimuli (vibration), but a stronger sensitivity to sudden changes in light intensity, with average maximum escape response speeds up to 40 cm/s. Moderate but consistent escape responses to repeated vibrations, suggest a potential inherent ability to detect and respond to mechanical stimuli, while a decreasing escape response trend with repeated light exposures might indicate a degree of habituation potential to visual stimuli. In conclusion, we highlight the importance of tailoring rearing protocols for eel larvae, avoiding excessive disturbances, especially during highly sensitive stages, to reduce unnecessary stress for improved welfare in hatchery settings.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742569"},"PeriodicalIF":3.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroendocrine regulation of the metamorphic transition of giant clams","authors":"Keana Tan ,&nbsp;Danielle Mae Mateo-Matriano ,&nbsp;Sandie Degnan ,&nbsp;Cecilia Conaco","doi":"10.1016/j.aquaculture.2025.742563","DOIUrl":"10.1016/j.aquaculture.2025.742563","url":null,"abstract":"<div><div>For many benthic marine organisms, metamorphosis of larvae into juveniles is accompanied by a shift from the plankton into the benthos. This pelagobenthic transition must be precisely regulated and integrated with external environmental cues to allow the larva to settle at the right place, time, and conditions. Though the molecular mechanisms of the metamorphic processes in marine invertebrates remain relatively understudied, increasing evidence highlights the significant role played by neuroendocrine signaling in the modulation of this process. In this study, we examined the effect of the neurotransmitters gamma-aminobutyric acid, serotonin, epinephrine, and L-DOPA on the settlement and metamorphosis of the giant clam, <em>Hippopus hippopus</em>. We then determined the expression of genes encoding neurotransmitter-related enzymes and receptors during early development using transcriptomes of <em>H. hippopus</em> and a related species, <em>Tridacna gigas</em>. Epinephrine emerged as a promising candidate for inducing settlement and metamorphosis, while serotonin and L-DOPA may be involved in other settlement-related functions such as larval swimming and seeking behavior. This is supported by the upregulation of transcripts encoding enzymes involved in neurotransmitter biosynthesis during the competent pediveliger stage. The likely involvement of neurotransmitters in the regulation of the metamorphic process suggests their potential to boost spat yield in giant clam aquaculture. Beyond its applications in mariculture, these results provide insights into the complexity of the giant clam larval neuroendocrine system.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742563"},"PeriodicalIF":3.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}