Aquaculture最新文献

{"title":"Frequent horizontal transfer of AHPND-related plasmid among Vibrionaceae in the rotifers and its implication for shrimp disease management","authors":"Songzhe Fu ,&nbsp;Ziqiang Zhang ,&nbsp;Wenyu Zhao ,&nbsp;Rui Wang ,&nbsp;Junfeng Hui ,&nbsp;Qian Yang","doi":"10.1016/j.aquaculture.2025.742446","DOIUrl":"10.1016/j.aquaculture.2025.742446","url":null,"abstract":"<div><div>Acute hepatopancreatic necrosis disease (AHPND) caused by <em>Vibrio parahaemolyticus</em> is a newly emerging bacterial disease in the cultured shrimp. However, it remains unclear how AHPND-related plasmid can be disseminated among bacterial species via live feed. To this end, we first developed an epicPCR method to amplify the fusion products of <em>pirB</em>-16S rRNA, which was then used to confirm the host range of the AHPND-related plasmid. Next, we traced the horizontal transfer of <em>pirB</em> by inoculating rotifers with <em>pirB</em>-positive <em>V. parahaemolyticus</em> into three shrimp tanks and maintained the cultivation of shrimp from stage N5 to the post-larval stage PL18. Vibrionaceae were isolated from the rearing water and rotifers at day 36. Meanwhile, shotgun metagenomic sequencing (SMS) was performed for rearing water and rotifers to understand the dynamics of microbial community. Results showed that AHPND-related plasmid not only disseminated among <em>Vibrio</em> spp., but also transferred into other genera such as <em>Shewanella</em>, <em>Aeromonas</em> and <em>Photobacterium</em> in the rearing water and rotifers. SMS showed that relative to the rearing water, higher abundance of <em>Vibrio</em> spp. was found in rotifers. Subsequently, we also isolated <em>Vibrio</em> spp., <em>Shewanella</em>, and <em>Photobacterium</em> from the rearing water and rotifers, confirming the presence of AHPND-related plasmid in above genera. Moreover, rotifers harbored more strains carrying the AHPND-related plasmid, suggesting that the rotifers acted as potential vectors for its dissemination. These results underscored the need to include live feeds in the assessment of potential biosecurity risks.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742446"},"PeriodicalIF":3.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miRNA-mRNA interaction mechanism in echinoderm Apostichopus japonicus in response to acute hypoxia","authors":"Yanlin Wang ,&nbsp;Yingqiu Zheng ,&nbsp;Yingchao Yin ,&nbsp;Lidong Sun ,&nbsp;Muyan Chen","doi":"10.1016/j.aquaculture.2025.742487","DOIUrl":"10.1016/j.aquaculture.2025.742487","url":null,"abstract":"<div><div>The expanding low-oxygen areas in marine environments due to global climate change have posed a significant challenge to the survival of marine organisms and the health of ecosystems. The echinoderm <em>Apostichopus japonicus</em>, a sea cucumber species essential in benthic communities, has been experiencing annual mass mortality in summer in recent years due to hypoxia and other extreme events. Here, we observed sea cucumber behavioral plasticity and mortality in response to acute hypoxia, which was investigated at the molecular level using RNA-seq and miRNA-seq of coelomocytes to determine transcriptional and post-transcriptional regulation. The results indicated that sea cucumbers extended their tentacles and elongated their bodies to acquire more oxygen from the water. The enrichment analysis of differentially expressed genes (DEGs) suggested that “Ribosome biogenesis in eukaryotes” pathway was significantly downregulated and GO item “phagocytosis” was significantly upregulated, indicating that energy consumption reduction and immune activities increase are key response strategies to tolerance to hypoxia stress. Then, we predicted the potential miRNA-mRNA regulatory network involved in both processes and identified the evolutionarily conserved miRNAs that may be responsible for hypoxia stress in marine invertebrates. Our study provides insights into the behavioral plasticity and regulatory mechanism of sea cucumbers immune system in response to limited oxygen conditions and offers implications for the development of strategies used for aquaculture and wild population protection in the context of global climate change.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742487"},"PeriodicalIF":3.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Survival and toxin accumulation in Crassostrea virginica larvae following exposure to various Dinophysis species” [Aquaculture, Volume 602, 30 May 2025, 742294]","authors":"Nour Ayache ,&nbsp;Marta Sanderson ,&nbsp;Jessica Small ,&nbsp;Amanda B. Chesler-Poole ,&nbsp;Sabrina D. Giddings ,&nbsp;Lisa Campbell ,&nbsp;Vera L. Trainer ,&nbsp;Michael L. Brosnahan ,&nbsp;Juliette L. Smith","doi":"10.1016/j.aquaculture.2025.742462","DOIUrl":"10.1016/j.aquaculture.2025.742462","url":null,"abstract":"","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742462"},"PeriodicalIF":3.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different feeding modes on the growth, reproduction, digestion, stress tolerance, and intestinal microflora of pre-adult red swamp crayfish Procambarus clarkii","authors":"Yu Ding ,&nbsp;Yunfei Sun ,&nbsp;Yongxu Cheng","doi":"10.1016/j.aquaculture.2025.742479","DOIUrl":"10.1016/j.aquaculture.2025.742479","url":null,"abstract":"<div><div>In this study, we sought to establish a suitable feeding mode for the aquaculture of pre-adult red swamp crayfish (<em>Procambarus clarkii</em>). To this end, we assessed the effects of feeding crayfish with a traditional feed (TF), chilled fish (CF), or formulated diet (FD)on growth and reproductive performance, digestion and stress tolerance, and the composition of the intestinal microflora. The results revealed that TF-fed crayfish had the best growth performance and digestive system, whereas CF-fed crayfish had the highest reproductive performance and microbial community α-diversity, and FD-fed crayfish had the strongest stress tolerance. With respect to the intestinal microflora of crayfish, we detected similar microfloral compositions among the different treatment groups, with <em>Bacteroidota</em>, <em>Firmicutes</em>, <em>Proteobacteria</em>, and <em>Actinobacteriota</em> being identified as the predominant phyla, and <em>Bacteroides</em>, <em>Citrobacter</em>, <em>RsaHf231</em>, <em>Candidatus_Bacilloplasma</em>, <em>Lactococcus</em>, <em>Tyzzerella</em>, <em>Gordonia</em>, and <em>ZOR0006</em> predominating at the genus level. However, at the phylum level, there were significant differences among groups with respect to the relative abundances of <em>Bacteroidota</em> and <em>Actinobacteriota</em>, and at the genus level, there were differences in the relative abundance of <em>Gordonia</em> and <em>Tyzzerella.</em> Collectively, our findings indicate that the provision of traditional and formulated diets contribute to balanced growth performance, digestion, and stress tolerance, whereas a diet of chilled fish is conducive to obtaining optimal reproductive performance and microflora α-diversity. However, the mode of feeding appeared to have only limited effects on microfloral community composition in pre-adult crayfish. These findings will provide a theoretical reference for bait selection and the aquacultural production of pre-adult crayfish.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742479"},"PeriodicalIF":3.9,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of an indirect ELISA procedure for detecting Aeromonas infections on the basis of flagellar cap protein FliD antigen in Cyprinus carpio","authors":"Zhao Cheng,&nbsp;Chao Pei,&nbsp;Lei Zhu,&nbsp;Chen Li,&nbsp;Xianghui Kong","doi":"10.1016/j.aquaculture.2025.742481","DOIUrl":"10.1016/j.aquaculture.2025.742481","url":null,"abstract":"<div><div><em>Cyprinus carpio</em> is a vital part fisheries resource breed and one of the world's most valuable commercial fish. <em>Aeromonas</em> is a very important aquatic pathogen, which causes high mortality and great financial losses in aquaculture. It is necessary to establish a rapid, specific and sensitive diagnostic method of immunoglobulin M (IgM) antibodies for the detection of <em>Aeromonas</em> infection in <em>C. carpio</em>. In this study, the recombinant FliD protein was utilized as the coated antigen to develop a novel indirect ELISA (enzyme-linked immunosorbent assay) for the detection of specific anti-<em>Aeromonas</em> IgM antibodies in <em>C. carpio</em>. The established FliD-ELISA showed high specificity and sensitivity, and according to the cut-off value, it can detect the anti-FliD antibody at a maximal dilution of 1:800 in <em>C. carpio</em> serum The coefficient of variation (CV) of the FliD-ELISA was less than 10 % for both intra- and inter-assay, which meets the requirements. Compared with the clinical serum samples, the established FliD-ELISA and commercial Fish pathogenic <em>Aeromonas</em> ELISA kit displayed a similar compliance rate of 95.24 %. The findings of the experiments show that the newly developed FliD-ELISA is a convenient and reliable method for detecting anti-<em>Aeromonas</em> antibodies, and it could monitor the epidemiology of <em>Aeromonas</em> infection in <em>C. carpio</em> in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742481"},"PeriodicalIF":3.9,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143792564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Carica papaya and alpha-lipoic acid synergistically promote resistance to Aeromonas hydrophila, digestive function, growth, physiological, and antioxidant responses of Nile tilapia (Oreochromis niloticus) raised under high densities","authors":"Mohamed F. Abdel-Aziz ,&nbsp;Afaf N. Abdel Rahman ,&nbsp;Ashraf Y. El-Dakar ,&nbsp;Khalil Mahmoud Khalil ,&nbsp;Mahmoud Mahrous M. Abbas ,&nbsp;Randa Yousry Thabet","doi":"10.1016/j.aquaculture.2025.742478","DOIUrl":"10.1016/j.aquaculture.2025.742478","url":null,"abstract":"<div><div>Feed additives are edible ingredients that are very sparingly added to fish diets to improve the nutritional value of feed, which in turn enhances fish growth and health. Most recent research focused on their single use, but little is known about their combined application. Hence, a 7-week feeding trial was conducted to investigate the consequences of the dietary addition of papaya (<em>Carica papaya</em>) leaf powder (CPP) with or without alpha-lipoic acid (AL). The assessment encompassed the influence on growth, digestive capacity, body composition, physiological and antioxidant response, as well as disease resistance (<em>Aeromonas hydrophila</em> challenge). Five treatments containing 225 fish (<em>N</em> = 45 fish/group, 3 polyethylene cages /group, 15 fish/cage) with an average weight of 6.65 ± 1.85 g were haphazardly assigned. For 7 weeks, fish received basal diet supplemented with CPP alone or with AL [0 % (control), 2 %CPP (CPP2), 3 %CPP (CPP3), 2 % CPP with 0.1 %AL (CPP2 + AL0.1), and 3 %CPP with 0.1 %AL (CPP3 + AL0.1)]. Results displayed that CPP alone or with AL-fortified diets substantially boosted growth performance and digestive enzyme activity (amylase, lipase, and trypsin). Dietary CPP alone or with AL notably increased body lipid content, serum total protein, and hepatic antioxidant (reduced glutathione, superoxide dismutase, and total antioxidant capacity) parameters. Moreover, blood glucose and hepatic malondialdehyde showed lower values in the CPP alone or with AL diets. Surprisingly, the combination groups especially the CPP2 + AL0.1 group achieved the best results (<em>P</em> &lt; 0.05) for these variables. Fifteen days post-challenge with <em>A. hydrophila</em>, the cumulative mortality declined among fish fed CPP alone or with AL diets, where the CPP2 + AL0.1 group did not record any mortality. Overall, the dietary intervention of CPP (2 %) with LA (0.1 %) by their synergistic potential can promote disease resistance, physiological-antioxidant status, growth, and digestive capacity of Nile tilapia. These advantageous outcomes can support the development of sustainable aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742478"},"PeriodicalIF":3.9,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of fruitless1 isoforms and their molecular function in gonadal development and courtship in the mud crab Scylla paramamosain","authors":"Mengqian Zhang ,&nbsp;Yongcheng Xue ,&nbsp;Bixun Qiu ,&nbsp;Shihao Shi ,&nbsp;Xiyi Zhou ,&nbsp;Yixi Chen ,&nbsp;Hai Liu ,&nbsp;Wenfeng Han ,&nbsp;Shaharior Hossen ,&nbsp;Hongyu Ma","doi":"10.1016/j.aquaculture.2025.742467","DOIUrl":"10.1016/j.aquaculture.2025.742467","url":null,"abstract":"<div><div>The <em>fruitless</em> (<em>fru</em>) gene is a key regulator of courtship behavior and the sex determination pathway in crustaceans. The present study identified two isoforms of <em>fru1</em>, <em>Sp-fru1a</em> and <em>Sp-fru1b</em> in the mud crab <em>Scylla paramamosain</em>. Sequence analysis revealed conserved BTB and C2H2-ZnF domains in their deduced amino acid sequences. Genomic analysis suggested that both isoforms originated from a single genomic locus. Fluorescence <em>in situ</em> hybridization confirmed the co-localization of both isoforms in the cytoplasm of oocytes and spermatids of the gonad. <em>Sp-fru1</em> showed sexually dimorphic expression in well-developed gonads, with <em>Sp-fru1a</em> mRNA highly expressed in ovaries and <em>Sp-fru1b</em> mRNA predominantly in testis. <em>Sp-fru1a</em> mRNA expression remained high and consistent at various stages, with no significant changes observed, while <em>Sp-fru1b</em> mRNA expression was increased from unmated to the mating stage, and after mating, the expression level overall showed a downward trend. Moreover, both isoforms showed significantly higher expression in pre-mated testis. RNAi-mediated knockdown of <em>Sp-fru1a</em> resulted in lower mRNA expression of <em>vasa</em>, <em>piwi2</em>, and <em>Vg</em> in the ovary, leading to incomplete and fragmented egg cells. Similarly, knockdown of <em>Sp-fru1b</em> resulted in lower mRNA expression of <em>vasa</em>, <em>piwi2</em>, and <em>dmrt1</em> in the testis, leading to a reduction in spermatozoa within the seminiferous tubules. Present findings suggest that <em>Sp-fru1</em> plays key roles in sex-specific development and courtship behavior, providing valuable insights into the sex determination mechanisms and reproductive biology in crustaceans. This study emphasizes the significance of <em>Sp-fru1</em> in the reproductive physiology of <em>S. paramamosain</em>, offering potential applications for molecular breeding, monosex population production, unisex farming, and aquaculture management through the control of unintended reproduction.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742467"},"PeriodicalIF":3.9,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo assessment of Nelumbo nucifera, Glycines testa, and Agrimonia eupatoria extracts against different developmental stages of Ichthyophthirius multifiliis","authors":"Youzhi Meng ,&nbsp;Hao Yang ,&nbsp;Xiao Tu ,&nbsp;Zemao Gu","doi":"10.1016/j.aquaculture.2025.742465","DOIUrl":"10.1016/j.aquaculture.2025.742465","url":null,"abstract":"<div><div>The ciliate <em>Ichthyophthirius multifiiis</em> is one of the most pathogenic parasites of freshwater fish, has three life cycle stages. The use of natural plants for prevention and treatment of ichthyophthiriasis has a good prospect. In the present study, <em>Nelumbo nucifera, Glycines testa, and Agrimonia eupatoria</em> ethanolic extracts were selected among 70 extracts through screening experiment targeting theronts. Then, we evaluated <em>in vitro</em> parasiticidal effects of the three extracts against three life stages of <em>I. multifiiis</em>. The EC₅₀ values of <em>N. nucifera, G. testa</em>, and <em>A. eupatoria</em> extracts against theronts were 2.18, 3.63, 2.63 mg/L, respectively, and the corresponding EC₅₀ values against protomonts were 11.02, 10.02, 10.08 mg/L, and against tomonts were 34.68, 21.48, 32.50 mg/L. To achieve a comprehensive assessment of these three extracts, we investigated their therapeutic effect <em>in vivo</em> and the toxicity to goldfish (<em>Carassius auratus</em>). After continuous immersion at the maximum nonlethal concentration (50, 30, 50 mg/L) of <em>N. nucifera</em>, <em>G. testa</em>, and <em>A. eupatoria</em> extracts for 17 days, the infection intensity of caudal fin was reduced by 90.98 %, 91.95 %, 85.08 % compared to the control group, and the survival rate of infected goldfish was increased by 13.33 %, 46.67 %, 66.67 %. The 96 h acute toxicity test to goldfish showed that the LC₅₀ values of <em>N. nucifera, G. testa</em>, and <em>A. eupatoria</em> extracts were 95.16, 53.42, 90.76 mg/L, respectively, demonstrating three extracts were safe to host. Collectively, our findings highlight <em>A. eupatoria</em> extract has the promising application prospects in prevention and control of ichthyophthiriasis.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742465"},"PeriodicalIF":3.9,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient antiviral activity of a novel magnolol derivative against aquatic rhabdoviruses","authors":"Fei Yang ,&nbsp;Zhiqiang Zhang ,&nbsp;Shenye Qu ,&nbsp;Gaoxue Wang ,&nbsp;Qing Yu ,&nbsp;Fei Ling","doi":"10.1016/j.aquaculture.2025.742464","DOIUrl":"10.1016/j.aquaculture.2025.742464","url":null,"abstract":"<div><div><em>Micropterus salmoides</em> rhabdovirus (MSRV) is a prominent pathogen that mainly endangers largemouth bass fry, which often causes devastating diseases to largemouth bass and leads to huge economic losses in aquaculture. So far, there is no approved effective measure to control MSRV infection. In the study, 24 magnolol derivatives were used to conduct anti-MSRV activity screening by RT-qPCR, and the results indicated that 5,5′-diallyl-3,3′-dinitro-[1,1′-biphenyl]-2,2′-diol (M3) exhibited the best anti-MSRV efficiency with IC₅₀ of 0.92 μM. Moreover, further study showed that the CPE induced by MSRV was obviously weaken by M3, and the viral titers were also significantly decreased by M3 within 96 h after MSRV infection (<em>p</em> &lt; 0.05). Next, the anti-MSRV mechanism of M3 was preliminarily investigated, and the findings illustrated that M3 did not directly act on MSRV virion which had no effect on the infectivity of MSRV. Furthermore, the adsorption of MSRV onto GCO cells was not hindered by M3. The results of cell cycle detected by flow cytometry demonstrated that M3 altered the state of cell cycle arrest in S phase induced by MSRV, influencing the environment required for virus replication. In addition, observation of survival rate and determination of viral load were conducted to further verify the antiviral activity of M3 against MSRV. The results illustrated that administration with M3 at the dose of 20 mg/kg <em>via</em> intraperitoneal injection significantly enhanced the survival rate of largemouth bass infected with MSRV by 35.98 % (<em>p</em> &lt; 0.01). Moreover, the viral loads of liver, spleen and kidney were significantly decreased by M3 on day 3 and day 5 post MSRV infection (<em>p</em> &lt; 0.05). Finally, the antiviral effects of M3 against aquatic rhabdoviruses spring viremia of carp virus (SVCV) and infectious hematopoietic necrosis virus (IHNV) were explored, and the results demonstrated that M3 evidently lessened the production of CPE induced by SVCV and IHNV, and inhibited the proliferation of SVCV and IHNV in EPC cells in a dose-dependent manner, with IC₅₀ of 2.92 μM and 2.78 μM, respectively. To sum up, M3 exhibits a high antiviral activity against MSRV, and is expected to be developed into antiviral specific drugs against rhabdoviruses in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742464"},"PeriodicalIF":3.9,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics-driven lipidomics reveal the hepatic nutrition and lipid metabolism during ovarian development of Conger myriaster","authors":"Guixiang Wang ,&nbsp;Xiangbiao Zeng ,&nbsp;Kang Li ,&nbsp;Rucong Liu ,&nbsp;Zhixin Jiang ,&nbsp;Xubing Ba ,&nbsp;Zhenzhu Wei ,&nbsp;Jingwei Liu ,&nbsp;Liping Liu","doi":"10.1016/j.aquaculture.2025.742460","DOIUrl":"10.1016/j.aquaculture.2025.742460","url":null,"abstract":"<div><div>Nutrient and energy metabolism are crucial prerequisites for sexual maturation of eels. However, studies on the mechanisms of nutrient and energy metabolism in the liver during ovarian maturation in female <em>Conger myriaster</em> are limited, hindering successful artificial breeding. This study conducted transcriptomic and lipidomics sequencing on liver tissues from different stages of ovarian development (I, IV, VIII) to explore important nutritional regulatory and energy mobilization factors and pathways. We observed that the activities of fatty acid synthase, hepatic lipase, and lipoprotein lipase increased with developmental stages, while the activities of aspartate transaminase and total protein decreased. Functional annotation indicated that 2731 differentially expressed genes (DEGs) in stage IV vs stage VIII were associated with processes such as steroid hormone biosynthesis, steroid biosynthesis, linoleic acid metabolism, fat digestion and absorption, and vitamin digestion and absorption. Lipidomics analysis (67 differential lipid metabolites) revealed that energy redistribution in the liver during gonadal development was primarily regulated through glycerophospholipid metabolism, linoleic acid metabolism, and GPI biosynthesis pathways, which were responsible for the catabolism of stored energy reserves. Additionally, we identified several key metabolites and genes involved in the metabolism of phospholipids, glycerolipids, amino acids, and vitamins, contributing to the assembly and transport of essential nutrients in the liver. This study provided new insights into energy utilization during ovarian development in <em>C. myriaster</em> and offered critical information for optimizing nutritional strategies in artificial breeding.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742460"},"PeriodicalIF":3.9,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}