Aquaculture最新文献

{"title":"Effects of varying levels of dietary crude fibre on growth, physio-biochemical responses, and gut microbiota in Pacific white shrimp, Penaeus vannamei","authors":"S.R. Gokulnath ,&nbsp;J. Syama Dayal ,&nbsp;Tincy Varghese ,&nbsp;R. Ananda Raja ,&nbsp;T. Sivaramakrishnan ,&nbsp;K. Ambasankar ,&nbsp;Ashutosh D. Deo ,&nbsp;N. Ranjith ,&nbsp;N. Suganya ,&nbsp;J. Kumaravel ,&nbsp;J. Ashok Kumar","doi":"10.1016/j.aquaculture.2025.743157","DOIUrl":"10.1016/j.aquaculture.2025.743157","url":null,"abstract":"<div><div>Crude fibre (CF) plays a crucial role in modulating gut health and nutrient utilisation in aquaculture species. A 45-day feeding trial was conducted to evaluate the effects of dietary CF on growth performance, nutrient utilisation, digestion, hepatopancreas and intestinal histology, antioxidant enzyme activity, and intestinal microbiota of Pacific white shrimp, <em>Penaeus vannamei</em>. Five isonitrogenous and isolipidic diets, namely F2.58, F5.20, F7.62, F10.20, and F12.56, were formulated with varying CF levels (2.58, 5.20, 7.62, 10.20, and 12.56 %), using cellulose as the fibre source. Growth parameters, including weight gain (WG), weight gain percentage (WGP), specific growth rate (SGR), feed conversion ratio (FCR), and protein efficiency ratio (PER), varied significantly among treatments (<em>P</em> &lt; 0.05), with higher CF levels (7.62, 10.20, and 12.56 %) reducing growth and increasing FCR. Orthogonal contrast analysis revealed significant linear effects (<em>P</em> &lt; 0.001) for WG, WGP, SGR, FCR, and PER, while quadratic effects were not significant (<em>P</em> &gt; 0.05). However, survival rate, hepatosomatic index (HSI), and whole-body chemical composition remained unaffected (<em>P</em> &gt; 0.05). An increase in CF levels above 5.20 % significantly reduced digestive and antioxidant enzyme activities (<em>P</em> &lt; 0.05), indicating impaired digestion and increased oxidative stress. Histological analysis revealed severe structural damage in the hepatopancreas and midgut at higher CF levels, including intertubular oedema, cell hypertrophy, and epithelial degradation. Intestinal microbiota analysis indicated distinct microbial compositions among treatments, with moderate CF levels (5.20 %) promoting beneficial genera like <em>Meridianimaribacter</em> and suppressing potential pathogens. These findings suggest that an optimal CF level of up to 5.20 % maintains growth, digestive function, and gut microbial balance, whereas excessive CF compromises shrimp health and performance. This study provides valuable insights into the role of dietary fibre in shrimp nutrition and underlines the importance of dietary fibre optimisation for sustainable aquaculture practices.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743157"},"PeriodicalIF":3.9,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of water temperature on the depletion of enrofloxacin and its metabolite ciprofloxacin in largemouth bass (Micropterus salmoides) tissue following repeated oral administrations","authors":"Ruiqi Fan ,&nbsp;Xinyue Wang ,&nbsp;Yuanyuan Ren ,&nbsp;Shuang Li ,&nbsp;Wenfei Guo ,&nbsp;Jiaqi Liu ,&nbsp;Yingchun Mu ,&nbsp;Xiaoyu Wang ,&nbsp;Sudong Xia ,&nbsp;Bo Cheng","doi":"10.1016/j.aquaculture.2025.743154","DOIUrl":"10.1016/j.aquaculture.2025.743154","url":null,"abstract":"<div><div>Enrofloxacin (ENR) is a veterinary antibiotic commonly used in aquaculture in some regions. However, currently established withdrawal periods do not adequately consider the varying residue depletion characteristics of ENR across different cultured species and under different temperature conditions. This study investigated temperature-dependent depletion of ENR and its metabolite ciprofloxacin (CIP) in largemouth bass at 17 °C, 22 °C, and 27 °C. Fish received oral ENR (20 mg/kg·bw) for five consecutive days, with tissue samples collected from 1 to 48 days post-administration and analyzed by HPLC-MS/MS. Results demonstrated significant temperature-dependent elimination kinetics, with higher temperatures accelerating residue depletion in all tissues. At 17 °C, ENR half-lives ranged from 2.72 days (liver) to 4.78 days (plasma), while at 27 °C, these decreased to 2.27 days and 4.30 days, respectively. The metabolite CIP exhibited faster overall depletion than the parent compound, with greater temperature sensitivity particularly in plasma and liver tissues. Tissue-specific depletion patterns revealed liver as a site of rapid clearance, followed by skin-on muscle, while kidney and gill tissues demonstrated slower depletion rates. Temperature significantly influenced withdrawal periods, with calculated withdrawal times for ENR and CIP in skin-on muscle decreasing from 29.57 days at 17 °C to 23.90 days at 27 °C. This study highlights the critical importance of incorporating temperature considerations into ENR withdrawal period determination, as current fixed withdrawal recommendations may be inadequate for ensuring food safety across varying environmental conditions.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743154"},"PeriodicalIF":3.9,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosome-scale genome assemblies and population re-sequence of the mango tilapia provide insights into the adaptive evolution","authors":"Lingyu Kong ,&nbsp;Liming Luo ,&nbsp;Hengquan Zhao ,&nbsp;Kun Zhou ,&nbsp;Yan Zhou ,&nbsp;Weiqi Jiang ,&nbsp;Yusen Li ,&nbsp;Zhichang He ,&nbsp;Yongju Luo ,&nbsp;Liting Chen ,&nbsp;Ying Lu","doi":"10.1016/j.aquaculture.2025.743156","DOIUrl":"10.1016/j.aquaculture.2025.743156","url":null,"abstract":"<div><div>Mango tilapia (<em>Sarotherodon galilaeus</em>) is a commercially important species valued for its ecological resilience and nutritional profile. To elucidate its genome structure and environmental adaptability, we sequenced a male adult by combined technologies of Illumina sequencing, PacBio sequencing, and high-throughput chromosome conformation capture (Hi-C). A 0.98 Gb chromosome-scale assembly of mango tilapia (GenBank accession No. GCA_046562855.1) were constructed with a contig N50 length of 31.06 Mb, which was fully annotated to predict 31,865 protein-coding and 4448 non-coding genes. The BUSCO assessment of genome and annotation respectively exhibited a 97.97 % and 97.25 % completeness. A reconstructed phylogenetic tree using 2763 one-to-one single-copy genes estimated that mango tilapia diverged from its closest related species Nile tilapia 15.95 million years ago. Comparative analyses across 12 <em>Actinopterygii</em> observed that it underwent the frequent large-scale fragment duplications, resulting in a rapidly expansion of the gene families involved in environmental responses. A genome re-sequencing of three wild populations detected a total of 7,201,267 SNPs among the populations, through which a selection sweep analysis of a population in the north of the Tropic of Cancer against another two populations in the south of that localized 49 candidate genes under the significant selection. The 49 genes were annotated to be mainly involved in cell signaling and environmental responses, of which a homolog encoding DNA (cytosine-5)-methyltransferase 1 (DNMT1), a key enzyme responsible for maintaining DNA methylation in environmental stress responses, carried 13 homozygous substitutions only in the CW population, including a missense substitution in its coding region. These findings suggested a rapid evolution of adaption to the changes in environmental temperature. A fully annotated genome of mango tilapia was supposed to benefit for molecular breeding of tilapias.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743156"},"PeriodicalIF":3.9,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variation in individual feed consumption rates of intensively reared pikeperch (Sander lucioperca L. 1758) under feeding hierarchy","authors":"Tamás Molnár ,&nbsp;Gábor Beliczky ,&nbsp;András Bognár ,&nbsp;Béla Urbányi ,&nbsp;Ildikó Benedek","doi":"10.1016/j.aquaculture.2025.743119","DOIUrl":"10.1016/j.aquaculture.2025.743119","url":null,"abstract":"<div><div>The final stage of pikeperch (<em>Sander lucioperca</em> L. 1758) domestication, which includes the implementation of selective breeding programs, has only recently started. Beyond growth rate, feed intake and efficiency are also significant economic aspects of the species during selective breeding. Quantifying them on an individual level is challenging, and it is further complicated by the biasing effect of the feeding hierarchy. Our results demonstrate that measuring the individual feed intake over seven consecutive days already achieves a correlation of 0.95 with total intake over the entire period. However, 14 days are required for ICC-based repeatability of the trait to reach 0.95. In our study, we observed 23 feeding hierarchies in 13 of the 18 tanks, resulting in 25 % dominants, 22 % subordinates, and 53 % intermediate individuals for a total of 72 pikeperch fingerlings. The feeding hierarchy influenced all production parameters (feed intake, body weight gain, SGR, FCR) except RFI and survival rate. This resulted in lower and more fluctuating daily feed intake and poorer growth, but similar feed conversion in the subordinate individuals. Since RFI was not influenced by hierarchy formation, it may prove to be a good metric for implementing a selection program for feed efficiency.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743119"},"PeriodicalIF":3.9,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144989335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide DNA methylation profiling reveals epigenetic regulation of sex differentiation and gonadal development in Apostichopus japonicus","authors":"Yixin Wang,&nbsp;Xuemin Wang,&nbsp;Yanlin Wang,&nbsp;Muyan Chen","doi":"10.1016/j.aquaculture.2025.743153","DOIUrl":"10.1016/j.aquaculture.2025.743153","url":null,"abstract":"<div><div>The sea cucumber (<em>Apostichopus japonicus</em>) is an economically important marine benthic echinoderm. Understanding the molecular mechanisms of sex determination and differentiation is critical for improving sex-controlled breeding strategies. Epigenetic modifications serve as a crucial link between an organism's genetic information and environmental cues, playing a fundamental role in determining sex fate and conferring sexual plasticity across various species. The echinoderms exhibit an extremely diverse reproductive strategies, including both sexual reproduction and asexual reproduction. However, the molecular mechanisms governing epigenetic regulation in the sex differentiation process within echinoderms remain elusive. Here, we integrated genome-wide DNA methylation profiling and transcriptomic analysis of mature male and female gonads to investigate epigenetic regulation during sex differentiation. We identified 12,605 differentially methylated regions (DMRs) and 2764 differentially methylated genes (DMGs). By comparing the methylation levels of genes with different expression patterns, non-specific, or housekeeping genes, show high methylation in gene body region, while specifically expressed genes have lower methylation, which suggested that CpG methylation targeting housekeeping genes preferentially. Protein-protein interaction networks and functional enrichment analysis of differentially expressed methylated genes (DEMGs) revealed significant associations with sex-related processes, including steroid metabolism (<em>hsd17β4</em>, <em>cyp17a1</em>), meiosis (<em>dmc1</em>, <em>mei4</em>, <em>msh4</em>), spermatogenesis (<em>cfap</em>, <em>kif</em> families), and oogenesis (<em>fmn2</em>, <em>igf2bp3</em>). Our findings demonstrate that DNA methylation dynamically regulates transcriptional programs governing sex differentiation in <em>A. japonicus</em>, providing a molecular basis for enhancing reproductive management in sea cucumber aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743153"},"PeriodicalIF":3.9,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine vision-based %PP trait drives GWAS of inner shell color in Hyriopsis cumingii","authors":"Xuefeng Lv ,&nbsp;Quanzhi Bao ,&nbsp;Zhiyan Wang ,&nbsp;Yuyang Wu ,&nbsp;Renwei Wang ,&nbsp;Jiale Li ,&nbsp;Zhiyi Bai","doi":"10.1016/j.aquaculture.2025.743155","DOIUrl":"10.1016/j.aquaculture.2025.743155","url":null,"abstract":"<div><div>Inner shell color, particularly purple nacre, is a key determinant of pearl quality in <em>Hyriopsis cumingii</em>, a cornerstone of China's freshwater pearl industry. We conducted a genome-wide association study (GWAS) on 590 F4-generation individuals using custom liquid SNP arrays: a 30 K array targeting 30,000 SNPs and a 95 K array that includes these 30 K SNPs plus additional loci. After stringent quality control, 25,900 (30 <em>K</em>) and 82,585 (95 K) SNPs were analyzed, identifying significant associations with seven inner shell color traits—L*, a*, b*, dE, C*, h*, and a novel machine vision-derived purple pixel percentage (%PP)—on chromosomes 17 and 15. The %PP trait enhances GWAS precision by quantifying purple nacre coverage. Notably, the 95 K array and 30 K array showed partial SNP overlap, with significant loci under <span><math><msub><mrow><mo>−</mo><mi>log</mi></mrow><mn>10</mn></msub><mfenced><mrow><mn>0.05</mn><mo>/</mo><mi>N</mi></mrow></mfenced></math></span> and <span><math><msub><mrow><mo>−</mo><mi>log</mi></mrow><mn>10</mn></msub><mfenced><mrow><mn>1</mn><mo>/</mo><mi>N</mi></mrow></mfenced></math></span> thresholds forming congruent and divergent sets, reflecting the interplay between SNP density and threshold settings. Key SNPs, including LG17_17440155 and LG17_17816229, were validated in 130 independent <em>H. cumingii</em> individuals, confirming their associations with L*, a*, C*, and dE for LG17_17440155, and with L*, a*, C*, and %PP for LG17_17816229. Candidate genes, including <em>pksM</em>, <em>pks1</em>, and SLC family members (<em>SLC13A2_3_5</em>, <em>SLC6A8</em>, etc.), were validated in an independent F2 population (<em>n</em> = 463), implicating pigment synthesis and transport pathways; validation of the combined significant SNPs from both arrays further confirmed their reliability, with <span><math><msub><mrow><mo>−</mo><mi>log</mi></mrow><mn>10</mn></msub><mfenced><mrow><mn>0.05</mn><mo>/</mo><mi>N</mi></mrow></mfenced></math></span> demonstrating higher credibility and reduced false positives. These findings provide genetic markers for selective breeding and deepen insights into molluscan pigmentation genetics.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743155"},"PeriodicalIF":3.9,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of high-efficiency spawning methods and gonadal stress responses in tropical sea cucumber Holothuria leucospilota","authors":"Yikun Cen ,&nbsp;Yingqiu Zheng ,&nbsp;Dejian Su ,&nbsp;Dong Wang ,&nbsp;Muyan Chen ,&nbsp;Zonghe Yu","doi":"10.1016/j.aquaculture.2025.743152","DOIUrl":"10.1016/j.aquaculture.2025.743152","url":null,"abstract":"<div><div><em>Holothuria leucospilota</em>, a commercially significant sea cucumber species, demands improved aquaculture methods for sustainable production. Here, we present a dual breakthrough in spawning induction methodology and the mechanistic understanding of reproductive regulation. First, we developed a novel induction method combining low temperature air-desiccation (16 °C, 30 min) with chemical hypoxia (0.1 g L⁻¹ Na₂SO₃, 30 min), achieving 70–100 % spawning success (5.0–6.7 million eggs female⁻¹) within 1–2 h post-stimulus in this species, using spawning induction rate and total/mean egg output as evaluation indicators. Multi-omics integration revealed sex-specific regulatory mechanisms in <em>H. leucospilota</em>: in females, cell cycle activation likely drove oogenesis and ovulation through adenosine depletion-mediated meiotic resumption, while males appeared to exhibit neuromodulated spermatogenesis via caffeine/dopamine signaling. Crucially, we identified phospholipid-mediated membrane stabilization as a potential response to oxidative stress during gamete release. These findings establish the first comprehensive framework for <em>H. leucospilota</em> reproductive physiology, providing actionable targets for aquaculture innovation.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743152"},"PeriodicalIF":3.9,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144989337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimating the effect of wrasses (Labridae) and lumpfish (Cyclopterus lumpus) as control measures against salmon lice in Norwegian fish farms","authors":"Nora R. Aasen ,&nbsp;Solveig Engebretsen ,&nbsp;Magne Aldrin ,&nbsp;Peder A. Jansen","doi":"10.1016/j.aquaculture.2025.743127","DOIUrl":"10.1016/j.aquaculture.2025.743127","url":null,"abstract":"<div><div>Cleaner fish are commonly used as a biological control measure against salmon lice infestations in salmonid farming. However, there have been few attempts at quantifying the effect of cleaner fish on the louse abundance at commercial farms. Our study estimates a delousing effect for wrasses (<em>Labridae</em>) and lumpfish (<em>Cyclopterus lumpus</em>) by fitting a partially stage-structured lice development model to historical production data on all salmonid farms in Norway. We investigate different models, and evaluate them according to a statistical model selection criterion (BIC). The final model includes temperature dependence for the delousing effect of cleaner fish, as a quadratic function. The estimated optimal temperature for lice grazing was found to be 6.7 °C for lumpfish and 15.3 °C for wrasses. The final model also included separate delousing effects for adult female and other motile salmon lice. In general, the estimated delousing effect was larger for wrasses than for lumpfish. However, for temperatures below 8.3 °C, the estimated delousing effect of other motile lice was larger when using lumpfish compared to wrasses. The estimated delousing effect was larger for lower abundances of salmon lice. This implies that cleaner fish should be used at low louse abundances, and not as a delousing method during outbreaks. Our study is an important contribution to quantifying the temperature-dependent delousing effect of cleaner fish, which can be used to guide the farmers in their decision-making when planning cleaner fish strategies.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743127"},"PeriodicalIF":3.9,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144989338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic sex determines recombination rate and distribution in sex-reversed rainbow trout (Oncorhynchus mykiss)","authors":"Cathrine Brekke ,&nbsp;Tim Martin Knutsen","doi":"10.1016/j.aquaculture.2025.743143","DOIUrl":"10.1016/j.aquaculture.2025.743143","url":null,"abstract":"<div><div>During meiotic cell division, homologous chromosomes align and exchange large segments of DNA through crossover recombination. Rates and distribution of crossovers often show distinct differences between males and females, a phenomenon known as heterochiasmy. Some species, such as salmonids, display extreme differences between the sexes in crossover positioning. Despite decades of research documenting the presence of heterochiasmy across eukaryotes, the specific feature of sex leading to this curious sexual dimorphism remains to be explained. A critical part of solving this puzzle is to establish whether heterochiasmy is driven by genetic sex or if it is a result of the physiological differences between producing sperm and eggs. In this study, we show that phenotypic sex determines recombination rate and distribution in hormonally sex-reversed rainbow trout. With pedigree and genotype information from 18,452 individuals and 33,913 SNP markers we map crossover events in families where the fathers were hatched as genetic XX females and sex-reversed as young trout fry with a masculinising hormone 17α-methyltestosterone and compare the crossover patterns to those in families with normal XY male fathers. We find that recombination patterns in XX males resemble those of normal XY males with crossovers exclusively in sub-telomeric regions. Crossover count per gamete was 25.8 ± 4.4 in XX females vs 19.9 ± 4.0 and 19.5 ± 3.9 in XY males and XX males, respectively. These results support the hypothesis that heterochiasmy arises from physiological differences between oogenesis and spermatogenesis rather than effects related to genetic sex and will aid in guiding the research on heterochiasmy going forward.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743143"},"PeriodicalIF":3.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144989347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reducing environmental impacts in tambaqui aquaculture: a life cycle assessment of integrated multitrophic aquaculture with curimba","authors":"Adriana F. Lima ,&nbsp;Joel Aubin ,&nbsp;Wagner C. Valenti","doi":"10.1016/j.aquaculture.2025.743144","DOIUrl":"10.1016/j.aquaculture.2025.743144","url":null,"abstract":"<div><div>This study evaluated the environmental impacts of the integrated multitrophic aquaculture (IMTA) of tambaqui <em>Colossoma macropomum</em> and curimba <em>Prochilodus lineatus</em> compared to tambaqui monoculture in ponds using life cycle assessment (LCA). The assessment is applied to a pilot-scale experiment conducted in 600 m² ponds. Collected data consisted of fish biomass, feed and fertilizer amounts, inlet water volume, electricity consumption, and greenhouse gas emissions, complemented by background data from databases and previous studies. The LCA followed a cradle-to-farm gate approach, quantifying nine impact categories for 1 kg of fish biomass produced. IMTA demonstrated reduced impacts across all categories, with substantial decreases in water dependence (38 %) and freshwater eutrophication (21 %). Additionally, IMTA reduced land occupation by 17 %, acidification and cumulative energy demand by 12 %, marine eutrophication by 11 %, net primary production by 10 %, and climate change impact by 9 %, compared to monoculture. Feed was the primary contributor to energy demand, net primary production, and climate change, while the rearing step (direct culture emissions) was the main contributor to acidification, water dependency, marine eutrophication, and land occupation. IMTA improved feed conversion ratio and nutrient recovery, key factors driving the reduction in environmental impacts. These results highlight IMTA system as a more sustainable alternative to conventional tambaqui monoculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743144"},"PeriodicalIF":3.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}