Aquaculture最新文献

{"title":"The effects of different diets on growth, feeding preference, and intestinal microbiota of juvenile sea cucumber Holothuria leucospilota","authors":"Dejian Su,&nbsp;Hubin Chen,&nbsp;Sizhe Zheng,&nbsp;Yikun Cen,&nbsp;Zonghe Yu","doi":"10.1016/j.aquaculture.2025.742798","DOIUrl":"10.1016/j.aquaculture.2025.742798","url":null,"abstract":"<div><div>Finding an efficient and cost-effective feed is of great significance for the aquaculture and restocking of sea cucumber <em>Holothuria leucospilota</em>. In this study, the performance of juvenile <em>H. leucospilota</em> which were fed with commercial formulated feed (P), was compared with those fed by six different types of algal powders <em>Sargassum</em> sp. (S), <em>Ulva</em> sp. (U), and <em>Laminaria japonica</em> (L)—either solely or in a 1:1 combination (S + U, S + L, U + L). Over a 56-day experiment, we found that the mixed diet of <em>Sargassum</em> sp. and <em>Ulva</em> sp. (S + U) resulted in the highest specific growth rate (4.82 ± 0.34 % d⁻¹), which was comparable to that of the commercial formulated feed (P) (4.30 ± 0.20 % d⁻¹). Isotopic mixing model analysis revealed that <em>H. leucospilota</em> exhibits a preferential assimilation order of <em>Sargassum</em> sp., <em>Ulva</em> sp., and <em>L. japonica</em> when fed mixed diets. Additionally, the intestinal microbiota of sea cucumbers had been influenced by diets, with mixed algal diets improving microbial composition. In the S + U group, which exhibited the fastest growth, the relative abundances of <em>Pseudoruegeria</em>, <em>Labrenzia</em>, <em>Cyanobium PCC-6307</em>, and <em>Megasphaera</em> at the genus level were significantly higher than those in other groups. The results of this study demonstrate that the diet mixed with <em>Sargassum</em> sp. and <em>Ulva</em> sp. is most suitable for juvenile <em>H. leucospilota</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742798"},"PeriodicalIF":3.9,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of PmLyO-Sf9 for in vitro propagation of shrimp nudivirus (Penaeus monodon nudivirus) discovering virus replication, assembly, and release","authors":"D.V. Dominic Diya ,&nbsp;Ritesh Urade ,&nbsp;A. Muneer ,&nbsp;Eldho Linu ,&nbsp;T.P. Sajeevan ,&nbsp;Puthumana Jayesh ,&nbsp;Valsamma Joseph ,&nbsp;Rosamma Philip ,&nbsp;I.S. Bright Singh","doi":"10.1016/j.aquaculture.2025.742702","DOIUrl":"10.1016/j.aquaculture.2025.742702","url":null,"abstract":"<div><div><em>Peneaus monodon</em> nudivirus (PmNV) was successfully isolated on to and propagated in <em>Pm</em>LyO-<em>Sf</em>9, the crustacean-insect hybrid cell line. Cytopathic effects such as clustering, enlargement, syncytium formation, granulation, vacuole formation, tapering or slendering, irregular plasma membrane formation with extended tails, detachment and cell death could be documented. Expression of viral genes such as DNA polymerase, Lef 4 and Polyhedrin depicted the replication of virus in the cell line. Viral polyhedrin gene in the nucleus was detected through fluorescent <em>in situ</em> hybridization (FISH) at 12 h of post-inoculation. Cell culture supernatant turned out infective by the release of virions to it assessed based on its infectivity <em>in vitro</em> and <em>in vivo</em>. PmNV empowered 12–24 h to complete its replication in the cell line. Immediate release of virus from the cells and reinfection in the cells were observed. Clathrin mediated entry of virus to cells was noted through endosomal inhibition assay. Electron micrographs supported the receptor mediated entry, endosomal transport, viroplasm showing active virus replication and assembly of virus inside the nucleus and release as occlusion bodies. Also, prolonged S phase of the cells was notable in virus infected cell line from 24 hpi which explored the virus impediments in the cells for the active replication through flow cytometric analyses.</div><div>This study reveals the potency of the cell line to investigate propagation, host-pathogen interaction, viral morphogenesis which could promotes equitable development of antiviral formulations against the virus while preserving the environment for future generations.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742702"},"PeriodicalIF":3.9,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NOX1-ROS axis regulates the MAPK signaling pathway and activates caspase-1 to resist Edwardsiella piscicida infection","authors":"Xiao Man Wu ,&nbsp;Ming Xian Chang","doi":"10.1016/j.aquaculture.2025.742784","DOIUrl":"10.1016/j.aquaculture.2025.742784","url":null,"abstract":"<div><div>Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase serves as a primary generator of reactive oxygen species (ROS) and assumes a critical role in immune responses, cell proliferation or signal transduction processes. The intricate functionality of ROS endows NADPH oxidase with multifaceted roles in host defense mechanisms. However, the specific functions and underlying mechanisms of fish NADPH oxidase during bacterial infections remain largely elusive. In this research, zebrafish were employed as a model organism to investigate the antibacterial function and mechanism of NOX1. Immunofluorescence and subcellular fractionation analyses demonstrated that zebrafish NOX1 was predominantly localized in the cytoplasm. Moreover, upon infection with <em>Edwardsiella piscicida</em>, a significant upregulation of NOX1 expression was observed. Functional assays revealed that zebrafish NOX1 retained the conserved ability to promote ROS generation. This, in turn, suppressed the growth of <em>E. piscicida</em> through the bactericidal activity of ROS. Consequently, the survival rates of zebrafish and cultured cells were enhanced, accompanied by the upregulation of antibacterial genes. Mechanistic investigations further demonstrated that zebrafish NOX1 exerted its antibacterial effects by promoting the expression of critical genes within the MAPK signaling pathway. Simultaneously, zebrafish NOX1 activated caspase-1 via ROS, causing the induction of inflammatory cytokines. Additionally, the NOX1-ROS axis enhanced the transcriptional upregulation of chemokines, including monocyte chemoattractant protein-1 (<em>mcp-1</em>) and C-X-C motif chemokine ligand 8 (<em>cxcl8</em>), which accelerated neutrophil migration, thereby enabling a rapid response to bacterial infection. In summary, this study comprehensively elucidated the function and mechanism of NOX1 during bacterial infection in teleost. These results shed new light on the understanding of fish immunity and offer potential strategies for the prevention and control of fish diseases.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742784"},"PeriodicalIF":3.9,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlorchinaldol: A promising antiviral agent against iridoviruses in fish","authors":"Jia-Cheng Qin ,&nbsp;Xiao-Ping Tan ,&nbsp;Ying-Jie Jin ,&nbsp;Guang-Shuo Wang ,&nbsp;Shen-Ye Qu ,&nbsp;Peng-Fei Li ,&nbsp;Gao-Xue Wang ,&nbsp;Fei Ling","doi":"10.1016/j.aquaculture.2025.742785","DOIUrl":"10.1016/j.aquaculture.2025.742785","url":null,"abstract":"<div><div>Fish iridoviruses, including the largemouth bass virus (LMBV) and Singapore grouper iridovirus (SGIV), pose severe threats to aquaculture industries, resulting in substantial economic losses. However, no effective treatments are currently available for these viruses, underscoring the critical need to develop high-efficacy therapeutic strategies. This study identified chlorchinaldol as a compound exhibiting significant anti-LMBV activity both <em>in vitro</em> and <em>in vivo</em> in largemouth bass (<em>Micropterus salmoides</em>). Results revealed that chlorchinaldol inhibited LMBV replication in Epithelioma papulosum cyprinid (EPC) cells with a half-maximal inhibitory concentration (IC₅₀) of 9.40 μM, while concurrently reducing viral titers and cytopathic effects (CPE). Mechanistic investigations demonstrated that chlorchinaldol does not directly disrupt LMBV virion integrity but interferes with viral adsorption to host cells and suppresses late-stage viral protein synthesis. Furthermore, chlorchinaldol exhibited both prophylactic and therapeutic efficacy, significantly mitigating LMBV-induced cellular damage. In <em>in vivo</em> experiments, chlorchinaldol administration reduced viral loads in infected organs, ameliorated histopathological damage, upregulated immune-related gene expression, and markedly enhanced survival rates in LMBV-infected largemouth bass. Notably, chlorchinaldol also demonstrated potent anti-SGIV activity in EPC cells, with an IC₅₀ of 10.01 μM and suppression of viral titers and CPE. These findings collectively establish chlorchinaldol, a quinoline derivative, as a broad-spectrum antiviral agent with substantial potential for aquaculture applications.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742785"},"PeriodicalIF":3.9,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment on Chlorella (Chlorella sorokiniana) meal as a substitution for fishmeal in Songpu mirror carp (Cyprinus carpio Songpu) feed: Effect on growth, intestinal health and microflora composition","authors":"Ze Fan ,&nbsp;Yiqi Gao ,&nbsp;Siwei Xiong ,&nbsp;Chenhui Li ,&nbsp;Di Wu ,&nbsp;Jinnan Li ,&nbsp;Liansheng Wang","doi":"10.1016/j.aquaculture.2025.742765","DOIUrl":"10.1016/j.aquaculture.2025.742765","url":null,"abstract":"<div><div>The experiment aims to investigate Chlorella (<em>Chlorella sorokiniana</em>) replaced fishmeal on growth performance, blood biochemical indices and intestinal health of juvenile Songpu mirror carp (<em>Cyprinus carpio</em> Songpu). Chlorella was substituted for 0 % (G1), 30.0 % (G2), 58.3 % (G3) and 88.3 % (G4) of fishmeal (6 %) in the basal diet. Diets were fed in triplicated groups of Songpu mirror carp with an initial body weight of 5.50 ± 0.03 g/fish for 8 weeks. Results showed that the final body weight and specific growth rate increased significantly in G3, and the feed conversion ratio was significantly lower than in G4. The serum albumin content in G3 was significantly higher than in G4; alanine aminotransferase content was significantly higher than remaining groups except G4. Malondialdehyde content decreased significantly with increasing level of substitution. The intestinal villi of G1 and G3 were in the optimal tissue state, neatly arranged and without shedding. The expression levels of genes associated with intestinal protein synthesis (growth hormone, phosphatidylinositol 3 kinase, protein kinase b, s6 kinase), antioxidant (catalase, glutathione peroxidase 1a) and immune (zonula occluden 1, claudin7, claudin11) were significantly higher in G3 than in G1; the intestinal inflammatory factor Interleukin-1β (<em>IL-1β</em>) was significantly lower than G1. The substitution group decreased the relative abundance of <em>Ascomycetes</em> and <em>Actinobacteria</em> phylum and increased the percentage of <em>Firmicutes</em>. This study indicated that Chlorella was substituted for 58.3 % of fishmeal, it could significantly improve the growth performance, enhance the protein metabolism level and promote the development of intestinal morphology and immunity of Songpu mirror carp.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742765"},"PeriodicalIF":3.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examination of post-hatching starvation on oxidative stress response and larval life span in the marine ornamental shrimp, Lysmata seticaudata","authors":"Rafael C. Duarte ,&nbsp;Daniel Acebes ,&nbsp;Diana Madeira ,&nbsp;Ruben X.G. Silva ,&nbsp;Joana F. Fernandes ,&nbsp;Fernando Ricardo ,&nbsp;Ricardo Calado","doi":"10.1016/j.aquaculture.2025.742783","DOIUrl":"10.1016/j.aquaculture.2025.742783","url":null,"abstract":"<div><div>Decapod crustacean larvae typically rely on external food sources for their nutrition, growth, and survival. However, in environments with pronounced seasonality in food availability, early larvae of some species can progress to subsequent larval stages without external feeding, a strategy known as facultative primary lecithotrophy. To save costs associated with the use of live feeds, several producers of marine ornamental shrimp, such as those of genus <em>Lysmata</em>, deprive larvae of food during their first zoeal stage. While this common practice may not directly affect larval survival, it can increase oxidative stress responses in starved larvae and negatively impact future performance, namely at metamorphosis. Such effects, however, are still little explored in species of aquaculture interest. Here, we experimentally assessed the effects of different feeding regimes on newly hatched larvae of the shrimp <em>Lysmata seticaudata</em> using a range of biomarkers of oxidative stress and antioxidant response. We showed that starving newly hatched larvae promoted short-term stress responses at the subsequent larval stage by increasing the activity of oxidative stress enzymes and the total antioxidant capacity of larvae. Such a brief starvation period at the beginning of larval development had significant long-term effects, delaying the time of metamorphosis to post-larvae and resulting in the metamorphosis of larger shrimp. Depriving newly hatched larvae of food, a common practice in aquaculture, is inadvisable as it disrupts the balance between energy used for combating oxidative stress and promote morphogenesis and growth, which may affect post-larval condition by prolonging the duration of the last zoeal stage.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742783"},"PeriodicalIF":3.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From indicator evaluation to optimization decision: Effects of synthetic vs. natural astaxanthin on pigmentation, growth, and health in Penaeus vannamei","authors":"Yucai Xue ,&nbsp;Zhaocai Wang ,&nbsp;Mengting Liu ,&nbsp;Ganfeng Yi ,&nbsp;Xuxiong Huang ,&nbsp;Weilong Wang","doi":"10.1016/j.aquaculture.2025.742782","DOIUrl":"10.1016/j.aquaculture.2025.742782","url":null,"abstract":"<div><div>Astaxanthin, a powerful antioxidant carotenoid, is widely used in aquaculture to promote growth, pigmentation, health and visual appeal of aquatic animals. Natural and synthetic sources differ in isomeric forms, esterification, and processing, affecting their stability, bioavailability, and physiological functions. This study presents a unified framework for evaluating synthetic astaxanthin (Syn) and natural sources derived from marine bacteria <em>Paracoccus carotinifaciens</em> (Mb), microalgae <em>Haematococcus pluvialis</em> (Hp), and yeast <em>Xanthophyllomyces dendrnrhous</em> (Pr).</div><div>A 56-day feeding trial was conducted in <em>Penaeus vannamei</em>, assessing pigmentation, growth, antioxidative capacity, immune response, and stress tolerance using ANOVA, PCA, PLS-DA, and TOPSIS. A total of 80 differential indicators were identified. PCA-TOPSIS analysis revealed that astaxanthin supplementation significantly enhanced antioxidative capacity, stress resistance, metabolism, serum enzyme activity, digestion, and body coloration.</div><div>Synthetic astaxanthin outperformed natural sources in carotenoid content, digestibility, and metabolic parameters, while natural sources excelled in body coloration, antioxidative and anti-stress capacities, serum enzyme activity, and flavor compounds. Although both synthetic and natural sources achieved similar overall scores, each exhibited unique strengths, suggesting that a combined approach could yield enhanced benefits. Mathematical modeling and optimization algorithms identified optimal combinations, including 0.011 g/kg Hp, 1.2 g/kg Syn, and 26.34 g/kg Pr (Ast %: 0.5:60:39.5) for synthetic astaxanthin, and 0.12 g/kg Hp and 63.07 g/kg Pr (Ast%: 5.4:94.6) for natural astaxanthin.</div><div>This framework provides practical guidance for optimizing aquaculture feed formulations, enhancing nutritional efficiency, cost-effectiveness, and product quality. By integrating data-driven approaches, this study contributes to the sustainable development of aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742782"},"PeriodicalIF":3.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms of Larimichthys crocea's adaptation to fishmeal-free diets: Insights from liver proteomics and functional analysis of Alpha-2-HS-glycoprotein","authors":"Zhenye Zhao ,&nbsp;Peng Ren ,&nbsp;Ying Huang ,&nbsp;Gongsi Wang ,&nbsp;Jiawei Zhang ,&nbsp;Fang Han ,&nbsp;Zhiyong Wang","doi":"10.1016/j.aquaculture.2025.742774","DOIUrl":"10.1016/j.aquaculture.2025.742774","url":null,"abstract":"<div><div>The depletion of global fishery resources has led to a shortage in fishmeal supply, necessitating the exploration of fishmeal-free diets in aquaculture. Large yellow croaker (<em>Larimichthys crocea</em>) is a significant species in aquaculture but is constrained by fishmeal availability. In this study, large yellow croaker individuals fed a fishmeal-free diet were screened for comparable feeding rates but varying feed efficiency, followed by Data-Independent Acquisition (DIA) -based liver proteomic sequencing. Differentially expressed proteins (DEPs) were enriched in lipid metabolism pathways. High feed efficiency (H) groups exhibited upregulation of proteins related to lipid transport and cholesterol synthesis, indicating superior lipid utilization. The fishmeal-free diet, rich in phytosterols, enhanced the H group's lipid transport capability by activating proteins such as Apoa1 and Apoa4, which also exhibited anti-inflammatory properties. Notably, α-2-HS-glycoprotein (Ahsg) was significantly upregulated. Functional studies using recombinant LcAhsg protein in an intestinal inflammation model revealed its capacity to mitigate inflammation. Overexpression of <em>Lcahsg</em> regulated inflammation-associated genes, maintaining liver function and optimizing feed utilization. This study elucidates the molecular mechanisms underlying the adaptation of <em>L. crocea</em> to fishmeal-free diets, providing a scientific basis for aquaculture optimization.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742774"},"PeriodicalIF":3.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The multi-omics approach reveals the impact of Temnocephala digitata infection on Macrobrachium rosenbergii","authors":"Lianggang Wang ,&nbsp;Yan Yang ,&nbsp;Libin Huang ,&nbsp;Guanghua Huang ,&nbsp;Lan Zeng ,&nbsp;Xiaohua Lu ,&nbsp;Zhide Ruan ,&nbsp;Shaokui Yi ,&nbsp;Quanxin Gao ,&nbsp;Tianhe Lu ,&nbsp;Jingkai Qin ,&nbsp;Liuchun Lan ,&nbsp;Rui Wang ,&nbsp;Liming Huang","doi":"10.1016/j.aquaculture.2025.742769","DOIUrl":"10.1016/j.aquaculture.2025.742769","url":null,"abstract":"<div><div><em>Macrobrachium rosenbergii</em> (<em>M. rosenbergii</em>) is a vital freshwater economic species, and no prior reports exist on its infection by the symbiotic flatworm <em>Temnocephala digitata</em> (Platyhelminthes: Temnocephalidae; <em>T. digitata</em>). However, during aquaculture practices, we observed that <em>T. digitata</em> infection caused growth retardation and even mortality in <em>M. rosenbergii</em>. This study investigates the pathogenic mechanisms of <em>T. digitata</em> infection in <em>M. rosenbergii</em> using a multi-omics approach. Results revealed that <em>T. digitata</em> infection induced growth retardation, immune suppression, and metabolic dysregulation in the shrimp. Intestinal microbiota analysis demonstrated microbial dysbiosis characterized by an increased abundance of Proteobacteria (particularly the opportunistic pathogenic genus <em>Aeromonas</em>) and reduced proportions of Firmicutes and Bacteroidota. Integrated transcriptomic and metabolomic analyses showed that <em>T. digitata</em> infection suppressed ribosomal functions, lipid metabolism, and protein metabolism in gill tissues while concurrently activating inflammatory factors and pathways (e.g., TNF-α, COX-2, and Toll/IMD signaling). Additionally, infection induced abnormal molting behavior by upregulating ecdysone (ECD), and methyl farnesoate (MF) while suppressing molt-inhibiting hormone (MIH), exacerbating energy depletion and immune exhaustion. Multi-omics correlation analysis further elucidated complex interactions among core gut microbiota, key metabolites, and hub genes, collectively driving nutrient-immune imbalance. Histopathological examinations confirmed hepatopancreatic vacuolization, intestinal villi atrophy, and gill lesions, consistent with metabolic and immune dysfunction. This study provides the first evidence of <em>T. digitata</em>-associated pathogenicity in <em>M. rosenbergii</em>, revealing that the symbiont-host interaction disrupts nutrient metabolism-immune homeostasis, thereby precipitating a health crisis. These findings advance understanding of temnocephalid-crustacean relationships and highlight risks of symbiont overproliferation in aquaculture systems.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742769"},"PeriodicalIF":3.9,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Techno-economic assessment of ear-hanging and lantern net grow-out techniques in Atlantic Sea scallop, Placopecten magellanicus, aquaculture in the Gulf of Maine","authors":"Christopher Noren ,&nbsp;Struan Coleman ,&nbsp;Christian Brayden ,&nbsp;Andrew Chingos ,&nbsp;Dana Morse ,&nbsp;Andrew Peters ,&nbsp;Damian C. Brady","doi":"10.1016/j.aquaculture.2025.742775","DOIUrl":"10.1016/j.aquaculture.2025.742775","url":null,"abstract":"<div><div>Global scallop production has rapidly transitioned from a wild-capture fishery to an aquaculture industry over the past several decades. However, aquaculture of the Atlantic sea scallop (<em>Placopecten magellanicus</em>) in the Gulf of Maine has remained limited by the high labor burden and costs within the United States and Canada, particularly when using traditional lantern net culture. As a result, specialized ear-hanging equipment designed to automate husbandry processes is increasingly being employed in scallop aquaculture to reduce labor, despite the higher initial investment. Here, we used a techno-economic model to compare the cost of production, net present value, modified internal rate of return, lease size requirements, and labor-bounded maximum annual production of automated ear-hanging and traditional lantern net culture, production cycle duration, and market products. While ear-hanging entailed higher initial capital expenditures, it was notably more cost effective compared to lantern net culture; the advantages were compounded at larger production scales, longer production cycle durations, and when targeting an adductor muscle market. Labor efficiencies in ear-hanging related to a total annual production capacity of almost double that for lantern net culture and a lease acreage reduction of 40 % at comparable annual production. We recommend that growers looking to scale scallop production (&gt;100,000 annual production) consider automated ear-hanging targeting an adductor muscle product. Meanwhile small-scale growers (&lt;100,000 annual production) would likely need to adjust assumptions for a profitable business model. To assist growers in this decision-making process, we have included a scenario-testing application to adjust assumptions to fit their specific business requirements.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742775"},"PeriodicalIF":3.9,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}