Aquaculture最新文献

{"title":"Identification of a novel strain of carp edema virus affecting Songpu mirror carp in Liaoning, China","authors":"Wen Shi ,&nbsp;Bing Han ,&nbsp;Huilin He ,&nbsp;Ruixue Feng ,&nbsp;Siyi Guo ,&nbsp;Xuanyu Ren ,&nbsp;Jinshan Huang ,&nbsp;Xu Lin ,&nbsp;Jiyuan Yin ,&nbsp;Xiaonan Lv ,&nbsp;Min Liu","doi":"10.1016/j.aquaculture.2025.742151","DOIUrl":"10.1016/j.aquaculture.2025.742151","url":null,"abstract":"<div><div>Carp edema virus disease (CEVD), also referred to as koi sleepy disease (KSD), is a viral infectious disease caused by the carp edema virus (CEV). It is primarily characterized by lethargy, generalized edema, and gill hyperplasia and necrosis. The disease has a widespread distribution and is associated with high mortality, leading to significant economic losses within the carp aquaculture industry. This study aims to identify the pathogenic agents affecting Songpu mirror carp (<em>Cyprinus carpio Songpu</em>) from a carp farm experiencing elevated mortality rates in Liaoning Province, China, which experienced elevated mortality in late May 2018. Bacterial and parasitic infections were systematically excluded, and the presence of CEV infection in Songpu mirror carp was confirmed through nested PCR identification. The isolate was named CEV L-3 strain. The <em>p4a</em> partial gene sequence obtained was analyzed, which revealed that L-3 strain exhibits the highest homology compared with the UK isolate R083, which is classified within the IIa genotype. It was observed that the L-3 strain demonstrates a closer phylogenetic relationship to M141 from the UK and 112–2015 from Poland. Additionally, the gills were identified as the target organ of the L-3 strain by real-time PCR, which exhibited the highest viral load, followed by the kidney and spleen. This study has identified a novel strain of CEV which is prevalent in China, thereby paving the way for further research into the epidemiological characteristics, diagnostics and viral biological properties of CEVD.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742151"},"PeriodicalIF":3.9,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dihydroartemisinin inhibits Micropterus salmoides rhabdovirus infection in vitro and in vivo","authors":"Yuanyuan Li ,&nbsp;Peipei Zhang ,&nbsp;Shuangshuang Mao ,&nbsp;Xiaoran Feng ,&nbsp;Haixiang Liu ,&nbsp;Si Deng ,&nbsp;Lunguang Yao ,&nbsp;Yinghui Qin","doi":"10.1016/j.aquaculture.2025.742163","DOIUrl":"10.1016/j.aquaculture.2025.742163","url":null,"abstract":"<div><div><em>Micropterus salmoides</em> rhabdovirus (MSRV) poses a significant threat to largemouth bass farming. Currently, there are no licensed vaccines or antiviral drugs available to prevent MSRV infection. Dihydroartemisinin (DHA), an active metabolite of artemisinin, possesses widespread pharmacological activities, such as anti-tumor, anti-malaria, and anti-virus functions. The study aimed to investigate the effect of DHA on MSRV replication and its underlying mechanism. Cell viability assay and drug treatment showed that DHA significantly inhibited MSRV replication at a concentration as low as 2 μM. Furthermore, the inhibitory effect of DHA increased in a concentration-dependent manner within the range of 2–40 μM without obvious cytotoxicity. The disinfection test indicated DHA did not directly inactivate MSRV virus. The pretreatment assay indicated that treating EPC cells with DHA before MSRV infection did not inhibit MSRV replication, suggesting the antiviral function of DHA might not target MSRV receptor or not induce host cells to produce antiviral factors. Time of addition assays suggested that DHA functioned throughout the entire life cycle of MSRV, particularly in the early stage. Mechanistically, DHA inhibited MSRV internalization, genomic transcription and replication, assembly, and release, but did not inhibit MSRV attachment. Further studies revealed that DHA did not decrease the mRNA levels of clathrin and dynamin, both of which play crucial roles in MSRV internalization. In vivo experiments showed that DHA significantly improved the survival rate of MSRV-challenged fish by 36.8 %. Altogether, DHA exhibits a strong anti-MSRV function and has promising application prospects in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742163"},"PeriodicalIF":3.9,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of food addiction of lysine on feeding habit in fish","authors":"Hong-Liang Lu ,&nbsp;Yun-Liang Miao ,&nbsp;Jia-Ming Zou ,&nbsp;Zi-Jun Hu ,&nbsp;Ying-Cen Li ,&nbsp;Xu-Fang Liang ,&nbsp;Shan He","doi":"10.1016/j.aquaculture.2025.742160","DOIUrl":"10.1016/j.aquaculture.2025.742160","url":null,"abstract":"<div><div>The current researches on food addiction focus on the diagnostic criteria, neurobiological theory, relationship of obesity and treatment. However, little is reported on the relationship between food addiction and feeding habit, especially in fish. In the present study, <em>in vitro</em>, the signaling pathway of food addiction of lysine and glucose in the brain cells of Chinese perch (<em>Siniperca chuatsi</em>) was determined. <em>In vivo</em>, the behavior experiment of food addiction was conducted on zebrafish (<em>Danio rerio</em>). <em>In vitro</em>, we found that lysine had a positive regulatory effect on food addiction signaling pathway and orexigenic factor Agrp expression, and the effect was suppressed with the inhibitors treatment of the signaling c-Fos/Mor/Drd1, whereas glucose exhibited a contrary trend. <em>In vivo</em>, the zebrafish were fed with the diets containing the normal lysine/carbohydrate level (Control Group1/2, CG1/2) or high lysine/carbohydrate level (Food Addiction Group1/2, FAG1/2) for 31 days during the food addiction formation. At Day 7, 12, 18, 23, 28 and 31, the food intake experiment showed that the FAG1 fish ingested the gelatin bait containing the high lysine diets more than the CG1 fish, and the increase trend was eliminated by the inhibitor of Mor (FAG1 + inhi). In the food selection experiment, the FAG1 fish preferred to ingest the gelatin bait containing the high lysine diets more than that containing the control diets, and the increase trend was eliminated in the FAG1 + inhi fish. Zebrafish existed the lysine addiction, which could modulate the food selection through the signaling pathway of food addiction and Agrp. Our data illustrated for the first time that food addiction to nutrients such as lysine exists in fish and that it is regulated by the food addiction signaling pathway c-Fos/Mor/Drd1/Agrp.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742160"},"PeriodicalIF":3.9,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143352747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of funnel-shaped recirculating aquaculture system on the growth, health, and muscle quality of largemouth bass (Micropterus salmoides)","authors":"Jiaxin Xu ,&nbsp;Chang Liu ,&nbsp;Jiajun Zheng ,&nbsp;Jianhua Wang ,&nbsp;Lingran Wang ,&nbsp;Di Feng ,&nbsp;Meng Zhang ,&nbsp;Miao Yu ,&nbsp;Hongxia Jiang ,&nbsp;Zhigang Qiao ,&nbsp;Lei Wang","doi":"10.1016/j.aquaculture.2025.742176","DOIUrl":"10.1016/j.aquaculture.2025.742176","url":null,"abstract":"<div><div>This study assessed the impact of Traditional Pond Aquaculture Systems (TPAS) and Funnel-shaped Recirculating Aquaculture Systems (FRAS) on the growth performance, physiological health, and muscle quality of largemouth bass (<em>Micropterus salmoides</em>). The results indicated that the FRAS group exhibited extremely significant higher body weight in largemouth bass compared to the TPAS group (<em>P</em> &lt; 0.01). The FRAS group demonstrated superior liver antioxidant capacity, accompanied by significantly higher amylase and lipase activities in intestinal digestive enzymes. Additionally, the FRAS group showed significantly improved muscle hardness, elasticity, and fiber density, along with a notable decrease in muscle-saturated fatty acid content compared to the TPAS group (<em>P</em> &lt; 0.01). The FRAS group displayed a markedly higher essential amino acid content in muscle compared to the TPAS group (<em>P</em> &lt; 0.05). Regarding volatile compounds, the FRAS group notably enhanced the sensory quality of fish muscle by removing undesirable odors and producing a more appealing aroma. This study comprehensively examined differences in growth performance, physiological health, and muscle quality of largemouth bass between the TPAS and FRAS. The findings provide valuable insights and solid scientific evidence to promote largemouth bass farming within the FRAS system.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742176"},"PeriodicalIF":3.9,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143292601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and characterization of a monoclonal antibody against MCP of largemouth bass ranavirus and investigation of the virus's tissue tropism","authors":"Yuhui Wang ,&nbsp;Yaoda Wang ,&nbsp;Tianmei Hu ,&nbsp;Hongye Cui ,&nbsp;Long Huang ,&nbsp;Haiyue Chen ,&nbsp;Baozhou Wu ,&nbsp;Shuguang Hao ,&nbsp;Wenlong Cai ,&nbsp;Yali Wu ,&nbsp;Jiping Zhang ,&nbsp;Weiwei Zeng","doi":"10.1016/j.aquaculture.2025.742170","DOIUrl":"10.1016/j.aquaculture.2025.742170","url":null,"abstract":"<div><div>Largemouth bass ranavirus (LMBV) is a highly contagious pathogen causing severe symptoms and significant mortality in freshwater fish. With no effective vaccine or treatment, rapid detection and control are critical for managing outbreaks. In this study, we report the development and characterization of monoclonal antibodies (mAbs) targeting the major capsid protein (MCP) of the LMBV. Furthermore, these mAbs, in conjunction with quantitative polymerase chain reaction (qPCR) techniques, were employed to investigate tissue tropism and distribution following LMBV infection in largemouth bass. The findings revealed that three hybridoma cell lines, designated as 45ED8, 9F5F2 and 20D7, consistently secreted anti-MCP mAbs. The 45ED8 and 9F5F2 mAbs were identified as IgG1 subtype antibodies, whereas 20D7 was classified as an IgG2b subtype antibody. All mAbs specifically detected LMBV in western blot (WB), immunofluorescence assay (IFA), and immunohistochemistry (IHC) without cross-reacting with other fish pathogens. One of the mAbs demonstrated significant neutralizing activity against LMBV, with a neutralization titer of 1:128. IHC analysis revealed positive signals in all tested samples from LMBV-infected fish, with particularly strong signals observed in the liver, spleen, and intestines. These findings are consistent with qPCR results. In light of the current scarcity of widely accessible reagents for LMBV, these specific mAbs hold potential for advancing research and establishing a foundation for novel diagnostic and therapeutic approaches. Furthermore, the observed tissue tropism enhances our understanding of the pathogenesis and host-virus interactions of LMBV, thereby informing future research endeavors.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742170"},"PeriodicalIF":3.9,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of hypoxic stress on gastric sieve (GS) filtering function of Litopenaeus vannamei","authors":"Letian Li ,&nbsp;Yuan Wang ,&nbsp;Laizhuang Jin ,&nbsp;Lei Feng ,&nbsp;Qianhui Song ,&nbsp;Di Fang ,&nbsp;Na Ying ,&nbsp;Xincang Li ,&nbsp;Wenhong Fang","doi":"10.1016/j.aquaculture.2025.742169","DOIUrl":"10.1016/j.aquaculture.2025.742169","url":null,"abstract":"<div><div>The gastric sieve (GS) is a filtering structure found in the posterior stomach chamber (PSC) of <em>Litopenaeus vannamei</em>, which allows small particles to enter the hepatopancreas directly. However, it remains unclear if pathogens can also enter the hepatopancreas through this pathway. In this study, we examined the internal structural changes of the PSC via histological and ultra-structure analysis. We also orally administered fluorescent microspheres and bioparticles (<em>Vibrio parahaemolyticus</em> and WSSV) of varying sizes to shrimp to determine their passage under hypoxia stress. The results showed that the PSC could be divided into four segments based on structural changes, and the GS performed its filtering function through two kinds of setae with different morphologies. Under normal dissolved oxygen levels (approximately 7.6 mg/L), <em>V. parahaemolyticus</em> and 1 μm fluorescent microspheres were unable to enter the hepatopancreas via GS. In contrast, under hypoxia stress (1.5–2 mg/L), both vibrio and 1 μm fluorescent microspheres could enter the hepatopancreas via GS in a short timeframe. WSSV and 0.1 μm fluorescent microspheres could enter the hepatopancreas via GS irrespective of hypoxia stress, while 5 μm fluorescent microspheres could not enter the hepatopancreas via GS even under hypoxia. These results suggest that GS functions as a particle diameter-dependent filter, with its filtering function influenced by hypoxia stress. Pathogens around 1 μm in size can invade the hepatopancreas through this pathway under hypoxia stress, providing novel insights into the mechanisms of pathogen invasion in shrimp.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742169"},"PeriodicalIF":3.9,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vulnerability and epidemic spread on the Tongyeong coast: A spatiotemporal hybrid model comparison of disease transmission scenarios in aquaculture","authors":"Gyoungsik Kang,&nbsp;HyeongJin Roh","doi":"10.1016/j.aquaculture.2025.742164","DOIUrl":"10.1016/j.aquaculture.2025.742164","url":null,"abstract":"<div><div>The sea cage farming system is one of the most common methods used for culturing fin-fish in South Korea. The region where these fish have been cultured most intensively using sea-cages is the Tongyeong coast. However, assessments of the risk of disease transmission on the Tongyeong coast that consider multiple epidemiological factors have, to date, not been sufficiently addressed. To address this, the current study utilized a spatiotemporal hybrid model to evaluate the risk of infectious disease transmission under various scenarios in fish farms along the Tongyeong coast. Based on their geographical location, 87 fish farms were divided into three bay management areas, A, B, and C (BMA A-C). To evaluate the vulnerability of disease transmission based on the location of each fish farm, 2175 disease outbreak simulations were conducted using different <em>ScalingInf</em> that reflect the chance of widespread disease transmission. The spread of disease along the Tongyeong coast was then predicted under different <em>ScalingInf</em> values and different host susceptibility for outbreaks occurring in each fish farm belonging to one of the three BMAs. The findings revealed that the central region of fish farm BMA A exhibited a 2 to 10 times higher likelihood of disease transmission in the worst-case scenario, highlighting its vulnerability compared with other areas. Overall, the extent of disease spread varied depending on where the outbreak occurred and the <em>ScalingInf</em> values. Outbreaks at fish farms in BMA A or B significantly impacted most farms within BMAs A and B, whereas those in BMA C had a relatively minor effect. Likewise, we found that differences in host susceptibility can significantly impact disease transmission among fish farms. Notwithstanding the limitations of the model, such as its inability to fully account for vector-based transmission and complex interactions between pathogens, hosts, and environmental factors, it provides valuable insights that can be used for predicting susceptible regions and optimizing fish farm management.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742164"},"PeriodicalIF":3.9,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of different artificial infection method models for grass carp reovirus","authors":"Hanyue Wang ,&nbsp;Yongming Li ,&nbsp;Qian Wang ,&nbsp;Bin Gui ,&nbsp;Lanjie Liao ,&nbsp;Zuoyan Zhu ,&nbsp;Yaping Wang ,&nbsp;Rong Huang ,&nbsp;Libo He","doi":"10.1016/j.aquaculture.2025.742162","DOIUrl":"10.1016/j.aquaculture.2025.742162","url":null,"abstract":"<div><div>Grass carp reovirus (GCRV) causes hemorrhagic disease leading to substantial economic losses to the aquaculture industry of grass carp. Effective artificial virus infection methods are essential for the disease-resistant breeding programs and vaccines development. In our study, we established four distinct artificial infection methods for GCRV to identify the most effective approach for inducing infection in grass carp. These methods included feeding, gavage, immersion, and injection. Each method resulted in a similar mortality rate of approximately 80 % and induced characteristic hemorrhagic symptoms associated with GCRV infection. The viral replication patterns were consistent across all infection methods in eight examined tissues, with the intestine, brain, liver, and muscle identified as particularly susceptible to GCRV infection. Furthermore, all artificial infection methods led to significant pathological alterations in the liver and intestinal tissues. Notably, a large number of GCRV viral particles were observed in the kidneys of the infected groups but not present in the control groups. These results underscore the efficacy of the four artificial infection methods evaluated. The choice of the optimal infection method should be tailored to specific conditions. Our findings provide valuable insights for disease-resistant breeding and vaccines development, thereby contributing to the sustainability of the aquaculture industry.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742162"},"PeriodicalIF":3.9,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of low-protein diets with varying carbohydrate-to-lipid ratios on growth performance, antioxidant activity of Litopenaeus vannamei, and microbiome in biofloc systems","authors":"Yunqi Zhang ,&nbsp;Wenping Feng,&nbsp;Hanying Xu,&nbsp;Jiteng Wang,&nbsp;Yulong Sun,&nbsp;Han Wang,&nbsp;Xueting Liu,&nbsp;Tao Han","doi":"10.1016/j.aquaculture.2025.742122","DOIUrl":"10.1016/j.aquaculture.2025.742122","url":null,"abstract":"<div><div>A 53-day experiment investigated the impact of varying dietary carbohydrate-to-lipid (C/L) ratios in biofloc technology (BFT) systems on the growth, feed efficiency, enzyme activity, and metabolism of Pacific white shrimp <em>Litopenaeus vannamei</em>, while also analyzing the microbial community structure and function in the biofloc. Five isonitrogenous and isoenergetic diets (C/L 1.0, 4.1, 6.2, 10.5 and 14.2, 29 % crude protein) were formulated, along with a control diet (35 % crude protein, 7 % lipid). In the groups with dietary C/L ratio of 4.1 and 6.2 group, shrimp showed improved nitrogen retention (NR), energy retention (ER), and higher trypsin activities, compared to the control group. As the C/L ratio increased from 1.0 to 10.5, haemolymph glucose levels increased significantly, while triglyceride and total cholesterol levels were highest at a C/L ratio of 4.1, subsequently decreasing significantly with further increases in the C/L ratio. Antioxidant enzyme activities (CAT and SOD) were elevated in the BFT groups, with the lowest malondialdehyde (MDA) levels observed at C/L ratios of 4.1 and 6.2. Pyruvate kinase (PK) and phosphofructokinase (PFK) activities increased with increasing C/L ratio, while phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) activities decreased. There were no significant differences in the microbial composition, function, or nutrient content of the bioflocs across BFT groups, all of which maintained water quality and provided supplemental nutrition for shrimp. Based on quadratic fitting of weight gain (WG) and specific growth rate (SGR), the optimal dietary C/L ratio for L. <em>vannamei</em> in biofloc systems ranged from 6.44 to 6.52. This study provides theoretical guidance for formulating low-protein and low-cost feeds for farming <em>Litopenaeus vannamei</em> in biofloc systems.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742122"},"PeriodicalIF":3.9,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143292599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial and histopathological insights into gill health of Atlantic salmon (Salmo salar) across Scottish aquaculture sites","authors":"Eoin Costelloe ,&nbsp;Marlene Lorgen-Ritchie ,&nbsp;Elżbieta Król ,&nbsp;Patricia Noguera ,&nbsp;Ralph Bickerdike ,&nbsp;John Tinsley ,&nbsp;Victoria Valdenegro ,&nbsp;Alex Douglas ,&nbsp;Samuel A.M. Martin","doi":"10.1016/j.aquaculture.2025.742166","DOIUrl":"10.1016/j.aquaculture.2025.742166","url":null,"abstract":"<div><div>The increasing prevalence of gill diseases in sea-farmed Atlantic salmon (<em>Salmo salar</em>) poses significant challenges to the aquaculture industry, affecting both fish welfare and economic productivity. This study investigates the association between gill microbiota and histopathological health in Atlantic salmon across three Scottish marine production sites. Gill mucus samples were collected and analysed using 16S rRNA sequencing to profile microbial communities, in parallel with histopathological examinations of gill tissue. The findings reveal rich and diverse microbial communities in gills with low histopathology, with a notable decrease in microbial diversity associated with increased prevalence of histopathological markers. Genera including <em>Candidatus Branchiomonas</em> and <em>Candidatus Rubidus</em> were more prevalent in gills with higher pathology scores, implicating these genera as potential microbial indicators of gill disease. These results suggest that specific microbial profiles could serve as potential measurable biological indicators or biomarkers for early detection and management of gill diseases in aquaculture. This study enhances our understanding of gill microbiome dynamics and their implications for fish health management in marine aquaculture environments.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742166"},"PeriodicalIF":3.9,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}