Aquaculture最新文献

{"title":"Effects of high temperature on gonadal development of gynogenetic Megalobrama amblycephala and acquisition of fertile pseudo-male fish","authors":"Wen Fu ,&nbsp;Ze Li ,&nbsp;Xin Ren ,&nbsp;Feng Liu ,&nbsp;Wenbin Liu ,&nbsp;Jinhui Liu ,&nbsp;Liangyue Peng ,&nbsp;Yamei Xiao","doi":"10.1016/j.aquaculture.2025.742588","DOIUrl":"10.1016/j.aquaculture.2025.742588","url":null,"abstract":"<div><div>Artificial gynogenesis provides one of efficient strategies for fish germplasm improvement. But in aquaculture practice, it is still difficult to expand the offspring population and breeding scale owing to the unisexuality of gynogenetic strains. This study indicates that the gynogenesis blunt snout bream (GBSB), an all-female population, can be sex reversal to the pseudo-male at high temperatures of 34 ± 1 °C. By hormone test experiments on GBSB, it is validated that higher temperature causes a decreasing estrogen level and an increasing androgen level. Observation results also show that the two-year old pseudo male GBSB reaches sexual maturity and produces functional haploid sperms. Genotypic sex identification further confirms that the absence of male genetic marker for blunt snout bream in the pseudo-male GBSB. It is concluded that high-temperature cultivation is an effective strategy of inducing the sex reversal of GBSB, and the results can lay a foundation for large scale breeding of gynogenesis strains in fish.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742588"},"PeriodicalIF":3.9,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aeration causes lordosis in largemouth bass and blunt snout bream and identification of the associated genes","authors":"Haijie Chen ,&nbsp;Leyang Peng ,&nbsp;Jing Wang ,&nbsp;Cheng Luo ,&nbsp;Hui Qiao ,&nbsp;Guili Song ,&nbsp;Shimei Lin ,&nbsp;Yong Long","doi":"10.1016/j.aquaculture.2025.742590","DOIUrl":"10.1016/j.aquaculture.2025.742590","url":null,"abstract":"<div><div>Skeletal deformities in fish pose a significant economic challenge to the aquaculture industry. Lordosis, a common trunk malformation, affects various farmed fish species, but its exact causes and genetic mechanisms remain unclear. High rates of lordosis were observed in largemouth bass (<em>Micropterus salmoides</em>) and blunt snout bream (<em>Megalobrama amblycephala</em>) fries raised in laboratory recycling aquaria systems, providing valuable research models for exploring the causal factors and the associated genes. Alizarin red staining of lordotic fish revealed an obvious inward curvature of the vertebrae. Body measurements revealed lower body weight in lordotic largemouth bass (LMB) fries compared to normal ones. RNA-seq analysis of trunk tissues from normal and deformed LMB fries identified 118 upregulated genes and 78 downregulated genes in the deformed fish. The upregulation of <em>ostn</em> (Osteocrin), a gene responsive to mechanical forces and involved in bone growth, suggested aeration as a potential cause of lordosis. To test this hypothesis, larvae of LMB and blunt snout bream were raised in glass aquaria with or without aeration, confirming that aeration induced lordosis in both species. Genes associated with bone development, including the <em>ostn</em>, <em>ch25h</em>, <em>alx1</em>, and <em>notum2</em>, showed sensitivity to aeration, with a dose-dependent effect on the occurrence of lordosis in blunt snout bream fries. Furthermore, a field investigation revealed a correlation between aeration and the occurrence of lordosis in large-scale farmed LMB fries. Our findings highlight aeration as a causal factor for lordosis in LMB and blunt snout bream and provide insights into associated genes for the first time. Given the widespread use of aeration in intensive aquaculture, particularly during larvae rearing, our findings offer valuable insights for the industry to prevent aeration-induced lordosis in farmed fish.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742590"},"PeriodicalIF":3.9,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of Yersinia ruckeri serotype O1 biotype 1 and biotype 2 isolates from rainbow trout in Turkey: Proteomic, serologic, antigenic, genomic, and virulence characteristics","authors":"Soner Altun ,&nbsp;Izzet Burcin Saticioglu ,&nbsp;Nihed Ajmi ,&nbsp;Gorkem Tasci ,&nbsp;Cuneyt Ozakin ,&nbsp;Nazmiye Ulku Tuzemen ,&nbsp;Jesús L. Romalde ,&nbsp;Sebahattin Ergün ,&nbsp;Sevdan Yılmaz ,&nbsp;Muhammed Duman","doi":"10.1016/j.aquaculture.2025.742587","DOIUrl":"10.1016/j.aquaculture.2025.742587","url":null,"abstract":"<div><div>Enteric Red Mouth (ERM) disease caused by <em>Yersinia ruckeri</em> first emerged in rainbow trout in the United States in the 1950s. Despite the development of effective vaccines in the 1970s, ERM continues to spread rapidly worldwide (Europe, Asia, Africa, Australia, and New Zealand), causing significant mortality, particularly in salmonid fish. In this study, a total of 69 <em>Y. ruckeri</em> isolates collected from rainbow trout between 2013 and 2023, along with two reference strains (NCTC 12266 and the field strain Biotype 2 [BT2]), were subjected to phenotypic tests such as motility, lipase activity, and sorbitol fermentation to determine biotypes (BT). The isolates were characterized as serotype O1 Biotype 1 (BT1) (<em>n</em> = 46) and BT2 (<em>n</em> = 23) using motility test to detect bacterial motility, SDS-PAGE and Western blot analyses to profile whole-cell proteins and antigenic properties, and slide and microagglutination tests to assess serological relationships. While general similarities were observed in the whole-cell protein profiles, significant differences were found in the antigenic protein patterns. <em>Y. ruckeri</em> strains of serotype O1 BT1 and BT2 showed strong cross-reactions in slide agglutination and quantitative agglutination tests, with titers ranging from 1/160 to 1/1280, except for the Y2022-239 strain. It was determined that the isolates Y2022-266 from O1 BT1 and Y75-2013 from O1 B2 displayed a distinct (1/1280) antibody titer compared to other isolates. Additionally, comprehensive genomic analyses were performed to compare the overall genomic relatedness, virulence genes, and antimicrobial resistance patterns of these biotypes. Genomic analyses revealed that the Turkish <em>Y. ruckeri</em> serotype O1 BT1 and BT2 strains formed a common cluster distinct from isolates of different geographical origins. Furthermore, the <em>Y. ruckeri</em> O1 BT1 (<em>n</em> = 3) and O1 BT2 (<em>n</em> = 5) strains diverged into two subclusters. The AMR genes among these strains were completely similar. The virulence capabilities of pathogenic isolates exhibiting serotype O1 BT1 and BT2 characteristics were also investigated in vivo. The Kaplan–Meier survivorship curves indicate that specific strains within the serotype O1 BT1 and BT2 groups exhibit varying levels of virulence, with BT2 strains showing significantly lower survival rates in rainbow trout than the BT1 strains (<em>p</em> &lt; 0.05). This study is the first to determine the serological, antigenic, and virulence characteristics of <em>Yersinia ruckeri</em> serotype O1 BT1 and BT2 strains isolated in Turkey.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742587"},"PeriodicalIF":3.9,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aquaculture nutrition of perch and pikeperch: An analysis for European percid aquaculture","authors":"Tomáš Pěnka ,&nbsp;Anıl Axel Tellbüscher ,&nbsp;Jan Mráz,&nbsp;Tomáš Policar,&nbsp;Koushik Roy","doi":"10.1016/j.aquaculture.2025.742589","DOIUrl":"10.1016/j.aquaculture.2025.742589","url":null,"abstract":"<div><div>Percid aquaculture in Europe, perch (<em>Perca fluviatilis</em>) and pikeperch (<em>Sander lucioperca</em>), is gaining momentum. Substantial knowledge gaps exist in aquaculture nutrition, research and development of European percid diets. We present an analysis to advance the food and feeding of European percids in the European recirculatory aquaculture system (RAS). The highest thermal growth coefficient (TGC) was predicted with the inclusion of crude protein (CP) ~54.5% (2nd maximum ~35%) at non-protein energy (NPE) level ~7.2 MJ kg−1 and at crude energy (CE) level ~17.08 MJ kg−1 (FCR wise). In addition, crude lipid (CL) level of 14.5–15.0% was predicted to maximize growth. A clear maximum for nitrogen-free extract (NFE) or carbohydrates and CE was not found for growth. Estimates of apparent digestibility of organic matter (78.5–85.2 %), protein (90.8–92.7 %), lipid (79–91.8 %), ash (38–62.9 %), and phosphorus (44–59.4 %) in percids can be classified as high. Percid ideal protein concept implies that methionine and histidine could be formulated up to 50 % of lysine within the protein. Protein sources such as fish, shellfish, poultry, insect, potato, and rice form optimum protein choices (&gt;60 % protein utilization) for percid diet formulations. Some complementary protein sources are modeled and proposed to have maximized protein utilization for percids (e.g., combination of poultry, insect, and potato or rice proteins). Body size-adjusted feeding dose (1.7 % body weight day⁻¹ for 6 g fish or 0.6 % body weight day⁻¹ for 300 g; continuous dosing), temperature (17–23 °C), light regime (16 h day⁻¹, 125 lx, industrial white light), water turnover rate (1.2× tank volume h⁻¹), and ketogenic-type diet (high protein-to-energy ratio: 20–25 g MJ⁻¹; high protein-to-carbohydrate ratio 3:1; non-protein energy dominated by lipids) are reportedly preferred feeding conditions in RAS. For non-protein energy, there is a lack of information on carbohydrate processing (e.g., net energy contribution by digestible carbohydrates, fiber tolerance, starch versus non-starch polysaccharide digestibility). High dietary lipid content seems favorable, even though percids have lean flesh. Knowledge gaps exist regarding the protein and lipid sparing effects in percids. Percid feed is currently a vacant market niche, instead, the trout, sturgeon and larval weaning feed(s) are being used by the RAS managers.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742589"},"PeriodicalIF":3.9,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling Spiroplasma eriocheiris: Morphological motility, virulence mechanisms, and host defense strategies in crustacean pathogenesis","authors":"Siyuan Zhang ,&nbsp;Jiaxin Chen ,&nbsp;Yalan Jiang ,&nbsp;Yan Li ,&nbsp;Chenbin Zhu ,&nbsp;Yujun Tang ,&nbsp;Yaqi Wang ,&nbsp;Hong Huang ,&nbsp;Keming Zhong ,&nbsp;Yingrui Xiong ,&nbsp;Jiaofeng Peng ,&nbsp;Peng Liu","doi":"10.1016/j.aquaculture.2025.742580","DOIUrl":"10.1016/j.aquaculture.2025.742580","url":null,"abstract":"<div><div><em>Spiroplasma eriocheiris</em>, a helical, wall-less prokaryote belonging to the class <em>Mollicutes</em>, presents a significant threat to crustacean aquaculture. This pathogen is particularly associated with tremor disease in <em>Eriocheir sinensis</em>, the Chinese mitten crab, resulting in substantial economic losses. Despite being the first spiroplasma identified in crustaceans, our comprehension of <em>S. eriocheiris</em> pathogenesis remains incomplete. This review synthesizes current knowledge regarding <em>S. eriocheiris</em>, focusing on its distinctive morphology and motility, the contribution of virulence factors to infectivity, and the intricate interplay between pathogen evasion strategies and host immune responses. Furthermore, we discuss advancements in diagnostic and therapeutic approaches, offering perspectives for future research directions aimed at mitigating the impact of this economically important pathogen.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742580"},"PeriodicalIF":3.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143886614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ontogenetic development of larval phototaxis in Japanese amberjack, Seriola quinqueradiata and greater amberjack, Seriola dumerili","authors":"Yoshifumi Sawada ,&nbsp;Yuga Mizushima ,&nbsp;Keigo Ito ,&nbsp;Aki Miyashima ,&nbsp;Lynn Nuruki ,&nbsp;Yasuo Agawa","doi":"10.1016/j.aquaculture.2025.742555","DOIUrl":"10.1016/j.aquaculture.2025.742555","url":null,"abstract":"<div><div>The walling behavior of larvae, driven by positive phototaxis, induces jaw malformations and significantly impairs survival in <em>Seriola</em> juveniles, posing substantial challenges to their fingerling production. However, comprehensive data on the specific light intensity at which larvae exhibit phototactic behavior is currently insufficient. This information is crucial for developing effective countermeasures, such as reducing the reflected light intensity from the rearing tank wall to a level below the phototactic threshold. The current study examined the ontogenetic development of horizontal larval phototaxis in <em>Seriola quinqueradiata</em> and <em>S. dumerili</em>. Larval phototaxis was investigated by comparing the horizontal distribution of 100 individuals before and after exposure to light projected from one end of a transparent acrylic rectangular aquarium (21 × 25 × 100 cm) for a duration of 10 min, with light intensity ranging from 0.01 to 10 μmol/m²/s. The larvae of both species exhibited positive phototaxis from the commencement of feeding until approximately two weeks post-hatching at light intensities exceeding 0.10 μmol/m²/s for <em>S. quinqueradiata</em> and 0.01 μmol/m²/s for <em>S. dumerili</em>. The minimum light intensity required to elicit positive phototaxis varied ontogenetically in both species, with both shared and species-specific chracteristics. In <em>S. quinqueradiata</em> larvae, the lower threshold of positive phototaxis elevated with age, increasing from 0.10 μmol/m²/s on two days post-hatch (dph) to 10.00 μmol/m²/s by 14 dph. Positive phototaxis was not detected at 10.00 μmol/m²/s on 16 dph. <em>S. dumerili</em> larvae exhibited positive phototaxis at 3 dph under a light intensity of 0.01 μmol/m²/s, the lowest intensity assessed. This behavior was observed at all light intensities examined from 5 to 10 dph, with the exception of 7 dph. On 13 dph, they exhibited phototaxis exclusively at the minimal light intensity of 0.01 μmol/m²/s. Subsequently, the larvae did not exhibit any further positive phototaxic response. Additionally, larvae of both species exhibited negative phototaxis at the lowest light intensity examined (0.01 μmol/m²/s) at 4 dph. Furthermore, the light intensities inducing a negative phototactic response broadned with age. In <em>S. dumerili</em>, this phenomenon was exclusively observed at the minimal light intensity of 0.01 μmol/m²/s at the older age of 15 dph. The findings of this study advance the criteria for reflected light intensity from the larval rearing tank wall to mitigate larval walling behavior in both species.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742555"},"PeriodicalIF":3.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143886035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic selection for resistance to Francisella orientalis shows significant selection response in Nile tilapia","authors":"Baltasar F. Garcia ,&nbsp;Jairo A. Restrepo-Arango ,&nbsp;Marcelo S. Silva Filho ,&nbsp;Elielma L. Sousa ,&nbsp;John F.G. Agudelo ,&nbsp;Vito A. Mastrochirico-Filho ,&nbsp;Shisley C.S. Manso ,&nbsp;Carolina S. Pereira ,&nbsp;Danielle C. Dias ,&nbsp;Antonio F.G. Leonardo ,&nbsp;Fernando S. Fonseca ,&nbsp;Leonardo Tachibana ,&nbsp;Maria J.T. Ranzani-Paiva ,&nbsp;Fabiana Pilarski ,&nbsp;Diogo T. Hashimoto","doi":"10.1016/j.aquaculture.2025.742584","DOIUrl":"10.1016/j.aquaculture.2025.742584","url":null,"abstract":"<div><div>Nile tilapia (<em>Oreochromis niloticus</em>) is one of the major aquaculture species, but its production is often challenged by emerging diseases, as francisellosis caused by <em>Francisella orientalis</em>. Selective breeding for disease resistance offers a sustainable alternative to prevent outbreaks in farming facilities. This study aimed to estimate the genetic parameters and evaluate the response to selection for resistance to <em>F. orientalis</em> in Nile tilapia. We conducted two sequential challenge tests across two generations (G0 and G1) to assess the heritability (<span><math><msup><mi>h</mi><mn>2</mn></msup></math></span>) of resistance traits using pedigree information, including binary survival (BS) and time to death (TD). Additionally, six families in G1 were designated as controls, consisting of breeders with the lowest estimated breeding values (EBV). The realized response to selection (ΔG_R) was estimated using the BS trait. The expected response to selection (ΔG_E) was also estimated using the genetic parameters of the TD trait in the second challenge. Average TD results were lower in G1 than G0, 56.59 ± 17.27 and 73.70 ± 27.68 h, respectively. The <span><math><msup><mi>h</mi><mn>2</mn></msup></math></span> estimates for G0, G1 and G0 + G1 were 0.399 ± 0.07, 0.282 ± 0.08 and 0.593 ± 0.07 for TD, and 0.454 ± 0.07, 0.266 ± 0.06 and 0.478 ± 0.06 for BS. The ΔG_R at the family level were − 0.144 for G1 control, −0.0638 for G0, and 0.0122 for G1 resistance, indicating a positive effect of selection for resistance. The ΔG_E showed potential genetic gains of 33 to 109 h in TD for the next generation depending on the selection intensity. The study found that resistance to <em>F. orientalis</em> in Nile tilapia has a significant additive genetic component and selective breeding can effectively improve disease resistance, with the potential for rapid genetic gains across generations.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742584"},"PeriodicalIF":3.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The biosecurity of bivalve seed transfers during different life stages with respect to their pathogens","authors":"L.S.L. Safi ,&nbsp;E.S. McGurk ,&nbsp;R.B. Carnegie ,&nbsp;D. Bushek","doi":"10.1016/j.aquaculture.2025.742582","DOIUrl":"10.1016/j.aquaculture.2025.742582","url":null,"abstract":"<div><div>The expansion of molluscan aquaculture in the United States has induced a rising demand for molluscan seed to sustain it. Out-of-state transfers of seed from hatcheries and nurseries are considered a pathogen risk to both aquacultured and wild animals. Another obstacle to the maintenance of the industry is the small number of laboratories able to conduct molluscan health certification. We hypothesized that larger animals would have higher pathogen prevalences, as they proportionally filter more water. To investigate this, we analyzed health data of three pathogens: <em>Perkinsus</em> spp. (dermo disease) in eastern oysters (<em>Crassostrea virginica</em>), <em>Mucochytrium quahogii</em> (QPX disease) in (<em>Mercenaria mercenaria</em>), and <em>Haplosporidium nelsoni</em> (MSX disease) in both oysters and clams. Analyses included 1309 samples containing over 75,000 animals, which were categorized by life stage (gametes, larvae, seed, juveniles and adults), as well as the type of water treatment in which animals were reared. We found higher dermo prevalences (&gt; 50 %) only in larger oysters (&gt; 40 mm) that were reared in raw water. Hard clams did not present high prevalences of either dermo or QPX in any life stage, nor did MSX in oysters. There was a marked difference in prevalences between raw and treated water for all three pathogens, highlighting the importance of biosafety practices to ensure both product quality and reliable hatchery operations.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742582"},"PeriodicalIF":3.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin receptor isoform b is an essential factor for the entry of spring viremia of carp virus","authors":"Yuxia Hou,&nbsp;Yanan Liu,&nbsp;Ling Shao","doi":"10.1016/j.aquaculture.2025.742577","DOIUrl":"10.1016/j.aquaculture.2025.742577","url":null,"abstract":"<div><div>Spring viraemia of carp virus (SVCV) causes the infectious and haemorrhagic disease spring viraemia of carp in cyprinids. Receptors determine the initiation of viral infection. However, the SVCV receptor remains unknown. The envelop glycoproteins (G proteins) of rhabdoviruses play a crucial role in viral entry. In this study, using immunoprecipitation followed by mass spectrometry, insulin receptor isoform b (Insrb) was found to interact with the SVCV G protein. Immunoprecipitation and immunofluorescence assays further confirmed that Insrb interacted with the SVCV G protein at the cell membrane. SVCV infection promoted Insrb expression both <em>in vitro</em> and <em>in vivo</em>. Insrb overexpression enhanced SVCV infection, whereas Insrb knockdown by siRNAs induced a marked reduction in the entry of SVCV into host cells. Treatment with the Insrb inhibitor NVP-ADW742 or Insrb antibodies effectively blocked SVCV infection. Moreover, <em>in vivo</em> experiments revealed that NVP-ADW742 provided protection to common carp against SVCV infection. The results of this study demonstrated that Insrb is an essential factor for SVCV cellular entry. These findings not only deepen our understanding of the cell entry mechanism of SVCV but also provide a promising therapeutic target against viral infection.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742577"},"PeriodicalIF":3.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan nanoparticles encapsulating pva-pre-miR-11881 for RNA therapy against white spot syndrome virus in the Pacific white shrimp","authors":"Pun Sangchai,&nbsp;Kunlaya Somboonwiwat","doi":"10.1016/j.aquaculture.2025.742583","DOIUrl":"10.1016/j.aquaculture.2025.742583","url":null,"abstract":"<div><div>Several studies have highlighted the significant roles of microRNAs (miRNAs) in shrimp antiviral defense. The precursor miRNA (pre-miRNA) of pva-miR-11881 from Pacific white shrimp (<em>Penaeus vannamei</em>) called “pva-pre-miR-11881”, has been found to be highly effective in enhancing the shrimp immune response to white spot syndrome virus (WSSV) infection. Although pre-miRNA molecules have higher stability than miRNA, pre-miRNA still faces the risk of nuclease degradation before reaching the target cell cytoplasm. In the current work, the pva-pre-miR-11881 was encapsulated within chitosan nanoparticles called ‘pva-pre-miR-11881 CNP’. These nanoparticles, averaging 545.2 nm in size with a 43.6 mV surface charge, demonstrated optimal stability at 4 °C. Functional assays revealed that co-injection of pva-pre-miR-11881 CNPs with WSSV effectively inhibited viral gene expression, reduced viral load, modulated hemocyte programmed cell death and autophagy, and enhanced immune responses, including phenoloxidase activity. Notably, shrimp treated with pva-pre-miR-11881 CNPs showed significantly higher survival rates post-WSSV infection compared to those treated with naked pva-pre-miR-11881. Furthermore, incorporating pva-pre-miR-11881 CNPs as a feed supplement yielded a 62 % survival rate in WSSV-infected shrimp, whereas the controls did not survive the infection. These findings highlight the potential of pva-pre-miR-11881 CNPs as a promising RNA-based therapeutic against WSSV in shrimp aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742583"},"PeriodicalIF":3.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}