Aquaculture最新文献

{"title":"The effect of the si gene on glucose adaptation ability in fish","authors":"Jing Wen ,&nbsp;Cun Yin ,&nbsp;Shisheng Wang,&nbsp;Dan Jiang,&nbsp;Zhiyong Wang,&nbsp;Ming Fang","doi":"10.1016/j.aquaculture.2025.742897","DOIUrl":"10.1016/j.aquaculture.2025.742897","url":null,"abstract":"<div><div>To investigate the role of the <em>si</em> gene in the utilization of high-carbohydrate diets in fish, we generated a zebrafish (<em>Danio rerio</em>) model with <em>si</em> gene knockout using the CRISPR/Cas9 genome editing system. A homozygous F₂ mutant line with a 119 bp deletion in the <em>si</em> gene was successfully obtained. We evaluate the high-carbohydrate adaptability of zebrafish using growth traits. Under high-glucose dietary conditions, <em>si</em> knockout significantly impaired zebrafish growth. High-glucose tolerance analysis revealed that <em>si</em>⁻/⁻ mutants exhibited marked glucose utilization dysfunction. Specifically, in diets containing 45 % and 36 % glucose, the body weight of <em>si</em>^<em>−</em><em>/</em>^<em>−</em> individuals decreased by 21.9 % (45 % glucose diet) and 34.7 % (36 % glucose diet) compared to wild-type (WT) fish (<em>P &lt;</em> 0.05), respectively; body length decreased by 6.9 % and 21.1 % (<em>P &lt;</em> 0.05); and body height decreased by 9.8 % and 19.3 % (<em>P &lt;</em> 0.05). However, under a high-protein diet based on Artemia (brine shrimp), no significant differences were observed in body weight, length, or height between WT and <em>si</em>^<em>−</em><em>/</em>^<em>−</em> fish (<em>P &gt;</em> 0.05). confirming that <em>si</em> gene specifically regulates high-glucose adaptation.</div><div>Mechanistic studies demonstrated that <em>si</em> deletion led to: (1) significant intestinal folds shortening (<em>P &lt;</em> 0.0001) and structural disorganization; (2) altered expression of genes associated with glucose metabolism (GO:0006006) and insulin-like growth factor receptor signaling (GO:0048009) in the intestinal transcriptome; and (3) dysregulation of hepatic glycolysis and energy metabolism pathways. These findings reveal a novel tripartite regulatory mechanism—intestinal folds morphology–digestive absorption–metabolic regulation—through which <em>si</em> gene governs glucose utilization in fish.</div><div>This study is the first to systematically demonstrate the critical role of the <em>si</em> gene in dietary glucose utilization in zebrafish, highlighting its involvement in maintaining energy homeostasis through modulation of glucose metabolism and digestive function. These findings provide a theoretical foundation for applying gene editing technologies to improve carbohydrate utilization efficiency in aquaculture breeding programs.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742897"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Control of salmon lice through optical delousing in commercial salmonid aquaculture","authors":"Martin Worm ,&nbsp;Jan Bulla ,&nbsp;John Harald Pettersen ,&nbsp;Benedikt Frenzl","doi":"10.1016/j.aquaculture.2025.742910","DOIUrl":"10.1016/j.aquaculture.2025.742910","url":null,"abstract":"<div><div>Parasitic salmon lice are a major challenge for salmonid aquaculture in Norway. The development of resistance against most available medicines has favored the implementation of mechanical treatment alternatives, many of which have negative implications for the health and welfare of farmed fish. Optical delousing is a controlling measure that continuously reduces salmon lice in commercial sea cages. It relies on machine vision and object detection algorithms to detect salmon lice and remove them with a targeted laser pulse on freely swimming fish. Although this technology is increasingly adopted by the farming industry, an empirical investigation of its effects on treatment frequency and salmon lice abundance has so far been lacking from scientific literature. We analyze publicly available data on salmon lice abundance and treatment frequency at individual aquaculture sites complemented with information on the use of optical delousing for the entire Norwegian coast in 2023. Our results show that sites relying on optical delousing exhibit a 50 % lower weekly treatment probability. Similar differences applied for all treatment categories and persisted despite a reduced reliance on cleaner fish and in the absence of any indication of a concomitant increase in salmon lice abundance at these sites.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742910"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide methylation and transcriptome analysis in koi carp (Cyprinus carpio var. koi): Uncovering novel molecular mechanisms in skin color differentiation and pigmentation","authors":"Xiulan Shi ,&nbsp;Xiaoyu Chen ,&nbsp;Wenbin Zhu ,&nbsp;Jun Guo ,&nbsp;Kai Lin ,&nbsp;Jianjun Fu ,&nbsp;Lanmei Wang ,&nbsp;Mingkun Luo ,&nbsp;Zaijie Dong","doi":"10.1016/j.aquaculture.2025.742902","DOIUrl":"10.1016/j.aquaculture.2025.742902","url":null,"abstract":"<div><div><em>Koi</em> carp (<em>Cyprinus carpio</em> var. <em>koi</em>) are popular ornamental fish, and their complex mottling and patterning are ideal models for studying the regulatory mechanisms of pigmentation. However, there are few studies addressing the influence of epigenetics, especially DNA methylation on <em>koi</em> coloration and phenotype formation. In this study, we focused on three phenotypes of <em>Kohaku</em> offspring, namely the Rs group (all red-skinned), the Rw group (red-white-skinned) and the Ws group (all white-skinned), each of which underwent DNA methylation and transcriptome sequencing. A comprehensive analysis revealed that the genes in the differentially methylated regions and the differentially expressed genes (DEGs) were significantly enriched in pathways such as retinol metabolism and melanogenesis. The highest number of DEGs was observed in the Rs and Rw groups, while key genes involved in color differentiation, such as <em>mitf, gch</em>2 and <em>bco</em>1, were hypermethylated and regulated in the Rs group. <em>Vivo</em> experiments also showed that inhibition of methylation led to a significant increase in the expression of genes related to the retinol and pteridine pathway (e.g. <em>pnp</em>4<em>a</em>, <em>gch</em>2 and <em>bco</em>2) in the dorsal skin but not in the ventral skin. The results of <em>in situ</em> hybridization of carotenoid metabolism genes (<em>bco</em>1 and <em>bco</em>2) also suggest that DNA methylation is involved in the regulation of different color phenotypes and coloration in various parts of the body. These findings could deepen the understanding of the dynamic changes in DNA methylation that are crucial for the differentiation and variation of skin color in <em>koi</em>, thus revealing the potential influence of epigenetics on pigmentation.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742902"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of light wavelength on feeding and surface death in larval Pacific bluefin tuna (Thunnus orientalis)","authors":"Seiya Tsuzuki ,&nbsp;Yuto Ushiro ,&nbsp;Kana Motohashi ,&nbsp;Emi Miyoshi ,&nbsp;Takahiro Tsuji ,&nbsp;Yuki Taniguchi ,&nbsp;Shinichiro Hashimoto ,&nbsp;Yasunori Ishibashi","doi":"10.1016/j.aquaculture.2026.743748","DOIUrl":"10.1016/j.aquaculture.2026.743748","url":null,"abstract":"<div><div>We investigated the expression patterns of visual opsin genes in Pacific bluefin tuna (<em>Thunnus orientalis</em>; PBT) larvae at the onset of feeding and examined how light wavelength influences their swimming behavior, feeding rate, and surface-death rate. At 2 days post-hatch (dph), all opsin genes except for the green-sensitive RH2A2 were expressed, suggesting that larvae can perceive a broad range of wavelengths. 2 dph larvae were then reared in 2 L beakers under white, yellow, green, blue, or ultraviolet (UV) light-emitting diodes (LEDs). The proportion of midwater-swimming individuals and their swimming speed were significantly higher under blue, green, and white light, indicating enhanced activity, whereas these metrics were significantly lower under yellow and UV light, suggesting avoidance behavior. Feeding experiments were subsequently conducted under the same five wavelengths, as well as under combined white + UV and dark control conditions, both with and without aeration. Regardless of water agitation, feeding rates were consistently higher in the UV, white + UV, and yellow groups than in the others. Finally, the effects of light wavelength on the surface-death rate of 3-dph larvae were examined: rates were high under blue and green light but significantly lower under UV, white + UV, yellow, and dark conditions. Validation trials in 200 L tanks confirmed that the surface-death rate was significantly higher under green than white light, and significantly lower under yellow light. Overall, these findings indicate that irradiation with UV and yellow light promotes feeding in PBT larvae while inducing an avoidance response that may help prevent surface mortality.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743748"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics reveals GABA as a key mediator of salinity stress in razor clam","authors":"Geqi Gao ,&nbsp;Zihan Lin ,&nbsp;Yuting Han ,&nbsp;Mengwei Shang ,&nbsp;Jiahui Sun ,&nbsp;Donghong Niu ,&nbsp;Jiale Li","doi":"10.1016/j.aquaculture.2026.743761","DOIUrl":"10.1016/j.aquaculture.2026.743761","url":null,"abstract":"<div><div>Climate change is intensifying salinity fluctuations in intertidal zones, causing significant economic losses to bivalve aquaculture. Understanding low salinity tolerance mechanisms is therefore crucial for safeguarding aquaculture production. To explore its responses to low salinity stress, this study employed LC-MS-based metabolomics to analyze the metabolic profiles of gill tissues from two families of the razor clam (<em>Sinonovacula constricta</em>) with contrasting tolerances: a tolerant family (F28) and a sensitive family (F8). A total of 514 significantly different metabolites (SDMs) were identified between the two families. Pathway analysis revealed eleven commonly enriched metabolic pathways, including Arginine and proline metabolism, Alanine, aspartate and glutamate metabolism, Butanoate metabolism. Notably, 4-aminobutyric acid (GABA) was involved in multiple enriched pathways and showed a more pronounced decrease in F28 compared to F8, suggesting that GABA consumption serves as a key factor in promoting low salinity tolerance in <em>S. constricta.</em> Consistent with this finding, the expression of GABA related genes (<em>GAD</em> and <em>ABAT</em>) and GABA receptor genes (<em>GABA</em>_<em>Aα</em> and <em>GABA</em>_{<em>B(1b)</em>}) was significantly upregulated under low salinity stress (<em>P</em> &lt; 0.05). Further exogenous GABA supplementation experiments demonstrated that GABA significantly promoted growth, maintained heart rate stability, enhanced <em>AQP4</em> expression, increased Na⁺/K⁺-ATPase activity, and boosted ATP contents in <em>S. constricta</em> under low salinity conditions. Thereby confirming that increased GABA availability actively supports osmotic adaptation. These findings highlight GABA's crucial role as a key mediator of low salinity tolerance and support dietary GABA supplementation as a practical strategy to enhance production in <em>S. constricta</em> aquaculture under fluctuating salinity conditions.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743761"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heritable red carapace strain of Chinese mitten crab (Eriocheir sinensis) exhibits superior antioxidant capability and pH stress resistance","authors":"Min Zhang ,&nbsp;Zonglin Yang ,&nbsp;Zouping Zhang ,&nbsp;Dongdong Zhang ,&nbsp;Hui Wang ,&nbsp;Xugan Wu","doi":"10.1016/j.aquaculture.2026.743747","DOIUrl":"10.1016/j.aquaculture.2026.743747","url":null,"abstract":"<div><div>Three heritable carapace color strains (red (R), green (G), and white (W)) of Chinese mitten crab <em>Eriocheir sinensis</em> exhibit distinct culture performance, but the potential differences in their antioxidant capability and stress resistance remain poorly unknown. This study was design to evaluate antioxidant capacity, immune indices, carotenoid composition, gene expression and high pH stress resistance among these three strains. Hepatopancreas tissues were collected from 27 adult female crabs (9 randomly selected individuals per strain) for subsequent analyses. Results showed that R strain exhibited significantly higher SOD and ACP activities, as well as elevated β-carotene and zeaxanthin levels in the hepatopancreas, whereas W strain contained the highest astaxanthin levels. Transcriptomic analysis identified several candidate genes involved in carotenoid metabolism and physiological regulation: in R vs G and R vs W, <em>UGT5</em>, <em>Ikbke</em>, <em>Hcd</em>, and <em>Dhrs11</em> were upregulated while <em>ninaB</em> and <em>BCO2</em> were downregulated; in W vs G, <em>Ikbke</em> was downregulated while <em>BCO2</em>, <em>Calml4</em>, <em>Sult1a1</em>, <em>Cbr3</em>, and <em>CYP2L1</em> were upregulated. The high pH stress test revealed that R strain exhibited the highest survivability, whereas W showed the highest mortality. Mantel tests further confirmed significant associations between carapace color parameters (<em>L</em>*, <em>a</em>*, <em>b</em>*) and multiple physiological indicators, related gene expression, and survival probability under high-pH stress. Collectively, red strain demonstrates superior antioxidant capacity and stress resistance, linked to their high hepatopancreatic carotenoid content. These findings provide valuable insights in understanding the physiological and molecular differences among three carapace color strains of <em>E. sinensis</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743747"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of melanised focal changes in Atlantic salmon (Salmo salar) skeletal muscle reveals involvement of oxidative stress, inflammation, and apoptosis","authors":"Raúl Cortés ,&nbsp;Rodrigo Zuloaga ,&nbsp;Camila Godoy-Diaz ,&nbsp;Luis Urdaneta ,&nbsp;José Luis Galaz ,&nbsp;Liane Bassini ,&nbsp;Sebastián Escobar-Aguirre ,&nbsp;Juan Antonio Valdés","doi":"10.1016/j.aquaculture.2026.743775","DOIUrl":"10.1016/j.aquaculture.2026.743775","url":null,"abstract":"<div><div>Melanised focal changes (MFCs) in the skeletal muscle of Atlantic salmon (<em>Salmo salar</em>) represent an increasing quality concern in aquaculture, as they are associated with chronic inflammation, pigment accumulation, and significant economic losses. Although the histopathological features of MFCs have been extensively described, their underlying molecular mechanisms remain poorly understood. In this study, we employed an integrative approach combining transcriptomic profiling, oxidative stress evaluation, apoptosis assessment, and in vitro functional assays to elucidate the pathways involved in MFCs development in salmon flesh. RNA-seq analysis revealed 2084 upregulated transcripts in the MFC group, primarily enriched in pathways related to cytokine–cytokine receptor interaction, NOD-like receptor signaling, and C-type lectin receptor signaling. Conversely, 337 transcripts were downregulated, predominantly associated with autophagy, insulin signaling, and mTOR signaling pathways. RT-qPCR validation of selected differentially expressed transcripts (DETs), along with oxidative damage measurements and western blot analysis of caspase-cleaved proteins, confirmed elevated oxidative stress, inflammation, and apoptosis in MFC-affected muscle. To further evaluate the role of TNF-α in mediating oxidative stress in skeletal muscle, we conducted an in vitro assay using <em>S. salar</em> myotubes. Exposure of myotubes to recombinant Atlantic salmon TNF-α induced a dose-dependent increase in reactive oxygen species (ROS) production via NF-κB activation, leading to elevated transcript expression of Tumor necrosis factor receptor (<em>tnfr1</em>). Our results support a model in which MFCs arise from persistent oxidative stress and localized inflammation, culminating in chronic immune activation, apoptosis, and pigment accumulation in skeletal muscle. This study advances our understanding of the signaling pathways involved in muscle melanisation and identifies potential molecular targets for mitigation strategies in salmonid aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743775"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Minimum oral dose of genistein required to feminize sterlet (Acipenser ruthenus) evaluated based on oocyte appearance in the testes","authors":"Toshinao Ineno ,&nbsp;Taisei Nozaki ,&nbsp;Ryuhei Kinami","doi":"10.1016/j.aquaculture.2026.743739","DOIUrl":"10.1016/j.aquaculture.2026.743739","url":null,"abstract":"<div><div>Induction of gonad development into ovaries in genetic male sterlets (<em>Acipenser ruthenus</em>) has previously been achieved by dietary supplementation with 1000 μg/g genistein (GS), a phytoestrogen. The present study aimed to determine the lowest effective GS dose capable of feminizing genetic male sterlets. Genetically male sterlets at 137 days post-hatch (DPH) were divided into four groups of 30 individuals (53.9 ± 1.87 g), orally administered GS at doses of 0, 500, 800, and 1000 μg/g diet for 180 days in in the group tanks with flow-through river water, and then fed a GS-free diet for approximately 200 days prior to sampling. The results revealed that GS did not affect survival or growth rates. Intersex gonads were observed in 15/25, 22/25, and 20/24 fish in the 0, 500, 800, and 1000 μg/g dietary GS groups, respectively, and the minimum GS dose required to induce ovarian tissue development in the testis was ≤500 μg/g diet. Furthermore, a novel classification system for ovarian tissue development within the testes was introduced that consists of stages 0 to 4 based on the histological analysis results. A significant correlation was observed between GS dose and gonadal stage (ordinal logistic regression analysis: <em>p</em> &lt; 0.05, R² = 0.279), suggesting that GS increased the proportion of gonadal ovarian tissue in a dose-dependent manner. The results of this study suggest that administering an appropriate GS dosage could contribute towards the development of formulated feed that induces feminization in farmed sterlet.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743739"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146135620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnesium chloride pre-treatment reduces transport stress in the green-lipped mussel, Perna canaliculus: an integrated physiological, behavioural and metabolomic assessment","authors":"Martin C.F. Cheng ,&nbsp;Leonardo N. Zamora ,&nbsp;Norman L.C. Ragg ,&nbsp;Natalı́ J. Delorme ,&nbsp;Anthony J.R. Hickey ,&nbsp;Brendon J. Dunphy","doi":"10.1016/j.aquaculture.2026.743763","DOIUrl":"10.1016/j.aquaculture.2026.743763","url":null,"abstract":"<div><div>The New Zealand green-lipped mussel, <em>Perna canaliculus</em>, is a key aquaculture species, and improving live transport performance is vital for quality and supply. Mussels pre-treated with magnesium chloride (MgCl₂), ambient 14 °C, or chilled 4 °C seawater were assessed after a 3-day simulated out-of-water live transport at 8 °C and recovery in seawater (+1 h, 1 day, 14 days) for gill and adductor metabolomics, haemolymph biochemistry (pH, antioxidant capacity and osmolality), heart rate (HR) and relative gaping magnitude (RGM), allowing comparison of treatment-specific responses. During the live transport simulation, mussels pre-treated at 14 °C showed elevated HR, whereas 4 °C- and MgCl₂-treated mussels had lower HR on Day 1. By Day 3, HR was low (∼1 bpm) and did not differ among treatments. Compared to 14 °C- and 4 °C-pre-treated mussels, MgCl₂-pre-treated mussels showed higher RGM and 1.6–2.3-fold lower anaerobic biomarkers (e.g., succinate, alanine and strombine), indicating reduced metabolic stress and minimal anaerobic activation. After 1-h recovery, there was a significant difference between metabolic profiles of mussels from different pre-treatment groups, which was largely driven by varying levels of metabolites for anaerobic energy metabolism (e.g., succinate and strombine) for cellular stress responses and during early stages of recovery. MgCl₂-treated mussels showed increased anaerobic markers, but lower than the other groups, suggesting less energy was needed to recover from transport-induced stress. After 1-day recovery, metabolic profiles of all groups resembled control mussels, indicating successful recovery. Overall, MgCl₂ pre-treatment of mussels provided the most effective mitigation of transport stress and supported faster recovery, highlighting its potential for improving long-distance live mussel transport.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743763"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering salinity tolerance in red tilapia (Oreochromis spp.): Insights from a genome-wide association study and genomic selection","authors":"Yi-Fan Tao ,&nbsp;Ren-Shan Huang ,&nbsp;Ji-Xiang Hua ,&nbsp;Wen Wang ,&nbsp;Si-Qi Lu ,&nbsp;Mohamed Fekri Badran ,&nbsp;Wen-Jing Tao ,&nbsp;Xiao-Chen Huang ,&nbsp;Xiao-Yi Wang ,&nbsp;Ya-Lun Dong ,&nbsp;Xu-Jun Wang ,&nbsp;Jun Qiang","doi":"10.1016/j.aquaculture.2026.743778","DOIUrl":"10.1016/j.aquaculture.2026.743778","url":null,"abstract":"<div><div>As freshwater resources become increasingly scarce, aquaculture in brackish and seawater environments offers a sustainable solution for global food security. Red tilapia (<em>Oreochromis</em> spp.), a euryhaline species renowned for its rapid growth and strong salinity adaptability, has emerged as a pivotal candidate for brackish water aquaculture. Using a panel of 160 individuals exhibiting extreme phenotypes of salinity tolerance or sensitivity, we investigated the genetic basis of the salinity tolerance trait through a genome-wide association study, and explored the potential of genomic selection to advance breeding of salt-tolerant strains. We identified 257 suggestively associated and 16 significantly associated single nucleotide polymorphisms (SNPs) within a major genomic region on chromosome 18 (26.84–28.67 Mb), with <em>parp2</em> implicated as the causal gene. Functional annotation revealed 80 candidate genes associated with ion transport, lipid metabolism, and immune response. Multiplex PCR amplification validated 12 key loci, confirming the reliability of associated SNPs. Notably, <em>parp2</em> transcript levels were significantly elevated in individuals carrying advantageous genotypes under acute salinity stress, suggesting it may play a critical role in salinity tolerance. Among six genomic selection models, Bayes C had the highest predictive accuracy for the salinity tolerance trait. Our findings provide genetic insights into the salinity tolerance of red tilapia and offer a feasible approach for boosting the productivity of aquaculture in brackish water via genomic selection.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"617 ","pages":"Article 743778"},"PeriodicalIF":3.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}