Aquaculture最新文献

{"title":"Temperature-dependent shell disease may relate to bacterial community changes of mud crab Scylla paramamosain in RAS","authors":"Kaijia Yu ,&nbsp;Xiaopeng Wang ,&nbsp;Chunlin Wang ,&nbsp;Changkao Mu ,&nbsp;Yangfang Ye ,&nbsp;Pingping Zhan ,&nbsp;Ronghua Li ,&nbsp;Ce Shi","doi":"10.1016/j.aquaculture.2025.742488","DOIUrl":"10.1016/j.aquaculture.2025.742488","url":null,"abstract":"<div><div>The previous study found that recirculating aquaculture system (RAS) in which temperature could be controlled was a feasible method for <em>S. paramamosain</em> to overwinter, but shell disease occurred during the overwintering process (12 °C–20 °C). Therefore, this study explored how temperature affected the occurrence of crab shell disease and potential pathogens, providing guidance for the overwintering management of <em>S. paramamosain</em>. This study investigated the prevalence of shell disease of <em>S. paramamosain</em> and analyzed the bacterial community dynamics in water and on the carapace under different overwintering temperatures (12, 16, and 20 °C) over 8 weeks. Shell disease incidence increased with temperature (<em>P</em> &lt; 0.05), peaking at 86.7 ± 23.1 % in the 20 °C group. Lesions primarily appeared on the dorsal carapace and were characterized by melanization, structural damage, and bacterial colonization. Bacterial diversity in water remained stable across temperatures (<em>P</em> &gt; 0.05), but carapace bacterial richness and phylogenetic diversity declined significantly at 20 °C (<em>P</em> &lt; 0.05). The bacterial community structure differed across temperature groups (<em>P</em> &lt; 0.05), with the relative abundance of <em>Aquimarina</em>, particularly <em>Aquimarina penaei</em> (ZOTU 92), rising on the carapace at 20 °C (<em>P</em> &lt; 0.05). Functional prediction indicated a significant increase in chitin deacetylase activity at 20 °C (<em>P</em> &lt; 0.05), suggesting that bacterial degradation of the carapace may be enhanced. Additionally, while the bacterial co-occurrence network on the carapace at 20 °C was complex, its stability declined. The interaction of bacterial communities between water and carapace also became increasingly intricate with rising temperatures. To our knowledge, this is the first study to establish a link between shell disease and overwintering temperature in mud crabs.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742488"},"PeriodicalIF":3.9,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyploid breeding of Saccharina japonica: Harnessing aposporous reproduction","authors":"Pingping Tian ,&nbsp;Yan Li ,&nbsp;Nianchao Xue ,&nbsp;Weiwei Wang ,&nbsp;Shuxiu Chen ,&nbsp;Yanling Liu ,&nbsp;Juan Sun ,&nbsp;Guangjin Liang ,&nbsp;Juping Zhao ,&nbsp;Liang Shi ,&nbsp;Nan Zhao ,&nbsp;Xia Li ,&nbsp;Xiaojie Li ,&nbsp;Linan Zhang","doi":"10.1016/j.aquaculture.2025.742491","DOIUrl":"10.1016/j.aquaculture.2025.742491","url":null,"abstract":"<div><div>Polyploid plants generally display significant advantages over their diploid counterparts, particularly in morphology, yield, and stress resistance. Polyploid breeding represents an effective strategy for developing varieties with enhanced traits. In this study, we induced aposporous reproduction from sporophytes of brown alga <em>Saccharina japonica</em>, resulting in diploid aposporous gametophytes exhibiting varied phenotypic and genetic sex. These aposporous gametophytes, alongside normal spore-derived gametophytes, were employed to generate triploid and tetraploid kelp through a gametophyte hybridization method. The triploid kelp (Tri-D6) demonstrated significant improvements in agronomic traits, including blade length, blade width, and fresh weight compared to diploid kelp. Although triploid kelp was capable of forming sporangia, releasing zoospores, and producing gametophytes during subsequent development, the growth of gametophytes and gametogenesis were hindered, indicating that the triploid kelp was infertile. Conversely, tetraploid kelp (Tetra-Hyb) proved fertile but lacked significant agronomic advantages, aside from increased blade thickness. These results highlight the potential of triploid kelp for cultivation, suggesting that it may offer greater practical application in kelp farming. This study establishes a polyploid breeding strategy based on aposporous reproduction for kelp, providing a foundation for further theoretical exploration into aposporous gametophytes development and the cultivation of superior polyploid kelp varieties.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742491"},"PeriodicalIF":3.9,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consumer preferences for farmed live carp (Cyprinus carpio) as safe for eating","authors":"Mohammed Ziaul Hoque PhD ,&nbsp;Bea L.P.A. Steenbekkers ,&nbsp;Jinghua Xie ,&nbsp;Øystein Myrland","doi":"10.1016/j.aquaculture.2025.742466","DOIUrl":"10.1016/j.aquaculture.2025.742466","url":null,"abstract":"<div><div>Consumers' perceptions toward unethical preservations, such as adding hazardous chemicals as a preservative to prevent microbiologic spoilage and extend whole fish's shelf life, are negative. As adding hazardous chemicals to live fish is more challenging, many marketers sell live fish as safe food to restore consumers' confidence, positively affecting consumers' purchase intention. Despite such potential, little research has examined how such selling arrangements affect consumers' seafood choices. This study uses descriptive statistics, exploratory factor analysis and a rank-ordered logit model to analyze the value consumers in an emerging economy place on food safety and their preference for live fish. We find that consumers are willing to pay a higher price for live over wild fish. Those who believe that wild fish is safe are most likely to prefer live fish and are willing to pay a price premium. When buying such live fish, they tend to shop at supermarkets. Alternatively, low-income consumers prefer farmed fish but not low-educated consumers. A strong belief in food safety regarding wild fish increases the utility of live carp; however, a strong belief in the safety of live fish decreases the utility of farmed fish insignificantly, indicating supply chain bottlenecks of live fish from safe aquaculture. Of the respondents, 44.80 % eat fish several times per week, and 91 % buy fish at least once a month. The findings could be useful for segmenting the market, establishing the potential of a live fish market, and formulating an effective seafood policy.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742466"},"PeriodicalIF":3.9,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biophysical factors, environmental regulations, and profit deviations: The case of salmon farming companies","authors":"Dengjun Zhang ,&nbsp;Geir Sogn-Grundvåg ,&nbsp;Liyuan He ,&nbsp;Ragnar Tveteras ,&nbsp;Xiaofeng Xu","doi":"10.1016/j.aquaculture.2025.742495","DOIUrl":"10.1016/j.aquaculture.2025.742495","url":null,"abstract":"<div><div>The biological production process of farmed salmon is highly vulnerable due to biophysical shocks, including sea temperature changes, fish diseases (especially sea lice infection), and toxic algae. Meanwhile, environmental externalities of salmon farming trigger stringent regulations, influencing production costs and profitability. This paper first reviews studies on the economic impact of biophysical factors on salmon farming companies. Then, we evaluate the impact of biophysical factors and capacity utilization under output restriction-based regulations on the deviations of the gross profit from the industry benchmark. In particular, we examine the differences in the impacts of biophysical factors on profit deviations for small and large firms, considering their varying capacities and financial resources available to address biophysical shocks and environmental challenges. Our empirical results indicate that diseases and capacity utilization influence profit deviations but with differing impacts on small and large firms. While capacity utilization causes a positive deviation of large firms' gross profit from the industry norm, its impact is ignorable for small firms. From the empirical results, we formulate managerial and policy-relevant recommendations for the salmon farming industry to raise profitability and pursue sustainable development.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742495"},"PeriodicalIF":3.9,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental enrichment of lumpfish (Cyclopterus lumpus) during rearing increases sea lice consumption in commercial Atlantic salmon (Salmo salar) farming","authors":"Fredrik R. Staven ,&nbsp;Nina S. Iversen ,&nbsp;Peder Jansen ,&nbsp;Manuel Gesto ,&nbsp;Torstein Kristensen","doi":"10.1016/j.aquaculture.2025.742493","DOIUrl":"10.1016/j.aquaculture.2025.742493","url":null,"abstract":"<div><div>Cleaner fish such as the lumpfish (<em>Cyclopterus lumpus</em>) are used in salmon farming for controlling infestations of sea lice. Previous laboratory studies suggest that environmental enrichment (EE) can be used to habituate lumpfish before encountering live salmon and thus promote earlier onset of cleaning behaviour. This study applied EE in commercial production, and reports findings from four trials conducted from 2020 to 2022. Juvenile lumpfish were exposed to life-like salmon models for seven days at the production facility prior to deployment in salmon net pens. During this period, the incidence of physically attaching to the salmon models significantly increased. Lumpfish did not express any outward signs of stress during exposure to the models. However, alterations in serotonergic and dopaminergic activity in the telencephalon was detected. This was potentially related to adjustments for social engagement as observed in other cleaner fish species. Stomach contents (<em>n</em> = 523) were analysed on day two and day seven after deployment and revealed a significantly higher proportion of EE lumpfish with lice in their stomachs compared to control, on day two but not on day seven. Furthermore, weight loss post deployment was observed only in the control group. Finally, serotonergic activity in the telencephalon of the enriched group was found to be lower than in the control group on day seven after deployment, which could be related to a lower level of anxiety/stress. These findings suggest that EE influences brain regulation related to social interaction with indirect positive effects on lice consumption. It is concluded that EE may encourage lumpfish to begin consuming lice more quickly after deployment in salmon net pens, and that the effect is temporal.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742493"},"PeriodicalIF":3.9,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lysine deacetylase inhibitors (KDACi) enhance the adaptability of tilapia (Oreochromis niloticus) to carbonate alkalinity stress","authors":"Longsheng Zhang ,&nbsp;Yan Zhao ,&nbsp;Wenkai Xiong ,&nbsp;Menghui Cao ,&nbsp;Jinliang Zhao ,&nbsp;Jikui Wu ,&nbsp;Junling Zhang","doi":"10.1016/j.aquaculture.2025.742492","DOIUrl":"10.1016/j.aquaculture.2025.742492","url":null,"abstract":"<div><div>Enhancing the carbonate alkalinity tolerance of fish is a critical strategy to address the challenges of sustainable aquaculture in alkaline water and meet the rising demand for aquatic products. Lysine deacetylase inhibitors (KDACi) have been demonstrated to mitigate environmental stress and may enhance carbonate alkalinity tolerance in fish. This study investigated the effects of KDACi on immune function, antioxidant capacity, ion regulation, and tissue damage of Nile tilapia (<em>Oreochromis niloticus</em>) exposed to acute carbonate alkalinity stress. A total of 360 fish (7.21 ± 1.49 g) were divided into three different treatments over a 7-day period: KDACi injection (KDACi group), DMSO injection (DMSO group), and a negative control (NC group). Additionally, the carbonate alkalinity tolerance of <em>O. niloticus</em> was assessed under daily exposure to 2 g of mixed carbonate for each treatment group. The results showed that alkaline phosphatase activity in the gills of KDACi-treated <em>O. niloticus</em> was significantly higher on days 2 and 5, while aspartate kinase activity was elevated on days 2, 5 and 6. Catalase activity increased on days 2, 4, 5 and 7 (<em>P</em> &lt; 0.05), and malondialdehyde levels were significantly reduced on days 2, 3, 5, 6 and 7 (<em>P</em> &lt; 0.05). These findings suggest that KDACi enhances the immune resistance and antioxidant capacity of <em>O. niloticus</em>. <em>SLC4A4</em> expression decreased significantly on days 3, 5 and 6 (<em>P</em> &lt; 0.05), whereas <em>CA</em> and <em>Taut</em> expression levels significantly increased on days 3 and 5 (<em>P</em> &lt; 0.05). These results suggest that KDACi could counteract carbonate alkalinity stress by regulating acid-base and osmotic balance in <em>O. niloticus</em>. Histopathological and ultrastructural observations further revealed that KDACi effectively prevents and repairs damage to gill structure and mitochondria of <em>O. niloticus</em> under carbonate alkalinity stress. Notably, under increasing carbonate concentration stress, KDACi reduced <em>O. niloticus</em> mortality by 40 % after 96 h. KDACi treatment significantly reduced mortality in <em>O. niloticus</em> under carbonate alkalinity stress. These findings suggest that KDACi has potential as a beneficial dietary supplement in salt-alkaline water aquaculture, enhancing the carbonate alkalinity tolerance in <em>O. niloticus</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742492"},"PeriodicalIF":3.9,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gametogenesis and its potential role in regulating autophagy in the cephalopod, Sepiella japonica","authors":"Yifan Liu ,&nbsp;Shuang Chen ,&nbsp;Yang Chen ,&nbsp;Xinyu Zhao ,&nbsp;Qi Wang ,&nbsp;Tao Zhang ,&nbsp;Bingjian Liu ,&nbsp;Jialang Zheng ,&nbsp;Lihua Jiang ,&nbsp;Shun Chen ,&nbsp;Huilai Shi ,&nbsp;Weihua Song ,&nbsp;Xiaojun Yan","doi":"10.1016/j.aquaculture.2025.742490","DOIUrl":"10.1016/j.aquaculture.2025.742490","url":null,"abstract":"<div><div>Autophagy is a crucial process that impacts a range of physiological processes in many organisms, including the reproductive system. However, there has been limited research on whether a relationship between autophagy and gametogenesis exists in cephalopods. <em>Sepiella japonica</em> is an important species of cephalopod found in the East China Sea. In this study, we focused on morphological and histological aspects of gametogenesis in <em>S. japonica</em> and investigated the expression patterns of autophagy-related genes (<em>LC3</em> and <em>BECN1</em>) and distribution of their corresponding proteins. Similar analyses were carried out for apoptosis marker genes (<em>Cas3</em> and <em>p53</em>) and sex marker genes (<em>Sox4</em> and <em>Sox30</em> for males, <em>Nanos-1</em> and <em>FTZ-F1</em> for females). The findings indicated that the autophagy marker genes <em>LC3</em> and <em>BECN1</em> were highly conserved and showed similar expression patterns, with peak expression in the mature testis at Stage 3a (56 g) and in the developing ovary at Stage 2a (19 g). The immunofluorescence and fluorescence in situ hybridization signals of LC3 were primarily observed in spermatids, spermatozoa, and some adjacent Sertoli cells in the testis and in peripheral follicular cells of oocytes in the ovary; this finding aligned with the results of gene expression analysis. Furthermore, the expression trends of the apoptosis marker genes <em>Cas3</em> and <em>p53</em>, male marker genes <em>Sox4</em> and <em>Sox30</em>, and female marker genes <em>Nanos-1</em> and <em>FTZ-F1</em> demonstrated remarkable consistency, suggesting potential underlying relationships between them. This study provides the first evidence of the involvement of autophagy in the gametogenesis of cephalopods, which was evolutionarily conserved. Additionally, we detected potential crosstalk between autophagy and apoptosis in the gonads of cephalopods, thus establishing a foundation for further research on autophagy in germ lines of marine mollusks.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742490"},"PeriodicalIF":3.9,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance of zoonotic bacteria isolated from shrimp aquaculture environments in Selangor, Malaysia","authors":"S. Devadas ,&nbsp;Z. Zakaria ,&nbsp;M. Shariff ,&nbsp;S. Bhassu ,&nbsp;M. Karim ,&nbsp;I. Natrah","doi":"10.1016/j.aquaculture.2025.742480","DOIUrl":"10.1016/j.aquaculture.2025.742480","url":null,"abstract":"<div><div>Intensive aquaculture farming has caused an increase in antimicrobial use (AMU) and the emergence of antimicrobial resistance (AMR). Malaysia is the ninth-largest shrimp exporter in the world. Nonetheless, the available data on AMR in shrimp aquaculture is insufficient to support efforts to combat the issue effectively. This study analyses the antimicrobial susceptibility profile and multiple antibiotic resistance (MAR) indexes of <em>Vibrio parahaemolyticus, Escherichia coli</em> and <em>Salmonella</em> in shrimp aquaculture environments in Selangor State. In total, 47 bacterial isolates, consisting of 33 (70.2 %) <em>V. parahaemolyticus,</em> 12 (25.5 %) <em>E. coli</em> and two (4.3 %) <em>Salmonella,</em> were isolated. Other than the intrinsically resistant-related classes of antibiotics, the <em>V. parahaemolyticus</em> isolates showed the top five highest percentages of resistance towards ampicillin (81.8 %, <em>n</em> = 27), followed by erythromycin (69.7 %, <em>n</em> = 23), neomycin (60.6 %, <em>n</em> = 20), kanamycin (54.5 %, <em>n</em> = 18) and ciprofloxacin (39.4 %, <em>n</em> = 13). Likewise, the <em>E. coli</em> showed resistance towards ampicillin (66.7 %, <em>n</em> = 8), followed by 58.3 % (<em>n</em> = 7) to azithromycin and cephalexin and 41.7 % (<em>n</em> = 5) to tetracycline, sulfamethoxazole/trimethoprim and neomycin. Thirty (63.8 %) isolates exhibited a MAR index &gt;0.2, while 31.9 % (<em>n</em> = 15) were classified as multidrug-resistant (MDR) and demonstrated seven types of resistance patterns. The AMU and other potential sources may contribute to the presence of AMR bacteria in aquaculture. Therefore, enhanced AMU and AMR monitoring and investigation into genome characteristics are crucial to better understanding and minimizing the risk of AMR in shrimp aquaculture, particularly in Malaysia, with implications for global aquaculture practices.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742480"},"PeriodicalIF":3.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcription factor FOXD3 participates in the immune response of Pinctada fucata martensii by regulating nAChR-5","authors":"Min Yang ,&nbsp;Yongge Huang ,&nbsp;Xinyi Lin ,&nbsp;Zhihan Li ,&nbsp;Shilin Zheng ,&nbsp;Youmei Qu ,&nbsp;Yue Wen Deng ,&nbsp;Ronglian Huang ,&nbsp;Yu Jiao","doi":"10.1016/j.aquaculture.2025.742486","DOIUrl":"10.1016/j.aquaculture.2025.742486","url":null,"abstract":"<div><div><em>Pinctada fucata martensii</em>, a significant seawater pearl oyster, requires transplantation surgery for pearl production. However, transplantation surgery often induces inflammatory responses, leading to immune rejection or mortality of the pearl oysters. Our previous research elucidated the crucial role of nicotinic acetylcholine receptor 5 from <em>P. f. martensii</em> (<em>Pm</em>nAChR-5) in the transplantation immunity. In this study, we obtained the 2000 bp upstream of the coding sequences of <em>Pm</em>nAChR-5 genes and verified its highly promoter activity using the dual-luciferase reporter system. Forkhead transcription factor D3 (<em>Pm</em>FOXD3) was predicted as a key regulator of <em>Pm</em>nAChR-5. Dual luciferase reporter assay demonstrated that overexpression of <em>Pm</em>FOXD3 significantly enhanced the promoter activity of <em>Pm</em>nAChR-5. Electrophoretic mobility shift assay (EMSA) verified the binding site (<em>TTTTGTTTGTT</em>) of <em>Pm</em>FOXD3 in the promoter region of <em>Pm</em>nAChR-5. Transcriptomic analysis revealed that down-regulated expression of <em>Pm</em>FOXD3 lead to significant enrichment in pathways such as C-type lectin receptor and Ras signaling pathways, along with knockdown of pathway-related genes and decreased TNF-α levels in serum. These findings suggested that PmFOXD3 is involved in the immune response by regulating the promoter activity of <em>Pm</em>nAChR-5. Our results provide novel molecular targets for mitigating immune rejection during pearl production.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742486"},"PeriodicalIF":3.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glutathione mitigates hypoxia stress-induced liver injury in juvenile grass carp (Ctenopharyngodon idellus) by alleviating mitochondrial dysfunction and autophagy","authors":"Shenghua Zeng ,&nbsp;Xiaoqiu Zhou ,&nbsp;Lin Feng ,&nbsp;Pei Wu ,&nbsp;Yang Liu ,&nbsp;Yaobin Ma ,&nbsp;Xiaowan Jin ,&nbsp;Hongmei Ren ,&nbsp;Jun Jiang ,&nbsp;Hua Li ,&nbsp;Ruinan Zhang ,&nbsp;Weidan Jiang","doi":"10.1016/j.aquaculture.2025.742489","DOIUrl":"10.1016/j.aquaculture.2025.742489","url":null,"abstract":"<div><div>Hypoxia is a prevalent phenomenon in aquaculture, threatening the survival and growth of fish. The present study investigated the effects of dietary glutathione on hepatopancreatic injury in juvenile grass carp under hypoxia stress and its possible mechanisms. We formulated six diets with different levels of glutathione (0.00, 145.95, 291.90, 437.85, 583.80 and 729.75 mg/kg) to feed juvenile grass carp with initial body weights (11.08 ± 0.01 g) for 70 days, followed by a 96-h hypoxia stress experiment. The results of this study showed that glutathione improved the growth performance of juvenile grass carp and alleviated hypoxia-induced glutathione depletion (GSH and GCL). In addition, glutathione decreased serum GPT and GOT activities and reduced ROS, MDA and PC contents in the hepatopancreas to attenuate tissue oxidative damage. Moreover, glutathione alleviated hypoxia stress-induced hepatopancreatic mitochondrial dysfunction, which may be related to the enhancement of mitochondrial oxidative phosphorylation and improvement of the mitochondrial quality control system. Furthermore, the ability of glutathione to alleviate hypoxia-induced hepatopancreatic autophagy may be associated with the inhibition of autophagy initiation, nucleation, elongation and degradation phases. In conclusion, glutathione attenuated hypoxia stress-induced hepatopancreatic injury in fish, which may be related to the fact that glutathione attenuated mitochondrial dysfunction and autophagy. Using the SGR from growth experiment and serum GPT activity under hypoxia stress as markers, it was determined that the optimum levels of glutathione to be added to juvenile grass carp for growth and against hypoxia stress were 451.50 mg/kg and 437.88 mg/kg, respectively.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742489"},"PeriodicalIF":3.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}