Aquaculture最新文献

{"title":"Enhanced growth, antioxidant responses and resilience to Vibrio alginolyticus induced by Lactobacillus bulgaricus supplementation in Liza ramada","authors":"Hany M.R. Abdel-Latif ,&nbsp;Riad H. Khalil ,&nbsp;Ali A. Soliman ,&nbsp;Mahmoud S. Gewaily ,&nbsp;Asem A. Amer ,&nbsp;Mahmoud Dawood ,&nbsp;Md Reaz Chaklader","doi":"10.1016/j.aquaculture.2025.743163","DOIUrl":"10.1016/j.aquaculture.2025.743163","url":null,"abstract":"<div><div>This study tested five diets supplemented with <em>Lactobacillus bulgaricus</em> (LB; 0, 1, 2, 3, and 4 g/kg) (designated as CNT, LB LB1, LB2, LB3, and LB4) with the basal diet on growth, antioxidant capacity, hepatic and intestinal histoarchitecture, and resistance of <em>Liza ramada</em> to <em>Vibrio alginolyticus</em> infection. Fish (8.47 ± 0.40 g) were stocked in triplicate groups in hapas fixed in cement ponds and fed on freshly prepared test diets for 60 days. Fish-fed LB-supplemented diets demonstrated higher final weight (FBW), and specific growth rate (SGR) compared to the CNT group (ANOVA <em>P</em> &lt; 0.0001), with the cubic trend for FBW and quadratic trend for SGR, as analyzed by the polynomial model. Similarly, higher feed intake and feed conversion ratio were found in groups fed LB-supplemented diets with a strong cubic trend with increasing dietary LB levels. The hepatic SOD (superoxide dismutase) and CAT (catalase) enzyme activities improved significantly in all LB groups while a significant increase in GPx (glutathione peroxidase) enzyme activity and decrease in MDA (malondialdehyde) levels were found in all dietary groups, except LB1 when compared to those fed CNT diet. The hepatic antioxidant responses were well fit with all polynomial models, but the cubic fit was highly significant in response to dietary LB levels. The intestinal and hepatic histoarchitecture showed normal and healthy structures across all treatments. The fish survival rate (55–70 %) improved significantly in fish fed 2–4 g/kg LB compared to the CNT group (15 %). To conclude, a dietary dose of 2–4 g/kg can be supplemented to bolster the infection resistance against pathogenic bacteria by enhancing growth, feed utilization, and antioxidant responses, potentiating its application as a functional additive to maximize the profitability of mullet farming.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743163"},"PeriodicalIF":3.9,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a dorsal fin cell line from largemouth bass (Micropterus salmoides) and its apoptosis induced by MSRV","authors":"Jianfei Lu ,&nbsp;Hongyang Xu ,&nbsp;Sheng Luo ,&nbsp;Jiahui Liang ,&nbsp;Hao Wang ,&nbsp;Jiong Chen","doi":"10.1016/j.aquaculture.2025.743164","DOIUrl":"10.1016/j.aquaculture.2025.743164","url":null,"abstract":"<div><div>The largemouth bass (<em>Micropterus salmoides</em>), also known as the California bass, is an important freshwater aquaculture species worldwide. <em>Micropterus salmoides</em> rhabdovirus (MSRV) is a major pathogen threatening the sustainable development of largemouth bass aquaculture. Here, we established a novel cell line, named <em>Micropterus salmoides</em> dorsal fin (MSDF) cell line, which supports MSRV propagation and enables investigation of virus-induced apoptosis. Transcriptome analysis showed that MSRV infection altered host gene expression, upregulating 313 genes and downregulating 126 genes, which were functionally enriched in immune/metabolic pathways, including the p53 signaling pathway, apoptosis, peroxisome, lysosome pathways, and nucleotide metabolism. Furthermore, DAPI and TUNEL staining confirmed that MSRV infection induced apoptosis in MSDF cells. Notably, inhibiting cell apoptosis suppressed viral replication, and mitochondrial dysfunction was implicated in MSRV-triggered apoptosis. Collectively, we developed an MSRV-susceptible cell line (MSDF), which serves as an ideal platform for characterizing MSRV pathogenesis, particularly its apoptotic mechanisms.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743164"},"PeriodicalIF":3.9,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The SPHK/S1P/S1PR axis promotes LPS challenge- and Edwardsiella piscicida infection-induced proinflammatory immune responses and bacterial killing activity in the head kidney macrophages of the Japanese flounder Paralichthys olivaceus","authors":"Shuo Li,&nbsp;Yao Zhang,&nbsp;Yue Cao,&nbsp;Jinsheng Sun","doi":"10.1016/j.aquaculture.2025.743158","DOIUrl":"10.1016/j.aquaculture.2025.743158","url":null,"abstract":"<div><div>Sphingosine kinases (SPHK1 and SPHK2) are a family of isozymes that phosphorylate sphingosine to generate the bioactive sphingolipid metabolite sphingosine-1-phosphate (S1P), which subsequently binds to and activates S1P receptors (S1PRs). Although the SPHK/S1P/S1PR axis has been demonstrated to play crucial roles in modulating immune and inflammatory responses in mammals, its role in teleost immunity remains largely unexplored. In this study, we characterize and investigate the regulatory roles of two <em>sphk</em> isoforms, <em>sphk1-like</em> (<em>sphk1-L</em>) and <em>sphk2</em>, in the inflammatory response in head kidney macrophages (HKMs) from the Japanese flounder <em>Paralichthys olivaceus</em>. SPHK1-L and SPHK2 proteins display conserved domain organizations with their mammalian homologs, highlighting evolutionary conservation across vertebrate lineages. Gene expression analysis reveals that <em>sphk1-L</em> and <em>sphk2</em> are highly induced in HKMs by LPS stimulation and <em>Edwardsiella piscicida</em> infection, suggesting their involvement in fish immune response. Pharmacological inhibition of SPHKs enzymatic activity or siRNA knockdown of either <em>sphk1-L</em> or <em>sphk2</em> expression markedly attenuated LPS- and <em>E. piscicida</em>-induced expression of proinflammatory cytokines, chemokines, and inflammasome-related genes in HKMs. Conversely, treatment with the sphingosine analog FTY720 potentiated the expression of inflammatory immune response genes in LPS-treated and <em>E. piscicida</em>-infected HKMs, but this effect was significantly attenuated when SPHK enzymatic activity was pharmacologically inhibited, indicating that SPHK catalytic activity is essential for the enhanced expression of inflammatory response genes in fish macrophages. Additionally, we show that exogenous S1P treatment significantly enhances proinflammatory gene expression in LPS-stimulated and <em>E. piscicida</em>-infected HKMs, and multiple <em>S1PRs</em> are expressed in HKMs, suggesting that S1PR activation by extracellular S1P may involve in this process. Moreover, we demonstrate that SPHK inhibition facilitates the proliferation of intracellular bacteria <em>E. piscicida,</em> while S1P or FTY720 treatment markedly reduced the bacterial colonization in flounder HKMs. Collectively, our studies reveal that the SPHK/S1P axis promotes LPS challenge- and bacterial infection-induced proinflammatory immune responses and antibacterial activity in teleost fish macrophages, likely through the activation of multiple S1PRs.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743158"},"PeriodicalIF":3.9,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transition from clear-water to biofloc systems improves growth, antioxidant response, and gene expression in Penaeus vannamei","authors":"Yanshuo Guo ,&nbsp;Wenchang Liu ,&nbsp;Yanjun Yu ,&nbsp;Guozhi Luo ,&nbsp;Hongxin Tan","doi":"10.1016/j.aquaculture.2025.743161","DOIUrl":"10.1016/j.aquaculture.2025.743161","url":null,"abstract":"<div><div>Integrated aquaculture systems that combine biofloc technology (BFT) with recirculating clear-water (CW) environments have attracted increasing interest. This study investigated the effects of transitioning from a CW to a BFT system on culture performance of <em>Penaeus vannamei</em>. Two culture models were tested: a single CW system (SCW) and a CW to BFT transition system (TBI). Over the 50-day experiment, TBI maintained lower ammonia and nitrite concentrations than SCW. Gill histology revealed no overt necrosis or structural damage in either treatment group. TBI achieved a higher yield (2.79 kg/m³) and survival (96.5 %) than SCW (<em>P</em> &lt; 0.05). The results of antioxidant enzyme analysis indicated improved oxidative status in TBI, and lower malondialdehyde (MDA) levels relative to SCW (<em>P</em> &lt; 0.05). Transcriptome analysis showed that bioflocs may produce some “stimulation” to shrimp, which increases immunity and improves adaptability to the environment. Additionally, Energy metabolism also appeared elevated in TBI, reflected by higher Na⁺-K⁺-ATPase and total ATPase activities and the upregulation of ATP-linked and glycogen-biosynthesis genes. However, the total suspended solids (TSS) exceeded 600 mg/L late in culture period, which may have influenced physiological responses. Overall, the CW to BFT transition was associated with enhanced stress resistance, immune status, and energy metabolism, as well as improved growth performance and culture efficiency.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743161"},"PeriodicalIF":3.9,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of a novel type (Insulin-type) of insulin-like peptide involved in glucose metabolism and growth in Pacific white shrimp Litopenaeus vannamei","authors":"Jiawei Liu ,&nbsp;Zijian Liu ,&nbsp;Zijie Liu ,&nbsp;Xiaowei Song ,&nbsp;Su Liu ,&nbsp;Lin Song ,&nbsp;Yi Gao","doi":"10.1016/j.aquaculture.2025.743162","DOIUrl":"10.1016/j.aquaculture.2025.743162","url":null,"abstract":"<div><div>Insulin-like peptides (ILPs) are a family of highly conserved peptide hormones that play fundamental regulatory roles in growth, metabolism, and reproduction across metazoans. Although ILPs have been extensively studied in vertebrates and insects, their characterization in crustaceans, particularly their specific roles in regulating shrimp growth and glucose homeostasis, remain largely unknown. In this study, we report the identification and functional characterization of a novel Insulin-type ILP gene, designated <em>LvIns</em>, from the Pacific white shrimp (<em>Litopenaeus vannamei</em>). The full-length <em>LvIns</em> cDNA is 676 bp and sequence analysis indicated that <em>LvIns</em> possesses the typical conserved sequences and structural features of ILPs. Tissue expression profiling demonstrated that <em>LvIns</em> is mainly expressed in female reproductive tissues and nerve tissue. In vivo and in vitro experiments involving glucose treatment and exogenous insulin treatment confirmed that <em>LvIns</em> participates in the regulation of glucose metabolism in shrimp. Furthermore, RNA interference experiments (including long-term and short-term interference) demonstrated that knockdown of <em>LvIns</em> expression significantly reduced the growth rate of shrimp and decreased serum glucose clearance efficiency. Further research indicated that that <em>LvIns</em> may influence shrimp growth by regulating the downstream PI3K/AKT signaling pathways, thereby modulating key glucose metabolic processes such as glycolysis and gluconeogenesis. This study provides a comprehensive characterization of an Insulin-type ILP in a crustacean species, elucidating its crucial role in regulating growth and glucose metabolism.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743162"},"PeriodicalIF":3.9,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In–situ aquaculture treatment: Co–addition of autotrophic and heterotrophic bacteria with biodegradable polymers (PHBV) to Penaeus vannamei culture systems","authors":"Kai Luo ,&nbsp;Liang Cui ,&nbsp;Mingyang Wang ,&nbsp;Yumeng Xie ,&nbsp;Mingjing Li ,&nbsp;Yunhui Liu ,&nbsp;Tengfei Li ,&nbsp;Ruiyong Fan ,&nbsp;Xiangli Tian","doi":"10.1016/j.aquaculture.2025.743159","DOIUrl":"10.1016/j.aquaculture.2025.743159","url":null,"abstract":"<div><div>As a novel trend, biodegradable polymers, specifically poly(3–hydroxybutyrate–co–3–hydroxyvalerate) (PHBV), are being utilized as carbon source and biofilm carrier in the in–situ aquaculture systems to remove nitrogen from wastewater. This study investigated the differential responses to various aquaculture systems with the addition of autotrophic bacteria and PHBV (PNB group), heterotrophic bacteria and PHBV (PBS group), autotrophic and heterotrophic bacteria, along with PHBV (PBN group), single autotrophic bacteria (NB group), as well as clear water system (the control, CN group), on the growth, immune enzyme activity, water quality, and microbiota in rearing water, shrimp intestine, carbon source in <em>Penaeus vannamei</em> culture systems. Results showed that these culture systems were more effective in improving water quality than clear water system, with PBN group exhibited the best performance. The growth and immune enzyme activity of shrimp in these culture systems were significantly enhanced than those in clear water system. Although microbial diversity in rearing water, shrimp intestine, and carbon source was not significantly altered, differences in microbial community richness were observed in rearing water and carbon source. Significant differences in microbial community structure were noted in rearing water, but no such differences were observed in shrimp intestine or carbon source. Total ammonia nitrogen, total nitrogen, and nitrite nitrogen were identified as primary drivers of microbial community changes in rearing water, shrimp intestine, and carbon source, respectively. Furthermore, these culture systems significantly altered keystone nodes in microbial networks, with PBS group improving the stability of microbial networks in both rearing water and shrimp intestine. The addition of autotrophic and heterotrophic bacteria, along with PHBV in system increased the proportion of nitrogen cycling functions within microbial communities in rearing water and carbon source. Additionally, the abundance of potential pathogens in shrimp intestinal microbial communities decreased to varying degrees in these culture systems, particularly in NB group. The PBN, PBS and PNB groups significantly altered microbial community composition of the habitats in rearing water, shrimp intestine, and carbon source compared to clear water system. Notably, shrimp intestinal microbial communities were more similar to carbon source than to those in rearing water. This study evaluated the feasibility and advantages of using PHBV as carbon source and biofilm carrier in various aquaculture systems, thereby expanding the application prospects of biodegradable polymers in aquaculture practices.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743159"},"PeriodicalIF":3.9,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of sex determination genes in the Coho salmon (O. kisutch) genome and development of a rapid diagnostic test using CRISPR/Cas12","authors":"V. Martinez ,&nbsp;C. Mondaca ,&nbsp;J. Dorner ,&nbsp;P. Zamorano ,&nbsp;J. Palomino ,&nbsp;N. Galarce","doi":"10.1016/j.aquaculture.2025.743150","DOIUrl":"10.1016/j.aquaculture.2025.743150","url":null,"abstract":"<div><div>Sex determination in Coho salmon (<em>Oncorhynchus kisutch</em>) is an essential issue for aquaculture and ecological assessment but is challenging due to late-emerging sexual dimorphism. This study tackles the need for early, non-lethal sex identification by combining genomic analysis with an innovative CRISPR diagnostic tool. Using genome-wide association studies (GWAS) and whole-genome sequencing (WGS) across diverse populations, we validated the male-specific indel at the pseudogene GH2 on chromosome 10. Following Mendelian segregation of heterozygous males and homozygous females, we identified a novel sex-associated locus on chromosome 30 linked to E3 ubiquitin ligases. Strikingly, the SNPs in this gene follow the same segregation pattern of the deletion in chromosome 10, suggesting a founding event following a speciation event that occurred in <em>O. kisutch</em>. Another possible explanation is the ancestral tetraploidy of the genera, but in this case, both regions do not appear to be paralogous. This pattern has been observed in other members of the Salmonidae family. The transcriptome analysis of immature gonads revealed sex-specific gene expression in several genes on chromosome 30, but little overlap with the GWAS. We developed a CRISPR/Cas12-based diagnostic test targeting the GH2 indel, achieving 100 % accuracy in sex determination with a detection limit of 0.1 ng/μL of DNA. Requiring minimal equipment, this field-ready assay outperforms traditional methods like high-resolution melting (HRM) analysis in speed and portability. Our results will help deepen the understanding of sex determination in salmonids and provide a practical, scalable tool for aquaculture and ecological applications.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743150"},"PeriodicalIF":3.9,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a single-chain variable fragment (scFv)-conjugated affinity silk filter for the efficient removal of Ichthyophthirius multifiliis","authors":"Harsha Prakash ,&nbsp;Mitsuru Sato ,&nbsp;Katsura Kojima ,&nbsp;Chisato Sakuma ,&nbsp;Akhil Kizhakkumpat ,&nbsp;Takahiro Nagasawa ,&nbsp;Miki Nakao ,&nbsp;Tomonori Somamoto","doi":"10.1016/j.aquaculture.2025.743141","DOIUrl":"10.1016/j.aquaculture.2025.743141","url":null,"abstract":"<div><div><em>Ichthyophthirius multifiliis</em> (<em>I. multifiliis</em>) is known to infect a wide range of freshwater teleosts worldwide and elicit white spot disease (WSD). Since WSD causes serious economic loss in global aquaculture, effective means of controlling WSD are urgently needed. In this current study, we have tried to develop the pilot model of an aquarium filter that can effectively filter out the endoparasite <em>I. multifiliis</em> using “anti- <em>I. multifiliis</em> affinity silk”. This affinity silk consists of the silk fibroin L-chain (FibL) and the single-chain variable fragment (scFv) from the anti- <em>I. multifiliis</em> monoclonal antibody (mAb). This affinity silk-coated glass wool was inserted into a 5 mL syringe, which was used to filter the aquarium water with <em>I. multifiliis.</em> The filter was washed twice after the filtration, and the number of parasites in the filtrate was calculated each time. To evaluate the number of parasites captured by the filter, the number of parasites in the first filtrate and the washes were compared with the number of parasites passed through the filter initially. The number of <em>I. multifiliis</em> captured by the glass wool filter coated with anti- <em>I. multifiliis</em> affinity silk was significantly higher than the control filters, indicating that the filter is effective against highly motile and large microorganism. These results show that anti- <em>I. multifiliis</em> affinity silk can be a great tool for preventing ichthyophthiriasis, the parasitic disease caused by <em>I. multifiliis.</em></div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743141"},"PeriodicalIF":3.9,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated biochemical and transcriptomic analyses elucidate the physiological mechanisms in rainbow trout (Oncorhynchus mykiss) head kidney under hypoxia stress","authors":"Zhijia Guo ,&nbsp;Jinqiang Huang ,&nbsp;Yongjuan Li ,&nbsp;Lu Zhao ,&nbsp;Qi Wang ,&nbsp;Shenji Wu ,&nbsp;Fengqi Zhou ,&nbsp;Wenlong Jiao","doi":"10.1016/j.aquaculture.2025.743130","DOIUrl":"10.1016/j.aquaculture.2025.743130","url":null,"abstract":"<div><div>Hypoxia has emerged as a critical threat to the stability of aquatic ecosystems and the sustainability of aquaculture industries. Rainbow trout (<em>Oncorhynchus mykiss</em>), a globally significant economic fish species, exhibits remarkable sensitivity to hypoxia. However, the physiological mechanisms of head kidney in rainbow trout under hypoxia stress, especially under acute and severe hypoxia conditions, remain largely unexplored. This research explored the transcriptomic responses of rainbow trout head kidney to moderate hypoxia (4.5 ± 0.1 mg/L) and severe hypoxia (3.0 ± 0.1 mg/L) for 12 h, alongside dynamic changes in biochemical parameters during hypoxia exposure (0, 4, 8, 12, 24 h, and 1 month) and reoxygenation (R12 h and R24 h). The results showed that compared with the control group, the activities of T-AOC, CAT, T-SOD, GOT, GPT, and ACP were decreased under moderate and severe hypoxia conditions, while the content of MDA and the activity of AKP were increased. In RNA-seq analysis, we identified several hypoxia-related, immune-related and metabolism-related genes (<em>hif-1α, fih1, vhl, ddit4, egln1/2/3, cxcr1/3/4/5, ccr5/7, clec4e/m, tlr2, socs2/3, ighv3–43, ighv3–53, g6pc3, pk, ldha, hk2,</em> and <em>foxo1a</em>) from 20,399 significantly differentially expressed genes, which were enriched in insulin signaling pathway, glycolysis/gluconeogenesis, FcγR-mediated phagocytosis, hematopoietic cell lineage and B cell receptor signaling pathway. Additionally, the expression levels of <em>cxcr1, ddit4, egln2, egln3, fih1</em> and <em>foxo1a</em> in rainbow trout were significantly up-regulated under hypoxia stress and returned to normal after reoxygenation. These findings provide new insights into the physiological and molecular responses of rainbow trout under hypoxia stress and contribute to developing effective strategies for fish to cope with environmental hypoxia.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743130"},"PeriodicalIF":3.9,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat stress remediation by dietary selenium nanoparticle supplementation in Nile tilapia, Oreochromis niloticus, reared in brackish water","authors":"Morteza Yousefi ,&nbsp;Hossein Adineh ,&nbsp;Zahra Koohestani ,&nbsp;Zohreh Fazelan ,&nbsp;Sanjay Kumar Gupta ,&nbsp;Heba H. Mahboub ,&nbsp;Seyyed Morteza Hoseini","doi":"10.1016/j.aquaculture.2025.743146","DOIUrl":"10.1016/j.aquaculture.2025.743146","url":null,"abstract":"<div><div>Climate change poses significant challenges to the expansion of aquaculture production. Environmental stressors such as rising water temperatures, increased incidence of heat waves, and water salinization limit the growth of this industry. Identifying practical strategies to mitigate such effects is crucial for enhancing the sustainability of aquaculture. The present study aimed to evaluate the potential benefits of dietary selenium supplementation in Nile tilapia, <em>Oreochromis niloticus</em>, reared in brackish water for nine weeks, followed by a four-day heat stress challenge. Fish were fed diets containing 0 (control), 0.5, 1, and 2 mg/kg selenium nanoparticles in triplicate groups during nine weeks of rearing. Results indicated that dietary selenium significantly improved growth performance, feed efficiency, survival rates, and intestinal activities of digestive enzymes. Dietary selenium also improved various antioxidant and immunological parameters in plasma, intestine, and liver. Selenium supplementation boosted hepatic glutathione peroxidase, reduced glutathione, and total antioxidant capacity, before and after heat stress. Dietary selenium effectively mitigated stress-induced increases in plasma glucose, alanine aminotransferase, aspartate aminotransferase, and hepatic malondialdehyde. While heat stress elevated plasma cortisol and decreased plasma T₃ and T₄ levels and hepatic superoxide dismutase activity, selenium supplementation counteracted these adverse effects. In conclusion, these findings suggest that selenium supplementation, particularly at 1 mg/kg, can enhance the sustainability of Nile tilapia production under climate change. This approach improves feed efficiency, health, disease resistance, and fish survival, thereby supporting economic viability and sustainability of the aquaculture industry.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743146"},"PeriodicalIF":3.9,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}