Aquaculture最新文献

{"title":"Magnetic resonance imaging for rapid, noninvasive and accurate assessment of ovarian maturation in live Channa argus (Cantor, 1842)","authors":"Wenqian Sun ,&nbsp;Weiyin Vivian Liu ,&nbsp;Xingyu Chen ,&nbsp;Guangcan Lin ,&nbsp;Hao Li ,&nbsp;Yanting Song ,&nbsp;Chengdong Peng ,&nbsp;Wen Xiong ,&nbsp;Yu Peng ,&nbsp;Shangong Wu ,&nbsp;Shouqi Xie ,&nbsp;Sovan Lek ,&nbsp;Mette Sørensen ,&nbsp;Haokun Liu ,&nbsp;Dong Liu ,&nbsp;Lei Pan","doi":"10.1016/j.aquaculture.2025.742872","DOIUrl":"10.1016/j.aquaculture.2025.742872","url":null,"abstract":"<div><div>Current methods for the determination of ovarian maturation in fish have shortcomings. They are often time-consuming, inaccurate, and fish are sacrificed during the procedure. A novel noninvasive and accurate method to assess ovarian maturation can be to use magnetic resonance imaging (MRI). Long scanning time is a limitation for use in fish. A clinical MRI with a new sequence employing a shorter scanning time may be more feasible for the assessment of ovarian maturation in female fish. The aim of this study was to investigate ovarian maturation in <em>Channa argus</em> by clinical MRI with the three-dimensional fast spin echo Cube-flex (3D-FSE-Cube-Flex) T₂WI sequence, compared with two traditional sequences. The results demonstrated that compared with two traditional sequences, the 3D-FSE-Cube-Flex T₂WI sequence greatly reduced scanning time (only requiring 140 s per fish) without reducing the scanning quality. A significant correlation (R² = 0.985, <em>p</em> &lt; 0.001, <em>N</em> = 112) was found between the percentage volume of the ovary measured by MRI and the gonadosomatic index (GSI). Besides, ovarian maturation MRI images at different development stages were captured. These findings suggest that clinical MRI with the 3D-FSE-Cube-Flex T₂WI sequence is a rapid, accurate, and noninvasive method for assessing ovarian maturation, with great potential applications in sustainable aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742872"},"PeriodicalIF":3.9,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermented fish meal derived from barramundi by-product enhances growth, immunity, and gut microbiota in white shrimp Penaeus vannamei","authors":"Ann-Chang Cheng ,&nbsp;Rui-Jun Kang ,&nbsp;Chun-Hung Liu","doi":"10.1016/j.aquaculture.2025.742876","DOIUrl":"10.1016/j.aquaculture.2025.742876","url":null,"abstract":"<div><div>This study investigated the effects of barramundi, <em>Lates calcarifer</em>, by-product fish meals on the growth, immunity, and gut microbiota of white shrimp, <em>Penaeus vannamei</em>. Three variants were assessed: untreated fish meal (BFM), enzymatically hydrolyzed fish meal (EBFM), and fermented fish meal (FBFM), alongside a commercial fish meal control. Compared to commercial fish meal, barramundi by-product meals exhibited lower crude protein but higher lipid and ash contents. Fermentation with <em>Lactobacillus plantarum</em> 7–40 notably increased methionine levels in FBFM. Shrimp fed diets containing EBFM or FBFM showed significantly greater weight gain, feed efficiency, and biomass production, with FBFM yielding the most pronounced improvements. FBFM supplementation also enhanced immune parameters, including phenoloxidase, respiratory burst, superoxide dismutase, lysozyme, and phagocytic activities, alongside upregulation of immune-related genes. Post-challenge survival against <em>Vibrio alginolyticus</em> was highest in the FBFM group. Gut microbiota analysis revealed reduced <em>Vibrio</em> abundance and increased beneficial genera (e.g., <em>Enterococcus</em>, <em>Rubritalea</em>) in FBFM-fed shrimp. Principal component analysis indicated distinct microbial profiles among treatments. These results highlight FBFM as a viable alternative protein source that improves shrimp growth, immunity, gut health, and disease resistance, supporting sustainable aquafeed development within a circular economy framework.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742876"},"PeriodicalIF":3.9,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144470577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive evaluation and analysis of cold tolerance in Neopyropia yezoensis strains based on multiple traits","authors":"Zi-Xiang Zhai ,&nbsp;Guo-Ying Du ,&nbsp;Lan Wu,&nbsp;Lin Tian,&nbsp;Li-Hong Ma","doi":"10.1016/j.aquaculture.2025.742875","DOIUrl":"10.1016/j.aquaculture.2025.742875","url":null,"abstract":"<div><div><em>Neopyropia yezoensis</em>, an important economic macroalga, is cultivated in the coastal regions of Northeast Asia, where the farming season spans the cold winter. The low temperatures during this period severely impacting its growth and yield. Given these challenges, selecting high-quality strains with enhanced cold tolerance is essential for improving both yield and quality. To achieve this, it is crucial to establish a set of evaluation criteria and methods to effectively screening for cold-tolerant germplasm. In this study, the cold tolerance of 60 <em>N. yezoensis</em> strains was evaluated by assessing 13 traits, including growth rate, photosynthetic parameters, and pigment contents. Under low temperature, most traits were depressed, with the daily growth rate (DGR) being particularly affected. Principal Component Analysis (PCA) and weight analysis identified thalli area, DGR, chlorophyll <em>a</em> (Chl <em>a</em>), phycoerythrin (PE), allophycocyanin (APC) and photochemical efficiency (Φ_PSII) as primary contributors to cold tolerance. A comprehensive index (D) was developed based on the membership function and weight of each trait to rank the 60 strains into five cold tolerance levels. Over 65.0 % of strains exhibited a certain extent of cold tolerance, which aligning with the nature <em>N. yezoensis</em> as cold-water species. The optimal evaluation model was established as D = 0.387a rea + 0.122Chl <em>a</em> + 0.242F_v/fm + 0.066APC - 0.086, with R² of 0.955 (where F_v/fm represents the maximum photochemical efficiency). This research establishes a comprehensive evaluation system for cold tolerance in <em>N. yezoensis</em>, enabling the rapid screening of cold-tolerant strains and enhancing breeding efforts in the industry.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742875"},"PeriodicalIF":3.9,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing PoSAP, a serum amyloid P component in Japanese flounder (Paralichthys olivaceus) for superior antibacterial immunity in teleost fish","authors":"Sheng-han Gao ,&nbsp;Jia-qi Sun ,&nbsp;Kun-yu Zhao ,&nbsp;Gang Li ,&nbsp;Jian Zhang ,&nbsp;Xue-peng Li","doi":"10.1016/j.aquaculture.2025.742859","DOIUrl":"10.1016/j.aquaculture.2025.742859","url":null,"abstract":"<div><div>Serum amyloid P component (SAP), a member of the short pentraxin family, plays a multifaceted role in immune responses. In this study, we investigated the antibacterial immunity of SAP in <em>Paralichthys olivaceus</em> (PoSAP). PoSAP comprises 215 amino acids and shares 55.6–76.1% overall sequence identity with other fish species. <em>In vitro</em>, recombinant PoSAP (rPoSAP) showed differential binding ability to eight bacterial species and agglutinated <em>S. aureus</em> and <em>P. fluorescens</em>. rPoSAP demonstrated bacteriostatic activity against seven bacterial species and bacterial activity against <em>E. tarda.</em> In serum, the addition of rPoSAP or blocking endogenous PoSAP significantly regulated hemolytic and bactericidal activity, potentially resulting from the interaction of PoSAP with complement C1qA and C1qC chains, thereby inducing activation of the classical complement pathway. Regarding leukocyte vitality, rPoSAP bound to peripheral blood leukocytes and activated their chemotaxis, proliferation, inflammatory gene expression, phagocytosis, and reactive oxygen species production, leading to leukocyte-mediated antibacterial effects. Besides, neutrophils stimulated with rPoSAP extruded neutrophil extracellular traps. <em>In vivo</em>, <em>PoSAP</em> expression was significantly regulated by bacterial infection, and rPoSAP enhanced antibacterial effects in flounder tissues. In summary, these observations provide novel insights into the superior antibacterial immunity conferred by PoSAP in teleost fish.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742859"},"PeriodicalIF":3.9,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of climate change on mariculture in coastal China: Spatial reconfiguration and structural adaptation","authors":"Bin Yuan ,&nbsp;Jinchai Tu ,&nbsp;Zhijun Li","doi":"10.1016/j.aquaculture.2025.742874","DOIUrl":"10.1016/j.aquaculture.2025.742874","url":null,"abstract":"<div><div>As a pivotal component of marine fisheries, mariculture is confronting significant and multifaceted impacts from climate change. Understanding the climate change effects on fishermen's mariculture practices is crucial for safeguarding fishermen's livelihoods and ensuring global food security. Drawing upon panel data from 15 coastal cities in China during 2003–2022, we conducted a comprehensive analysis of mariculture spatial distribution patterns, subsequently developing a decision-making model for fishermen's mariculture to systematically assess climate change impacts. The study found that: (1) Climate change exacerbation will progressively diminish the marginal productivity of mariculture, depress fishermen's anticipated income levels, and consequently trigger a contraction in regional mariculture acreage. (2) The impacts of climate change show obvious species differences. Unfavorable climatic conditions, such as rising sea surface temperature and decreasing seawater pH, will further reduce the area of fish and shellfish mariculture, lowering the proportion of fish mariculture, thus inducing the fishermen to adjust their mariculture structure in order to avoid potential climate losses. (3) Additional analysis indicates significant heterogeneity in climate change vulnerability across production scales. The detrimental effects are more acute for large-scale mariculture enterprises in terms of area reduction, while rising sea temperatures markedly stimulate small-scale fishermen's transition toward shellfish mariculture. Therefore, it is necessary to enhance the climate early warning mechanism, tackle short-term climate changes, upgrade mariculture technology, optimize the structure of aquaculture species, increase policy support, and formulate differentiated subsidy strategies, so as to provide a reference for fishermen to adapt to climate change and stabilize their livelihoods.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742874"},"PeriodicalIF":3.9,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgae-derived feed additives improve physiological health, intestinal integrity, and welfare in juvenile gilthead seabream (Sparus aurata) fed plant-based diets","authors":"Anyell Caderno ,&nbsp;Milagrosa Oliva ,&nbsp;Isabel Barranco ,&nbsp;Antonio Astola ,&nbsp;Juan Fuentes ,&nbsp;Francisco Javier Alarcón-López ,&nbsp;Juan Miguel Mancera ,&nbsp;Juan Antonio Martos-Sitcha","doi":"10.1016/j.aquaculture.2025.742873","DOIUrl":"10.1016/j.aquaculture.2025.742873","url":null,"abstract":"<div><div>Healthy aquaculture diets, which contain beneficial ingredients for the organism, are an emerging option. Within this framework, dietary additives play a crucial role, providing numerous advantages to animal health and physiological status. The present study aimed to assess how the growth and physiological health of gilthead seabream (<em>Sparus aurata</em>), a carnivorous fish species, are affected by the incorporation of two microalgae-based additives (<em>LB-IMMUNOboost</em> and <em>LB-LIVERprotect</em> from LifeBioencapsulation, Almería, Spain) into functional feeds formulated with only 5 % of fishmeal (FM) and low fish oil (FO, 5 %). Four experimental diets were tested for 90 days: i) a formulation mimicking the commercial diets for this species (C+: 20 % FM, 9 % FO); ii) a diet with 5 % of FM based mainly on plant-based ingredients and 5 % FO (C–); and the C– diet supplemented with iii) 1 % <em>LB-IMMUNOboost</em> (IB) or iv) 1 % <em>LB-LIVERprotect</em> (LP). The results showed reduced growth performance, metabolic rates, and overall welfare in fish fed the C– diet. However, IB supplementation in the diet partially reversed the adverse effects produced by the plant-based formulations, improving growth, metabolic parameters, and plasma cortisol levels. These results were consistent with the expression of various genes involved in the stress response, indicating adaptive negative feedback mechanisms within the hypothalamus-pituitary-interrenal (HPI) axis during the secondary stress response after three months of feeding on plant-based diets. The histological findings revealed a significant accumulation of adipocytes around the exocrine intrahepatic pancreas in animals fed plant-based diets (C–, IB, LP). Additionally, intestinal modifications in the subepithelial spaces were observed in these groups, which could be attributed to an increase in intestinal length (ILI, %), leading to the separation of the mucosa and submucosa layers. Nonetheless, fish fed the IB diet and, to a lesser extent, the LP diet, exhibited improvements in epithelial resistance (Rt) and apparent tissue permeability (Papp), which helped counteract the detrimental effects observed in the C– group. Overall, our results suggest that dietary additives like IB can partially mitigate some adverse effects of plant-based raw materials. Yet, given the specific effects of IB and LP on the immune system and hepatic integrity, further studies are needed to fully evaluate their potential benefits.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742873"},"PeriodicalIF":3.9,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fish vitality assessment under adversity stress based on multi-sensing fusion and deep learning techniques","authors":"Yanfei Zhu ,&nbsp;Wenguan Zhang ,&nbsp;Yongjun Zhang ,&nbsp;Xiaoshuan Zhang","doi":"10.1016/j.aquaculture.2025.742871","DOIUrl":"10.1016/j.aquaculture.2025.742871","url":null,"abstract":"<div><div>Accurately assessing the vitality of fish during Cryogenic Waterless Live Transportation (CWLT) is crucial for ensuring their survival rate and transport quality. To address this, this study developed a multi-sensing fusion measurement system, integrating the impedance sensor and image sensor to capture fish impedance, phase angle data and visual image data, which can provide reliable data support for vitality assessment. A multi-input deep learning model for cross-modal data fusion, called the whale optimization algorithm-based dual-channel convolutional network (WOA-DConvNet), was developed to assess fish vitality. The study first conducted a correlation analysis between impedance and phase angle data using blood index data, revealing the relationship between different frequency signals and fish vitality status. Cluster analysis and variance analysis were then performed to examine the significant differences in vitality states. The results show significant differences (<em>P</em> &lt; 0.05) in impedance and phase angle data between different vitality levels. Finally, the WOA-DConvNet model was used to evaluate the vitality of pearl gentian grouper during the live transport. The results showed that the model achieved excellent performance, achieving precision, recall, f1 score and accuracy of 91.81 %, 91.74 %, 91.76 % and 91.71 %. The core goal of this research is to explore the integrated application of artificial intelligence and advanced sensing technologies in the live transport process, aiming to develop a more precise and intelligent vitality assessment method and promote scientific monitoring and management during live fish transportation.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742871"},"PeriodicalIF":3.9,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144469919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early-life Bacillus circulans L38 intervention enhances growth and intestinal health in large yellow croaker (Larimichthys crocea) larvae","authors":"Chuanwei Yao ,&nbsp;Zhihao Zhang ,&nbsp;Yongtao Liu ,&nbsp;Qiuxin Yan ,&nbsp;Jinze Zhang ,&nbsp;Yifan Zhou ,&nbsp;Yayu Qu ,&nbsp;Xianyong Bu ,&nbsp;Kangsen Mai ,&nbsp;Qinghui Ai","doi":"10.1016/j.aquaculture.2025.742819","DOIUrl":"10.1016/j.aquaculture.2025.742819","url":null,"abstract":"<div><div>The early life stage is often regarded as a “window of opportunity” for microbial colonization. Targeted intervening with probiotics during this period may be a beneficial approach to foster the healthy development of larvae. Consequently, the aim of this study was to evaluate the impact of supplemental <em>Bacillus circulans</em> L38 (BC) on survival, growth performance, and intestinal health of large yellow croaker larvae. The larvae were fed diets containing different concentrations of BC (0 CFU/g, 10⁶ CFU/g, 10⁷ CFU/g, and 10⁸ CFU/g) for 30 days. Results demonstrated that larvae fed the diet with 10⁸ CFU/g BC significantly enhanced growth performance (<em>P</em> &lt; 0.05). Concurrently, larvae fed the diet containing 10⁸ CFU/g BC significantly promoted intestinal development by increasing intestinal fold height, enhancing expression of markers related to intestinal epithelial cell proliferation and apical junction complex, and boosting brush border enzyme activity (<em>P</em> &lt; 0.05). Furthermore, larvae fed a diet containing 10⁸ CFU/g BC exhibited a significant enhancement in both the activity and expression levels of digestive and absorption-related enzymes and genes (<em>P</em> &lt; 0.05). Supplementation with 10⁸ CFU/g BC further enhanced the larval antioxidant capacity, likely by modulating both the activity and expression of antioxidant connected to enzymes and genes (<em>P</em> &lt; 0.05). Additionally, supplementation with 10⁷–10⁸ CFU/g BC can alter the activity of enzymes connected to innate immunity and modulate the expression levels of genes involved in inflammatory response, thereby enhancing the immunity of larvae (<em>P</em> &lt; 0.05). Meanwhile, larvae fed a diet containing 10⁸ CFU/g BC maintained healthy intestinal microecology by regulating microbial diversity and boosting the proportion of health-promoting bacteria (<em>P</em> &lt; 0.05). In conclusion, supplementation with 10⁸ CFU/g BC could improve the growth performance and intestinal health of large yellow croaker larvae. This improvement are attributed to the promotion of intestinal development, improvement of digestive and absorptive capacity, augmentation of antioxidant capacity, bolstering of immune function, and optimization of the gut microbiota diversity and composition.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742819"},"PeriodicalIF":3.9,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of algal extracts on the metabolism, intestinal functionality and oxidative status of gilthead of seabream (Sparus aurata)","authors":"F.P. Martínez-Antequera ,&nbsp;P. Simó-Mirabet ,&nbsp;M. Román ,&nbsp;J. Fuentes ,&nbsp;M. Oliva ,&nbsp;D. Méndez-Paz ,&nbsp;R. Vázquez-Sobrado ,&nbsp;J.A. Martos-Sitcha ,&nbsp;F.J. Moyano ,&nbsp;J.M. Mancera","doi":"10.1016/j.aquaculture.2025.742869","DOIUrl":"10.1016/j.aquaculture.2025.742869","url":null,"abstract":"<div><div>Intensive aquaculture systems are associated with decrease in water quality and increased environmental stress and disease prevalence. This promotes the exploration of bioactive compounds that can be included in aquafeeds to enhance fish resilience under such negative conditions. The present study investigated the effects of dietary supplementation with two different extracts obtained from the green seaweed <em>Ulva</em> spp. on the growth performance, intestinal functionality, metabolic profile and oxidative status of gilthead sea bream (<em>Sparus aurata</em>). These extracts, either characterized to be enriched in protein (P) or carbohydrate (F) fractions, were included at 12 % in respective diets. After a 42-day feeding trial, a 10-day starvation challenge was conducted as a putative proof-of-concept on the effects of bioactive compounds under food-deprivation. No significant differences in growth performance were observed between the experimental groups during the feeding period. However, after starvation challenge, fish fed the <em>Ulva</em>-based diets exhibited reduced weight loss (∼5 % lower than the control group), indicating enhanced physiological resilience. No significant effect of the inclusion of algal extracts was observed on intestinal morphology or functionality. Biochemical analyses revealed that plasma lactate levels were significantly lower (∼15 %) in the P group, while plasma protein concentrations were reduced (∼9 %) in the F group compared to the control. Hepatic glycogen depletion was more pronounced in fish fed both supplemented-experimental diets after the starving period. Conversely, oxidative stress markers indicated enhanced antioxidant capacity in both experimental groups. Superoxide dismutase (SOD) activity was significantly higher (∼13 %), while malondialdehyde (MDA) levels, a marker of lipid peroxidation, were ∼ 15 % lower in <em>Ulva</em>-fed fish compared to controls. These findings suggest that <em>Ulva</em> spp. extracts may confer metabolic and antioxidant benefits, particularly under starvation-induced stress, without compromising growth performance. Further research is warranted to optimize inclusion rates and elucidate their long-term impacts.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742869"},"PeriodicalIF":3.9,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring polychaeta meal as a functional ingredient for improving European seabass intestinal health and oxidative status","authors":"M. Monteiro ,&nbsp;R.S. Costa ,&nbsp;J. Silva ,&nbsp;V. Figueiredo ,&nbsp;K. Kousoulaki ,&nbsp;L.M.P. Valente","doi":"10.1016/j.aquaculture.2025.742858","DOIUrl":"10.1016/j.aquaculture.2025.742858","url":null,"abstract":"<div><div>In intensive aquaculture, fish are often exposed to stressors like handling, overcrowding, and confinement, which can compromise health and welfare. Nutrition plays a key role in boosting physiological fitness and stress resilience. Polychaete meal (PM), derived from <em>Alitta virens</em>, is emerging as a promising low-trophic alternative to fishmeal (FM) with potential functional properties. This study evaluated the effects of dietary PM inclusion at 2.5 %, 5 %, and 10 % in European seabass (<em>Dicentrarchus labrax</em>), focusing on intestinal health, non-specific immunity, and oxidative status under both baseline and acute stress conditions. PM diets significantly reduced intestinal immune cell infiltration, particularly at 10 % (PM10), with fewer granulocytes in the anterior intestine (<em>p</em> = 0.009) and reduced lymphocyte presence in the rectum (<em>p</em> = 0.004). Significant metabolic changes were also observed, including significantly increased plasma glucose in fish fed PM10 (<em>P</em> = 0.017) and elevated lactate in the PM2.5 group (<em>P</em> = 0.004), regardless of stress exposure. The PM5 diet significantly enhanced non-specific immune responses, increasing complement activity (<em>p</em> = 0.026) and plasma immunoglobulin levels (<em>p</em> = 0.023). Antioxidant defenses were also significantly improved across all PM groups, with higher GST activity observed in all groups (<em>p</em> = 0.006) and elevated GSH content in PM2.5 and PM5 (<em>p</em> = 0.040). Under acute stress, all groups displayed similar physiological responses, including significant increases in glucose, lactate, and cortisol, as well as altered innate immune markers, without significant differences among diets. These findings suggest that PM can promote health and immune status in European seabass under optimal conditions. Further research is warranted to assess its functional benefits under more challenging aquaculture scenarios.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742858"},"PeriodicalIF":3.9,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144469921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}