Aquaculture最新文献

{"title":"Comparative aquaculture performance of genetically improved triploid oysters of ‘Haida No. 3’ line of the Pacific oyster Crassostrea gigas","authors":"Jianmin Zhou ,&nbsp;Chengxun Xu ,&nbsp;Haijun Liu ,&nbsp;Qi Li","doi":"10.1016/j.aquaculture.2025.742905","DOIUrl":"10.1016/j.aquaculture.2025.742905","url":null,"abstract":"<div><div>In recent decades, the oyster industry has shifted from framing primarily diploid oysters to farming almost exclusively triploids in many regions. This shift has been a major driver of the importance of genetic improvement in triploid performance. Tetraploid oysters play a crucial role in triploid production, contributing two-thirds of the chromosome set to their triploid offspring. Recent selective breeding programs focusing on tetraploid oysters have shown promising results, particularly in improving growth rates. However, it remains unclear how successive generations of tetraploid paternal parents affect the aquaculture performance of their triploid progeny. To address this, four mating combinations of the triploid ‘Haida No. 3’ line were established: HTR3 (diploids ♀ × the third-generation tetraploids ♂), HTR2 (diploids ♀ × the second-generation tetraploids ♂), HTR1 (diploids ♀ × the first-generation tetraploids ♂), HTR0 (diploids ♀ × induced tetraploids ♂). The results showed that triploid oysters derived from higher-generation tetraploid parents exhibited significant improvements in shell height, wet weight, and yield at two farming locations (Rongcheng and Huangdao). On day 480, the selective advantage (SA) for shell height was consistently positive, with values ranging from 9.98 % to 27.49 % across both locations. Additionally, the yield of HTR3 exceeded that of the control group (HTR0) by almost 50 %, indicating a significantly increased production at both locations. Triploidy rates were consistently above 95 % across all groups throughout the life stage, increasing with generations of tetraploid parents. However, the percentage of female β (individuals with inactive gametogenesis) decreased and then increased across successive generations of the tetraploid parents at both sites. Overall, this study demonstrated the potential of selective breeding in tetraploids to sustainably improve the aquaculture production of triploid progeny. It also provided valuable insights for optimizing polyploid breeding programs in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742905"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pond aquaculture dynamics in Asia: Satellite time series for analyzing the spatio-temporal development of coastal aquaculture","authors":"Marco Ottinger ,&nbsp;Kemeng Liu ,&nbsp;Tobias Ullmann ,&nbsp;Juliane Huth ,&nbsp;Claudia Kuenzer ,&nbsp;Felix Bachofer","doi":"10.1016/j.aquaculture.2025.742940","DOIUrl":"10.1016/j.aquaculture.2025.742940","url":null,"abstract":"<div><div>Asia plays a dominant role in global aquaculture, contributing over 88 % of the total aquaculture output, primarily through pond aquaculture systems used for the farming of fish, shrimp, and mussels. Coastal regions in Asia have experienced rapid spatial expansion of pond aquaculture. Recognizing the importance of spatial data in effectively monitoring and managing these systems, we present a methodological approach utilizing dense optical satellite time series archive data to analyze the spatio-temporal development of coastal pond aquaculture in Asia. This study builds on previous work that delineated all aquaculture ponds at a single-pond level for the entire coastal zone using a multi-sensor Earth Observation approach, integrating both SAR and optical satellite data. The resulting continental-scale vector dataset of mapped aquaculture ponds served as reference to derive spatio-temporal dynamics of coastal aquaculture. By utilizing multi-decadal Landsat archive data (1984–2019), we developed a framework to determine the annual status of each pond based on water masks derived from satellite time series. The methodology was applied across 22 coastal countries in South Asia, East Asia and Southeast Asia to investigate the development patterns of pond aquaculture. Using the complete, continental-scale dataset on annual pond aquaculture status, this research conducted geostatistical analyses of growth rates and spatial expansion patterns across various administrative levels, including national and district scales. Growth dynamics were examined for each individual country at 5- and 10-year time intervals. Between the 1980s and 2019, coastal Asia experienced significant expansion of pond aquaculture in time and space, with China, Indonesia, India, Vietnam, and Thailand emerging as the largest contributors. In 1988, reference aquaculture ponds covered around 6500 km², increasing to over 19,000 km² in 2019, representing a more than three-fold increase. Among the examined countries, China maintained the largest pond aquaculture industry, accounting for 40.6 % of the total active pond area in 2019, followed by Indonesia (13 %) and India (11.2 %).</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742940"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144749051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive evaluation and analysis of cold tolerance in Neopyropia yezoensis strains based on multiple traits","authors":"Zi-Xiang Zhai ,&nbsp;Guo-Ying Du ,&nbsp;Lan Wu,&nbsp;Lin Tian,&nbsp;Li-Hong Ma","doi":"10.1016/j.aquaculture.2025.742875","DOIUrl":"10.1016/j.aquaculture.2025.742875","url":null,"abstract":"<div><div><em>Neopyropia yezoensis</em>, an important economic macroalga, is cultivated in the coastal regions of Northeast Asia, where the farming season spans the cold winter. The low temperatures during this period severely impacting its growth and yield. Given these challenges, selecting high-quality strains with enhanced cold tolerance is essential for improving both yield and quality. To achieve this, it is crucial to establish a set of evaluation criteria and methods to effectively screening for cold-tolerant germplasm. In this study, the cold tolerance of 60 <em>N. yezoensis</em> strains was evaluated by assessing 13 traits, including growth rate, photosynthetic parameters, and pigment contents. Under low temperature, most traits were depressed, with the daily growth rate (DGR) being particularly affected. Principal Component Analysis (PCA) and weight analysis identified thalli area, DGR, chlorophyll <em>a</em> (Chl <em>a</em>), phycoerythrin (PE), allophycocyanin (APC) and photochemical efficiency (Φ_PSII) as primary contributors to cold tolerance. A comprehensive index (D) was developed based on the membership function and weight of each trait to rank the 60 strains into five cold tolerance levels. Over 65.0 % of strains exhibited a certain extent of cold tolerance, which aligning with the nature <em>N. yezoensis</em> as cold-water species. The optimal evaluation model was established as D = 0.387a rea + 0.122Chl <em>a</em> + 0.242F_v/fm + 0.066APC - 0.086, with R² of 0.955 (where F_v/fm represents the maximum photochemical efficiency). This research establishes a comprehensive evaluation system for cold tolerance in <em>N. yezoensis</em>, enabling the rapid screening of cold-tolerant strains and enhancing breeding efforts in the industry.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742875"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary niacin supplementation enhances growth and osmoregulation of Nile tilapia (Oreochromis niloticus) under hyperosmotic stress improving ion homeostasis and carbohydrate metabolism","authors":"Yuxi Yan ,&nbsp;Jinquan Fan ,&nbsp;Wei Liu,&nbsp;Minxu Wang,&nbsp;Erchao Li,&nbsp;Liqiao Chen,&nbsp;Xiaodan Wang","doi":"10.1016/j.aquaculture.2025.742881","DOIUrl":"10.1016/j.aquaculture.2025.742881","url":null,"abstract":"<div><div>The aim of this experiment was to investigate the effects of dietary niacin supplementation on the growth, osmoregulatory capacity, and energy metabolism of Nile tilapia (<em>Oreochromis niloticus</em>) under salinity stress. In this study, five diets with varying niacin concentrations were prepared, with practical niacin levels of 3.33, 12.18, 33.98, 94.28, and 275.23 mg/kg. Fish with an initial weight of 1.80 ± 0.22 g were selected for an 8-week feeding trial under 20 psu salinity. The results indicated that the dietary niacin significantly increased the weight gain, specific growth rate, and condition factor of tilapia. Furthermore, niacin supplementation also increased the crude protein and crude fat contents of tilapia. 12.18–94.28 mg/kg dietary niacin significantly improved the ion transport capacity of the gills which is a main tissue of the osmoregulation. Histological analysis of gill revealed that dietary niacin at concentrations ranging from 12.18 to 275.33 mg/kg relieved structural damage from salinity stress. After ingestion, niacin primarily accumulated in the liver and gills, activating the niacin metabolic pathway. As a result, compare to the control group, fish fed with dietary niacin got higher level of NAD⁺, NAD⁺/NADH. The addition of 94.28 mg/kg niacin affected the glycolysis, TCA cycle and gluconeogenesis processes through SIRT1/GLUT1/HK signaling pathway. In addition, content of liver glucose was decreased, while pyruvate and ATP contents were increased. Optimal dietary niacin could improve the growth performance of Nile tilapia under salinity stress by activating the carbohydrate metabolism by SIRT1/GLUT1/HK signaling pathway. It can also enhance the salinity tolerance by activating ion transportation. Based on the second−order polynomial regression analysis with weight gain rate and specific growth rate, the optimal dietary niacin for Nile tilapia under salinity stress is 151.67–171.73 mg/kg.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742881"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant gonadotropin induced out-of-season spawning and larval culture of flathead grey mullet (Mugil cephalus)","authors":"Giancarlo Cerrud-Barría ,&nbsp;Alicia Estévez ,&nbsp;Sandra Ramos-Júdez ,&nbsp;Ignacio Giménez ,&nbsp;Neil Duncan","doi":"10.1016/j.aquaculture.2025.742908","DOIUrl":"10.1016/j.aquaculture.2025.742908","url":null,"abstract":"<div><div>The flathead grey mullet (<em>Mugil cephalus</em>) held in intensive captive condition does not sexually mature. The present study, aimed to apply recombinant gonadotropin (rGth) protocols to advance spawning by three months and demonstrate the protocols potential for juvenile production. Eight females and seven males were divided into an rGth group that was treated with species specific recombinant follicle stimulating hormone and recombinant luteinizing hormone and a control group treated with saline. The rGths-treated males completed spermiation and the females completed vitellogenesis and spawning in June–July, three months before the natural spawning period (September – October). Control females did not advance with oogenesis and control males did not advance to spermiation. Eggs were incubated, and hatched larvae were reared in three replicated densities (16, 32, and 51 larvae/L, 100 L tanks) and in grey and black 500 L tanks at 30 larvae/L. Final survival at 16 larvae/L was 14.13 ± 5.2 % at 42 days post- hatching (dph) and a correlation between survival and stocking density predicted 3 juveniles/L could be obtained at 42 dph with an initial density of 38 larvae/L. Larvae reared in black tanks had a superior growth rate (16.70 ± 0.05 mm) in comparison to the grey tanks (11.40 ± 2.80 mm) at 42 dph (<em>P</em> <em>= 0.01</em>). Together, these findings confirm the high efficacy (close to 100 %) of rGths induction protocols, that out-of-season spawning can be induced, and that rGth induced mullet spawning has good potential as a bases for juvenile production.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742908"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144548367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermented fish meal derived from barramundi by-product enhances growth, immunity, and gut microbiota in white shrimp Penaeus vannamei","authors":"Ann-Chang Cheng ,&nbsp;Rui-Jun Kang ,&nbsp;Chun-Hung Liu","doi":"10.1016/j.aquaculture.2025.742876","DOIUrl":"10.1016/j.aquaculture.2025.742876","url":null,"abstract":"<div><div>This study investigated the effects of barramundi, <em>Lates calcarifer</em>, by-product fish meals on the growth, immunity, and gut microbiota of white shrimp, <em>Penaeus vannamei</em>. Three variants were assessed: untreated fish meal (BFM), enzymatically hydrolyzed fish meal (EBFM), and fermented fish meal (FBFM), alongside a commercial fish meal control. Compared to commercial fish meal, barramundi by-product meals exhibited lower crude protein but higher lipid and ash contents. Fermentation with <em>Lactobacillus plantarum</em> 7–40 notably increased methionine levels in FBFM. Shrimp fed diets containing EBFM or FBFM showed significantly greater weight gain, feed efficiency, and biomass production, with FBFM yielding the most pronounced improvements. FBFM supplementation also enhanced immune parameters, including phenoloxidase, respiratory burst, superoxide dismutase, lysozyme, and phagocytic activities, alongside upregulation of immune-related genes. Post-challenge survival against <em>Vibrio alginolyticus</em> was highest in the FBFM group. Gut microbiota analysis revealed reduced <em>Vibrio</em> abundance and increased beneficial genera (e.g., <em>Enterococcus</em>, <em>Rubritalea</em>) in FBFM-fed shrimp. Principal component analysis indicated distinct microbial profiles among treatments. These results highlight FBFM as a viable alternative protein source that improves shrimp growth, immunity, gut health, and disease resistance, supporting sustainable aquafeed development within a circular economy framework.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742876"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144470577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastic pollution in aquaculture species: Quantification and characterization in Tilapia and Pangas","authors":"Md. Rased Khan Manon ,&nbsp;Md. Sazedul Hoque ,&nbsp;Md. Ariful Alam ,&nbsp;Md. Saiful Islam ,&nbsp;Md. Arifur Rahman","doi":"10.1016/j.aquaculture.2025.742922","DOIUrl":"10.1016/j.aquaculture.2025.742922","url":null,"abstract":"<div><div>Microplastics (MPs) are an emerging threat to freshwater ecosystems and aquatic life, yet data on MPs pollution in aquaculture systems especially in Bangladesh, remain scarce. This study investigated the tissue accumulation and polymer compositions of MPs influenced by the feeding habits of <em>Oreochromis niloticus</em> and <em>Pangasius hypophthalmus</em> from aquaculture ponds. MPs were predominantly detected in the gastrointestinal tract (GIT) and muscle tissues, with fibers (&lt;0.5 mm) being the most common type and black the dominant color. Interestingly, feeding habits did not significantly affect the overall MP intake. Principal component analysis (PCA) revealed 70.3 % of the total variation in Tilapia and 53.7 % in Pangas, with 1 to 5, transparent, and filament (fiber) showing significant positive contributions as the main components. Fourier-transform infrared spectroscopy (FTIR) analysis detected polyethylene as the most abundant polymer, followed by polyester, polypropylene, cellulose, non-plastic, and unidentified particles. These findings highlight the prevalent presence of MPs in aquaculture systems, raising concerns about trophic transfer and eventual risks to human health. The study highlights the pressing need for operational management policies to alleviate MP pollution in aquaculture environments.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742922"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential role of AMP-activated protein kinase (AMPK) in regulating lipid metabolism in the Manila clam Ruditapes philippinarum under hyposalinity stress","authors":"Tao Liu ,&nbsp;Hongtao Nie ,&nbsp;Jianfeng Ding ,&nbsp;Zhongming Huo ,&nbsp;Xiwu Yan","doi":"10.1016/j.aquaculture.2025.742901","DOIUrl":"10.1016/j.aquaculture.2025.742901","url":null,"abstract":"<div><div>Lipid metabolism is considered to play a crucial role in assisting aquatic animals to adapt to environmental change. In the Manila clam, <em>Ruditapes philippinarum</em>, the involvement of AMPK in the regulation of lipid metabolism under hyposalinity stress is not well understood. Hence, in this study, two populations (TS wild population and FZ culture population) of <em>R. philippinarum</em> were employed in experimentation on the effects of hyposalinity stress. The enzyme activities of triglyceride, total cholesterol, and free fatty acid in the clam hepatopancreas significantly decreased under hyposalinity. Conversely, a significant increase in <em>AMPK</em> gene expression was observed. Under hyposalinity stress, the levels of <em>SREBP-1c</em>, <em>ACC</em>, and <em>SCD</em> gene expression were decreased, whereas the levels of <em>CPT1</em> and <em>ACOX1</em> expression were increased. The results indicate that the <em>AMPK</em> gene may be activated under hyposalinity. This activation could subsequently inhibit lipid metabolism, promote lipid oxidation, provide energy for osmotic pressure regulation, and maintain energy balance. Our study delivers important insights into the molecular mechanisms by which AMPK regulates lipid metabolism, energy metabolism, and osmotic regulation in <em>R. philippinarum</em> under hyposalinity stress. Additionally, it offers a valuable reference for the breeding and farming of <em>R. philippinarum</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742901"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CLIP-AFIR: A Contrastive Language-Image Pretraining Model for Accurate Fish Individual Fine-grained Re-identification","authors":"Jianing Quan ,&nbsp;Can Wang ,&nbsp;Yunchen Tian","doi":"10.1016/j.aquaculture.2025.742885","DOIUrl":"10.1016/j.aquaculture.2025.742885","url":null,"abstract":"<div><div>Similar to identifying different human individuals, accurately determining ”who is who” is a critical component in intelligent aquaculture, with broad applications in disease analysis, growth monitoring, and other aspects. The accuracy of fish Re-Identification (ReID) has greatly improved currently, yet challenges remain in distinguishing the same species, such as low precision and ID switching. Inspired by the multimodal large models, we propose a Contrastive Language-Image Pretraining model for Accurate Fish Individual fine-grained Re-identification (CLIP-AFIR). Based on the cross-modal contrastive learning, a set of trainable text tokens is introduced to represent different individuals, combined with the proposed Prompt Learner Module (PLM). The text and image encoders are trained through the two-stage paradigm, which enhances the adaptability in fish recognition. To improve the ability to discriminate subtle individual differences, a lightweight Fine-grained Feature Enhancement Module (FFEM) is further designed. By using shifted windows with overlapping regions to compute mask self-attention on local areas of the image, it enables effective representation of fine-grained local variations. On the constructed grouper dataset, the proposed CLIP-AFIR shows a significant improvement in the evaluations. Applied to the non-continuous fish individual recognition task, CLIP-AFIR achieves an accuracy of 98.6%, surpassing the state-of-the-art by 5.4 points.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742885"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144572278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptation and domestication of endangered wild betta fish (Betta channoides) originating from East Kalimantan, Indonesia","authors":"Agus Priyadi ,&nbsp;Asep Permana ,&nbsp;Bastiar Nur ,&nbsp;Sawung Cindelaras ,&nbsp;Siti Murniasih ,&nbsp;Rendy Ginanjar ,&nbsp;Sulasy Rohmy ,&nbsp;Lukman ,&nbsp;Djamhuriyah S. Said ,&nbsp;Muhamad Yamin ,&nbsp;Lili Sholichah ,&nbsp;Erma Primanita Hayuningtyas ,&nbsp;Murdinah ,&nbsp;Dorota Katarzyna Fopp-Bayat ,&nbsp;Suciyono ,&nbsp;Darmawan Setia Budi","doi":"10.1016/j.aquaculture.2025.742900","DOIUrl":"10.1016/j.aquaculture.2025.742900","url":null,"abstract":"<div><div><em>Betta channoides</em> is an endangered freshwater fish endemic to Indonesia. This study was conducted to establish a methodology for adapting and domesticating <em>B. channoides</em> through breeding and restocking initiatives. Fish were successfully acclimated and bred in captivity in Depok City, West Java, Indonesia, which is significantly distant from its native habitat in East Kalimantan. A total of 100 immature fish (50 males and 50 females) were collected from the wild. The study consisted of three main experiments: (1) evaluation of rearing containers (Styrofoam box, plastic container, and glass aquarium) for 30 days with three replicates using five males and females (maintained separately); water temperature, pH, and ammonia were 27.5–30.8 °C, 5.2–5.6, and 0.004 mg/L, respectively; (2) gonadal maturation using <em>Moina</em> sp., mosquito larvae (<em>Culex</em> sp.), and bloodworms (<em>Chironomus</em> sp.) for 30 days without replication, using eight males and females in glass aquariums with water quality of 27.9–30.8 °C, 5.3–5.7, and 0.004 mg/L; and (3) breeding of 12 male–female pairs. A glass aquarium was the most suitable rearing container for inducing minimal stress, as indicated by the lowest blood glucose levels recorded. <em>Chironomus</em> sp. larvae served as the optimal nutrition source for maturation, resulting in enhanced weight gain and the highest number of mature fish. The number of larvae produced was 30.67 ± 9.23 individuals per spawning. After the yolk absorption period of 7 days, the larvae were fed for the first time. This study provides essential data on the reproduction and early development of <em>B. channoides</em> to support conservation aquaculture strategies.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742900"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}