Aquaculture最新文献

{"title":"Dietary Egyptian mallow attenuates metiram-evoked neuro-ethological, endoplasmic reticulum stress genes, and biochemical/histopathological disruptions in Oreochromis niloticus","authors":"Afaf N. Abdel Rahman ,&nbsp;Heba Said Abdel-Rahman Gharib ,&nbsp;Amany I. Ahmed ,&nbsp;Asmaa W. Zaglool ,&nbsp;Tarek Khamis ,&nbsp;Mohammed S. Sobh ,&nbsp;Refaat Ras ,&nbsp;Yasser Abd Elhakim ,&nbsp;Rowida E. Ibrahim","doi":"10.1016/j.aquaculture.2025.742193","DOIUrl":"10.1016/j.aquaculture.2025.742193","url":null,"abstract":"<div><div>The improper application of pesticides including fungicides generates serious biological risks that may compromise aquatic biota and inhibit sustainable aquaculture. Consequently, this pioneering attempt looked at the ability of the leaves of <em>Malva parviflora</em> (Egyptian mallow) powder (MPP) to counteract various toxicological consequences in <em>Oreochromis niloticus</em> (Nile tilapia) caused by metiram (MEM) exposure. The investigation encompassed neuro-ethology, stress biomarkers, gene expression associated with endoplasmic reticulum (ER) stress, and histopathology assays of gill and brain tissues. Accordingly, fish (32.80 ± 0.92 g body weight; <em>n</em> = 200) were haphazardly assigned into four groups in five replicates for nine weeks (control, MPP, MEM, and MPP + MEM). The nominal concentration of MEM was 0.377 mg/L (1/10 of 96 h-lethal concentration 50). Dietary MPP level was included at 15 g/kg diet concentration. The outcomes revealed lower fish survivability (68.00 %) and significant (<em>P</em> &lt; 0.05) various behavioral changes (declining in foraging, swimming, aggressive and line crossing, and increasing in surfacing, resting, and abnormal movement) by chronic MEM exposure. The neuro-stress biomarkers were significantly (<em>P</em> &lt; 0.001) altered by MEM exposure, where 8-hydroxy-2′-deoxyguanosine, glucose, and cortisol increased with no marked change in acetylcholine esterase activity. In addition, the MEM exposure induced a prolonged ER stress which was proven by significant (<em>P</em> &lt; 0.001) up-regulation of Jun-N-terminal kinase, C/EBP homologous protein, and extracellular signal-regulated kinase-1 genes of gills and mechanistic target of rapamycin kinase, protein kinase RNA-like ER kinase, activating transcription factor 6, and binding immunoglobulin protein genes of brain. Histologically, it considerably generated a wide variety of changes in gills (detachment, inflammatory cell infiltration, and congestion) and brain (vacuolation, degeneration, and marked aggregation of glial cells). Interestingly, the dietary intervention of MPP significantly (<em>P</em> &lt; 0.05) streamlined these variables by nearly renovating to control levels while preserving the histological integrity of the gills and brain tissues. The outputs of this perspective suggest the dietary intervention of MPP to safeguard against MEM toxicity in <em>O. niloticus</em> for sustaining aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742193"},"PeriodicalIF":3.9,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/CasRx: A novel antiviral approach to combat largemouth bass (Micropterus salmoides) Rhabdovirus infections","authors":"Kechen Yang ,&nbsp;Baoxia Ma ,&nbsp;Zhenmin Wu ,&nbsp;Yao Wang ,&nbsp;Sen Yang ,&nbsp;Fei Ling ,&nbsp;Tianqiang Liu ,&nbsp;Kun Xu ,&nbsp;Gaoxue Wang","doi":"10.1016/j.aquaculture.2025.742189","DOIUrl":"10.1016/j.aquaculture.2025.742189","url":null,"abstract":"<div><div>The Largemouth bass (<em>Micropterus salmoides</em>), a freshwater species of considerable economic significance native to North America, is currently facing a formidable challenge from the <em>Micropterus salmoides</em> rhabdovirus (MSRV). This viral pathogen has emerged as a primary agent of mortality within the species, with its recurrent outbreaks posing a substantial impediment to the sustainable and ecologically sound cultivation of Largemouth bass. The absence of a viable control strategy has underscored the urgent need for innovative approaches to mitigate the impact of MSRV on the aquaculture industry, thereby preserving the integrity and health of this valuable fishery resource. This study presents the development and validation of a CRISPR/CasRx-based approach to target and reduce MSRV replication. This study designed specific CRISPR/CasRx RNAs (crRNAs) to target the MSRV's five structural proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and large protein (L). The construction of a CasRx-MSRV plasmid system was achieved and demonstrated potent antiviral activity against MSRV in both <em>in vitro</em> and <em>in vivo</em> models. Our initial evaluation focused on the CRISPR/CasRx system's ability to inhibit MSRV replication in GCO cells, with a particular emphasis on the L protein. The CasRx-MSRV-L system achieved a significant 97.79 % inhibition rate, corresponding to a five-log reduction in viral titer. This finding prompted us to further explore the therapeutic and prophylactic potential of the CasRx-MSRV-L system against MSRV infection in largemouth bass. While therapeutic application modestly extended the survival of infected fish, prophylactic administration significantly improved the survival rate of juvenile fish by 79.22 %. Collectively, these results underscore the promise of the CRISPR/CasRx system as a novel and potent tool for conferring resistance against MSRV, with broader implications for combating RNA viruses. Furthermore, our findings contribute to the burgeoning body of evidence supporting the application of CRISPR/CasRx technology as a strategic approach to combat RNA viruses, thereby laying a robust theoretical foundation for future research and development in this field.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742189"},"PeriodicalIF":3.9,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the attribution of bivalve-macroalgae polyculture as a carbon source or sink from an ecosystem perspective: A case study of kelp-oyster IMTA","authors":"Yumeng Xie ,&nbsp;Xiaofang Qin ,&nbsp;Jinghui Fang ,&nbsp;Jialei Xu ,&nbsp;Yitao Zhang ,&nbsp;Junwei Wang ,&nbsp;Xiaowen Zhang","doi":"10.1016/j.aquaculture.2025.742165","DOIUrl":"10.1016/j.aquaculture.2025.742165","url":null,"abstract":"<div><div>To clarify interactions among bivalves, macroalgae, and water column in the bivalve-macroalgae polyculture system, in-situ mesocosm cultivation was conducted for 24 h in both summer and winter. These experiments assessed seasonal variations in CO₂ and components of dissolved inorganic carbon (DIC), nitrogen (DIN), and phosphorus (DIP) under three modes: monoculture of three sizes of oysters (<em>Crassostrea gigas</em>) (OA group), monoculture of kelp (<em>Saccharina latissima</em>) (KA group), and polyculture of oysters and kelp (KO group) at fixed wet weight ratio of 800: 200 g. The results revealed the OA group functioned as a CO₂ source in both summer and winter, with summer increase rates of DIC, DIN, and DIP by oysters much higher than in winter and decreasing with larger oyster size. Pearson correlation analysis indicated a negative correlation between soft tissue dry weight of oysters and concentrations of NO₂⁻-N and NH₄⁺-N. While, the KA group functioned as a CO₂ sink, with winter decrease rates of DIC, DIN, and DIP by kelp being 14.24, 3.16, and 5.54 times greater than in summer, respectively. In the KO groups, oyster size and seasonal factors significantly affected water parameters, including pH, dissolved oxygen (DO), <em>p</em>CO₂, and various DIC, DIN, and DIP components (<em>P</em> &lt; 0.05) and the system released DIC, DIN, and DIP in summer while absorbing them in winter. Subtraction calculations indicated that the culturing water in KO groups absorbed CO₂, DIC, DIN, and DIP during both seasons, suggesting a synergistic bioremediation effect between phytoplankton and macroalgae at an appropriate cultivation ratio.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742165"},"PeriodicalIF":3.9,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood-brain barrier permeability and brain immunity in yellow catfish (Pelteobagrus fulvidraco) responding to Edwardsiella ictaluri infection","authors":"Qin Wang ,&nbsp;Li Shen ,&nbsp;Mengge Guo ,&nbsp;Bingchao Wang ,&nbsp;Huimin Liu ,&nbsp;Ke Xiao ,&nbsp;Zechao Shi ,&nbsp;Wei Ji","doi":"10.1016/j.aquaculture.2025.742179","DOIUrl":"10.1016/j.aquaculture.2025.742179","url":null,"abstract":"<div><div>The blood-brain barrier (BBB) plays a crucial role in maintaining the homeostasis of the central nervous system (CNS) by preventing the entry of harmful substances into the brain parenchyma. Although bacterial infections of the CNS are typically linked to BBB disruption, there is a notable paucity of knowledge concerning alterations in BBB permeability and the CNS immune response in teleosts during such infections. This study investigates these phenomena by administering an intraperitoneal injection of <em>Edwardsiella ictaluri</em> to yellow catfish (<em>Pelteobagrus fulvidraco</em>), followed by an evaluation of BBB permeability and the CNS immune response. <em>E. ictaluri</em> has the capability to penetrate the BBB and predominantly colonizes the mesencephalon region. Furthermore, there was a significant elevation in reactive oxygen species (ROS) activity within both the telencephalon and mesencephalon, accompanied by a reduction in mRNA expression levels of tight junction (TJ) proteins, which coincided with increased BBB permeability. Additionally, pathological alterations in brain tissue structure were observed following <em>E. ictaluri</em> infection.The immune response of the CNS was triggered by <em>E. ictaluri</em> infection, which primarily affected the functionality of the neuropeptide signaling pathway and chemokine activity. Notably, <em>E. ictaluri</em> infection resulted in a significant increase in the amount of B cells in the mesencephalon and elevated antibody levels. According to these findings, <em>E. ictaluri</em> enhanced the permeability of the BBB by rupturing the TJs between endothelial cells. This disruption induced inflammation and resulted in an increased presence of immunoglobulins and B cells within the CNS.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742179"},"PeriodicalIF":3.9,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbohydrates in dietary ingredients for European seabass: Impact on nutrient digestibility and waste production when reared in recirculating aquaculture systems","authors":"Elisavet Syropoulou ,&nbsp;Satya Prakash ,&nbsp;Daan Smeenge ,&nbsp;Detmer Sipkema ,&nbsp;Johan W. Schrama ,&nbsp;Fotini Kokou","doi":"10.1016/j.aquaculture.2025.742182","DOIUrl":"10.1016/j.aquaculture.2025.742182","url":null,"abstract":"<div><div>The growing demand for aquafeeds along with the limited availability of fish meal has necessitated the use of various alternative ingredients in feed formulations. Depending on the ingredient, such use may also entail higher and diverse carbohydrate inclusion in the diets with implications for fish digestion and environmental output in terms of waste production. The present study examined the impact of different dietary ingredients on nutrient digestibility and waste production focusing on faecal removal efficiency when used in recirculating aquaculture systems (RAS). For this purpose, a trial was performed with juvenile European seabass (<em>Dicentrarchus labrax</em>), testing seven ingredients with different carbohydrate levels and types (starch and non-starch polysaccharides, NSP): wheat dried distillers grain with solubles, DDGS; hydrolyzed feather meal I, HFM I; hydrolyzed feather meal II, HFM II; insect meal (black soldier fly larvae, <em>Hermetia illucens</em>), IM; single-cell protein, SCP; shrimp-shell meal, SSM; seaweed protein, SWP. The test ingredients were included in a basal (control, CTR) diet at a level of 15 %, and fish were fed restrictively for a period of 4 weeks. Apparent nutrient digestibility was measured, with a focus on carbohydrates, and faecal quantity was determined based on organic matter (OM) digestibility values. Waste production was evaluated based on the amount of removed faeces by settling, and faecal particle size distribution. The results showed that starch digestibility decreased with increasing dietary starch levels, whereas NSP digestibility varied depending on the ingredient source. Moreover, faecal waste production had a stronger correlation with dietary NSP compared to starch. High inclusion levels of both nutrients in the diet were correlated with reduced faecal removal efficiency by settling. Overall, the DDGS and SWP diets scored worse for all indicators used here to assess waste production, whilst SSM excelled at producing highly settleable faeces compared to the rest of the diets. Summarizing, the current findings suggest that the type of dietary ingredients, reflecting also the type and level of dietary carbohydrates, determines faecal quantity and quality, and therefore careful selection of ingredients for RAS aquafeeds should be considered in this context.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742182"},"PeriodicalIF":3.9,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo antiviral effect of molnupiravir against infectious hematopoietic necrosis virus","authors":"Linfang Li ,&nbsp;Chunyan Guan ,&nbsp;Tongyan Lu ,&nbsp;Jingzhuang Zhao ,&nbsp;Yizhi Shao ,&nbsp;Liming Xu","doi":"10.1016/j.aquaculture.2025.742187","DOIUrl":"10.1016/j.aquaculture.2025.742187","url":null,"abstract":"<div><div>Infectious hematopoietic necrosis virus (IHNV) is the pathogen of infectious hematopoietic necrosis (IHN), which is a significant threat to the salmon and trout industry. To develop drugs that can inhibit IHNV, we screened a library containing 609 compounds. Molnupiravir, a nucleoside analogue, was found to effectively inhibit the <em>in vitro</em> infection of IHNV with EC₅₀ of 5.554 μM in this study. The targeting period of anti-IHNV effect was determined by the time of addition essay and the expression change of virus gene in attachment, internalization, genome synthesis, and release stages with drug treatment. The results showed that the molnupiravir targeted the early stages of the IHNV life cycle by affecting the attachment stage, and it also significantly decreased the specific infectivity (<em>si</em>) value of IHNV, a parameter evaluating the phenomenon of restricted virus/cell interaction. Molnupiravir was administrated through oral and intraperitoneal injections, respectively. The mortality rate caused by IHNV infection was significantly reduced in the fish that were intraperitoneally injected molnupiravir with a single dosage of 10 mg/kg (<em>P</em>&lt;0.05). These findings suggest that molnupiravir has the possibility to be used as an antiviral drug for the prevention and control of IHN.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742187"},"PeriodicalIF":3.9,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics integrative analysis reveals the molecular mechanisms of muscle adaptive changes in largemouth bass (Micropterus salmoides) under water flow stress in recirculating aquaculture","authors":"Lei Wang ,&nbsp;Chang Liu ,&nbsp;Lingran Wang ,&nbsp;Jiaxin Xu ,&nbsp;Jiajun Zheng ,&nbsp;Yuyue Xu ,&nbsp;Zhan Jin ,&nbsp;Di Feng ,&nbsp;Meng Zhang ,&nbsp;Miao Yu ,&nbsp;Hongxia Jiang ,&nbsp;Zhigang Qiao","doi":"10.1016/j.aquaculture.2025.742172","DOIUrl":"10.1016/j.aquaculture.2025.742172","url":null,"abstract":"<div><div>To elucidate the molecular mechanisms underlying changes in muscle quality of largemouth bass (<em>Micropterus salmoides</em>) under water flow stress, a 90-day experiment was conducted. A total of 270 fish were randomly assigned to three groups, each comprising three replicates with 30 fish per tank: sustained exercise (SE), interval exercise (IE), and control (CG). Dorsal white muscle samples were collected for transcriptomic and metabolomic analyses. Transcriptomic sequencing identified 395 differentially expressed genes (DEGs) between IE and CG (207 upregulated, 188 downregulated) and 353 DEGs between SE and CG (152 upregulated, 201 downregulated). Metabolomic analysis revealed 113 metabolites upregulated and 68 downregulated in IE, whereas 219 metabolites were upregulated and 172 downregulated in SE compared to CG. Integrated transcriptomic and metabolomic analyses suggest that water flow stress enhances muscle quality in largemouth bass through several key pathways, including the Apelin signaling pathway, the calcium signaling pathway, and the taurine and hypotaurine metabolism pathways. Notably, the upregulation of sphingosine 1-phosphate in the Apelin and calcium signaling pathways contributes to muscle function stability and overall health. Similarly, the upregulation of pyruvate and taurine in the taurine and hypotaurine metabolism pathway indicates enhanced energy metabolism and antioxidant responses during sustained exercise, enabling muscles to meet energy demands and mitigate oxidative stress caused by intense exercise. These findings provide valuable insights into the molecular mechanisms by which water flow stress improves muscle quality in largemouth bass. This research serves as a scientific reference for optimizing muscle quality in largemouth bass within recirculating aquaculture systems (RAS).</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742172"},"PeriodicalIF":3.9,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143292596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modelling the impact of rainfall events on increasing concentrations of Vibrio vulnificus in New Zealand farmed Pacific oysters (Crassostrea gigas)","authors":"Graham C. Fletcher ,&nbsp;Roland Taylor ,&nbsp;Duncan Hedderley","doi":"10.1016/j.aquaculture.2024.742094","DOIUrl":"10.1016/j.aquaculture.2024.742094","url":null,"abstract":"<div><div><em>Vibrio vulnificus</em> occurs naturally in seawater and causes debilitating, often fatal illnesses, particularly in people with underlying health issues such as liver disease. The illness can occur when raw molluscan shellfish that have bio-accumulated the organism are consumed. New Zealand seafood is not known to have caused any illnesses, although there have been wound infections from the organism. This study sought to better understand the effect of environmental conditions on concentrations of <em>V. vulnificus</em> in farmed Pacific oysters (<em>Crassostrea gigas</em>) in three harbours over the summer-autumn months (December to May) of 2016–19. Fortnightly sampling at two harbours only once gave a most probable number (MPN) of &gt;30 per g of oyster meat (regulatory guideline for processed shellfish), while the third harbour regularly produced much higher counts (up to 220,000 per g). From 2017 to 19, weekly samples from three sites in this harbour (four in 2018) were tested. Eleven peaks in concentration were observed, all when seawater temperatures exceeded 20 °C and after heavy rainfall had reduced the seawater salinity, usually to &lt;25 ‰ from the average of 32 ‰. A fitted structural equation model with temperature and salinity terms accounted for 60 % of the variance in concentrations and rates of decline after concentration peaks averaged 1.05 log₁₀ MPN per week. The rate of decline was highly variable so microbiological testing would be required to confirm this rate for use in food safety management. However, the results of the study will enable better risk management.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742094"},"PeriodicalIF":3.9,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary carvacrol essential oil alleviates thallium nitrate-induced toxicity in Oreochromis niloticus: Growth, hepatorenal, oxidative stress, gene expression, and histopathological study","authors":"Dalia A. Abdel-moneam ,&nbsp;Hanan S. Khalefa ,&nbsp;Maha M. Rashad ,&nbsp;Ghada E. Ali ,&nbsp;Dina W. Bashir ,&nbsp;Peter A. Noshy ,&nbsp;Sahr B. Mahmoud","doi":"10.1016/j.aquaculture.2025.742175","DOIUrl":"10.1016/j.aquaculture.2025.742175","url":null,"abstract":"<div><div>Natural dietary supplements are sought to mitigate fish intoxication by water contaminants. Thallium (TI) exposure poses a potential hazard to aquatic systems. Therefore, the present study investigated the possible supportive role of carvacrol essential oil (CAR) against thallium nitrate (TINO₃) toxicity in <em>Oreochromis niloticus</em> (Nile tilapia) concerning different fish stress biomarkers and histopathological alterations. One hundred and eighty fish (35.00 ± 1.00 g) were randomly distributed into four groups, each with three replicates. The first group served as the negative control, the second group was exposed to 1/10 of the 96-h median lethal concentration (LC₅₀) of TINO₃ (41.9 μg/L), the third group received a supplemented diet containing CAR (0.5 g/kg diet), and the fourth group received both TINO₃ and CAR concurrently. At the end of the 30-day experimental duration, results showed that fish in the TINO₃ group exhibited abnormal swimming patterns, skin melanosis, and decreased growth performance and survivability. Additionally, there were marked increases in serum alanine aminotransferase and aspartate aminotransferase enzymes and elevated urea and creatinine levels. A significant increase in malondialdehyde and a significant decrease in reduced glutathione levels were observed. Furthermore, there was significant down-regulation of the glutathione peroxidase and glutathione S-transferase antioxidant genes, along with up-regulation of stress-related genes, including cytochrome P450 and heat shock protein 70, compared with the negative control group (<em>p</em> &lt; 0.05). Histopathological examination revealed several alterations, including disorganization and congestion of gill lamellae, hepatosplenic congestion, vacuolar degeneration, and irregular skeletal muscle fibers in the TINO₃-exposed group. Dietary CAR co-treatment mitigated TI-induced stress and nearly restored the aforementioned variables to control values while improving the histological architecture of affected tissues. Therefore, CAR may be utilized as an effective dietary supplement in aquaculture to counteract water toxicants.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742175"},"PeriodicalIF":3.9,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143293368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functions of the transcription factor myb-like in sex determination and differentiation in Portunus trituberculatus","authors":"Shaoting Jia ,&nbsp;Weikang Lan ,&nbsp;Yuchao Huang ,&nbsp;Yichen Su ,&nbsp;Baoquan Gao ,&nbsp;Ping Liu ,&nbsp;Jianjian Lv","doi":"10.1016/j.aquaculture.2025.742173","DOIUrl":"10.1016/j.aquaculture.2025.742173","url":null,"abstract":"<div><div>The growth traits of <em>Portunus trituberculatus</em> show sexual dimorphism; therefore, it is important to explore and identify the functional genes related to sex determination and differentiation to establish monosexual breeding technology. Herein, the DNA sequence of <em>myb-like</em>, located in the sex-determining region, was obtained using comparative genomics analysis. The results of quantitative real-time reverse transcription PCR and <em>in situ</em> hybridization indicated that <em>myb-like</em> is specifically expressed in male individuals. Functional study of <em>myb-like</em> by eyestalk ablation and RNA interference revealed that the expression level of <em>myb-like</em> could significantly affect <em>IAG</em> (encoding insulin-like androgenic gland hormone) expression. Furthermore, using the dual-luciferase reporter system, we confirmed that the MYB-LIKE protein could regulate the expression of <em>IAG</em> by binding to its promoter region directly. Our findings suggest that the transcription factor <em>myb-like</em> gene influences sex differentiation by regulating <em>IAG</em> expression in <em>P. trituberculatus</em>. Our results could provide clues to further study the regulatory mechanism of sex determination and differentiation, and lay the theoretical foundation for exploring single-sex breeding technology.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"599 ","pages":"Article 742173"},"PeriodicalIF":3.9,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143292595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}