Aquaculture最新文献

{"title":"BNIP3, targeted by miR-148 and miR-152, upregulates autophagy to inhibit Aeromonas hydrophila infection in the giant freshwater prawn Macrobrachium rosenbergii","authors":"Feifei Wang ,&nbsp;Binbin Lv ,&nbsp;Yang Liu ,&nbsp;Jing Wen ,&nbsp;Yanqiong Huang ,&nbsp;Yuting Deng ,&nbsp;Aiping Tan ,&nbsp;Ling Wang ,&nbsp;Yongzhong Liao ,&nbsp;Fei Zhao","doi":"10.1016/j.aquaculture.2025.743181","DOIUrl":"10.1016/j.aquaculture.2025.743181","url":null,"abstract":"<div><div>BNIP3 (Bcl-2/adenovirus E1B 19 kDa interacting protein 3) is a pro-apoptotic member of the Bcl-2 superfamily, that is implicated in autophagy during immune responses. To date, the mechanisms underlying BNIP3-mediated regulation of autophagy in crustaceans under pathogenic challenge remain poorly characterized. In this study, we demonstrated that BNIP3 in the giant freshwater prawn <em>Macrobrachium rosenbergii</em> (<em>Mr</em>BNIP3), which is critical for immune defense, is essential for inducing autophagy during in vivo bacterial infection. The open reading frame of <em>Mr</em>BNIP3 consists of 600 nucleotides that encode a 199-amino acid peptide containing multiple phosphorylation sites, a transmembrane domain, and a conserved BH3 motif. Phylogenetic analysis revealed high sequence similarity between <em>Mr</em>BNIP3 and invertebrate BNIP3 orthologs as well as clustering within the crustacean clade. <em>Mr</em>BNIP3 transcripts were ubiquitously expressed across all examined tissues, with maximal expression in muscle. Notably, <em>Mr</em>BNIP3 expression was significantly upregulated in the gill and hepatopancreas following stimulation with the pathogenic bacterium <em>Aeromonas hydrophila</em> or lipopolysaccharide. RNA interference-mediated knockdown of <em>Mr</em>BNIP3 suppressed autophagy activation and enhanced <em>A. hydrophila</em> invasion in <em>M. rosenbergii</em>. Furthermore, infection by <em>A. hydrophila</em> significantly downregulated microRNAs-148 and -152. Dual-luciferase reporter assays confirmed that these microRNAs co-targeted the 3′-untranslated region of <em>Mr</em>BNIP3 to repress its expression. Collectively, these findings identify <em>Mr</em>BNIP3 as a positive regulator of innate immunity in <em>M. rosenbergii</em> that is modulated through microRNA-dependent mechanisms.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743181"},"PeriodicalIF":3.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consequences of meiosis in first-generation hybrids derived from Megalobrama amblycephala (♀) and Culter alburnus (♂)","authors":"Yingying Yang ,&nbsp;Lang Qin ,&nbsp;Hongxuan Liang ,&nbsp;Jiawang Huang ,&nbsp;Ming Wen ,&nbsp;Yuxiang Wang ,&nbsp;Le Shen ,&nbsp;Jinhui Huang ,&nbsp;Xu Huang ,&nbsp;Fangzhou Hu ,&nbsp;Shi Wang ,&nbsp;Chang Wu ,&nbsp;Shaojun Liu","doi":"10.1016/j.aquaculture.2025.743187","DOIUrl":"10.1016/j.aquaculture.2025.743187","url":null,"abstract":"<div><div>Gynogenesis and distant hybridization are two techniques prevalently used for genetic breeding of fish. In artificially induced gynogenetic progeny, nuclear genetic material is derived from the duplication of the egg's chromosomes, making these progeny ideal models for exploring the meiotic characteristics of hybrid offspring. The hybrid BTF₁ was produced by crossing <em>Megalobrama amblycephala</em> (blunt snout bream, BSB, 2n = 48, ♀) with <em>Culter alburnus</em> (topmouth culter, TC, 2n = 48, ♂). The gynogenetic BTF₁ (GBTF₁) was induced using UV-irradiated sperm from common carp (<em>Cyprinus carpio</em>, 2n = 100, ♂), followed by chromosome doubling by cold shock. This process yielded two types of offspring with notable differences in appearance, designated GBTF₁-B and GBTF₁-M. The DNA content and chromosome number were analyzed, revealing that both GBTF₁-B and GBTF₁-M were diploids, each possessing 48 chromosomes. Meanwhile, the microsatellite amplification results showed that 39.92 % and 39.36 % of bands in GBTF₁-B and GBTF₁-M were consistent with both BSB and TC, 36.75 % and 23.77 % bands consistent with only BSB, 14.19 % and 20.97 % bands consistent with only TC, and 9.14 % and 15.90 % bands from mutation, respectively. The results indicated that BTF₁ produced heterozygous gametes with unevenly distributed genetic material of BSB and TC during meiosis. In addition, expression analysis of homologous genes showed that 15.3–20.33 % and 6.96–8.43 % differentially expressed genes (DEGs) in GBTF₁-B were biased to BSB and TC. In contrast, 10.40–14.39 % and 10.60–14.19 % DEGs in GBTF₁-M were biased to BSB and TC, respectively. The results suggested that the expression model of DEGs in GBTF₁-B was more biased towards BSB, whereas GBTF₁-M exhibited an intermediate expression pattern between BSB and TC. These findings demonstrated the heterogeneity of gametes produced by the first-generation hybrid fish. This research provides a genomic reference for understanding the outcomes of meiosis in hybrid fish and offers valuable insights into fish reproduction and breeding.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743187"},"PeriodicalIF":3.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145106023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenic function of the Lafa gene in Vibrio harveyi and its regulatory role in host immune response during infection of large yellow croaker (Larimichthys crocea)","authors":"Bin Li ,&nbsp;Zhihang Ouyang ,&nbsp;Zhenyuan Zhao ,&nbsp;Liang Miao ,&nbsp;Yang Du ,&nbsp;Jiong Chen","doi":"10.1016/j.aquaculture.2025.743186","DOIUrl":"10.1016/j.aquaculture.2025.743186","url":null,"abstract":"<div><div><em>Vibrio harveyi</em>, a ubiquitous pathogen in aquaculture, poses significant threats to numerous farmed aquatic species. Previous studies have demonstrated that bacterial flagella play a crucial role in adhesion and infection mechanisms, with the lateral flagellin lafA identified as a key virulence factor. To investigate the functional role of lafA in <em>V. harveyi</em>, this study constructed a gene deletion mutant (<em>ΔlafA</em>) from the wild-type strain LcV6 (Wt LcV6) and characterized its biological properties. Concurrently, transcriptomic analyses were conducted to identify expression differences between Wt LcV6 and <em>ΔlafA</em>. Additionally, the protective efficacy of <em>ΔlafA</em> was evaluated through intraperitoneal vaccination of large yellow croaker (<em>Larimichthys crocea</em>). Key findings revealed that <em>ΔlafA</em> exhibited no significant changes in growth kinetics compared to Wt LcV6 but showed enhanced biofilm formation capacity. Notably, the mutant strain displayed a marked reduction in motility. Transcriptomic analysis revealed a multifaceted bacterial adaptation strategy: significant upregulation of flagellar assembly pathway genes (<em>flhB</em>, <em>FliK</em>, <em>lafS</em>, <em>fliF</em>, <em>flhA</em>, and <em>flaE</em>) alongside <em>lafA</em> downregulation compensated for lateral flagella loss and maintained environmental adaptability, while sulfur-related genes exhibited marked divergence, with dsrC and AsrA being significantly upregulated, whereas <em>cysI</em>, <em>cysD</em>, <em>cysC</em>, <em>cysH</em>, and <em>cysN</em> were downregulated (<em>P &lt;</em> 0.05). Furthermore, vaccination with <em>ΔlafA</em> conferred a relative protection rate of 63.3 % against Wt LcV6 infection in large yellow croaker. The mutant also significantly increased specific IgM antibody titers and enhanced the enzymatic activities of catalase (CAT), alkaline phosphatase (AKP), and superoxide dismutase (SOD) in the livers of immunized fish. Moreover, it upregulated the expression of multiple immune-related genes, including <em>IgM</em>, <em>IgD</em>, <em>TGF-β1</em>, <em>IL-1β</em>, <em>TNF-α</em>, <em>MHC IIα</em>, <em>CD4</em>, and <em>CD8</em> in the livers, spleen, and kidney tissues of immunized fish. Collectively, these results underscore lafA as an essential virulence factor in <em>V. harveyi</em>. The <em>ΔlafA</em> mutant not only enhances immune responses in vaccinated fish but also represents a promising candidate for vaccine development, providing novel insights into the design of attenuated vaccines targeting <em>V. harveyi</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743186"},"PeriodicalIF":3.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of oxygen and antioxidants on the common carp (Cyprinus carpio L.) milt during short-term storage","authors":"Agnieszka Brzyszcz ,&nbsp;Beata Sarosiek ,&nbsp;Katarzyna Dryl ,&nbsp;Katarzyna Targońska ,&nbsp;Michał Blitek ,&nbsp;Dariusz Kucharczyk ,&nbsp;Wojciech Skorupa ,&nbsp;Masaya Morita ,&nbsp;Radosław K. Kowalski","doi":"10.1016/j.aquaculture.2025.743172","DOIUrl":"10.1016/j.aquaculture.2025.743172","url":null,"abstract":"<div><div>This study explores the impact of oxygen and antioxidants in an immobilizing buffer on short-term storage of common carp sperm. Sperm from five carp individuals underwent short-term storage for 10 days without dilution (Control), mixed with buffer TLP alone (TLP), or with antioxidants: 5 mM glutathione (Glut), 5 mM ascorbic acid (VitC), 1.5 mg/mL vitamin E (VitE), and a mix of all mentioned antioxidants (Mix). Diluted milt samples were stored at 4 °C in either open Eppendorf tubes within oxygen-filled bags (refreshed every 24 h) or closed tubes under limited aerobic conditions. All samples were gently mixed twice daily to prevent spermatozoa sedimentation. Computer-assisted sperm analysis (CASA) was employed to measure the curvilinear velocity (VCL) and total motility (MOT) of stored samples. Live/Dead double staining kit assessed sperm membrane integrity. After 10 days of storage, a fertility trial was conducted to compare preserved milt with freshly collected sperm samples (<em>n</em> = 3). In samples stored in limited aerobic conditions, the VCL parameter in TLP, Glut, and VitE buffers remained steady. Oxygen altered MOT and VCL, with the best variants of diluents (TLP and VitE) showing a decrease from 90 % to about 80 %. In samples with the addition of ascorbic acid and the antioxidant mixture, a loss of viability was observed after 6 days. On the other hand, membrane integrity in limited aerobic conditions (in buffers TLP, Glut, and VitE) remained unaffected to day 10. Fertilization success, measured by hatching rate, indicated that diluted common carp milt, stored for 10 days in aerobic conditions, maintained its fertilizing potential. This study demonstrates the impact of oxygen utilization and the potential beneficial effect of antioxidant supplementation on the short-term storage of common carp milt in aquaculture practices. Correlation analysis conducted in this study revealed strong positive relationships between hatching rate and motility parameters, such as ALH and total motility, highlighting the importance of these factors in successful common carp fertilization.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743172"},"PeriodicalIF":3.9,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accurate and real-time fish feeding intensity scoring using channel convolution GLU and SMFA attention fusion in recirculating aquaculture system","authors":"Chenjian Liu ,&nbsp;Xinting Yang ,&nbsp;Baoliang Liu ,&nbsp;Zhenxi Zhao ,&nbsp;Pingchuan Ma ,&nbsp;Tingting Fu ,&nbsp;Weichen Hu ,&nbsp;Xiaoqiang Gao ,&nbsp;Chao Zhou","doi":"10.1016/j.aquaculture.2025.743180","DOIUrl":"10.1016/j.aquaculture.2025.743180","url":null,"abstract":"<div><div>In aquaculture, real-time quantification of fish feeding intensity is critical for developing scientific feeding strategies. Previous deep learning-based studies primarily focused on rough recognition of feeding and non-feeding patterns. However, with the growing demand for intelligent feeding systems, there is an urgent need for precise and quantitative assessment of fish feeding intensity. To address this, this study proposes CS-TransNeXt, a fish feeding intensity scoring model that integrates Channel Convolution GLU (CCGLU) and Self-Modulation Feature Aggregation (SMFA), which can precisely quantify feeding intensity into 10 scoring scales (1−10). Specifically, the CCGLU module is introduced into the TransNeXt, so as to enhance local feature modeling by fusing multi-scale Depthwise Convolutions with channel attention. Meanwhile, the SMFA replaces the original multi-head self-attention in TransNeXt, enabling adaptive weight adjustment through variance-based dynamic parameters of global-local features. Experimental results demonstrate that the proposed CS-TransNeXt achieves a Top-1 Accuracy of 95.25 %, an F1-Score of 95.30 %, outperforming the baseline TransNeXt-micro by 4.00 % in accuracy. Meanwhile, it is only 17.60 M, and provides a novel method for high-precision quantitative scoring of fish feeding intensity, offering significant practical value for the development of intelligent feeding systems.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743180"},"PeriodicalIF":3.9,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic inbreeding evaluation and its effects on phenotype and selection accuracy of resistance to Edwardsiella tarda in Turbot (Scophthalmus maximus)","authors":"Ding Lyu ,&nbsp;Qiang Fu ,&nbsp;Mianyu Liu ,&nbsp;Yulong Hu ,&nbsp;Guanzheng Lyu ,&nbsp;Weiji Wang","doi":"10.1016/j.aquaculture.2025.743178","DOIUrl":"10.1016/j.aquaculture.2025.743178","url":null,"abstract":"&lt;div&gt;&lt;div&gt;To accurately evaluate the effects of inbreeding on phenotypes and selection accuracy, this study conducted resequencing on a population of turbot (&lt;em&gt;Scophthalmus maximus&lt;/em&gt;) involved in resistance testing against &lt;em&gt;Edwardsiella tarda&lt;/em&gt; and calculated individual inbreeding coefficients (&lt;em&gt;F&lt;/em&gt;) based on SNP homozygosity (&lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mi&gt;HOM&lt;/mi&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt;), diagonal of the SNP-based genomic relationship matrix (&lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mi&gt;GRM&lt;/mi&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt;), and runs of homozygosity (ROH) with varying length thresholds (10 kb, 100 kb, 500 kb, 1000 kb; denoted as &lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;ROH&lt;/mi&gt;&lt;mn&gt;10&lt;/mn&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt;, &lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;ROH&lt;/mi&gt;&lt;mn&gt;100&lt;/mn&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt;, &lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;ROH&lt;/mi&gt;&lt;mn&gt;500&lt;/mn&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt;, &lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;ROH&lt;/mi&gt;&lt;mn&gt;1000&lt;/mn&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt;). The impact of &lt;em&gt;F&lt;/em&gt; derived from these methods on phenotypic traits was assessed, and the potential improvement in genomic selection accuracy by incorporating inbreeding depression (ID) effects into the genetic evaluation model was examined. The average values of &lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mi&gt;HOM&lt;/mi&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt; and &lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mi&gt;GRM&lt;/mi&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt; were near zero (−0.006 ± 0.107 and 0.004 ± 0.064, respectively), while &lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;ROH&lt;/mi&gt;&lt;mn&gt;10&lt;/mn&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt; exhibited the highest mean value (0.103 ± 0.064). As the ROH fragment length increased, the ROH-based &lt;em&gt;F&lt;/em&gt; gradually decreased, with &lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;ROH&lt;/mi&gt;&lt;mn&gt;1000&lt;/mn&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt; averaging 0.005 ± 0.011. Regression analysis revealed negative regression coefficients across all methods, but only &lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;ROH&lt;/mi&gt;&lt;mn&gt;100&lt;/mn&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt; and &lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;ROH&lt;/mi&gt;&lt;mn&gt;500&lt;/mn&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt; showed significant effects on phenotypic traits (&lt;em&gt;p&lt;/em&gt; &lt; 0.05 and &lt;em&gt;p&lt;/em&gt; &lt; 0.01, respectively). &lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;ROH&lt;/mi&gt;&lt;mn&gt;1000&lt;/mn&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt; exhibited a marginal association with the phenotype (&lt;em&gt;p&lt;/em&gt; &lt; 0.10). The estimated ID effects on resistance to &lt;em&gt;E. tarda&lt;/em&gt; were largest for &lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;ROH&lt;/mi&gt;&lt;mn&gt;1000&lt;/mn&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt; (10.871 %), followed by &lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;ROH&lt;/mi&gt;&lt;mn&gt;500&lt;/mn&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt; (6.033 %) and &lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;ROH&lt;/mi&gt;&lt;mn&gt;100&lt;/mn&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt; (2.443 %). The prediction accuracy of GBLUP was 0.539. The accuracies of GBLUP-&lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;ROH&lt;/mi&gt;&lt;mn&gt;100&lt;/mn&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt; and GBLUP-&lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;F&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;ROH&lt;/mi&gt;&lt;mn&gt;1000&lt;/mn&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt; were nearly identical","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743178"},"PeriodicalIF":3.9,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consequences of climate change on marine fish reproduction in aquaculture farms: Unveiling a novel interaction between TRPV1 and follicle-stimulating hormone","authors":"Kyle Dominic Barnuevo ,&nbsp;Mariel Galotta ,&nbsp;Sipra Mohapatra ,&nbsp;Oga Sato ,&nbsp;Hironori Katoh ,&nbsp;Takehiko Itoh ,&nbsp;Naoki Nagano ,&nbsp;Michiya Matsuyama ,&nbsp;Tapas Chakraborty ,&nbsp;Kohei Ohta","doi":"10.1016/j.aquaculture.2025.743177","DOIUrl":"10.1016/j.aquaculture.2025.743177","url":null,"abstract":"<div><div>Temperature exerts a profound influence on the reproductive physiology of ectotherms such as fish, rendering it imperative to comprehend the long-term consequences and underlying mechanisms of temperature-related reproductive modifications in the context of a warming climate. Utilizing chub mackerel (<em>Scomber japonicus</em>) as a model, this study examined how variations in environmental temperature impact reproductive development, gonadotropin production, and the function of thermosensory pathways. Comparative breeding data collected from 2021 to 2024 across two aquaculture facilities, exhibiting temperature differences of up to 3.5 °C, revealed that broader temperature fluctuations may accelerate initial reproductive development while potentially impairing later reproductive stages, thereby diminishing fertilization rates and hatchability. The gene <em>sjtrpv1</em>, a chub mackerel homolog of mammalian TRPV 1, was cloned and characterized. It revealed its high conservation and widespread tissue expression, with sex-dependent and seasonal variations in expression patterns. <em>In situ</em> hybridization experiments demonstrated a strong colocalization of <em>sjtrpv1</em> with <em>fshb</em> in FSH-producing cells within the <em>proximal pars distalis</em>, implying its involvement in transmitting thermal signals to the reproductive axis. Seasonal analyses showed a negative correlation between <em>sjtrpv1</em> expression and environmental temperature and a positive correlation with <em>fshb</em> levels, suggesting a role in synchronizing reproductive timing with seasonal thermal cues. These findings imply that <em>sjtrpv1</em> likely mediates the influence of temperature on reproductive processes via regulation of <em>fshb,</em> thereby affecting reproductive timing and success in chub mackerel. Understanding these mechanisms will facilitate the optimization of thermal management strategies in sustainable aquaculture and provide valuable insights into the reproductive adaptation of wild fish populations amid increasing ocean temperatures driven by climate change.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743177"},"PeriodicalIF":3.9,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking aquaculture performance: Beyond cost efficiency and the role of heterogeneity","authors":"Marta Arbelo-Pérez ,&nbsp;Violeta De Vera ,&nbsp;Antonio Arbelo ,&nbsp;Pilar Pérez-Gómez","doi":"10.1016/j.aquaculture.2025.743166","DOIUrl":"10.1016/j.aquaculture.2025.743166","url":null,"abstract":"<div><div>In recent years, the aquaculture industry has faced increasing competition, which negatively impacts firm performance. Despite the growing importance of the industry, there is limited research evaluating both cost and profit efficiency while considering heterogeneity across firms. This study aims to fill this gap by estimating the efficiency of costs and profits in aquaculture in Spain via a stochastic frontier Bayesian model with random coefficients. This methodological approach enables a more accurate and differentiated assessment of firm performance by explicitly incorporating technological heterogeneity across firms. The results show that cost efficiency significantly exceeds profit efficiency, which indicates that aquaculture firms manage their costs more efficiently than their revenues. From the resource-based view (RBV), this suggests that firms may not be fully leveraging their unique resources and capabilities to increase revenue, possibly due to strategic or market limitations. The findings indicate that firms could enhance performance by more than 50 % if they achieved full profit efficiency, which highlights the critical role of resource optimization. These insights have significant implications for both scholars and managers, and highlight the need to identify, develop, and leverage internal resources to strengthen the competitive position of aquaculture firms.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743166"},"PeriodicalIF":3.9,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive performance evaluation of Vibrio resistance strains from selective breeding in the Pacific oyster, Crassostrea gigas","authors":"Ben Yang ,&nbsp;Deqi Sun ,&nbsp;Chengxun Xu ,&nbsp;Qi Li ,&nbsp;Shikai Liu","doi":"10.1016/j.aquaculture.2025.743179","DOIUrl":"10.1016/j.aquaculture.2025.743179","url":null,"abstract":"<div><div>Understanding the production performance of newly developed strains in the field is essential for the successful implementation of breeding project and promotes the sustainable development of aquaculture. In this study, a comparative analysis of growth and survival traits between the selectively bred <em>Vibrio</em>-resistant strain and the control population across multiple growth stages and geographic locations was conducted to evaluate the impact of selective breeding and environmental variation on these economically critical traits in <em>Crassostrea gigas</em>. The results showed no significant differences in performance during the larval stages between the <em>Vibrio</em>-resistant strain and the control population. In field, the <em>Vibrio</em>-resistant oysters showed significantly higher shell heights compared to the control population, notably in the Rushan sea area. The <em>Vibrio</em>-resistant oysters consistently demonstrated superior survival rates, particularly in the Rongcheng area. Genotype and environmental factors significantly influenced growth and survival, with a higher impact of environmental factors. The interaction between environment and genotype was relatively weak, but there was a significant impact on survival at 300 days and growth at 400 days (<em>P</em> &lt; 0.05). The aggregate breeding value obtained by economically weighting the breeding values of growth and survival traits revealed that the <em>Vibrio</em>-resistant strain possessed higher economic value and application potential. This study provides valuable empirical data on resistance breeding in oysters, which enhances our understanding of the progress of oyster resistance breeding, and facilitates the cultivation of more robust and resistant oyster varieties.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743179"},"PeriodicalIF":3.9,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salinity acclimatization enhances Atlantic salmon resistance to Tenacibaculum finnmarkense: Skin infection and host response","authors":"Marte Fredriksen ,&nbsp;Kristense Solheim ,&nbsp;Lene Sveen ,&nbsp;Gerrit Timmerhaus ,&nbsp;Sergey Afanasyev ,&nbsp;Aleksei Krasnov ,&nbsp;Miroslava Hansen ,&nbsp;Are Nylund ,&nbsp;Sverre Bang Småge ,&nbsp;Christian Karlsen","doi":"10.1016/j.aquaculture.2025.743174","DOIUrl":"10.1016/j.aquaculture.2025.743174","url":null,"abstract":"<div><div>Tenacibaculosis is a severe bacterial ulcerative disease affecting Atlantic salmon aquaculture. Fish are most susceptible to infection during the first weeks at sea, with higher risks associated with low water temperatures. This study investigated whether water salinity prior to seawater transfer could mitigate disease progression. Two production strategies were compared: gradual acclimatization in low seawater salinity (LSS, 26 ppt) and continuous freshwater (F, 0 ppt) production until seawater transfer. Three challenge trials were conducted with <em>Tenacibaculum finnmarkense</em>, the first with freshwater smolt transferred directly to seawater, then two trials with postsmolts that had been held for a further 25 or 50 days in either F or LSS conditions prior to seawater transfer. Mortality rates were recorded as follows: 15 % in the first trial with smolts, 53 % (F) and 22 % (LSS) in the second trial, and 23 % (F) and 13 % (LSS) in the third trial. Histopathological analysis of skin ulcers from infected fish revealed a clear preference of <em>T. finnmarkense</em> for collagen-rich tissues. The bacterium may exploit the collagen matrix to facilitate translocation into underlying tissues, where it is associated with necrotic muscle fibers and thrombosis. Transcriptome analysis showed largely similar gene expression changes in the dermis and epidermis of infected fish, characterized by an acute inflammatory response. Comparison between the F and LSS groups provided insights into the mechanisms underlying increased resistance. In uninfected LSS fish, immune gene expression was generally lower, while epidermal cell proliferation was more active, alongside the upregulation of diverse collagens, collagen chaperones, and modifying enzymes. Following infection, gene expression differences between groups diminished in the epidermis and nearly disappeared in the dermis. However, the initial disparities contributed to a proportionally greater response in the LSS group. Acclimatization in LSS water may enhance resistance to <em>T. finnmarkense</em> infection potentially improving survival in aquaculture settings.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743174"},"PeriodicalIF":3.9,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}